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Isolated Microorganisms for Bioconversion of Biodiesel-Derived Glycerol Into 1,3-Propanediol 生物柴油衍生甘油转化为1,3-丙二醇的分离微生物
L. Mitrea, L. Călinoiu, Gabriela Precup, M. Bindea, B. Rusu, M. Trif, B. Ștefănescu, I. Pop, D. Vodnar
During biodiesel production, massive amounts of raw glycerol are created generating an environmental issue and the same time an increase of biodiesel production cost at the same time. This raw glycerol could be converted by specific strains into value-added products, like 1,3-propanediol (1,3-PD), an important monomer used in the synthesis of biodegradable polyesters. The present work is based on recent scientific articles and experimental studies on the targeted topic, namely on the use of bacterial strains for bioconversion of biodiesel-derived glycerol into valuable products, like 1,3-PD. Concentrations, yields and productivity of 1,3-PD are presented for various bacterial strains. Important results as respects the microbial bioconversion of biodiesel-derived glycerol into 1,3-PD were registered for strains like Klebsiella pneumoniae, Citrobacter freundii, Escherichia coli and Lactobacillus diolivorans. From this study can be concluded that waste glycerol may be used as a nutrient source for microbial development and the production of 1,3-propanediol with high concentrations and yields.
在生物柴油的生产过程中,产生了大量的原料甘油,产生了环境问题,同时也增加了生物柴油的生产成本。这种原料甘油可以通过特定菌株转化为增值产品,如1,3-丙二醇(1,3- pd),这是合成生物可降解聚酯的重要单体。目前的工作是基于最近的科学文章和实验研究的目标主题,即利用细菌菌株将生物柴油衍生的甘油生物转化为有价值的产品,如1,3- pd。介绍了不同菌株的1,3- pd的浓度、产率和生产效率。在肺炎克雷伯菌、弗氏柠檬酸杆菌、大肠杆菌和diolivorans等菌株中将生物柴油衍生甘油转化为1,3- pd方面取得了重要的微生物转化结果。研究结果表明,废甘油可作为微生物发育的营养源,并可高产、高浓度地生产1,3-丙二醇。
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引用次数: 19
The suitability and the bio-chemical characteristics of soils from the Northwest Region of Development from Romania, for blueberry crop. 罗马尼亚西北发展区土壤对蓝莓作物的适宜性及生化特性。
A. Boț, I. Păcurar, L. Szajdak, S. Roșca
We conducted several bio-chemical analyses on soil samples collected from eight blueberry plantations from Northwest Region of Development, using such as Kjeldahl method for total N, Perucci, Krawczyński, Hoffman and Teicher methodology for determine soil enzyme. The bio-chemical analyses conducted provide a better qualitative characterization of soils, providing significant information regarding their fertility degree and favourability for blueberries. For instance, the higher amount of total organic carbon found in four of the eight studied plantation, can be correlated with the same higher amount of enzyme present in soil which have an influence on them, xanthine oxidase and peroxidase activity. Taking into account the requirements needed for a blueberry plantation, we demonstrate that organic soils have also had the highest amount of bio-chemical compounds, such as cambisoil, met in Galații Bistriței, where were also found the highest amounts of different forms of nitrogen (1.755 g/kg total N; 0.0189 g/kg NH4 and 0.0231g/kg NO3 in Galații Bistriței, comparing to 1.344 g/kg of total nitrogen; 0.0126 g/ kg NH4 and 0.0119 g/kg NO3 in Ulmeni), different forms of carbon and the enzymes that influence this compounds in soil, xanthine oxidase activity, peroxidase activity and nitrate reductase activity.
采用凯氏定氮法测定全氮,Perucci, Krawczyński法测定土壤酶,Hoffman和Teicher法测定土壤酶,对西北开发区8个蓝莓种植园土壤样品进行了生化分析。所进行的生物化学分析提供了更好的土壤定性特征,提供了关于其肥力程度和蓝莓有利度的重要信息。例如,在研究的8个人工林中,有4个人工林的总有机碳含量较高,可能与土壤中存在的酶的含量相同较高有关,这些酶对它们的黄嘌呤氧化酶和过氧化物酶活性有影响。考虑到蓝莓种植园所需的要求,我们证明有机土壤也具有最高数量的生化化合物,如cambisoil,在Galații Bistriței中得到满足,在那里也发现了最高数量的不同形式的氮(1.755 g/kg总氮;Galații Bistriței中NH4为0.0189 g/kg, NO3为0.0231g/kg,总氮为1.344 g/kg;0.0126 g/kg NH4和0.0119 g/kg NO3 (Ulmeni),土壤中不同形式的碳和影响这些化合物的酶,黄嘌呤氧化酶活性,过氧化物酶活性和硝酸盐还原酶活性。
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引用次数: 0
Characterization of essential oils extracted from different aromatic plants by FTIR spectroscopy. 不同芳香植物精油的傅里叶红外光谱表征。
M. Morar, Florinela Fetea, A. Rotar, M. Nagy, C. Semeniuc
Four essential oils isolated from dried leaves of parsley, lovage, basil and thyme were investigated by FTIR spectroscopy. The analysis of recorded spectra revealed that the main compounds of tested essential oils dominate their vibrational spectra.
