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Mannan oligosaccharide as an alternative to infeed antibiotics to improve growth performance of broilers: a meta-analysis. 用甘露寡糖替代饲料抗生素提高肉鸡生长性能:一项荟萃分析。
IF 1.6 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2024-08-30 DOI: 10.1080/00071668.2024.2391764
B R Polidoro, M J K de Oliveira, F D S C Braga, G D V Polycarpo

1. The purpose of this meta-analysis was to evaluate the effect of mannan oligosaccharide (MOS) as an alternative to antibiotic growth promoters (AGP) on feed intake (FI), body weight gain (BWG), and feed conversion ratio (FCR) of broilers.2. Data from 75,594 broilers were extracted from 17 articles (19 trials) published between January 2010 and March 2023. The main criteria for the publication selection were as follows, at least three treatments applied (negative control group without MOS or AGP versus MOS or AGP supplementation), presence of performance results, and intra-experimental variation associated with the mean of response (such as standard error). Treatments were classified as control, MOS, or AGP, and adjusted means of treatment were compared. Additionally, the average daily gain (ADG) and average daily feed intake (ADFI) of each type of supplementation were calculated relative (Δ) to the control group (ΔADFI and ΔADG) and expressed as a percentage of the difference.3. Broilers receiving a diet supplemented with MOS had a 3.7% better BWG and 3% better FCR compared to the control diet (P < 0.001), but these variables were similar to the group receiving AGP supplementation. No significant difference was detected in FI among treatments (P > 0.050). The relationship between ΔADG and ΔADFI was linear for the MOS and AGP-supplemented group (P < 0.050). The ΔADG of broilers fed diets supplemented with MOS or AGP was 6.4% and 4.54% when ΔADFI was zero, respectively. The ΔADG of MOS increased by 0.58% for every 1% of increasing observed in ΔADFI. The corresponding value for the increased ΔADG for the AGP group was 0.69%.4. The results of this meta-analysis indicated that MOS supplementation is effective in increasing BWG and reducing FCR, similar to broilers fed a diet supplemented with AGP. Therefore, MOS is a safe and sustainable alternative for AGP-free poultry production.

1.这项荟萃分析的目的是评估甘露寡糖(MOS)作为抗生素生长促进剂(AGP)的替代品对肉鸡采食量(FI)、体重增加(BWG)和饲料转化率(FCR)的影响。 从 2010 年 1 月至 2023 年 3 月间发表的 17 篇文章(19 项试验)中提取了 75 594 只肉鸡的数据。选择文章的主要标准如下:至少采用三种处理方法(不添加 MOS 或 AGP 的阴性对照组与添加 MOS 或 AGP 的对照组)、存在性能结果以及与反应平均值相关的试验内变异(如标准误差)。处理分为对照组、MOS 组或 AGP 组,并对调整后的处理平均值进行比较。此外,还计算了相对于对照组(ΔADFI 和 ΔADG)的每种补充日粮的平均日增重(ADG)和平均日采食量(ADFI),并以差值的百分比表示。 与对照组日粮相比,接受 MOS 补充日粮的肉鸡的 BWG 和 FCR 分别提高了 3.7% 和 3%(P P > 0.050)。在添加 MOS 和 AGP 的组别中,ΔADG 和 ΔADFI 之间呈线性关系(P
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引用次数: 0
Effects of intermittent lighting program and light colour on ocular health variables as welfare indicators in broiler chickens. 间歇性光照程序和光色对作为福利指标的肉鸡眼部健康变量的影响。
IF 1.6 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2024-08-23 DOI: 10.1080/00071668.2024.2383911
E Derelі Fіdan, R Yaygıngül, M Kaya

