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Snow algal blooms observed in Mt. Echigo-Komagatake, Niigata prefecture, Japan(新潟県の越後駒ヶ岳で観察された雪氷藻類の大繁殖) Snow algal blooms observed in Mt. Echigo-Komagatake, Niigata prefecture,Japan(在新潟县的越后驹岳观察到的冰雪藻类的大繁殖)
Q4 GEOGRAPHY, PHYSICAL Pub Date : 2023-01-01 DOI: 10.5331/bgr.23r01
Suzunosuke USUBA, Tsubasa TAKAHASHI, Nozomu TAKEUCHI
Snow algae are photosynthetic microbes growing on snowpacks and are commonly observed in mountain areas in Japan. In this study, we report the snow algal blooms observed in Mt. Echigo-Komagatake located in Niigata prefecture, Japan. Field investigation was conducted in late June of 2021 on a snow patch located in an alpine zone at an altitude of 2000 m above sea level. Patches of snow with red, green, or brown color were observed on the snowpack. Microscopic observation showed that the colored snow contained various morpho-types of snow algal cells, which appeared to be similar to those previously reported in Japan. The algal communities of the colored snow were mostly dominated by red spherical cells, which is likely to be Sanguina nivaloides, and consistent with those of the red snow reported in other alpine snowpacks in Japan. However, some samples showed a community consisting of various cell types, including multiple species of genus Chloromonas. The difference in community structure may be due to distance from vegetation around the snow patch, nutrient conditions of the snow, and/or growth period of the algae. The snow algal blooms of Mt. Echigo-Komagatake can be characterized by the appearance of various colors and algal communities on a snowpack.
雪藻是生长在积雪上的光合微生物,在日本的山区很常见。在这项研究中,我们报告了位于日本新泻县的越后komagatake山的雪藻华。野外调查于2021年6月下旬在海拔2000米的高寒地区进行。在积雪上可以观察到红色、绿色或棕色的雪块。显微镜观察发现,彩色雪中含有多种形态类型的雪藻细胞,与日本先前报道的雪藻细胞相似。彩色雪的藻类群落以红色球形细胞为主,可能为三血藻(Sanguina nivaloides),与日本其他高山积雪的红色雪一致。然而,一些样品显示一个由多种细胞类型组成的群落,包括多种绿单胞菌属。群落结构的差异可能与雪斑周围植被的距离、雪的营养条件和/或藻类的生长期有关。越后- komagatake山的雪藻华可以以各种颜色的外观和积雪上的藻类群落为特征。
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引用次数: 0
Assessments of long-term precipitation and temperature variations during snow cover periods using high-elevation AMeDAS data in central Japan 利用日本中部高海拔AMeDAS数据评估积雪期长期降水和温度变化
IF 1 Q4 GEOGRAPHY, PHYSICAL Pub Date : 2023-01-01 DOI: 10.5331/bgr.22a01
K. Ueno
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引用次数: 0
Atmosphere-sea ice-ocean interaction study in Saroma-ko Lagoon, Hokkaido, Japan 2021 日本北海道Saroma-ko泻湖大气-海-冰-海相互作用研究2021
IF 1 Q4 GEOGRAPHY, PHYSICAL Pub Date : 2022-01-01 DOI: 10.5331/bgr.21r02
Daiki Nomura, H. Ikawa, Y. Kawaguchi, N. Kanna, T. Kawakami, Yuichi Nosaka, S. Umezawa, Manami Tozawa, Takahito Horikawa, Reishi Sahashi, Taichi Noshiro, Ibuki Kaba, Makoto Ozaki, F. Kondo, K. Ono, I. Yabe, E. Y. Son, T. Toyoda, S. Kameyama, Changqing Wang, H. Obata, A. Ooki, H. Ueno, A. Kasai
An atmosphere-sea ice-ocean interaction study was conducted at the Saroma-ko Lagoon, Hokkaido, Japan from 27 February to 9 March 2021. Air-sea ice CO2 flux measurements were conducted by eddy covariance and chamber methods together with meteorological observations. Sea ice cores were collected to understand the interaction between the atmosphere and under-ice water with respect to fluxes of heat and biogeochemical components. Physical and biogeochemical parameters such as temperature, salinity, ice structure, environmental DNA, and concentrations of gases, nutrients, and trace metals were measured. Under-ice water properties were monitored to quantify heat budgets and interactions with sea ice biogeochemical properties. Incubation experiments with ice algae were conducted. Equipment such as airsea ice CO2/CH4 flux chambers, an eddy covariance system, a trace metal analyzer, and a pump and sampler for environmental DNA were tested and compared to prepare for future Arctic and Antarctic expeditions. In addition, movies and pictures were taken under the auspices of the educational program of Hokkaido University (LASBOS) to enhance educational programs in ocean science. The field campaign activities provided useful information for inter-comparison research and future studies of sea ice in polar oceans.
