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The impact of dietary oregano essential oil supplementation on fatty acid composition and lipid stability in eggs stored at room temperature. 膳食中补充牛至精油对室温储存鸡蛋中脂肪酸组成和脂质稳定性的影响
IF 1.6 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2024-06-01 Epub Date: 2024-03-20 DOI: 10.1080/00071668.2024.2326886
A M Johnson, J Charre-Perales, A Todd, M Arguelles-Ramos, A A B Ali

*1. In many countries, eggs are not refrigerated and must be stored at room temperature. The objective of this study was to explore the effects of dietary oregano oil (275 mg/kg; ORE) versus an unsupplemented control diet (CON) on laying hens on the shelf life and fatty acid profile of eggs.2. Treatments were randomly distributed into 10 pens containing 27 birds each. A total of 200 eggs were collected from both groups on the same day and were stored for either 0, 10, 21 and 35 d. At each storage time, egg yolks were analysed for fatty acid profile and lipid peroxidation.3. The main indicator of lipid peroxidation, malondialdehyde (MDA), was significantly lower in ORE eggs compared to CON eggs (p = 0.001). Storage time had a significant impact on MDA concentrations (p = 0.023), with the highest found after 35 d. Significant differences were found for individual fatty acids, saturated (SFA), monounsaturated (MUFA) and polyunsaturated fatty acids (PUFA). Palmitic acid, stearic acid, oleic acid, linoleic acid and arachidonic acid were significantly lower in ORE eggs compared to CON eggs (p < 0.05). Palmitoleic acid (p = 0.002), linolenic acid (p = 0.001) and docosahexaenoic acid (DHA, p = 0.001) were significantly higher in ORE eggs.4. Storage only affected oleic, linolenic, linoleic, arachidonic and docosahexaenoic acids (p < 0.05). Total SFA, MUFA, n-6 and ratio of n-3 to n-6 (n-3:n-6) PUFA were significantly higher in CON eggs (p < 0.05). The ratio of SFA to PUFA (SFA:PUFA, p = 0.005) and total n-3 PUFA (p = 0.001) were significantly higher in ORE eggs.5. The n-3:n-6 ratio was significantly impacted by treatment (p = 0.021) and storage (p = 0.031) with no significant interaction. This ratio is important for human health indication and could lead to the development of designer eggs.

1.在许多国家,鸡蛋不能冷藏,必须在室温下储存。本研究的目的是探讨牛至油(275 毫克/千克;ORE)与未添加牛至油的对照日粮(CON)相比,对蛋鸡鸡蛋保质期和脂肪酸组成的影响。 2.将各处理随机分配到 10 个围栏中,每个围栏中有 27 只鸡。3. 与对照组相比,ORE 鸡蛋中脂质过氧化的主要指标丙二醛 (MDA) 明显降低(p = 0.001)。贮藏时间对 MDA 浓度有明显影响(p = 0.023),35 天后浓度最高。在单个脂肪酸、饱和脂肪酸(SFA)、单不饱和脂肪酸(MUFA)和多不饱和脂肪酸(PUFA)方面发现了明显差异。与 CON 鸡蛋相比,ORE 鸡蛋中的棕榈酸、硬脂酸、油酸、亚油酸和花生四烯酸显著较低(p = 0.002),ORE 鸡蛋中的亚麻酸(p = 0.001)和二十二碳六烯酸(DHA,p = 0.001)显著较高。贮藏只影响油酸、亚麻酸、亚油酸、花生四烯酸和二十二碳六烯酸(p n-6 和 n-3 与 n-6 的比例(n-3:n-6)PUFA 在 CON 鸡蛋中明显较高(p p = 0.005),而总 n-3 PUFA(p = 0.001)在 ORE 鸡蛋中明显较高)。 n-3:n-6 比例受处理(p = 0.021)和贮藏(p = 0.031)的明显影响,且无明显交互作用。这一比例对人类健康非常重要,可能导致设计鸡蛋的发展。
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引用次数: 0
Digestibility and nutritional parameters of maize ethanol coproducts and xylanase for broiler diets. 肉鸡日粮中玉米乙醇副产品和木聚糖酶的消化率和营养参数。
IF 1.6 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2024-06-01 Epub Date: 2024-05-24 DOI: 10.1080/00071668.2024.2326450
L J Posadas Mendoza, A P Silva Ton, M Sbardella, V R Cambito de Paula, V L Rothmund, C Oliveira Brito, C Kiefer, A Corassa

