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Potential of human amniotic membrane application for articular cartilage regeneration: a review. 人羊膜在关节软骨再生中的应用潜力综述。
IF 2 4区 医学 Q4 CELL BIOLOGY Pub Date : 2025-11-24 DOI: 10.1007/s10561-025-10202-2
Shadi Askari, Elahe Entekhabi, Arezoo Firouzeh, Aylar Eslami Saed, Masoumeh Haghbin Nazarpak

Regeneration of articular cartilage disorders is one of the critical challenges in musculoskeletal medicine. Tissue engineering could represent a therapeutic option to support cartilage regeneration. Natural and biological materials are appropriate for fabricating tissue engineering scaffolds because of their similarity to natural tissues. The properties of amniotic membranes, including low immunogenicity, anti-inflammatory role, cell loading capability, expression of various growth factors, and chondroprotective effect, make them an interesting option for cartilage regeneration. This review studied the structure of articular cartilage and potential applications of the human amniotic membrane (AM) for articular cartilage regeneration. In addition, processing and decellularization methods of AM and the most common forms of amniotic membrane used in cartilage regeneration, including sheet, injectable form, and 3D forms, were studied. This review highlights the benefits of amniotic membrane applications in cartilage regeneration and clinical trial studies.

关节软骨疾病的再生是肌肉骨骼医学的关键挑战之一。组织工程可能是支持软骨再生的一种治疗选择。天然材料和生物材料因其与天然组织的相似性而适合用于组织工程支架的制造。羊膜的特性,包括低免疫原性、抗炎作用、细胞负荷能力、各种生长因子的表达和软骨保护作用,使其成为软骨再生的一个有趣的选择。本文综述了人羊膜在关节软骨再生中的应用前景及关节软骨的结构。此外,研究了AM和用于软骨再生的最常见形式的羊膜的加工和脱细胞方法,包括片状,注射形式和3D形式。本文综述了羊膜在软骨再生和临床试验研究中的应用。
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引用次数: 0
Does platelet lysate switch cryopreserved human tissues on? 血小板裂解液能开启人体冷冻组织吗?
IF 2 4区 医学 Q4 CELL BIOLOGY Pub Date : 2025-11-04 DOI: 10.1007/s10561-025-10199-8
Sara Mirabet, Néstor Villalba, Vicente Mirabet, Luis Larrea, Carmen Escobedo-Lucea

Platelet lysate is a derivative of platelet-rich plasma that is used as supplement for in vitro cell culture media. A variety of protocols for its preparation have been described. However, its potential use in the clinical setting has been poorly studied. In the present work, the effect of several protocols on cell proliferation has been comparatively analyzed. Additionally, the effect of exposing bone and vascular tissues to different concentrations of platelet lysate has also been analyzed. Human fibroblast-like cells were used to test preparation protocols. Thawed skull and artery fragments were incubated with platelet lysate and seeded as explants in culture plates. Cell growth was evaluated quantitatively in the first assay (cell count) and qualitatively in the second (presence of growing cell colonies). The presence of leukocytes in the raw material to obtain the platelet lysate was correlated with higher cell proliferation. In all cultures from arteries and 71.4% of those from bones, the presence of viable cells was detected. No statistically significant differences that correlated with the percentage of platelet lysate used during the post-thaw incubation were observed. The main findings of this study revealed that: there is a contribution of bioactive substances for cell growth by lymphocytes, incubation with platelet lysate had no significant activating effect on cells in thawed tissues, arteries stored in liquid nitrogen retained cell viability for long periods (over 5 years), and cell viability in bones stored at - 80 °C decreased after 3 months.