于2021年2月27日至3月9日在日本北海道Saroma-ko泻湖进行了一项大气-海-冰-海相互作用研究。利用涡旋相关法和气室法结合气象观测资料对海气冰CO2通量进行了测量。收集海冰核是为了了解大气和冰下水在热通量和生物地球化学成分方面的相互作用。测量了物理和生物地球化学参数,如温度、盐度、冰结构、环境DNA以及气体、营养物质和微量金属的浓度。监测冰下水的性质,量化热收支及其与海冰生物地球化学性质的相互作用。对冰藻进行了孵育实验。对空气-海冰CO2/CH4通量室、涡流相关系统、痕量金属分析仪、环境DNA泵和采样器等设备进行了测试和比较,为未来的北极和南极探险做准备。此外,还在北海道大学(LASBOS)的教育计划的支持下拍摄了电影和照片,以加强海洋科学的教育计划。实地运动活动为相互比较研究和今后对极地海洋海冰的研究提供了有用的资料。
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引用次数: 1
Ice Core Drilling and the Related Observations at SE-Dome site, southeastern Greenland Ice Sheet 格陵兰岛冰盖东南部SE-Dome冰芯钻探及相关观测
IF 1 Q4 GEOGRAPHY, PHYSICAL Pub Date : 2021-01-01 DOI: 10.5331/bgr.21r01
Y. Iizuka, S. Matoba, M. Minowa, Tetsuhide Yamasaki, K. Kawakami, Ayako Kakugo, Morihiro Miyahara, A. Hashimoto, M. Niwano, T. Tanikawa, K. Fujita, T. Aoki
In order to construct reliable deposited-aerosol database on the Anthropocene (from 1850 to 2020), we obtained a 250-meter-long ice core from the Southeastern Greenland Dome on May and June 2021, where is one of the highest accumulation domes in Greenland. The age of the ice core at a depth of 250 m was roughly estimated to be AD 1827 based on the timescale from a previously analyzed shallower ice core. The age of the sampled ice core satisfied the prerequisite conditions for constructing aerosol deposition database for Anthropocene. In addition, surface elevation, borehole temperatures, and internal stratigraphy of the ice sheet were performed, and meteorological and snow-pit observations were also conducted. Furthermore, we sampled aerosol and snow from the ice sheet for chemical and physical analyses.
为了构建可靠的人类世(1850 - 2020)沉积气溶胶数据库,我们于2021年5月和6月在格陵兰岛最高堆积圆顶之一的格陵兰东南部获得了一个250米长的冰芯。根据先前分析的较浅冰芯的时间尺度,大致估计250 m深度冰芯的年龄为公元1827年。取样冰芯的年龄满足建立人类世气溶胶沉积数据库的前提条件。此外,还进行了地表高程、钻孔温度、冰盖内部地层、气象和雪坑观测。此外,我们从冰盖上取样气溶胶和雪进行化学和物理分析。
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引用次数: 0
Saroma-ko Lagoon Observations for sea ice Physico-chemistry and Ecosystems 2019 (SLOPE2019) 2019年Saroma-ko泻湖海冰物理化学和生态系统观测(SLOPE2019)
IF 1 Q4 GEOGRAPHY, PHYSICAL Pub Date : 2020-03-31 DOI: 10.5331/bgr.19r02
Daiki Nomura, P. Wongpan, T. Toyota, T. Tanikawa, Y. Kawaguchi, Takashi Ono, Tomoni Ishino, Manami Tozawa, Tetsuya P. Tamura, I. Yabe, E. Y. Son, F. Vivier, A. Lourenço, Marion Lebrun, Yuichi Nosaka, T. Hirawake, Atshushi Ooki, S. Aoki, B. Else, F. Fripiat, J. Inoue, M. Vancoppenolle
Saroma-ko Lagoon, located on the Okhotsk Sea coast of Hokkaido, is seasonally covered by flat, homogeneous, easily accessible and safe sea ice. As such, it proves a very useful experimental site for the study of sea ice processes, the inter-comparison of methods, the testing of equipment and the training to polar regions. In this contribution, we describe the a physical, chemical, and ecosystem survey at Saroma-ko Lagoon, conducted over February 23–28, 2019 under the auspices of the SLOPE2019 (Saroma-ko Lagoon Observations for sea ice Physico-chemistry and Ecosystems 2019) program. Sea ice cores were collected to examine temperature, salinity, oxygen isotopic ratio, thin sections, and chemical and biological parameters such as carbonate chemistry, CH 4 , nutrients, chlorophyll a