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Effects of soyhulls with different particle size on the growth performance, blood indices and gut microbiota of yellow feather broilers. 不同粒径的豆壳对黄羽肉鸡生长性能、血液指数和肠道微生物群的影响
IF 2 3区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-04-01 Epub Date: 2024-02-28 DOI: 10.1080/00071668.2024.2308276
X Chen, C Luo, Y Liu, T Li, H Zhang, X Feng

1. The objective of this study was to determine the effects of soyhulls with different particle sizes on the growth performance, blood indices and gut microbiota of yellow feather broilers.2. Total of 240 healthy, one-day-old, yellow feather broilers were randomly divided into four groups, with six pen replicates within each group and ten birds per pen. The control group birds were fed the basal diet (Control). For the treatment groups, 5% soyhulls with different particle sizes were included in the basal diet. The particle size geometric mean diameters (dgw) of the soyhulls in the three treatment groups were 299.69 μm (LowPS), 489.85 μm (MediumPS) and 734.83 μm (HighPS) with geometric standard deviation (Sgw) 1.75 μm, 1.62 μm and 1.67 μm, respectively.3. Results showed that the growth performance variables and organ indices were not different among the four groups. The MediumPS group had increased TG, T-CHO, ALT, HDL-C, and GSH-PX levels and decreased T-AOC levels, whereas LowPS and HighPS groups had increased HDL-C and GSH-PX levels (p < 0.05). Microbial diversity analysis showed that the intestinal microbiota of yellow feather broilers mainly included Firmicutes and Bacteroidetes. Inclusion of 5% soyhulls with different particle size had no effect on alpha diversity indices of caecal microbiota. The HighPS group had significantly higher relative abundance of Firmicutes spp. and lower Bacteroidetes spp. compared with the LowPS and MediumPS group but this was not different from the Control group. The relative abundance of Cyanobacteria spp. was significantly higher in the HighPS group than the other three groups. LEfSe analysis showed that there were more enriched biomarker taxa in the groups with soyhulls than the control group.4. Overall, the inclusion of soyhulls with different particle sizes had limited effects on growth performance, blood indices and caecal microbiota composition of yellow feather broilers.

1.本研究的目的是确定不同粒径的豆壳对黄羽肉鸡生长性能、血液指数和肠道微生物群的影响。 2. 将 240 只健康、一天龄的黄羽肉鸡随机分为 4 组,每组 6 个重复,每栏 10 只。对照组饲喂基础日粮(对照组)。对于处理组,在基础日粮中添加不同粒径的 5%豆壳。三个处理组的豆壳粒径几何平均直径(dgw)分别为 299.69 μm(LowPS)、489.85 μm(MediumPS)和 734.83 μm(HighPS),几何标准偏差(Sgw)分别为 1.75 μm、1.62 μm 和 1.67 μm。 结果表明,四个处理组的生长性能变量和器官指数没有差异。中PS组的TG、T-CHO、ALT、HDL-C和GSH-PX水平升高,T-AOC水平降低,而低PS组和高PS组的HDL-C和GSH-PX水平升高(p 固着菌和类杆菌)。加入不同粒径的 5%大豆壳对盲肠微生物群的α多样性指数没有影响。与低粒径组和中粒径组相比,高粒径组的固着菌属相对丰度明显较高,而拟杆菌属相对丰度较低,但与对照组相比没有差异。蓝藻属的相对丰度在高浓度多糖组明显高于其他三组。4. 总体而言,添加不同粒径的豆壳对黄羽肉鸡的生长性能、血液指数和盲肠微生物群组成的影响有限。
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引用次数: 0
Genetic mapping of the novel congenital loco locus on chromosome Z in Silkie Fowl chickens. 乌骨鸡 Z 染色体上新型先天基因座的遗传图谱。
IF 2 3区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-04-01 Epub Date: 2024-01-17 DOI: 10.1080/00071668.2023.2297806
K Shiina, E Kobayashi

1. 'Congenital loco' is a disorder in birds expressed at hatching, and the primary symptom is dorsal backward bending of the neck. It is a recessive disease caused by a mutation in a specific genetic locus. The following study identified a novel locus associated with congenital loco in Silkie Fowl chickens.2. Normal and congenital loco-affected Silkie Fowl chicks exhibited no differences in the frequencies of markers on chromosome 12 adjacent to the congenital loco locus reported in a previous study in Rhode Island Red chickens. Sex determination of congenital loco-affected chicks revealed that they were female only.3. Bulked segregant analyses using next-generation sequencing narrowed the causative region of congenital loco to approximately 3.3 Mb between bases 9,569,012 and 12,863,792 on chromosome Z.

