Pub Date : 2021-01-01DOI: 10.15255/CABEQ.2020.1899
M. Matsumoto, K. Nakao, Y. Tahara
The effect of bio-imprinting and water activity on catalytic activities and the thermostability of lipases was investigated for transesterification using vinyl acetate and benzyl alcohol as substrates in ionic liquid, [Cnmim][PF6] (n=4,6,8), and benzene. The catalytic activities were enhanced by imprinting in benzene and [C4mim][PF6], and the relations between the transesterification activities and the water activity in both solvents were approximately bell shaped. The reactivity of the transesterification in benzene was higher than that in [C4mim][PF6]. The effects of water activity and imprinting on the kinetic parameters in [C4mim][PF6] were examined. Without controlling the water content, the values of Km,VA and Km,BA (Michaelis constants of vinyl acetate and benzyl alcohol, respectively) decreased, and the values of Vm (maximum rate) increased by imprinting. On the other hand, by controlling the water content in the organic media, the values of Vm, Km,VA, and Km,BA increased by imprinting. The activities of lipase in ionic liquid are more strongly affected by water activity and imprinting than those in benzene. We observed effects of water activity on thermostability but none from imprinting.
{"title":"Effects of Imprinting and Water Activity on Transesterification and Thermostability with Lipases in Ionic Liquid","authors":"M. Matsumoto, K. Nakao, Y. Tahara","doi":"10.15255/CABEQ.2020.1899","DOIUrl":"https://doi.org/10.15255/CABEQ.2020.1899","url":null,"abstract":"The effect of bio-imprinting and water activity on catalytic activities and the thermostability of lipases was investigated for transesterification using vinyl acetate and benzyl alcohol as substrates in ionic liquid, [Cnmim][PF6] (n=4,6,8), and benzene. The catalytic activities were enhanced by imprinting in benzene and [C4mim][PF6], and the relations between the transesterification activities and the water activity in both solvents were approximately bell shaped. The reactivity of the transesterification in benzene was higher than that in [C4mim][PF6]. The effects of water activity and imprinting on the kinetic parameters in [C4mim][PF6] were examined. Without controlling the water content, the values of Km,VA and Km,BA (Michaelis constants of vinyl acetate and benzyl alcohol, respectively) decreased, and the values of Vm (maximum rate) increased by imprinting. On the other hand, by controlling the water content in the organic media, the values of Vm, Km,VA, and Km,BA increased by imprinting. The activities of lipase in ionic liquid are more strongly affected by water activity and imprinting than those in benzene. We observed effects of water activity on thermostability but none from imprinting.","PeriodicalId":9765,"journal":{"name":"Chemical and Biochemical Engineering Quarterly","volume":"35 1","pages":"57-63"},"PeriodicalIF":1.5,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67064578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.15255/CABEQ.2020.1909
M. Dükkancı
Efficient decolorization of Rhodamine 6G dye (Rh 6G) using magnetic TiO2@ Fe3O4/FeZSM-5 photocatalyst was carried out in a batch reactor equipped with two visible lights (high-pressure Na lamps). The photocatalyst was synthesized and characterized by X-ray diffraction, scanning electron microscopy, energy dispersive X-ray spectroscopy, diffuse reflectance spectroscopy and vibrating sample magnetometry. The effects of initial Rh 6G concentration, catalyst loading, and pH were investigated on photocatalytic oxidation of Rh 6G. Maximum decolorization of 98.2 % was found at optimum conditions of 20 ppm Rh 6G, 1 g L–1 of catalyst, and pH of 13.04. Central composite design was used for the optimization of photocatalytic degradation of Rh 6G. Predicted decolorization efficiencies were found to be in good agreement with experimental values with high regression coefficient of 98.8 %. In addition to color removal, the toxicity of the Rh 6G aqueous solution was significantly reduced after photocatalytic oxidation. Small reduction of activity (from 34.6 % to 30.5 %) showed the stability of the catalyst after three consecutive runs. Due to the magnetic property of the catalyst, it could be removed from the solution with the help of external magnetic field. This prevents the loss of catalyst and reduces the extra separation cost, which is desired in industrial or large-scale applications.