concentrations, and ice algae community assemblage. Broadband and spectral irradiance measurements were carried out above/under the sea ice, and different sensors were inter-compared at close positions and environments. Equipment such as spectrometers, air–sea ice CO 2 /CH 4 flux chamber, and under-ice turbulent heat flux systems were tested for future Arctic and Antarctic expeditions. Finally, an artificial pool was dug into the sea ice to understand the effect of snow particles on ice growth and to compare the gas exchange process over sea ice with an ice-free water surface. Our SLOPE2019 field campaign activities provided useful information for inter-comparison work and future sea ice research in the polar oceans.
Saroma-ko泻湖位于北海道鄂霍次克海岸,季节性地被平坦、均匀、容易接近和安全的海冰覆盖。因此,它被证明是一个非常有用的实验场所,海冰过程的研究,方法的相互比较,设备的测试和极地地区的培训。在这篇文章中,我们描述了2019年2月23日至28日在SLOPE2019 (Saroma-ko Lagoon海冰物理化学和生态系统观测2019)计划的主持下,在Saroma-ko泻湖进行的物理、化学和生态系统调查。收集海冰岩芯,检测温度、盐度、氧同位素比、薄片以及碳酸盐化学、ch4、营养物质、叶绿素a浓度和冰藻群落组合等化学和生物参数。在海冰上/海冰下进行了宽带和光谱辐照度测量,并在近距离和环境下对不同传感器进行了相互比较。光谱仪、空气-海冰CO 2 /CH 4通量室和冰下湍流热通量系统等设备为未来的北极和南极探险进行了测试。最后,在海冰中挖了一个人工水池,以了解雪颗粒对冰生长的影响,并将海冰上的气体交换过程与无冰水面进行比较。我们的SLOPE2019野外活动为极地海洋的相互比较工作和未来的海冰研究提供了有用的信息。
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引用次数: 5
GLOFs of Laguna de los Témpanos, glacier-dammed side lake of Glaciar Steffen, Hielo Patagónico Norte, Chile, since 1974 拉古纳德洛斯特姆帕诺斯,斯特芬冰川侧湖,海洛Patagónico北,智利,自1974年
IF 1 Q4 GEOGRAPHY, PHYSICAL Pub Date : 2020-01-01 DOI: 10.5331/bgr.20r01
M. Aniya, Alejandro Dusaillant, J. O'kuinghttons, G. Barcaza, Sidi Bravo
Glacier lake outburst floods (GLOF) at Laguna de los Témpanos, a glacier-dammed side lake of Glaciar Steffen, Hielo Patagónico Norte, were documented for a period between December 1974 and February 2020. With manual interpretation of 150 remote sensing images of aerial surveys, vertical aerial photographs, Landsat MSS, TM, ETM and OLI, ALOS, and ASTER images, 19 GLOFs were captured/inferred by focusing on icebergs and water levels, except two periods in the 1980 s and the 1990 s for which no image was available. This translates to the occurrence of the GLOF on average once ca. every 14 months. Many GLOFs occurred in late summer to early fall, with a few in late spring: but one GLOF was inferred to have occurred in wintertime. The causes of GLOFs were supposed to be heavy rainfalls, probably accompanied with rapid snow/ice melting by warm air temperatures, judging from the general weather condition over the laguna area. The latest two GLOFs (2016 & 2017) were very large, enough to have completely exposed the lake floor in the middle section. The GLOF of 2017 (Mar. 31) was registered in a hydrograph set up at Lower Río Huemules. After this GLOF, the water level has become stable with a more or less continuous outlet stream along the glacier sidewall and there has been no GLOF to date. So probably the prospect of another GLOF has considerably diminished by now. As Glaciar Steffen receded about 6 km during this study period, the glacier has thinned accordingly, to which the water level adjusted with three distinctive relatively stable states while fluctuating frequently.
