Pub Date : 2002-01-01DOI: 10.1615/JENVIRONPATHOLTOXICOLONCOL.V21.I2.110
Takashi Takahashi, K. Kozaki, Y. Yatabe, H. Achiwa, T. Hida
It is well accepted that an increase in the expression of cyclooxygenase-2 (COX-2), a key inducible enzyme involved in the production of prostaglandins and other eicosanoids, may play a significant role in carcinogenesis in addition to its well-known role in inflammatory reactions. Whereas previous studies were largely confined to colorectal tumorigenesis, we have shown that a significantly increased expression of COX-2 may also play a role in the development of lung cancer. COX-2 expression was found to be frequently elevated in lung cancer, especially in adenocarcinoma, and the proportion of lung cancer cells with marked COX-2 expression was much higher in lymph node metastases than in the corresponding primary tumors. It was also shown that early stage adenocarcinoma patients with increased COX-2 expression who were surgically treated had a shorter survival. Our studies, which used high- and low-metastatic human lung cancer cell sublines established in our laboratory, revealed an association between metastatic capabilities and COX-2 expression levels: COX-2-specific inhibitors could inhibit in vitro the invasion of the highly metastatic NCI-H460-LNM35 clone through Matrigel-containing basement membrane components as well as the spontaneous in vivo metastasis in SCID mice. Taken together, these findings suggest that an increase in COX-2 expression maybe associated with the development of lung cancer and possibly with the acquisition of an invasive and metastatic phenotype.
{"title":"Increased expression of COX-2 in the development of human lung cancers.","authors":"Takashi Takahashi, K. Kozaki, Y. Yatabe, H. Achiwa, T. Hida","doi":"10.1615/JENVIRONPATHOLTOXICOLONCOL.V21.I2.110","DOIUrl":"https://doi.org/10.1615/JENVIRONPATHOLTOXICOLONCOL.V21.I2.110","url":null,"abstract":"It is well accepted that an increase in the expression of cyclooxygenase-2 (COX-2), a key inducible enzyme involved in the production of prostaglandins and other eicosanoids, may play a significant role in carcinogenesis in addition to its well-known role in inflammatory reactions. Whereas previous studies were largely confined to colorectal tumorigenesis, we have shown that a significantly increased expression of COX-2 may also play a role in the development of lung cancer. COX-2 expression was found to be frequently elevated in lung cancer, especially in adenocarcinoma, and the proportion of lung cancer cells with marked COX-2 expression was much higher in lymph node metastases than in the corresponding primary tumors. It was also shown that early stage adenocarcinoma patients with increased COX-2 expression who were surgically treated had a shorter survival. Our studies, which used high- and low-metastatic human lung cancer cell sublines established in our laboratory, revealed an association between metastatic capabilities and COX-2 expression levels: COX-2-specific inhibitors could inhibit in vitro the invasion of the highly metastatic NCI-H460-LNM35 clone through Matrigel-containing basement membrane components as well as the spontaneous in vivo metastasis in SCID mice. Taken together, these findings suggest that an increase in COX-2 expression maybe associated with the development of lung cancer and possibly with the acquisition of an invasive and metastatic phenotype.","PeriodicalId":94332,"journal":{"name":"Journal of environmental pathology, toxicology and oncology : official organ of the International Society for Environmental Toxicology and Cancer","volume":"0602 1","pages":"177-81"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83024706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2002-01-01DOI: 10.1615/JENVIRONPATHOLTOXICOLONCOL.V21.I3.20
Jasbir Singh, Kovleen, H. M. Dani, Rajeshwar Sharmar
Chemical carcinogens are organ selective and not organ specific. Microsomal degranulation elution profiles employing sepharose CL-2B gel filtration for the separation of microsomes from different organs of rats before and after treatment with some known chemical carcinogens have shown for the first time that carcinogens selectively detach maximum ribosomes from rough reticular membranes of their target organs. The detachment of ribosomes from other organs varies, but is comparatively lesser than that in the target organs. A chemical carcinogen might, therefore, be tumorigenic for several organs, possibly depending on the dose reaching a particular organ and its activation to the ultimate form of the carcinogen.
