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A look at polyploidy and plant breeding 多倍体与植物育种
Pub Date : 2022-11-28 DOI: 10.29328/journal.jpsp.1001092
Iannicelli Jesica, Escandón Alejandro S
Polyploidization is a process that generates genetic variability and therefore one of the engines of biological evolution. Since polyploidization produces important changes in the phenotype, mainly an increase in the size of the organs (i.e.: flowers and fruits), it is also a very important and powerful tool for plant improvement. Despite its intense use in breeding programs for various species, very little is known so far about the nature of this phenomenon. This work presents a brief review of the results obtained by the use of this tool in plant breeding and also raises some reflections on its mechanism of action.
多倍体化是一个产生遗传变异的过程,因此是生物进化的引擎之一。由于多倍体化在表型上产生重要的变化,主要是器官(即花和果实)的大小增加,因此它也是植物改良的一个非常重要和有力的工具。尽管它在各种物种的繁殖计划中被大量使用,但迄今为止对这种现象的本质知之甚少。本文简要介绍了利用该工具在植物育种中取得的成果,并对其作用机制提出了一些思考。
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引用次数: 0
The Indole acetic acid in bacteria, fungi and plants 细菌、真菌和植物中的吲哚乙酸
Pub Date : 2022-11-17 DOI: 10.29328/journal.jpsp.1001091
J. Hernández-Mendoza
OPEN ACCESS Indole acetic acid (AIA) is the most important plant growth hormone since it intervenes in the mechanisms of cell growth and differentiation and is produced mostly in the meristematic zones of the plant for apical dominance or root growth. AIA is also produced in root nodules and plant galls. In the latter cases, its presence is associated with endophytic bacteria. Although it is a plant growth hormone, it is also produced by bacteria such as Azospirillum brasilense and Bradyrhizobium japonicum. These species live in the soil (rhizosphere) or can colonize the roots (endophytic) of their hosts. From these bacteria, there are also isolates that are nitrogen ixers, which in addition to providing growth factors to plants, contribute by releasing nitrogenous molecules that improve plant nutrition.
吲哚乙酸(吲哚乙酸,AIA)是一种重要的植物生长激素,它主要在植物的分生组织区产生,用于植物的顶端优势或根的生长,干预细胞生长和分化机制。AIA也在根瘤和植物瘿中产生。在后一种情况下,它的存在与内生细菌有关。虽然它是一种植物生长激素,但它也由巴西氮螺旋菌和日本慢生根瘤菌等细菌产生。这些物种生活在土壤(根际)或可以定殖的根(内生)的寄主。从这些细菌中,也分离出氮混合菌,它们除了为植物提供生长因子外,还通过释放氮分子来改善植物营养。
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引用次数: 0
Post-harvest assessment of infectious fruit rot on selected fruits in Lafia, Nasarawa State Nigeria 尼日利亚纳萨拉瓦州拉菲亚选定水果感染性腐病的收获后评估
Pub Date : 2022-11-15 DOI: 10.29328/journal.jpsp.1001090
Kwon Eh, Terna Tp, Goler Ee, O. G.
The post-harvest health and microbial safety of plant products and foods continue to be a global concern to farmers, consumers, regulatory agencies and food industries. A study was carried out to evaluate the pathogenicity of fungi associated with post-harvest rot of oranges, watermelons and bananas in Lafia, Nasarawa State, Nigeria. Healthy fruits inoculated with fungal spores obtained from rotted fruit tissues were incubated at ambient temperature conditions and observed daily for the appearance and development of tissue rot. Oranges and Watermelons had the highest number of fungal isolates (3) compared to banana (2). Fungi belonging to the genus Curvularia were the most isolated (37.50%), followed by both Aspergillus and Colletotrichum (25.00% respectively) and lastly Alternaria (12.50%). The highest tissue rot diameter of sweet orange (2.40 cm) was induced by Alternaria sp. followed by Curvularia geniculate (1.40 cm) and lastly Colletotrichum sp. (1.28 cm). The highest rot of banana fruit tissues was produced by A. niger (3.90 cm), followed by Curvularia geniculate (3.40 cm). Aspergillus sp. produced the highest tissue rot diameter on watermelon fruits (1.93 cm), followed by Colletotrichum sp. (1.30 cm) and lastly Curvularia geniculate (1.20 cm). Differences in the susceptibilities of different fruits to rot by fungal pathogens were significant (p ≤ 0.05). There is need for improved handling of fruits after harvest to prevent losses due to bacterial and fungal rots in the study area.