在配备两盏可见光(高压钠灯)的间歇式反应器中，采用磁性TiO2@ Fe3O4/FeZSM-5光催化剂对罗丹明6G染料(Rh 6G)进行了高效脱色。合成了光催化剂，并用x射线衍射、扫描电镜、x射线能谱、漫反射光谱和振动样品磁强计对其进行了表征。考察了初始Rh 6G浓度、催化剂负载和pH对Rh 6G光催化氧化的影响。在Rh为20 ppm、催化剂为1 g L-1、pH为13.04的条件下，脱色率达到98.2%。采用中心复合设计优化光催化降解Rh 6G的工艺。预测脱色效率与实验值吻合良好，回归系数高达98.8%。除除颜色外，光催化氧化后的Rh 6G水溶液的毒性也显著降低。连续运行三次后，活性略有下降(从34.6%降至30.5%)，表明催化剂的稳定性。由于催化剂的磁性，它可以在外加磁场的帮助下从溶液中去除。这防止了催化剂的损失，降低了额外的分离成本，这是工业或大规模应用所需要的。
{"title":"Photocatalytic Oxidation of Rhodamine 6G Dye Using Magnetic TiO2@Fe3O4/FeZSM-5","authors":"M. Dükkancı","doi":"10.15255/CABEQ.2020.1909","DOIUrl":"https://doi.org/10.15255/CABEQ.2020.1909","url":null,"abstract":"Efficient decolorization of Rhodamine 6G dye (Rh 6G) using magnetic TiO2@ Fe3O4/FeZSM-5 photocatalyst was carried out in a batch reactor equipped with two visible lights (high-pressure Na lamps). The photocatalyst was synthesized and characterized by X-ray diffraction, scanning electron microscopy, energy dispersive X-ray spectroscopy, diffuse reflectance spectroscopy and vibrating sample magnetometry. The effects of initial Rh 6G concentration, catalyst loading, and pH were investigated on photocatalytic oxidation of Rh 6G. Maximum decolorization of 98.2 % was found at optimum conditions of 20 ppm Rh 6G, 1 g L–1 of catalyst, and pH of 13.04. Central composite design was used for the optimization of photocatalytic degradation of Rh 6G. Predicted decolorization efficiencies were found to be in good agreement with experimental values with high regression coefficient of 98.8 %. In addition to color removal, the toxicity of the Rh 6G aqueous solution was significantly reduced after photocatalytic oxidation. Small reduction of activity (from 34.6 % to 30.5 %) showed the stability of the catalyst after three consecutive runs. Due to the magnetic property of the catalyst, it could be removed from the solution with the help of external magnetic field. This prevents the loss of catalyst and reduces the extra separation cost, which is desired in industrial or large-scale applications.","PeriodicalId":9765,"journal":{"name":"Chemical and Biochemical Engineering Quarterly","volume":"92 1","pages":"17-29"},"PeriodicalIF":1.5,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67064134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.15255/CABEQ.2020.1834
Emi Govorčin Bajsić, T. Grgurić, B. Mijović, I. Vrgoč Zimić, Tamara Dolenec, S. Kuzmić, Nikolina Mrkonjić, M. T. Trcin, I. Slivac, E. Zdraveva
Natural polymer-based scaffolds are generally considered as favourable matrices for the adhesion and growth of cells in tissue repair. One of the most popular materials in this respect is silk fibroin, known for its wide usage in biomedical applications. This work focuses on the development of electrospun scaffolds based on poly(ε-caprolactone) (PCL) and silk fibroin (SF) evaluated regarding the SF effect on their morphology, surface wetting ability, thermal properties, and HaCaT model cell line biocompatibility. The study revealed that the lowest PCL/SF concentration resulted in highest bead-like morphology formation, relatively thick fibers with the presence of random beads in the case of PCL, while uniform and thinner fibers in the case of increasing PCL/SF content scaffolds. The addition of SF reduced the degree of crystallinity in the PCL due to the less organized crystal structure, and decreased its thermal stability. Both SEM and MTT analyses showed cell presence on all scaffolds three days after cell seeding. Although SF improved PCL hydrophilicity, as shown quantitatively by the MTT assay for improved cytocompatibility properties, more structured electrospun PCL/SF scaffold strategies are required.
