Rodent scavenging of human remains is repeatedly documented in literature. However, most of this documentation is based on single-case examples. While this information is valuable, it does not provide an in-depth understanding of scavenger behavior in a single environment. Mouse scavenging data, in particular, lack larger sample sizes and data representing the full duration of scavenging activity. In documented cases, mice scavenge primarily the hands, the feet, and the head without significant progression into the rest of the body. At the Forensic Investigation Research Station in Whitewater, Colorado, deer mice (Peromyscus maniculatus group) scavenged tissue from 11 human donors. Motion activated game cameras and regular field photography documented scavenging progression and deer mouse behavior. Deer mice generally showed a preference for skin and fat, rarely consuming enough muscle to expose bone. The most scavenged sections of the body were the limbs and deer mice generally ignored the hands, the feet, and the head, unlike previous documentation. In a few cases, deer mice consumed tissue on the limbs in a distinct trail pattern. Most of the scavenging began in spring. This sample greatly expands on the existing documentation of mice overall and contributes much needed data on the progression of scavenging. Scavengers can have a significant impact on a death scene, so detailed documentation of different scavengers in a variety of environments can prove useful in the investigation process.
{"title":"Soft tissue scavenging patterns of mice on human remains.","authors":"Alexander J Smith","doi":"10.1111/1556-4029.15649","DOIUrl":"https://doi.org/10.1111/1556-4029.15649","url":null,"abstract":"<p><p>Rodent scavenging of human remains is repeatedly documented in literature. However, most of this documentation is based on single-case examples. While this information is valuable, it does not provide an in-depth understanding of scavenger behavior in a single environment. Mouse scavenging data, in particular, lack larger sample sizes and data representing the full duration of scavenging activity. In documented cases, mice scavenge primarily the hands, the feet, and the head without significant progression into the rest of the body. At the Forensic Investigation Research Station in Whitewater, Colorado, deer mice (Peromyscus maniculatus group) scavenged tissue from 11 human donors. Motion activated game cameras and regular field photography documented scavenging progression and deer mouse behavior. Deer mice generally showed a preference for skin and fat, rarely consuming enough muscle to expose bone. The most scavenged sections of the body were the limbs and deer mice generally ignored the hands, the feet, and the head, unlike previous documentation. In a few cases, deer mice consumed tissue on the limbs in a distinct trail pattern. Most of the scavenging began in spring. This sample greatly expands on the existing documentation of mice overall and contributes much needed data on the progression of scavenging. Scavengers can have a significant impact on a death scene, so detailed documentation of different scavengers in a variety of environments can prove useful in the investigation process.</p>","PeriodicalId":94080,"journal":{"name":"Journal of forensic sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142570867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In forensic handwriting analysis, it is crucial to understand the relative frequencies of findings relevant to the specific author, especially when using statistical methods. These are factored into the likelihoods used to determine the probabilities for the different authorship hypotheses. However, if ad hoc writings are included in the comparison materials, the representation of a comparison writer's habits can be distorted. An overrepresentation of certain creation time points can be avoided by treating ad hoc series of comparison writing samples as internally homogeneous agglomerates, incorporating only a single value per series into the average relative frequency of a given finding for a comparison writer. Additionally, the proposed approach produces finding counts largely independent of the length of the handwriting sample, which has a positive impact on the efficiency and cost-effectiveness of the expert evaluation.
