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Pathomorphological Changes in Organs of Rabbits with Experimental Colibacillosis 实验性大肠杆菌病家兔脏器的病理形态学变化
M. Skrypka, O. Tul, B. Kyrychko
The aim of this research was to study pathomorphological alterations in the internal organs of rabbits through experimental reproduction of colibacillosis. The studies were conducted on 10 clinically healthy rabbits at the age of 3 months from a private farm. Animals were infected by intraperitoneal injection of a suspension of a pure culture of Escherichia coli isolated from a sand lizard. The disease of infected rabbits was accompanied by cachexia, dehydration and fever. The pathological process in the organism of infected animals was characterized by the violation of hemodynamics, the protein dystrophy of the parenchyma of liver, kidneys, myocardium and the formation of specific granulomas in the lungs and liver. The edema of reticular tissue and the hyperplasia of lymph nodes were observed in the organs of the immune system. The expressive edema of mucous membrane and submucosa, as well as alterative and necrotic processes were detected in the intestine.
本研究的目的是通过实验繁殖大肠杆菌病来研究家兔内脏的病理形态学变化。这项研究是在来自私人农场的10只3个月大的临床健康兔子身上进行的。通过腹膜内注射从沙蜥中分离的纯大肠杆菌培养物的悬浮液来感染动物。感染兔的疾病伴有恶病质、脱水和发烧。受感染动物体内的病理过程以血流动力学紊乱、肝、肾、心肌实质蛋白质营养不良以及肺和肝中形成特定肉芽肿为特征。免疫系统各脏器出现网状组织水肿和淋巴结增生。肠粘膜和黏膜下层表现性水肿,以及交替和坏死过程。
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引用次数: 0
Survey and Dynamics of bacteria involved in secondary infections in sheep affected by orf virus 羊口蹄疫病毒继发感染细菌的调查与动态
E. Pastor, P. Pojar, Crinu Mesteșanu, M. Spînu
In recent decades, secondary infection bacteria quickly increased their pathogenicity in both human and veterinary patients, due to their continuously increasing antibiotic-resistance. When such bacteria intervene in infections as orf in sheep, they can determine an important impact either in the sanitary or in the economic aspect of the brreding sector. Considering the relatively high spread and economic impact of orf in Romanian sheep, this research aimed to estimate the complexity of the secondary infections in diseased sheep in connection with the severity of the lesions displayed. The research was conducted in five herds, on 3 to 96 month old sheep, with orf lesions, scored from mild to severe. The bacteria were isolated from the lesions and identified by cultivation on broth and agar plates plus identification by use of API galleries. The severity of the lesions did not seem to be influenced by the number of bacterial species isolated, but by the herd environment and individual pathogenicity of the isolates.
近几十年来,由于抗生素耐药性不断增加,继发感染细菌在人类和兽医患者中的致病性迅速增加。当这些细菌干预绵羊的orf等感染时,它们可以决定对卫生或经济部门的重要影响。考虑到orf在罗马尼亚绵羊中的传播率和经济影响相对较高,本研究旨在评估患病绵羊继发感染的复杂性与病变的严重程度。这项研究在5个畜群中进行,对象是3至96个月大的绵羊,其orf病变从轻度到重度。从病变中分离出细菌,并通过在肉汤和琼脂平板上培养以及使用API培养基进行鉴定。病变的严重程度似乎不受分离菌株数量的影响,而是受分离菌株的群体环境和个体致病性的影响。
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引用次数: 0
Incidence and Public Health Risk Assessment of Toxic Metal Residues (cadmium and lead) in Liver and Kidney of Ovine and Bovine from Algeria 阿尔及利亚绵羊和牛肝脏和肾脏中有毒金属残留物(镉和铅)的发生率和公共卫生风险评估
Wahiba Zenad, Amel Benatallah, M. Zaouani, Sofiane Boudjellaba, L. Ainouz, Meriem Hind Ben Mahdi, A. Benouadah
This study intended to establish if Cd and Pb in the livers and kidneys of bovine and ovine slaughtered in the study area exceeded to concentrations maximum limit proposed by the European Commission (EC) 2006. After digestion of samples, Cadmium and lead concentrations were determined by Graphite Furnace Atomic Absorption Spectrophotometry (GFAAS). The results of our study have shown that lead and cadmium concentrations depend on age and species. The average fresh weight levels of liver and kidneys of bovine animals were about (0.319 mg/ kg and 0.337mg/kg) for Cd and (0,502mg/kg and 0.497mg kg) for Pb, while in ovine, mean concentrations in the liver and kidneys were (0,241mg/kg and 0.232 mg/kg) for Cd and (0.259 mg/kg and 0.265 mg/kg) for Pb. Concentrations above the maximum limit proposed by the EC 2006 for Pb and Cd have been detected in some liver and kidney samples from bovine older than 4 years and in ovine over 1 year old .Statistical analysis revealed a very significant (P<0.01) difference in Cd concentrations in the liver and kidneys between age groups and species.
