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Activity of NAD(P)-Dependent Dehydrogenases of Ovarian Vein Lymphocytes in Women with Pelvic Varicose Veins. 盆腔静脉曲张妇女卵巢静脉淋巴细胞中 NAD(P)-D 依赖性脱氢酶的活性。
IF 0.9 4区 医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL Pub Date : 2024-11-01 Epub Date: 2024-11-22 DOI: 10.1007/s10517-024-06278-7
A A Semendyaev, M A Darenskaya, D A Stupin, S I Kolesnikov, N V Semenova, D V Tukhieva, L I Kolesnikova

The activities of NAD- and NADP-dependent isocitrate dehydrogenases (NAD-ICDH and NADP-ICDH), NAD- and NADP-dependent glutamate dehydrogenases (NAD-GDH and NADP-GDH), and NAD- and NADP-dependent malate dehydrogenases (NAD-MDH and NADP-MDH) in ovarian veins lymphocytes in 183 women of reproductive age with pelvic varicose veins (PVV) and 30 women in the control group were determined. The indicators of intracellular enzymatic activity showed multidirectional changes with increasing severity of the disease. Compared to the control group, women with stage II of PVV had higher median values for lactate dehydrogenase (16.69), NAD-GDH (39.48), NADP-GDH (7.48), NADP-MDH (9.06) and lower values of NAD-ICDH (65.41). Women with stage III of PVV had lower median values for succinate dehydrogenase (9.27), NAD-MDH (26.19), and higher values for glucose-6-phosphate dehydrogenase (8.62), lactate dehydrogenase (24.67), NAD-GDH (63.81), NADP-MDH (21.05) compared to the control group. Studying the activity of NAD(P)-dependent dehydrogenases in lymphocytes at the local level during PVV allows us to evaluate the intensity and dynamics of local varicose veins progression, as well as to optimize its correction.

183 名患有盆腔静脉曲张的育龄妇女的卵巢静脉淋巴细胞中 NAD 和 NADP 依赖性异柠檬酸脱氢酶(NAD-ICDH 和 NADP-ICDH)、NAD 和 NADP 依赖性谷氨酸脱氢酶(NAD-GDH 和 NADP-GDH)以及 NAD 和 NADP 依赖性苹果酸脱氢酶(NAD-MDH 和 NADP-MDH)的活性、和 NADP-GDH)、NAD-和 NADP 依赖性谷氨酸脱氢酶(NAD-GDH 和 NADP-GDH)、NAD-和 NADP 依赖性苹果酸脱氢酶(NAD-MDH 和 NADP-MDH)。随着病情的加重,细胞内酶活性指标呈现多向变化。与对照组相比,PVV II 期妇女的乳酸脱氢酶(16.69)、NAD-GDH(39.48)、NADP-GDH(7.48)、NADP-MDH(9.06)的中值较高,而 NAD-ICDH 的中值较低(65.41)。与对照组相比,PVV III 期妇女的琥珀酸脱氢酶(9.27)、NAD-MDH(26.19)的中位值较低,而葡萄糖-6-磷酸脱氢酶(8.62)、乳酸脱氢酶(24.67)、NAD-GDH(63.81)、NADP-MDH(21.05)的中位值较高。通过研究局部静脉曲张期间淋巴细胞中依赖 NAD(P) 的脱氢酶的活性,我们可以评估局部静脉曲张发展的强度和动态,并对其矫正进行优化。
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引用次数: 0
Impaired Communication through Gap Junctions Reduces the Angiogenic Potential of the Secretome in Mesenchymal Stromal Cell-Endothelial Cell Interactions In Vitro. 间隙连接通信受损会降低间充质基质细胞-内皮细胞体外相互作用中分泌组的血管生成潜能
IF 0.9 4区 医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL Pub Date : 2024-11-01 Epub Date: 2024-11-23 DOI: 10.1007/s10517-024-06296-5
M I Ezdakova, E R Andreeva