{"title":"Organ selectivity of chemical carcinogens.","authors":"Jasbir Singh, Kovleen, H. M. Dani, Rajeshwar Sharmar","doi":"10.1615/JENVIRONPATHOLTOXICOLONCOL.V21.I3.20","DOIUrl":"https://doi.org/10.1615/JENVIRONPATHOLTOXICOLONCOL.V21.I3.20","url":null,"abstract":"Chemical carcinogens are organ selective and not organ specific. Microsomal degranulation elution profiles employing sepharose CL-2B gel filtration for the separation of microsomes from different organs of rats before and after treatment with some known chemical carcinogens have shown for the first time that carcinogens selectively detach maximum ribosomes from rough reticular membranes of their target organs. The detachment of ribosomes from other organs varies, but is comparatively lesser than that in the target organs. A chemical carcinogen might, therefore, be tumorigenic for several organs, possibly depending on the dose reaching a particular organ and its activation to the ultimate form of the carcinogen.","PeriodicalId":94332,"journal":{"name":"Journal of environmental pathology, toxicology and oncology : official organ of the International Society for Environmental Toxicology and Cancer","volume":"15 1","pages":"213-21"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75353633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2002-01-01DOI: 10.1615/JENVIRONPATHOLTOXICOLONCOL.V21.I1.50
P. Gajalakshmi, P. Mallick, P. Venkatesan, S. T. Santhiya, A. Ramesh
In a comparative study of sister chromatid exchanges (SCEs) in cultured lymphocytes, we evaluated the genotoxic risk in 104 male spray painters employed in repair workshops in Chennai City, India, and 50 matched healthy, unexposed controls. We found a higher frequency of SCEs among painters (3.74 +/- 0.11, mean +/- SE) than among controls (2.15 +/- 0.08), and among smoking painters (4.03 +/- 0.21) than among nonsmoking painters (3.55 +/- 0.13), with no significant difference in controls (smokers: 2.1 +/- 0.2; nonsmokers: 2.2 +/- 0.1). Alcoholism did not contribute to an increased SCE frequency. Stepwise multiple linear regression analysis on painters showed that duration of service, smoking, and alcoholism significantly affected SCE scores and explained the 14% variation observed.
{"title":"Studies on sister chromatid exchanges in peripheral lymphocytes of spray painters.","authors":"P. Gajalakshmi, P. Mallick, P. Venkatesan, S. T. Santhiya, A. Ramesh","doi":"10.1615/JENVIRONPATHOLTOXICOLONCOL.V21.I1.50","DOIUrl":"https://doi.org/10.1615/JENVIRONPATHOLTOXICOLONCOL.V21.I1.50","url":null,"abstract":"In a comparative study of sister chromatid exchanges (SCEs) in cultured lymphocytes, we evaluated the genotoxic risk in 104 male spray painters employed in repair workshops in Chennai City, India, and 50 matched healthy, unexposed controls. We found a higher frequency of SCEs among painters (3.74 +/- 0.11, mean +/- SE) than among controls (2.15 +/- 0.08), and among smoking painters (4.03 +/- 0.21) than among nonsmoking painters (3.55 +/- 0.13), with no significant difference in controls (smokers: 2.1 +/- 0.2; nonsmokers: 2.2 +/- 0.1). Alcoholism did not contribute to an increased SCE frequency. Stepwise multiple linear regression analysis on painters showed that duration of service, smoking, and alcoholism significantly affected SCE scores and explained the 14% variation observed.","PeriodicalId":94332,"journal":{"name":"Journal of environmental pathology, toxicology and oncology : official organ of the International Society for Environmental Toxicology and Cancer","volume":"74 1","pages":"63-5"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88950348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2002-01-01DOI: 10.1615/JENVIRONPATHOLTOXICOLONCOL.V21.I1.40
M. Shamy, Hazem H Osman, K. Kandeel, N. M. Abdel-Moneim, Said Khalid F El
Styrene is a known mutagen and suspected carcinogen, used in the reinforced plastic industry. This study aims to identify the occurrence of DNA single strand breaks (SSBs) in workers exposed to styrene levels far below the recommended standards. We compared 26 exposed workers with 26 control subjects and found a significant increase in the incidence of DNA-SSBs in the exposed individuals. The levels of the biological indices of exposure (urinary mandelic and phenyl glyoxylic acids) were less than 25% of the recommended limits. Reduction of the threshold limit values/time-weighted-average (TLV-TWA) applied is strongly recommended.