{"title":"Preparation and Characterization of Electrospun PCL/Silk Fibroin Scaffolds","authors":"Emi Govorčin Bajsić, T. Grgurić, B. Mijović, I. Vrgoč Zimić, Tamara Dolenec, S. Kuzmić, Nikolina Mrkonjić, M. T. Trcin, I. Slivac, E. Zdraveva","doi":"10.15255/CABEQ.2020.1834","DOIUrl":"https://doi.org/10.15255/CABEQ.2020.1834","url":null,"abstract":"Natural polymer-based scaffolds are generally considered as favourable matrices for the adhesion and growth of cells in tissue repair. One of the most popular materials in this respect is silk fibroin, known for its wide usage in biomedical applications. This work focuses on the development of electrospun scaffolds based on poly(ε-caprolactone) (PCL) and silk fibroin (SF) evaluated regarding the SF effect on their morphology, surface wetting ability, thermal properties, and HaCaT model cell line biocompatibility. The study revealed that the lowest PCL/SF concentration resulted in highest bead-like morphology formation, relatively thick fibers with the presence of random beads in the case of PCL, while uniform and thinner fibers in the case of increasing PCL/SF content scaffolds. The addition of SF reduced the degree of crystallinity in the PCL due to the less organized crystal structure, and decreased its thermal stability. Both SEM and MTT analyses showed cell presence on all scaffolds three days after cell seeding. Although SF improved PCL hydrophilicity, as shown quantitatively by the MTT assay for improved cytocompatibility properties, more structured electrospun PCL/SF scaffold strategies are required.","PeriodicalId":9765,"journal":{"name":"Chemical and Biochemical Engineering Quarterly","volume":"35 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67064297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-11-04DOI: 10.15255/cabeq.2020.1819
M. Koller, A. Mukherjee
When it comes to “bioplastics”, we currently notice an immense complexity of this topic, and, most of all, a plethora of contradictory legislations, which confuses or even misleads insufficiently informed consumers. The present article therefore showcases microbial polyhydroxyalkanoate (PHA) biopolyesters as the prime class of “bioplastics” sensu stricto. In particular, biodegradability of PHA as its central benefit in elevating the current plastic waste scenario is elaborated on the biochemical basis: this covers aspects of the enzymatic machinery involved both in intra- and extracellular PHA degradation, and environmental factors impacting biodegradability. Importantly, PHA degradability is contextualized with potential fields of application of these materials. It is further shown how the particularities of PHA in terms of feedstocks, mode of synthesis, degradability, and compostability differ from other polymeric materials sold as “bioplastics”, highlighting the unique selling points of PHA as “green” plastic products in the circular economy. Moreover, current standards, norms, and certificates applicable to PHA are presented as basis for a straight-forward, scientifically grounded classification of “bioplastics”.
{"title":"Polyhydroxyalkanoates – Linking Properties, Applications and End-of-life Options","authors":"M. Koller, A. Mukherjee","doi":"10.15255/cabeq.2020.1819","DOIUrl":"https://doi.org/10.15255/cabeq.2020.1819","url":null,"abstract":"When it comes to “bioplastics”, we currently notice an immense complexity of this topic, and, most of all, a plethora of contradictory legislations, which confuses or even misleads insufficiently informed consumers. The present article therefore showcases microbial polyhydroxyalkanoate (PHA) biopolyesters as the prime class of “bioplastics” sensu stricto. In particular, biodegradability of PHA as its central benefit in elevating the current plastic waste scenario is elaborated on the biochemical basis: this covers aspects of the enzymatic machinery involved both in intra- and extracellular PHA degradation, and environmental factors impacting biodegradability. Importantly, PHA degradability is contextualized with potential fields of application of these materials. It is further shown how the particularities of PHA in terms of feedstocks, mode of synthesis, degradability, and compostability differ from other polymeric materials sold as “bioplastics”, highlighting the unique selling points of PHA as “green” plastic products in the circular economy. Moreover, current standards, norms, and certificates applicable to PHA are presented as basis for a straight-forward, scientifically grounded classification of “bioplastics”.","PeriodicalId":9765,"journal":{"name":"Chemical and Biochemical Engineering Quarterly","volume":"34 1","pages":"115-129"},"PeriodicalIF":1.5,"publicationDate":"2020-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47243964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-07-31DOI: 10.15255/cabeq.2020.1808
W. Estela-Escalante, J. J. Pérez-Escalante, E. Fuentes-Navarro, R. M. Pinillos-Miñano