Highly specialized gap junctions play an important role in the interaction between endothelial (EC) and multipotent mesenchymal stromal cells (MSC). Inhibition of gap junctions with a specific inhibitor carbenoxolone attenuates the effects of the medium conditioned by MSC-EC co-culture on proliferation and migration of cultured EC. In conditioned medium from co-culture, the levels of angiogenic mediators (VEGF-A, FGF-2, MCP-1, etc.) were decreased, which apparently determines lower angiogenic effect of the conditioned medium on the growth of the vascular network in the chorioallantois membrane of quail embryo in ovo. Suppression of communication through gap junctions in associations of MSC and EC, the structural and functional units of physiological and reparative angiogenesis, can directly reduce the level of proangiogenic mediators in the microenvironment, which, in turn, can help to control the regulation of vascular function in pathologies.

高度特化的间隙连接在内皮细胞(EC)和多能间充质基质细胞(MSC)之间的相互作用中发挥着重要作用。用特异性抑制剂羧甲唑酮抑制间隙连接可减轻间充质干细胞-内皮细胞共培养的条件培养基对培养的内皮细胞增殖和迁移的影响。在共培养的条件培养基中,血管生成介质(VEGF-A、FGF-2、MCP-1 等)的水平降低,这显然决定了条件培养基对卵内鹌鹑胚胎绒毛膜血管网络生长的血管生成作用降低。间充质干细胞和血管内皮细胞是生理性和修复性血管生成的结构和功能单位,抑制间隙连接在间充质干细胞和血管内皮细胞结合过程中的通讯,可直接降低微环境中促血管生成介质的水平,进而有助于控制病变血管功能的调节。
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引用次数: 0
The Effect of Transplantation of Ensheathing Cells of the Olfactory Mucosa into the Hippocampal Area on the Restoration of Cognitive Abilities in Rats with Experimental Alzheimer's Disease. 将嗅粘膜内鞘细胞移植到海马区对恢复实验性阿尔茨海默病大鼠认知能力的影响
IF 0.9 4区 医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL Pub Date : 2024-11-01 Epub Date: 2024-11-23 DOI: 10.1007/s10517-024-06293-8
E K Karsuntseva, A D Voronova, S S Andretsova, V S Shishkina, A V Chadin, G A Fursa, A V Fedorov, I V Reshetov, O V Stepanova, V P Chekhonin

Alzheimer's disease was induced in female Wistar rats by bilateral injection of β-amyloid fragment 1-42 into the hippocampal region. After 8 weeks, ensheathing cells of the olfactory mucosa were transplanted into the hippocampus at the same stereotactic coordinates. These cells survived for 8 weeks; large clusters of cells were observed on week 4. On weeks 3-5 after transplantation of ensheathing cells, experimental animals demonstrated a significant cognitive improvement (memory and spatial orientation). The obtained results create prerequisites for further studies of ensheathing cells as a potential cell product for personalized therapy of Alzheimer's disease.

向雌性Wistar大鼠的海马区双侧注射β-淀粉样蛋白片段1-42,诱发阿尔茨海默病。8 周后,嗅粘膜的鞘状细胞被移植到相同立体定向坐标的海马区。这些细胞存活了8周,在第4周时观察到大的细胞簇。在移植鞘状细胞后的第 3-5 周,实验动物的认知能力(记忆力和空间定向能力)明显提高。这些结果为进一步研究作为阿尔茨海默病个性化治疗潜在细胞产品的鞘状细胞创造了先决条件。
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引用次数: 0
Effect of Experimental Litter Reduction on Cerebellum Development in Suckling Rats. 实验性减窝对乳鼠小脑发育的影响
IF 0.9 4区 医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL Pub Date : 2024-10-01 Epub Date: 2024-10-26 DOI: 10.1007/s10517-024-06270-1
B Ya Ryzhavskii, A V Lanshakova, Yu B Malofey