血小板裂解液是富血小板血浆的衍生物,用作体外细胞培养基的补充。介绍了其制备的各种方案。然而,其在临床环境中的潜在应用尚未得到充分研究。在本工作中,比较分析了几种方案对细胞增殖的影响。此外,还分析了将骨和血管组织暴露于不同浓度的血小板裂解液的影响。人类成纤维细胞样细胞用于测试制备方案。解冻后的颅骨和动脉碎片与血小板裂解液孵育,作为外植体植入培养板。细胞生长在第一次测定中进行定量评估(细胞计数),在第二次测定中进行定性评估(生长细胞菌落的存在)。在获得血小板裂解液的原料中存在白细胞与较高的细胞增殖有关。在所有动脉培养物和71.4%的骨骼培养物中,都检测到活细胞的存在。未观察到与解冻后孵育期间使用的血小板裂解液百分比相关的统计学显著差异。本研究的主要发现是:淋巴细胞对细胞生长有生物活性物质的贡献,血小板裂解液对解冻组织细胞无明显的活化作用,液氮保存的动脉可长时间(5年以上)保持细胞活力,- 80℃保存3个月后骨骼细胞活力下降。
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引用次数: 0
Comparing the efficacy of two antibiotic cocktails in decontamination of cardiovascular tissues. 两种抗生素鸡尾酒对心血管组织去污效果的比较。
IF 2 4区 医学 Q4 CELL BIOLOGY Pub Date : 2025-10-30 DOI: 10.1007/s10561-025-10198-9
Alina Levy, Helit Cohen, Nadezda Savieva, Meytal Neeman-Azulay, Natasha Belausov, Ehud Raanani, David Mishali, Rachel Kornhaber, Michelle Cleary, Jonathan Esensten, Sharon Amit, Ayelet Di Segni

Cardiovascular allografts are essential for patients with severe cardiovascular diseases. Yet, microbial contamination of the grafts poses a life-threatening risk to recipients. Tissue banks utilize various decontamination methods during cardiovascular tissue processing, often involving antibiotic solutions. This study compares the efficacy of an in-house prepared antibiotic cocktail (tissue bank cocktail) and a commercially available solution (BASE.128) in decontaminating cardiovascular tissues. For this study, the efficacy of the two antibiotic cocktails was compared through quantitative comparisons against challenge microorganisms, and retrospective analysis of routine sterility tests. Both solutions demonstrated comparable decontamination efficiency against challenge strains, achieving significant reductions in bacterial load. However, retrospective sterility tests revealed that while both antibiotic solutions were highly effective in decontaminating cardiovascular allografts, the use of BASE.128 followed a tenfold increase in contamination rates compared to the tissue bank cocktail, primarily due to a slow-growing non-tuberculous mycobacteria strain. These findings highlight the importance of tailored decontamination protocols that consider prevalent microbial contaminants while preserving tissue quality.

心血管异体移植对于严重心血管疾病患者是必不可少的。然而,移植物的微生物污染对受者构成威胁生命的危险。组织库在心血管组织处理过程中使用各种去污方法,通常涉及抗生素溶液。本研究比较了内部制备的抗生素鸡尾酒(组织库鸡尾酒)和市售溶液(BASE.128)在净化心血管组织方面的效果。在本研究中,通过对攻毒微生物的定量比较和常规无菌检查的回顾性分析,比较了两种抗生素鸡尾酒的疗效。两种解决方案对挑战菌株表现出相当的去污效率,实现了细菌负荷的显著减少。然而,回顾性无菌试验显示,虽然这两种抗生素溶液对去污心血管同种异体移植物都非常有效,但与组织库鸡尾酒相比,使用BASE.128的污染率增加了10倍,主要是由于生长缓慢的非结核分枝杆菌菌株。这些发现强调了在保持组织质量的同时考虑普遍存在的微生物污染物的定制去污方案的重要性。
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引用次数: 0
Optimizing a feasible protocol for acellular nerve allografts: An experimental study. 优化脱细胞神经同种异体移植的可行方案:一项实验研究。
IF 2 4区 医学 Q4 CELL BIOLOGY Pub Date : 2025-10-30 DOI: 10.1007/s10561-025-10189-w
Daniel Camporro Fernández, Marta de Juan Marín, Marta Pevida, Sara Llames, Juan Argüelles Luís, Álvaro Meana

Peripheral nerve injuries often require surgical intervention when end-to-end coaptation is not feasible, with autologous nerve grafts being the current gold standard. However, limitations such as donor-site defects drive the search for alternative methods. This study explores the efficacy of acellular nerve allografts obtained through a feasible protocol as a potential off-the-shelf substitute for autografting in a 14-mm rat sciatic nerve defect. Thirty-two female Wistar rats were divided into four groups: autograft, lyophilized acellular allograft, fresh acellular allograft and silicone tube. Functional assessments and histological examinations were performed at 14 and 20 weeks post-surgery, respectively. Results showed comparable axonal regeneration between acellular nerve allografts and autografts. Histomorphometric analysis revealed no significant differences in axonal characteristics between groups. Muscle histomorphometry indicated superior recovery in animals treated with fresh acellular allografts, who exhibited the least muscle atrophy and larger muscle fiber diameter compared to lyophilized processed allografts and autografts. Functional assessments revealed no significant intergroup differences. Processed acellular allografts promote axonal regeneration similar to autografts in a 14-mm rat sciatic nerve defect. Fresh acellular allografts achieve better muscle reinnervation in the medial gastrocnemius muscle. However, axonal regeneration does not consistently correlate with functional or histomorphological outcomes of the hind leg muscle. The successful decellularization protocol and lack of immune rejection pave the way for adapting it to human nerve grafts. These could revolutionize clinical practice in our country, becoming an example of leveraging existing resources and replacing collagen conduits and autografts for treating certain injuries.