1. The objective of the present study was to examine the effect of lighting programs and light colour on ocular health variables as welfare indicators in Ross 308 broilers.2. A total of 384, male, one-d-old broiler chickens (Ross 308) were placed in a completely randomised design with a 2 × 2 factorial arrangement of lighting program (continuous or intermittent) and light colour (white and green LED light). Ross 308 broilers under restricted lighting had 18 h of light (18 L:6D), while those under intermittent lighting had cycles of 17 L:3D:1 L:3D throughout the experimental period, which lasted 42 d.3. At the end of the experiment, all eyes of birds (n = 96 birds) underwent a complete ophthalmic examination, which included the Schirmer tear test I, intraocular pressure and eye dimensions. In addition, 32 broilers (eight birds per trial groups) aged 42 d underwent ophthalmic examination to include assessment of ocular ultrasound biometry.4. Light colour had a significant influence on the mean intraocular pressure (p < 0.001). The Ross 308 broilers kept with intermittent lighting had lower eye weights (2.29 g; p < 0.05), palpebral fissure length (14.39 mm; p < 0.01), eye dorsoventral diameter (17.46 mm; p < 0.05), anteroposterior size (13.70 mm; p < 0.01) and corneal dorsoventral diameter (7.81 mm; p < 0.05) compared to those reared under restricted lighting.5. In conclusion, these values for Ross 308 broilers may be applied in poultry ophthalmology to detect early eye disease symptoms and to help the diagnosis of tear disorders that could cause economic losses and welfare issues. Intermittent lighting and green LED light may help reduce eye health problems thus contributing to improved welfare in broilers.

1.本研究的目的是考察光照程序和光色对作为福利指标的罗斯 308 肉鸡眼部健康变量的影响。 2. 将 384 只一龄雄性肉鸡(罗斯 308)置于完全随机设计的光照程序(连续或间歇)和光色(白色和绿色 LED 灯)的 2 × 2 因子排列中。限制光照下的罗斯 308 肉鸡有 18 小时的光照(18 L:6D),而间歇光照下的肉鸡在整个实验期间的光照周期为 17 L:3D:1L:3D,实验持续 42 d.3 实验结束时,对所有鸡只的眼睛(n = 96 只)进行了全面的眼科检查,包括施尔默泪液测试 I、眼压和眼球尺寸。此外,32 只 42 d 大的肉鸡(每个试验组 8 只)接受了眼科检查,包括眼部超声波生物测量评估。 光色对平均眼压有显著影响(p p p p p p
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引用次数: 0
Heavy metals accumulation in the meat, kidney and liver of cattle, broilers and goats sold in Quetta, northwestern city of Balochistan, Pakistan. 巴基斯坦俾路支省西北部城市奎达出售的牛、肉鸡和山羊的肉、肾脏和肝脏中重金属的积累情况。
IF 1.6 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2024-08-16 DOI: 10.1080/00071668.2024.2377743
A Mushtaq, A Sajjad, T Ismail, O Ali

1. It was assumed that the concentrations of arsenic (As), chromium (Cr), cobalt (Co), lead (Pb), manganese (Mn) and mercury (Hg) in the meat, kidney and liver of cattle, broilers and goats within Quetta city potentially exceeded the permissible limits for heavy metal content. Risk assessment of these heavy metals on human health were estimated based on daily intake (EDI), target hazard quotient (THQ) and hazard index (HI).2. Samples of the meat, liver and kidney of cattle, broilers and goats were collected from butcher shops and slaughterhouses in 20 different areas of Quetta city. Overall, 180 samples were analysed, each for six heavy metals. After acid digestion of meat samples, heavy metals analysis was carried out via atomic absorption spectroscopy.3. The results varied in levels depending upon the species and organ type. The average concentrations of As, Pb and Hg were within the established reference limits of the Australia New Zealand Food Authority (ANZFA 2015) and the European Commission (EC, 2006), although some samples exceeded these limits. Compared with the kidney and meat, the liver was the primary tissue for Cr, Pb and Hg bioaccumulation. Metals concentration were mostly within the normal range in samples from cattle and goats, while the Mn concentration was elevated in broilers.4. The estimated daily intake (EDI) revealed that the intake of As, Co, Pb and Hg was mainly derived from goat and cattle products, whereas broiler samples were more contaminated with Cr and Mn. The EDIs of Cr and Hg in adults exceeded the oral reference dose, referring to potential adverse effects. The EDIs for Co and Mn were low, suggesting that additional intake sources were necessary. Except for As, none of the determined elements had a target hazard quotient (THQ) above 1.

