Pub Date : 2021-05-14DOI: 10.15835/BUASVMCN-HORT:2020.0044
G. Negoșanu, C. Vinatoru, O. Agapie, E. Barcanu
The Breeding and Biodiversity Laboratory within the Vegetable Research and Development Station Buzău with 11 tomato varieties listed in the Official Catalogue of Cultivated Crops in Romania and a valuable resource of Solanum lycopersicum germplasm composed of over 2,000 genotypes, focused its research to obtain tomato varieties for precise use: fresh consumption and industrialization. Starting from a valuable traditional variety (‘Inimă de bou’ – ‘Ox Heart’), the aim was to obtain a new, improved variety of tomato for consumption of fresh fruits, with large, juicy fruits highly appreciated on local market. Research resulted in the approval and patenting of the ‘Andrada’ tomato variety with distinct genotypic and phenotypic characteristics.
{"title":"Main Phenotypic and Biochemical Characteristics Recorded at the New ‘Andrada’ Tomato Variety Obtained at Vegetable Research and Development Station Buzău","authors":"G. Negoșanu, C. Vinatoru, O. Agapie, E. Barcanu","doi":"10.15835/BUASVMCN-HORT:2020.0044","DOIUrl":"https://doi.org/10.15835/BUASVMCN-HORT:2020.0044","url":null,"abstract":"The Breeding and Biodiversity Laboratory within the Vegetable Research and Development Station Buzău with 11 tomato varieties listed in the Official Catalogue of Cultivated Crops in Romania and a valuable resource of Solanum lycopersicum germplasm composed of over 2,000 genotypes, focused its research to obtain tomato varieties for precise use: fresh consumption and industrialization. Starting from a valuable traditional variety (‘Inimă de bou’ – ‘Ox Heart’), the aim was to obtain a new, improved variety of tomato for consumption of fresh fruits, with large, juicy fruits highly appreciated on local market. Research resulted in the approval and patenting of the ‘Andrada’ tomato variety with distinct genotypic and phenotypic characteristics.","PeriodicalId":9406,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Horticulture","volume":"78 1","pages":"95"},"PeriodicalIF":0.0,"publicationDate":"2021-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45162261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-11-22DOI: 10.15835/buasvmcn-vm:2020.0004
Laatra Zemmouri, M. Besbaci, A. Mammeri, M. Lafri
The aim of this study was to evaluate the seroprevalence, risk factors and zoonotic threats of the major abortive bacterial agents in sheep of M’Sila Governorate. A total of 184 serum samples were collected from ewes among 16 sheep flocks and tested for Coxiella burnetti, Chlamydia abortus, Brucella spp. and Salmonella abortusovis via ELISA. Simultaneously, a questionnaire was used to collect breeding management data. Seropositive results were as follows : Coxiella burnetti (27.9%), Salmonella abortusovis (15.9%), Chlamydia abortus (10.9%) and Brucella spp. (3.8%).The use of univariate analysis and multivariate logistic regression showed a highly significant correlation between Coxiella burnetti seropositivity and presence of cats in farms (OR = 5.75; 95% CI = 1.86-19.9; p= 0.001), while promiscuity with newly introduced animals was associated to Chlamydia abortus seropositivity (OR=3.37; 95%CI=1.01-14.9; p= 0.04). Additionally to the economic losses, the dissemination of Coxiella burnetti and Brucella spp. presents uncontrollable zoonotic hazards.
