Current health sciences journal最新文献

Polymethyl methacrylate (PMMA) has been routinely used as bone cement, being well tolerated by most patients. The authors report the case of a 42-year-old patient that underwent cranioplasty with PMMA prosthesis, that reported progressive and persistent skin symptoms starting 45 days after surgery. Dermatologic evaluation revealed an extensive reddish scar plaque, associated with polytrichia and alopecia, located only in the left parieto-occipital region, above the PMMA cranioplasty. Scalp biopsy and bone cement specific patch test revealed both folliculitis decalvans (FD) and weak PMMA hypersensitivity. The patient was managed with dapsone for FD, and graft removal was not necessary. This is the second case of post-cranioplasty PMMA hypersensitivity, and the first report of potential contact dermatitis induced FD.

Non-alcoholic liver disease is affecting approximately a quarter of the global population, with a significant morbidity and mortality rate. The present paper takes into discussion a systematic review of the literature concerning the role that microRNAs (miRNAs) and epigenetic factors can have in relation with NAFLD. Using PRISMA methodology guidelines, the systematic review offers a comprehensive image of the relevant scientific literature that explains the complex role of miRNAs in relation with NAFLD. Throughout the process of reviewing there has been highlighted seven different subtopics that can synthetize the nature and the content of the different studies: the role of miRNAs in fibrosis and liver disease progression; use of microRNAs as biomarkers for diagnosing steatotic liver disease; microRNAs and targeted therapies for NAFLD (Nonalcoholic fatty liver disease) /NASH (Non-alcoholic Steatohepatitis); metabolic regulation and obesity via microRNAs; interaction of miRNAs with other factors at the molecular level in disease pathogenesis; the role of miRNAs in systemic inflammation and effects on various other organs and the role of miRNAs as regulators and highly specialized epigenetic factors affecting NAFLD pathogenesis and evolution. Overall, this review presents diverse approaches on microRNAs and gives a background for future applied research that can explain and test their potential as biomarkers for diagnosing and understanding disease mechanisms, particularly in populations exposed to historical nutritional imbalances.

Early pregnancy loss (EPL) is the most common form of miscarriage and establishing its exact etiology is vital for the proper prognosis and management of possible future pregnancies. The aim of our study was to assess the incidence and types of chromosome abnormalities in product-of-conception (POC) samples analyzed by Quantitative Fluorescent Polymerase Chain Reaction (QF-PCR). Two hundred fifty-seven POC samples meeting EPL criteria were referred to the Human Genomics Laboratory of the University of Medicine and Pharmacy of Craiova, Romania. DNA was extracted from both POC tissue and maternal blood samples, while PCR products were migrated on the ABI3730xl platform. A total of 124 samples were successfully analyzed, 46 cases (37.1%) showing various types of abnormalities, while no aneuploidies were found in the remaining 78 samples (62.9%). The most common abnormalities were monosomy X, followed by triploidy, trisomy 18, 16 and 15. The basic QF-PCR kit had an overall detection rate of 25.8%, but the detection rate rose to 37.1% when employing the extended kit. Our study proves that QF-PCR can be used as a first approach in the genetic analysis of POC, followed by conventional karyotyping (KT) or Chromosomal Microarray Analysis (CMA) as follow-up. QF-PCR is able to identify maternal cell contamination, as well as provide timely results.

Purpose: The aim of the study was to analyze the peri-implant clinical and radiological parameters and their correlations with the plaque index (mPI) and the inflammation index (GI) in fixed implant-prosthetic therapy.

Materials and method: This research was a retrospective study including 48 patients with posterior partial edentulism (age: mean 63.04±10.723 years) treated by implant-supported metal-ceramic bridges, with a mean 6.19 years follow-up. A total of 166 implants were evaluated by clinical (peri-implant pocket depth-PPD) and radiological (peri-implant marginal bone loss-MBL) examen. Plaque index (mPI) and inflammation index (GI) were recorded for each implant site. Mean values of PPD, MBL (mesial), MBL (distal) were assessed. A comparative analysis was conducted regarding relation between PPD and MBL values (distal and mesial) in relation to the mPI and GI indices using the non-parametric Mann-Whitney and Kruskal-Wallis tests. Correlations between the variables PPD, MBL (mesial), and MBL (distal) for the entire study group were calculated by Pearson linear correlation coefficient.

Results: There were very high positive correlations between pocket depth and mesial marginal bone loss (r=0.951, p<0.001; 95% CI: 0.934-0.964); pocket depth and distal marginal bone loss (r=0.961, p<0.001; 95% CI: 0.947-0.971), and mesial and distal bone loss values (r=0.935, p<0.001; 95% CI: 0.913-0.952).

Conclusion: The findings indicate that peri-implant bone loss and pocket depth are strongly correlated and significantly influenced by plaque accumulation and inflammation, highlighting the critical role of the patients' oral hygiene in maintaining peri-implant tissue health.

The aim of the study is to identify the dental somato-facial anomalies in young patients with early loss of temporary teeth, with the identification of crowding and other present clinical entities. The study was carried out on a working batch of 635 subjects who referred to the Pediatric Dentistry Clinic in Iasi and the private dental practice for various dental treatments, without any obvious dysfunctional subjective symptomatology. Out of 635 subjects,254 were female (40%) and 381 male patients (60%), distributed in the rural area (n=157; 24.7%) and urban area (n=478; 75.3%). By applying the selection criteria, the aim was to avoid issues of differential diagnosis with other disorders of the orofacial system. The mean age of the girls in the group was 8.26±1.840 years and the mean age of the boys was 8.77±1.856 years; the mean age of the rural children was 8.88±1.962 years, with an average of 8.46±1.823 years for the urban children. Dental malocclusions represent complex clinical entity, capable of producing functional, aesthetic and psychological disharmonies. Malocclusions can be caused by a combination of factors, in which the early loss of temporary teeth, the frequency of vicious habits, without excluding genetic determinism, are particularly important. The disorders that occur in the case of malocclusion may be aesthetic, masticatory, speech impairments, temporomandibular joint dysfunctions and social integration disorders.