{"title":"Handling finding counts in handwriting analysis - Avoiding the overrepresentation of unusual writing scenarios.","authors":"Rolf Berty","doi":"10.1111/1556-4029.15643","DOIUrl":"https://doi.org/10.1111/1556-4029.15643","url":null,"abstract":"<p><p>In forensic handwriting analysis, it is crucial to understand the relative frequencies of findings relevant to the specific author, especially when using statistical methods. These are factored into the likelihoods used to determine the probabilities for the different authorship hypotheses. However, if ad hoc writings are included in the comparison materials, the representation of a comparison writer's habits can be distorted. An overrepresentation of certain creation time points can be avoided by treating ad hoc series of comparison writing samples as internally homogeneous agglomerates, incorporating only a single value per series into the average relative frequency of a given finding for a comparison writer. Additionally, the proposed approach produces finding counts largely independent of the length of the handwriting sample, which has a positive impact on the efficiency and cost-effectiveness of the expert evaluation.</p>","PeriodicalId":94080,"journal":{"name":"Journal of forensic sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142549762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Animal-related crimes have increased with an increase in the number of pets worldwide, underscoring the importance of animal-related biological evidence at crime scenes. Evidence obtained in cases involving dogs and cats often includes a mixture of human and animal DNA. In this study, we developed a method using droplet digital polymerase chain reaction (ddPCR) to simultaneously identify and quantitatively detect human, dog, and cat DNA in mixed samples. HLA-DRA was chosen as a human-specific marker, OR6D7 as a dog-specific marker, and FLAI-K as a cat-specific marker. The species specificity of each target was confirmed using 14 control DNA samples from 11 mammals and 3 birds. Sensitivity tests determined the limit of detection to be 0.0008 ng/μL for human DNA and 0.00061 ng/μL for dog and cat DNA. In the mixture test, each DNA sample was independently and accurately detected in samples containing trace amounts of all three types of DNA. This study demonstrated the effectiveness of applying ddPCR to forensic case samples from dog- and cat-related incidents. We have presented a reliable method for the accurate identification and quantification of human, dog, and cat DNA simultaneously, offering possibilities for advancements in forensic DNA analysis and related fields.
随着全球宠物数量的增加,与动物有关的犯罪也在增加,这凸显了犯罪现场与动物有关的生物证据的重要性。在涉及猫狗的案件中获得的证据通常包括人类和动物 DNA 的混合物。在这项研究中,我们开发了一种使用液滴数字聚合酶链反应(ddPCR)的方法,可同时鉴定和定量检测混合样本中的人、狗和猫的 DNA。我们选择 HLA-DRA 作为人的特异性标记,OR6D7 作为狗的特异性标记,FLAI-K 作为猫的特异性标记。使用来自 11 种哺乳动物和 3 种鸟类的 14 个对照 DNA 样本确认了每个目标的物种特异性。灵敏度测试表明,人类 DNA 的检测限为 0.0008 ng/μL,狗和猫 DNA 的检测限为 0.00061 ng/μL。在混合物测试中,每种 DNA 样品都能在含有微量三种 DNA 的样品中被独立、准确地检测出来。这项研究证明了将 ddPCR 应用于与狗和猫有关的法医案件样本的有效性。我们提出了一种同时准确鉴定和量化人、狗和猫 DNA 的可靠方法,为法医 DNA 分析及相关领域的发展提供了可能。
{"title":"Beyond the mix: Advancements in simultaneous detection and quantification of human, dog, and cat DNA.","authors":"Hyeon-Jin Bae, Kyu-Sik Jeong, Jung-Eun Kim, Eun-Mi Hwang, Seong Yeon Yoo","doi":"10.1111/1556-4029.15644","DOIUrl":"https://doi.org/10.1111/1556-4029.15644","url":null,"abstract":"<p><p>Animal-related crimes have increased with an increase in the number of pets worldwide, underscoring the importance of animal-related biological evidence at crime scenes. Evidence obtained in cases involving dogs and cats often includes a mixture of human and animal DNA. In this study, we developed a method using droplet digital polymerase chain reaction (ddPCR) to simultaneously identify and quantitatively detect human, dog, and cat DNA in mixed samples. HLA-DRA was chosen as a human-specific marker, OR6D7 as a dog-specific marker, and FLAI-K as a cat-specific marker. The species specificity of each target was confirmed using 14 control DNA samples from 11 mammals and 3 birds. Sensitivity tests determined the limit of detection to be 0.0008 ng/μL for human DNA and 0.00061 ng/μL for dog and cat DNA. In the mixture test, each DNA sample was independently and accurately detected in samples containing trace amounts of all three types of DNA. This study demonstrated the effectiveness of applying ddPCR to forensic case samples from dog- and cat-related incidents. We have presented a reliable method for the accurate identification and quantification of human, dog, and cat DNA simultaneously, offering possibilities for advancements in forensic DNA analysis and related fields.</p>","PeriodicalId":94080,"journal":{"name":"Journal of forensic sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142549761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Forensic firearms and tool mark examiners compare bullets and cartridge cases to assess whether they originate from the same source or different sources. To communicate their observations, they rely on predefined conclusion scales ranging from Identification to Elimination. However, these terms have not been calibrated against the actual strength of the evidence except indirectly through error rate studies. The present research reanalyzes the findings of firearms and cartridge case comparisons from error rate studies to generate a quantitative measure of the strength of the evidence for each comparison. We use an ordered probit model to summarize the distribution of responses of examiners and aggregate the data for all comparisons to produce a set of likelihood ratios. The likelihood ratios can be as low as less than 10, which does not seem to justify the current articulation scale that may imply a strength of evidence of 10,000 or greater. This suggests that examiners are using language that overstates the strength of the evidence by several orders of magnitude.