1.先天性驼背 "是鸟类在孵化时表现出的一种疾病,主要症状是颈部背向后弯曲。这是一种隐性疾病,由特定基因位点的突变引起。以下研究确定了一个与乌骨鸡先天性位点相关的新位点2:正常乌骨鸡雏鸡和受先天性位点影响的乌骨鸡雏鸡在与先前罗德岛红鸡研究中报告的先天性位点相邻的 12 号染色体上的标记频率上没有差异。先天性位点受影响雏鸡的性别鉴定结果显示,这些雏鸡仅为雌性。 利用下一代测序技术进行的大量分离分析将先天性位点的致病区域缩小到 Z 染色体上 9,569,012 至 12,863,792 碱基之间约 3.3 Mb 的范围内。
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引用次数: 0
Novel sfericase protease improves amino acid digestibility of soybean meal and rapeseed meal in broiler chickens. 新型干酪蛋白酶提高肉鸡对豆粕和菜籽粕的氨基酸消化率
IF 2 3区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-04-01 Epub Date: 2024-01-24 DOI: 10.1080/00071668.2023.2295348
H Liu, S Wang, J-O Sorbara, M Umar-Faruk, A J Cowieson

1. Two experiments were conducted to explore the effects of an exogenous sfericase protease on the apparent ileal nutrient digestibility of soybean meal (SBM) and rapeseed meal (RSM) in broiler chickens.2. In each experiment, a total of 256 sixteen-day-old male Cobb 500 broilers were fed one of four semi-purified experimental diets, comprising two different batches (A and B) of samples for either SBM (Exp. 1) or RSM (Exp. 2) without or with an exogenous sfericase (0 or 30,000 NFP/kg). Each experimental diet was fed to eight replicate pens of broiler chickens from 16 to 21 d of age (eight birds per cage), and ileal digesta were collected for measuring the digestibility coefficients.3. In Exp. 1, the amino acid digestibility was greater (P < 0.05) in SBM B compared with SBM A for Arg and Val, and a similar trend (P < 0.1) was observed for Tyr, Leu and Thr. Exogenous sfericase increased (P < 0.10) digestibility of most of amino acids except Gly, His and Trp. There was an interaction between SBM source and sfericase, whereby digestibility of P, N and Asp was increased by sfericase for SBM B but not for SBM A. In Exp. 2, there was no interaction (P > 0.05) between RSM source and sfericase for ileal nutrient digestibility. Digestibility was greater in RSM A compared to RSM B for all non-essential AA and most essential AA (except for Trp), while the reverse was noted for Ca and P (P < 0.05). Exogenous sfericase increased (P < 0.1) digestibility for all amino acids except Cys and Met.4. In conclusion, the current studies showed that both SBM and RSM batches influenced amino acid digestibility. Sfericase protease supplementation increased amino acid digestibility for both SBM and RSM. The digestibility effects were greater in the SBM batch with low digestibility for N and Asp which was in line with an increase in P digestibility.

1.我们进行了两项实验,以探讨外源干扰蛋白酶对肉鸡大豆粉(SBM)和菜籽粕(RSM)表观回肠营养消化率的影响。在每个实验中,总共饲喂了 256 只 16 日龄的雄性 Cobb 500 肉鸡,从四种半纯化实验日粮中选择一种,这四种日粮包括两种不同批次(A 和 B)的 SBM(Exp.1)或 RSM(Exp.2)样品,不含或含外源铁蛋白酶(0 或 30,000 NFP/kg)。每种试验日粮饲喂 8 个重复栏的 16 至 21 日龄肉鸡(每笼 8 只鸡),收集回肠消化物以测量消化率系数。 在试验 1 中,RSM 源和阿魏酸酶对回肠营养物质消化率的影响更大(P P P > 0.05)。与 RSM B 相比,RSM A 对所有非必需 AA 和大多数必需 AA(Trp 除外)的消化率更高,而对 Ca 和 P 的消化率则相反(P P P
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引用次数: 0
Antiviral effect of palmatine against infectious bronchitis virus through regulation of NF-κB/IRF7/JAK-STAT signalling pathway and apoptosis. 巴马汀通过调节 NF-κB/IRF7/JAK-STAT 信号通路和细胞凋亡对传染性支气管炎病毒的抗病毒作用
IF 2 3区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-04-01 Epub Date: 2024-01-26 DOI: 10.1080/00071668.2023.2296929
W Zhang, Y Chen, F Yang, H Zhang, T Su, J Wang, Y Zhang, X Song

