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Carotenoid Content and Profile of Some Commercially Available Eggs and In Vitro Bioaccessibility of Lutein and Zeaxanthin from Organic Egg Yolks 部分市售鸡蛋类胡萝卜素含量和特征及有机蛋黄叶黄素和玉米黄质的体外生物可及性
Pub Date : 2021-05-16 DOI: 10.15835/BUASVMCN-FST:2021.0004
Mariana Alexandra Piţurlea, A. Pintea, Florina Copaciu, Elena Gherasim, Cristina Tudor
The present study investigated the carotenoid content and composition of several commercial eggs produced in different housing systems and the amount of macular xanthophylls that becomes available for absorption by the intestinal cells (bio-accessible) after the ingestion of boiled organic egg yolks. The highest total carotenoid content was observed in the case of organic egg yolks (7.02 mg/100 g). Carotenoid concentration varied not only between egg yolks obtained through different housing methods (free-range, barn and battery-cage), but also between egg yolks from the same housing method obtained by different producers. Except for organic egg yolks that had lutein and zeaxanthin as the major carotenoids, canthaxanthin was observed in all the investigated egg yolks to a different extent. Both lutein and zeaxanthin displayed a high bioaccessibility from boiled organic egg yolks, 86% and 91%, respectively).
本研究调查了在不同饲养系统中生产的几种商品鸡蛋的类胡萝卜素含量和组成,以及食用煮熟的有机蛋黄后可被肠道细胞吸收的黄斑叶黄素的数量(生物可及性)。有机蛋黄的类胡萝卜素总含量最高,为7.02 mg/100 g。类胡萝卜素的含量不仅在不同饲养方式(散养、圈养和网箱)的蛋黄之间存在差异,而且在不同生产厂家生产的相同饲养方式的蛋黄之间也存在差异。除有机蛋黄中叶黄素和玉米黄质为主要类胡萝卜素外,其余蛋黄中均存在不同程度的角黄素。叶黄素和玉米黄质在煮熟的有机蛋黄中具有较高的生物可及性(分别为86%和91%)。
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引用次数: 0
Effect of Phytase Addition to Buckwheat Wort on the Selected Fermentable Sugars, Polypeptide Profile and Nitrogen Content from Free Aminoacids 添加植酸酶对荞麦汁可发酵糖、多肽谱和游离氨基酸氮含量的影响
Pub Date : 2021-05-16 DOI: 10.15835/BUASVMCN-FST:2020.0053
R. Duliński, M. Zdaniewicz, A. Pater
Relatively high levels of phytates in buckwheat malt and the low activity of endogenous phytases that limit the effective use of substrates for fermentation and yeast metabolism (starch, proteins, minerals) are an argument for using phytases in beer production technology. Two mash-in programs were applied: (1) the Congress program, typical for basic raw materials, (2) a program with temperature optimized for phytase activity. Commercial preparations of 3-phytase (Finase P) and 6-phytase (Ronozyme) were used in the study. Monitored levels of selected fermentable sugars indicates a statistically significant effect of phytase addition on the glucose content in both mash-in programs used. The SEC-HPLC chromatography allowed to select a key polypeptide with an estimated molecular weight of 40 kDa, whose relative peak area decreases as a result of the applied mashincrease treatment with phosphorolytic enzymes, although this relation was not statistically confirmed in the analysis of free amino acids content. The analyses carried out also indicate that apart from the target molecules, namely phytate and inositol, the use of phytases in the process of buckwheat wort preparation slightly changes the profile of fermentable sugars and causes significant changes in the polypeptide profile of the final mash.