本研究旨在确定在研究区域屠宰的牛和绵羊的肝脏和肾脏中的Cd和Pb是否超过了欧盟委员会(EC)2006年提出的最高浓度限值。样品消化后,用石墨炉原子吸收分光光度法测定镉和铅的浓度。我们的研究结果表明,铅和镉的浓度取决于年龄和物种。牛肝脏和肾脏的平均鲜重水平Cd约为(0.319 mg/kg和0.337 mg/kg),Pb约为(0502 mg/kg和0.497 mg/kg),而绵羊肝脏和肾脏中Cd的平均浓度分别为(0241 mg/kg和0.232 mg/kg)和(0.259 mg/kg和0.265 mg/kg)。在一些4岁以上的牛和1岁以上的绵羊的肝脏和肾脏样本中检测到铅和镉的浓度超过了EC 2006提出的最高限值。统计分析显示,不同年龄组和物种之间,肝脏和肾脏中的镉浓度存在非常显著的差异(P<0.01)。
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引用次数: 1
Research on the Values of Amplitudes of Electrocardiographic Waves Depending on Age in Kids 儿童心电图波幅值随年龄变化的研究
D. Cotor, G. Gâjâilă, A. Damian, A. Zagrai, C. Petcu, S. Călin, A. Raduta, Crina Andrei, G. Zagrai
The electrocardiogram (ECG) is a graphical recording of the cardiac electric activity during cardiac revolutions. This bio-current triggers and maintains the mechanical activity of the heart. Within this research, the amplitudes values of the electrocardiographic waves were determined in 6 leads: I, II, III, aVL, aVR and aVF. Thus, some electrocardiograms were recorded using limb lead in clinically healthy kids, aged 1 month, 3 months and 5 months, in order to achieve the proposed objectives. Then, the statistical analysis of the obtained results was performed using t (student) test. As a consequence of the interpretation of the obtained results, it was noticed that the limb leads can be used successfully for recording the electrocardiogram in kids because it provides an easy aspect to interpret. The highest amplitude of the electrocardiographic waves is recorded in I lead, in the case of the 1 month old kids (having the following values: 0.115 mV ± 0.010 for P wave; 0.625 mV ± 0.078 for QRS complex; 0.460 mV ± 0.045 for T wave) and in II lead (having the following values for the 3 months old kids: 0.071 mV ± 0.015 for P wave; 0.540 mV ± 0.064 for QRS complex; 0.310 mV ± 0.052 for T wave and having the following values for the 5 months old kids: 0.071 mV ± 0.015 for P wave; 0.455 mV ± 0.028 for QRS complex; 0.430 mV ± 0.026 for T wave). It also found that the lowest amplitude of electrocardiographic waves is recorded in the aVF lead, but this lead cannot be used for ECG recording in kids.