{"title":"Sero-epidemiological Investigation of the Major Abortive Bacterial Agents in Ewes of M’Sila Governorate, Algeria","authors":"Laatra Zemmouri, M. Besbaci, A. Mammeri, M. Lafri","doi":"10.15835/buasvmcn-vm:2020.0004","DOIUrl":"https://doi.org/10.15835/buasvmcn-vm:2020.0004","url":null,"abstract":"The aim of this study was to evaluate the seroprevalence, risk factors and zoonotic threats of the major abortive bacterial agents in sheep of M’Sila Governorate. A total of 184 serum samples were collected from ewes among 16 sheep flocks and tested for Coxiella burnetti, Chlamydia abortus, Brucella spp. and Salmonella abortusovis via ELISA. Simultaneously, a questionnaire was used to collect breeding management data. Seropositive results were as follows : Coxiella burnetti (27.9%), Salmonella abortusovis (15.9%), Chlamydia abortus (10.9%) and Brucella spp. (3.8%).The use of univariate analysis and multivariate logistic regression showed a highly significant correlation between Coxiella burnetti seropositivity and presence of cats in farms (OR = 5.75; 95% CI = 1.86-19.9; p= 0.001), while promiscuity with newly introduced animals was associated to Chlamydia abortus seropositivity (OR=3.37; 95%CI=1.01-14.9; p= 0.04). Additionally to the economic losses, the dissemination of Coxiella burnetti and Brucella spp. presents uncontrollable zoonotic hazards.","PeriodicalId":9406,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Horticulture","volume":"77 1","pages":"24"},"PeriodicalIF":0.0,"publicationDate":"2020-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41857727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-11-22DOI: 10.15835/buasvmcn-vm:2020.0032
A. Chende, V. Rus, C. Martonoș, Dalma Pivariu, A. Damian, V. Miclăuș, A. Gal
Stomach fragments from 3 guinea pigs were collected from the three regions: cardiac, fundic and pyloric, for histochemical investigations. The anatomical segments were processed by the classical method of inclusion in paraffin and the histological sections were stained with PAS reaction for highlighting the neutral mucins and the Alcian blue method for acid mucins. All the surface cells of the gastric mucosa and in the crypts were positive on the PAS reaction and negative in the case of the alcian blue reaction. This demonstrates that cells on the surface and in the crypts synthetize neutral mucins. In the case of the cardiac region glands, only a small number of cells were positive on the two histochemical reactions, which shows that the cardiac glands in guinea pig synthesize a very small amount of neutral and acidic mucins. There are no positive cells in neither reaction used in the fundic glands case, which shows that these glands do not synthesize mucins, neither neutral nor acidic content. The glands located in the pyloric region have cells in the deep half of the wall which were positive on both histochemical reactions, which shows that they synthesize both neutral mucins and acidic mucins.
{"title":"Histochemical Assessment of Mucin-Secreting Cells from the Gastric Mucosa of Guinea Pigs","authors":"A. Chende, V. Rus, C. Martonoș, Dalma Pivariu, A. Damian, V. Miclăuș, A. Gal","doi":"10.15835/buasvmcn-vm:2020.0032","DOIUrl":"https://doi.org/10.15835/buasvmcn-vm:2020.0032","url":null,"abstract":"Stomach fragments from 3 guinea pigs were collected from the three regions: cardiac, fundic and pyloric, for histochemical investigations. The anatomical segments were processed by the classical method of inclusion in paraffin and the histological sections were stained with PAS reaction for highlighting the neutral mucins and the Alcian blue method for acid mucins. All the surface cells of the gastric mucosa and in the crypts were positive on the PAS reaction and negative in the case of the alcian blue reaction. This demonstrates that cells on the surface and in the crypts synthetize neutral mucins. In the case of the cardiac region glands, only a small number of cells were positive on the two histochemical reactions, which shows that the cardiac glands in guinea pig synthesize a very small amount of neutral and acidic mucins. There are no positive cells in neither reaction used in the fundic glands case, which shows that these glands do not synthesize mucins, neither neutral nor acidic content. The glands located in the pyloric region have cells in the deep half of the wall which were positive on both histochemical reactions, which shows that they synthesize both neutral mucins and acidic mucins.","PeriodicalId":9406,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Horticulture","volume":"77 1","pages":"115"},"PeriodicalIF":0.0,"publicationDate":"2020-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47041911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-11-22DOI: 10.15835/buasvmcn-vm:2020.0008
Messaouda Taibi, Amel Benatallah, S. Zenia, M. Aissi, K. Harhoura, A. Milla, Amel Guerchaoui, Imene Kaabeche, Romeissa Khodja
Sarcosporidiosis is a parasitic disease due to the development of cystogenic coccidia of the genus Sarcocystis with dixene evolution and muscular localization, which can cause losses in cattle and generate an intestinal infection in domestic carnivores (dog, cat) and humans. The objective of our study was to determine the prevalence of Sarcocystis spp on 149 bovine carcasses at the Eucalyptus slaughterhouse in Algiers by macroscopic and microscopic examination and to evaluate the influence of certain risk factors (age, sex, breed and origin). Samples of esophagus and diaphragms were taken from slaughtered cattle and analyzed by enzymatic (pepsin) digestion and histological technique. The macroscopic examination was negative during carcasses inspection while the microscopic examination showed a prevalence of 100% for enzymatic digestion by revealing bradyzoites in the form of bananas and 47.7% at the histology with the detection of thin-walled cysts of S. cruzi (98.6%), of thickwalled cysts of S. hominis (15.5%) which was the zoonotic species and of S. hirsuta (1.39%). The risk factors studied significantly influenced the prevalence of Sarcocystis sp. This study revealed for the first time in the diaphragm a cyst of Sarcocystis hirsuta.
