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IF 4 2区 生物学 Q2 CELL BIOLOGY
Cell calcium
Pub Date : 2025-01-01
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引用次数: 0
IF 4 2区 生物学 Q2 CELL BIOLOGY
Cell calcium
Pub Date : 2025-01-01
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引用次数: 0
IF 4 2区 生物学 Q2 CELL BIOLOGY
Cell calcium
Pub Date : 2025-01-01
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引用次数: 0
Unlikely allies: Cholesterol, calcium, and cytokines drive neutrophil activation in Behcet's disease 不可能的盟友：胆固醇，钙和细胞因子驱动中性粒细胞活化在白塞病。
IF 4.3 2区 生物学 Q2 CELL BIOLOGY
Cell calcium
Pub Date : 2025-01-01 DOI: 10.1016/j.ceca.2024.102991
Atif Towheed , Daniella M. Schwartz
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引用次数: 0
IF 4 2区 生物学 Q2 CELL BIOLOGY
Cell calcium
Pub Date : 2025-01-01
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引用次数: 0
IF 4 2区 生物学 Q2 CELL BIOLOGY
Cell calcium
Pub Date : 2025-01-01
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引用次数: 0
All-optical mapping of Ca2+ transport and homeostasis in dendrites 钙离子在树突中的运输和稳态的全光学定位。
IF 4.3 2区 生物学 Q2 CELL BIOLOGY
Cell calcium
Pub Date : 2025-01-01 DOI: 10.1016/j.ceca.2024.102983
Rebecca Frank Hayward , Adam E. Cohen
Calcium mediates many important signals in dendrites. However, the basic transport properties of calcium in dendrites have been difficult to measure: how far and how fast does a local influx of calcium propagate? We developed an all-optical system for simultaneous targeted Ca2+ import and Ca2+ concentration mapping. We co-expressed a blue light-activated calcium selective channelrhodopsin, CapChR2, with a far-red calcium sensor, FR-GECO1c, in cultured rat hippocampal neurons, and used patterned optogenetic stimulation to introduce calcium into cells with user-defined patterns of space and time. We determined a mean steady-state length constant for Ca2+ transport ϕ ∼ 5.8 μm, a half-life for return to baseline t1/2 ∼ 1.7 s, and an effective diffusion coefficient D ∼ 20 μm2/s, though there were substantial differences in Ca2+ dynamics between proximal and distal dendrites. At high Ca2+ concentration, distal dendrites showed nonlinear activation of Ca2+ efflux, which we pharmacologically ascribed to the NCX1 antiporter. Genetically encoded tools for all-optical mapping of Ca2+ transport and handling provide a powerful capability for studying this important messenger.
钙在树突中介导许多重要信号。然而，钙在树突中的基本运输特性一直难以测量：局部钙流入的传播距离和速度有多快？我们开发了一种全光学系统，用于同时靶向Ca2+导入和Ca2+浓度制图。我们在培养的大鼠海马神经元中共同表达蓝光激活的钙选择性通道视紫红质CapChR2和远红色钙传感器FR-GECO1c，并使用图案光遗传刺激将钙引入具有用户定义的空间和时间模式的细胞中。我们确定了Ca2+传输φ ~ 5.8 μm的平均稳态长度常数，返回基线的半衰期t1/2 ~ 1.7 s，有效扩散系数D ~ 20 μm2/s，尽管近端和远端树突之间Ca2+动力学存在实质性差异。在高Ca2+浓度下，远端树突表现出Ca2+外排的非线性激活，我们在药理学上将其归因于NCX1反转运蛋白。基因编码工具用于Ca2+运输和处理的全光学制图为研究这一重要信使提供了强大的能力。
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引用次数: 0
IF 4 2区 生物学 Q2 CELL BIOLOGY
Cell calcium
Pub Date : 2025-01-01
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引用次数: 0
Corrigendum to “Modulating TRPV4 channels with paclitaxel and lithium” [Cell Calcium 91 (2020) 102266] “用紫杉醇和锂调制TRPV4通道”的勘误表[Cell Calcium 91(2020) 102266]。
IF 4.3 2区 生物学 Q2 CELL BIOLOGY
Cell calcium
Pub Date : 2025-01-01 DOI: 10.1016/j.ceca.2024.102988
Julio C. Sánchez , Laura V. Muñoz , Barbara E. Ehrlich
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引用次数: 0
Regulation of SR and mitochondrial Ca2+ signaling by L-type Ca2+ channels and Na/Ca exchanger in hiPSC–CMs l型Ca2+通道和Na/Ca交换器对hiPSC-CMs中SR和线粒体Ca2+信号的调控
IF 4.3 2区 生物学 Q2 CELL BIOLOGY
Cell calcium
Pub Date : 2025-01-01 DOI: 10.1016/j.ceca.2024.102985
Xiao-Hua Zhang, Martin Morad

Rationale & methods

While signaling of cardiac SR by surface membrane proteins (ICa & INCX) is well studied, the regulation of mitochondrial Ca2+ by plasmalemmal proteins remains less explored. Here we have examined the signaling of mitochondria and SR by surface-membrane calcium-transporting proteins, using genetically engineered targeted fluorescent probes, mito-GCamP6 and R-CEPIA1er.

Results

In voltage-clamped and TIRF-imaged cardiomyocytes, low Na+ induced SR Ca2+ release was suppressed by short pre-exposures to ∼100 nM FCCP, suggesting mitochondrial Ca2+ contribution to low Na+ triggered SR Ca2+release. Even though low Na+- or caffeine-triggered SR Ca2+ release activated global mitochondrial Ca2+ uptake, focal mitochondrial Ca2+ signals varied in kinetics and magnitude, showing uptake or release of calcium, depending on cellular location of mitochondria. In spontaneously pacing cells, sustained caffeine exposures depleted the SR Ca2+ content activating mitochondrial Ca2+ uptake followed by sustained mitochondrial pacing. Spontaneous hiPSCCMs pacing was strongly suppressed by L-type calcium channels blockers, but not by inhibiting SERCA2a by CPA.

Conclusion

Spontaneous hiPSCCMs pacing is triggered by influx of calcium through L-type Ca2+ channel that gates the release of SR pools supplemented by NCX-mediated mitochondrial calcium contribution.
原理和方法：虽然表面膜蛋白（ICa和INCX）对心脏SR的信号传导已经得到了很好的研究，但质乳蛋白对线粒体Ca2+的调节仍然很少被探索。在这里，我们使用基因工程靶向荧光探针，mito-GCamP6和R-CEPIA1er，研究了表面膜钙转运蛋白对线粒体和SR的信号传导。结果：在电压箝位和tirf成像的心肌细胞中，低Na+诱导的SR Ca2+释放被短时间暴露于~ 100 nM FCCP抑制，这表明线粒体Ca2+对低Na+触发的SR Ca2+释放有贡献。即使低Na+或咖啡因触发的SR Ca2+释放激活了线粒体Ca2+的整体摄取，局点线粒体Ca2+信号在动力学和大小上变化，显示钙的摄取或释放，取决于线粒体的细胞位置。在自发起搏细胞中，持续的咖啡因暴露耗尽了SR Ca2+含量，激活了线粒体Ca2+摄取，随后是持续的线粒体起搏。l型钙通道阻滞剂能强烈抑制hipsccm自发性起搏，但CPA不能抑制SERCA2a。结论：自发的hiPSCCMs起搏是由钙通过l型Ca2+通道流入触发的，该通道抑制了SR池的释放，并补充了ncx介导的线粒体钙贡献。
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