1974年12月至2020年2月期间,研究人员记录了拉古纳德洛斯特姆帕诺斯冰川湖爆发洪水(GLOF),拉古纳德洛斯特姆帕诺斯冰川湖是位于Patagónico北部的斯特芬冰川的一个冰川大坝侧湖。通过人工解译150幅遥感图像,包括航空调查、垂直航空照片、Landsat MSS、TM、ETM和OLI、ALOS和ASTER图像,除了20世纪80年代和90年代两个没有图像的时期外,通过聚焦冰山和水位捕获/推断出了19幅GLOFs。这意味着GLOF平均每14个月发生一次。许多GLOF发生在夏末至初秋,少数发生在晚春,但据推测,有一次GLOF发生在冬季。从拉古纳地区的一般天气状况来看,GLOFs的原因应该是暴雨,可能伴随着温暖的空气温度导致的冰雪快速融化。最近的两次GLOFs(2016年和2017年)非常大,足以完全暴露中段的湖底。2017年(3月31日)的GLOF在Lower Río Huemules设立的水道中注册。此后,水位趋于稳定,沿冰川侧壁有一条或多或少连续的出水口流,迄今为止没有发生过GLOF。因此,到目前为止,另一次全球大萧条的前景可能已经大大减弱。随着Steffen冰川在本研究期间后退约6 km,冰川随之变薄,水位在频繁波动的同时以三种不同的相对稳定状态进行调整。
{"title":"GLOFs of Laguna de los Témpanos, glacier-dammed side lake of Glaciar Steffen, Hielo Patagónico Norte, Chile, since 1974","authors":"M. Aniya, Alejandro Dusaillant, J. O'kuinghttons, G. Barcaza, Sidi Bravo","doi":"10.5331/bgr.20r01","DOIUrl":"https://doi.org/10.5331/bgr.20r01","url":null,"abstract":"Glacier lake outburst floods (GLOF) at Laguna de los Témpanos, a glacier-dammed side lake of Glaciar Steffen, Hielo Patagónico Norte, were documented for a period between December 1974 and February 2020. With manual interpretation of 150 remote sensing images of aerial surveys, vertical aerial photographs, Landsat MSS, TM, ETM and OLI, ALOS, and ASTER images, 19 GLOFs were captured/inferred by focusing on icebergs and water levels, except two periods in the 1980 s and the 1990 s for which no image was available. This translates to the occurrence of the GLOF on average once ca. every 14 months. Many GLOFs occurred in late summer to early fall, with a few in late spring: but one GLOF was inferred to have occurred in wintertime. The causes of GLOFs were supposed to be heavy rainfalls, probably accompanied with rapid snow/ice melting by warm air temperatures, judging from the general weather condition over the laguna area. The latest two GLOFs (2016 & 2017) were very large, enough to have completely exposed the lake floor in the middle section. The GLOF of 2017 (Mar. 31) was registered in a hydrograph set up at Lower Río Huemules. After this GLOF, the water level has become stable with a more or less continuous outlet stream along the glacier sidewall and there has been no GLOF to date. So probably the prospect of another GLOF has considerably diminished by now. As Glaciar Steffen receded about 6 km during this study period, the glacier has thinned accordingly, to which the water level adjusted with three distinctive relatively stable states while fluctuating frequently.","PeriodicalId":9345,"journal":{"name":"Bulletin of glaciological research","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71026690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Monitoring bending stress of trees during a snowy period using strain gauges 利用应变计监测降雪期间树木的弯曲应力
IF 1 Q4 GEOGRAPHY, PHYSICAL Pub Date : 2020-01-01 DOI: 10.5331/bgr.20a01
A. Miyashita, Ryoko Minamino, Koichiro Sawakami, Takafumi Katsushima, M. Tateno
The load created by snow greatly affects forest trees; however, almost no studies have reported on the mechanical stress in tree trunks due to snow under field conditions. In this study, we monitored trunk bending stresses using strain gauges during the snowfall season at two sites, Tadami and Kaneyama, both in a cool-temperate forest in Japan. Measurements were made of the various sizes and trunk shapes of beech trees at Tadami, and of beech trees and Japanese cedars at Kaneyama. Young’s modulus and bending strength were also measured. At Tadami, beech trees with substantially curved trunks often bent down to the ground surface soon after snowfall started. The strain no longer increased once a tree had lodged; however, individuals with larger diameters had stresses exceeding the proportional limit stress. By contrast, among beech trees with erect trunks, those with larger diameters had smaller maximum strain and estimated stress values. These results imply that in areas greatly affected by snow, beech trees struggle both to remain erect and to grow to diameters of 10 cm or more. At Kaneyama, large strain values exceeding 1 % were observed in trees with a diameter at breast height ≤7 cm, all of which were cedars. Among trees of the same diameter, strain values were lower in beech trees than in cedars, and Young’s modulus was three times larger for beech trees. These results indicate that it is more difficult to grow erect cedars compared to erect beech trees in regions with deep snow.