{"title":"Quantifying the strength of firearms comparisons based on error rate studies.","authors":"Nada Aggadi, Kimberley Zeller, Tom Busey","doi":"10.1111/1556-4029.15646","DOIUrl":"https://doi.org/10.1111/1556-4029.15646","url":null,"abstract":"<p><p>Forensic firearms and tool mark examiners compare bullets and cartridge cases to assess whether they originate from the same source or different sources. To communicate their observations, they rely on predefined conclusion scales ranging from Identification to Elimination. However, these terms have not been calibrated against the actual strength of the evidence except indirectly through error rate studies. The present research reanalyzes the findings of firearms and cartridge case comparisons from error rate studies to generate a quantitative measure of the strength of the evidence for each comparison. We use an ordered probit model to summarize the distribution of responses of examiners and aggregate the data for all comparisons to produce a set of likelihood ratios. The likelihood ratios can be as low as less than 10, which does not seem to justify the current articulation scale that may imply a strength of evidence of 10,000 or greater. This suggests that examiners are using language that overstates the strength of the evidence by several orders of magnitude.</p>","PeriodicalId":94080,"journal":{"name":"Journal of forensic sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142549763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sex estimation is a critical component of the biological profile, and forensic anthropologists may use a variety of sex estimation methods depending upon the degree of completeness and state of preservation of the skeletal remains being analyzed. The innominate is widely accepted to be the most sexually dimorphic skeletal element. The Diagnose Sexuelle Probabiliste (DSP) method, which uses 10 measurements of the innominate, was introduced in 2005 and updated as DSP2 in 2017. While DSP2 has been reported to have high classification accuracy rates in studies of South American and European populations, the method has not been widely tested in US samples, and few US practitioners incorporate this method into their casework. The goal of this study was to test the reliability and accuracy of DSP2 using a large, modern sample from the US (n = 174). Two observers, blinded from demographic information associated with each specimen, collected the DSP2 metrics. Intra- and interobserver error analyses showed acceptable levels of agreement for all measurements, except for IIMT. Classification accuracies exceeded 95%, with minimal sex bias, for both observers and using various measurement combinations; however, an inclusivity sex bias occurred with more males reaching the 0.95 posterior probability threshold required by DSP2 to provide a sex classification estimate. Based on its high accuracy, forensic anthropologists in the US may consider incorporating DSP2 into their casework, although we recommend excluding IIMT and using SPU with caution. Additional methods will continue to be needed when the posterior probability threshold is not reached.