1.假定奎达市内的牛、肉鸡和山羊的肉、肾和肝脏中砷(As)、铬(Cr)、钴(Co)、铅(Pb)、锰(Mn)和汞(Hg)的浓度可能超过重金属含量的允许限值。根据每日摄入量(EDI)、目标危害商数(THQ)和危害指数(HI)对这些重金属对人体健康的风险评估进行了估算。 2. 从奎达市 20 个不同地区的肉店和屠宰场收集了牛、肉鸡和山羊的肉类、肝脏和肾脏样本。总共分析了 180 个样本,每个样本分析六种重金属。在对肉类样本进行酸性消化后,通过原子吸收光谱进行重金属分析。 结果因肉类种类和器官类型而异。砷、铅和汞的平均浓度在澳大利亚-新西兰食品管理局(ANZFA,2015 年)和欧盟委员会(EC,2006 年)规定的参考限值范围内,但有些样本超过了这些限值。与肾脏和肉类相比,肝脏是铬、铅和汞生物累积的主要组织。4. 估计日摄入量(EDI)显示,砷、钴、铅和汞的摄入量主要来自山羊和牛的产品,而肉鸡样品中的铬和锰污染更严重。成人的铬和汞的每日允许摄入量超过了口服参考剂量,可能会产生不良影响。钴和锰的 EDI 值较低,表明有必要增加摄入来源。除砷外,其他已确定元素的目标危害商数(THQ)均未超过 1。
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引用次数: 0
Food safety control in poultry industry: prevalence and antimicrobial susceptibility of Escherichia coli isolated from raw chicken and the potential use of Origanum essential oils as alternative to antibiotics. 家禽业的食品安全控制:从生鸡肉中分离出的大肠埃希氏菌的流行率和抗菌药敏感性,以及牛至精油作为抗生素替代品的潜在用途。
IF 1.6 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2024-08-01 Epub Date: 2024-07-12 DOI: 10.1080/00071668.2024.2346307
C Abou-Jaoudeh, J Khalil, E El-Hayek, R Abou-Khalil

1. The extensive use of antimicrobials in poultry production may contribute to the emergence of resistant bacteria. This study was conducted to determine the prevalence and resistance of different E. coli strains isolated from raw chicken meat and to investigate the possibility to use Lebanese native oregano essential oils as alternatives.2. In total, 250 chickens from Lebanese markets were examined for the presence of E. coli. Isolates were then screened for susceptibility using 19 antibiotics and two essential oils extracted from oregano plants.3. Of the 250 chickens tested, 80% were contaminated with E. coli. Main resistance was seen against amoxycillin, ampicillin, penicillin, tetracycline, tylosin, streptomycin and erythromycin. The highest rate of sensitivity was found in 86.1% of strains to Amoxycillin/Clavulanic acid, 80.09% to Tilmicosin. Both essential oils from Origanum syriacum (98%) and O. ehrenbergii (97.3%) showed promising potential in inhibiting the growth of the tested bacteria. Oil from O. syriacum exhibited superior efficacy against 200 E. coli strains, inhibiting 46.1% at 200 mg/l and all at 400 mg/l, while O. ehrenbergii oil showed slightly lower inhibition, affecting 41.6% at 200 mg/l and all at 400 mg/l.

1.家禽生产中抗菌剂的广泛使用可能会导致耐药菌的出现。本研究旨在确定从生鲜鸡肉中分离出的不同大肠杆菌菌株的流行率和耐药性,并调查使用黎巴嫩本地牛至精油作为替代品的可能性。 2. 总共检查了 250 只来自黎巴嫩市场的鸡,以确定是否存在大肠杆菌。然后使用 19 种抗生素和两种从牛至植物中提取的精油对分离物进行药敏性筛选。 在接受检测的 250 只鸡中,有 80% 受到大肠杆菌污染。大肠杆菌主要对氨苄西林、氨苄西林、青霉素、四环素、泰乐菌素、链霉素和红霉素产生抗药性。86.1%的菌株对阿莫西林/克拉维酸最敏感,80.09%的菌株对替米考星最敏感。牛至(Origanum syriacum)精油(98%)和牛至(O. ehrenbergii)精油(97.3%)在抑制受测细菌的生长方面都表现出了良好的潜力。O. syriacum精油对200株大肠杆菌的抑制效果较好,200毫克/升时抑制率为46.1%,400毫克/升时全部抑制;而O. ehrenbergii精油的抑制率稍低,200毫克/升时抑制率为41.6%,400毫克/升时全部抑制。
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引用次数: 0
Stimbiotic and wheat bran inclusion in maize or wheat-based diets for broiler chickens; effects on jejunal histomorphology, digesta oligosaccharides and caecal short-chain fatty-acids profiles. 在以玉米或小麦为基础的肉鸡日粮中添加刺激性益生菌和麦麸;对空肠组织形态学、消化道低聚糖和盲肠短链脂肪酸谱的影响。
IF 1.6 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2024-08-01 Epub Date: 2024-07-12 DOI: 10.1080/00071668.2024.2338535
S Veluri, G González-Ortiz, M R Bedford, O A Olukosi