{"title":"DNA single strand breaks induced by low levels of occupational exposure to styrene: the gap between standards and reality.","authors":"M. Shamy, Hazem H Osman, K. Kandeel, N. M. Abdel-Moneim, Said Khalid F El","doi":"10.1615/JENVIRONPATHOLTOXICOLONCOL.V21.I1.40","DOIUrl":"https://doi.org/10.1615/JENVIRONPATHOLTOXICOLONCOL.V21.I1.40","url":null,"abstract":"Styrene is a known mutagen and suspected carcinogen, used in the reinforced plastic industry. This study aims to identify the occurrence of DNA single strand breaks (SSBs) in workers exposed to styrene levels far below the recommended standards. We compared 26 exposed workers with 26 control subjects and found a significant increase in the incidence of DNA-SSBs in the exposed individuals. The levels of the biological indices of exposure (urinary mandelic and phenyl glyoxylic acids) were less than 25% of the recommended limits. Reduction of the threshold limit values/time-weighted-average (TLV-TWA) applied is strongly recommended.","PeriodicalId":94332,"journal":{"name":"Journal of environmental pathology, toxicology and oncology : official organ of the International Society for Environmental Toxicology and Cancer","volume":"47 1","pages":"57-61"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87419911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2002-01-01DOI: 10.1615/JENVIRONPATHOLTOXICOLONCOL.V21.I2.70
S. Lee, C. Hong, S. Huh, Sun-Sook Kim, Omer Oh, H. Min, Kwang-Kyun Park, W. Chung, J. Hwang
Prostaglandins and nitric oxide produced by inducible cyclooygenase (COX-2) and nitric oxide synthase (iNOS), respectively, have been implicated as important mediators in the processes of inflammation and carcinogenesis. These potential COX-2 and iNOS inhibitors have been considered as antiinflammatory and cancer chemopreventive agents. In this study, we investigated the effect of natural sesquiterpenoids isolated from plants of the Zingiberaceae family on the activities of COX-2 and iNOS in cultured lipopolysaccharide (LPS)-activated mouse macrophage cell RAW 264.7 to discover new lead compounds as COX-2 or iNOS inhibitors. Xanthorrhizol, a sesquiterpenoid, isolated from the rhizome of Curcuma xanthorrhiza Roxb. (Zingiberaceae), exhibited a potent inhibition of COX-2 (IC50 = 0.2 microg/mL) and iNOS activity (IC50 = 1.0 microg/mL) in the assay system of prostaglandin E2 (PGE2) accumulation and nitric oxide production, respectively. Western blot analyses revealed that the inhibitory potential of xanthorrhizol on the COX-2 activity coincided well with the suppression of COX-2 protein expression in LPS-induced macrophages. In addition, sesquiterpenoids beta-turmerone and ar-turmerone isolated from the rhizome of Curcuma zedoaria Roscoe (Zingiberaceae) also showed a potent inhibitory activity of COX-2 (beta-turmerone, IC50 = 1.6 microg/mL; ar-turmerone, IC50 = 5.2 microg/mL) and iNOS (beta-turmerone, IC50 = 4.6 microg/mL; ar-turmerone, IC50 = 3.2 microg/mL). These results suggest that natural sesquiterpenoids from C. xanthorrhiza and C. zedoaria might be lead candidates for further developing COX-2 or iNOS inhibitors possessing cancer chemopreventive or anti-inflammatory properties.