心电图(ECG)是心脏旋转期间心脏电活动的图形记录。这种生物电流触发并维持心脏的机械活动。在这项研究中,确定了6条导联的心电图波振幅值:I、II、III、aVL、aVR和aVF。因此,为了实现拟议的目标,在1个月、3个月和5个月的临床健康儿童中使用肢体铅记录了一些心电图。然后,使用t(student)检验对所获得的结果进行统计分析。由于对所获得的结果进行了解释,人们注意到肢体导联可以成功地用于记录儿童的心电图,因为它提供了一个易于解释的方面。心电图波形的最高振幅记录在I导联中,对于1个月大的儿童(具有以下值:P波0.115 mV±0.010;QRS波0.625 mV±0.078;T波0.460 mV±0.045)和II导联(对于3个月大的儿童,具有以下值:P波为0.071 mV±0.015;QRS波为0.540 mV±0.064;T波为0.310 mV±0.052;对于5个月大儿童,具有如下值:P波为0.071 mV±0.015,QRS波0.455 mV±0.028;T波0.430 mV±0.026)。研究还发现,aVF导联记录的心电图波振幅最低,但该导联不能用于儿童的心电图记录。
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引用次数: 0
Incidence and hormonal stimulation of cows with ovarian hypotrophy 奶牛卵巢功能减退的发生率及激素刺激
D. Berean, L. Bogdan, I. Bogdan, Anamaria Petrean, M. Cenariu, S. Bogdan
The purpose of this research was to establish the incidence and to compare the efficiency of two products used for the hormonal stimulation of ovarian hypotrophy. Gynaecological investigations were performed in 2 farms. The animals with ovarian hypotrophy (ovaries smaller than normal and with a slippery surface) were divided in 2 equal groups depending on the protocol applied: Progesterone (Prid Delta, CEVA) and Progesterone (Dehydroxyprogesterone 10%, Faculty of Veterinary Medicine Cluj-Napoca). In case of group 1 the treatment was performed with Prid Delta (CEVA), vaginal use, 1.55 g of progesterone/animal for 7 days. For the animals of group 2 the treatment was performed with Dehydroxyprogesterone, subcutaneous injection, 1.5 g of dehydroxyprogesterone/ animal for 7 days. For each group were observed: the estrous signals, the interval of estrous apparition, the number of gestation obtained after the first artificial insemination.
本研究的目的是确定用于卵巢功能减退激素刺激的两种产品的发生率并比较其效率。在2个农场进行妇科检查。卵巢萎缩(卵巢小于正常,表面光滑)的动物根据使用的方案分为两组:孕酮(Prid Delta, CEVA)和孕酮(去羟孕酮10%,克卢日纳波卡兽医学院)。第1组给予Prid Delta (CEVA),阴道使用,1.55 g孕酮/只,连续7天。2组大鼠给予去羟孕酮皮下注射,每只动物去羟孕酮1.5 g,连续7 d。观察各组受精卵的发情信号、发情出现的间隔时间、第一次人工授精后获得的妊娠次数。
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引用次数: 0
Antibiotic Resistance Profile in Co-Infecting Bacteria Isolated from Sheep Dipslaying Orf Lesions 绵羊双杀性Orf病变合并感染细菌的耐药性谱
E. Pastor, Crinu Mesteșanu, P. Pojar, M. Spînu
Antibiotic resistance in veterinary medicine poses lately increasing risks in therapeutic success and threatens the life of patients with various bacterial infections. Environmental bacteria are very likely to develop on the initial lesions induced by orf virus in sheep, worsening the development and prognosis of the disease. The research aimed at verifying the hypothesis that the persistence of the bacteria and thus, increased severity of the orf lesions is residing in the high antibiotic resistance of the co-infecting bacteria. The antibiotics used were gentamicine, cefuroxime, amoxicilline clavulanic acid, enrofloxacin, ciprofloxacin, penicillin and thrimetoprim. Multi antibiotic resistance indices were calculated for each strain. Based on the MAR index, at least two of the investigated environmental bacteria strains could further complicate orf lesions and delay the positive effects of the antibiotic therapy.
