Cell and Tissue Banking最新文献

To summarize the evidence examining the outcomes of Descemet membrane endothelial keratoplasty (DMEK) using eye bank pre-stripped versus surgeon prepared grafts. Systematic review and meta-analysis. This study was conducted following the preferred reporting items for systematic reviews and meta-analyses consensus statement (PROSPERO ID: CRD42023457120). Searches of medline (Ovid), EMBASE, EMCARE, the Cochrane register of controlled trials, and grey literature were performed until April 2025. All comparative studies comparing DMEK outcomes after eye bank prepared (pre-stripped only) with surgeon-prepared tissue were included. Two independent reviewers completed data extraction and performed quality assessments. The primary outcomes were the rebubbling rate and corrected distance visual acuity (CDVA). Results were summarized using a random-effects meta-analysis. Five studies totalling 750 eyes receiving DMEK grafts were included. Four were retrospective observational studies and one was a non-randomized prospective study. There were no significant differences between eye bank pre-stripped and surgeon-prepared graft outcomes with regards to rebubbling rate (odds ratio, 1.11; 95% confidence interval [CI] 0.65 to 1.90) and postoperative logMAR CDVA (mean difference - 0.11, 95% CI - 0.23 to 0.01). No statistically significant difference was noted in postoperative corneal thickness, endothelial cell loss or density, and postoperative complications.Eye bank pre-stripped grafts and surgeon-prepared grafts yield comparable outcomes in terms of CDVA and rebubbling rates following DMEK.However, there is limited evidence, as only five studies were included in this analysis.

To evaluate long‑term outcomes of corneal patch grafting (CPG) and to determine prognostic factors for anatomical and functional success. This retrospective study included 35 eyes from 35 patients who underwent CPG between April 2016 and September 2022 at Adana City Training and Research Hospital. Collected data included age, sex, preoperative and postoperative best-corrected visual acuity (BCVA), graft localization and size, anterior segment findings, graft survival, secondary surgical procedures, and rates of anatomical and functional success. Anatomical success was achieved in 82.9% (29/35), and functional success in 45.7% (16/35). The most common reason for CPG was non-infectious, non-traumatic perforation related to immune disorders (37.1%). Anatomical success was not significantly associated with the primary etiology (p = 0.73), whereas functional success was significantly higher in patients with traumatic corneal perforations (p = 0.029). A statistically significant improvement was observed in postoperative BCVA for the study group (p < 0.001). Functional success was significantly associated with younger age and peripheral graft location (p = 0.005 and p < 0.001, respectively). Smaller graft diameter was significantly correlated with both higher anatomical and functional success (p = 0.031 and p = 0.007, respectively), while no significant association was found with horizontal graft diameter (p = 0.27 and p = 0.068, respectively). Corneal patch grafting is a highly effective technique for restoring anatomical integrity in most patients and provides acceptable functional outcomes in selected cases. Younger age, peripheral graft location, and smaller graft diameter appear to be favorable prognostic factors for functional recovery.

In the United States, the use of Food & Drug Administration (FDA)-licensed, approved, or cleared tests is required for infectious disease screening and determining the eligibility of deceased donors for all Human Cells, Tissues, and Cellular and Tissue-Based Products (HCT/Ps). With the discontinuation of two manual enzyme-linked immunoassay (EIA) tests, automated Chemiluminescent Microparticle Immunoassay (CMIA) technology was introduced as the primary alternative. This study compares serologic reactivity rates between manual EIA and automated CMIA methods. Serology testing was performed on blood specimens from deceased tissue and cornea donors using either manual EIA assays or automated CMIA platforms. A retrospective analysis of over 160,000 donors was conducted to evaluate assay-specific reactive rates. Concordance between serology and Nucleic Acid Testing (NAT) results was also assessed. Reactive rates for HBsAg, HIV-1/2 antibodies, and HTLV-I/II antibodies increased following the transition to CMIA-based testing compared to manual EIA methods. However, these increases were not associated with a corresponding rise in NAT reactive results, indicating a potential increase in false-positive or non-viremic results. Automated testing offers improved quality control and reduces variability associated with manual techniques. However, the shift to CMIA assays resulted in a higher number of reactive serology results, leading to increased donor ineligibility despite negative NAT results. The data presented here highlights the potential impact on donor deferral rates for tissue and cornea donors due to assay platform changes.