荞麦麦芽中相对较高的植酸水平和内源性植酸酶的低活性限制了发酵和酵母代谢底物(淀粉,蛋白质,矿物质)的有效利用,这是在啤酒生产技术中使用植酸酶的一个理由。采用了两种混包程序:(1)Congress程序,典型的基本原料;(2)温度优化程序,以提高植酸酶的活性。采用市售的3-植酸酶(Finase P)和6-植酸酶(Ronozyme)。选定的可发酵糖的监测水平表明,在使用的两种mashin程序中,添加植酸酶对葡萄糖含量有统计学上显著的影响。SEC-HPLC色谱允许选择一个估计分子量为40 kDa的关键多肽,其相对峰面积由于应用了磷酸化酶的mashincrease处理而减少,尽管这种关系在分析游离氨基酸含量时没有统计学上的证实。分析还表明,除了目标分子,即植酸盐和肌醇外,在荞麦麦芽汁制备过程中使用植酸酶会轻微改变可发酵糖的特征,并导致最终醪的多肽特征发生重大变化。
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引用次数: 3
Conservation of Genetic Diversity of Tomatoes (Lycopersicon esculentum Mill.) 番茄(Lycopersicon esculentum Mill.)遗传多样性保护
Pub Date : 2021-05-14 DOI: 10.15835/BUASVMCN-AGR:2021.0001
C. Albu, A. Maxim, Raluca-Maria Pârlici
Among the main problems encountered with crop plants, the most important one is represented by genetic erosion. At world level this issue has been debated within the Convention on Biodiversity adopted in UN Conference of Rio de Janeiro and The International Convention regarding Genetic Resources of Plants for Alimentation and Agriculture. In Europe, the regulations in this field are made by the European Directive 98/95 EEC. Across time tomatoes have suffered a heightened process of genetic diversity, phenomenon caused by industrialized farming which is based on the use of very uniform varieties with high productivity. The extension of green agriculture, has led to the use of landraces, because they respond best to the traditional farming systems. In Western Europe organizations of peasants had been founded and, they are involved in different activities aiming at conservation of traditional forms of agriculture and the use of old varieties. In Romania the institutions with continuous preoccupations in the field of vegetal genetic conservation, especially landraces, are the Gene Bank from Suceava and UASMV Cluj-Napoca. By using conservation methods (in situ and ex situ), the genetic erosion surely phenomenon of vegetal genetic resources, implicitly tomatoes, is reduced, the future generations will benefit from valuable genetic resources.
在农作物所遇到的主要问题中,最重要的是遗传侵蚀。在世界一级,这一问题已在联合国里约热内卢会议通过的《生物多样性公约》和《营养和农业植物遗传资源国际公约》中进行了辩论。在欧洲,该领域的法规是由欧洲指令98/95 EEC制定的。随着时间的推移,西红柿经历了遗传多样性提高的过程,这种现象是基于使用非常统一的高产品种的工业化农业造成的。绿色农业的扩展导致了对地方品种的使用,因为它们对传统农业系统的反应最好。在西欧建立了农民组织,他们参与旨在保护传统农业形式和利用旧品种的各种活动。在罗马尼亚,一直致力于植物遗传保护领域,特别是地方植物遗传保护的机构是来自苏切瓦的基因库和克卢日纳波卡地区植物遗传保护协会。通过采用就地保护和移地保护的方法,减少了番茄等植物遗传资源的遗传侵蚀现象,使后代从宝贵的遗传资源中受益。
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引用次数: 0
Genetic Diversity of Elaeagnus angustifolia L. (Elaeagnaceae) Populations in İzmir (Turkey) 土耳其İzmir地区Elaeagnus angustifolia L. (Elaeagnus aceae)居群遗传多样性
Pub Date : 2021-05-14 DOI: 10.15835/BUASVMCN-AGR:2020.0048
Erengül Sofyalioğlu, E. Sevindik, H. Uysal
This study was performed out genetic diversity of some Elaeagnus angustifolia L. populations growing in İzmir province by using ISSR markers. In the study, PCR was performed using 15 ISSR primers. PCR products were run in agarose gel and visualized under UV light. Amplified products were scored as follows. A total of 46 bands were produced from 15 ISSR primers, of which 27 were polymorphic. The proportion of polymorphic bands was evaluated as approximately 58.7%. Genetic distances between phylogenetic trees and genotypes were calculated using the PAUP program. The phylogenetic tree consists of two large clades. The longest distance between populations was between Gümüldür-Özdere and Çeşme-Alaçatı population with a value of 0.50, while the closest distance was between Çeşme-Ayayorgi and Konak-Hatay populations with a value of 0.06. The results show that ISSR markers are useful tools for determining genetic relationships between E. angustifolia populations.