1. Infectious bronchitis virus (IBV), a gamma-coronavirus, can infect chickens of all ages and leads to an acute contact respiratory infection. This study evaluated the anti-viral activity of palmatine, a natural non-flavonoid alkaloid, against IBV in chicken embryo kidney (CEK) cells.2. The half toxic concentration (CC50) of palmatine was 672.92 μM, the half inhibitory concentration (IC50) of palmatine against IBV was 7.76 μM and the selection index (SI) was 86.74.3. Mode of action assay showed that palmatine was able to directly inactivate IBV and inhibited the adsorption, penetration and intracellular replication of IBV.4. Palmatine significantly upregulated TRAF6, TAB1 and IKK-β compared with the IBV-infected group, leading to the increased expressions of pro-inflammatory cytokines IL-1β and TNF-α in the downstream NF-κB signalling pathway.5. Palmatine significantly up-regulated the levels of MDA5, MAVS, IRF7, IFN-α and IFN-β in the IRF7 pathway, inducing type I interferon production. It up-regulated the expression of 2'5'-oligoadenylate synthase (OAS) in the JAK-STAT pathway.6. IBV infection induced cell apoptosis and palmatine-treatment delayed the process of apoptosis by regulation of the expression of apoptosis-related genes (BAX, BCL-2, CASPASE-3 and CASPASE-8).7. Palmatine could exert anti-IBV activity through regulation of NF-κB/IRF7/JAK-STAT signalling pathways and apoptosis, providing a theoretical basis for the utilisation of palmatine to treat IBV infection.

1.传染性支气管炎病毒(IBV)是一种γ-冠状病毒,可感染所有年龄的鸡,并导致急性接触性呼吸道感染。2. 巴马汀的半毒浓度(CC50)为 672.92 μM,对 IBV 的半抑制浓度(IC50)为 7.76 μM,选择指数(SI)为 86.74.3。作用模式分析显示,巴马汀能够直接灭活 IBV,并抑制 IBV 的吸附、穿透和细胞内复制。与 IBV 感染组相比,巴马汀能明显上调 TRAF6、TAB1 和 IKK-β,导致下游 NF-κB 信号通路中促炎细胞因子 IL-1β 和 TNF-α 的表达增加。 巴马汀能明显上调 IRF7 通路中 MDA5、MAVS、IRF7、IFN-α 和 IFN-β的水平,诱导 I 型干扰素的产生。它上调了 JAK-STAT 通路中 2'5'-oligoadenylate synthase(OAS)的表达。巴马汀可通过调节 NF-κB/IRF7/JAK-STAT 信号通路和细胞凋亡发挥抗 IBV 活性,为利用巴马汀治疗 IBV 感染提供了理论依据。
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引用次数: 0
Effects of different stocking densities on the development of reproductive and immune functions in young breeder pigeons during the rearing period. 不同饲养密度对育雏期种鸽生殖和免疫功能发育的影响
IF 2 3区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-04-01 Epub Date: 2024-02-09 DOI: 10.1080/00071668.2024.2308273
P Xie, L Wang, J Zhu, Y Liu, M Wei, D Gong, T Liu