{"title":"Suppressive effect of natural sesquiterpenoids on inducible cyclooxygenase (COX-2) and nitric oxide synthase (iNOS) activity in mouse macrophage cells.","authors":"S. Lee, C. Hong, S. Huh, Sun-Sook Kim, Omer Oh, H. Min, Kwang-Kyun Park, W. Chung, J. Hwang","doi":"10.1615/JENVIRONPATHOLTOXICOLONCOL.V21.I2.70","DOIUrl":"https://doi.org/10.1615/JENVIRONPATHOLTOXICOLONCOL.V21.I2.70","url":null,"abstract":"Prostaglandins and nitric oxide produced by inducible cyclooygenase (COX-2) and nitric oxide synthase (iNOS), respectively, have been implicated as important mediators in the processes of inflammation and carcinogenesis. These potential COX-2 and iNOS inhibitors have been considered as antiinflammatory and cancer chemopreventive agents. In this study, we investigated the effect of natural sesquiterpenoids isolated from plants of the Zingiberaceae family on the activities of COX-2 and iNOS in cultured lipopolysaccharide (LPS)-activated mouse macrophage cell RAW 264.7 to discover new lead compounds as COX-2 or iNOS inhibitors. Xanthorrhizol, a sesquiterpenoid, isolated from the rhizome of Curcuma xanthorrhiza Roxb. (Zingiberaceae), exhibited a potent inhibition of COX-2 (IC50 = 0.2 microg/mL) and iNOS activity (IC50 = 1.0 microg/mL) in the assay system of prostaglandin E2 (PGE2) accumulation and nitric oxide production, respectively. Western blot analyses revealed that the inhibitory potential of xanthorrhizol on the COX-2 activity coincided well with the suppression of COX-2 protein expression in LPS-induced macrophages. In addition, sesquiterpenoids beta-turmerone and ar-turmerone isolated from the rhizome of Curcuma zedoaria Roscoe (Zingiberaceae) also showed a potent inhibitory activity of COX-2 (beta-turmerone, IC50 = 1.6 microg/mL; ar-turmerone, IC50 = 5.2 microg/mL) and iNOS (beta-turmerone, IC50 = 4.6 microg/mL; ar-turmerone, IC50 = 3.2 microg/mL). These results suggest that natural sesquiterpenoids from C. xanthorrhiza and C. zedoaria might be lead candidates for further developing COX-2 or iNOS inhibitors possessing cancer chemopreventive or anti-inflammatory properties.","PeriodicalId":94332,"journal":{"name":"Journal of environmental pathology, toxicology and oncology : official organ of the International Society for Environmental Toxicology and Cancer","volume":"10 1","pages":"141-8"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91180573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2002-01-01DOI: 10.1615/JENVIRONPATHOLTOXICOLONCOL.V21.I3.50
A. Rajpurkar, Yang Jiang, C. Dhabuwala, J. Dunbar, Haikun Li
In a previous studywe demonstrated the deleterious effect of cigarette smoke on spermatogenesis in the testis of peripubertal Sprague-Dawley rats. In this study we investigated the development of apoptosis as a possible contributing factor to the pathogenic mechanism underlying these effects. Peripubertal rats were exposed to cigarette smoke with the Walton Horizontal Smoking Machine. Similarly, age-matched control rats were exposed to room air with the smoking machine. Rats from both groups were sacrificed after 45 days of treatment and the testes were removed. Testes were stained utilizing the terminal deoxynucleotidyl transferase dUTP nick end-labeling (TUNEL) staining technique. DNA fragmentation was further evaluated using gelectrophoresis. There was a significant increase in the incidence of apoptosis in the treated group compared to the control group as demonstrated by the larger amount of tubules containing > or = 3apoptotic bodies in the smoke-exposed group, that is, 36% versus 14% in the control group (p < 0.05). Agarose gel electrophoresis demonstrated the DNA ladder in the treated group but not in the control animals. In conclusion, chronic cigarette smoke induces apoptosis in the rat testis. Apoptosis may be one of the pathogenic mechanisms responsible for defective spermatogenesis in the rat following chronic cigarette smoking.
