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Characterization of circRNA expression profiles associated with non-Mendelian inheritance in feather growth of chickens. 与鸡羽毛生长的非孟德尔遗传相关的 circRNA 表达谱特征。
IF 1.6 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2024-08-01 Epub Date: 2024-05-08 DOI: 10.1080/00071668.2024.2339485
M Qiu, C Yu, S Zhu, C Hu, L Yang, X Song, B Xia, X Jiang, H Du, Q Li, Z Zhang, C Yang

1. Non-coding RNAs, such as miRNAs, play a crucial role in chicken feather growth rate. However, circular RNA (circRNA) expression profiles in fast- and slow-feathering chickens that follow and do not follow Mendelian inheritance are unclear.2. The circRNA expression profiles was analysed by RNA sequencing of hair follicles of slow-feathering chickens that follow genetic rules and fast-feathering chickens that did not follow genetic rules. Differentially expressed circRNA-miRNA-mRNA competing endogenous RNA (ceRNA) network was then constructed and the key factors and regulation mechanisms controlling feather growth rate were identified.3. The results revealed that 67 circRNAs were significantly differentially expressed in hens, including 22 up-regulated and 45 down-regulated circRNAs in non-Mendelian inheritance-mediated fast-feathering hens compared with Mendelian inheritance-mediated slow-feathering hens. In addition, 16 significantly differentially expressed circRNAs were identified in cockerels, including nine up-regulated and seven down-regulated circRNAs in non-Mendelian inheritance-mediated fast- compared with Mendelian inheritance-mediated slow-feathering cocks. Moreover, circRNA-mediated ceRNA regulation of hair follicle formation was particularly abundant in the Jak-STAT, Wnt and Toll-like receptor signalling pathways. Furthermore, circABI3BP was seen to be a crucial circRNA in regulating feather growth rate, by binding with gga-miR-1649-5p to regulate SSTR2 expression.4. In conclusion, this study analysed circRNA expression profiles in fast- and slow-feathering chickens that follow and do not follow Mendelian inheritance, which laid the foundation for understanding the role of circRNA in chicken feather growth rate.

1.非编码 RNA(如 miRNA)在鸡的羽毛生长速度中起着至关重要的作用。然而,遵循和不遵循孟德尔遗传的快羽鸡和慢羽鸡的环状 RNA(circRNA)表达谱尚不清楚。 2. 通过对遵循遗传规则的慢羽鸡和不遵循遗传规则的快羽鸡的毛囊进行 RNA 测序,分析了环状 RNA 的表达谱。结果发现,67个circRNA在母鸡体内显著差异表达,其中非孟德尔遗传介导的快羽鸡与孟德尔遗传介导的慢羽鸡相比,上调22个circRNA,下调45个circRNA。此外,还在公鸡体内发现了 16 个表达明显不同的 circRNA,其中包括非孟德尔遗传介导的快羽公鸡与孟德尔遗传介导的慢羽公鸡相比,上调了 9 个 circRNA,下调了 7 个 circRNA。此外,在 Jak-STAT、Wnt 和 Toll 样受体信号通路中,circRNA 介导的 ceRNA 对毛囊形成的调控尤其丰富。此外,circABI3BP通过与gga-miR-1649-5p结合调控SSTR2的表达,被认为是调控羽毛生长率的关键circRNA。 总之,本研究分析了遵循和不遵循孟德尔遗传的快羽鸡和慢羽鸡的circRNA表达谱,为了解circRNA在鸡羽毛生长率中的作用奠定了基础。
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引用次数: 0
Quercetin ameliorates lipid deposition in primary hepatocytes of the chicken embryo. 槲皮素可改善鸡胚胎原代肝细胞中的脂质沉积。
IF 1.6 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2024-08-01 Epub Date: 2024-05-10 DOI: 10.1080/00071668.2024.2332717
Y Feng, C Zhao, T Li, M Wang, B R Serrano, A R Barcenas, L Qu, W Zhao, M Shen