兽医中的抗生素耐药性最近在治疗成功方面带来了越来越大的风险,并威胁到各种细菌感染患者的生命。环境细菌很可能在绵羊orf病毒诱导的初始病变上发展,恶化疾病的发展和预后。这项研究旨在验证这样一种假设,即细菌的持久性以及orf病变严重程度的增加是由于共同感染细菌的高抗生素耐药性造成的。使用的抗生素有庆大霉素、头孢呋辛、阿莫西林-克拉维酸、恩诺沙星、环丙沙星、青霉素和三羟美他林。计算每个菌株的多种抗生素耐药性指数。根据MAR指数,至少有两种被调查的环境细菌菌株可能会使orf病变进一步复杂化,并延迟抗生素治疗的积极效果。
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引用次数: 0
Cryptosporidium Infection in Goats in Serbia 塞尔维亚山羊隐孢子虫感染
I. Pavlović, S. Ivanović, M. Petrović, V. Caro-Petrovic, D. Ružić-Muslić, J. Bojkovski, N. Zdravković
Cryptosporidiosis is an anthropozoonosis caused by coccidia of the family Cryptoporididae, whish may cause a serious health problem for kids in the first weeks of life. In our paper we presented results of examination of goat cryptosporidiosis in Serbia. In period 2016-2019 we examined 197 goat herds. Total of 1576 faecal smears were examined by Sheather’s sugar floatation technique and modified Ziehl–Neelsen staining technique. Cryptosporidiosis were found at 119 herds. Kids between five and twenty-one days old are the most susceptible. The morbidity varies from 75–100% and the mortality from 45-50%. Some animals do not develop into chronic cases and become carriers. After infection, animals either resist the organism, develop a mild infection that is self limiting, or soon sicken and die. Based on the performed research, we have established a significant role of cryptosporidiosis in the development of neonatal enteropathies of kids.
隐孢子虫病是一种由隐孢子虫科球虫引起的人畜共患病,可能在出生后的最初几周对儿童造成严重的健康问题。在我们的论文中,我们介绍了塞尔维亚山羊隐孢子虫病的检查结果。在2016-2019年期间,我们调查了197个山羊群。采用Sheather糖浮法和改良Ziehl-Neelsen染色法对1576例粪便涂片进行检测。119个猪群发现隐孢子虫病。5到21天大的孩子最容易受到影响。发病率为75-100%,死亡率为45-50%。有些动物不会发展成慢性病例而成为携带者。在感染后,动物要么抵抗这种有机体,发展为轻度感染并具有自限性,要么很快生病并死亡。根据所进行的研究,我们已经确定隐孢子虫病在儿童新生儿肠病发展中的重要作用。
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引用次数: 1
A Retrospective Study of Dogs with Osteomyelitis Secondary to Fractures (2016-2020) 犬骨折继发性骨髓炎的回顾性研究(2016-2020)
C. Igna, L. Schuszler, D. Bumb, R. Dascalu, C. Zaha, B. Sicoe
The purpose of this work was to evaluate the role of surgical procedures and the effectiveness of antimicrobial therapy in dogs with osteomyelitis secondary to fractures repair. Medical records between 2016 and 2020 were analyzed. Clinical data including signalment, affected bone segment, fracture type, isolated microorganisms, antimicrobial susceptibility profiles, applied therapeutic management and fracture healing time were documented. Eleven cases were confirmed with exogenous osteomyelitis of long bones secondary to fractures repair. The most common microorganisms isolated were Staphylococcus spp., Streptoccocus spp., and Escherichia coli. Amoxicillin and clavulanate potassium followed by ceftriaxone were the most effective drugs. High-resistance rates were documented for penicillin and clindamycin. All 10 cases undergoing surgical revision were cured in 10-17 weeks. Simultaneous debridement with implant removal, reduction, and rigid internal fracture fixation is a satisfactory method for revision surgery in treatment of long bones fractures complicated with osteomyelitis.