Although many preclinical and clinical studies are ongoing on amniotic membrane extract (AME), an amniotic membrane-derived product developed to support ocular surface healing, the effect of AME on the basic cellular functions and properties of human corneal epithelial cells (hCECs) has not been clearly defined. In this study, we aimed to evaluate the effect of AME supplementation to the culture media, on basic cellular functions of hCECs and on expression of specific cell markers of hCECs, as well as to determine its effectiveness in an experimental in vitro wound model. hCECs were seeded with the constant cell density in 6, 24 and 48 well plates. The next day, the media was refreshed with 0 mg/ml, 0.75 mg/ml, 1.5 mg/ml and 3 mg/ml final concentration of AME supplemented complete growth medium. Cellular morphology, viability, metabolic activity, proliferation assessments and immunocytochemistry were conducted on the time points at day 3, 6 and 9. Then, in vitro wound healing assay was performed on hCECs under the effect of AME. AME did not affect cellular morphology, viability, metabolic activity and proliferation. AME supplementation induced CK3 and CK12 expression of hCECs significantly (p < 0.05) higher than without AME group. In vitro wound healing assay revealed that while control and AME-treated cultures both exhibited healing, AME-treated mechanical abrasions closed at a greater rate compared to control. Our study shows that AME promotes in vitro wound healing and cell characteristics in terms of CK3 and CK12 protein expressions, while preserving basic cellular functions of corneal epithelial cells.

Human Tissue Banks (BTH) must validate the storage of collected/processed tissues ensuring physical integrity, sterility, and microbial protection for up to 5 years. (1) Is it safe to use bone tissue for transplants collected by a BTH after 5 years of storage? (2) Do the packaging of stored tissues present physical integrity, sterility, and microbial protection after 5 years? (3) What are the morphological results of bone tissues after 5 years?. 20 femoral heads were used with a storage time of between 9 and 10 years at -80 °C. From each femoral head, the following were carried out: microbiological tests for aerobic and anaerobic bacteria and fungi, collecting 3 fragments of bone tissue, 3 Stuart swabs from the inner surface of the packaging, and a sample of 0.9% SF for Bioburden examination; tissue histology; the quantitative and qualitative mechanical resistance test and pyrogenicity/cytotoxicity test were used on the packaging, pre and post storage. No bone tissue samples showed pathogenicity. Histological findings showed morphologically preserved osteocytes with points of bone degeneration and necrotic adipose tissue. No packaging showed contamination, cytotoxicity, or pyrogenicity. The mechanical properties of these packages demonstrated uniformity in thickness, high tension, and relative stiffness even after storage (p = 0.001). It is concluded that the packaging used in the study presented physical integrity, sterility, and microbial protection for bone tissues after 9 and 10 years of storage. A possible increase in the shelf life of fabrics is contemplated to up to 10 years. Such results expand future research directions to continuously improve the quality of products and services offered by BTH.

Prosthetic valves derived from bovine pericardium (BP) are crucial for heart valve replacement, yet current crosslinking methods with glutaraldehyde can lead to immune responses and calcification. This study evaluated the effects of reducing the glutaraldehyde crosslinking time from 10 to 5 days in bovine pericardial patches for use as heart valve substitutes. In addition to examining the physical properties of the BP, the study analyzed the biocompatibility, tissue structure, and calcification of the pericardial tissue. BPs were processed using two protocols based on the fixation time with glutaraldehyde: BP10d (10 days) and BP5d (5 days). All samples were treated with glutamic acid to neutralize residual aldehyde groups from the glutaraldehyde. Subsequently, the resulting material was assessed for mechanical and thermal properties and histologically using light and scanning electron microscopy. Post-implantation histological evaluation and calcium content determination were conducted after 7, 14, 30, 60 and 120 days. The calcification was a rare occurrence. However, some samples from the BP10d group displayed positive Von Kossa staining, indicating mineral deposition. Chemical analysis using ICP-OES revealed low calcium concentrations in the explants of both groups, with higher concentrations observed in the BP10d group during the later analysis periods. Mechanical and thermal stability assessments showed no significant differences between experimental groups. Histological examination revealed more collagen and elastic fibers deformation, and inflammation in the BP10d group compared to the BP5d group. The revised manufacturing protocol, with a 5-day fixation time, showed promising anti-calcifying activity, biocompatibility, and tissue preservation.