1. A stimbiotic (STB) is any feed additive that stimulates caeca fibre fermentation, although the additive itself contributes little to the caeca short-chain fatty acid (SCFA) production. A 42 d experiment investigated the interactive effects of STB and wheat bran (WB) in broiler chickens receiving maize or wheat-based diets.2. The treatments were arranged in a 2 × 2 × 2 factorial (eight replicates each), the dietary factors being diet (maize-SBM or wheat-SBM), STB (with or without) and WB (0 or 50 g/kg). Jejunal tissue, gizzard, jejunal and ileal digesta and caecal contents were collected on d 18 and 42.3. Gizzard pH tended to decrease with STB (p = 0.06) supplementation and was lower in birds fed wheat- compared to maize-based diets on d 18 (p < 0.05). Birds receiving diets with WB had higher jejunum pH on d 18 (p < 0.05).4. Total short-chain fatty acids (SCFA) in the caeca on d 18 and isobutyrate on d 42 were higher (p < 0.05) for maize compared with wheat-based diets. However, on d 42, acetate, butyrate and total SCFA were higher (p < 0.05) for wheat-based compared with maize-based diets.5. On d 18, STB and WB inclusion increased villi height (VH; p < 0.05) and VH to crypt depth ratio (VH/CD), respectively (p < 0.05). On d 42, VH (p < 0.05) and VH/CD were higher in wheat-based diets (p < 0.05). The VH/CD ratio was lower with STB supplementation (p < 0.05). Marker-corrected pentose oligosaccharides (Pent)4 and (Pent)5 concentrations in the ileal digesta were reduced (p < 0.05) with STB supplementation. In addition, STB decreased (Pent)3 concentration in maize-, but not wheat-based diets (p < 0.05).6. In conclusion, both WB and STB influenced gastrointestinal pH and jejunum histomorphology of broilers without increasing oligosaccharide concentration in the ileum and SCFA in the caeca.