1. The objective of this study was to determine the nutritional and energy values of four maize distiller's dried grains with solubles (DDGS) and one maize high protein distiller's dried grains (HP-DDG) from ethanol production plants in Brazil; to evaluate the digestibility, performance, nitrogen balance and energy values for broiler chickens fed diets containing these coproducts (Experiment I); and to evaluate the effects of xylanase inclusion in diets containing maize DDGS for broilers on energy availability, digestibility, nitrogen balance and gastrointestinal morphometry (Experiment II).2. For each experiment, 180 broiler chickens aged 17 and 30 days with initial weights of 450 ± 18 g and 1228 ± 33 g, respectively, were used; the chickens were distributed into 36 metabolism cages. The experimental design consisted of complete randomised blocks, with six replications per treatment and five birds per experimental unit. The treatments consisted of a basal diet (BD) and five test diets containing maize ethanol coproducts (Experiment I) one BD and five test diets containing DDGS with inclusions of 0, 8,000, 16,000, 24,000 and 32,000 BXU/kg xylanase (Experiment II). In Experiment I, HP-DDG and DDGS2 presented higher AME and AMEn values (14.1 and 13.9 MJ/kg and 13.4 and 13.3 MJ/kg, respectively), than did the other coproducts (p < 0.05). Compared with DDGS1 and DDGS3, DDGS4 and HP-DDG had higher digestible CP values (p < 0.05). In Experiment II, the inclusion of the enzyme quadratically affected the values of digestible CP and digestible EE (p < 0.05), with the maximum values occurring with the inclusion of 18 750 and 22,170 BXU/kg of xylanase, respectively.3. The digestible NDF and digestible MM values linearly increased with the inclusion of xylanase (p < 0.05). The addition of xylanase had no effect on gastrointestinal morphometry (p > 0.05). It was concluded that the inclusion of between 18,000 and 22,000 BXU/kg of xylanase resulted in better digestible CP and digestible EE values.

1.本研究的目的是测定巴西乙醇生产厂生产的四种玉米酒糟(DDGS)和一种玉米高蛋白酒糟(HP-DDG)的营养价值和能量价值;评估肉鸡饲喂含有这些副产品的日粮的消化率、生产性能、氮平衡和能量值(实验 I);评估肉鸡饲喂含有玉米 DDGS 的日粮中添加木聚糖酶对能量供应、消化率、氮平衡和胃肠道形态测量的影响(实验 II)。每个实验分别使用 180 只 17 日龄和 30 日龄的肉鸡,初始体重分别为 450 ± 18 克和 1228 ± 33 克;这些鸡被分配到 36 个新陈代谢笼中。实验设计采用完全随机区组,每个处理有六个重复,每个实验单元有五只鸡。处理包括一种基础日粮(BD)和五种含有玉米乙醇副产品的试验日粮(实验 I),一种基础日粮和五种含有 DDGS 的试验日粮(实验 II),DDGS 的木聚糖酶含量分别为 0、8,000、16,000、24,000 和 32,000 BXU/kg。在实验 I 中,HP-DDG 和 DDGS2 的 AME 和 AMEn 值(分别为 14.1 和 13.9 MJ/kg 以及 13.4 和 13.3 MJ/kg)高于其他副产品(p p p p > 0.05)。结论是,添加 18,000 至 22,000 BXU/kg 的木聚糖酶可获得更好的可消化 CP 和可消化 EE 值。
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引用次数: 0
Relationships of quality indices with wooden breast myopathy severity in chicken breast meat under refrigerated storage. 冷藏鸡胸肉的质量指标与木胸肌病严重程度的关系
IF 1.6 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2024-06-01 Epub Date: 2024-03-11 DOI: 10.1080/00071668.2024.2316865
C Kaewkot, M-D Wu, F-J Tan

1. This study investigated the relationships of quality indices with the severity of wooden breast (WB) myopathy in chicken breast meat under refrigerated storage. The physicochemical properties, water-holding capacity (WHC), microbial quality and fatty acid profiles of normal chicken breast meat samples (NOR samples, n = 63), moderate WB (MWB, n = 63) myopathy and severe WB (SWB, n = 63) myopathy (MWB and SWB samples, respectively) were evaluated immediately after sampling and after 4 and 8 d of refrigerated storage at 4°C.2. Total collagen, fat, saturated and monounsaturated fatty acid contents, redness and pH of the SWB and MWB samples were higher than the NOR samples. The SWB samples that were stored for 8 d had poor WHC, total viable counts (TVC) of higher than 7.0log colony-forming units, total volatile basic nitrogen (TVB-N) content of greater than 15 mg/100 g and a thiobarbituric acid - reactive substance level of higher than 1 mg/kg malondialdehyde.3. No significant difference was observed in the TVB-N content and TVC of the MWB and NOR samples during storage. Polyunsaturated fatty acid content was lower in the SWB and MWB samples than in the NOR samples. The SWB samples were tougher than the MWB and NOR samples after 8 d of refrigeration.4. In conclusion, the quality of chicken breast meat with SWB myopathy degraded considerably over time; thus, such meat should not be subjected to extended refrigeration for storage.