这项工作的目的是评估外科手术的作用和抗菌治疗在犬骨折修复继发骨髓炎中的有效性。分析了2016年至2020年的医疗记录。记录了临床数据,包括信号、受影响的骨段、骨折类型、分离的微生物、抗菌药物敏感性、应用的治疗管理和骨折愈合时间。11例被证实为继发于骨折修复的外源性长骨骨髓炎。分离出的最常见的微生物是葡萄球菌属、链霉菌属和大肠杆菌。阿莫西林和克拉维酸钾是最有效的药物,其次是头孢曲松。青霉素和克林霉素的耐药率较高。10例患者均在10~17周内治愈。同时清创、移植物、复位和刚性骨折内固定是治疗长骨骨折合并骨髓炎的一种令人满意的翻修手术方法。
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引用次数: 0
Dental Treatment for a European Red Tail Deer 一只欧洲红尾鹿的牙齿治疗
Raluca Ioana Nedelea, A. Toma, Andreea Mărincuș
The veterinarian doctor has to be prepared to help various kind of species. Our team had to perform dental treatment for a five years old European red tail deer. The deer was eating with difficulty. Even thou it was a hot summertime, the hind presented late shedding. Her calm and kind behaviour changed into an aggressive one. She was biting cold, metal objects, obviously to calm her dental pain. Our approach aimed to re-establish the biological and physiological balance of the oral cavity. A thorough general exam was performed for differential diagnosis. Oral examination revealed an aggressive periodontal disease. Surgical extractions were the only possible treatment. One year follow up is good, changing the prognosis from a reserved one into very good.
兽医必须准备好帮助不同种类的动物。我们的团队必须为一只五岁的欧洲红尾鹿进行牙齿治疗。鹿吃东西很吃力。尽管这是一个炎热的夏天,母鹿还是很晚才掉毛。她平静和善的行为变成了好斗的行为。她咬着冰冷的金属物品,显然是为了缓解牙痛。我们的方法旨在重建口腔的生物和生理平衡。进行了彻底的全身检查以进行鉴别诊断。口腔检查显示有侵袭性牙周病。手术摘除是唯一可行的治疗方法。1年随访良好,预后由保留变为非常好。
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引用次数: 0
The Branching Pattern of the Aortic Arch in Gallus Domesticus 家鸡主动脉弓的分支模式
I. Roman, C. Martonoș, C. Dezdrobitu, Alina Berlea, Iulia Preja, A. Irimie, Raluca Flocea, Ioana Chirlean, A. Gudea, F. Stan, A. Damian
In birds, in contrast to mammals, two brachiocephalic trunks are the origin in the arch of aorta and give rise to the subclavian arteries and common carotid. The aim of this study was to investigate the vascular branching morphology of the aortic arch in Gallus Domesticus with the purpose of providing accurate data with regards to the arterial supply of these segments to researchers and clinicians. The biological material was represented by 10 adult chicken bodies with an average weight of 2 kg, females. After procurement of the biological material, the feathers, skin, muscles and sternum were removed for easy access to the heart. After left ventricular cannulation, epoxy resin mixed with red dye was injected. The body was immersed in 5% formaldehyde for 24 hours. Next, stratigraphic dissection was performed. Two brachiocephalic trunks branched continually from aortic arch caudoventral to the primary bronchi. The left subclavian artery gave rise to sternoclavicular, thoracic, axillary and intercostal and the same was observed to the right side. Due to this method we could observe the aortic arch of Gallus Domesticus being different from mammals in that the left and right brachiocephalic trunks are detached from it.
在鸟类中,与哺乳动物不同,两条头臂干起源于主动脉弓,并产生锁骨下动脉和颈总动脉。本研究的目的是研究Gallus Domesticus主动脉弓的血管分支形态,目的是为研究人员和临床医生提供有关这些节段动脉供应的准确数据。生物材料由10只平均重量为2kg的成年雌性鸡体表示。在获得生物材料后,去除羽毛、皮肤、肌肉和胸骨,以方便进入心脏。左心室插管后,注射掺有红色染料的环氧树脂。将尸体浸泡在5%甲醛中24小时。接下来,进行了地层解剖。两个臂头干从主动脉弓尾中央连续分支到主支气管。左锁骨下动脉产生胸锁、胸、腋和肋间,右侧也有同样的情况。由于这种方法,我们可以观察到Gallus Domesticus的主动脉弓与哺乳动物的不同之处在于左右臂头干与主动脉弓分离。
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引用次数: 0
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Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Horticulture
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