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Air DNA forensics: Novel air collection method investigations for human DNA identification. 空气 DNA 鉴证:用于人类 DNA 鉴定的新型空气采集法调查。
Pub Date : 2024-11-10 DOI: 10.1111/1556-4029.15662
Emily Bibbo, Duncan Taylor, Roland A H van Oorschot, Mariya Goray

Modern techniques can generate highly discriminatory DNA profiles from minuscule biological samples, providing valuable information in criminal investigations and court proceedings. However, trace and touch DNA samples, due to their nature, often have lower success rates than other biological materials, such as blood. Further, forensically aware criminals can utilize gloves and meticulously clean the crime scene to remove DNA traces of themselves from contacted surfaces. Air sampling offers a novel approach to the collection of human DNA that has the potential to bypass some of these issues. This study reports on the results of research into the prevalence and persistence of human DNA in the air. The ability to collect human DNA from the air was investigated with the use of an AirPrep Cub Sampler ACD220 in different spaces, with and without the presence of individuals for various durations of sample collection. Results of this study demonstrate that level of occupation and sampling duration each have an influence on quantity and quality of DNA recovered from the air whereas the effects of orientation and distance of participants from the collection device as well as sequence of occupation remain unclear and require further investigation.

现代技术可以从微小的生物样本中生成具有高度鉴别性的 DNA 图谱,为刑事调查和法庭诉讼提供有价值的信息。然而,与血液等其他生物材料相比,痕量和接触 DNA 样本因其特性,成功率往往较低。此外,具有法医意识的罪犯可以使用手套并仔细清洁犯罪现场,以清除接触表面上的 DNA 痕迹。空气取样为人类 DNA 的采集提供了一种新方法,有可能绕过上述一些问题。这项研究报告了对空气中人类 DNA 的普遍性和持久性的研究结果。研究人员使用 AirPrep Cub 采样器 ACD220,在不同的空间、有人员在场和没有人员在场的情况下,以不同的样本采集时间,对从空气中采集人类 DNA 的能力进行了调查。这项研究的结果表明,占用水平和采样持续时间对从空气中采集到的 DNA 的数量和质量都有影响,而参与者与采集装置的方向和距离以及占用顺序的影响仍不清楚,需要进一步研究。
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引用次数: 0
No practice effect on the classification accuracy of the response time concealed information test. 练习对反应时间隐藏信息测试的分类准确性没有影响。
Pub Date : 2024-11-08 DOI: 10.1111/1556-4029.15656
Gáspár Lukács, Izumi Matsuda

The Response Time Concealed Information Test can reveal that a person recognizes a relevant item (probe, e.g., a murder weapon) among other, irrelevant items (controls), based on slower responses to the probe compared to the controls. A previous study (Lukács, 2022, JARMAC) analyzed the data of 14 experiments and demonstrated that classification accuracy is increased by increased test length (i.e., increased number of trials included in the analysis). However, that study left the important question open whether prior practice (whose trials are not included in the analysis) influences the classification accuracy of subsequent testing (i.e., subsequent trials included in the analysis). Reanalyzing the same data from the 14 experiments (comprising 2223 individual tests), we show that different sections of the test (each with the same number of trials), such as the first and second half of each examined test, do not differ substantially in their classification accuracy. The main implications for real-life application are that, at least up to about 600 trials, prior practice does not affect subsequent tests' results, and the number of examined relevant items or their order of presentation may be freely chosen without compromising the method's validity.

反应时间隐藏信息测试可以揭示出,一个人在其他无关项目(对照组)中识别出一个相关项目(探针,例如凶器),其依据是对探针的反应比对照组慢。之前的一项研究(Lukács,2022 年,JARMAC)分析了 14 个实验的数据,证明分类准确率会随着测试长度的增加(即分析中包含的试验次数增加)而提高。然而,该研究留下了一个重要问题,即先前的练习(其试验未纳入分析)是否会影响后续测试(即纳入分析的后续试验)的分类准确性。通过重新分析来自 14 次测试(包括 2223 次测试)的相同数据,我们发现测试的不同部分(每个部分的测试次数相同),如每次测试的前半部分和后半部分,在分类准确性上并无实质性差异。这对实际应用的主要意义在于,至少在大约 600 次测试之前,先前的练习不会影响后续测试的结果,而且可以自由选择相关测试项目的数量或呈现顺序,而不会影响该方法的有效性。
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引用次数: 0
Evaluation of interferents in sampling materials for analysis of post-explosion residues (explosive emulsion/ANFO) using gas chromatography-mass spectrometry (GC/MS). 评估使用气相色谱-质谱法(GC/MS)分析爆炸后残留物(爆炸乳剂/ANFO)的取样材料中的干扰物。
Pub Date : 2024-11-08 DOI: 10.1111/1556-4029.15657
Lúcio Paulo Lima Logrado, Jez Willian Batista Braga