1.本研究调查了冷藏贮藏条件下鸡胸肉质量指标与木胸肌病(WB)严重程度的关系。研究人员在采样后立即对正常鸡胸肉样品(NOR 样品,n = 63)、中度木胸肌病(MWB 样品,n = 63)和重度木胸肌病(SWB 样品,n = 63)(分别为 MWB 和 SWB 样品)的理化特性、持水量(WHC)、微生物质量和脂肪酸谱进行了评估。SWB 和 MWB 样品的总胶原蛋白、脂肪、饱和脂肪酸和单不饱和脂肪酸含量、红度和 pH 值均高于 NOR 样品。贮藏 8 天的 SWB 样品的 WHC 值较差,菌落总数(TVC)高于 7.0log 菌落总数单位,总挥发性碱基氮(TVB-N)含量高于 15 mg/100 g,硫代巴比妥酸-活性物质丙二醛含量高于 1 mg/kg。 3. 在贮藏期间,MWB 和 NOR 样品的 TVB-N 含量和 TVC 没有明显差异。SWB 和 MWB 样品的多不饱和脂肪酸含量低于 NOR 样品。冷藏 8 天后,SWB 样品比 MWB 和 NOR 样品更硬。 4. 总之,患有 SWB 肌病的鸡胸肉的质量会随着时间的推移而显著下降,因此,这类肉类不应长时间冷藏贮存。
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引用次数: 0
Cereal type and conditioning temperature altered protein and carbohydrate molecular structure, nutrient retention and performance in broilers fed pellet diets during starter and grower period. 谷物类型和调节温度改变了肉鸡初生期和生长期颗粒日粮中蛋白质和碳水化合物的分子结构、营养保留和性能。
IF 1.6 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2024-06-01 Epub Date: 2024-02-23 DOI: 10.1080/00071668.2024.2310668
A Heydari, S Khalaji, M Yari

1. Effects of cereal type and conditioning temperature (CT) on protein and carbohydrate (CHO) molecular structures, nutrient retention, carcass and blood characteristics, caecal microbial population and growth criteria of broilers fed pellet diet were evaluated for a total period of 35 d.2. In total, 336-day-old Cobb 500 broiler chicks were randomly allotted into a 2 × 2 factorial arrangement with two different cereal types (maize or wheat) processed in two different temperatures (CT; 68°C or 90°C) with seven pen replicates containing 12 birds each.3. Chicks fed the maize-based diets significantly gained higher body weight (BW) and lower feed conversion ratio (FCR) in comparison to the chicks fed wheat-based diets during the whole grow-out period (p < 0.01). Overall, the highest BW and feed intake (FI) were seen in birds fed wheat-based diets conditioned at 68°C, but the lowest FCR was observed in maize-based diet conditioned at 90°C at 7, 14 and 21 d of age (p < 0.01). However, BW was higher and FCR lower in chicks fed maize-based diets conditioned at 90°C in the grower period (28 and 35 d; p < 0.01).4. The α-helix height was higher in wheat-based starter diets in comparison to the maize-based diet (p < 0.01). Ratio of amide I to II area and total CHO peak height were increased when diets were processed at 90°C in both maize and wheat-based starter diet (p < 0.05). Increasing the CT from 68°C to 90°C reduced CHO peak 1 and 2 height by 11.6% and 3.95%, respectively, in maize-based starter diets, while increasing the CT from 68°C to 90°C reduced CHO peak 1 and 2 height by 54.3% and 57.2%, respectively, in wheat-based starter diets. In the grower diets, increasing the CT from 68°C to 90°C increased CHO peak 1 by 23% in maize-based diets, but reduced CHO peak 1 by 24.5% in wheat-based diets.5. Calcium and phosphorous retention were highest in chicks fed wheat-based diets conditioned at 90°C and lowest in chicks fed maize-based diets conditioned at 90°C (p < 0.01). Salmonella, E. coli and coliforms in the caeca reduced significantly (p < 0.05) in chicks fed wheat-based diets conditioned at 90°C on d 11 and increased with the same diet at 35 d of age compared to the chicks fed maize-based diets conditioned at both 68°C and 90°C or wheat-based diets conditioned at 68°C.6. Conditioning the wheat-based diets at 68°C improved production responses without causing any adverse effects on protein and CHO molecular structures, however increasing the conditioning temperature to 90°C impaired performance due to alteration of protein and CHO molecular structures. In contrast, conditioning of the maize-based diets at 90°C had the opposite effect, and improved production performance compared to diets conditioned at 68°C.