1.刺激性生物制剂(STB)是指任何能刺激盲肠纤维发酵的饲料添加剂,尽管添加剂本身对盲肠短链脂肪酸(SCFA)的产生几乎没有影响。一项为期 42 天的实验研究了 STB 和麦麸(WB)对以玉米或小麦为基础日粮的肉鸡的交互影响。 2.处理以 2 × 2 × 2 的因子排列(每组 8 个重复),日粮因子为日粮(玉米-SBM 或小麦-SBM)、STB(添加或不添加)和 WB(0 或 50 克/千克)。在第 18 天和第 42.3 天收集空肠组织、胗、空肠和回肠消化物以及盲肠内容物。添加 STB(p = 0.06)后,胗的 pH 值呈下降趋势,第 18 天,与饲喂玉米的鸟类相比,饲喂小麦的鸟类胗的 pH 值更低(p p p p p p p p p p p p p p p p 4 和回肠消化物中(Pent)5 的浓度在饲喂玉米的鸟类中降低(p 3),而在饲喂小麦的鸟类中没有降低(p 4)。
{"title":"Stimbiotic and wheat bran inclusion in maize or wheat-based diets for broiler chickens; effects on jejunal histomorphology, digesta oligosaccharides and caecal short-chain fatty-acids profiles.","authors":"S Veluri, G González-Ortiz, M R Bedford, O A Olukosi","doi":"10.1080/00071668.2024.2338535","DOIUrl":"10.1080/00071668.2024.2338535","url":null,"abstract":"<p><p>1. A stimbiotic (STB) is any feed additive that stimulates caeca fibre fermentation, although the additive itself contributes little to the caeca short-chain fatty acid (SCFA) production. A 42 d experiment investigated the interactive effects of STB and wheat bran (WB) in broiler chickens receiving maize or wheat-based diets.2. The treatments were arranged in a 2 × 2 × 2 factorial (eight replicates each), the dietary factors being diet (maize-SBM or wheat-SBM), STB (with or without) and WB (0 or 50 g/kg). Jejunal tissue, gizzard, jejunal and ileal digesta and caecal contents were collected on d 18 and 42.3. Gizzard pH tended to decrease with STB (<i>p</i> = 0.06) supplementation and was lower in birds fed wheat- compared to maize-based diets on d 18 (<i>p</i> < 0.05). Birds receiving diets with WB had higher jejunum pH on d 18 (<i>p</i> < 0.05).4. Total short-chain fatty acids (SCFA) in the caeca on d 18 and isobutyrate on d 42 were higher (<i>p</i> < 0.05) for maize compared with wheat-based diets. However, on d 42, acetate, butyrate and total SCFA were higher (<i>p</i> < 0.05) for wheat-based compared with maize-based diets.5. On d 18, STB and WB inclusion increased villi height (VH; <i>p</i> < 0.05) and VH to crypt depth ratio (VH/CD), respectively (<i>p</i> < 0.05). On d 42, VH (<i>p</i> < 0.05) and VH/CD were higher in wheat-based diets (<i>p</i> < 0.05). The VH/CD ratio was lower with STB supplementation (<i>p</i> < 0.05). Marker-corrected pentose oligosaccharides (Pent)<sub>4</sub> and (Pent)<sub>5</sub> concentrations in the ileal digesta were reduced (<i>p</i> < 0.05) with STB supplementation. In addition, STB decreased (Pent)<sub>3</sub> concentration in maize-, but not wheat-based diets (<i>p</i> < 0.05).6. In conclusion, both WB and STB influenced gastrointestinal pH and jejunum histomorphology of broilers without increasing oligosaccharide concentration in the ileum and SCFA in the caeca.</p>","PeriodicalId":9322,"journal":{"name":"British Poultry Science","volume":" ","pages":"465-477"},"PeriodicalIF":1.6,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141589711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
New primers for sex identification in the Chukar partridges (Alectoris chukar). 用于鉴定楚卡鹧鸪(Alectoris chukar)性别的新引物。
IF 1.6 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2024-08-01 Epub Date: 2024-05-08 DOI: 10.1080/00071668.2024.2332723
X X Jia, W Q Chen, X J Tang, J X Lu, Y F Fan, Y S Gao

1. Male and female Chukar partridges are difficult to differentiate based on their morphology or by the Chromobox-Helicase-DNA binding (CHD) during early growth.2. The current study developed a novel, simple, low-cost and rapid sexing protocol for Chukar partridges based on the newly defined sexing gene ubiquitin-associated protein 2 (UBAP2).3. The length of polymorphism between UBAP2-W and UBAP2-Z homologous genes allows for easy sex discrimination in this species. Molecular sexing analysis was based on the simultaneous amplification of both genes, resulting in two distinct amplicons (947 bp and 535 bp) in heterogametic females and only a single band (535 bp) in homogametic males, which is easy to detect with agarose gel electrophoresis.4. This technique is simple and convenient for genetic sex determination in Chukar partridges.