植物产品和食品的收获后健康和微生物安全仍然是全球农民、消费者、监管机构和食品行业关注的问题。在尼日利亚纳萨拉瓦州拉菲亚开展了一项研究,以评估与橙子、西瓜和香蕉收获后腐烂有关的真菌的致病性。用从腐烂果实组织中获得的真菌孢子接种健康果实,在室温条件下培养,每天观察组织腐烂的外观和发育情况。与香蕉相比,橙子和西瓜的真菌分离数量最多(3),而香蕉(2)。曲霉属真菌分离数量最多(37.50%),其次是曲霉属真菌和炭疽菌(25.00%),最后是Alternaria真菌(12.50%)。甜橙的组织腐病直径以Alternaria sp.诱导最大,为2.40 cm,其次是曲霉(Curvularia geniculate) (1.40 cm),最后是炭疽病菌(Colletotrichum sp.) (1.28 cm)。香蕉果实组织腐烂率最高的是黑曲霉(3.90 cm),其次是膝曲曲霉(3.40 cm)。西瓜果实的组织腐病直径以曲霉(Aspergillus sp.)最大(1.93 cm),其次是炭疽菌(Colletotrichum sp.) (1.30 cm),最后是曲霉(Curvularia geniculate) (1.20 cm)。不同果实对真菌病原菌腐烂的敏感性差异显著(p≤0.05)。有必要改进果实收获后的处理,以防止研究地区因细菌和真菌腐烂而造成的损失。
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引用次数: 2
Flashes of UV-C light are perceived by UVR8, the photoreceptor of UV-B light UV-C光的闪光由UVR8感知,UVR8是UV-B光的感光器
Pub Date : 2022-11-10 DOI: 10.29328/journal.jpsp.1001089
Aarrouf Jawad, Hdech Douae Ben, Diot Alice, Bornard Isabelle, F. Lauri, Urban Laurent
Light is an important regulator of plant morphogenesis and plant-pathogen interactions via specific photoreceptors and signaling pathways. Besides visible light, other electromagnetic radiations may play roles, notably ultraviolet (UV) light. The UV part of the electromagnetic spectrum includes UV-A (315 nm - 400 nm), UV-B (280 nm - 315 nm) and UV-C radiations (200 nm - 280 nm). UV-B and UV-C have been reported to increase plant resistance to plant pathogens after the UV perception and signaling stages. The perception of UV-B radiation is achieved by the dimer protein UVR8 (UV RESISTANCE LOCUS 8). Even though the action spectrum of this photoreceptor overlaps in the UV-C domain, it has never been formally demonstrated that UVR8 could also act as a photoreceptor of UV-C light. We provide here original observations showing that UVR8 can indeed perceive UV-C light provided that the latter is in the form of flashes (1s) and not continuous illuminations (the 60s). Our observations also show that the response of UVR8 to flashes of UV-C light is dose-dependent. They could explain why flashes of UV-C light are more effective for stimulating plant defenses than continuous illuminations for the same amount of energy delivered to plants (J/m2). Eventually, our observations support ongoing trials that aim at using UV-C light as an environmental-friendly plant resistance inducer in field conditions.