1.评估了谷物种类和调理温度(CT)对饲喂颗粒日粮的肉鸡蛋白质和碳水化合物(CHO)分子结构、营养保留、胴体和血液特征、盲肠微生物数量和生长标准的影响,总计 35 d2。将 336 日龄的 Cobb 500 肉鸡雏鸡随机分配到 2 × 2 因式排列中,两种不同谷物类型(玉米或小麦)在两种不同温度(CT;68°C 或 90°C)下加工,共设 7 个重复栏,每个重复栏含 12 只雏鸡。与饲喂小麦日粮的雏鸡相比,饲喂玉米日粮的雏鸡在整个生长期体重明显增加,饲料转化率(FCR)明显降低(p p p p p p 盲肠中的沙门氏菌、大肠杆菌和大肠菌群明显减少(p p p p p p
{"title":"Cereal type and conditioning temperature altered protein and carbohydrate molecular structure, nutrient retention and performance in broilers fed pellet diets during starter and grower period.","authors":"A Heydari, S Khalaji, M Yari","doi":"10.1080/00071668.2024.2310668","DOIUrl":"10.1080/00071668.2024.2310668","url":null,"abstract":"<p><p>1. Effects of cereal type and conditioning temperature (CT) on protein and carbohydrate (CHO) molecular structures, nutrient retention, carcass and blood characteristics, caecal microbial population and growth criteria of broilers fed pellet diet were evaluated for a total period of 35 d.2. In total, 336-day-old Cobb 500 broiler chicks were randomly allotted into a 2 × 2 factorial arrangement with two different cereal types (maize or wheat) processed in two different temperatures (CT; 68°C or 90°C) with seven pen replicates containing 12 birds each.3. Chicks fed the maize-based diets significantly gained higher body weight (BW) and lower feed conversion ratio (FCR) in comparison to the chicks fed wheat-based diets during the whole grow-out period (<i>p</i> < 0.01). Overall, the highest BW and feed intake (FI) were seen in birds fed wheat-based diets conditioned at 68°C, but the lowest FCR was observed in maize-based diet conditioned at 90°C at 7, 14 and 21 d of age (<i>p</i> < 0.01). However, BW was higher and FCR lower in chicks fed maize-based diets conditioned at 90°C in the grower period (28 and 35 d; <i>p</i> < 0.01).4. The α-helix height was higher in wheat-based starter diets in comparison to the maize-based diet (<i>p</i> < 0.01). Ratio of amide I to II area and total CHO peak height were increased when diets were processed at 90°C in both maize and wheat-based starter diet (<i>p</i> < 0.05). Increasing the CT from 68°C to 90°C reduced CHO peak 1 and 2 height by 11.6% and 3.95%, respectively, in maize-based starter diets, while increasing the CT from 68°C to 90°C reduced CHO peak 1 and 2 height by 54.3% and 57.2%, respectively, in wheat-based starter diets. In the grower diets, increasing the CT from 68°C to 90°C increased CHO peak 1 by 23% in maize-based diets, but reduced CHO peak 1 by 24.5% in wheat-based diets.5. Calcium and phosphorous retention were highest in chicks fed wheat-based diets conditioned at 90°C and lowest in chicks fed maize-based diets conditioned at 90°C (<i>p</i> < 0.01). <i>Salmonella</i>, <i>E. coli</i> and coliforms in the caeca reduced significantly (<i>p</i> < 0.05) in chicks fed wheat-based diets conditioned at 90°C on d 11 and increased with the same diet at 35 d of age compared to the chicks fed maize-based diets conditioned at both 68°C and 90°C or wheat-based diets conditioned at 68°C.6. Conditioning the wheat-based diets at 68°C improved production responses without causing any adverse effects on protein and CHO molecular structures, however increasing the conditioning temperature to 90°C impaired performance due to alteration of protein and CHO molecular structures. In contrast, conditioning of the maize-based diets at 90°C had the opposite effect, and improved production performance compared to diets conditioned at 68°C.</p>","PeriodicalId":9322,"journal":{"name":"British Poultry Science","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139939733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Bone characteristics, pre-caecal phytate degradation, mineral digestibility and tissue expression were marginally affected by zinc level and source in phytase-supplemented diets in 21-day-old broiler chickens. 21 日龄肉鸡的骨骼特征、盲肠前植酸降解、矿物质消化率和组织表达受植酸酶补充日粮中锌水平和来源的影响较小。
IF 1.6 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2024-06-01 Epub Date: 2024-02-23 DOI: 10.1080/00071668.2024.2311290
H Philippi, V Sommerfeld, A Monteiro, M Rodehutscord, O A Olukosi