1.2. 本研究基于新定义的性别鉴定基因泛素相关蛋白 2(UBAP2),开发了一种新颖、简单、低成本且快速的楚科鹧鸪性别鉴定方案。 3. UBAP2-W和UBAP2-Z同源基因之间的多态性长度使该物种的性别鉴定变得容易。分子性别分析基于两个基因的同时扩增,在异配雌性中产生两个不同的扩增子(947 bp 和 535 bp),而在同配雄性中只有一个单一的条带(535 bp),这在琼脂糖凝胶电泳中很容易检测到。 4. 该技术简单方便,可用于楚科鹧鸪的遗传性别鉴定。
{"title":"New primers for sex identification in the Chukar partridges (<i>Alectoris chukar</i>).","authors":"X X Jia, W Q Chen, X J Tang, J X Lu, Y F Fan, Y S Gao","doi":"10.1080/00071668.2024.2332723","DOIUrl":"10.1080/00071668.2024.2332723","url":null,"abstract":"<p><p>1. Male and female Chukar partridges are difficult to differentiate based on their morphology or by the Chromobox-Helicase-DNA binding (<i>CHD</i>) during early growth.2. The current study developed a novel, simple, low-cost and rapid sexing protocol for Chukar partridges based on the newly defined sexing gene ubiquitin-associated protein 2 (<i>UBAP2</i>).3. The length of polymorphism between <i>UBAP2</i>-W and <i>UBAP2</i>-Z homologous genes allows for easy sex discrimination in this species. Molecular sexing analysis was based on the simultaneous amplification of both genes, resulting in two distinct amplicons (947 bp and 535 bp) in heterogametic females and only a single band (535 bp) in homogametic males, which is easy to detect with agarose gel electrophoresis.4. This technique is simple and convenient for genetic sex determination in Chukar partridges.</p>","PeriodicalId":9322,"journal":{"name":"British Poultry Science","volume":" ","pages":"424-428"},"PeriodicalIF":1.6,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140875921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genetic analysis of a Kaijiang duck conservation population through genome-wide scan. 通过全基因组扫描对开江鸭保护种群进行遗传分析。
IF 1.6 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2024-08-01 Epub Date: 2024-05-13 DOI: 10.1080/00071668.2024.2335937
Z Shen, T Zhang, G Twumasi, J Zhang, J Wang, Y Xi, R Wang, J Wang, R Zhang, H Liu

1. The Kaijiang duck is a native Chinese breed known for its excellent egg laying performance, killing-out percentage (88.57%), and disease resistance. The assessment of population genetic structure is the basis for understanding the genetics of indigenous breeds and for their protection and management.2. In this study, whole-genome sequencing was performed on 60 Kaijiang ducks to identify genetic variations and investigate the population structure. Homozygosity (ROH) analysis was conducted to assess inbreeding levels in the population.3. The study revealed a moderate level of inbreeding, indicated by an average inbreeding coefficient of 0.1043. This may impact the overall genetic diversity.4. Genomic Regions of Interest identified included 168 genomic regions exhibiting high levels of autozygosity. These regions were associated with processes including muscle growth, pigmentation, neuromodulation, and growth and reproduction.5. The significance of these pathways indicated their potential role in shaping the desirable traits of the Kaijiang duck. These findings provide insights into the genetic basis of the Kaijiang duck's desirable traits and can inform future breeding and conservation efforts.

1.开江鸭是中国土生土长的鸭种,以产蛋率高、淘汰率高(88.57%)、抗病力强而著称。评估种群遗传结构是了解本土品种遗传学及其保护和管理的基础。 2. 本研究对 60 只开江鸭进行了全基因组测序,以确定遗传变异并调查种群结构。3. 研究显示,近亲繁殖的程度适中,平均近亲繁殖系数为 0.1043。4. 确定的基因组感兴趣区包括 168 个表现出高水平同源性的基因组区域。这些区域与肌肉生长、色素沉着、神经调节以及生长和繁殖等过程有关。 5 这些途径的重要性表明它们在塑造开江鸭理想性状方面的潜在作用。这些发现有助于深入了解开江鸭理想性状的遗传基础,并为未来的育种和保护工作提供参考。
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引用次数: 0
Characterization of circRNA expression profiles associated with non-Mendelian inheritance in feather growth of chickens. 与鸡羽毛生长的非孟德尔遗传相关的 circRNA 表达谱特征。
IF 1.6 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2024-08-01 Epub Date: 2024-05-08 DOI: 10.1080/00071668.2024.2339485
M Qiu, C Yu, S Zhu, C Hu, L Yang, X Song, B Xia, X Jiang, H Du, Q Li, Z Zhang, C Yang