{"title":"Prevalence of Sarcosporidiosis in Carcasses of Cattle Slaughtered at the Eucalyptus Slaughterhouse-Algeria","authors":"Messaouda Taibi, Amel Benatallah, S. Zenia, M. Aissi, K. Harhoura, A. Milla, Amel Guerchaoui, Imene Kaabeche, Romeissa Khodja","doi":"10.15835/buasvmcn-vm:2020.0008","DOIUrl":"https://doi.org/10.15835/buasvmcn-vm:2020.0008","url":null,"abstract":"Sarcosporidiosis is a parasitic disease due to the development of cystogenic coccidia of the genus Sarcocystis with dixene evolution and muscular localization, which can cause losses in cattle and generate an intestinal infection in domestic carnivores (dog, cat) and humans. The objective of our study was to determine the prevalence of Sarcocystis spp on 149 bovine carcasses at the Eucalyptus slaughterhouse in Algiers by macroscopic and microscopic examination and to evaluate the influence of certain risk factors (age, sex, breed and origin). Samples of esophagus and diaphragms were taken from slaughtered cattle and analyzed by enzymatic (pepsin) digestion and histological technique. The macroscopic examination was negative during carcasses inspection while the microscopic examination showed a prevalence of 100% for enzymatic digestion by revealing bradyzoites in the form of bananas and 47.7% at the histology with the detection of thin-walled cysts of S. cruzi (98.6%), of thickwalled cysts of S. hominis (15.5%) which was the zoonotic species and of S. hirsuta (1.39%). The risk factors studied significantly influenced the prevalence of Sarcocystis sp. This study revealed for the first time in the diaphragm a cyst of Sarcocystis hirsuta.","PeriodicalId":9406,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Horticulture","volume":" ","pages":"54"},"PeriodicalIF":0.0,"publicationDate":"2020-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.15835/buasvmcn-vm:2020.0008","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46192171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-11-22DOI: 10.15835/buasvmcn-vm:2020.0018
M. Matei, C. Popovici, Daniela Neagu, V. Buza, A. Szakacs, L. Ștefănuț
The aim of the study was to assess the effect of a probiotic product based on Bacillus subtilis, Bacillus licheniformis and Pediococcus acidilactici on hematological parameters for dogs with apparent intestinal dysbiosis. Also, a comparison between the results obtained before and after the treatment was made, in order to establish if the probiotic treatment is able to improve the hematological results for dogs with apparent dysbiosis. A total number of 5 dogs, aged between 7 months and 6 years old were clinically evaluated for gastro-intestinal problems that lead to the suspicion of apparent intestinal dysbiosis. The probiotic administration respected the producer recommendations and it was given to the dogs together with their usual food, one capsule/day, 30 days consecutively. On the first and 31 days of the study, blood samples were collected and hematological parameters were evaluated. A heterogeneity in the hematological results was observed. However, when results from day 0 were compared with results obtained in day 31, the variance was considered to be physiological. Also, the small diferences between the values before and after the treatment can not be attributed only to the probiotic treatment. The results obtained regarding the dynamics of the hematological parameters provided data that sustain the benefic effect of this probiotic combination, without a direct impact on hematological parameters.