1. This study determined the effect of dietary Zn concentration and source in phytase-supplemented diets on bone mineralisation, gastrointestinal phytate breakdown, mRNA-level gene expression (in jejunum, liver and Pectoralis major muscle) and growth performance in broiler chickens.2. Male Cobb 500 broilers were housed in floor pens (d 0-d 21) to test seven treatments with six replicate pens (12 birds per pen). Diets were arranged in a 2 × 3 + 1-factorial arrangement. The experimental factors were Zn source (Zn-oxide (ZnO) or Zn-glycinate (ZnGly) and Zn supplementation level (10, 30 or 50 mg/kg of diet). A maize-soybean meal-based diet without supplementation and formulated to contain 28 mg Zn/kg (analysed to be 35 mg Zn/kg), served as a control.3. Zinc source and level did not influence (p > 0.05) bone ash concentration and quantity or mineral concentrations in bone ash. Tibia thickness was greater in the treatment ZnO10 than in the treatments ZnO30 and ZnGly50 (Zn level × Zn source: p = 0.036), but width and breaking strength were not affected.4. Pre-caecal P digestibility and concentrations of phytate breakdown products in the ileum, except for InsP5, were not affected by Zn source or level. Only the expression of EIF4EBP1 (eukaryotic translation initiation factor 4E-binding protein 1) and FBXO32 (F-box only protein 32) in Pectoralis major muscle was affected by source, where expression was increased in ZnO compared to ZnGly diets (p < 0.05).5. In conclusion, Zn level and source did not affect gastrointestinal phytate degradation and bone mineralisation in phytase-supplemented diets. The intrinsic Zn concentration appeared to be sufficient for maximum bone Zn deposition under the conditions of the present study but requires validation in longer-term trials.

1.本研究确定了补充植酸酶的日粮中锌的浓度和来源对肉鸡骨骼矿化、胃肠道植酸分解、mRNA 水平基因表达(空肠、肝脏和胸大肌)和生长性能的影响。 雄性 Cobb 500 肉鸡饲养在落地式鸡笼中(0-21 日龄),以六个重复鸡笼(每个鸡笼 12 只鸡)对七个处理进行试验。日粮采用 2 × 3 + 1 的因子排列。试验因子为锌来源(氧化锌(ZnO)或甘氨酸锌(ZnGly))和锌补充水平(10、30 或 50 mg/kg 日粮)。3. 锌的来源和水平不影响骨灰浓度和数量或骨灰中的矿物质浓度(p > 0.05)。ZnO10 处理的胫骨厚度大于 ZnO30 和 ZnGly50 处理的胫骨厚度(锌水平 × 锌来源:p = 0.036),但宽度和断裂强度不受影响。 4.除 InsP5 外,盲肠前 P 消化率和回肠中植酸分解产物的浓度不受锌来源或锌水平的影响。只有 EIF4EBP1(真核翻译起始因子 4E 结合蛋白 1)和 FBXO32(仅 F-box 蛋白 32)在胸大肌中的表达受到来源的影响,与 ZnGly 日粮相比,ZnO 日粮中的表达增加了(p
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引用次数: 0
Effects of ferritin heavy chain on oxidative stress, cell proliferation and apoptosis in geese follicular granulosa cells. 铁蛋白重链对鹅卵泡颗粒细胞氧化应激、细胞增殖和凋亡的影响
IF 1.6 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2024-06-01 Epub Date: 2024-03-08 DOI: 10.1080/00071668.2024.2315086
D Jiang, X An, Q Xu, G Mo, W Ling, C Ji, Z Wang, X Wang, Q Sun, B Kang

1. The ferritin heavy chain (FHC) has a vital impact on follicular development in geese, due to its ability to regulate apoptosis of granulosa cells (GCs) and follicular atresia. However, its specific regulatory mechanisms remain unclear. The present study characterised how FHC regulates oxidative stress, cell proliferation and apoptosis in goose GCs by interfering with and overexpressing the FHC gene.2. After 72 h of interference with FHC expression, the activity of GCs decreased remarkably (p < 0.05), reactive oxygen species (ROS) levels and the expression levels of antioxidant enzyme genes catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) increased significantly (p < 0.05). The overexpression of FHC for 72 h was found to significantly reduce the expression of CAT and SOD genes (p < 0.05).3. Interfering with FHC expression revealed that the expression levels of the cell proliferation gene Aurora kinase A (AURORA-A) were significantly decreased (p < 0.05), while the expression levels of the apoptosis genes B-cell lymphoma-2 (BCL-2) and cysteine aspartate-specific protease 8 (CASPASE 8) increased (p < 0.05). Further research has shown that, when interfering with FHC expression for 72 h, apoptosis rate increased by 1.19-fold (p < 0.05), but the current data showed a lower apoptosis rate after FHC overexpression by 59.41%, 63.39%, and 52.31% at three different treatment times (p < 0.05).4. In conclusion, FHC improved the antioxidant capacity of GCs, promotes GCs proliferation, and inhibits GCs apoptosis of ovarian follicles in Sichuan white geese.