{"title":"Cigarette smoking induces apoptosis in rat testis.","authors":"A. Rajpurkar, Yang Jiang, C. Dhabuwala, J. Dunbar, Haikun Li","doi":"10.1615/JENVIRONPATHOLTOXICOLONCOL.V21.I3.50","DOIUrl":"https://doi.org/10.1615/JENVIRONPATHOLTOXICOLONCOL.V21.I3.50","url":null,"abstract":"In a previous studywe demonstrated the deleterious effect of cigarette smoke on spermatogenesis in the testis of peripubertal Sprague-Dawley rats. In this study we investigated the development of apoptosis as a possible contributing factor to the pathogenic mechanism underlying these effects. Peripubertal rats were exposed to cigarette smoke with the Walton Horizontal Smoking Machine. Similarly, age-matched control rats were exposed to room air with the smoking machine. Rats from both groups were sacrificed after 45 days of treatment and the testes were removed. Testes were stained utilizing the terminal deoxynucleotidyl transferase dUTP nick end-labeling (TUNEL) staining technique. DNA fragmentation was further evaluated using gelectrophoresis. There was a significant increase in the incidence of apoptosis in the treated group compared to the control group as demonstrated by the larger amount of tubules containing > or = 3apoptotic bodies in the smoke-exposed group, that is, 36% versus 14% in the control group (p < 0.05). Agarose gel electrophoresis demonstrated the DNA ladder in the treated group but not in the control animals. In conclusion, chronic cigarette smoke induces apoptosis in the rat testis. Apoptosis may be one of the pathogenic mechanisms responsible for defective spermatogenesis in the rat following chronic cigarette smoking.","PeriodicalId":94332,"journal":{"name":"Journal of environmental pathology, toxicology and oncology : official organ of the International Society for Environmental Toxicology and Cancer","volume":"88 1","pages":"243-8"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81306084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2002-01-01DOI: 10.1615/JENVIRONPATHOLTOXICOLONCOL.V21.I3.40
David Shi, B. Jiang
Reactive oxygen species (ROS) have been shown to cause a broad spectrum of damage to the biological system. ROS-mediated reactions are believed to play a key role in Cr(VI)-induced carcinogenesis. Using electron spin resonance (ESR) spin trapping, the present study shows that apple juice efficiently scavenged hydroxyl radicals generated by Cr(VI) reduction catalyzed byglutathione reductase/NADPH. Apple juice reduced Cr(VI)-induced lipid peroxidation, DNA damage, cell apoptosis, and NF-kappaB activation in human lung epithelial A549 cells. The lipid peroxidation was measured by a colorimetric assay, DNA damage by single cell gel electrophoresis assay, induction of cell apoptosis by flow cytometry, and activation of NF-kappaB by luciferase assay. The results show that through its antioxidant properties, apple juice can protect Cr(VI)-induced cellular injury and may help reduce its carcinogenic potentiaL
{"title":"Antioxidant properties of apple juice and its protection against Cr(VI)-induced cellular injury.","authors":"David Shi, B. Jiang","doi":"10.1615/JENVIRONPATHOLTOXICOLONCOL.V21.I3.40","DOIUrl":"https://doi.org/10.1615/JENVIRONPATHOLTOXICOLONCOL.V21.I3.40","url":null,"abstract":"Reactive oxygen species (ROS) have been shown to cause a broad spectrum of damage to the biological system. ROS-mediated reactions are believed to play a key role in Cr(VI)-induced carcinogenesis. Using electron spin resonance (ESR) spin trapping, the present study shows that apple juice efficiently scavenged hydroxyl radicals generated by Cr(VI) reduction catalyzed byglutathione reductase/NADPH. Apple juice reduced Cr(VI)-induced lipid peroxidation, DNA damage, cell apoptosis, and NF-kappaB activation in human lung epithelial A549 cells. The lipid peroxidation was measured by a colorimetric assay, DNA damage by single cell gel electrophoresis assay, induction of cell apoptosis by flow cytometry, and activation of NF-kappaB by luciferase assay. The results show that through its antioxidant properties, apple juice can protect Cr(VI)-induced cellular injury and may help reduce its carcinogenic potentiaL","PeriodicalId":94332,"journal":{"name":"Journal of environmental pathology, toxicology and oncology : official organ of the International Society for Environmental Toxicology and Cancer","volume":"10 1","pages":"233-42"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78554916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2002-01-01DOI: 10.1615/JENVIRONPATHOLTOXICOLONCOL.V21.I3.70
Y. Shukla, P. Taneja
Cypermethrin (CYP) is a synthetic pyrethroid insecticide considered to be environmentally safe and widely used in agriculture and veterinary medicine. To ascertain the validity of this opinion, we investigated the mutagenic potential of CYP using the dominant lethal assay in male Swiss albino mice. CYP was administered by gavage at the dose of 20, 40, and 80 mg/kg body weight, dissolved in 0.2 mL corn oil. Treated mice from all groups were mated with untreated virgin females for a period of 6 weeks that covers the entirespermatogenetic cycle. In the pregnant females, we found a high rate of pre- and post-implantation losses. Dominant lethal mutations were induced in a benzo(a)pyrene-treated group (positive control), and a reduction in the number of total implants was found in all CYP-treated groups only during the initial mating weeks. No significant pre-implantation losses were noted in any of the tested doses. However, significant postimplantation losses were identified in the medium and high doses of CYP. A dose-dependent decline in the number of living implants was noticed in all CYP-treated animals during the first 3 weeks, but decreased in the later weeks. The average mutagenic index of 6 weeks was significantly increased only in the high CYP dose. Our results showed that CYP has mutagenic activity, inducing dominant lethal mutations in male germ cells of mice and caution is recommended in the use of this insecticide.