光是植物形态发生和植物病原体通过特定的光感受器和信号通路相互作用的重要调节因子。除了可见光,其他电磁辐射也可能起作用,特别是紫外线。电磁光谱的UV部分包括UV-A(315nm-400nm)、UV-B(280nm-315nm)和UV-C辐射(200nm-280nm)。据报道,UV-B和UV-C在紫外线感知和信号传导阶段后增加了植物对植物病原体的抗性。对UV-B辐射的感知是通过二聚体蛋白UVR8(UV抗性位点8)实现的。尽管这种感光体的作用光谱在UV-C结构域中重叠,但从未正式证明UVR8也可以作为UV-C光的感光体。我们在这里提供了原始观察结果,表明UVR8确实可以感知UV-C光,前提是后者是闪光(1s)而不是连续照明(60s)的形式。我们的观察结果还表明,UVR8对UV-C光闪光的反应是剂量依赖性的。他们可以解释为什么在向植物输送相同能量(J/m2)的情况下,UV-C光的闪光比连续照明更有效地刺激植物防御。最终,我们的观察结果支持了正在进行的试验,该试验旨在在田间条件下使用UV-C光作为环境友好的植物抗性诱导剂。
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引用次数: 0
Assessment of indigenous methods of shea butter processing among rural women in Borgu Local Government Area of Niger State, Nigeria 尼日利亚尼日尔州博古地方政府区农村妇女乳木果油加工本土方法的评估
Pub Date : 2022-11-04 DOI: 10.29328/journal.jpsp.1001088
S. Ibrahim, M. Musa
Indigenous food processing and preservation methods are on the verge of collapse, yet they proved promising and sustainable. The study assessed the indigenous methods of shea butter processing among rural women in the Borgu Local Government Area of Niger State, Nigeria. Specifically, it described the socioeconomic characteristics of respondents, examined the shea butter processing techniques used and identified the information sources of shea butter processors in the study area. A multistage sampling technique was used to select 100 respondents. Descriptive (such as frequency count, percentage, charts and tables) and inferential statistics (such as Pearson correlation and chi-square) were used to analyze the data. Findings showed the mean age of respondents was 45.61 ± 11.82, with mean years of experience of 20.39 ± 12.96, the majority (85%) were married and the major sources of information on indigenous shea butter processing came from family members and friends. At p ≤ 0.01 there was a significant association between respondents’ usage of indigenous methods and their marital status (χ2 = 84.24; p ≤ 0.01), membership in cooperative society (χ2 = 40.43; p ≤ 0.01), and community membership (χ2 = 53.21; p ≤ 0.01). However, there was a significant relationship between respondents’ usage of indigenous methods and household size (b = 0.290; p ≤ 0.05), quantity produced (b = 0.616; p ≤ 0.10) and annual income (b = -0.765; p ≤ 0.05). It was concluded that indigenous methods of processing shea butter are widespread among respondents; knowledge is acquired through family and friends. Among others, the study recommends that extension agents be posted to rural areas to educate rural women and build on their indigenous knowledge of processing shea butter to introduce high-quality butter.
土著食品加工和保存方法正处于崩溃的边缘,但它们被证明是有前途和可持续的。该研究评估了尼日利亚尼日尔州博尔古地方政府地区农村妇女加工乳木果油的土著方法。具体来说,它描述了受访者的社会经济特征,检查了所使用的乳木果油加工技术,并确定了研究地区乳木果油加工商的信息来源。采用多阶段抽样技术,选取100名受访者。使用描述性统计(如频率计数、百分比、图表和表格)和推断性统计(如Pearson相关和卡方)来分析数据。调查结果显示,受访者的平均年龄为45.61±11.82岁,平均工作经验为20.39±12.96年,大多数(85%)已婚,当地乳木果油加工信息主要来自家人和朋友。在p≤0.01时,调查对象使用本地方法与婚姻状况有显著相关(χ2 = 84.24;P≤0.01),合作社成员数(χ2 = 40.43;P≤0.01)、社区成员(χ2 = 53.21;P≤0.01)。然而,调查对象对本地方法的使用与家庭规模之间存在显著关系(b = 0.290;P≤0.05),产量(b = 0.616;P≤0.10)、年收入(b = -0.765;P≤0.05)。结论是,当地加工乳木果油的方法在答复者中普遍存在;知识是通过家人和朋友获得的。除其他外,该研究建议向农村地区派遣推广人员,对农村妇女进行教育,并利用她们加工乳木果油的本土知识,向她们介绍高质量的乳木果油。
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引用次数: 0
Characterization of senegalese races of Xanthomonas oryzae PV. oryzae to identify resistance genes to use 稻黄单胞菌塞内加尔小种的特性研究。鉴定稻瘟病抗性基因
Pub Date : 2022-10-19 DOI: 10.29328/journal.jpsp.1001087
H. Tall, C. Tékété, A. Comte, K. Noba, M. Hutin, B. Szurek, V. Verdier, S. Cunnac