1.铁蛋白重链(FHC)能调节颗粒细胞(GCs)凋亡和卵泡闭锁,因此对鹅的卵泡发育有重要影响。然而,其具体的调控机制仍不清楚。本研究通过干扰和过表达 FHC 基因,描述了 FHC 如何调节鹅 GCs 的氧化应激、细胞增殖和凋亡。.干扰 FHC 表达 72 小时后,鹅胆细胞的活性显著下降(p CAT)、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)显著增加(p FHC 表达 72 h,CAT 和 SOD 基因表达显著降低(p FHC 表达显示细胞增殖基因极光激酶 A(AURORA-A)表达水平显著降低(p BCL-2),半胱氨酸天冬氨酸特异性蛋白酶 8(CASPASE 8)表达水平增加(p FHC 表达 72 h,细胞凋亡率增加 1.19倍(p FHC在三个不同的处理时间分别过表达59.41%、63.39%和52.31%(P
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引用次数: 0
Limosilactobacillus reuteri peptidoglycan alleviates aflatoxin B1-induced toxicity through adsorbing toxins and improving growth, antioxidant status, immunity and liver pathological changes in chicks. 通过吸附毒素并改善雏鸡的生长、抗氧化状态、免疫力和肝脏病理变化,Limosilactobacillus reuteri肽聚糖可减轻黄曲霉毒素B1诱导的毒性。
IF 1.6 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2024-06-01 Epub Date: 2024-03-11 DOI: 10.1080/00071668.2024.2316228
F H Zhu, X Y Chen, L L Hou, J H Dong, H W Liu, L Q Zhu, F Chen

1. The objective of this study was to investigate the protective effects of a peptidoglycan produced by Limosilactobacillus reuteri against aflatoxin B1 (AFB1) induced toxicity in vitro and in vivo in broiler chicks.2. Toxin adsorption experiments were carried out firstly in vitro. These experiments indicated that the absorption efficiency of the peptidoglycan for AFB1 was 64.3-75.9%.3. In the in vivo experiments, Hy-Line Brown chicks were fed a diet containing AFB1 at 71.43 µg/kg with and without peptidoglycan supplementation at concentrations of 100, 200, or 300 g/kg feed from 0-42 d of age.4. The peptidoglycan supplementation in AFB1-contaminated diets resulted in significant improvements in terms of average daily gain, feed intake, feed conversion ratio, white blood cell count, haemoglobin content, glutathione peroxidase activity, immunoglobulin (Ig) A, IgG, IgM and Newcastle disease virus antibody titres (p < 0.05) and diminished liver steatosis.5. In conclusion, peptidoglycan supplementation alleviated AFB1-induced toxicity through adsorbing toxins and improving growth performance, antioxidant ability, immunity and liver pathological changes in chicks. The optimal supplemental dose was 200 mg/kg in feed.

1.本研究的目的是调查由Limosilactobacillus reuteri产生的肽聚糖在体外和体内对肉鸡黄曲霉毒素B1(AFB1)引起的毒性的保护作用。 2. 首先在体外进行了毒素吸附实验。这些实验表明,肽聚糖对 AFB1 的吸收率为 64.3%-75.9%。在体内实验中,从 0-42 日龄开始,用含 AFB1(71.43 微克/千克)的日粮喂养 Hy-Line 棕色小鸡,添加或不添加肽聚糖,浓度分别为 100、200 或 300 克/千克饲料。在受 AFB1 污染的日粮中添加肽聚糖可显著提高雏鸡的平均日增重、采食量、饲料转化率、白细胞计数、血红蛋白含量、谷胱甘肽过氧化物酶活性、免疫球蛋白 (Ig)A、IgG、IgM 和新城疫病毒抗体滴度(p 1 通过吸附毒素诱发毒性,改善雏鸡的生长性能、抗氧化能力、免疫力和肝脏病理变化)。最佳补充剂量为 200 毫克/千克饲料。
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引用次数: 0
Identification of potential candidate genes for the Huoyan trait in developing Wulong goose embryos by transcriptomic analysis. 通过转录组分析鉴定发育中的乌龙鹅胚胎后燕性状的潜在候选基因。
IF 1.6 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2024-06-01 Epub Date: 2024-05-10 DOI: 10.1080/00071668.2024.2328686
Y Xiao, J Liu, P Ren, X Zhou, S Zhang, Z Li, J Gong, R Li, M Zhu

1. The Wulong goose is a Chinese breed and a source of high-quality meat and eggs. A characteristic of the Wulong goose is that a proportion of the birds do not have eyelids, known as the Huoyon trait.2. Wulong geese exhibiting the Huoyan trait at embryonic stages of 9 days (E9), 12 days (E12) and 14 days (E14) were selected alongside those with normal eyelids for comprehensive transcriptome sequencing. Differentially expressed gene (DEG) and functional enrichment analyses were performed and finally, eight DEG were chosen to verify the accuracy of qPCR sequencing.3. Overall, 466, 962 and 550 DEG were obtained from the three control groups, D9 vs. N9, D12 vs. N12 and D14 vs. N14, respectively, by differential analysis (p < 0.05). CDKN1C, CRH, CROCC and TYSND1 were significantly expressed in the three groups. Enrichment analysis revealed the enrichment of CROCC and TYSND1 in pathways of cell cycle process, endocytosis, microtubule-based process, microtubule organising centre organisation, protein processing and protein maturation. CDKN1C and CRH were enriched in the cell cycle and cAMP signalling pathway.4. Some collagen family genes were detected among the DEGs, including COL3A1, COL4A5, COL4A2 and COL4A1. FREM1 and FREM2 genes were detected in both Huoyan and normal eyelids. There was a significant difference (p < 0.01) in FREM1 expression between ED9 and ED14 in female embryos, but this difference was not observed in male embryos.