We studied morphological features of the cerebellum in 14-day-old Wistar rats from reduced litters (the number of pups was reduced from 10-12 to 6 on the next day after birth). The control group comprised 14-day-old animals from litters of medium size (10-12 rat pups). Rats from reduced litters had greater body weight and brain weight. The weight of the cerebellum, together with the weight of the adjacent part of the brain stem and the thickness of cerebellar cortex also significantly exceeded the corresponding parameters in control animals. The thickness of the molecular layer of the cerebellar cortex and the numerical density of Purkinje cells in these rats did not differ significantly from the control. The thickness of the external granular (neurogenic) layer of the cerebellar cortex in rats from reduced litters was smaller. This can indicate accelerated reduction of this layer that persists in rats until days 20-22 of age. Numerical density of cells in the external granular layer of control and experimental animals was similar. Numerical density Ki-67+ cells in this layer, as well as GFAP+ glial cells in the granular layer of the cerebellar cortex in rats from reduced litters significantly exceeded the corresponding parameters in control animals. The cerebellum of rats from litters reduced 1 day after birth had a number of differences in important indicators reflecting the rate of its development during the neonatal and suckling periods of ontogeny.

我们研究了产仔数减少(出生后第二天幼鼠数从 10-12 只减少到 6 只)的 14 天大 Wistar 大鼠的小脑形态特征。对照组包括产仔数中等(10-12 只幼鼠)的 14 天大的大鼠。产仔数减少的大鼠体重和脑重都较大。小脑的重量、脑干邻近部分的重量和小脑皮层的厚度也明显超过对照组动物的相应参数。这些大鼠的小脑皮质分子层厚度和浦肯野细胞的数量密度与对照组没有显著差异。减胎大鼠的小脑皮质外颗粒层(神经源)厚度较小。这表明小脑皮质外颗粒层的厚度在大鼠20-22日龄之前一直在加速减少。对照组和实验组动物外颗粒层的细胞数量密度相似。减胎大鼠小脑皮层颗粒层中Ki-67+细胞的数量密度和GFAP+胶质细胞的数量密度明显超过对照组动物的相应参数。出生后1天减胎仔鼠的小脑在一些重要指标上存在差异,这些指标反映了小脑在新生儿期和哺乳期的发育速度。
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引用次数: 0
Identification of Giant Isoforms of Obscurin in Rat Striated Muscles Using Polyclonal Antibodies. 使用多克隆抗体鉴定大鼠横纹肌中的 Obscurin 巨型异构体
IF 0.9 4区 医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL Pub Date : 2024-10-01 Epub Date: 2024-10-23 DOI: 10.1007/s10517-024-06259-w
Y V Gritsyna, V K Zhalimov, T A Uryupina, A D Ulanova, A G Bobylev, I M Vikhlyantsev

Using produced polyclonal antibodies specific to the N-terminal sequence (residues 61-298) of rat obscurin, we investigated the isoform composition of this protein in 4 striated muscles: myocardium of the left ventricle, diaphragm, skeletal m. gastrocnemius (containing mainly fast fibers), and m. soleus (containing mainly slow fibers). The m. gastrocnemius, m. soleus, and diaphragm were found to have 2 giant isoforms of obscurin: a smaller A-isoform and a larger B-isoform. Their molecular weights were ~870 and ~1150 kDa in the diaphragm and m. gastrocnemius and ~880 and ~1130 kDa in m. soleus, respectively. The B-isoform to A-isoform ratio was 1:3 in the diaphragm and m. soleus and 1:4 in the m. gastrocnemius. In the left-ventricular myocardium, A-isoform of obscurin with a molecular weight of ~880 kDa was found. No other obscurin isoforms or their fragments within the molecular weight range of 10 up to ~800 kDa were revealed in the investigated rat striated muscles. The antibodies produced are recommended for research into qualitative and quantitative changes of giant obscurin isoforms in rat striated muscles in the norm and during the development of pathological processes.