用红外光谱法研究了从欧芹、罗勒、罗勒和百里香干叶中分离得到的四种精油。对记录的光谱分析表明,所测精油的主要化合物在其振动光谱中占主导地位。
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引用次数: 15
Obtaining a Functional Product Through the Exploitation of Mushroom Flour in Pasta 利用蘑菇粉开发功能性面食产品
C. Mureșan, A. Fǎrcaş, S. Man, Ramona Suharoschi, R. Vlaic
The present study focuses on the influence of adding mushroom flour on a certain assortment of pasta. The research comprises two major directions: the study of the raw materials used to produce pasta from a compositional point of view and the quantification of some biologically active compounds of interest; emphasizing the potential of using Boletus edulis mushroom flour in the composition of pasta. For this purpose, two types of pasta have been created, with different percentages of mushroom, 10% and 20%, but also a blank sample obtained in the same conditions, but without mushroom flour. To achieve the goal the following analyzes were conducted: proteins, total polyphenols, antioxidant activity, fat, humidity, ashes, acidity, increase in volume of the boiled pasta and customers’ preferences. By using acceptability test performed on the 9-point hedonic test has been established that the consumers preferred pasta enriched with 10% mushroom flour. The present results are therefore helping the food industry regarding product diversity, consumers being eager to consume these products which have nutritional value and functional proprieties
本研究的重点是添加蘑菇粉对某品种面食的影响。研究包括两个主要方向:从成分的角度研究用于生产面食的原材料,并对一些感兴趣的生物活性化合物进行量化;强调了在意大利面中使用肉毒杆菌蘑菇粉的潜力。为此,我们制作了两种不同比例的蘑菇面,分别为10%和20%,以及在相同条件下获得的空白样品,但没有蘑菇粉。为了达到这个目标,我们进行了以下分析:蛋白质、总多酚、抗氧化活性、脂肪、湿度、灰分、酸度、煮面食体积的增加和顾客的偏好。通过对9点享乐性测试进行可接受性测试,确定了消费者对添加10%蘑菇粉的面食的偏好。因此,目前的结果有助于食品工业的产品多样性,消费者渴望消费这些具有营养价值和功能特性的产品
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引用次数: 4
Characterization Phyto-Socio-Ecological of Agrostis capillaris L. Grasslands 毛缕草草地植物社会生态特征
Mirela Cirebea, I. Rotar, R. Vidican, F. Păcurar, A. Malinas, O. Ranta
Floristic composition of the pastures is a mirror action and practical factors applied station and management (maintenance and how to use). The purpose of this research is to assess the state of the biodiversity and pastoral value for Agrostis capillaries L. grasslands from the mountain area. Also, there has been analyzed the floristic composition and a series of ecological indexes, respectively humidity, soil reaction, temperature, and nitrogen. We examined how estimated species diversity patterns changed with varying survey intensity from BAƒiE™oara village, Cluj County, Romania, an Agrostis capillaries L. grasslands. The experiment was performed in 2015. The vegetation observations were made on 29 plots. In this descriptive plot been identified the type of grassland Agrostis capillaries , described in boreal at altitudes between 850 and 1200, generally starting on exhibitions east until the south (90Es - 180 Es).The floristic composition of the Agrostis capillaries grasslands from the studied area is influenced by the local conditions and the intensity of the exploitation, these surfaces being under-exploited .