Chemical analysis aimed at identifying post-explosion residues is critical for investigating crimes or accidents involving explosives, establishing it as a vital area of forensic chemistry. In general, only trace amounts remain in samples analyzed for this purpose, making the presence of interferents a constant concern. Therefore, understanding the materials used from collection to sample preparation is essential for this type of analysis. This study focuses on organic contaminants in collection and laboratory materials analyzed by gas chromatography/mass spectrometry (GC/MS), complementing a previous study by Mauricio et al. (2020), which addressed inorganic contaminants in materials used for similar purposes in ion chromatography (IC). No prior studies have specifically investigated the potential organic interferences introduced by various materials commonly used in forensic laboratories for sample pre-processing, storage, and evidence collection in this context. Plastic films, bottle caps, disposable gloves, syringes, swabs, disposable cups, plastic tubes, and plastic pipettes were examined. GC/MS analyses revealed that certain materials, particularly syringe plungers, gloves, and plastic films, can interfere with the detection of post-explosion residues from explosive emulsions and ANFO (ammonium nitrate-fuel oil). These findings emphasize the importance of evaluating materials for potential interferences prior to sample collection and processing to minimize contamination risks. This study provides significant insights into how commonly used laboratory materials can impact forensic analysis, thereby enhancing the reliability of post-explosion residue analysis and supporting more accurate forensic investigations.

旨在确定爆炸后残留物的化学分析对于调查涉及爆炸物的犯罪或事故至关重要,因此成为法医化学的一个重要领域。一般来说,为此目的进行分析的样本中只残留痕量物质,因此干扰物的存在始终是一个令人担忧的问题。因此,了解从采集到样品制备所使用的材料对此类分析至关重要。本研究的重点是通过气相色谱/质谱法(GC/MS)分析采集和实验室材料中的有机污染物,是对 Mauricio 等人(2020 年)之前研究的补充,后者研究了离子色谱法(IC)中用于类似目的的材料中的无机污染物。此前没有研究专门调查过法医实验室在样本预处理、储存和证据收集过程中常用的各种材料可能带来的有机干扰。研究人员检查了塑料薄膜、瓶盖、一次性手套、注射器、棉签、一次性杯子、塑料管和塑料吸管。GC/MS 分析表明,某些材料,尤其是注射器活塞、手套和塑料薄膜,会干扰爆炸物乳化液和 ANFO(硝酸铵-燃料油)爆炸后残留物的检测。这些发现强调了在样本采集和处理之前对材料进行潜在干扰评估以最大程度降低污染风险的重要性。这项研究就实验室常用材料如何影响法医分析提供了重要见解,从而提高了爆炸后残留物分析的可靠性,支持了更准确的法医调查。
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引用次数: 0
Antibiotic beads found with a skeletal example of complications from a hip replacement. 在髋关节置换术并发症的骨骼实例中发现抗生素珠。
Pub Date : 2024-11-08 DOI: 10.1111/1556-4029.15661
Alexia Alexander, Alexander J Smith, Melissa A Connor

During maceration, a string of beads was found near the surgically modified right os coxa and femur of the remains of a 55-year-old woman donated to the Forensic Investigation Research Station at Colorado Mesa University. The remains were initially used in the study of decomposition of human remains, and then macerated to clean the skeleton. Documentation on this decedent noted three separate "hip replacement" surgeries, but no other details. The surgical modifications include significant alterations to the right os coxa and femur consistent with a Girdlestone procedure, generally used to treat an infected hip replacement unresponsive to other treatment. In this procedure, the surgeon removes the femoral head, and in this case, also the portion of the replacement in the os coxa. In this individual, a portion of the proximal femur was reflected, possibly to reduce contact with the os coxa. The beads are consistent with antibiotic beads, used to treat the infection that likely necessitated the Girdlestone procedure. Both the operation and the inclusion of the antibiotic beads are unusual and are potentially useful in the identification process.