Bacterial blight (BB), is a disease caused by Xanthomonas oryzae PV. oryzae (Xoo), was first reported in Senegal by Trinh in 1980. BB represents a severe threat to rice cultivation in West Africa. Characterizing the pathotypic diversity of bacterial populations is a key to the management of pathogen-resistant varieties. Pathogenicity tests show that all strains are virulent on the susceptible rice variety Azucena, and interact differentially with twelve near-isogenic rice lines, each carrying a single resistance gene. On this rice panel, six races were identified, two of which were previously reported in Mali (A3) and Burkina Faso (A1). Four races (S2, S4, S5, and S6) are described for the first time in Africa. Races A1, isolated in Ndiaye and Ndioum areas is the most prevalent in Senegal. The Xa1 gene controls 100% of the isolates tested and xa5 controls all isolates except S4 strains. The geographical distribution of Xoo races is contrasted. Four races are detected in the North and two in the South East of the country. Race S4 can be a major risk to rice cultivation because strains from this race are the most virulent and can only be controlled by Xa1. To identify local sources of resistance, we screened Xoo strains representative of the various races on twenty-three rice varieties grown by farmers in Senegal. Four rice varieties namely Sahel210, Sangangbye, Dansna2, and Sahel305 effectively control all the isolates tested. Our characterization of the first collection of Senegalese Xoo strains provided insight into the races present in the country and identified sources of resistance in local rice varieties. This information will help design effective breeding programs for resistance to bacterial leaf blight in Senegal.
细菌性枯萎病(BB)是一种由米黄单胞菌引起的疾病。oryzae (Xoo)于1980年由Trinh在塞内加尔首次报道。BB对西非的水稻种植构成严重威胁。细菌种群的病型多样性特征是抗病品种管理的关键。致病性试验表明,所有菌株对易感水稻品种Azucena都有毒力,并且与12个近等基因水稻品系有不同的相互作用,每个品系携带一个抗性基因。在这个水稻面板上,确定了六个品种,其中两个以前在马里(A3)和布基纳法索(A1)报告过。4个种(S2, S4, S5和S6)首次在非洲被描述。在Ndiaye和Ndioum地区孤立的A1种族在塞内加尔最为普遍。Xa1基因控制100%的分离株,xa5基因控制除S4菌株外的所有分离株。对比了Xoo个种族的地理分布。在北部发现了四个种族,在该国东南部发现了两个种族。小种S4可能是水稻种植的主要风险,因为来自该小种的毒株毒性最强,只能由Xa1控制。为了确定当地的抗性来源,我们在塞内加尔农民种植的23个水稻品种上筛选了代表不同种族的Xoo个菌株。4个水稻品种Sahel210、Sangangbye、Dansna2和Sahel305均能有效控制所有被试菌株。我们对第一批塞内加尔Xoo菌株的特征描述提供了对该国存在的种族的深入了解,并确定了当地水稻品种的抗性来源。这些信息将有助于在塞内加尔设计有效的抗细菌性叶枯病育种方案。
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引用次数: 0
The ongoing search for sustainable agriculture 对可持续农业的持续探索
Pub Date : 2022-10-07 DOI: 10.29328/journal.jpsp.1001086
Friedrich Theodor
OPEN ACCESS Agricultural land use has left its traces on the planet since sedentary agriculture started. Most of the ancient cradles of mankind and agriculture, such as the area between the rivers Euphrates and Tigris or the middle east are degraded and deserts today. Yet, it was only after the devastating dust bowls in prairies of the USA and Canada that people started rethinking agricultural practices, in that case the soil tillage. Conservation tillage as erosion reducing practice was developed [1]. But environmental degradation continued. Particularly after World War II, but even more so with the success of the green revolution, agriculture became a major factor in environmental degradation as it tried to replace natural processes with technology [2]. Severe environmental problems from pesticide pollution, eutrophication of waters with nitrates and phosphates, declining biodiversity are only some problems which continue worldwide without real improvement. At the same time yields started to plateau despite increasing input use, in some cases yields even declined. Organic agriculture is seen in this context as a solution as it uses no synthetic inputs. Yet, it has not proven a practical solution for widespread adoption, and it has still a major problem with soil erosion and soil biodiversity as well as the adaptation to climate change.