我们使用特异于大鼠淫羊藿苷N-末端序列(残基61-298)的多克隆抗体,研究了4种横纹肌中淫羊藿苷的异构体组成:左心室心肌、膈肌、骨骼肌腓肠肌(主要包含快肌纤维)和比目鱼肌(主要包含慢肌纤维)。研究发现,腓肠肌、比目鱼肌和膈肌含有两种巨型异构体:较小的A-异构体和较大的B-异构体。它们的分子量在膈肌和腓肠肌中分别为 ~870 和 ~1150 kDa,在比目鱼肌中分别为 ~880 和 ~1130 kDa。在膈肌和比目鱼肌中,B-异构体与 A-异构体之比为 1:3,在腓肠肌中为 1:4。在左心室心肌中发现了分子量约为 880 kDa 的淫羊藿苷 A-异构体。在调查的大鼠横纹肌中,没有发现分子量在 10 至 ~800 kDa 范围内的其他淫羊藿苷异构体或其片段。该抗体可用于研究大鼠横纹肌中巨型淫羊藿苷异构体在正常和病理过程中的定性和定量变化。
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引用次数: 0
Metabolic Syndrome in Reproductive Age Women of Various Ethnic Groups. Neuroendocrine Status and Lipid Peroxidation System. 不同种族育龄妇女的代谢综合征。神经内分泌状态和脂质过氧化系统。
IF 0.9 4区 医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL Pub Date : 2024-10-01 Epub Date: 2024-10-22 DOI: 10.1007/s10517-024-06254-1
L V Belenkaya, M A Darenskaya, S I Kolesnikov, L F Sholokhov, I N Danusevich, L M Lazareva, Ia G Nadeliaeva, L V Suturina, L I Kolesnikova

We analyzed the state of neuroendocrine regulation and LPO-antioxidant defense systems in reproductive age women with metabolic syndrome (MetS), representatives of the Russian and Buryat ethnic groups. Compared to the corresponding control groups, women from the Russian ethnic group with MetS had elevated levels of thyroid-stimulating hormone and free androgen index (FAI) and reduced levels of sex hormone-binding globulin, while women from the Buryat ethnic group had increased levels of prolactin and FAI. Changes in the LPO system in women of the Russian ethnic group with MetS consisted in an increase in the levels of substrates with double bonds, TBA-reactive substances, and fat-soluble vitamins. Buryat women with MetS had a higher content of primary oxidation products and reduced levels of glutathione. The results of the study indicate a hyperandrogenic shift in the neuroendocrine regulation system, as well as compensatory influences from different parts of the antioxidant defense system in women of reproductive age with MetS, depending on their ethnicity. These findings indicate the need for assessing and monitoring the levels of these metabolites in women with MetS, considering their ethnicity.

我们分析了患有代谢综合征(MetS)的俄罗斯族和布里亚特族育龄妇女的神经内分泌调节和LPO-抗氧化防御系统的状况。与相应的对照组相比,患有代谢综合征的俄罗斯族妇女的促甲状腺激素和游离雄激素指数(FAI)水平升高,性激素结合球蛋白水平降低,而布里亚特族妇女的催乳素和FAI水平升高。患有 MetS 的俄罗斯族妇女体内 LPO 系统的变化包括双键底物、TBA 反应物质和脂溶性维生素含量的增加。患有 MetS 的布里亚特妇女的初级氧化产物含量较高,谷胱甘肽水平降低。研究结果表明,患有 MetS 的育龄妇女的神经内分泌调节系统出现了高雄激素转变,抗氧化防御系统的不同部分也产生了补偿性影响,这取决于她们的种族。这些研究结果表明,有必要评估和监测 MetS 女性代谢物的水平,同时考虑到她们的种族。
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引用次数: 0
Effect of Lead, Cadmium, and Their Combination on the Heart Rate Regulation in Nonlinear Rats. 铅、镉及其组合对非线性大鼠心率调节的影响
IF 0.9 4区 医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL Pub Date : 2024-10-01 Epub Date: 2024-10-23 DOI: 10.1007/s10517-024-06271-0
A V Tryasuchev, V O Stupin, E V Kuryanova