{"title":"Mutagenic potential of cypermethrin in mouse dominant lethal assay.","authors":"Y. Shukla, P. Taneja","doi":"10.1615/JENVIRONPATHOLTOXICOLONCOL.V21.I3.70","DOIUrl":"https://doi.org/10.1615/JENVIRONPATHOLTOXICOLONCOL.V21.I3.70","url":null,"abstract":"Cypermethrin (CYP) is a synthetic pyrethroid insecticide considered to be environmentally safe and widely used in agriculture and veterinary medicine. To ascertain the validity of this opinion, we investigated the mutagenic potential of CYP using the dominant lethal assay in male Swiss albino mice. CYP was administered by gavage at the dose of 20, 40, and 80 mg/kg body weight, dissolved in 0.2 mL corn oil. Treated mice from all groups were mated with untreated virgin females for a period of 6 weeks that covers the entirespermatogenetic cycle. In the pregnant females, we found a high rate of pre- and post-implantation losses. Dominant lethal mutations were induced in a benzo(a)pyrene-treated group (positive control), and a reduction in the number of total implants was found in all CYP-treated groups only during the initial mating weeks. No significant pre-implantation losses were noted in any of the tested doses. However, significant postimplantation losses were identified in the medium and high doses of CYP. A dose-dependent decline in the number of living implants was noticed in all CYP-treated animals during the first 3 weeks, but decreased in the later weeks. The average mutagenic index of 6 weeks was significantly increased only in the high CYP dose. Our results showed that CYP has mutagenic activity, inducing dominant lethal mutations in male germ cells of mice and caution is recommended in the use of this insecticide.","PeriodicalId":94332,"journal":{"name":"Journal of environmental pathology, toxicology and oncology : official organ of the International Society for Environmental Toxicology and Cancer","volume":"31 1","pages":"259-65"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74793137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2002-01-01DOI: 10.1615/JENVIRONPATHOLTOXICOLONCOL.V21.I2.20
D. Bishop-Bailey, S. Calatayud, T. Warner, T. Hla, J. Mitchell
Increased expression of inducible cyclooxygenase (COX-2) is associated with a wide variety of tumors. In addition, inhibitors of COX have shown a great deal of promise in vitro and in animal models as potential antitumor therapies. COX enzymes use the substrate arachidonic acid to produce prostaglandin (PG)H2, the precursor to all the prostanoids. Therefore, the release of individual prostanoids depends on the abundance and functional coupling to individual PG synthase isoenzymes. Colony stimulating factors (CSFs) are also potential antitumor agents via their ability to augment the immune response. When COX-2 is expressed, the CSF, granulocyte macrophage (GM)-CSF, and granulocyte (G)-CSF are exquisitely sensitive to endogenous PGs. In addition, the ability of COX-2 to suppress GM-CSF release is mediated via traditional IP/EP prostanoid receptors linked to cAMP-dependent pathways. Therefore, inhibition of COX-2 in tumors may have the important side effect of enhancing the immune response. Recently, novel signaling pathways for PG derivatives have been discovered; in particular the PGD2 dehydration product 15-deoxy-delta(12,14)-(15d)-PGJ2 was identified as a ligand for the nuclear receptor/transcription factor, peroxisome proliferator-activated receptor (PPAR)-gamma. PPARgamma is present at high levels in a number of tumors, and is also present in endothelial cells. 15d-PGJ2 as well as other nonprostanoid PPARgamma ligands are antitumor, and antiangiogenic, by dramatically inhibiting the growth of tumor cells and endothelial cells by either causing terminal differentiation, and/or by inducing apoptosis. We have recently found that, in addition to IP and EP ligands generated by COX-2, PPARgamma ligands similarly inhibit GM-CSF release. Effecting individual prostanoid pathways at the level of COX expression, profile of PG products produced or selective PG receptor activation may produce novel therapies, either dependent or independent of CSF release, to target cancers.