1. Non-coding RNAs, such as miRNAs, play a crucial role in chicken feather growth rate. However, circular RNA (circRNA) expression profiles in fast- and slow-feathering chickens that follow and do not follow Mendelian inheritance are unclear.2. The circRNA expression profiles was analysed by RNA sequencing of hair follicles of slow-feathering chickens that follow genetic rules and fast-feathering chickens that did not follow genetic rules. Differentially expressed circRNA-miRNA-mRNA competing endogenous RNA (ceRNA) network was then constructed and the key factors and regulation mechanisms controlling feather growth rate were identified.3. The results revealed that 67 circRNAs were significantly differentially expressed in hens, including 22 up-regulated and 45 down-regulated circRNAs in non-Mendelian inheritance-mediated fast-feathering hens compared with Mendelian inheritance-mediated slow-feathering hens. In addition, 16 significantly differentially expressed circRNAs were identified in cockerels, including nine up-regulated and seven down-regulated circRNAs in non-Mendelian inheritance-mediated fast- compared with Mendelian inheritance-mediated slow-feathering cocks. Moreover, circRNA-mediated ceRNA regulation of hair follicle formation was particularly abundant in the Jak-STAT, Wnt and Toll-like receptor signalling pathways. Furthermore, circABI3BP was seen to be a crucial circRNA in regulating feather growth rate, by binding with gga-miR-1649-5p to regulate SSTR2 expression.4. In conclusion, this study analysed circRNA expression profiles in fast- and slow-feathering chickens that follow and do not follow Mendelian inheritance, which laid the foundation for understanding the role of circRNA in chicken feather growth rate.

1.非编码 RNA(如 miRNA)在鸡的羽毛生长速度中起着至关重要的作用。然而,遵循和不遵循孟德尔遗传的快羽鸡和慢羽鸡的环状 RNA(circRNA)表达谱尚不清楚。 2. 通过对遵循遗传规则的慢羽鸡和不遵循遗传规则的快羽鸡的毛囊进行 RNA 测序,分析了环状 RNA 的表达谱。结果发现,67个circRNA在母鸡体内显著差异表达,其中非孟德尔遗传介导的快羽鸡与孟德尔遗传介导的慢羽鸡相比,上调22个circRNA,下调45个circRNA。此外,还在公鸡体内发现了 16 个表达明显不同的 circRNA,其中包括非孟德尔遗传介导的快羽公鸡与孟德尔遗传介导的慢羽公鸡相比,上调了 9 个 circRNA,下调了 7 个 circRNA。此外,在 Jak-STAT、Wnt 和 Toll 样受体信号通路中,circRNA 介导的 ceRNA 对毛囊形成的调控尤其丰富。此外,circABI3BP通过与gga-miR-1649-5p结合调控SSTR2的表达,被认为是调控羽毛生长率的关键circRNA。 总之,本研究分析了遵循和不遵循孟德尔遗传的快羽鸡和慢羽鸡的circRNA表达谱,为了解circRNA在鸡羽毛生长率中的作用奠定了基础。
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引用次数: 0
Quercetin ameliorates lipid deposition in primary hepatocytes of the chicken embryo. 槲皮素可改善鸡胚胎原代肝细胞中的脂质沉积。
IF 1.6 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2024-08-01 Epub Date: 2024-05-10 DOI: 10.1080/00071668.2024.2332717
Y Feng, C Zhao, T Li, M Wang, B R Serrano, A R Barcenas, L Qu, W Zhao, M Shen

1. The accumulation of excessive fat plays a role in the development of non-alcoholic fatty liver disease (NAFLD) and phytogenic feed additives have the potential to ameliorate this. This study involved the isolation and culture of primary hepatocytes from chicken embryos to establish a model of hepatic steatosis induced by oleic acid/dexamethasone (OA/DEX). Lipid accumulation and cell viability were assessed using Nile Red staining, Oil Red O staining and cell count Kit -8 (CCK8) following treatment with varying concentrations of quercetin (Que). The potential mechanism by which Que exerts its effects was preliminarily investigated.2. The results indicated that OA effectively treated lipid accumulation in hepatocytes. There was no notable variance in cell proliferation between the normal and OA/DEX groups when subjected to Que treatment at concentrations of 1000 ng/ml and 10 000 ng/ml. Triglycerides and cholesterol (low and high density) decreased with Que treatment, with the most substantial reduction observed at 10 000 ng/ml.3. Gene expression levels decreased to levels similar to those in the control groups. Western blot data demonstrated that sterol regulatory element-binding protein 1 (SREBP-1) protein expression correlated with its mRNA expression level. Que mitigated lipid accumulation through the alpha serine/threonine protein kinase (AKT) and extracellular signal-regulated kinase (ERK) pathways. Expression levels of lipid-related genes (APOB, PPARα, CYP3A5 and SREBP-1) decreased to levels similar to the control groups. Western blot data demonstrated that the SREBP-1 protein expression correlated with its mRNA expression level.4. Supplementation with Que ameliorated lipid accumulation through AKT and ERK signalling pathway in OA/DEX-induced high-fat hepatocytes.