We studied heart rate variability (HRV) and the concentrations of heavy metals in the heart tissue after 14-fold peroral administration of heavy metal salts: lead acetate (50 mg/kg), cadmium chloride (5 mg/kg), and their combination. After administration of lead, its concentration in the myocardium exceeded the control values by 1.2 times or did not change in case of combination with cadmium; after administration of cadmium, its concentration in the myocardium exceeded the control value by 6.1 times or by 2 times after combined administration. After lead administration, a moderate increase in HR, low values of stress and centralization indices, and an increase in the power in all ranges of the HRV spectrum were recorded. After cadmium intoxication, a tendency to a decrease in heart rhythm variability, a moderate increase in HR, stress and centralization indices, and an increase in the contribution of VLF waves in the HRV spectrum were observed. After combined administration, a pronounced increase in HR and stress index and a moderate increase in the centralization of heart rhythm control were recorded, which was similar to changes in HRV observed after administration of cadmium alone. In the experiment, the concentration of cadmium in the myocardium increased to a much greater extent than the concentration of lead. After lead administration, the autonomous type of regulation persisted and parasympathetic influences increased. Cadmium apparently acts as a stress factor and increases the role of sympathoadrenal influences and central ergotropic structures in the heart rhythm regulation.

我们研究了口服重金属盐(醋酸铅(50 毫克/千克)、氯化镉(5 毫克/千克)和它们的组合)14 倍后的心率变异性和心脏组织中的重金属浓度。给药后,铅在心肌中的浓度比对照值高出 1.2 倍,或与镉混合使用时没有变化;给药后,镉在心肌中的浓度比对照值高出 6.1 倍,或与镉混合使用时高出 2 倍。服用铅后,记录到心率适度增加,应激指数和集中指数值较低,心率变异频谱所有范围的功率都有所增加。镉中毒后,心律变异性呈下降趋势,心率、应激和集中指数适度增加,心率变异频谱中 VLF 波的贡献增加。联合给药后,记录到心率和压力指数明显增加,心律控制的集中程度适度增加,这与单独给镉后观察到的心率变异性变化相似。在实验中,心肌中镉浓度的增加幅度远大于铅浓度的增加幅度。用铅后,自主调节类型持续存在,副交感神经的影响增加。镉显然是一种应激因素,增加了交感肾上腺影响和中枢麦角结构在心律调节中的作用。
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引用次数: 0
Expression of Markers Associated with Epithelial-Mesenchymal Transition and Extracellular Matrix Degradation in Human Uveal Melanoma. 人葡萄膜黑色素瘤中上皮-间质转化和细胞外基质降解相关标记物的表达
IF 0.9 4区 医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL Pub Date : 2024-10-01 Epub Date: 2024-10-23 DOI: 10.1007/s10517-024-06266-x
A Yu Shatruk, N P Bgatova, A V Yeremina, A N Trunov, V V Chernykh, Iu S Taskaeva

The expression of markers associated with epithelial-mesenchymal transition (EMT) and extracellular matrix degradation in human uveal melanoma tissue samples and postequatorial zone of the choroid was assessed by immunohistochemical staining. Increased expression of EMT markers E-cadherin and vimentin was observed in the tumor. The ratio of MMP-9 to TIMP-1 proteins related to the extracellular matrix degradation was higher in the tumor. These results may indicate activation of EMT-like process in the uveal melanoma cells and degradation of the extracellular matrix, which can contribute to the development of collective invasion in uveal melanoma.