在浸渍过程中,在一名捐赠给科罗拉多梅萨大学法医调查研究站的 55 岁女性遗骸经手术改造的右胫骨和股骨附近发现了一串珠子。这具遗骸最初被用于研究人类遗骸的腐烂情况,随后被浸渍以清洁骨骼。有关该死者的文件记录了三次单独的 "髋关节置换 "手术,但没有其他细节。手术改动包括对右髋臼和股骨的重大改动,与 Girdlestone 手术一致,该手术通常用于治疗对其他治疗无效的感染性髋关节置换术。在这种手术中,外科医生会切除股骨头,在这个病例中还会切除髋臼中的置换部分。在这个病例中,股骨近端部分被反射,可能是为了减少与股骨颈的接触。这些珠子与抗生素珠子一致,都是用来治疗感染的,而感染很可能是进行 Girdlestone 手术的必要条件。该手术和抗生素珠的出现都很不寻常,可能有助于鉴定过程。
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引用次数: 0
Mercy killing of a 72-year-old woman through heroin intoxication. 因海洛因中毒而怜悯杀害一名 72 岁的妇女。
Pub Date : 2024-11-07 DOI: 10.1111/1556-4029.15637
Johann Zwirner, Stefanie Iwersen-Bergmann, Klaus Püschel, Benjamin Ondruschka

Active euthanasia is legally permissible in only eight jurisdictions worldwide and may only be administered by qualified personnel following specific selection criteria. Mercy killing refers to the deliberate termination of the life of an individual suffering from a terminal chronic medical condition. Detecting both illegally performed active euthanasia and instances of mercy killing presents challenges for forensic pathologists. The presented case describes a mercy killing involving a 72-year-old woman with multiple chronic conditions who was killed by her grandson via heroin intoxication after administration of the anxiolytic alprazolam. Key findings from the external postmortem examination included a single fresh injection site on the inside of the elbow and a superficial T-shaped cut on the flexor side of the left forearm. Toxicological analyses revealed elevated blood levels of heroin metabolites, including 6-monoacetylmorphine and absence of hydroxyalprazolam, indicating an only short survival time following heroin injection. A cocaine concentration in blood was comparatively low but rather high in hair samples. Elderly individuals with multiple chronic conditions are at increased risk of becoming homicide victims. Comprehensive forensic documentation of injection sites is essential to avoid overlooking deaths caused by injection and to differentiate them from medical measures during resuscitation attempts.

全世界只有八个司法管辖区在法律上允许主动安乐死,而且只能由合格人员按照特定的选择标准实施。仁慈杀害是指蓄意终止患有晚期慢性疾病的人的生命。检测非法实施的主动安乐死和怜悯杀人事件对法医病理学家来说都是一项挑战。本病例描述了一起涉及一名患有多种慢性疾病的 72 岁妇女的怜悯杀人案,她在服用抗焦虑药阿普唑仑后被其孙子以海洛因中毒的方式杀害。尸检的主要发现包括:肘部内侧有一处新鲜注射部位,左前臂屈侧有一处浅表 T 形切口。毒理学分析表明,血液中的海洛因代谢物(包括 6-monoacetylmorphine)含量升高,但没有羟丙唑仑,这表明注射海洛因后存活时间很短。血液中的可卡因浓度相对较低,但头发样本中的可卡因浓度却相当高。患有多种慢性疾病的老年人成为凶杀案受害者的风险更高。对注射部位进行全面的法医记录对于避免忽视注射导致的死亡以及将其与复苏尝试中的医疗措施区分开来至关重要。
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引用次数: 0
Beyond binary: Analyzing closed-source data to compare specific roles and behaviors within violent and nonviolent terrorist involvement. 超越二元对立:分析闭源数据,比较暴力和非暴力恐怖主义参与中的特定角色和行为。
Pub Date : 2024-11-07 DOI: 10.1111/1556-4029.15648
Amber Seaward, Zoe Marchment, Caitlin Clemmow, Frank Farnham, Richard Taylor, Luc Taperell, Sara Henley, Sara Boulter, Karen Townend, Paul Gill