1. The accumulation of excessive fat plays a role in the development of non-alcoholic fatty liver disease (NAFLD) and phytogenic feed additives have the potential to ameliorate this. This study involved the isolation and culture of primary hepatocytes from chicken embryos to establish a model of hepatic steatosis induced by oleic acid/dexamethasone (OA/DEX). Lipid accumulation and cell viability were assessed using Nile Red staining, Oil Red O staining and cell count Kit -8 (CCK8) following treatment with varying concentrations of quercetin (Que). The potential mechanism by which Que exerts its effects was preliminarily investigated.2. The results indicated that OA effectively treated lipid accumulation in hepatocytes. There was no notable variance in cell proliferation between the normal and OA/DEX groups when subjected to Que treatment at concentrations of 1000 ng/ml and 10 000 ng/ml. Triglycerides and cholesterol (low and high density) decreased with Que treatment, with the most substantial reduction observed at 10 000 ng/ml.3. Gene expression levels decreased to levels similar to those in the control groups. Western blot data demonstrated that sterol regulatory element-binding protein 1 (SREBP-1) protein expression correlated with its mRNA expression level. Que mitigated lipid accumulation through the alpha serine/threonine protein kinase (AKT) and extracellular signal-regulated kinase (ERK) pathways. Expression levels of lipid-related genes (APOB, PPARα, CYP3A5 and SREBP-1) decreased to levels similar to the control groups. Western blot data demonstrated that the SREBP-1 protein expression correlated with its mRNA expression level.4. Supplementation with Que ameliorated lipid accumulation through AKT and ERK signalling pathway in OA/DEX-induced high-fat hepatocytes.

1.过多脂肪的积累在非酒精性脂肪肝(NAFLD)的发病过程中起着一定的作用,而植物性饲料添加剂有可能改善这种情况。本研究涉及从鸡胚胎中分离和培养原代肝细胞,以建立油酸/地塞米松(OA/DEX)诱导的肝脂肪变性模型。在使用不同浓度的槲皮素(Que)处理后,使用尼罗红染色法、油红 O 染色法和细胞计数试剂盒 -8 (CCK8) 评估脂质积累和细胞活力。结果表明,OA 能有效处理肝细胞中的脂质积累。当阙浓度为 1000 ng/ml 和 10 000 ng/ml 时,正常组和 OA/DEX 组的细胞增殖没有明显差异。甘油三酯和胆固醇(低密度和高密度)随着阙处理的进行而下降,在 10 000 ng/ml 浓度时下降幅度最大。 基因表达水平下降到与对照组相似的水平。Western 印迹数据显示,固醇调节元件结合蛋白 1(SREBP-1)蛋白的表达与其 mRNA 的表达水平相关。阙通过α丝氨酸/苏氨酸蛋白激酶(AKT)和细胞外信号调节激酶(ERK)途径缓解脂质积累。脂质相关基因(APOB、PPARα、CYP3A5 和 SREBP-1)的表达水平降至与对照组相似的水平。Western 印迹数据表明,SREBP-1 蛋白表达与其 mRNA 表达水平相关。 补充阙通过 AKT 和 ERK 信号通路改善 OA/DEX 诱导的高脂肝细胞的脂质积累。
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引用次数: 0
Comparative transcriptomic analyses reveal key genes underlying melanin distribution during embryonic development in geese (Anser anser). 比较转录组分析揭示了鹅(Anser anser)胚胎发育过程中黑色素分布的关键基因。
IF 1.6 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2024-08-01 Epub Date: 2024-05-15 DOI: 10.1080/00071668.2024.2335943
Y Yang, C Wang, Y Liu, G Li, X Wang, H Wang, D He

1. Melanin distribution typically exhibits a gradient dilution along the dorsal-ventral axis of the body, including in domestic geese. However, the specific genes and molecular mechanisms responsible for this melanin distribution pattern remain incompletely understood.2. The transcriptomic comparisons were conducted at three embryonic stages, specifically on embryonic d 15 (E15), 22 (E22), and 29 (E29), between the pigmented dorsal skin and the depigmented distal foot.3. Differentially expressed genes (DEGs) associated with melanin synthesis were identified, particularly TYR, TYRP1, and EDNRB2, which exhibited significantly higher expression levels in the dorsal skin at E15 and E22. However, expression levels significantly decreased in later stages (E29).4. The ASIP gene showed remarkably high-expression levels in the distal feet compared to the dorsal skin post-E22 stage (log2FC: 5.31/6.88 at E22/E29). Gene Ontology (GO) enrichment analysis detected eight terms associated with melanin synthesis and melanosome formation (p < 0.05), including melanosome membrane (GO: 0033162) and melanin biosynthetic process (GO: 0042438). Additionally, KEGG pathway analysis showed significant enrichment of the melanogenesis pathway (hsa004916) at d 22 (E22).