1.过多脂肪的积累在非酒精性脂肪肝(NAFLD)的发病过程中起着一定的作用,而植物性饲料添加剂有可能改善这种情况。本研究涉及从鸡胚胎中分离和培养原代肝细胞,以建立油酸/地塞米松(OA/DEX)诱导的肝脂肪变性模型。在使用不同浓度的槲皮素(Que)处理后,使用尼罗红染色法、油红 O 染色法和细胞计数试剂盒 -8 (CCK8) 评估脂质积累和细胞活力。结果表明,OA 能有效处理肝细胞中的脂质积累。当阙浓度为 1000 ng/ml 和 10 000 ng/ml 时,正常组和 OA/DEX 组的细胞增殖没有明显差异。甘油三酯和胆固醇(低密度和高密度)随着阙处理的进行而下降,在 10 000 ng/ml 浓度时下降幅度最大。 基因表达水平下降到与对照组相似的水平。Western 印迹数据显示,固醇调节元件结合蛋白 1(SREBP-1)蛋白的表达与其 mRNA 的表达水平相关。阙通过α丝氨酸/苏氨酸蛋白激酶(AKT)和细胞外信号调节激酶(ERK)途径缓解脂质积累。脂质相关基因(APOB、PPARα、CYP3A5 和 SREBP-1)的表达水平降至与对照组相似的水平。Western 印迹数据表明,SREBP-1 蛋白表达与其 mRNA 表达水平相关。 补充阙通过 AKT 和 ERK 信号通路改善 OA/DEX 诱导的高脂肝细胞的脂质积累。
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引用次数: 0
Comparative transcriptomic analyses reveal key genes underlying melanin distribution during embryonic development in geese (Anser anser). 比较转录组分析揭示了鹅(Anser anser)胚胎发育过程中黑色素分布的关键基因。
IF 1.6 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2024-08-01 Epub Date: 2024-05-15 DOI: 10.1080/00071668.2024.2335943
Y Yang, C Wang, Y Liu, G Li, X Wang, H Wang, D He

1. Melanin distribution typically exhibits a gradient dilution along the dorsal-ventral axis of the body, including in domestic geese. However, the specific genes and molecular mechanisms responsible for this melanin distribution pattern remain incompletely understood.2. The transcriptomic comparisons were conducted at three embryonic stages, specifically on embryonic d 15 (E15), 22 (E22), and 29 (E29), between the pigmented dorsal skin and the depigmented distal foot.3. Differentially expressed genes (DEGs) associated with melanin synthesis were identified, particularly TYR, TYRP1, and EDNRB2, which exhibited significantly higher expression levels in the dorsal skin at E15 and E22. However, expression levels significantly decreased in later stages (E29).4. The ASIP gene showed remarkably high-expression levels in the distal feet compared to the dorsal skin post-E22 stage (log2FC: 5.31/6.88 at E22/E29). Gene Ontology (GO) enrichment analysis detected eight terms associated with melanin synthesis and melanosome formation (p < 0.05), including melanosome membrane (GO: 0033162) and melanin biosynthetic process (GO: 0042438). Additionally, KEGG pathway analysis showed significant enrichment of the melanogenesis pathway (hsa004916) at d 22 (E22).

1.黑色素的分布通常沿着身体的背腹轴呈梯度稀释,家鹅也是如此。2. 转录组比较在三个胚胎阶段进行,特别是胚胎第 15 (E15)、22 (E22) 和 29 (E29)天,在色素沉着的背侧皮肤和色素脱失的远足之间进行。发现了与黑色素合成相关的差异表达基因(DEGs),尤其是 TYR、TYRP1 和 EDNRB2,它们在 E15 和 E22 期的背侧皮肤中的表达水平显著较高。4. ASIP 基因在 E22 阶段后在远足皮肤的表达水平明显高于在背侧皮肤的表达水平(E22/E29 阶段的 log2FC:5.31/6.88)。基因本体(GO)富集分析检测到八个与黑色素合成和黑色素小体形成相关的术语(p
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引用次数: 0
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British Poultry Science
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