1.乌龙鹅是中国的一个品种,也是优质肉蛋的来源。武隆鹅的一个特点是有一部分鹅没有眼睑,这被称为 "火炎性状"。2. 挑选出在 9 天(E9)、12 天(E12)和 14 天(E14)胚胎期表现出 "火炎 "性状的乌龙鹅,与眼睑正常的乌龙鹅一起进行全面的转录组测序。通过差异分析(p CDKN1C、CRH、CROCC 和 TYSND1 在三个对照组中均有显著表达),三个对照组的差异表达基因分别为 466、962 和 550 个。富集分析显示,CROCC 和 TYSND1 在细胞周期过程、内吞、基于微管的过程、微管组织中心组织、蛋白质加工和蛋白质成熟等通路中富集。CDKN1C和CRH富集在细胞周期和cAMP信号通路中。 在DEGs中发现了一些胶原家族基因,包括COL3A1、COL4A5、COL4A2和COL4A1。在霍燕和正常眼睑中都检测到了 FREM1 和 FREM2 基因。霍燕和正常眼睑中的 FREM1 和 FREM2 基因有明显差异(p
{"title":"Identification of potential candidate genes for the Huoyan trait in developing Wulong goose embryos by transcriptomic analysis.","authors":"Y Xiao, J Liu, P Ren, X Zhou, S Zhang, Z Li, J Gong, R Li, M Zhu","doi":"10.1080/00071668.2024.2328686","DOIUrl":"10.1080/00071668.2024.2328686","url":null,"abstract":"<p><p>1. The Wulong goose is a Chinese breed and a source of high-quality meat and eggs. A characteristic of the Wulong goose is that a proportion of the birds do not have eyelids, known as the Huoyon trait.2. Wulong geese exhibiting the Huoyan trait at embryonic stages of 9 days (E9), 12 days (E12) and 14 days (E14) were selected alongside those with normal eyelids for comprehensive transcriptome sequencing. Differentially expressed gene (DEG) and functional enrichment analyses were performed and finally, eight DEG were chosen to verify the accuracy of qPCR sequencing.3. Overall, 466, 962 and 550 DEG were obtained from the three control groups, D9 <i>vs</i>. N9, D12 <i>vs</i>. N12 and D14 <i>vs</i>. N14, respectively, by differential analysis (<i>p</i> < 0.05). <i>CDKN1C</i>, <i>CRH</i>, <i>CROCC</i> and <i>TYSND1</i> were significantly expressed in the three groups. Enrichment analysis revealed the enrichment of <i>CROCC</i> and <i>TYSND1</i> in pathways of cell cycle process, endocytosis, microtubule-based process, microtubule organising centre organisation, protein processing and protein maturation. <i>CDKN1C</i> and <i>CRH</i> were enriched in the cell cycle and cAMP signalling pathway.4. Some collagen family genes were detected among the DEGs, including <i>COL3A1</i>, <i>COL4A5</i>, <i>COL4A2</i> and <i>COL4A1</i>. <i>FREM1</i> and <i>FREM2</i> genes were detected in both Huoyan and normal eyelids. There was a significant difference (<i>p</i> < 0.01) in FREM1 expression between ED9 and ED14 in female embryos, but this difference was not observed in male embryos.</p>","PeriodicalId":9322,"journal":{"name":"British Poultry Science","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140896783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluation of the potential growth and body composition of the Cobb 700 genotype. 评估柯布 700 基因型的生长潜力和身体组成。
IF 1.6 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2024-06-01 Epub Date: 2024-05-24 DOI: 10.1080/00071668.2024.2328680
R M Gous, H Walters, S J Rochell, G C Emmans

1. The potential growth of the chemical and physical components of males and females of the Cobb 700 strain was measured from hatch to 15 weeks of age.2. A four-phase ad libitum feeding programme was used to feed 200 chicks of each sex. All birds were weighed weekly. Ten birds per sex were sampled at 0, 7, 14, 28, 42, 56, 70, 84 and 105 d of age. They were weighed before and after plucking to determine the weight of feathers. Physical parts were measured on defeathered birds, whereafter these components were combined, minced, freeze dried to measure water content, and then analysed for protein, lipid and ash content.3. Mature body weights of males and females averaged 8.38 and 6.94 kg, respectively, mature body protein weights averaged 1.48 and 1.19 kg and mature body lipid contents averaged 1.08 and 1.54 kg, respectively.4. Rates of maturing of the empty feather-free body weights of males and females averaged 0.0417 and 0.0402/d, respectively. All chemical and physical components within a sex, other than feathers, had the same rate of maturing. The rate of maturing of feathers, calculated by iteration, in males was lower than in females (0.0324 vs. 0.0357/d) and the mature weight was higher (435 vs. 372 g).5. The ratios of the chemical components to feather-free body protein at maturity for males and females were, for water, 3.80 and 3.34; for lipid, 0.73 and 1.29; and for ash, 0.13 and 0.19, respectively. Separate equations were required for males and females to describe the allometric relationship between lipid and protein in the feather-free body.6. Mature body weights of broilers in this trial were considerably higher than those measured using the same protocol 28 years ago, whereas rates of maturing have remained the same.