Increasingly, studies compare risk and protective factors for involvement in violent and nonviolent terrorist behaviors. This exploratory study investigates whether this distinction is sufficient, or whether it should be disaggregated further into more granular terrorist roles and behaviors. Using data on 404 referrals to a UK countering violent extremism Prevent hub specializing in mental health and associated needs, we compare violent and nonviolent referrals, and then more specific behaviors (vulnerability, proactive extremism, foreign fighting, and violence planning). Bivariate and multivariate analyses show there is value in disaggregating beyond the binary violence versus nonviolence distinction, as more (and more detailed) relationships emerged when using the disaggregated set of behaviors. While gender did not differentiate violent and nonviolent referrals, women were more likely to be referred for radicalization vulnerability or potential foreign fighting. Extreme right-wing and extreme Islamist referrals were no more or less violent overall, but Islamist referrals were disproportionately referred for both the most and least violent behaviors. Personality and developmental disorders were associated with violence, and disaggregated behaviors provided detailed insight into the drivers of these associations. These exploratory findings, while interesting, likely do not generalize beyond our specific sample. Instead, this study's value lies in demonstrating the utility for both research and, eventually, practice of disaggregating beyond violence and nonviolence. The results demonstrate clear operational implications for threat assessment in the need to include a more refined set of risk factors to aid in assessing risk of more relevant outcomes than terrorist involvement overall.

越来越多的研究对参与暴力和非暴力恐怖主义行为的风险和保护因素进行了比较。这项探索性研究探讨了这种区分是否足够,或者是否应进一步细分为更细化的恐怖主义角色和行为。我们利用英国打击暴力极端主义预防中心的 404 个转介数据,比较了暴力和非暴力转介,以及更具体的行为(脆弱性、主动极端主义、对外战斗和暴力计划)。双变量和多变量分析表明,除了暴力与非暴力的二元区分外,还有其他分类价值,因为在使用分类行为集时,会出现更多(更详细)的关系。虽然性别并没有区分暴力和非暴力的转介,但女性更有可能因激进化脆弱性或潜在的对外战斗而被转介。极端右翼和极端伊斯兰教转介人员的暴力程度总体上并无高低之分,但伊斯兰教转介人员因最严重和最轻微的暴力行为而被转介的比例过高。人格障碍和发育障碍与暴力有关,而分类行为则提供了对这些关联驱动因素的详细了解。这些探索性发现虽然很有趣,但很可能不会超出我们特定样本的范围。相反,本研究的价值在于证明了对暴力和非暴力行为进行分类对研究和最终实践的实用性。研究结果对威胁评估具有明确的操作意义,即需要纳入一套更精细的风险因素,以帮助评估比参与恐怖活动更相关的风险。
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引用次数: 0
The use of dietary isotopes as a preliminary step in the death investigation of unidentified skeletal human remains in British Columbia, Canada. 在加拿大不列颠哥伦比亚省对身份不明的人类遗骸进行死亡调查时,使用膳食同位素作为初步步骤。
Pub Date : 2024-11-07 DOI: 10.1111/1556-4029.15653
Damon Tarrant, Laura Yazedjian, Joe Hepburn, Stephen Fonseca, Sahra Talamo, Michael Richards

In British Columbia, Canada, unidentified skeletal human remains are often recovered by law enforcement or civilians and there is a question if they are modern and of medicolegal significance, or historical or archaeological. We used relatively fast and inexpensive carbon and nitrogen stable isotope analysis of human bone collagen from a selection of these remains (n = 48) combined with a logistic regression model to classify remains as modern, historical, or archaeological. We then confirmed our temporal classification through directly radiocarbon dating each individual and found that we could predict the temporal group with 93% accuracy. In regions where archaeological remains have dietary isotope values distinct from living people, dietary stable isotope analysis can provide a time-, and resource-efficient method to screen cases of unidentified human remains early in death investigation.

在加拿大不列颠哥伦比亚省,执法人员或平民经常会发现一些身份不明的人类遗骸,人们不禁要问,这些遗骸是现代的、具有医学法律意义的遗骸,还是历史的或考古的遗骸。我们使用相对快速和廉价的碳和氮稳定同位素分析方法,对这些遗骸中的一部分(n = 48)的人类骨骼胶原蛋白进行分析,并结合逻辑回归模型将遗骸分为现代遗骸、历史遗骸或考古遗骸。然后,我们通过直接对每个个体进行放射性碳测年,确认了我们的时间分类,并发现我们预测时间组别的准确率高达 93%。在考古遗骸的膳食同位素值与活人不同的地区,膳食稳定同位素分析可以提供一种省时、省资源的方法,在死亡调查早期筛选身份不明的人类遗骸。
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引用次数: 0
GAMEPLANS: A template for robust digital evidence strategy development. GAMEPLANS:强有力的数字证据战略开发模板。
Pub Date : 2024-11-07 DOI: 10.1111/1556-4029.15655
Graeme Horsman