1. Stocking density (SD) is closely related to animal performance. This experiment was designed to evaluate the development of reproductive and immune functions of young pigeons under different SDs.2. A total of 288 (half male and half female) 40-day-old pigeons (body weight 400 ± 15 g) were allocated into four groups: High stocking density (HSD; 0.308 m3/bird), standard stocking density (SD; 0.616 m3/bird), and low stocking density (LSD; 1.232 m3/bird) and a caged (control; 0.04125 m3/bird). Every group had six replicates of the same sex.3. The results showed that caged male pigeons had the highest testis index, testosterone content, and gene expression of the androgen receptor gene. LSD treatment induced the highest concentrations of oestradiol, progesterone and mRNA levels of reproductive hormone receptor genes in female pigeons. In male pigeons, the spleen index (organ weight calculated as a percentage of total body weight) showed a peak level (0.09 ± 0.020) in the LSD group, and the thymus index peaked (0.23 ± 0.039) in SD group. However, the index for ovary, spleen, thymus and bursa of Fabricius in female pigeons showed no significant changes among different groups.4. The IL-1β, IL-8, IFN-γ, TGF-β and toll-like receptor 2 (TLR-2) mRNA levels reached their maximum values in both male and female pigeon spleens in the LSD group.5. Young male pigeons housed in cages showed increased testicular development while low stocking density increased the development of reproductive function in young female pigeons. A larger activity space could help enhance the immune function of both male and female pigeons.

1.饲养密度(SD)与动物性能密切相关。本实验旨在评估幼鸽在不同放养密度下生殖和免疫功能的发育情况。 2. 将 288 只(雌雄各半)40 日龄的幼鸽(体重 400 ± 15 g)分为四组:高放养密度组(HSD;0.308 m3/羽)、标准放养密度组(SD;0.616 m3/羽)、低放养密度组(LSD;1.232 m3/羽)和笼养组(对照组;0.04125 m3/羽)。结果表明,笼养雄鸽的睾丸指数、睾酮含量和雄激素受体基因的基因表达量最高。雌鸽的雌二醇、孕酮浓度和生殖激素受体基因的 mRNA 水平最高。雄鸽的脾脏指数(器官重量占总重量的百分比)在LSD组达到峰值(0.09 ± 0.020),胸腺指数在SD组达到峰值(0.23 ± 0.039)。4.LSD组雌、雄鸽脾脏中IL-1β、IL-8、IFN-γ、TGF-β和toll样受体2(TLR-2)mRNA水平均达到最高值。 5.笼养幼雄鸽睾丸发育加快,低密度饲养幼雌鸽生殖机能发育加快。较大的活动空间有助于提高雄鸽和雌鸽的免疫功能。
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引用次数: 0
Examining the relationship between different naturally-occurring maxillary beak shapes and their ability to cause damage in commercial laying hens. 研究不同自然发生的上颌喙形状与其对商品蛋鸡造成伤害的能力之间的关系。
IF 2 3区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-04-01 Epub Date: 2024-02-09 DOI: 10.1080/00071668.2024.2308279
S Struthers, I C Dunn, J J Schoenebeck, V Sandilands

1. Using chicken models to avoid unnecessary harm, this study examined the relationship between naturally-occurring maxillary (top) beak shapes and their ability to cause pecking damage.2. A selection of 24 Lohmann Brown laying hens from a total population of 100 were sorted into two groups based on their maxillary beak shape, where 12 were classified as having sharp beaks (SB) and 12 as having blunt beaks (BB).3. All hens were recorded six times in a test pen which contained a chicken model (foam block covered with feathered chicken skin) and a video camera. During each test session, the number of feathers removed from the model, the change in skin and block weight (proxies for tissue damage) and the percentage of successful pecks (resulting in feather and/or tissue removal) were recorded.4. SB hens removed more feathers from the model and had a greater change in skin weight than BB hens. The mean number of pecks made at the model did not differ between the beak shape groups; however, SB hens had a greater percentage of successful pecks, resulting in feather and/or tissue removal, compared to BB hens.5. In conclusion, SB hens were more capable of removing feathers and causing damage. Birds performed more successful pecks resulting in feather and/or tissue removal as they gained experience pecking at the model.

