Lyophilization of finely minced rat skin removed the water, and subsequent extraction with light petroleum at 4° removed the fat. An extracellular, extrafibrillar phase ('ground substance') was extracted with isotonic saline, the cells were dispersed by treatment with trypsin, and the collagen was quantitatively converted to gelatin by autoclaving, leaving a negligible residue. The optimal conditions for each step were ascertained and the compositions of the fractions were examined. An appendix describes a device for the extraction of multiple tissue samples.
{"title":"THE CHEMICAL ANATOMY OF RAT SKIN.","authors":"P. Sweeny, R. H. Pearce, H. G. Vance","doi":"10.1139/Y63-260","DOIUrl":"https://doi.org/10.1139/Y63-260","url":null,"abstract":"Lyophilization of finely minced rat skin removed the water, and subsequent extraction with light petroleum at 4° removed the fat. An extracellular, extrafibrillar phase ('ground substance') was extracted with isotonic saline, the cells were dispersed by treatment with trypsin, and the collagen was quantitatively converted to gelatin by autoclaving, leaving a negligible residue. The optimal conditions for each step were ascertained and the compositions of the fractions were examined. An appendix describes a device for the extraction of multiple tissue samples.","PeriodicalId":9531,"journal":{"name":"Canadian journal of biochemistry and physiology","volume":"11 1","pages":"2307-26"},"PeriodicalIF":0.0,"publicationDate":"1963-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82669284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The effects of various N-alkylated derivatives of aspartic acid on the synthesis of urea by rat liver homogenates have been studied. At 5 × 10−3 M concentration, N-methyl, N-ethyl, N-isopropyl, and N-cyclohexyl aspartic acids are not utilized and have no effect on the formation of urea. At this concentration, N-allyl-DL-aspartic acid inhibits the formation of endogenous urea by 77%. At concentrations of 2.5 to 7.5 × 10−2 M, N-methyl, N-ethyl, and N-isopropyl aspartic acids slightly increase the formation of endogenous urea; this is about 15% of the value obtained when aspartic acid alone is added at the same concentration. In the case of simple N-alkylated aspartic acids, liver homogenates are able to cleave the alkylated chain with the result that a small amount of urea synthesis is possible. N-allylaspartic acid totally inhibits the formation of urea from aspartic acid at a relatively low concentration of 6.2 × 10−3 M. N-cyclohexylaspartic acid has also an inhibitory effect which is ten times less prono...
{"title":"FORMATION OF UREA IN LIVER HOMOGENATES FROM CITRULLINE AND VARIOUS N-ALKYL DERIVATIVES OF ASPARTIC ACID.","authors":"R. Charbonneau, L. Berlinguet","doi":"10.1139/Y63-259","DOIUrl":"https://doi.org/10.1139/Y63-259","url":null,"abstract":"The effects of various N-alkylated derivatives of aspartic acid on the synthesis of urea by rat liver homogenates have been studied. At 5 × 10−3 M concentration, N-methyl, N-ethyl, N-isopropyl, and N-cyclohexyl aspartic acids are not utilized and have no effect on the formation of urea. At this concentration, N-allyl-DL-aspartic acid inhibits the formation of endogenous urea by 77%. At concentrations of 2.5 to 7.5 × 10−2 M, N-methyl, N-ethyl, and N-isopropyl aspartic acids slightly increase the formation of endogenous urea; this is about 15% of the value obtained when aspartic acid alone is added at the same concentration. In the case of simple N-alkylated aspartic acids, liver homogenates are able to cleave the alkylated chain with the result that a small amount of urea synthesis is possible. N-allylaspartic acid totally inhibits the formation of urea from aspartic acid at a relatively low concentration of 6.2 × 10−3 M. N-cyclohexylaspartic acid has also an inhibitory effect which is ten times less prono...","PeriodicalId":9531,"journal":{"name":"Canadian journal of biochemistry and physiology","volume":"67 1","pages":"2297-305"},"PeriodicalIF":0.0,"publicationDate":"1963-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76519812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"RESPIRATORY