当端到端适应不可行时,周围神经损伤通常需要手术干预,自体神经移植是目前的金标准。然而,诸如供体部位缺陷之类的限制促使人们寻找替代方法。本研究探讨了通过可行方案获得的脱细胞神经同种异体移植物作为自体移植物在14mm大鼠坐骨神经缺损中潜在的现成替代品的疗效。32只雌性Wistar大鼠分为自体移植物组、冻干脱细胞异体移植物组、新鲜脱细胞异体移植物组和硅胶管组。术后14周和20周分别进行功能评估和组织学检查。结果显示脱细胞神经异体移植和自体神经移植之间的轴突再生具有可比性。组织形态学分析显示各组间轴突特征无显著差异。肌肉组织形态测量显示,接受新鲜脱细胞同种异体移植物治疗的动物恢复良好,与冻干处理同种异体移植物和自体移植物相比,它们表现出最小的肌肉萎缩和更大的肌纤维直径。功能评估显示各组间无显著差异。处理的脱细胞异体移植物促进轴突再生类似于自体移植物在14mm大鼠坐骨神经缺损。新鲜的脱细胞异体移植物在腓肠肌内侧获得更好的肌肉再神经支配。然而,轴突再生并不总是与后腿肌肉的功能或组织形态学结果相关。成功的脱细胞方案和缺乏免疫排斥为适应人类神经移植铺平了道路。这可能会彻底改变我国的临床实践,成为利用现有资源和替代胶原蛋白导管和自体移植物治疗某些损伤的一个例子。
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引用次数: 0
In vitro evaluation of the nanofibers developed for peripheral nerve regeneration. 外周神经再生纳米纤维的体外评价。
IF 2 4区 医学 Q4 CELL BIOLOGY Pub Date : 2025-10-15 DOI: 10.1007/s10561-025-10201-3
Saandia Bacar, Suray Pehlivanoğlu, Emine Güler Akgemci

In tissue engineering, natural and synthetic nanofibers that can regenerate body damage have been successfully used in the repair of many lesion types, including peripheral neural lesions, in recent years. So, we developed three different nanofibers that we think can regenerate peripheral nerve damage. Three different nanofibers based on biodegradable poly-ε-caprolactone (PCL); Pure PCL (PCL) nanofiber, 70% PCL and 30% bioactive glass (PCL/BG) hybrid nanofiber, and 0.1% vitamin B12 added (PCL/BG)-B12 hybrid nanofiber were produced by electrospinning. Sol-gel method was used in the synthesis of biomaterials containing bioactive glass. The nanofibers were characterized by scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDX), and fourier transform infrared spectroscopy (FT-IR). Cell viability assays were performed with healthy L929 fibroblast cells and PC12 cells to evaluate the biocompatibility of nanofibers. Neuronal differentiation of PC12 cells were stimulated by nerve growth factor (NGF). To assess the differentiation levels of PC12 cells, the length of neurites and number of outgrowing neurites per cell was evaluated morphologically, and NGF production levels of the cells were determined by ELISA. The results suggest that these biocompatible nanofibers stimulated PC12 cell survival and neuronal differentiation. Among these scaffolds, PCL/BG-B12 nanofibers strikingly triggered NGF production of PC12 cells as a hallmark of neuroregeneration. Thus, the nanofibers are capable of neuroprotective properties due to their safe, supporting proliferation, and NGF-releasing capacity. Additionally, it could be suggested that the PCL/BG nanofiber and vitamin B12 have the potential to be used in further studies for neurodegenerative diseases.