牧草的植物区系组成是一个镜像作用和实际因素,适用于站位和管理(维护和如何利用)。本研究的目的是评估山区毛缕草草地的生物多样性状况和放牧价值。此外,还分析了植物区系组成和湿度、土壤反应、温度、氮等一系列生态指标。我们研究了在罗马尼亚克鲁日县BAƒiE™oara村的一个Agrostis capillaries L.草地上,估计的物种多样性模式是如何随着调查强度的变化而变化的。该实验于2015年进行。对29个样地进行了植被观测。在这幅描述图中,确定了草地Agrostis毛细管的类型,描述了北纬850至1200之间的海拔高度,通常从东部开始直到南部(90Es - 180es)。研究区毛细草的植物区系组成受当地条件和开发强度的影响,这些表面处于未开发状态。
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引用次数: 0
Impact on the Abandonment of Semi-Natural Grasslands from Apuseni Mountains 对阿普塞尼山地半自然草原撂荒的影响
I. Vaida, I. Rotar, F. Păcurar, R. Vidican, A. Pleșa, A. Malinas, V. Stoian
According to The European Communion in Europe there are large areas of abandoned grasslands (Osterburg and colab., 2010). The abandonment of grasslands entails substantial changes to the vegetation cover. Usually, after a period of time, semi-natural grasslands start to develop forestlike features. In Romania despite the existence of a subsidiary system large areas with semi-natural grasslands are abandoned. The objective of this paper was to analyze the effect of abandonment on the structure of vegetation cover. The experiment consists in 3 experimental variants (1 mowed annually, 1 abandoned for 5 years and 1 abandoned for 15 years) and is located in the village of GheE›ari, from GA¢rda de Sus commune at an altitude of 1130 m. Vegetation was studied using the Braun-Blanquet method, modified by PAƒcurar and Rotar in 2014. The scientific results were processed with the numerical analysis application (Cluster Analyses) belonging to PC-ORD program. Results showed that the grassland abandoned for 5 years did not show any statistical difference compared to the grassland mowed annually. The grassland abandoned for 15 years showed modification in the structure of vegetation cover translated by the disappearance of numerous species.Our reccomandation is that semi-natural grasslands, which have numerous economical disadvantages because of their location, should be mowed at least every five years.
根据欧共体在欧洲有大面积的废弃草原(Osterburg和colab)。, 2010)。草原的废弃导致植被覆盖发生重大变化。通常,经过一段时间后,半自然草原开始发展出类似森林的特征。在罗马尼亚,尽管存在一个附属系统,但大面积的半天然草原被遗弃。本文的目的是分析撂荒对植被覆盖结构的影响。该试验包括3个试验变种(1个每年刈割,1个废弃5年,1个废弃15年),试验地点位于海拔1130米的GA ø rda de Sus公社GheE ari村。植被研究采用Braun-Blanquet方法,并于2014年通过PAƒcurar和Rotar进行了改进。用PC-ORD程序中的数值分析应用程序(聚类分析)对科学结果进行处理。结果表明,撂荒5年的草地与每年刈割的草地没有统计学差异。撂荒15年的草原,由于大量物种的消失,植被覆盖结构发生了变化。我们的建议是,半天然草地应该至少每五年修剪一次,因为它们的位置有很多经济上的缺点。
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引用次数: 0
The effect of fertilizer regime on soil fauna. 施肥制度对土壤动物的影响。
M. Șandor, T. Brad, A. Maxim, V. Șandor, B. Onica
Soil fauna activity in agricultural soil is a key factor to maintain soil fertility and to assure soil ecosystem services. It is now accepted that agricultural practices like tillage and pesticide use can harm soil organisms including earthworms and springtails. Other practices like the use of green manure or animal manure have been considered as being beneficial to these soil invertebrates. To deepen our knowledge on the effect of fertilizers (mineral and organic) on earthworms ( Aporrectodea caliginosa and Lumbricus terrestris ) and springtails  ( Folsomia candida) 56 microcosm experiments were made with two soil types and two hydric regimes. The microcosms were amended with four fertilizers: ammonium nitrate, mustard as green manure, cow manure and slurry. The results emphasize that mustard use had beneficial effect on Folsomia candida abundance and Aporrectodea caliginosa biomass, while mineral fertilizer had negative effects for all species used in the experiment.
农业土壤中土壤动物活动是维持土壤肥力和保证土壤生态系统服务的关键因素。现在人们普遍认为,耕作和使用农药等农业做法会损害土壤生物,包括蚯蚓和跳尾虫。其他做法,如使用绿肥或动物粪便被认为对这些土壤无脊椎动物有益。为了加深对肥料(无机和有机)对蚯蚓(Aporrectodea caliginosa)和蚯蚓(Lumbricus terrestris)和弹尾(Folsomia candida)的影响的认识,在两种土壤类型和两种水分制度下进行了56个微观实验。用硝酸铵、芥菜作绿肥、牛粪和浆料4种肥料对微生物进行改良。结果表明,施用芥菜对假丝酵母菌的丰度和绿僵霉的生物量有显著的促进作用,而施用矿肥对试验中所有物种均有不利影响。
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引用次数: 4
Influence of Climate Factors and Plant Varieties on the Hay Technology (Fibrous Feed) 气候因子和植物品种对干草技术(纤维饲料)的影响
V. Bărbieru, M. Olar, O. Ranta, M. Muntean, O. Marian, L. Ognean, Georgiana Cătunescu
The present study was carried on the Research and Didactical Station Jucu located close to SomeAŸ River. The aim of the study is to determine the fuel consumption and productivity of aggregates (machineries) used in the conditions of the two levels of hay production technology. The experimental plot uses Festuca Arundinacea , while control, spontaneous vegetation ( Dactilys glomerata 70%, Alopecurus pratensis 10% Festuca sp .6%, Trifolium repens 5%, other plants 9%). Grassland soil taken in study type is molded with a high coefficient of withering (16-19%) and a small capacity water output (8%). Chemical characteristics of soil samples shows us a good content in humus and good to very good in mobile potassium, being deficient in phosphorus mobile. The differences noted between the fuel consumptions for the two parcels is due to higher vegetable production of control plot which has also a running speed of different aggregates also influencing effective productivity.