Law enforcement officers should now expect to encounter forms of digital evidence at most of their inquiries, and as a result ensure they are prepared to effectively deal with it. This should involve the production of a digital evidence strategy (DES) which describes those actions required of any investigative team to effectively identify, collect, examine, and evaluate any digital devices/data, while also defining the circumstances for when it is appropriate to conduct such tasks. To help officers to produce robust DESs this work provides a DES template which utilizes the "GAMEPLANS" acrostic to identify nine fundamental components that are required of all DESs-"G"-Grounds for investigation; "A"-Authorization; "M"-Method of investigation; "E"-Evaluation of the meaning of any findings; "P"-Proportionality; "L"-Logic; "A"-Agreement; "N"-Necessity; "S"-Scrutiny. Each of these components are described including the sub-tasks that are contained within each, which any officer constructing a robust and effective DES must address (and provide evidence of having addressed). To support this, a DES template file is also provided, which can be utilized by officers.

现在,执法人员在大多数调查中都会遇到各种形式的数字证据,因此,他们应确保做好准备,有效地处理这些数字证据。这应涉及数字证据策略(DES)的制作,该策略描述了任何调查小组为有效识别、收集、检查和评估任何数字设备/数据而需要采取的行动,同时还定义了适合执行此类任务的情况。为帮助官员编写可靠的 "调查数据报告",这项工作提供了一个 "调查数据报告 "模板,该模板利用 "GAMEPLANS "缩略语确定了所有 "调查数据报告 "所需的九个基本组成部分--"G"--调查依据;"A"--授权;"M"--调查方法;"E"--对任何调查结果意义的评估;"P"--相称性;"L"--逻辑;"A"--协议;"N"--必要性;"S"--审查。描述了这些组成部分中的每一部分,包括每一部分所包含的子任务,任何官员在构建稳 健有效的 DES 时都必须解决这些问题(并提供解决这些问题的证据)。为支持这一点,还提供了一个 DES 模板文件,供官员使用。
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引用次数: 0
Acid attacks on pin-tumbler locks. 用酸性物质攻击针刺锁。
Pub Date : 2024-11-05 DOI: 10.1111/1556-4029.15647
Lars Oliver Meisel, Matthias Weber

Recently, an unusual method of home burglary has emerged in several European countries. Nitric acid is used to attack the tumbler lock of the front door. The aim of this study is to gain insights into the technical functioning of this method and to evaluate the advantages and disadvantages of this modus operandi. For this purpose, four pin-tumbler locks were attached to a custom-made holder and 65% nitric acid was successively injected into the key channel using a syringe. The locks were checked manually and using a CT scanner to see whether the lock was overcome and how the acid did affect the pins and springs. In three out of four cases the lock was overcome. The shortest time was 22 min until the pin pairs could be removed and it was possible to turn the core with a screwdriver. Overall, the results show that the modus operandi works with standard locks. According to the authors, the long duration and the high health risk are significant disadvantages in relation to other overcoming methods.

最近,欧洲一些国家出现了一种不同寻常的入室盗窃方法。这种方法是用硝酸来破坏前门的活锁。本研究的目的是深入了解这种方法的技术运作,并评估这种作案手法的优缺点。为此,我们将四把插销式不倒翁锁固定在一个特制的支架上,然后用注射器将 65% 的硝酸连续注入钥匙通道。通过手动和 CT 扫描仪对锁进行检查,以确定锁是否被破坏,以及酸液对插销和弹簧的影响。在四个案例中,有三个案例的锁都被打开了。最短的一次用了 22 分钟,直到销钉对可以移除,并且可以用螺丝刀转动锁芯。总之,结果表明,该操作方法适用于标准锁。作者认为,与其他克服方法相比,持续时间长和健康风险高是明显的缺点。
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引用次数: 0
Letter to the editor-The transition point from zero-order to first order in blood alcohol elimination curves. Where is it? 致编辑的信-血液酒精消除曲线从零阶到一阶的过渡点。它在哪里?
Pub Date : 2024-11-04 DOI: 10.1111/1556-4029.15650
Peter D Maskell, Alanna de Korompay
{"title":"Letter to the editor-The transition point from zero-order to first order in blood alcohol elimination curves. Where is it?","authors":"Peter D Maskell, Alanna de Korompay","doi":"10.1111/1556-4029.15650","DOIUrl":"https://doi.org/10.1111/1556-4029.15650","url":null,"abstract":"","PeriodicalId":94080,"journal":{"name":"Journal of forensic sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142577108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Journal of forensic sciences
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