在组织工程中,天然和合成纳米纤维可再生机体损伤,近年来已成功应用于多种损伤类型的修复,包括周围神经损伤。因此,我们开发了三种不同的纳米纤维,我们认为它们可以再生周围神经损伤。可生物降解聚ε-己内酯(PCL)纳米纤维的研究采用静电纺丝法制备了纯PCL (PCL)纳米纤维、70% PCL和30%生物活性玻璃(PCL/BG)混杂纳米纤维和添加0.1%维生素B12 (PCL/BG)-B12混杂纳米纤维。采用溶胶-凝胶法合成了含生物活性玻璃的生物材料。采用扫描电镜(SEM)、x射线能谱(EDX)和傅里叶变换红外光谱(FT-IR)对纳米纤维进行了表征。采用健康的L929成纤维细胞和PC12细胞进行细胞活力测定,评价纳米纤维的生物相容性。神经生长因子(NGF)刺激PC12细胞的神经元分化。为了评估PC12细胞的分化水平,形态学上评估了每个细胞的神经突长度和外生神经突数量,ELISA法检测了细胞的NGF生成水平。结果表明,这些生物相容性纳米纤维刺激了PC12细胞的存活和神经元分化。在这些支架中,PCL/BG-B12纳米纤维显著地触发了PC12细胞的NGF产生,这是神经再生的一个标志。因此,纳米纤维由于其安全、支持增殖和释放ngf的能力而具有神经保护特性。此外,这可能表明PCL/BG纳米纤维和维生素B12有潜力用于神经退行性疾病的进一步研究。
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引用次数: 0
Three-dimensionally decellularized human amniotic membrane scaffold: structure, processing, and biological properties. 三维脱细胞人羊膜支架:结构、加工和生物学特性。
IF 2 4区 医学 Q4 CELL BIOLOGY Pub Date : 2025-10-14 DOI: 10.1007/s10561-025-10196-x
Banafsheh Heidari, Soroush Shams, Nazanin Akbari, Kavosh Zandsalimi

Tissue engineering (TE) combines cells, biomaterials, and bioactive molecules to create functional tissue constructs aimed at restoring tissue function and improving patient outcomes. The human amniotic membrane (HAM) is a widely studied biological scaffold for various biomedical applications. Decellularization of HAM (dHAM) is necessary to reduce graft rejection but depletes stem cells and growth factors, potentially limiting regenerative potential. This study investigates the recellularization of dHAM with adipose-derived mesenchymal stem cells (AdMSCs) to enhance its bioactivity using a novel 3D seeding technique. Decellularized HAM (dHAM) was recellularized with AdMSCs employing a novel 3D seeding method to achieve uniform cell distribution within the scaffold. The viability, differentiation potential, and morphology of AdMSCs were assessed in both 2D and 3D culture systems. Flow cytometry was used to evaluate the differentiation capacity of AdMSCs into osteogenic, chondrogenic, and adipogenic lineages. Field emission scanning electron microscopy (FESEM) was utilized to analyze cell morphology and penetration depth within the scaffold. AdMSC viability was comparable between 2 and 3D cultures, indicating that dHAM scaffolds effectively support cell survival regardless of the culture technique. The composition and properties of dHAM preserved cell functions in both culture systems. Flow cytometry confirmed the multilineage differentiation potential of AdMSCs. FESEM imaging revealed AdMSCs with extending filopodia on the scaffold surface and cell penetration up to 17.68 µm into the dHAM matrix. The successful 3D recellularization of dHAM with AdMSCs demonstrates its potential as a biological scaffold for stem cell delivery. This approach holds promise for tissue repair and wound healing applications, enhancing the regenerative efficacy of dHAM-based constructs.