QUOTIENT OF THE RAT DIAPHRAGM.","authors":"A K SUSHEELA, J C GEORGE","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":9531,"journal":{"name":"Canadian journal of biochemistry and physiology","volume":"41 ","pages":"2221-3"},"PeriodicalIF":0.0,"publicationDate":"1963-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23672564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"THE EFFECT OF SOME TRANSPLANTABLE TUMORS ON THE NUCLEIC ACID METABOLISM OF THE HOST TISSUES.","authors":"J C NIXON, S H ZBARSKY","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":9531,"journal":{"name":"Canadian journal of biochemistry and physiology","volume":"41 ","pages":"2237-47"},"PeriodicalIF":0.0,"publicationDate":"1963-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23672566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"INFLUENCE OF DIET COMPOSITION AND CALORIC INTAKE ON BODY WEIGHT OF WARM- AND COLD-ACCLIMATED RATS.","authors":"A DESMARAIS, P A LACHANCE","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":9531,"journal":{"name":"Canadian journal of biochemistry and physiology","volume":"41 ","pages":"2225-35"},"PeriodicalIF":0.0,"publicationDate":"1963-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23672565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"USE OF I-125-LABELED 5-IODO-2'-DEOXYURIDINE FOR THE MEASUREMENT OF DNA SYNTHESIS IN MAMMALIAN CELLS IN VITRO.","authors":"S MAK, J E TILL","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":9531,"journal":{"name":"Canadian journal of biochemistry and physiology","volume":"41 ","pages":"2343-51"},"PeriodicalIF":0.0,"publicationDate":"1963-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23672573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"SIMULTANEOUS DETERMINATION OF KREBS CYCLE ACIDS BY GAS-LIQUID CHROMATOGRAPHY.","authors":"A KUKSIS, P VISHWAKARMA","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":9531,"journal":{"name":"Canadian journal of biochemistry and physiology","volume":"41 ","pages":"2353-62"},"PeriodicalIF":0.0,"publicationDate":"1963-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23672574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L BERLINGUET, L M BABINEAU, N BEGIN, J DUNNIGAN, F MARTEL
{"title":"PRELIMINARY BIOCHEMICAL STUDIES OF SOME EPSILON-SUBSTITUTED LYSINES.","authors":"L BERLINGUET, L M BABINEAU, N BEGIN, J DUNNIGAN, F MARTEL","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":9531,"journal":{"name":"Canadian journal of biochemistry and physiology","volume":"41 ","pages":"2373-5"},"PeriodicalIF":0.0,"publicationDate":"1963-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23672576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"INHIBITORY EFFECTS OF DECARBOXYLASE INHIBITORS ON THE METABOLISM OF TYROSINE-C14 IN THE RAT.","authors":"J F MORAN, T L SOURKES, B CHAVEZ","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":9531,"journal":{"name":"Canadian journal of biochemistry and physiology","volume":"41 ","pages":"2376-9"},"PeriodicalIF":0.0,"publicationDate":"1963-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23672577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A study was made of the effect of several transplantable tumors on the incorporation in vivo of formate-C14 and tritiated thymidine into the nucleic acids of the host tissues. The presence of the a...
本文研究了几种可移植肿瘤对体内甲酸- c14和氚化胸腺嘧啶进入宿主组织核酸的影响。a的存在…
{"title":"THE EFFECT OF SOME TRANSPLANTABLE TUMORS ON THE NUCLEIC ACID METABOLISM OF THE HOST TISSUES.","authors":"J. C. Nixon, S. H. Zbarsky","doi":"10.1139/O63-253","DOIUrl":"https://doi.org/10.1139/O63-253","url":null,"abstract":"A study was made of the effect of several transplantable tumors on the incorporation in vivo of formate-C14 and tritiated thymidine into the nucleic acids of the host tissues. The presence of the a...","PeriodicalId":9531,"journal":{"name":"Canadian journal of biochemistry and physiology","volume":"23 1","pages":"2237-47"},"PeriodicalIF":0.0,"publicationDate":"1963-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74628733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：