利用ISSR标记分析了İzmir省部分榆群的遗传多样性。本研究使用15条ISSR引物进行PCR。PCR产物在琼脂糖凝胶中运行,并在紫外线下观察。扩增产物评分如下。从15条ISSR引物中共得到46条条带,其中27条为多态性条带。多态性条带的比例约为58.7%。使用PAUP程序计算系统发育树和基因型之间的遗传距离。系统发育树由两个大支系组成。居群间距离以g ldld r-Özdere与Çeşme-Alaçatı居群间距离最远,为0.50,居群间距离以Çeşme-Ayayorgi与Konak-Hatay居群间距离最近,为0.06。结果表明,ISSR标记可作为确定刺叶居群间亲缘关系的有效工具。
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引用次数: 1
Frequency of Dark Septate Endophytes Colonization in Iris germanica L. 德国鸢尾暗隔内生菌定殖频率。
Pub Date : 2021-05-14 DOI: 10.15835/BUASVMCN-AGR:2020.0045
I. Crișan
Dark septate endophytes (DSE) are a group of fungi from phylum Ascomycota that develop inside healthy root tissue of a wide range of plants from diverse habitats. They play a role in nutrient acquisition and survival of their hosts in limiting conditions. Aim of this research was to identify the seasonal occurrence of dark septate endophytes across a cultivar gradient in urban conditions from Cluj-Napoca, Romania. Root samples from six Iris germanica cultivars were collected in spring and autumn. Microscopic assessment was conducted on 2160 root segments. Average DSE frequency in roots of Iris germanica was 14.58%. Analysis of variance revealed that influence exercised by the cultivar was not significant (p=0.37), but the interaction between cultivar and phenophase explained 53.97% of overall variance. DSE were identified in all six cultivars, indicating either to a similar susceptibility of the host genotype or lack of specificity of the fungal endophyte. Unravelling the functional roles of these fungi could contribute to a better understating of plant-fungi interactions in anthropic environments.
暗隔内生菌(Dark separated endophytes, DSE)是子囊菌门的一类真菌,生长在不同生境的多种植物的健康根组织中。它们在有限条件下对宿主的营养获取和生存起着重要作用。本研究的目的是在罗马尼亚克卢日-纳波卡的城市条件下,确定跨栽培梯度的暗隔内生菌的季节性发生。在春季和秋季采集了6个德国鸢尾品种的根样。对2160根进行了显微评价。德国鸢尾根部的平均DSE频率为14.58%。方差分析结果显示,品种间的交互作用不显著(p=0.37),但品种与物候期的交互作用解释了总方差的53.97%。在所有6个品种中都发现了DSE,这表明要么对宿主基因型有相似的易感性,要么对真菌内生菌缺乏特异性。揭示这些真菌的功能角色有助于更好地理解人类环境中植物与真菌的相互作用。
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引用次数: 0
Improvement of the Water Management by Applying the No Tillage System for the Winter Wheat Production 冬小麦免耕制度对水分管理的改进
Pub Date : 2021-05-14 DOI: 10.15835/BUASVMCN-AGR:2020.0040
F. Chețan, C. Chețan
The Transylvanian Plain has a particular aspect, as the hilly areas are predominant and here the soil erosion phenomena through levigation are frequently encountered. The effect of water availability on yields has been thoroughly researched, being one of the main limitative factors of the winter wheat yields. Starting from these considerations, during the agricultural years 2012-2018, a bi-factorial yield test was performed at Agricultural Research and Development Station (ARDS) Turda, for a period of seven years. The study explored the influence of the water status under the influence of two tillage systems and the climatic conditions specific for this area on winter wheat yields. In the field conditions of Turda during experimental interval, the soil tillage system had a rather small impact on the winter wheat yields. In the conventional system (CS) a reserve of accessible water is preserved better during the spring period, this being attributed to a better infiltration into the soil of winter precipitation (snow). In the conservative system (NT), the observed trend suggests that restoration of the ground water reserve is more difficult than in the CS but the loss of water in the CS is just as fast.