积雪产生的负荷对森林树木的影响很大;然而,在野外条件下,几乎没有关于积雪对树干机械应力的研究报道。在这项研究中,我们在日本寒温带森林的两个地点,Tadami和Kaneyama,使用应变仪监测了降雪季节树干弯曲应力。测量了忠美山毛榉和金山山毛榉和日本雪松的不同大小和树干形状。测定了杨氏模量和抗弯强度。在忠美,树干弯曲的山毛榉树经常在降雪开始后不久就弯到地面上。一旦一棵树倒下,菌株就不再增加;然而,直径较大的个体应力超过比例极限应力。而树干直立的山毛榉,直径越大,最大应变和估计应力值越小。这些结果表明,在受雪影响很大的地区,山毛榉树既要保持直立,又要长到直径10厘米或更大。在山山,在胸径≤7 cm的树木中观察到大于1%的大应变值,这些树木都是雪松。在相同直径的树木中,山毛榉的应变值低于雪松,杨氏模量是山毛榉的3倍。这些结果表明,在积雪较深的地区,直立的雪松比直立的山毛榉更难生长。
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引用次数: 0
Concentrations of polycyclic aromatic hydrocarbons in Antarctic snow polluted by research activities using snow mobiles and diesel electric generators 利用雪地摩托和柴油发电机的研究活动污染的南极雪中多环芳烃的浓度
IF 1 Q4 GEOGRAPHY, PHYSICAL Pub Date : 2019-01-01 DOI: 10.5331/bgr.19a02
Oanh Pham Kim(ファンキムオアン), Kazushi NORO(野呂和嗣), Yoshie NABESHIMA(鍋島愛絵), Tatsuya TANIGUCHI(谷口達也), Yusuke FUJII(藤井佑介), Miho ARAI(荒井美穂), Toshimitsu SAKURAI(櫻井俊光), Kenji KAWAMURA(川村賢二), Hideaki MOTOYAMA(本山秀明), Hien To Thi(トティヒエン), Norimichi TAKENAKA(竹中規訓)
The polycyclic aromatic hydrocarbons (PAHs) in snow were measured in eastern Dronning Maud Land, East Antarctica. The sources of these PAHs were the snow mobiles and diesel electric generators used for scientific research from 29th December 2015 to 4th February 2016. Most of the measured PAH concentrations were low before the research campaign (lower than the detection limit or quantification limit), then increased due to the research activities, and finally decreased to a low level (lower than the quantification limit), probably because of strong blizzard winds. In addition, photolysis of the PAHs in the polluted snow samples under Antarctic conditions was investigated. The snow samples polluted by diesel electric generators were collected before and after irradiation under Antarctic sunlight (11 days); however, a decrease in PAH concentration was not observed. It was concluded that photolysis was not the main sink of the PAHs in Antarctic snow, but the occurrence of blizzard/drifting snow decreased the PAH concentration. In this paper, the preservation method for the snow samples to measure the PAHs correctly was also evaluated. To stabilize the PAHs in snow, hexane had to be added to the snow sample. Even if the snow sample was kept in a freezer, PAHs evaporated without hexane.