{"title":"Sex estimation using metrics of the innominate: A test of the DSP2 method.","authors":"Kate M Lesciotto, Alexandra R Klales","doi":"10.1111/1556-4029.15645","DOIUrl":"https://doi.org/10.1111/1556-4029.15645","url":null,"abstract":"<p><p>Sex estimation is a critical component of the biological profile, and forensic anthropologists may use a variety of sex estimation methods depending upon the degree of completeness and state of preservation of the skeletal remains being analyzed. The innominate is widely accepted to be the most sexually dimorphic skeletal element. The Diagnose Sexuelle Probabiliste (DSP) method, which uses 10 measurements of the innominate, was introduced in 2005 and updated as DSP2 in 2017. While DSP2 has been reported to have high classification accuracy rates in studies of South American and European populations, the method has not been widely tested in US samples, and few US practitioners incorporate this method into their casework. The goal of this study was to test the reliability and accuracy of DSP2 using a large, modern sample from the US (n = 174). Two observers, blinded from demographic information associated with each specimen, collected the DSP2 metrics. Intra- and interobserver error analyses showed acceptable levels of agreement for all measurements, except for IIMT. Classification accuracies exceeded 95%, with minimal sex bias, for both observers and using various measurement combinations; however, an inclusivity sex bias occurred with more males reaching the 0.95 posterior probability threshold required by DSP2 to provide a sex classification estimate. Based on its high accuracy, forensic anthropologists in the US may consider incorporating DSP2 into their casework, although we recommend excluding IIMT and using SPU with caution. Additional methods will continue to be needed when the posterior probability threshold is not reached.</p>","PeriodicalId":94080,"journal":{"name":"Journal of forensic sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142515392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Wade Myers, Heng Choon Oliver Chan, Mark Safarik, Zain Khalid, Eleanor Vo
This study of 21 youthful parricide offenders (YPOs) ages 11-21 examined the relationship between psychopathology, level of psychopathy, and crime scene behaviors, particularly two forms of "body management": (1) "body movement" and (2) "body alteration." Undoing, a form of body alteration reflecting psychological rather than practical needs, for example, to lessen guilt or symbolically reverse the killing, was present in one-third of the sample. The sample shared common characteristics with juvenile parricide offenders overall in that most of them killed one parent, were victims of chronic child abuse, had one or more psychiatric diagnoses (PTSD was most prevalent), used a firearm, and committed the killings at the family home. Unexpectedly, YPOs who were child abuse victims were not more likely to engage in undoing behaviors than non-abused youth, and over half of the youth with undoing behaviors had elevated psychopathy levels. Three-quarters of youth who moved victim bodies following the murders had elevated psychopathy levels. Similarly, all three youth who transported bodies away from the crime scene had elevated psychopathy levels and conduct disorder. None of the youth with psychotic symptoms engaged in undoing behaviors, altered victim bodies, moved bodies either within or away from the home, or had an elevated degree of psychopathy; they also most often killed using bladed weapons, whereas the nonpsychotic youth usually used firearms. Studies examining the influence of mental illness and psychopathy level on parricidal behaviors by youth are scarce, and to our knowledge this is the largest study to date investigating this area.
{"title":"Psychopathology, psychopathy, body management, and undoing in youthful parricide offenders.","authors":"Wade Myers, Heng Choon Oliver Chan, Mark Safarik, Zain Khalid, Eleanor Vo","doi":"10.1111/1556-4029.15636","DOIUrl":"https://doi.org/10.1111/1556-4029.15636","url":null,"abstract":"<p><p>This study of 21 youthful parricide offenders (YPOs) ages 11-21 examined the relationship between psychopathology, level of psychopathy, and crime scene behaviors, particularly two forms of \"body management\": (1) \"body movement\" and (2) \"body alteration.\" Undoing, a form of body alteration reflecting psychological rather than practical needs, for example, to lessen guilt or symbolically reverse the killing, was present in one-third of the sample. The sample shared common characteristics with juvenile parricide offenders overall in that most of them killed one parent, were victims of chronic child abuse, had one or more psychiatric diagnoses (PTSD was most prevalent), used a firearm, and committed the killings at the family home. Unexpectedly, YPOs who were child abuse victims were not more likely to engage in undoing behaviors than non-abused youth, and over half of the youth with undoing behaviors had elevated psychopathy levels. Three-quarters of youth who moved victim bodies following the murders had elevated psychopathy levels. Similarly, all three youth who transported bodies away from the crime scene had elevated psychopathy levels and conduct disorder. None of the youth with psychotic symptoms engaged in undoing behaviors, altered victim bodies, moved bodies either within or away from the home, or had an elevated degree of psychopathy; they also most often killed using bladed weapons, whereas the nonpsychotic youth usually used firearms. Studies examining the influence of mental illness and psychopathy level on parricidal behaviors by youth are scarce, and to our knowledge this is the largest study to date investigating this area.</p>","PeriodicalId":94080,"journal":{"name":"Journal of forensic sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142515391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
For decades, detectives and forensic scientists have relied on friction ridge skin comparisons to identify individuals in criminal investigations. This method involves examining (latent) fingermarks, typically captured as two-dimensional (2D) images, and comparing them with known inked impressions or scans. The comparisons focus on general patterns, the location and orientation of minutiae, and additional characteristics like scars or pores. However, this common process overlooks other potentially valuable factors, such as handedness, biological sex, and the time since deposition, which could provide crucial investigative leads, especially in cases with partial or unclear ridge patterns. This study explored the potential of three-dimensional (3D) optical profilometry (OP) to observe changes in fingermarks ridge height and volume over 7 days while considering hand dominance and biological sex. The results indicated that all the assessed 3D metrics were generally consistent in detecting the effects of sex and handedness, mostly noticeable on aged fingermarks. Specifically, females and impressions from their non-dominant hand exhibited higher values. Additionally, the influence of time was evident, with fresh fingermarks showing significantly larger ridges in terms of height and volume. These findings suggest that 3D OP can enhance the probative value of fingermarks by inferring additional characteristics such as sex, handedness, and the age of the impression. These activity-level propositions could provide useful information in cases where only partial or poor-quality fingermarks are recovered, providing new data dimensions that could lead to more comprehensive forensic examinations.