特兰西瓦尼亚平原有一个特殊的方面,因为丘陵地区占主导地位,这里经常遇到通过航行造成的土壤侵蚀现象。水分有效性是制约冬小麦产量的主要因素之一,对产量的影响研究较为深入。从这些考虑出发,在2012-2018农业年,在图尔达农业研究与发展站(ARDS)进行了为期7年的双因子产量试验。探讨了两种耕作制度下的水分状况和该地区特有的气候条件对冬小麦产量的影响。在吐尔达试验期的大田条件下,土壤耕作制度对冬小麦产量的影响较小。在常规系统(CS)中,可利用水的储备在春季保存得更好,这是由于冬季降水(雪)更好地渗入土壤。在保守系统(NT)中,观察到的趋势表明,地下水储量的恢复比在CS中更困难,但CS中的水分损失同样快。
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引用次数: 0
Phytoremediation Potential of Iris spp. 鸢尾属植物修复潜力研究
Pub Date : 2021-05-14 DOI: 10.15835/BUASVMCN-AGR:2020.0046
I. Crișan, R. Vidican, A. Pleșa, T. Mihăiescu
Iris plants are widely cultivated flowering ornamentals, with a long history of traditional use in Eurasia, where this genus is reaching the highest diversity. This paper aims to provide an overview on recent advances related to the phytoremediation potential of plants from the genus Iris, in order to promote the use of these species in phytoremediation programs. According to the relevant literature, eight species from genus Iris present phytoremediation potential (I. dichotoma, I. germanica, I. halophila, I. lactea, I. latifolia, I. pseudacorus, I. sibirica, I. wilsonii). The studies addressed potential of plants to mitigate toxic metals/metalloids (As, Cd, Cr, Cu, Fe, Hg, Mn, Ni, Pb, Zn), excess of nutrients (P, N), pesticides, petroleum hydrocarbons, pharmaceuticals as well as dyes. Most studies focused on wastewater treatment and environments contaminated due to mining activities. Main hindrances in upscaling this green technology remain mitigation of toxicity stress in plants during remediation and the disposal of resulting contaminated biomass. In this sense, use of beneficial microorganisms to alleviate phytotoxicity effects and new valorization possibilities of contaminated Iris spp. biomass have been proposed recently. Designing an entire cycle that includes phytoremediation and sustainable value chains for contaminated biomass could prove feasible and should receive more attention.
鸢尾属植物是广泛种植的开花观赏植物,在欧亚大陆具有悠久的传统使用历史,该属的多样性达到最高。本文就鸢尾属植物修复潜力的研究进展进行综述,以期促进鸢尾属植物在植物修复中的应用。根据相关文献,鸢尾属中8种鸢尾具有植物修复潜力(I. dichotoma、I. germanica、I. halophila、I. lactea、I. latifolia、I. pseudacorus、I. sibirica、I. wilsonii)。这些研究探讨了植物减轻有毒金属/类金属(As, Cd, Cr, Cu, Fe, Hg, Mn, Ni, Pb, Zn),过量营养物质(P, N),农药,石油烃,药物和染料的潜力。大多数研究集中在废水处理和采矿活动污染的环境上。扩大这一绿色技术的主要障碍仍然是在修复过程中减轻植物的毒性应激和处理由此产生的污染生物质。从这个意义上说,利用有益微生物来减轻鸢尾属植物的毒性作用和污染的生物量的新的增值可能性最近被提出。为受污染的生物质设计一个包括植物修复和可持续价值链的整个循环可能是可行的,应该得到更多的关注。
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引用次数: 2
Dissociation of Rhodococcus rhodochrous Population after the Whole Cells Immobilization 全细胞固定化后红红球菌群体的分离
Pub Date : 2021-05-14 DOI: 10.15835/BUASVMCN-AGR:2020.0043
I. Rastimesina, O. Postolachi, V. Josan
Six agricultural organic wastes and three inorganic matrices were selected for rhodococci whole cells immobilization. The degree of immobilization of rhodococci cells varied from 6.20% to 34.30% on organic matrices. A high level of Rhodococcus rhodochrous CNMN-Ac-05 cells immobilization was demonstrated on inorganic matrices, it was from 69.25% to 97.30%. After the contact with support the strain dissociated, forming, in addition to original S type, rough (R) and altercolour smooth (S) types. Immobilization of rhodococci cells on organic supports led to the appearance of phenotypic heterogeneity from 0.34% to 3.26%. On inorganic matrices the variability of rhodococci was 0.88-1.05%.
选择6种农业有机废弃物和3种无机基质对红球菌全细胞进行固定化。红球菌细胞在有机基质上的固定化程度为6.20% ~ 34.30%。红红红球菌CNMN-Ac-05细胞在无机基质上的固定化率为69.25% ~ 97.30%。在与支架接触后,应变解离,形成除原始S型外,粗糙(R)和变色光滑(S)型。将红球菌细胞固定在有机载体上导致表型异质性在0.34% ~ 3.26%之间。在无机基质上,红球菌的变异率为0.88 ~ 1.05%。
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引用次数: 0
Evaluation of the Amount of CO2 in the Fermentation of Gluten Free Dough with Potato and Sauerkraut Juice 马铃薯酸菜汁无麸质面团发酵过程中CO2含量的评价
Pub Date : 2021-05-04 DOI: 10.15835/BUASVMCN-FST:2020.0031
M. Iancu
Potato puree and sauerkraut juice are valuable raw material resources that can improve the ability of the gluten free dough to release gas and CO2. The starchy carbohydrates in the potatoes, modified due to the heat treatment and the vitamins, minerals, salt, acids, cellulose, sugars in the sauerkraut juice are a rich source of elements to increase the amount of gas, thus an improvement in the fermentation of the gluten free dough. This study evaluated the amount of CO2, using a volumetric method and the batch bioreactor to study the fermentation of a "gluten free" dough made of rice flour which is replaced with potato puree in proportion of 10%, 20%, 30%, 50%, 70% and water replaced with sauerkraut juice according to the material balance sheet. The results showed that the highest amount of CO2, of 1670 ml NaCl, was obtained in the sample where 10% of the flour was replaced with potato puree, sauerkraut juice was used and the dough moisture was 60%. Because the reference taken was the dough made from commercially available mixes combined with water, it was observed that the new materials used in this study are a good choice, being natural and healthy for the body, able to produce high volume gluten free products and being a waste recovery variant in the food industry.