1.2. 采用四阶段自由采食方案,每阶段饲喂 200 只雌雄雏鸡。所有雏鸟每周称重一次。在 0、7、14、28、42、56、70、84 和 105 日龄时,每种性别各抽取 10 只雏鸡。在拔毛前后称重,以确定羽毛的重量。测量羽毛的物理部分,然后将这些部分合并、剁碎、冷冻干燥以测量含水量,然后分析蛋白质、脂质和灰分含量。雄鸟和雌鸟的成熟体重平均分别为 8.38 千克和 6.94 千克,成熟体内蛋白质重量平均分别为 1.48 千克和 1.19 千克,成熟体内脂质含量平均分别为 1.08 千克和 1.54 千克。 雄鸟和雌鸟的空无羽体重成熟速度平均分别为 0.0417 和 0.0402/天。除羽毛外,性别内所有化学和物理成分的成熟率相同。通过迭代计算,雄性羽毛的成熟率低于雌性(0.0324 对 0.0357/d),成熟体重高于雌性(435 对 372 g)。 雄性和雌性羽毛成熟时的化学成分与无羽体蛋白质的比率分别为:水分 3.80 对 3.34;脂质 0.73 对 1.29;灰分 0.13 对 0.19。雄鸡和雌鸡需要使用不同的方程来描述无羽体脂质和蛋白质之间的异速关系。 6. 本次试验中肉鸡的成熟体重大大高于 28 年前使用相同方案测定的体重,而成熟率却保持不变。
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引用次数: 0
Comprehensive analysis of changes in expression of lncRNA, microRNA and mRNA in liver tissues of chickens with high or low abdominal fat deposition. 全面分析腹部脂肪沉积高或低的鸡肝脏组织中 lncRNA、microRNA 和 mRNA 的表达变化。
IF 1.6 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2024-06-01 Epub Date: 2024-05-29 DOI: 10.1080/00071668.2024.2319779
S Yu, G Wang, X Shen, J Chen, J Liao, Y Yang, G Aikebai

1. The liver of chickens is a dominant lipid biosynthetic tissue and plays a vital role in fat deposition, particularly in the abdomen. To determine the molecular mechanisms involved in its lipid metabolism, the livers of chickens with high (H) or low (L) abdominal fat content were sampled and sequencing on long non-coding RNA (lncRNA), messenger RNA (mRNA) and small RNA (microRNA) was performed.2. In total, 351 expressed protein-coding genes for long non-coding RNA (DEL; 201 upregulated and 150 downregulated), 400 differentially expressed genes (DEG; 223 upregulated and 177 downregulated) and 10 differentially expressed miRNA (DEM; four upregulated and six downregulated) were identified between the two groups. Multiple potential signalling pathways related to lipogenesis and lipid metabolism were identified via pathway enrichment analysis. In addition, 173 lncRNA - miRNA - mRNA interaction regulatory networks were identified, including 30 lncRNA, 27 mRNA and seven miRNA.3. These networks may help regulate lipid metabolism and fat deposition. Five promising candidate genes and two lncRNA may play important roles in the regulation of adipogenesis and lipid metabolism in chickens.

1.鸡的肝脏是主要的脂质生物合成组织,在脂肪沉积,尤其是腹部脂肪沉积中起着至关重要的作用。为了确定鸡肝脂质代谢的分子机制,对腹部脂肪含量高(H)或低(L)的鸡肝进行了采样,并对长非编码 RNA(lncRNA)、信使 RNA(mRNA)和小 RNA(microRNA)进行了测序。两组之间共鉴定出 351 个长非编码 RNA 蛋白编码基因(DEL:201 个上调,150 个下调)、400 个差异表达基因(DEG:223 个上调,177 个下调)和 10 个差异表达 miRNA(DEM:4 个上调,6 个下调)。通过通路富集分析,确定了与脂肪生成和脂质代谢相关的多个潜在信号通路。此外,还发现了 173 个 lncRNA - miRNA - mRNA 相互调控网络,包括 30 个 lncRNA、27 个 mRNA 和 7 个 miRNA。这些网络可能有助于调节脂质代谢和脂肪沉积。5个有希望的候选基因和2个lncRNA可能在鸡的脂肪生成和脂质代谢调控中发挥重要作用。
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引用次数: 0
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British Poultry Science
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