自从定居农业开始以来,农业用地在地球上留下了痕迹。大多数古老的人类和农业发源地,如幼发拉底河和底格里斯河之间的地区或中东地区,今天已经退化和沙漠化。然而,只有在美国和加拿大大草原遭受毁灭性的沙尘暴之后,人们才开始重新思考农业实践,在这种情况下就是土壤耕作。开发了保护性耕作作为减少侵蚀的做法[1]。但环境恶化仍在继续。特别是在第二次世界大战之后,但随着绿色革命的成功,农业成为环境退化的主要因素,因为它试图用技术取代自然过程[2]。农药污染、硝酸盐和磷酸盐造成的水体富营养化、生物多样性下降等严重的环境问题只是世界范围内持续存在的一些问题,没有得到真正的改善。与此同时,尽管投入使用增加,但产量开始趋于平稳,在某些情况下产量甚至下降。在这种情况下,有机农业被视为一种解决方案,因为它不使用合成投入。然而,它并没有被证明是一个广泛采用的实际解决方案,它仍然是土壤侵蚀和土壤生物多样性以及适应气候变化的一个主要问题。
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引用次数: 2
Micropropagation and cytological studies of Aole vera Linn 芦荟的微繁殖及细胞学研究
Pub Date : 2022-09-30 DOI: 10.29328/journal.jpsp.1001085
M. Sweety, Biswas Animesh, Rahman Mohammad Mahbubur
Aloe vera Linn. is an essential medicinal plant. In this present research work, a protocol of in vitro regeneration and karyomorphological analysis of Aloe vera was developed using different concentrations and compositions of media. Shoot apices of field-grown plants were used as explant and aseptically cultured on Murashige and Skoog (MS) medium fortified with different concentrations and combinations of auxins (IAA and NAA) and cytokinins (BAP and Kn). The highest number of multiple shoot buds (4.36 ± 0.07) was obtained from MS + 2.0 mg/l BAP + 1.0 mg/l IAA and induced shoot buds underwent rapid elongation (4.24 ± 0.06 cm) on the same medium composition. Half strength MS media with 2.0 mg/l IBA was suitable for induction and proliferation (6.31 ± 0.05) of roots and 95% of plantlets were acclimatized to field conditions successfully. Somatic chromosome numbers of mother and in vitro grown plants were confirmed to be 2n = 14. Chromosome length ranged from 4.28 - 13.74 µm in the naturally grown plants and 4.46 - 14.1 µm for in vitro grown plants. The total form percent (TF%) of mother and in vitro grown plants was 41.69% and 42.23%, respectively. The karyotype formula of in vivo grown plants was 2n = 14 = 4Lsm + 6Mm + 4Sm, whereas that of the micropropagated plants was 2n = 14 = 4Lsm + 4Mm + 6Sm. The frequency of the chromosome having arm more than 2:1 was 0.08 for mother plants and 0.15 for in vitro grown plants. Therefore, the karyotype of both plants falls into the 2B symmetrical type based on Stebbins classification (1971).
芦荟。是一种重要的药用植物。在本研究中,研究了芦荟在不同浓度和不同成分培养基下的体外再生和核形态分析方案。以大田植株的茎尖为外植体,在添加不同浓度和组合的生长素(IAA和NAA)和细胞分裂素(BAP和Kn)的Murashige和Skoog (MS)培养基上无菌培养。MS + 2.0 mg/l BAP + 1.0 mg/l IAA的诱导芽数最多,为4.36±0.07,在相同培养基组成下,诱导芽迅速伸长(4.24±0.06 cm)。添加2.0 mg/l IBA的半强度MS培养基可诱导和增殖(6.31±0.05)根,95%的植株成功适应大田条件。母株和离体植株的体细胞染色体数均为2n = 14。天然植株的染色体长度为4.28 ~ 13.74µm,离体植株的染色体长度为4.46 ~ 14.1µm。母株和离体植株的总成形率分别为41.69%和42.23%。活体植株核型公式为2n = 14 = 4Lsm + 6Mm + 4Sm,微繁植株核型公式为2n = 14 = 4Lsm + 4Mm + 6Sm。染色体臂大于2:1的频率,母株为0.08,离体植株为0.15。因此,根据Stebbins(1971)的分类,这两种植物的核型都属于2B对称型。
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引用次数: 0
Photocatalytic degradation of organic pollutants in the presence of selected transition metal nanoparticles: review 在选定的过渡金属纳米颗粒存在下光催化降解有机污染物:综述
Pub Date : 2022-09-29 DOI: 10.29328/journal.jpsp.1001084
Mekonnen Tigabu Bekele
Photocatalysis has attracted a lot of attention in recent years due to its potential in solving energy and environmental issues. Efficient light absorption and charge separation are two of the key factors for the exploration of high-performance photocatalytic systems, which are generally difficult to obtain from a single photocatalyst. The combination of various materials to form heterojunctions provides an effective way to better harvest solar energy and facilitate charge separation and transfer, thus enhancing photocatalytic activity and stability. This review concisely summarizes the recent development of visible light responsive heterojunctions, including the preparation and performance of semiconductor/semiconductor junctions and semiconductor/metal junctions and their mechanism for enhancing light harvesting and charge separation/transfer. In this regard, this review presents some unitary, binary and ternary CeO2 photocatalysts used for the degradation of organic pollutants. We expect this review to provide the type of guidelines for readers to gain a clear picture of nanotechnology and the fabrication and application of different types of heterostructured photocatalysts.