{"title":"Hematological Aspects on Dogs with Apparent Dysbiosis after Bacillus Subtilis, Bacillus Licheniformis and Pediococcus Acidilactici Probiotic Administration- Pilot Study","authors":"M. Matei, C. Popovici, Daniela Neagu, V. Buza, A. Szakacs, L. Ștefănuț","doi":"10.15835/buasvmcn-vm:2020.0018","DOIUrl":"https://doi.org/10.15835/buasvmcn-vm:2020.0018","url":null,"abstract":"The aim of the study was to assess the effect of a probiotic product based on Bacillus subtilis, Bacillus licheniformis and Pediococcus acidilactici on hematological parameters for dogs with apparent intestinal dysbiosis. Also, a comparison between the results obtained before and after the treatment was made, in order to establish if the probiotic treatment is able to improve the hematological results for dogs with apparent dysbiosis. A total number of 5 dogs, aged between 7 months and 6 years old were clinically evaluated for gastro-intestinal problems that lead to the suspicion of apparent intestinal dysbiosis. The probiotic administration respected the producer recommendations and it was given to the dogs together with their usual food, one capsule/day, 30 days consecutively. On the first and 31 days of the study, blood samples were collected and hematological parameters were evaluated. A heterogeneity in the hematological results was observed. However, when results from day 0 were compared with results obtained in day 31, the variance was considered to be physiological. Also, the small diferences between the values before and after the treatment can not be attributed only to the probiotic treatment. The results obtained regarding the dynamics of the hematological parameters provided data that sustain the benefic effect of this probiotic combination, without a direct impact on hematological parameters.","PeriodicalId":9406,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Horticulture","volume":"77 1","pages":"72"},"PeriodicalIF":0.0,"publicationDate":"2020-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47990426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-11-22DOI: 10.15835/buasvmcn-vm:2020.0020
D. Ancuta, C. Coman, D. Alexandru, M. Crivineanu
In order to investigate the interaction between the bone and the dental implant, in vivo testsare performed in accordance with the biocompatibility requirements. As in vitro tests do not provide sufficient data on the quality of the implants, the animal models are used because they allow a complete assessment of all the factors involved in the osseointegration process. The aim of the paper is to provide data on the bone structure of animal species involved in dental research, highlighting the complexity of bone structure and osseointegration depending on the species. At the same time, the importance of selecting animal models is emphasized, in order to obtain the most accurate data for the development of dental implants and their use in humans. Following the research, essential data were obtained that helped to synthesize the bone characteristics of animals such as rats, rabbits, pigs, sheep or goats and relate them to the human bone skeleton. These data are useful in choosing an animal model when testing a new dental implant.
{"title":"Animal Models Used in Testing the Biocompatibility of the Dental Implant – A Review","authors":"D. Ancuta, C. Coman, D. Alexandru, M. Crivineanu","doi":"10.15835/buasvmcn-vm:2020.0020","DOIUrl":"https://doi.org/10.15835/buasvmcn-vm:2020.0020","url":null,"abstract":"In order to investigate the interaction between the bone and the dental implant, in vivo testsare performed in accordance with the biocompatibility requirements. As in vitro tests do not provide sufficient data on the quality of the implants, the animal models are used because they allow a complete assessment of all the factors involved in the osseointegration process. The aim of the paper is to provide data on the bone structure of animal species involved in dental research, highlighting the complexity of bone structure and osseointegration depending on the species. At the same time, the importance of selecting animal models is emphasized, in order to obtain the most accurate data for the development of dental implants and their use in humans. Following the research, essential data were obtained that helped to synthesize the bone characteristics of animals such as rats, rabbits, pigs, sheep or goats and relate them to the human bone skeleton. These data are useful in choosing an animal model when testing a new dental implant.","PeriodicalId":9406,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Horticulture","volume":" ","pages":"1"},"PeriodicalIF":0.0,"publicationDate":"2020-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.15835/buasvmcn-vm:2020.0020","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42937417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-11-22DOI: 10.15835/buasvmcn-vm:2019.0040
S. Haffaf, Fatima Zahra Ragoub, A. Mammeri, Zouina Nouari, I. Djaalab, Abdessalam Zernine
Hydatidosis is a parasitic disease caused by Echinococcus granulosus. It is also among the most prevalent zoonotic diseases with special importance for public health that causes drastic financial losses in both developed and developing countries. Among Algerian governorates, M’Sila region includes an important number of sheep flocks. Thus, an epidemiological survey was conducted on sheep hydatidosis during 5 months between December 2017 and April 2018 at three slaughterhouses of M’sila governorate, situated in municipalities of M’Sila, Boussaâda and Sidi Aissa. In parallel, an epidemiological investigation via interviews and questionnaire was conducted from March to April, 2018 on a sample of 46 sheep breeders located in M’Sila region. The questionnaire treated several risk factors (presence and deworming of dogs, informal slaughtering practices, fiability of preventive measures... etc.). Results showed a general prevalence of 24.80% among 9048 slaughtered sheep, and a seasonal prevalence higher in spring (42.02%) than in winter (21.65%) (p>0.05). Sheep of five to six years old and more are the most parasitized (p<0.0001). The lungs (38. 24%) and the livers (30.39%) were the most affected organs, while 31.24% of sheep were affected simultaneously in both organs (liver and lungs). Furthermore, females are more affected (97.99%) than males (2.01%) (p<0.0001).