1.黑色素的分布通常沿着身体的背腹轴呈梯度稀释,家鹅也是如此。2. 转录组比较在三个胚胎阶段进行,特别是胚胎第 15 (E15)、22 (E22) 和 29 (E29)天,在色素沉着的背侧皮肤和色素脱失的远足之间进行。发现了与黑色素合成相关的差异表达基因(DEGs),尤其是 TYR、TYRP1 和 EDNRB2,它们在 E15 和 E22 期的背侧皮肤中的表达水平显著较高。4. ASIP 基因在 E22 阶段后在远足皮肤的表达水平明显高于在背侧皮肤的表达水平(E22/E29 阶段的 log2FC:5.31/6.88)。基因本体(GO)富集分析检测到八个与黑色素合成和黑色素小体形成相关的术语(p
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引用次数: 0
Expression of genes related to ileal barrier function in heritage and modern broiler chickens. 传统和现代肉鸡回肠屏障功能相关基因的表达。
IF 1.6 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2024-08-01 Epub Date: 2024-05-08 DOI: 10.1080/00071668.2024.2337187
K R Kpodo, K B Miska, L L Schreier, M Proszkowiec-Weglarz

1. An experiment was conducted to determine differences in the expression of genes encoding intestinal barrier proteins between fast, medium and slow-growing chickens. Chicken breeds Athens Canadian Random Bred (ACRB), Longenecker's Heritage (LHR), RedBro, Hubbard H1 (HH1), Cobb500 and Ross708 were raised from hatch for 35 d.2. Ileal samples were collected at embryonic day E19 (-2 days post-hatch), hatch and d 7, 14, 21, 28 and 35 post-hatch to assess the expression of genes encoding tight junction proteins (claudins, CLDN; occludin, OCLN; zonula occludens, ZO; and junctional adhesion molecules, JAM), mucin (Muc2), immunoglobulin A (IgA), polymeric immunoglobulin receptor (pIgR) and fatty acid binding protein (FABP2).3. Expression of CLDN-1 was increased (p < 0.0001) in LHR compared to Cobb500 while CLDN-5 was increased (p < 0.0001) in ACRB, HH1, RedBro and Ross708 compared to LHR as well as in ACRB compared to Cob500. Occludin was upregulated (p = 0.01) in ACRB and LHR compared to Ross708 at d 14 post-hatch. Expression of ZO-1 was upregulated (p = 0.001) in LHR compared to Ross708, HH1 and Cobb500. Tight junction genes, except CLDN-4, JAM-2 and JAM-3 were downregulated (p < 0.0001) at hatch and d 7 post-hatch. Expression of Muc2 was increased (p < 0.0001) in LHR compared to RedBro and from -2 d to d 7 post-hatch.4. Immunoglobulin A was increased (p = 0.001) in LHR compared to Ross708 and HH1 at -2 d post-hatch and in LHR compared to ACRB, Cobb500 and Ross708 at hatch. In addition, IgA expression was increased in all breeds at d 14 post-hatch while pIgR was upregulated (p = 0.02) in Cobb500 and Ross708 compared to ACRB, HH1, LHR and RedBro at hatch.5. The gene expression patterns suggest that selection for growth may have not induced changes in junctional complexes and immune defence genes. However, the results confirmed that the expression of these genes is age dependent.