几十年来,刑侦人员和法医学家在刑事调查中一直依靠摩擦纹皮肤对比来识别个人身份。这种方法包括检查(潜伏)指痕(通常以二维(2D)图像的形式捕捉),并将其与已知的墨迹或扫描图像进行比较。比较的重点是一般模式、细微特征的位置和方向,以及疤痕或毛孔等其他特征。然而,这种常见的方法忽略了其他潜在的有价值因素,如手型、生理性别和沉积后的时间,而这些因素可以提供重要的调查线索,尤其是在纹脊图案不完整或不清晰的情况下。本研究探讨了三维(3D)光学轮廓测量法(OP)的潜力,以观察指痕脊高度和体积在 7 天内的变化,同时考虑手的优势和生理性别。结果表明,所有评估的三维指标在检测性别和手性的影响方面基本一致,尤其是对老年指痕的影响更为明显。具体来说,女性和非惯用手的指印显示出更高的数值。此外,时间的影响也很明显,就高度和体积而言,新鲜指印的脊明显更大。这些研究结果表明,3D OP 可以通过推断指印的其他特征(如性别、手型和年龄)来提高指印的证明价值。在只能找到部分指痕或质量较差的指痕的情况下,这些活动层面的命题可以提供有用的信息,提供新的数据维度,从而进行更全面的法医检查。
{"title":"The effect of handedness and biological sex on fingermark ridge height and volume as examined by 3D imaging.","authors":"Josep De Alcaraz-Fossoul, Katherine E Dennehy","doi":"10.1111/1556-4029.15635","DOIUrl":"https://doi.org/10.1111/1556-4029.15635","url":null,"abstract":"<p><p>For decades, detectives and forensic scientists have relied on friction ridge skin comparisons to identify individuals in criminal investigations. This method involves examining (latent) fingermarks, typically captured as two-dimensional (2D) images, and comparing them with known inked impressions or scans. The comparisons focus on general patterns, the location and orientation of minutiae, and additional characteristics like scars or pores. However, this common process overlooks other potentially valuable factors, such as handedness, biological sex, and the time since deposition, which could provide crucial investigative leads, especially in cases with partial or unclear ridge patterns. This study explored the potential of three-dimensional (3D) optical profilometry (OP) to observe changes in fingermarks ridge height and volume over 7 days while considering hand dominance and biological sex. The results indicated that all the assessed 3D metrics were generally consistent in detecting the effects of sex and handedness, mostly noticeable on aged fingermarks. Specifically, females and impressions from their non-dominant hand exhibited higher values. Additionally, the influence of time was evident, with fresh fingermarks showing significantly larger ridges in terms of height and volume. These findings suggest that 3D OP can enhance the probative value of fingermarks by inferring additional characteristics such as sex, handedness, and the age of the impression. These activity-level propositions could provide useful information in cases where only partial or poor-quality fingermarks are recovered, providing new data dimensions that could lead to more comprehensive forensic examinations.</p>","PeriodicalId":94080,"journal":{"name":"Journal of forensic sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142484304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Erica L Romsos, Lisa A Borsuk, Carolyn R Steffen, Sarah Riman, Kevin M Kiesler, Peter M Vallone
Advancements in forensic DNA typing technology and methods have increased sensitivity and, while beneficial, carry the weight of more challenging profile interpretation. In response, the forensic DNA community has often requested more complex reference materials to address commonly encountered measurement and interpretation issues such as complex DNA mixtures, DNA degradation, and PCR inhibition. The National Institute of Standards and Technology (NIST) released Research Grade Test Material 10235: Forensic DNA Typing Resource Samples to support the forensic DNA community. Components include three single source samples, two degraded samples, and three mixture samples. As part of the Research Grade Test Material (RGTM) process, automated methods for bottling, alternative sample tube types, and the addition of carrier RNA for stabilizing low-quantity samples were investigated. Both internal and external testing demonstrate the stability of the material over time at 4°C through qPCR testing. In the development of a data portal, users have been allowed to anonymously upload results and compare their data with NIST and others. This report describes the preparation and stability of this material.