{"title":"Prostaglandins and the regulation of tumor growth.","authors":"D. Bishop-Bailey, S. Calatayud, T. Warner, T. Hla, J. Mitchell","doi":"10.1615/JENVIRONPATHOLTOXICOLONCOL.V21.I2.20","DOIUrl":"https://doi.org/10.1615/JENVIRONPATHOLTOXICOLONCOL.V21.I2.20","url":null,"abstract":"Increased expression of inducible cyclooxygenase (COX-2) is associated with a wide variety of tumors. In addition, inhibitors of COX have shown a great deal of promise in vitro and in animal models as potential antitumor therapies. COX enzymes use the substrate arachidonic acid to produce prostaglandin (PG)H2, the precursor to all the prostanoids. Therefore, the release of individual prostanoids depends on the abundance and functional coupling to individual PG synthase isoenzymes. Colony stimulating factors (CSFs) are also potential antitumor agents via their ability to augment the immune response. When COX-2 is expressed, the CSF, granulocyte macrophage (GM)-CSF, and granulocyte (G)-CSF are exquisitely sensitive to endogenous PGs. In addition, the ability of COX-2 to suppress GM-CSF release is mediated via traditional IP/EP prostanoid receptors linked to cAMP-dependent pathways. Therefore, inhibition of COX-2 in tumors may have the important side effect of enhancing the immune response. Recently, novel signaling pathways for PG derivatives have been discovered; in particular the PGD2 dehydration product 15-deoxy-delta(12,14)-(15d)-PGJ2 was identified as a ligand for the nuclear receptor/transcription factor, peroxisome proliferator-activated receptor (PPAR)-gamma. PPARgamma is present at high levels in a number of tumors, and is also present in endothelial cells. 15d-PGJ2 as well as other nonprostanoid PPARgamma ligands are antitumor, and antiangiogenic, by dramatically inhibiting the growth of tumor cells and endothelial cells by either causing terminal differentiation, and/or by inducing apoptosis. We have recently found that, in addition to IP and EP ligands generated by COX-2, PPARgamma ligands similarly inhibit GM-CSF release. Effecting individual prostanoid pathways at the level of COX expression, profile of PG products produced or selective PG receptor activation may produce novel therapies, either dependent or independent of CSF release, to target cancers.","PeriodicalId":94332,"journal":{"name":"Journal of environmental pathology, toxicology and oncology : official organ of the International Society for Environmental Toxicology and Cancer","volume":"198 1","pages":"93-101"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82810442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2002-01-01DOI: 10.1615/JENVIRONPATHOLTOXICOLONCOL.V21.I1.10
Jeannine H. Powell, P. Gannett
Aryl hydrazines carcinogenesis has been studied for over 25 years and remains poorly understood, although most aryl hydrazines are toxic, tumorigenic, or carcinogenic. In this article, aryl hydrazine carcinogenesis is reviewed comprehensively. The relevant chemistry and biochemistry of aryl hydrazines are first addressed and provide the framework for understanding how aryl hydrazines are metabolized, the reactive intermediates that are produced, and the biological reactive intermediates and products that are formed. Issues of DNA damage, mutagenicity, and enzyme activation are next addressed followed by a brief review of aryl hydrazine tumorigenicity studies. Because several related substrates are metabolized to the same intermediates as are aryl hydrazines, they are briefly discussed. The review concludes with a short discussion of the possible mechanism of carcinogenesis by aryl hydrazines.
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