W. D. Estela-Escalante,a,* J. J. Pérez-Escalante,b E. L. Fuentes-Navarro,c and R. M. Pinillos-Miñanoa aUniversidad Nacional Mayor de San Marcos, Facultad de Química e Ingeniería Química, Av. Germán Amézaga 375, Lima 1, Perú bUniversidad Nacional Mayor de San Marcos, Facultad de Farmacia y Bioquímica, Jr. Puno 1002, Lima 1, Perú cUniversidad Nacional Agraria La Molina, Escuela de Posgrado, Av. La Molina s/n, La Molina, Lima 12, Perú
{"title":"The Potential of Using Grapefruit Peel as a Natural Support for Yeast Immobilization During Beer Fermentation","authors":"W. Estela-Escalante, J. J. Pérez-Escalante, E. Fuentes-Navarro, R. M. Pinillos-Miñano","doi":"10.15255/cabeq.2020.1808","DOIUrl":"https://doi.org/10.15255/cabeq.2020.1808","url":null,"abstract":"W. D. Estela-Escalante,a,* J. J. Pérez-Escalante,b E. L. Fuentes-Navarro,c and R. M. Pinillos-Miñanoa aUniversidad Nacional Mayor de San Marcos, Facultad de Química e Ingeniería Química, Av. Germán Amézaga 375, Lima 1, Perú bUniversidad Nacional Mayor de San Marcos, Facultad de Farmacia y Bioquímica, Jr. Puno 1002, Lima 1, Perú cUniversidad Nacional Agraria La Molina, Escuela de Posgrado, Av. La Molina s/n, La Molina, Lima 12, Perú","PeriodicalId":9765,"journal":{"name":"Chemical and Biochemical Engineering Quarterly","volume":"34 1","pages":"105-114"},"PeriodicalIF":1.5,"publicationDate":"2020-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41842193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-07-31DOI: 10.15255/cabeq.2020.1783
A. Ressler, A. Gudelj, Karla Zadro, M. Antunović, M. Cvetnić, M. Ivanković, H. Ivanković
Nanocomposite structure of the bone can be mimicked by chitosan/hydroxyapatite (CS/HAp) composite scaffold. Biological hydroxyapatite (HAp) contains various ions, which have a crucial role in bone growth. The aim of the present work was to synthesize biomimetic hydroxyapatite and prepare composite scaffolds based on chitosan, where HAp was synthesised from hen eggshells, seashells and cuttlefish bone. The powders were composed of nano-structured calcium deficient HAp and amorphous calcium phosphate (ACP). In the as-prepared powders, Sr2+, Mg2+ and Na+ ions were detected as a result of using biogenic precursor of Ca2+ ions. Highly porous CS/HAp structures have been prepared by freeze-gelation technique. The CS/HAp scaffolds have shown highly porous structure with very well interconnected pores and homogeneously dispersed HAp particles. The MTT assay of CS/HAp scaffolds has shown no toxicity, and the live/dead assay has confirmed good viability and proliferation of seeded cells.
{"title":"From Bio-waste to Bone Substitute","authors":"A. Ressler, A. Gudelj, Karla Zadro, M. Antunović, M. Cvetnić, M. Ivanković, H. Ivanković","doi":"10.15255/cabeq.2020.1783","DOIUrl":"https://doi.org/10.15255/cabeq.2020.1783","url":null,"abstract":"Nanocomposite structure of the bone can be mimicked by chitosan/hydroxyapatite (CS/HAp) composite scaffold. Biological hydroxyapatite (HAp) contains various ions, which have a crucial role in bone growth. The aim of the present work was to synthesize biomimetic hydroxyapatite and prepare composite scaffolds based on chitosan, where HAp was synthesised from hen eggshells, seashells and cuttlefish bone. The powders were composed of nano-structured calcium deficient HAp and amorphous calcium phosphate (ACP). In the as-prepared powders, Sr2+, Mg2+ and Na+ ions were detected as a result of using biogenic precursor of Ca2+ ions. Highly porous CS/HAp structures have been prepared by freeze-gelation technique. The CS/HAp scaffolds have shown highly porous structure with very well interconnected pores and homogeneously dispersed HAp particles. The MTT assay of CS/HAp scaffolds has shown no toxicity, and the live/dead assay has confirmed good viability and proliferation of seeded cells.","PeriodicalId":9765,"journal":{"name":"Chemical and Biochemical Engineering Quarterly","volume":"34 1","pages":"59-71"},"PeriodicalIF":1.5,"publicationDate":"2020-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.15255/cabeq.2020.1783","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44940852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-04-19DOI: 10.15255/cabeq.2019.1740
T. Chysirichote
Valorization of banana peel (BP) through solid state fermentation (SSF) was aimed in this research. The appropriate conditions of citric acid (CA) production by SSF of Aspergillus niger were investigated. Firstly, the optimum initial pH of the BP and the aeration rate were studied by conducting SSF in a 250-mL flask and 2-L glass column, respectively. It was found that the initial pH of the BP and aeration rates affected the CA production. The results showed that the initial pH of 5.0 and 1.0 vvm aeration were appropriate for the CA production of A. niger using BP as a substrate. The problem of rising temperature during SSF was found when applying the optimum condition to the SSF in the 20-L packed bed bioreactor (PBB), which caused a decrease in the CA production compared to that of the glass column. The cooling air jacket constructed to the PBB to remove the heat during the SSF helped increase the CA production from that in the PBB. The maximum CA production in the 20-L air-jacketed PBB was 124.0±19.2 mg gDS.