1.为了避免不必要的伤害,本研究利用鸡的模型研究了自然形成的上颌(顶部)喙形状与其造成啄伤的能力之间的关系。 3.所有母鸡在一个装有鸡模型(泡沫塑料块上覆盖着带羽毛的鸡皮)和摄像机的试验围栏中被记录了六次。4. 与 BB 型母鸡相比,SB 型母鸡从模型上啄掉的羽毛更多,鸡皮重量的变化也更大。喙形组之间啄击模型的平均次数没有差异;但与 BB 型母鸡相比,SB 型母鸡成功啄击的百分比更高,导致羽毛和/或组织脱落。 总之,SB 型母鸡更有能力拔除羽毛并造成破坏。随着啄模型经验的积累,啄羽毛和/或啄组织的成功率越来越高。
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引用次数: 0
Disturbance of mitochondrial dynamics in myocardium of broilers with pulmonary hypertension syndrome. 肺动脉高压综合征肉鸡心肌线粒体动力学紊乱
IF 2 3区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-04-01 Epub Date: 2024-02-21 DOI: 10.1080/00071668.2024.2308277
W Zhang, M Li, X Ye, M Jiang, X Wu, Z Tang, L Hu, H Zhang, Y Li, J Pan

1. The following study investigated the relationship between pulmonary hypertension syndrome (PHS) and mitochondrial dynamics in broiler cardiomyocytes.2. An animal model for PHS was established by injecting broiler chickens with CM-32 cellulose particles. Broiler myocardial cells were cultured under hypoxic conditions to establish an in vitro model. The ascites heart index, histomorphology, mitochondrial ultrastructure, and mitochondrial dynamic-related gene and protein expression were evaluated.3. The myocardial fibres from PHS broilers had wider spaces and were wavy and twisted and the number of mitochondria increased. Compared with the control group, the gene and protein expression levels were decreased for Opa1, Mfn1, and Mfn2 in the myocardium of PHS broilers. The gene and protein expression was significantly increased for Drp1 and Mff.4. This study showed that PHS in broilers may cause myocardial mitochondrial dysfunction, specifically by diminishing mitochondrial fusion and enhancing fission, causing disturbances in the mitochondrial dynamics of the heart.

1.以下研究调查了肺动脉高压综合征(PHS)与肉鸡心肌细胞线粒体动力学之间的关系。 2. 通过向肉鸡注射 CM-32 纤维素颗粒,建立了肺动脉高压综合征的动物模型。在缺氧条件下培养肉鸡心肌细胞,建立体外模型。评估了腹水心指数、组织形态学、线粒体超微结构以及线粒体动态相关基因和蛋白的表达。 PHS 肉鸡的心肌纤维间隙变宽,呈波浪状扭曲,线粒体数量增加。与对照组相比,PHS 肉鸡心肌中 Opa1、Mfn1 和 Mfn2 的基因和蛋白表达水平下降。本研究表明,PHS 可导致肉鸡心肌线粒体功能障碍,具体表现为线粒体融合减少,裂变增加,从而引起心脏线粒体动力学紊乱。
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引用次数: 0
Effect of quercetin on granulosa cells development from hierarchical follicles in chicken. 槲皮素对鸡分级卵泡颗粒细胞发育的影响。
IF 2 3区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-02-01 Epub Date: 2023-10-23 DOI: 10.1080/00071668.2023.2264792
T Li, Y Feng, Z Chen, Q Hou, B R Serrano, A R Barcenas, P Wu, W Zhao, M Shen

1. The bioflavonoid quercetin is a biologically active component, but its functional regulation of granulosa cells (GCs) during chicken follicular development is little studied. To investigate the effect of quercetin on follicular development in laying hens, an in vitro study was conducted on granulosa cells from hierarchical follicles treated with quercetin.2. The effect of quercetin on cell activity, proliferation and apoptosis of granulosa cells was detected by CCK-8, EdU and apoptosis assays. The effect on progesterone secretion from granulosa cells was investigated by enzyme-linked immunosorbent assay (ELISA). Expression of proliferating cell nuclear antigen (PCNA) mRNA and oestrogen receptors (ERs), as well as the expression of steroid acute regulatory protein (StAR), cytochrome P450 cholesterol side chain cleavage enzyme (P450scc) and 3β-hydroxysteroid dehydrogenase (3β-HSD) mRNA during progesterone synthesis, were measured by real-time quantitative polymerase chain reaction (RT-qPCR). PCNA, StAR and CYP11A1 protein expression levels were detected using Western blotting (WB).3. The results showed that treatment with quercetin in granulosa cells significantly enhanced cell vitality and proliferation, reduced apoptosis and promoted the expression of gene and protein levels of PCNA. The levels of progesterone secretion increased significantly following quercetin treatment, as did the expression levels of StAR and CYP11A1 using the Western Blot (WB) method.4. The mRNA expression levels of ERα were significantly upregulated in the 100 ng/ml and 1000 ng/ml quercetin-treated groups, while there was no significant difference in expression levels of ERβ mRNA.