本研究是在位于SomeAŸ河附近的聚沽研究教学站进行的。该研究的目的是确定在两个水平的干草生产技术条件下使用的集料(机械)的燃料消耗和生产率。试验区以羊茅为主,对照区以自然植被为主(冬青70%,草皮10%,羊茅6%,三叶草5%,其他植物9%)。研究类型的草地土壤具有高枯干系数(16-19%)和小容量出水量(8%)的特点。土壤样品的化学特征表明,腐殖质含量好,流动钾含量好至极好,流动磷缺乏。两个地块燃料消耗之间的差异是由于对照地块的蔬菜产量较高,而且不同的团聚体运行速度也影响有效生产力。
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引用次数: 0
The Effects of Phosphate Solubilizing Rhizobacteria on Soybean (Glycine max. L.) Plants Grown under Insoluble Phosphate Fertilization 磷酸增溶根杆菌对大豆甘氨酸最大值的影响。l .)不溶性磷酸盐施肥下生长的植物
V. Rotaru
The aim of this study was to assess the comparative efficacy of two phosphorus solubilizing rhizobacteria namely Burkholderia cepacia B36 and Enterobacter radicincitans D5/23T combined with insoluble phosphates in soybean ( Glycine max . L.). Inoculated plants were grown in sand culture under controlled greenhouse conditions. All the inoculated treatments showed better plant growth and nutrient uptake when compared to uninoculated control. The inoculation with B. cepacia performed better than with E. radicincitans . Phosphorus concentrations in shoots and roots did not change significantly at the early stage of plant growth. However, the bacterial inoculation had better stimulatory effect on phosphorus uptake by soybean fertilized with insoluble phosphates, in particular in treatment with combined application of both bacteria strains.
本研究的目的是评价两种溶磷根瘤菌即洋葱伯克霍尔德菌B36和根瘤菌D5/23T与大豆(甘氨酸max .)中不溶性磷酸盐结合的比较效果。l .)。接种植株在受控温室条件下进行沙培养。与未接种对照相比,所有接种处理均表现出较好的植株生长和养分吸收。接种洋葱芽孢杆菌的效果优于接种根茎芽孢杆菌。植株生长初期,根系和茎部磷含量变化不显著。但接种细菌对不溶性磷酸盐对大豆磷吸收的促进作用较好,特别是两种细菌联合施用的效果更好。
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引用次数: 0
Mineral Fertilization with UAN on Natural Grassland Festuca rubra L. with Agrostis capillaries L. 天然草地红羊茅与毛缕草的氮素施肥研究。
I. Rotar, Mirela Cirebea, R. Vidican, F. Păcurar, A. Malinas, O. Ranta
An important part of efficient livestock production is ensuring the sufficient grass for hay and pasture. However, low soil nutrient levels often limit forage production. With good fertilizer management and soil fertility, the productivity of many hay and pasture fields can be greatly improved. Through good fertilizer management, the productivity of many hay and pasture fields can be significantly improved by Ross H. McKenzie (2005). The aim of this paper was the effect of fertilization with liquid fertilizer (UAN) the harvest of dry and floristic composition changing on natural grassland. The experiment whose results we present was placed in 2014 in the place in Baisoara Mountain village, Cluj County. Experience has been placed on the Festuca rubra and Agrostis capillaries - of grassland type. The natural grassland of Festuca rubra with Agrostis capillaries responded very well to mineral fertilizers with liquid fertilizer UAN. The floristic composition of natural grassland fertilized with liquid fertilizers based on nitrogen, it can be seen an increase the Gramineae families and an evidence downward trend from Fabaceae families.
高效畜牧业生产的一个重要组成部分是确保有足够的干草和牧草。然而,土壤养分水平低往往限制饲料生产。通过良好的肥料管理和土壤肥力,许多干草和牧场的生产力可以大大提高。Ross H. McKenzie(2005)指出,通过良好的肥料管理,许多干草和牧场的生产力可以显著提高。以天然草地为研究对象,研究了水肥施肥对旱地收获和植物区系组成变化的影响。我们所展示的实验结果于2014年在克鲁日县白什瓦拉山村进行。对草地类型的红羊茅和毛细Agrostis进行了研究。含毛臭茅的天然草地对矿质肥和液体肥的反应很好。施用氮肥的天然草地植物区系组成中,禾本科增加,豆科明显下降。
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引用次数: 2
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Bulletin of the University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca
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