{"title":"Valorization of Banana Peel for Citric Acid Production under Solid State Fermentation with Aspergillus niger","authors":"T. Chysirichote","doi":"10.15255/cabeq.2019.1740","DOIUrl":"https://doi.org/10.15255/cabeq.2019.1740","url":null,"abstract":"Valorization of banana peel (BP) through solid state fermentation (SSF) was aimed in this research. The appropriate conditions of citric acid (CA) production by SSF of Aspergillus niger were investigated. Firstly, the optimum initial pH of the BP and the aeration rate were studied by conducting SSF in a 250-mL flask and 2-L glass column, respectively. It was found that the initial pH of the BP and aeration rates affected the CA production. The results showed that the initial pH of 5.0 and 1.0 vvm aeration were appropriate for the CA production of A. niger using BP as a substrate. The problem of rising temperature during SSF was found when applying the optimum condition to the SSF in the 20-L packed bed bioreactor (PBB), which caused a decrease in the CA production compared to that of the glass column. The cooling air jacket constructed to the PBB to remove the heat during the SSF helped increase the CA production from that in the PBB. The maximum CA production in the 20-L air-jacketed PBB was 124.0±19.2 mg gDS.","PeriodicalId":9765,"journal":{"name":"Chemical and Biochemical Engineering Quarterly","volume":"34 1","pages":"49-57"},"PeriodicalIF":1.5,"publicationDate":"2020-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47802794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.15255/cabeq.2019.1699
R. Faria, M. Houmard, V. A. M. D. Rosário, V. Lins, L. Heneine, T. Matencio
R. A. D. de Faria,a,* M. Houmard,b V. A. M. do Rosário,c V. de Freitas Cunha Lins,a L. G. D. Heneine,c and T. Matenciod aDepartment of Chemical Engineering, Universidade Federal de Minas Gerais, Belo Horizonte, MG 31270-90, Brazil bDepartment of Materials and Construction Engineering, Universidade Federal de Minas Gerais, Belo Horizonte, MG 31270-90, Brazil cDepartment of Applied Immunology, Fundação Ezequiel Dias, Belo Horizonte, MG 30510-010, Brazil dDepartment of Chemistry, Universidade Federal de Minas Gerais, Belo Horizonte, MG 31270-90, Brazil
r·a·d·德·法里亚,* m . Houmard b v . a . m .,罗萨里奥c v . de Freitas达林家,l·g·d·Heneine c和t . Matenciod aDepartment化学工程、大学联邦德·米纳斯吉拉斯,贝洛奥里藏特,31270 - 90毫克,巴西bDepartment材料和建筑工程,大学联邦德·米纳斯吉拉斯,贝洛奥里藏特,31270 - 90毫克,巴西cDepartment应用免疫学,Fundacao Ezequiel迪亚斯,贝洛奥里藏特,30510 - 010毫克,巴西dDepartment化学,米纳斯吉拉斯州联邦大学，贝洛奥里藏特，MG 31270-90，巴西
{"title":"TiO2 Sol-gel Coating as a Transducer Substrate for Impedimetric Immunosensors","authors":"R. Faria, M. Houmard, V. A. M. D. Rosário, V. Lins, L. Heneine, T. Matencio","doi":"10.15255/cabeq.2019.1699","DOIUrl":"https://doi.org/10.15255/cabeq.2019.1699","url":null,"abstract":"R. A. D. de Faria,a,* M. Houmard,b V. A. M. do Rosário,c V. de Freitas Cunha Lins,a L. G. D. Heneine,c and T. Matenciod aDepartment of Chemical Engineering, Universidade Federal de Minas Gerais, Belo Horizonte, MG 31270-90, Brazil bDepartment of Materials and Construction Engineering, Universidade Federal de Minas Gerais, Belo Horizonte, MG 31270-90, Brazil cDepartment of Applied Immunology, Fundação Ezequiel Dias, Belo Horizonte, MG 30510-010, Brazil dDepartment of Chemistry, Universidade Federal de Minas Gerais, Belo Horizonte, MG 31270-90, Brazil","PeriodicalId":9765,"journal":{"name":"Chemical and Biochemical Engineering Quarterly","volume":"67 3 1","pages":"437-447"},"PeriodicalIF":1.5,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67063753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.15255/cabeq.2020.1823
W. Zhang, Zhen Zhang, Sufeng Wang