{"title":"Epidemiological Study of Hydatidosis in Sheep Slaughtered at M’Sila Abattoirs (Algeria)","authors":"S. Haffaf, Fatima Zahra Ragoub, A. Mammeri, Zouina Nouari, I. Djaalab, Abdessalam Zernine","doi":"10.15835/buasvmcn-vm:2019.0040","DOIUrl":"https://doi.org/10.15835/buasvmcn-vm:2019.0040","url":null,"abstract":"Hydatidosis is a parasitic disease caused by Echinococcus granulosus. It is also among the most prevalent zoonotic diseases with special importance for public health that causes drastic financial losses in both developed and developing countries. Among Algerian governorates, M’Sila region includes an important number of sheep flocks. Thus, an epidemiological survey was conducted on sheep hydatidosis during 5 months between December 2017 and April 2018 at three slaughterhouses of M’sila governorate, situated in municipalities of M’Sila, Boussaâda and Sidi Aissa. In parallel, an epidemiological investigation via interviews and questionnaire was conducted from March to April, 2018 on a sample of 46 sheep breeders located in M’Sila region. The questionnaire treated several risk factors (presence and deworming of dogs, informal slaughtering practices, fiability of preventive measures... etc.). Results showed a general prevalence of 24.80% among 9048 slaughtered sheep, and a seasonal prevalence higher in spring (42.02%) than in winter (21.65%) (p>0.05). Sheep of five to six years old and more are the most parasitized (p<0.0001). The lungs (38. 24%) and the livers (30.39%) were the most affected organs, while 31.24% of sheep were affected simultaneously in both organs (liver and lungs). Furthermore, females are more affected (97.99%) than males (2.01%) (p<0.0001).","PeriodicalId":9406,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Horticulture","volume":"77 1","pages":"7"},"PeriodicalIF":0.0,"publicationDate":"2020-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.15835/buasvmcn-vm:2019.0040","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41658865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-11-22DOI: 10.15835/buasvmcn-vm:2020.0010
I. Rățoi, L. Crivei, D. Porea, A. Dascalu, A. Cozma, L. Oslobanu, D. Anita, A. Aniță, G. Savuta
The present study aimed at identifying Mycobacterium avium seroprevalence in wild boars (Sus scrofa) and red foxes (Vulpes vulpes) samples, from the Eastern region of Romania, by using an indirect ELISA assay. A total of 367 wild animal samples were collected from eight counties: 275 wild boars sera samples from Iasi (n=209), Botosani (n=11), Bacau (n=17), Galati (n=27) and Covasna (n=11) and 92 fox samples of thoracic fluid from Iasi (n=30), Suceava (n=20), Neamt (n=19), Vaslui (n=11) and Galati (n=12). Specific antibodies were not detected in wild boar serum samples tested. From the fox samples, one was positive (1.08%), originating from Galati County. Our results may be correlated with a reduced distribution of Mycobacterium avium subspecies in the environment and a low prevalence of infections caused by these bacteria in wild animals. The current assessment shows that foxes have a limited role in the epidemiology of nontuberculous mycobacterial infections.