1.生物类黄酮槲皮素是一种生物活性成分,但对其在鸡卵泡发育过程中对颗粒细胞(GC)的功能调控研究较少。为了研究槲皮素对蛋鸡卵泡发育的影响,对槲皮素处理的分级卵泡颗粒细胞进行了体外研究。采用CCK-8、EdU和凋亡测定法检测槲皮素对颗粒细胞活性、增殖和凋亡的影响。采用酶联免疫吸附试验(ELISA)研究了对颗粒细胞孕酮分泌的影响。采用实时定量聚合酶链反应(RT-qPCR)检测孕酮合成过程中增殖细胞核抗原(PCNA)mRNA和雌激素受体(ER)的表达,以及类固醇急性调节蛋白(StAR)、细胞色素P450胆固醇侧链切割酶(P450scc)和3β-羟基类固醇脱氢酶(3β-HSD)mRNA的表达。免疫印迹法检测PCNA、StAR和CYP11A1蛋白表达水平。结果表明,槲皮素处理颗粒细胞可显著增强细胞活力和增殖,减少细胞凋亡,促进PCNA基因和蛋白水平的表达。槲皮素处理后,黄体酮分泌水平显著增加,用蛋白质印迹法检测StAR和CYP11A1的表达水平也显著增加。ERα的mRNA表达水平在100 ng/ml和1000 ng/ml槲皮素处理组ERβmRNA表达水平无显著差异。
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引用次数: 0
Holo-analysis of the effects of xylo-oligosaccharides on broiler chicken performance. 低聚木糖对肉鸡生产性能影响的全息分析。
IF 2 3区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-02-01 Epub Date: 2024-01-31 DOI: 10.1080/00071668.2023.2280963
N K Morgan, E Kim, G González-Ortiz

1. Xylo-oligosaccharides (XOS) stimulate proliferation of beneficial bacteria in the gastrointestinal tract of broiler chickens. This results in enhanced utilisation of dietary non-starch polysaccharides and increased production of valuable short-chain fatty acids. However, these positive effects do not always translate into improved bird productive performance, with inconsistent performance responses observed between bird trials.2. A holo-analysis was conducted to determine the effects of supplementing XOS into broiler diets on bird feed intake, body weight gain, feed conversion and mortality. This was done by comparing the XOS supplemented treatment to the control treatment. A total of 53 studies which met the criteria for inclusion were used in the analysis.3. The results showed that XOS had a notable positive impact on bird mortality; XOS reduced mortality by 0.69% for every 1% increment in the control group. XOS supplementation induced a positive effect on the feed conversion ratio (FCR). However, the efficacy of XOS at improving FCR was dependent on the efficiency of the control group (performance of the flock), and the concentration of total arabinoxylan, protein and phytase in the diet. There were insufficient data points to predict the effect of XOS on body weight and feed intake.4. In conclusion, the holo-analysis revealed that supplementing XOS to broiler chicken diets reduces bird mortality. XOS can also improve FCR, but the scale of response is dependent on the diet composition and control flock performance. Additional studies are required to confirm the effects of XOS on body weight and feed intake.