1.我们进行了一项实验,以确定快速生长鸡、中速生长鸡和慢速生长鸡肠道屏障蛋白编码基因表达的差异。鸡种雅典加拿大随机培育鸡(ACRB)、Longenecker's Heritage(LHR)、RedBro、Hubbard H1(HH1)、Cobb500 和 Ross708 从孵化开始饲养 35 d。.在胚胎 E19 天(孵化后 2 天)、孵化后第 7、14、21、28 和 35 天收集回肠样本,以评估编码紧密连接蛋白(claudins、CLDN;闭锁蛋白,OCLN;闭锁带,ZO;交界粘附分子,JAM)、粘蛋白(Muc2)、免疫球蛋白 A(IgA)、聚合免疫球蛋白受体(pIgR)和脂肪酸结合蛋白(FABP2)的基因表达。与 Ross708 相比,ACRB 和 LHR 在孵化后第 14 天的 CLDN-1 表达量增加(p p = 0.01)。与 Ross708、HH1 和 Cobb500 相比,LHR 中 ZO-1 的表达上调(p = 0.001)。除 CLDN-4、JAM-2 和 JAM-3 外,其他紧密连接基因在孵化时和孵化后第 7 天均出现下调(p < 0.0001)。与孵化后第 2 d 的 Ross708 和 HH1 相比,LHR 的 Muc2 表达量增加(p p = 0.001),与孵化时的 ACRB、Cobb500 和 Ross708 相比,LHR 的 Muc2 表达量增加(p p = 0.001)。此外,在孵化后第 14 d,所有品种的 IgA 表达量都有所增加,而与孵化时的 ACRB、HH1、LHR 和 RedBro 相比,Cobb500 和 Ross708 的 pIgR 表达量上调(p = 0.02)。 基因表达模式表明,生长选择可能并未诱导连接复合体和免疫防御基因发生变化。不过,研究结果证实,这些基因的表达与年龄有关。
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引用次数: 0
Phenotypic and molecular characterisation of Salmonella spp. isolates in healthy poultry. 健康家禽中沙门氏菌属分离物的表型和分子特征。
IF 1.6 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2024-08-01 Epub Date: 2024-05-08 DOI: 10.1080/00071668.2024.2337180
V Lucca, K A Borges, T Q Furian, G Z Chitolina, A F Streck, D T da Rocha, H L de Souza Moraes, V P Nascimento

1. Epidemiological surveillance of Salmonella spp. serves as a primary tool for maintaining the health of poultry flocks. Characterising circulating serotypes is crucial for implementing control and prevention measures. This study conducted phenotypic and molecular characterisation of S. enterica Pullorum, S. enterica Heidelberg, and S. enterica Corvalis isolated from broiler chickens during slaughtering.2. All strains were susceptible to gentamicin, neomycin and norfloxacin. However, resistance rates exceeded 50% for ciprofloxacin and tiamulin, irrespective of the serotype. Approximately 64% of strains were classified as multidrug-resistant, with S. enterica Heidelberg strains exhibiting significantly higher overall resistance. The isolates demonstrated the ability to adhere and produce biofilm at a minimum of three temperatures, with S. enterica Pullorum capable of biofilm production at all temperatures encountered during poultry rearing.3. Each strain possessed between two and seven different virulence-associated genes. Genetic similarity, as indicated by pulsed field gel electrophoresis, exceeded 90% for all three serotypes and strains were classified in the R5 ribotype by PCR, regardless of serotype. Sequencing revealed high similarity among all strains, with homology ranging from 99.61 to 100% and all were classified to a single cluster.4. The results suggested a clonal relationship among the strains, indicating the possible circulation of a unique clonal group of S. enterica Pullorum in the southern region of Brazil.

1.沙门氏菌属流行病学监测是维护禽群健康的主要工具。确定循环血清型对于实施防控措施至关重要。本研究对屠宰过程中从肉鸡中分离出的普劳伦肠炎沙门氏菌、海德堡肠炎沙门氏菌和科瓦利斯肠炎沙门氏菌进行了表型和分子鉴定。 所有菌株均对庆大霉素、新霉素和诺氟沙星敏感。然而,无论血清型如何,对环丙沙星和替米考星的耐药率均超过 50%。约 64% 的菌株被归类为多重耐药菌株,其中海德堡肠杆菌菌株的总体耐药率明显更高。分离菌株在至少三种温度下都能粘附并形成生物膜,其中 S. enterica Pullorum 在家禽饲养过程中遇到的所有温度下都能形成生物膜。 每个菌株都有 2 到 7 个不同的毒力相关基因。脉冲场凝胶电泳显示,所有三个血清型的基因相似度都超过了 90%,而且无论血清型如何,菌株都通过 PCR 被归类为 R5 核型。测序结果表明,所有菌株之间具有高度相似性,同源性从 99.61%到 100%不等,而且所有菌株都被归入一个群组。 结果表明,这些菌株之间存在克隆关系,表明在巴西南部地区可能存在一个独特的普氏肠杆菌克隆群。
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引用次数: 0
Transcriptome analysis of long non-coding RNA associated with embryonic muscle development in chickens. 与鸡胚胎肌肉发育相关的长非编码 RNA 转录组分析
IF 1.6 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2024-08-01 Epub Date: 2024-05-13 DOI: 10.1080/00071668.2024.2335935
Y Yuan, W Duan, N Yang, C Sun, Q Nie, J Li, L Lian

