Purpose: To evaluate the antibiofilm action of 2.5 mg/mL peracetic acid (PA), 0.5 mg/mL cetylpyridinium chloride (CPC), and 160 mg/mL N-acetylcysteine (NAC) against multispecies biofilm of Streptococcus mutans, Staphylococcus aureus, Candida albicans, and Candida glabrata developed on surfaces of heat-polymerizing acrylic resin (AR) and cobalt-chromium (Co-Cr) alloy.
Materials and methods: A multispecies biofilm was grown on the surface of AR and Co-Cr specimens (Ø 12 × 3 mm). After biofilm maturation, the specimens were immersed in experimental solutions and evaluated through biofilm viability (CFU; n = 9), biofilm metabolic activity (XTT; n = 9), biofilm-covered areas (live/dead; n = 2), effects on the extracellular polymeric substance (EPS; n = 2), and biofilm morphology (n = 1). Data were analyzed by ANOVA and Tukey post-hoc test or Kruskal-Wallis followed by Dunn post-hoc test (α = .05).
Results: Overall, all evaluated solutions impacted biofilm viability. PA presented wider activity by reducing CFU of all microorganisms on both surfaces, XTT (P < .001) and live/dead (P < .001). NAC had a notorious effect in reducing the viability of bacteria without affecting the yeasts. NAC reduced XTT on AR (P = .006) and Co-Cr (P = .003) but did not reduce the aggregated biofilm layer. CPC had a distinct effect, being most effective in reducing CFU on AR compared to the Co-Cr surface. However, it did not influence XTT or the amount of residual aggregated biofilm.
Conclusions: PA provided the greatest antibiofilm action, while CPC and NAC showed intermediate action. Nonetheless, no solution was able to completely remove the biofilm adhered to the surfaces of heat-polymerizing AR and Co-Cr alloy.
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