{"title":"Preliminary Seroepidemiological Investigation Regarding Mycobacterium Avium Infection in Wild Boars and Foxes","authors":"I. Rățoi, L. Crivei, D. Porea, A. Dascalu, A. Cozma, L. Oslobanu, D. Anita, A. Aniță, G. Savuta","doi":"10.15835/buasvmcn-vm:2020.0010","DOIUrl":"https://doi.org/10.15835/buasvmcn-vm:2020.0010","url":null,"abstract":"The present study aimed at identifying Mycobacterium avium seroprevalence in wild boars (Sus scrofa) and red foxes (Vulpes vulpes) samples, from the Eastern region of Romania, by using an indirect ELISA assay. A total of 367 wild animal samples were collected from eight counties: 275 wild boars sera samples from Iasi (n=209), Botosani (n=11), Bacau (n=17), Galati (n=27) and Covasna (n=11) and 92 fox samples of thoracic fluid from Iasi (n=30), Suceava (n=20), Neamt (n=19), Vaslui (n=11) and Galati (n=12). Specific antibodies were not detected in wild boar serum samples tested. From the fox samples, one was positive (1.08%), originating from Galati County. Our results may be correlated with a reduced distribution of Mycobacterium avium subspecies in the environment and a low prevalence of infections caused by these bacteria in wild animals. The current assessment shows that foxes have a limited role in the epidemiology of nontuberculous mycobacterial infections.","PeriodicalId":9406,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Horticulture","volume":"77 1","pages":"165"},"PeriodicalIF":0.0,"publicationDate":"2020-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67512073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-11-22DOI: 10.15835/buasvmcn-vm:2020.0009
D. Anita, Ioana Popescu, Ș. Anderco, L. Oslobanu, A. Aniță, G. Savuta
Culicoides is a genus of biting midges in the family Ceratopogonidae, representing a big threat, giving that several species serve as biological vectors of pathogens of veterinary importance. BTV distribution and persistence is thought to be almost entirely dependent upon the presence or absence of Culicoides adults vector. In this study, biting midges were collected during 2015-2016, once a week, from April to December. In the first year, 2617 BTV vector specimens were collected and identified as Culicoides obsoletus (41.2%; 95% CI: 39-43), Culicoides pulicaris (58%; 95% CI: 56-59) and Culicoides nubeculosus (0.8%; 95% CI: 0.4-1.1). In the second year, 165 BTV vector specimens were collected and identified as Culicoides obsoletus (44.25%; 95% CI: 36-51), Culicoides pulicaris (50.3%; 95% CI: 42-57), Culicoides dewulfi (4.84%; 95% CI: 1.5-8.1) and Culicoides nubeculosus (0.61%; 95% CI: 0.5-1.7). Culicoides pulicaris and Culicoides obsoletus complex were especially abundant within the two collection sites in Iași County. Our data on post-epidemic occurrence and composition of BTV vector species in the studied area will facilitate a better understanding of Culicoides vectors distribution and the possible role in bluetongue virus overwintering.
{"title":"Post-epidemic Occurrence of Bluetongue Virus Vectors (Culicoides Species) în Iași County","authors":"D. Anita, Ioana Popescu, Ș. Anderco, L. Oslobanu, A. Aniță, G. Savuta","doi":"10.15835/buasvmcn-vm:2020.0009","DOIUrl":"https://doi.org/10.15835/buasvmcn-vm:2020.0009","url":null,"abstract":"Culicoides is a genus of biting midges in the family Ceratopogonidae, representing a big threat, giving that several species serve as biological vectors of pathogens of veterinary importance. BTV distribution and persistence is thought to be almost entirely dependent upon the presence or absence of Culicoides adults vector. In this study, biting midges were collected during 2015-2016, once a week, from April to December. In the first year, 2617 BTV vector specimens were collected and identified as Culicoides obsoletus (41.2%; 95% CI: 39-43), Culicoides pulicaris (58%; 95% CI: 56-59) and Culicoides nubeculosus (0.8%; 95% CI: 0.4-1.1). In the second year, 165 BTV vector specimens were collected and identified as Culicoides obsoletus (44.25%; 95% CI: 36-51), Culicoides pulicaris (50.3%; 95% CI: 42-57), Culicoides dewulfi (4.84%; 95% CI: 1.5-8.1) and Culicoides nubeculosus (0.61%; 95% CI: 0.5-1.7). Culicoides pulicaris and Culicoides obsoletus complex were especially abundant within the two collection sites in Iași County. Our data on post-epidemic occurrence and composition of BTV vector species in the studied area will facilitate a better understanding of Culicoides vectors distribution and the possible role in bluetongue virus overwintering.","PeriodicalId":9406,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Horticulture","volume":"77 1","pages":"160"},"PeriodicalIF":0.0,"publicationDate":"2020-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67512434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-11-22DOI: 10.15835/buasvmcn-vm:2020.0034