马铃薯泥和酸菜汁是提高无麸质面团释放气体和二氧化碳能力的宝贵原料资源。土豆中的淀粉类碳水化合物经过热处理后得到了改善,而酸菜汁中的维生素、矿物质、盐、酸、纤维素和糖是增加气体量的丰富元素来源,从而改善了无麸质面团的发酵。本研究利用容积法和间歇式生物反应器,根据物料平衡表,以10%、20%、30%、50%、70%比例的土豆泥代替米粉,用酸菜汁代替水,对“无麸质”面团的发酵进行了CO2量评价。结果表明,以马铃薯泥代替10%的面粉、用酸菜汁、面团含水量为60%的样品中CO2含量最高,为1670 ml NaCl。由于所采用的参考材料是由市购的混合物与水混合制成的面团,因此观察到本研究中使用的新材料是一个很好的选择,对身体有益,天然且健康,能够生产大量无麸质产品,并且是食品工业中废物回收的变体。
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引用次数: 0
Evaluating Plant Biodiversity in Natural and Semi‑Natural Areas with the Help of Aerial Drones 利用无人机评估自然和半自然地区的植物生物多样性
Pub Date : 2020-11-20 DOI: 10.15835/buasvmcn-agr:2020.0007
Dragomir D. Sângeorzan, I. Rotar
In the era of abundant information, wisdom has become a scarce resource. Exploration and measurement of biodiversity yield copious amounts of information fragmented into numerous categories and types, following the pattern of life itself. We aim to review the potential of charting the plant diversity of woodlands, grasslands, marshlands, and other natural and semi-natural areas relevant to conservation by using low-altitude, low-cost, Earth Observation technology, such as small aerial drones, that captures images which can be mapped and analyzed. As this technology is new and quickly evolving, we limited the search to published research between 2012-2019. We have found applications for forestry, agro-forestry, horticulture, grasslands, ecological restoration, and other areas. In terms of technological progress, new hardware components and cameras and new software tools leverage drones’ power to deliver a greater quantity and quality of information. There are promising efforts to develop low-cost drone technology that facilitate better biodiversity conservation results. For analysis, software tools are also improving to take advantage of increases in computing power and artificial intelligence programming. Our findings serve as an introduction to low-cost teledetection for biodiversity conservation, with drones providing an advantage in monitoring habitats by increasing information quantity, accuracy, and ease of analysis with the aid of automation.
在信息丰富的时代,智慧已经成为一种稀缺资源。对生物多样性的探索和测量产生了大量的信息,这些信息按照生命本身的模式分成了许多类别和类型。我们的目标是通过使用低海拔、低成本的地球观测技术(如小型空中无人机)来评估绘制林地、草原、沼泽地和其他与保护相关的自然和半自然区域的植物多样性的潜力,这些技术可以捕获可以绘制和分析的图像。由于这项技术是新的,发展迅速,我们将搜索限制在2012-2019年之间发表的研究。我们已经在林业、农林复合、园艺、草原、生态修复等领域找到了应用。在技术进步方面,新的硬件组件和摄像头以及新的软件工具利用无人机的能力来提供更多数量和质量的信息。在开发低成本无人机技术以促进更好的生物多样性保护结果方面,人们做出了有希望的努力。在分析方面,软件工具也在不断改进,以利用计算能力和人工智能编程的提高。我们的研究结果可以作为生物多样性保护的低成本远程检测的介绍,无人机通过增加信息量,准确性和自动化分析的便利性,在监测栖息地方面具有优势。
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引用次数: 0
期刊
Bulletin of the University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca
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