低聚木糖(XOS)可促进肉鸡胃肠道有益菌的增殖。这提高了膳食中非淀粉多糖的利用率,增加了有价值的短链脂肪酸的产量。然而,这些积极的影响并不总是转化为提高鸟类的生产性能,在不同的鸟类试验中观察到的性能反应不一致。本试验旨在研究肉仔鸡饲粮中添加XOS对禽类采食量、增重、饲料转化率和死亡率的影响。这是通过比较XOS补充处理和对照处理来完成的。共有53项符合纳入标准的研究被纳入分析。结果表明:XOS对鸟类死亡率有显著的正向影响;在对照组中,XOS每增加1%,死亡率降低0.69%。添加XOS对饲料系数(FCR)有积极影响。然而,XOS提高FCR的效果取决于对照组的效率(鸡群的生产性能)以及饲粮中总阿拉伯木聚糖、蛋白质和植酸酶的浓度。目前还没有足够的数据来预测XOS对体重和采食量的影响。综上所述,整体分析表明,在肉鸡饲粮中添加XOS可降低禽类死亡率。XOS也能提高饲料转化率,但其作用程度取决于饲粮组成和对照鸡的生产性能。需要进一步的研究来证实XOS对体重和采食量的影响。
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引用次数: 0
Genome-wide association study identifies multiple loci influencing duck serum biochemical indicators in the laying period. 全基因组关联研究发现影响蛋鸭血清生化指标的多个基因位点
IF 2 3区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-02-01 Epub Date: 2024-01-29 DOI: 10.1080/00071668.2023.2272982
J Qi, H Liu, Z Zhou, Y Jiang, W Fan, J Hu, J Li, Z Guo, M Xie, W Huang, Q Zhang, S Hou

1. Laying performance is an important economic trait in poultry. The blood is essential in transporting nutrients to the yolk and albumen and is necessary for egg formation.2. This study calculated the phenotypic relationships of duck egg quality, egg production efficiency and 22 serum parameters in the egg-laying stage. Using a variety of methodologies, a genome-wide association study (GWAS) was carried out to uncover the genetic foundations of the 22 serum biochemical markers of laying ducks.3. Spearman correlation coefficients between the egg production (226-329 per day) and the serum parameters were all weak, being less than 0.3. This analysis was done on 22 serum parameters, with total protein (TP), total triglycerides (TG), calcium (Ca) and phosphorous (P) having the highest correlation coefficients (r = 0.56-0.88). The coefficients for blood markers, such as total cholesterol (CHOL), total bilirubin (TBIL), low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C) varied from 0.70-0.94.4. Based on single-marker single-trait genome-wide analyses by a mixed linear model program of EMMAX, nine candidate genes were associated with enzyme traits (AST/ALT aspartate transaminase/glutamic-pyruvic transaminase, creatine kinase) and 19 candidate genes were associated with metabolism and protein-related serum parameters (glucose, total bile acid, uric acid (UA), albumin (ALB).5. The mvLMM (multivariate linear mixed model) of GEMMA software was used to carry out multiple trait integrated GWAS. Two candidate genes were found in the TP-TG-CA-P analysis and seven candidate genes in the CHOL_LDL-C_HDL-C_TBIL study. There was a high genetic correlation between the two groups.

1.产蛋性能是家禽的重要经济性状。2. 本研究计算了鸭蛋质量、产蛋效率与产蛋阶段 22 项血清参数的表型关系。利用多种方法,进行了全基因组关联研究(GWAS),以揭示蛋鸭 22 项血清生化指标的遗传基础。 产蛋量(226-329 颗/天)与血清参数之间的斯皮尔曼相关系数均较弱,小于 0.3。该分析针对 22 项血清参数,其中总蛋白 (TP)、总甘油三酯 (TG)、钙 (Ca) 和磷 (P) 的相关系数最高(r = 0.56-0.88)。总胆固醇(CHOL)、总胆红素(TBIL)、低密度脂蛋白胆固醇(LDL-C)和高密度脂蛋白胆固醇(HDL-C)等血液指标的相关系数在 0.70-0.94.4 之间。根据 EMMAX 的混合线性模型程序进行的单标记单性状全基因组分析,9 个候选基因与酶性状(AST/ALT 天门冬氨酸转氨酶/谷氨酸丙酮酸转氨酶、肌酸激酶)相关,19 个候选基因与代谢和蛋白质相关血清参数(葡萄糖、总胆汁酸、尿酸(UA)、白蛋白(ALB))相关。利用 GEMMA 软件的 mvLMM(多变量线性混合模型)进行多性状整合 GWAS。在 TP-TG-CA-P 分析中发现了 2 个候选基因,在 CHOL_LDL-C_HDL-C_TBIL 研究中发现了 7 个候选基因。两组之间存在高度的遗传相关性。
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British Poultry Science
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