T. Marcu, A. Gal, C. Martonoș, V. Rus, A. Damian, V. Miclăuș, A. Mihai
This study aimed to obtain as much and as accurate information as possible about the process of osseointegration of titanium implants, through histological processing. For this purpose, the combination of a mercury chloride fixator (Stieve mixture) and a complex staining method (Goldner’s trichrome) was used, which can highlight the microscopic structures in several colors and shades. The Stieve mixture proved to have distinguished qualities both in the preservation of basophilic and acidophilic structures and especially in the chemical preparation of the substrate for the binding of each structural component with a specific acidophilic dye. On the substrate thus prepared, the acidophilic dyes used in the Goldner’s trichrome staining highlighted the structural components in different colors and shades, offering the possibility of high precision assessment of the newly proliferated bone, in different stages of proliferation, consolidation and reshaping. Complex information was obtained, which allows the appreciation of osseointegration for endosseous implants and the amount of proliferated bone at the boneimplant interface, the stage of proliferation, consolidation and reshaping of the mature bone, the degree of fixation to deep bone structures. The possibility of complex assessment of the osseointegration process for endosseous implants at a given time is of great practical importance for correct assessment of the waiting time required for safe installation of prosthesis. The combination of Stieve fixator mixture with Goldner’s trichrome staining method has proven to be very effective in histology, highlighting on excellent level the existing structural components of each stage, in which the osseointegration process goes through. A very important role in this combination was achieved by mercury chloride mixture, which prepared the substrate for specific reaction with dyes. Regarding the results obtained, we recommend this combination for histological investigations aimed on bone proliferation and repair.
{"title":"Chemical Fixation Importance of Histologic Samples for Staining of Proliferated Bone During Implant Osseointegration","authors":"T. Marcu, A. Gal, C. Martonoș, V. Rus, A. Damian, V. Miclăuș, A. Mihai","doi":"10.15835/buasvmcn-vm:2020.0034","DOIUrl":"https://doi.org/10.15835/buasvmcn-vm:2020.0034","url":null,"abstract":"This study aimed to obtain as much and as accurate information as possible about the process of osseointegration of titanium implants, through histological processing. For this purpose, the combination of a mercury chloride fixator (Stieve mixture) and a complex staining method (Goldner’s trichrome) was used, which can highlight the microscopic structures in several colors and shades. The Stieve mixture proved to have distinguished qualities both in the preservation of basophilic and acidophilic structures and especially in the chemical preparation of the substrate for the binding of each structural component with a specific acidophilic dye. On the substrate thus prepared, the acidophilic dyes used in the Goldner’s trichrome staining highlighted the structural components in different colors and shades, offering the possibility of high precision assessment of the newly proliferated bone, in different stages of proliferation, consolidation and reshaping. Complex information was obtained, which allows the appreciation of osseointegration for endosseous implants and the amount of proliferated bone at the boneimplant interface, the stage of proliferation, consolidation and reshaping of the mature bone, the degree of fixation to deep bone structures. The possibility of complex assessment of the osseointegration process for endosseous implants at a given time is of great practical importance for correct assessment of the waiting time required for safe installation of prosthesis. The combination of Stieve fixator mixture with Goldner’s trichrome staining method has proven to be very effective in histology, highlighting on excellent level the existing structural components of each stage, in which the osseointegration process goes through. A very important role in this combination was achieved by mercury chloride mixture, which prepared the substrate for specific reaction with dyes. Regarding the results obtained, we recommend this combination for histological investigations aimed on bone proliferation and repair.","PeriodicalId":9406,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Horticulture","volume":"77 1","pages":"123"},"PeriodicalIF":0.0,"publicationDate":"2020-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45243238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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