对南极洲东部Dronning Maud地积雪中的多环芳烃(PAHs)进行了测定。这些多环芳烃的来源是2015年12月29日至2016年2月4日用于科学研究的雪地摩托和柴油发电机。大部分测得的多环芳烃浓度在研究活动前较低(低于检测限或定量限),然后由于研究活动而升高,最后下降到较低水平(低于定量限),这可能与强暴雪风有关。此外,研究了南极条件下污染雪样中多环芳烃的光解作用。采集南极阳光照射前后(11 d)柴油发电机污染的雪样;然而,多环芳烃浓度没有下降。结果表明,光解作用不是南极积雪中多环芳烃的主要吸收途径,但暴雪/飘雪的发生降低了多环芳烃的浓度。本文还对雪样中多环芳烃的保存方法进行了评价。为了稳定雪中的多环芳烃,必须在雪样品中添加己烷。即使雪样本被保存在冰箱里,多环芳烃也会在没有己烷的情况下蒸发。
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引用次数: 5
Twenty-year operation of the Cryospheric Environment Simulator 冰冻圈环境模拟器运行二十年
IF 1 Q4 GEOGRAPHY, PHYSICAL Pub Date : 2019-01-01 DOI: 10.5331/bgr.16sr01
O. Abe, K. Kosugi
The operational results for the Cryospheric Environment Simulator (CES) of the National Research Institute for Earth Science and Disaster Resilience (NIED) compiled over a 20-year period from October 1997 to March 2017, during which a total of 598 projects were conducted, are reported herein. These projects were instigated by four types of institutions: official institutes, universities, companies, and the NIED itself. In terms of international cooperative use, 12 institutes or universities from 9 countries have conducted various investigations using the CES. The present specifications, performances, and operations of CES are described herein; some of the scientific results and future considerations are also presented.
本文报告了1997年10月至2017年3月,中国国家地球科学与灾害恢复研究所冰冻圈环境模拟器(CES) 20年间共开展598个项目的运行结果。这些项目是由四种类型的机构发起的:官方机构、大学、公司和NIED本身。在国际合作使用方面,来自9个国家的12个研究所或大学利用CES进行了各种调查。本文描述CES的现有规格、性能和操作;本文还介绍了一些科学结果和未来的考虑。
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引用次数: 4
Viabilities of long-term cryopreserved CHO-TRET1 cells with trehalose and DMSO 海藻糖和DMSO对CHO-TRET1细胞长期低温保存的影响
IF 1 Q4 GEOGRAPHY, PHYSICAL Pub Date : 2019-01-01 DOI: 10.5331/bgr.18a03
T. Uchida, Maho Furukawa, T. Kikawada, K. Yamazaki, K. Gohara
Trehalose acts as a non-toxic cryoprotectant agent (CPA) to CHO-TRET1 cells, which are CHO-K1 cells that express the trehalose transporter. This study examines the viabilities during long-term storage at 193 K of cryopreserved CHO-TRET1 cells with trehalose, and compares them to the corresponding viabilities with 10 wt%-dimethyl sulfoxide (DMSO). The cryopreserved samples are selected to have a 1-week storage viability exceeding 0.7. Results show that this high viability with trehalose is maintained at the same level for about one year of storage, and gradually decrease to about 0.55 ± 0.16 after about 2.5 years. Comparison of this result with the corresponding case with DMSO suggests that the cryoprotective ability of trehalose is slightly weaker than that of DMSO at this temperature. We discuss the effect of recrystallization of ice, and also the biological damaging, on the viabilities. Then we concluded that the cell membrane slowly degrades at 193 K, with the rate being a little faster with trehalose than with DMSO.
海藻糖作为一种无毒的冷冻保护剂(CPA)作用于CHO-TRET1细胞,CHO-TRET1细胞是表达海藻糖转运体的CHO-K1细胞。本研究检测了含有海藻糖的CHO-TRET1细胞在193 K低温保存期间的存活率,并将其与含有10% -二甲基亚砜(DMSO)的细胞存活率进行了比较。选择冷冻保存的样品,使其1周的储存活力超过0.7。结果表明,海藻糖在贮藏1年左右可保持较高的生存力,贮藏2.5年左右逐渐降至0.55±0.16左右。将这一结果与DMSO的相应情况进行比较,可以看出海藻糖在该温度下的低温保护能力略弱于DMSO。我们讨论了冰的再结晶和生物损伤对生存力的影响。然后我们得出细胞膜在193 K下缓慢降解的结论,海藻糖比DMSO的降解速度稍快。
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引用次数: 2
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Bulletin of glaciological research
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