法医 DNA 分型技术和方法的进步提高了灵敏度,在带来好处的同时,也带来了更具挑战性的图谱解读。为此,法医 DNA 社区经常需要更复杂的参考材料,以解决常见的测量和解释问题,如复杂的 DNA 混合物、DNA 降解和 PCR 抑制等。美国国家标准与技术研究院(NIST)发布了研究级测试材料 10235:法医 DNA 分型资源样本》,为法医 DNA 界提供支持。样本包括三个单一来源样本、两个降解样本和三个混合物样本。作为研究级测试材料(RGTM)流程的一部分,研究了自动装瓶方法、替代样本管类型以及添加载体 RNA 以稳定低量样本的方法。通过 qPCR 测试,内部和外部测试都证明了材料在 4°C 温度下的长期稳定性。在数据门户网站的开发过程中,用户可以匿名上传结果,并将其数据与 NIST 和其他机构的数据进行比较。本报告介绍了这种材料的制备和稳定性。
{"title":"Development of a forensic DNA research grade test material.","authors":"Erica L Romsos, Lisa A Borsuk, Carolyn R Steffen, Sarah Riman, Kevin M Kiesler, Peter M Vallone","doi":"10.1111/1556-4029.15639","DOIUrl":"https://doi.org/10.1111/1556-4029.15639","url":null,"abstract":"<p><p>Advancements in forensic DNA typing technology and methods have increased sensitivity and, while beneficial, carry the weight of more challenging profile interpretation. In response, the forensic DNA community has often requested more complex reference materials to address commonly encountered measurement and interpretation issues such as complex DNA mixtures, DNA degradation, and PCR inhibition. The National Institute of Standards and Technology (NIST) released Research Grade Test Material 10235: Forensic DNA Typing Resource Samples to support the forensic DNA community. Components include three single source samples, two degraded samples, and three mixture samples. As part of the Research Grade Test Material (RGTM) process, automated methods for bottling, alternative sample tube types, and the addition of carrier RNA for stabilizing low-quantity samples were investigated. Both internal and external testing demonstrate the stability of the material over time at 4°C through qPCR testing. In the development of a data portal, users have been allowed to anonymously upload results and compare their data with NIST and others. This report describes the preparation and stability of this material.</p>","PeriodicalId":94080,"journal":{"name":"Journal of forensic sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142484302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
During the analysis of a commingled human remains assemblage, pair-matching is often conducted as part of the inventory to inform the DNA sampling strategy and to calculate the minimum or most likely number of individuals. As commingled assemblages become larger, pair-matching becomes more difficult, and it is unknown whether accuracy declines. Therefore, a study to determine the accuracy rates of visual pair-matching for multiple observers was conducted on a large, commingled human assemblage. The sample consisted of 580 left and right humeri (n = 287 and n = 293, respectively) from the commingled remains of the USS Oklahoma, which were undergoing analysis at the Defense POW/MIA Accounting Agency. Five anthropologists completed this study, determining humeri pairs with varying degrees of confidence and non-pairs. The overall precision was 81.0% and ranged from 74.1% to 95.9% for participants, including all confidence levels. When considering only confident matches, the overall precision for all participants increased to 91.4%.