How to simplify the nitrogen removal process, reduce the cost and improve the efficiency has become an urgent problem to be solved. In this research, the isolated HNAD (heterotrophic nitrification and aerobic denitrification) bacteria were used to remove the nitrogen in wastewater. Modified absorbent stone was used as high-efficiency and low-cost immobilized material. The modification effect was determined by the changes in mechanical strength, Zeta potential, pore structure, micrographs and biomass. The practicability of the modified carrier was further proved by experiments of environmental effect and reuse. The modified carrier had excellent performance. By comparing the degradation effects of immobilized microorganism and free microorganism, it was proved that the immobilized microorganisms have broad application prospects and strong adaptability to environmental factors. Under the optimum conditions (temperature of 30 oC, pH of 7, dissolved oxygen of 3.5 mg L–1), the removal efficiency of ammonia nitrogen reached 100 % in 40 hours, the removal efficiency of total nitrogen reached 60.11 % in 50 hours, and the removal rate of total nitrogen was 2.404 mg-NL–1 h–1 by immobilized microorganisms with the treatment of simulated nitrogen-containing wastewater. This research provides new material for the immobilization of HN-AD bacteria and a new way for nitrogen removal.
{"title":"Nitrogen Removal by HN-AD Bacteria Immobilized on Modified Absorbent Stone","authors":"W. Zhang, Zhen Zhang, Sufeng Wang","doi":"10.15255/cabeq.2020.1823","DOIUrl":"https://doi.org/10.15255/cabeq.2020.1823","url":null,"abstract":"How to simplify the nitrogen removal process, reduce the cost and improve the efficiency has become an urgent problem to be solved. In this research, the isolated HNAD (heterotrophic nitrification and aerobic denitrification) bacteria were used to remove the nitrogen in wastewater. Modified absorbent stone was used as high-efficiency and low-cost immobilized material. The modification effect was determined by the changes in mechanical strength, Zeta potential, pore structure, micrographs and biomass. The practicability of the modified carrier was further proved by experiments of environmental effect and reuse. The modified carrier had excellent performance. By comparing the degradation effects of immobilized microorganism and free microorganism, it was proved that the immobilized microorganisms have broad application prospects and strong adaptability to environmental factors. Under the optimum conditions (temperature of 30 oC, pH of 7, dissolved oxygen of 3.5 mg L–1), the removal efficiency of ammonia nitrogen reached 100 % in 40 hours, the removal efficiency of total nitrogen reached 60.11 % in 50 hours, and the removal rate of total nitrogen was 2.404 mg-NL–1 h–1 by immobilized microorganisms with the treatment of simulated nitrogen-containing wastewater. This research provides new material for the immobilization of HN-AD bacteria and a new way for nitrogen removal.","PeriodicalId":9765,"journal":{"name":"Chemical and Biochemical Engineering Quarterly","volume":"34 1","pages":"193-207"},"PeriodicalIF":1.5,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67063965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.15255/cabeq.2020.1843
B. Darabi, T. Tabatabaei, F. Amiri, S. Jorfi
B. Darabi,a T. Tabatabaei,b,* F. Amiri,c and S. Jorfid,e,* aDepartment of Environmental Engineering, Bushehr Branch, Islamic Azad University, Bushehr, Iran bDepartment of Environmental Engineering, Bushehr Branch, Islamic Azad University, Bushehr, Iran cDepartment of Environmental Engineering, Bushehr Branch, Islamic Azad University, Bushehr, Iran dEnvironmental Technologies Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran eDepartment of Environmental Health Engineering, School of Health, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
b . Darabi,a . T. Tabatabaei,b，* F. Amiri,c和S. Jorfid,e，* a伊朗布什尔伊斯兰阿扎德大学布什尔分校环境工程系b伊朗布什尔伊斯兰阿扎德大学布什尔分校环境工程系c伊朗布什尔伊斯兰阿扎德大学布什尔分校环境工程系d阿瓦士Jundishapur医科大学环境技术研究中心伊朗阿瓦士君迪沙普尔医科大学卫生学院环境卫生工程系，伊朗阿瓦士
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