组织工程(TE)结合细胞、生物材料和生物活性分子来创建功能性组织结构,旨在恢复组织功能并改善患者预后。人羊膜(HAM)是一种被广泛研究的生物支架,具有广泛的生物医学应用。HAM的脱细胞化(dHAM)对于减少移植排斥是必要的,但会消耗干细胞和生长因子,潜在地限制再生潜力。本研究利用一种新的3D播种技术,研究了脂肪源性间充质干细胞(AdMSCs)对dHAM的再细胞化,以增强其生物活性。采用一种新颖的3D播种方法,用AdMSCs对脱细胞HAM (dHAM)进行再细胞化,以实现支架内细胞均匀分布。在二维和三维培养系统中评估AdMSCs的活力、分化潜力和形态。流式细胞术用于评估AdMSCs分化成成骨、软骨和脂肪谱系的能力。利用场发射扫描电镜(FESEM)分析支架内细胞形态和穿透深度。AdMSC的存活能力在2d和3D培养中是相当的,这表明无论培养技术如何,dHAM支架都能有效地支持细胞存活。dHAM的组成和性质在两种培养体系中保存了细胞功能。流式细胞术证实了AdMSCs的多系分化潜力。FESEM成像显示AdMSCs在支架表面具有延伸的丝状足,细胞渗透到dHAM基质中高达17.68µm。利用AdMSCs成功实现dHAM的3D再细胞化,证明了其作为干细胞递送生物支架的潜力。这种方法有望用于组织修复和伤口愈合应用,提高dham结构的再生功效。
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引用次数: 0
Deceased organ and tissue donor with burn injury. 因烧伤而死亡的器官和组织捐献者。
IF 2 4区 医学 Q4 CELL BIOLOGY Pub Date : 2025-10-13 DOI: 10.1007/s10561-025-10194-z
Wojciech Łabuś, Adam Kurek, Katarzyna Czerny, Karolina Ziółkowska, Marcin Gierek, Karolina Mikuś-Zagórska, Artur Wielgórecki, Anna Hepa-Banasik, Magdalena Szatan, Anna Słaboń, Agnieszka Klama-Baryła, Anna Sitkowska, Justyna Glik, Bartłomiej Nowak, Aleksandra Gil, Ryszard Maciejowski, Przemysław Strzelec, Artur Kamiński

The gap between the clinical demand for transplantable organs/tissues and the limited availability of deceased donors motivates the search for alternative donor types. This growing need has prompted exploration into non-traditional donor populations, including those who have died due to circulatory causes, elderly individuals, and donors previously excluded from consideration, such as those with extensive burns. Burned deceased donors are often perceived as unsuitable due to the detrimental effects of burn injury on multiple organ systems, including the phenomenon known as "burn disease." This condition, characterized by systemic inflammatory responses, metabolic derangements, and sepsis risk, can potentially compromise organ viability.However, proper assessment and management of these donors, including rigorous evaluation of organ function, the extent of burn injury, and potential infection, can enable successful multi-organ and multi-tissue donation. Several reports suggest that, under appropriate clinical and logistical circumstances, organs such as the kidneys, liver, heart, and lungs, as well as various tissues, can be procured from burned donors.Nonetheless, expanding research in this area is essential to establish the guidelines for safe utilization of burn deceased donor population.

可移植器官/组织的临床需求与有限的已故供体之间的差距促使人们寻找其他供体类型。这种日益增长的需求促使人们探索非传统的供体人群,包括那些因循环系统原因死亡的人、老年人和以前被排除在考虑之外的供体,例如那些大面积烧伤的人。由于烧伤对多个器官系统的有害影响,包括被称为“烧伤疾病”的现象,烧伤死者的供体通常被认为是不合适的。这种疾病以全身炎症反应、代谢紊乱和败血症风险为特征,可能会损害器官活力。然而,对这些供体进行适当的评估和管理,包括严格评估器官功能、烧伤程度和潜在感染,可以实现成功的多器官和多组织捐赠。一些报告表明,在适当的临床和后勤条件下,可以从烧伤供者处获得肾脏、肝脏、心脏和肺等器官以及各种组织。尽管如此,扩大这一领域的研究对于建立安全使用烧伤死亡供体人群的指导方针至关重要。
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引用次数: 0
Synthesis and characterization of silver nanoparticle-loaded carboxymethylcellulose hydrogels: in vitro and in vivo evaluation of wound healing and antibacterial properties. 负载银纳米粒子的羧甲基纤维素水凝胶的合成和表征:体外和体内伤口愈合和抗菌性能的评估。
IF 2 4区 医学 Q4 CELL BIOLOGY Pub Date : 2025-10-13 DOI: 10.1007/s10561-025-10197-w
Nariman Rezaei Kolarijani, Majid Salehi, Mehdi Mirzaii, Mohammad Kamalabadi Farahani, Sepehr Zamani, Mozhgan Fazli, Morteza Alizadeh