Sinus lift surgery is essential after pneumatization caused by loss of posterior teeth. Leukocyte-Platelet-Rich Fibrin (L-PRF) accelerates bone healing by releasing growth factors that promote angiogenesis, cell differentiation, and inflammatory modulation. To evaluate the efficacy of L-PRF in bone healing and repair in sinus lift surgeries, in addition to investigating its role in angiogenesis and inflammatory modulation. The systematic review protocol included definition of the research question, search strategy, inclusion and exclusion criteria, study types, effect measures, screening methods, and data analysis. The search resulted in 860 studies. After removal of duplicates, 704 articles remained, of which 11 met the inclusion criteria. After careful evaluation, 4 studies were considered highly relevant and included in the systematic review. Evidence indicates that the combination of L-PRF with bone grafts, such as DBBM, can accelerate bone formation and allow early implant placement, supported by increased expression of protein markers essential for osteogenesis. The addition of L-PRF to DBBM demonstrated significant benefits in promoting a more favorable bone environment, reducing the time required for osseointegration.

The body has evolved three types of cartilage: hyaline, elastic, and fibrocartilage. Modern tissue engineering techniques can harvest different types of chondrocytes, expand them in vitro, and use them to repair various cartilage defects. However, the modulatory effect of different cartilaginous niches on the type of regenerated cartilage after the implantation of chondrocytes from different origins remains unknown. In this study, three typical types of cartilage-auricular (elastic cartilage), articular (hyaline cartilage), and meniscus (fibrocartilage)-were investigated. Chondrocytes derived from these cartilages were mixed with Pluronic gel and implanted into three different cartilaginous niches for one month using a goat model. Our results demonstrated that in the articular cartilage environment, regenerated cartilage from auricular chondrocytes lost elastin expression, and cartilage from meniscus chondrocytes lacked a fibrous structure, showing reduced type I collagen and increased type II collagen expression, all resembling a hyaline cartilage-like structure. In the auricular cartilage environment, regenerated cartilage from articular chondrocytes did not express elastin, maintaining a hyaline cartilage-like structure, while fibrocartilage chondrocytes failed to form regenerated cartilage. In the fibrocartilage environment, regenerated cartilage from auricular and meniscus chondrocytes did not exhibit a fibrous structure, with weak type I collagen expression and positive type II collagen expression. Regenerated cartilage from auricular chondrocytes did not express elastin and did not transform into fibrocartilage. This study provides valuable insights into how different cartilaginous niches influence the characteristics of regenerated cartilage, offering potential implications for improving cartilage repair strategies in tissue engineering.

The study explores the effects of the COVID-19 pandemic on the Musculoskeletal Tissue Bank (MSTB) in Milan, with a particular focus on tissue harvesting and its subsequent use in surgical procedures. A retrospective descriptive epidemiological analysis compared data from the pre-pandemic period (2018-2019) with that of the pandemic period (2020-2022), revealing a 24.8% reduction in tissue retrievals during the pandemic. Although there was a decrease in the number of eligible donors not collected (from 93 to 67, from 36.05 to 34.54%), this reduction was not statistically significant. The decline in tissue retrievals was due to decreased non-COVID-related pathologies, a lower number of potential donors from reduced accidents and increase in COVID-positive deaths. However, the MSTB successfully met tissue demands throughout this period. Notably, the reduction in retrievals at the MSTB was lower than national averages (- 24.8 vs. - 47.5%). Logistic regression analysis showed no significant organizational issues in donor collection. Despite the challenges, the MSTB remained resilient and adaptable, continuing its essential services. This underscores the broader impact of the pandemic on healthcare systems and emphasizes the importance of a flexible healthcare infrastructure during public health emergencies.

A prospective clinical study was conducted to evaluate the change in horizontal alveolar ridge width following horizontal ridge augmentation using Freeze-Dried Bone Allograft (FDBA) block graft and Amnion Chorion Membrane (ACM) for the management of atrophic ridges. The study included a total of 10 subjects of either sex, aged between 18 and 60 years, presenting with deficient residual alveolar ridge width. All subjects were treated with horizontal ridge augmentation using an indigenously prepared FDBA block graft and ACM. The ridge width at the alveolar crest and 5 mm apical to the crest were recorded at baseline, immediately after augmentation, and after 6 months, followed by dental implant placement. Also, this study focused on the clinical efficacy and any adverse effects of the indigenously prepared FDBA block graft and ACM. The indigenously prepared FDBA block graft and ACM exhibit regenerative properties that significantly enhance horizontal alveolar ridge width in all cases of deficient ridges, thereby greatly facilitating dental implant placement. The mean difference after 6 months at the crest was 2.21 ± 0.89 mm (p < 0.05) and at 5 mm apical to the crest was 2.8 ± 0.91 mm (p = 0.001)This significant increase in ridge width achieved through indigenously prepared FDBA and ACM serves as an excellent alternative to commercially sourced or imported allograft materials, making it a cost-efficient choice.