1. Skeletal muscle is an important component of chicken carcass. In chickens, the number of muscle fibres is fixed during the embryonic period, and muscle development during the embryonic period determines the muscle development potential after hatching.2. Beijing-You (BY) and Cornish (CN) chickens show completely different growth rates and body types, and two breeds were used in this study to explore the role of lncRNAs in muscle development during different chicken embryonic periods. A systematic analysis of lncRNAs and mRNAs were conducted in the pectoral muscle tissues of BY and CN chickens at embryonic days 11 (ED11), 13 (ED13), 15 (ED15), 17 (ED17), and 1-day-old (D1) using RNA-seq. A total of 4,104 differentially expressed transcripts (DETs) were identified among the five stages, including 2,359 lncRNAs and 1,745 mRNAs.3. The number of DETs between the two breeds at ED17 (1,658 lncRNAs and 1,016 mRNAs) was much higher than the total number of DET at all the other stages (692 lncRNAs and 729 mRNAs), indicating that the two breeds show the largest difference in gene regulation at ED17.4. Correlation analysis was performed for all differentially expressed lncRNAs and mRNAs during the five periods. Forty-three, cis interaction pairs of lncRNA-mRNA related to chicken muscle development were predicted. The expression of four pairs was verified, and the results showed MSTRG.12395.2-FGFBP2 and MSTRG.18590.6-FMOD were significantly up-regulated in CN at ED11 compared to BY and might be important candidate genes for embryonic muscle development.

1.骨骼肌是鸡胴体的重要组成部分。鸡胚胎期的肌纤维数量是固定的,胚胎期的肌肉发育决定了孵化后的肌肉发育潜力。 2. 本研究以北京优胜鸡(BY)和康尼什鸡(CN)为研究对象,探讨lncRNAs在不同鸡胚胎期肌肉发育中的作用。利用RNA-seq技术对BY鸡和CN鸡在胚胎第11天(ED11)、13天(ED13)、15天(ED15)、17天(ED17)和1日龄(D1)时的胸肌组织中的lncRNAs和mRNAs进行了系统分析。五个阶段共鉴定出 4,104 个差异表达转录本 (DETs),包括 2,359 个 lncRNAs 和 1,745 个 mRNAs。两个品种在 ED17 阶段的 DET 数量(1 658 个 lncRNA 和 1 016 个 mRNA)远高于所有其他阶段的 DET 总数(692 个 lncRNA 和 729 个 mRNA),表明两个品种在 ED17 阶段的基因调控差异最大。对五个阶段中所有差异表达的 lncRNA 和 mRNA 进行了相关性分析。预测了43对与鸡肌肉发育相关的lncRNA-mRNA顺式相互作用。结果表明,与BY相比,MSTRG.12395.2-FGFBP2和MSTRG.18590.6-FMOD在ED11期的CN中显著上调,可能是胚胎肌肉发育的重要候选基因。
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引用次数: 0
Dietary fat lowers ileal endogenous amino acid losses in broiler chickens. 日粮脂肪可降低肉鸡回肠内源性氨基酸的损失。
IF 1.6 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2024-08-01 Epub Date: 2024-06-03 DOI: 10.1080/00071668.2024.2346317
T H Whitehouse, F Zaefarian, M R Abdollahi, V Ravindran