The current research was conducted to assess wound healing activity and antibacterial properties of carboxymethyl cellulose (CMC) hydrogels loaded with silver nanoparticles (AgNPs) against excisional wounds (15 × 15 mm2) infected with Pseudomonas aeruginosa and Staphylococcus aureus in a rat model.CMC/AgNPs hydrogels were synthesized using varying concentrations of AgNPs and subsequently lyophilized. A comprehensive range of in vitro tests were conducted, including nanoparticle characterization, scanning electron microscopy (SEM) morphology study, water uptake (WUE) study, blood uptake capacity study (BUC), weight loss study (WLA), pH, hemolysis percentage (HP), blood coagulation index (BCI), antibacterial activity (minimum inhibitory concentration [MIC] and minimum bactericidal concentration [MBC]), and cell viability through the MTT assay. In vivo wound healing studies were conducted using infected excisional wound models in rats. SEM confirmed a porous structure with a mean pore size ranging from 68 to 152 μm. The hydrogels exhibited dosage-dependent swelling and sustained physiological pH (7.4-7.6) for a period of time. The 125 μg/mL AgNPs formulation showed a BUC of 97.68% in 22 h. Hemocompatibility assay showed minimal hemolysis and acceptable coagulation indices for all concentrations of AgNPs. MIC and MBC against both strains of bacteria were found to be 250 μg/mL and 500 μg/mL, respectively. CMC/AgNPs hydrogel with the concentration of 250 μg/mL showed the optimal cell viability and the optimal in vivo wound healing result. The findings indicate that AgNPs-loaded CMC hydrogels possess favorable physicochemical, biocompatible, and antimicrobial properties, suggesting their potential as a wound dressing for managing infected wounds and supporting the wound healing process.

本研究旨在评估载银纳米颗粒(AgNPs)羧甲基纤维素(CMC)水凝胶对大鼠模型(15 × 15 mm2)铜绿假单胞菌和金黄色葡萄球菌感染的切除伤口的愈合活性和抗菌性能。用不同浓度的AgNPs合成CMC/AgNPs水凝胶,然后进行冻干。进行了一系列全面的体外测试,包括纳米颗粒表征、扫描电子显微镜(SEM)形态学研究、水摄取(WUE)研究、血液摄取能力(BUC)研究、体重减轻(WLA)研究、pH、溶血百分比(HP)、凝血指数(BCI)、抗菌活性(最小抑制浓度[MIC]和最小杀菌浓度[MBC]),以及通过MTT试验进行的细胞活力。采用大鼠感染切除伤口模型进行了体内伤口愈合研究。扫描电镜证实其孔隙结构平均孔径为68 ~ 152 μm。水凝胶表现出剂量依赖性肿胀和生理pH值(7.4-7.6)持续一段时间。125 μg/mL AgNPs制剂在22 h内BUC为97.68%。血液相容性试验显示,所有浓度的AgNPs溶血最小,凝血指标均可接受。对两种菌株的MIC和MBC分别为250 μg/mL和500 μg/mL。CMC/AgNPs水凝胶浓度为250 μg/mL时,细胞活力最佳,体内创面愈合效果最佳。研究结果表明,负载agnps的CMC水凝胶具有良好的物理化学、生物相容性和抗菌性能,这表明它们有潜力作为伤口敷料来管理感染伤口和支持伤口愈合过程。
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引用次数: 0
Effects of super-activated platelet lysate and bone mesenchymal stem cells on bone regeneration, and expression of bone metabolism-related factors in rats. 超活化血小板裂解液和骨间充质干细胞对大鼠骨再生及骨代谢相关因子表达的影响。
IF 2 4区 医学 Q4 CELL BIOLOGY Pub Date : 2025-10-04 DOI: 10.1007/s10561-025-10186-z
Huiying Lu, Tingting Li, Junqi Liu, Zitao Li, Yi Zhang

The present study aimed to evaluate the effectiveness of super-activated platelet lysate (sPL), as well as sPL in combination with bone marrow mesenchymal stem cells (BMSCs) in treating bone defects in rats. Ninety rats with induced radial defects were divided into three groups: sPL/BMSCs, sPL, and control. The healing process was evaluated using X-rays, Lane Sandhu scores, HE staining, RT-qPCR, immunohistochemistry, and ELISA. Both sPL and sPL/BMSCs facilitate bone repair. Compared to the other groups, the sPL/BMSCs group showed higher OCN, OSX, and RUNX2 gene expression until 10 weeks after treatment ALP levels in the sPL group increased until week 6 and remained elevated until week 10, with the sPL/BMSCs group showing significantly higher peak ALP levels. IGF-1, TGF-β, and VEGF levels in both treatment groups were higher than in the control group. Our study demonstrated that both sPL and sPL/BMSC transplants at the bone defect site can promote bone healing and modulate bone metabolism-related factors. However, the effect of sPL/BMSCs was superior to sPL alone.