{"title":"Results of a large-scale humeri pair matching study.","authors":"Carrie B LeGarde","doi":"10.1111/1556-4029.15642","DOIUrl":"https://doi.org/10.1111/1556-4029.15642","url":null,"abstract":"<p><p>During the analysis of a commingled human remains assemblage, pair-matching is often conducted as part of the inventory to inform the DNA sampling strategy and to calculate the minimum or most likely number of individuals. As commingled assemblages become larger, pair-matching becomes more difficult, and it is unknown whether accuracy declines. Therefore, a study to determine the accuracy rates of visual pair-matching for multiple observers was conducted on a large, commingled human assemblage. The sample consisted of 580 left and right humeri (n = 287 and n = 293, respectively) from the commingled remains of the USS Oklahoma, which were undergoing analysis at the Defense POW/MIA Accounting Agency. Five anthropologists completed this study, determining humeri pairs with varying degrees of confidence and non-pairs. The overall precision was 81.0% and ranged from 74.1% to 95.9% for participants, including all confidence levels. When considering only confident matches, the overall precision for all participants increased to 91.4%.</p>","PeriodicalId":94080,"journal":{"name":"Journal of forensic sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142484303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Individuals who commit criminal behaviors are often thought to prioritize short-term goals rather than long-term goals (i.e., a present vs. a future time orientation). Though previous theories of crime and empirical research support a relationship among future time orientation, criminal thinking, and illegal behaviors, there is disagreement in the literature about how to operationalize the multidimensional construct of future time orientation. The primary aim of this pre-registered survey study was to measure multiple components of future time orientation to better understand the relationship with criminality-related outcomes. Participants were 248 undergraduate college students. Survey materials included a measure of impulsivity (Barratt Impulsiveness Scale-11), self-control (Brief Self-Control Scale), delay discounting (Monetary Choice Questionnaire), and Carstensen's Future Time Perspective Scale. Participants also completed two measures of criminal thinking style (the Texas Christian University Criminal Thinking Scale, the Criminogenic Cognitions Scale) and an illegal behaviors checklist. Bivariate results demonstrated a negative relationship between future time orientation (i.e., low impulsivity, high self-control, high future time perspective) and criminal thinking style. The relationship between delay discounting and criminal thinking was in the hypothesized direction but failed to reach statistical significance. Multiple regression analyses indicated that the measure of self-control had the most consistent and incrementally significant relationship with both criminal thinking style and illegal behaviors. Theoretical and practical implications of the results are discussed along with study limitations and future directions.
{"title":"The relationship between four indicators of future time orientation, criminal thinking style, and illegal behaviors.","authors":"Danielle Squillaro, Michael T Bixter","doi":"10.1111/1556-4029.15640","DOIUrl":"https://doi.org/10.1111/1556-4029.15640","url":null,"abstract":"<p><p>Individuals who commit criminal behaviors are often thought to prioritize short-term goals rather than long-term goals (i.e., a present vs. a future time orientation). Though previous theories of crime and empirical research support a relationship among future time orientation, criminal thinking, and illegal behaviors, there is disagreement in the literature about how to operationalize the multidimensional construct of future time orientation. The primary aim of this pre-registered survey study was to measure multiple components of future time orientation to better understand the relationship with criminality-related outcomes. Participants were 248 undergraduate college students. Survey materials included a measure of impulsivity (Barratt Impulsiveness Scale-11), self-control (Brief Self-Control Scale), delay discounting (Monetary Choice Questionnaire), and Carstensen's Future Time Perspective Scale. Participants also completed two measures of criminal thinking style (the Texas Christian University Criminal Thinking Scale, the Criminogenic Cognitions Scale) and an illegal behaviors checklist. Bivariate results demonstrated a negative relationship between future time orientation (i.e., low impulsivity, high self-control, high future time perspective) and criminal thinking style. The relationship between delay discounting and criminal thinking was in the hypothesized direction but failed to reach statistical significance. Multiple regression analyses indicated that the measure of self-control had the most consistent and incrementally significant relationship with both criminal thinking style and illegal behaviors. Theoretical and practical implications of the results are discussed along with study limitations and future directions.</p>","PeriodicalId":94080,"journal":{"name":"Journal of forensic sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142484305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}