1. An experiment was conducted to determine the effect of the source of fat (soybean oil or tallow) on the ileal endogenous amino acid (EAA) losses in broilers.2. Three nitrogen (N)-free diets; a control diet with no added fat and test diets with 60 g/kg of either soybean oil or tallow were formulated. Titanium dioxide (5 g/kg) was added to all diets as an indigestible marker. Each diet was assigned to six replicate cages (eight birds per cage) from d 18 to 21 post-hatch. On d 21, the digesta were collected from the lower half of the ileum.3. The endogenous losses of nitrogen and amino acids (AA) were lower (p = 0.08; p = 0.001) in broilers fed diets with soybean oil or tallow, respectively, compared to those fed the diet with no fat. Source of fat had no influence (p > 0.05) on EAA losses.4. The most abundant AA in the ileal endogenous protein was glutamic acid, followed by aspartic acid, threonine, leucine, serine, valine and proline. In general, the concentrations of AA in the endogenous protein were lower (p < 0.05) with added fat. The exceptions were methionine, cysteine, proline and serine, which were unaffected. The effect of fat source on the AA contents of endogenous protein were inconsistent and differed depending on the AA.5. The inclusion of fats decreased EAA losses which implied they have beneficial effects beyond direct energy contribution. It can be proposed that the reduction of EAA flow may be an additional mechanism contributing to the extra-caloric effect of dietary fats.

1.2. 配制了三种无氮(N)日粮;不添加脂肪的对照日粮和添加 60 克/千克大豆油或牛脂的试验日粮。所有日粮中都添加了二氧化钛(5 克/千克)作为难消化标记物。从孵化后第 18 天到第 21 天,将每种日粮分配到六个重复笼中(每个笼子八只鸟)。与饲喂不含脂肪的日粮相比,饲喂大豆油或牛油日粮的肉鸡氮和氨基酸(AA)的内源损失分别较低(p = 0.08;p = 0.001)。脂肪来源对 EAA 的损失没有影响(p > 0.05)。 回肠内源蛋白中含量最高的 AA 是谷氨酸,其次是天冬氨酸、苏氨酸、亮氨酸、丝氨酸、缬氨酸和脯氨酸。一般来说,内源蛋白中的 AA 浓度较低(p
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引用次数: 0
Dietary ratio of linoleic to alpha-linolenic acid affects the reproductive performance of Japanese quail. 亚油酸与α-亚麻酸的膳食比例对日本鹌鹑繁殖性能的影响
IF 1.6 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2024-08-01 Epub Date: 2024-07-12 DOI: 10.1080/00071668.2024.2332719
L A L da Silva, T C Euzébio Dornelas, F E De Marchi, T C Dos Santos

1. This study was conducted to assess the effects of different dietary omega 6:3 ratios fed to male and female Japanese quail breeders on incubation performance, chick quality and progeny performance.2. A completely randomised design was used, with five diets containing different ratios of vegetable oils rich in linoleic acid (LA from soybean oil) or α-linolenic acid (ALA from linseed oil) with LA/ALA ratios of 13.75:1, 10.69:1, 7.63:1, 4.57:1 and 1.48:1 with 12 cage replicates containing six birds each.3. There was a quadratic effect of the LA/ALA ratio on total hatchability (p < 0.011), fertile hatchability (p = 0.046) and total mortality (p = 0.046). There was no effect on fertility (p > 0.05). The LA/ALA ratios of 1.48 and 13.75 fed to both hens and cockerels or hens resulted in greater fertility, as measured by the number of days after copulation during which fertile eggs were laid and the number of points of hydrolysis on the perivitelline membrane. A decreasing linear effect (p < 0.0001) was observed on chick length and an increasing linear effect on body weight at 1 day of age. There were no effects on progeny performance.4. The LA/ALA ratio affected yolk mineral matter (p = 0.009), crude protein (p = 0.091), chick mineral matter (p < 0.038) and ether extract (p < 0.0001) contents. Maternal diet affected the fatty acid profile of egg yolk and chick liver, indicating that dietary contents were transferred to eggs and chicks.5. Fertile egg production increased with lower LA/ALA ratios. Therefore, linseed oil can be used together with soybean oil to formulate diets for female Japanese quail obtain LA/ALA ratios between 4:1 and 10:1.