免疫组化染色法评估了人葡萄膜黑色素瘤组织样本和脉络膜后赤道区中上皮-间质转化(EMT)和细胞外基质降解相关标记物的表达情况。在肿瘤中观察到 EMT 标志物 E-cadherin 和波形蛋白的表达增加。肿瘤中与细胞外基质降解有关的 MMP-9 和 TIMP-1 蛋白的比例较高。这些结果可能表明,葡萄膜黑色素瘤细胞中的EMT样过程被激活,细胞外基质被降解,这可能会导致葡萄膜黑色素瘤发生集体侵袭。
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引用次数: 0
Changes in the Tissue Barrier after Exposure to Lipopolysaccharide on the Apical Side of Enterocytes and the Follicle-Associated Epithelium in Peyer's Patches of the Rat Intestine. 暴露于脂多糖后大鼠肠道佩耶氏斑的肠细胞顶端和滤泡相关上皮细胞的组织屏障的变化
IF 0.9 4区 医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL Pub Date : 2024-10-01 Epub Date: 2024-10-31 DOI: 10.1007/s10517-024-06263-0
A A Fedorova, O V Rybalchenko, L S Okorokova, V V Kapustina, O G Orlova, A G Markov

To study the para- and transcellular permeability of columnar epithelium and follicle-associated epithelium of Peyer's patches in the rat intestine, LPS was applied from the mucosal side to simulate the action of endotoxins from gram-negative bacteria of gut microbiota. LPS did not affect transepithelial resistance or sodium fluorescein permeability, but increased the levels of claudin-3 and claudin-4 in enterocytes, suggesting strengthening of the paracellular intestinal barrier. Transcellular permeability was evaluated by electron microscopy based on the number of vesicular structures in the cytoplasm of different cell types. LPS increased the number of small vesicles in follicle-associated epithelium of Peyers' patches. In columnar epithelial cells, LPS reduced the number of smaller vesicles and increased the number of larger ones. LPS did not damage the tissue barrier, but enhanced transcytosis, which could potentiate the effects of endotoxin on its receptors in the intestinal mucosa.

为了研究大鼠肠道佩耶氏斑的柱状上皮和滤泡相关上皮的细胞旁通透性和跨细胞通透性,从粘膜侧施加 LPS 以模拟肠道微生物群革兰氏阴性菌内毒素的作用。LPS 不影响经上皮阻力或荧光素钠通透性,但增加了肠细胞中 claudin-3 和 claudin-4 的水平,表明细胞旁肠屏障得到了加强。根据不同类型细胞胞质中囊泡结构的数量,通过电子显微镜对跨细胞渗透性进行了评估。LPS 增加了佩尔氏斑的滤泡相关上皮细胞中的小囊泡数量。在柱状上皮细胞中,LPS 减少了小囊泡的数量,增加了大囊泡的数量。LPS 不会破坏组织屏障,但会增强转囊作用,从而增强内毒素对肠粘膜受体的影响。
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引用次数: 0
Nitrogen Oxide (NO) in the Pathogenesis of Preeclampsia. 子痫前期发病机制中的氧化氮(NO)。
IF 0.9 4区 医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL Pub Date : 2024-10-01 Epub Date: 2024-10-22 DOI: 10.1007/s10517-024-06255-0
V Yu Titov, A N Osipov, I V Babenkova, Yu O Teselkin, R I Shalina, Yu V Vykhristyuk, S Yu Lebedeva

The concentrations of nitric oxide (NO) donors in the plasma of pregnant women with preeclampsia is several times higher than in healthy pregnant women. Antihypertensive drugs acting not through the NO-mediated mechanisms normalized BP in some women with preeclampsia, but did not significantly reduce the levels of NO donors in the plasma. It appears that preeclampsia is associated with insufficient NO availability for the targets, rather than low intensity of NO synthesis. The concentration of NO donor molecules in the plasma can therefore be a useful additional diagnostic marker of preeclampsia.

子痫前期孕妇血浆中一氧化氮(NO)供体的浓度是健康孕妇的数倍。非通过一氧化氮介导机制发挥作用的降压药可使一些子痫前期妇女的血压恢复正常,但并不能显著降低血浆中一氧化氮供体的水平。看来,子痫前期与靶点的 NO 供应不足有关,而不是与 NO 合成强度低有关。因此,血浆中 NO 供体分子的浓度可以作为子痫前期的一个有用的额外诊断指标。
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引用次数: 0
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