Pineal cysts (PCs) are common findings on (Magnetic resonance Imaging) MRI, often incidental in females and asymptomatic throughout life. Rare complications, like pineal apoplexy with acute hydrocephalus, require differential diagnosis and urgent intervention. We report a 19-year-old male with a progressive headache and visual decline. MRI showed a 2.3 cm pineal cyst with hemorrhagic features (apoplexy) causing hydrocephalus. He underwent endoscopic third ventriculostomy and microsurgical resection, both successful. Postoperatively, symptoms resolved completely. Though often benign, complicated PCs can be life-threatening. This case highlights their management, aiding understanding of etiologies, differential diagnoses, and treatments, enhancing medical knowledge.

Postoperative acute pancreatitis can be a relatively common complication regarding abdominal surgery, especially for general surgeons who have a high suspicion and expertise in the specific field. After spine surgery, on the other hand, it is considered rare, and when it settles, it is non-negligible as it may progress to a life-threatening condition. Although there is a plethora of reports regarding postoperative acute pancreatitis after spine surgery, especially scoliosis correction surgery or extended fusion surgery, many spine surgeons do not acknowledge this rare complication. Factors that may favor the apparition of acute pancreatitis include intraoperative hypoperfusion, hemodynamic instability, correction techniques in spinal deformity conditions, and mechanical abdominal pressure due to the patient's position. We report a case of acute pancreatitis after posterior lumbar fixation and decompression for spinal canal stenosis.

In the last decade, demand for blood has been increased internationally, while there is a consistent shortage of regular blood donors. Medical students are a potential pool of regular blood donors. The present study investigated the attitudes and knowledge of undergraduate medical students, regarding blood donation. During the May of 2023 data from 320 medical students of the University of Crete, Greece who were studying from the first to the sixth year were analyzed. It was a cross-sectional observational study and data collection was performed by completing an anonymous questionnaire with socio-demographic items and the Blood Donation Attitudes and Knowledge Scale. Three hundred twenty undergraduate students participated in this study. Sophomores accounted for 20.3& (n=65). Only 25.9& of the participants donated blood repeatedly. Most students (99.4&; p<0.001) believed that donating blood was a good act and 87.5& reported that religious beliefs should not influence blood donation (both p<0.001). The frequency of students in the 4th-6th year who achieved a high score in Attitudes and Knowledge scale was higher compared to the 1st-3rd year students (23.6% vs. 10.6%, p=0.002). Higher score on Attitudes and Knowledge about Donation was significantly associated with increased year of study (r=0.196, p<0.001). Moderate levels of Attitudes and Knowledge Scale about Blood Donation were reported with a significant tendency to increase along the years of study. Perhaps, medical students may mature their perception towards blood donation alongside with their training advancement in Medicine.

Between January 2020 and December 2024, a significant number of burn patients were admitted to the Plastic Surgery Clinic of the Craiova Emergency Clinical Hospital. Due to the high incidence of burns requiring hospitalization and the limited number of beds available for such patients, it became essential to identify optimal treatment methods that could reduce hospital stay while ensuring favorable functional and aesthetic outcomes. Early debridement is considered a vital first step in the treatment of burn patients, ideally performed within the first few days post-injury. Burn treatment involves both surgical and systemic approaches. Surgical management includes debridement, skin grafting, and reconstruction. Tangential surgical debridement remains the gold standard for deep burns, allowing precise removal of necrotic tissue while preserving viable skin. However, it may be associated with disadvantages such as intraoperative blood loss and incomplete excision. Alternative methods have evolved, such as hydrodissection, which uses a high-pressure saline jet for selective debridement, particularly useful in partial thickness burns. Larval therapy (using sterile Lucilia sericata maggots) is another option, especially effective in infected or necrotic wounds, offering antimicrobial benefits as well. Enzymatic debridement, particularly with bromelain-based products, is a modern, selective, and less invasive method that promotes healing with minimal bleeding and often reduces the need for grafting. A clinical case from Craiova highlights the success of enzymatic debridement: a 75-year-old patient with extensive burns showed significant improvement and near-complete epithelialization after 24 days, without requiring grafts. These outcomes support the growing use of enzymatic agents as a valuable tool in modern burn management, offering rapid, effective treatment and better patient recovery.

Thyroid surgery is nowadays a mostly safe and standardized procedure, with low complication and mortality rates. The specific complications of thyroid surgery are lesion of the recurrent laryngeal nerve (RLN), hypoparathyroidism, either transient or permanent, and hemorrhage that can be in some cases life-threatening; even if not, the impact on the quality of life can be negative. The identification of risk factors for complications of thyroid surgery and development of strategies for reducing morbidity are important for the thyroid surgeon. We conducted a retrospective study on a database of 60 patients operated for a thyroid disease in a period of two years in the setting of a non-specialized general emergency hospital in both general surgery department and thoracic surgery department. The main complication was transient hypoparathyroidism (defined by lower than 8mg/dl serum calcium postoperatively)-18.33% of the group, followed by cervical minor hematoma-5.00% of cases and RLN injury in one case-1.67%. Regression analysis showed type of thyroid surgery (p-value 0.0471), histopathology type of disease (p-value 0.0566) and difficulty of surgery defined by operative time (p-value 0.0494) as the risk factors for postoperative complications, of which only the last one can be modifiable by some extent. Identifying and minimizing the risk factors of difficult surgery might improve also complication rates.