近年来,光催化因其在解决能源和环境问题方面的潜力而引起了人们的广泛关注。高效的光吸收和电荷分离是探索高性能光催化系统的两个关键因素,通常很难从单个光催化剂中获得。将各种材料组合形成异质结提供了一种更好地获取太阳能并促进电荷分离和转移的有效方法,从而增强了光催化活性和稳定性。这篇综述简要总结了可见光响应异质结的最新发展,包括半导体/半导体结和半导体/金属结的制备和性能,以及它们增强光捕获和电荷分离/转移的机制。在这方面,本文介绍了一些用于降解有机污染物的一元、二元和三元CeO2光催化剂。我们希望这篇综述能为读者提供一种指南,让他们清楚地了解纳米技术以及不同类型异质结构光催化剂的制造和应用。
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引用次数: 1
An overview of the developments of nanotechnology and heterogeneous photocatalysis in the presence of metal nanoparticles 综述了纳米技术和金属纳米颗粒存在下的非均相光催化的发展
Pub Date : 2022-09-20 DOI: 10.29328/journal.jpsp.1001083
Mekonnen Tigabu Bekele
In general, nanotechnology can be understood as a technology of design, fabrication and applications of nanostructures and nanomaterials, as well as a fundamental understanding of the physical properties and phenomena of nanomaterials and nanostructures. In recent years the development of industries like textile, leather, paint, food, plastics, and cosmetics is enlarged and these industries are connected with the discarding of a vast number of organic pollutants which are harmful to microbes, aquatic systems, and human health by influencing the different parameters. So the fabrication of those nanomaterials (coupled or doped) to form heterojunctions provides an effective way to better harvest solar energy and facilitate charge separation and transfer, thus enhancing the photocatalytic activity and stability. We expect this review to provide a guideline for readers to gain a clear picture of the fabrication and application of different types of heterostructured photocatalysts. In this review, starting from the photocatalytic reaction mechanism and the preparation of the photocatalyst, we review the classification of current photocatalysts, preparation methods, a factor that affects photocatalytic reaction, characterization of photocatalysts, and the methods for improving photocatalytic performance. This review also aims to provide basic and comprehensive information on the industrialization of photocatalysis technology.
总的来说,纳米技术可以理解为纳米结构和纳米材料的设计、制造和应用技术,以及对纳米材料和纳米结构的物理性质和现象的基本理解。近年来,纺织、皮革、油漆、食品、塑料和化妆品等行业的发展不断扩大,这些行业与大量有机污染物的排放有关,这些污染物通过影响不同的参数对微生物、水生系统和人体健康有害。因此,制造这些纳米材料(耦合或掺杂)形成异质结提供了一种有效的方法,可以更好地收集太阳能,促进电荷的分离和转移,从而提高光催化活性和稳定性。希望本文能对不同类型异质结构光催化剂的制备和应用有一个清晰的认识。本文从光催化反应机理和光催化剂的制备入手,综述了目前光催化剂的分类、制备方法、影响光催化反应的因素、光催化剂的表征以及提高光催化性能的方法。为光催化技术的产业化提供基础和全面的信息。
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引用次数: 1
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