本研究旨在评价超活化血小板裂解液(sPL)及其与骨髓间充质干细胞(BMSCs)联合治疗大鼠骨缺损的疗效。90只桡骨缺损大鼠分为sPL/BMSCs组、sPL组和对照组。采用x射线、Lane Sandhu评分、HE染色、RT-qPCR、免疫组织化学和ELISA评估愈合过程。sPL和sPL/BMSCs均促进骨修复。与其他组相比,治疗后10周,sPL/BMSCs组的OCN、OSX和RUNX2基因表达较高,sPL组的ALP水平升高至第6周,并持续升高至第10周,其中sPL/BMSCs组ALP水平峰值明显升高。两组患者IGF-1、TGF-β、VEGF水平均高于对照组。我们的研究表明,骨缺损部位的sPL和sPL/BMSC移植都可以促进骨愈合和调节骨代谢相关因素。然而,sPL/BMSCs的效果优于单独使用sPL。
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引用次数: 0
Processing of fresh frozen allograft and evaluation of its effect with platelet-rich fibrin membrane in the treatment of periodontal osseous defects. 新鲜冷冻同种异体移植物的制备及富血小板纤维蛋白膜治疗牙周骨缺损的效果评价。
IF 2 4区 医学 Q4 CELL BIOLOGY Pub Date : 2025-10-04 DOI: 10.1007/s10561-025-10195-y
Shaila V Kothiwale, Shaik Shahanaz, Dhanashree Patil, D Nivethitha

Bone grafts and its substitutes are commonly used in periodontics to enhance bone formation and periodontal regeneration. Allografts, derived from human donors, support new bone growth and are biocompatible, with reduced disease transmission risk due to rigorous screening. Combining bone grafts with growth factors can further improve treatment outcomes. Platelet Rich Fibrin (PRF) releases growth factors that enhance regeneration. This study evaluates the effectiveness of combining processed fresh frozen bone allograft (FFBA) with PRF mambrane for treating periodontal bone defects. The FFBA was prepared from bone chips collected from systemically healthy patients in an aseptic environment during orthopedic surgeries, which was then processed and tested for microbiological and cytotoxicity concerns using Human Osteosarcoma (MG-63) and Normal Mouse Fibroblast (L929) cell lines. PRF was prepared by centrifuging blood collected from patients at 2300 rpm for 12 min. A case study comprising of ten patients with chronic periodontitis was conducted, Clinical and radiographic measurements, including plaque index (PI), gingival index (GI), probing depth (PD), and bone defect fill percentage using radiographs were recorded at baseline and nine months. Descriptive statistics (Mean ± SD), a 5% significance level, and 90% confidence intervals were used. Paired t-tests showed significant improvements in GI (p < 0.001), PI (p < 0.001), PPD (p < 0.001), and Bone defect fill (p < 0.001). The study concluded that using processed fresh frozen bone allograft with PRF positively affects the treatment of periodontal osseous defects.

骨移植及其替代物常用于牙周病,以促进骨形成和牙周再生。来源于人类供体的同种异体移植物支持新骨生长,具有生物相容性,由于严格的筛选,降低了疾病传播风险。骨移植联合生长因子可进一步改善治疗效果。富血小板纤维蛋白(PRF)释放生长因子,促进再生。本研究评价了加工新鲜冷冻同种异体骨(FFBA)与PRF膜联合治疗牙周骨缺损的效果。FFBA是由骨科手术期间在无菌环境中收集的全身健康患者的骨片制备的,然后使用人骨肉瘤(MG-63)和正常小鼠成纤维细胞(L929)细胞系对其进行处理和微生物学和细胞毒性测试。将患者采集的血液以2300 rpm离心12 min制备PRF。我们对10例慢性牙周炎患者进行了病例研究,在基线和9个月时使用x线片记录临床和x线片测量,包括菌斑指数(PI)、牙龈指数(GI)、探探深度(PD)和骨缺损填充率。采用描述性统计(Mean±SD),显著性水平为5%,置信区间为90%。配对t检验显示GI显著改善(p
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Cell and Tissue Banking
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