1.这项研究旨在评估雌雄日本鹌鹑饲喂不同比例的欧米伽 6:3 日粮对孵化性能、雏鸟质量和后代表现的影响。采用完全随机设计,五种日粮含有不同比例的富含亚油酸(来自大豆油的 LA)或α-亚麻酸(来自亚麻籽油的α-亚麻酸)的植物油,LA/ALA 比例分别为 13.75:1、10.69:1、7.63:1、4.57:1 和 1.48:1,12 个笼子重复,每个笼子重复六只鹌鹑。LA/ALA 比率对总孵化率(p p = 0.046)和总死亡率(p = 0.046)有二次影响。对繁殖力没有影响(p > 0.05)。用 1.48 和 13.75 的 LA/ALA 比率饲喂母鸡和公鸡或母鸡可提高繁殖力,其衡量标准是交配后产可育蛋的天数和绒毛膜周围水解点的数量。线性效应递减 (p p = 0.009)、粗蛋白 (p = 0.091)、雏鸡矿物质 (p p p)
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引用次数: 0
Comparison of different derivatisation for amino acids determination of foie gras by high performance liquid chromatography (HPLC). 通过高效液相色谱法(HPLC)测定鹅肝中氨基酸的不同衍生方法比较。
IF 1.6 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2024-07-30 DOI: 10.1080/00071668.2024.2375723
R Wei, Y Teng, R Ning, Z Luo, L Bai, C Han

1. In order to compare the difference between different derivatisations for amino acids determination of foie gras via, reversed phase high performance liquid chromatography (HPLC), O-phthalaldehyde and 9-fluorenyl-methyl chloroformate (OPA-FMOC group), phenylisothiocyanate (PITC group) and 6-Aminoquinolyl-N-hydrox-ysuccinimidyl Carbamate (AQC group) were applied to derivatisation reagent in this current experiment. The determination results of automatic amino acid analyser were applied, and 17 amino acids were detected by these three derivatisation methods.2. The running times of OPA-FMOC group, PITC group and AQC group were 18, 45 and 35 min, respectively. There was a large difference between the results of OPA-FMOC group and results from the automatic amino acid analyser, although the difference between the results from PITC and the automatic amino acid analyser was minimal.3. In conclusion, the running time of OPA-FMOC group was shorter than that of PITC group and AQC group; the accuracy of the former was better than the OPA-FMOC group and AQC group for the determination of amino acid of foie gras.

为了比较不同衍生法测定鹅肝氨基酸的差异,本实验采用反相高效液相色谱法(HPLC)、邻苯二甲醛和 9-芴基甲基氯甲酸酯衍生法(OPA-FMOC 衍生法)、苯基异硫氰酸酯衍生法(PITC 衍生法)和 6-氨基喹啉-N-羟基-琥珀酰亚胺基氨基甲酸酯衍生法(AQC 衍生法)对鹅肝进行衍生。2. OPA-FMOC 组、PITC 组和 AQC 组的衍生时间分别为 18、45 和 35 分钟。总之,在测定鹅肝氨基酸时,OPA-FMOC 组的运行时间比 PITC 组和 AQC 组短;前者的准确度优于 OPA-FMOC 组和 AQC 组。
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引用次数: 0
Molecular characterisation of IBDV isolates in Turkey revealed reassortant strains. 土耳其 IBDV 分离物的分子特征发现了重交株。
IF 1.6 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2024-07-30 DOI: 10.1080/00071668.2024.2379950
G A Kurtbeyoğlu, M Akan

1. Infectious bursal disease (IBD) is an acute, highly contagious viral disease of chickens caused by a virus (IBDV) which has a bi-segmented, double-stranded RNA genome. It has five viral proteins in its structure; the VP1 gene is encoded in segment B and the other four are in segment A.2. In this study, bursae of Fabricius and spleen samples taken from chickens suspected of having clinical or subclinical IBD from a total of 50 chicken flocks located in different geographical regions of Turkey were examined.3. The RT-PCR analysis of the VP2 gene showed that 30 of the 50 samples (60%) tested positive. Eight positive isolates were chosen and RT-PCR was performed to amplify the VP1 gene.4. The study showed that reassortant field strains that cause clinical or subclinical disease are currently circulating in broiler flocks across Turkey.

1.传染性法氏囊病(IBD)是一种由病毒(IBDV)引起的鸡的急性、高度传染性病毒性疾病。它的结构中有五种病毒蛋白,VP1 基因在 B 段编码,其他四种在 A 段编码2。3. VP2 基因的 RT-PCR 分析显示,50 个样本中有 30 个(60%)呈阳性。4. 研究表明,可引起临床或亚临床疾病的野外再变种毒株目前正在土耳其各地的肉鸡群中流行。
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British Poultry Science
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