Pub Date : 2019-04-10DOI: 10.15255/CABEQ.2018.1306
H. Hosseinzadeh, M. M. Tabatabai Asl
In the present work, the structural characterization and chromium adsorption behavior of a novel poly(pyrrole)-based magnetic hydrogel nanocomposite (MHN) is reported. This product was prepared using free-radical copolymerization of pyrrole (Py), acrylic acid (AA) and acrylamide (AM) monomers, and subsequent in situ synthesis of magnetic Fe3O4 nanoparticles. The structure of MHNs was then characterized by FTIR, SEM, TEM, XRD, UV-Vis and VSM techniques, and a mechanism for the preparation of MHNs is also proposed. The maximum Cr(VI) adsorption capacity (208 mg g–1) was achieved under the optimum conditions that were found to be: AA = 0.25 mol L–1, AM = 1.2 mol L–1, agitation time = 90 min, solution pH = 1.0, ion concentration = 100 mg L–1, adsorbent dose = 50 mg, and temperature = 65 °C. Further, the calculated values of the thermodynamic parameters (ΔH° = 31.33kJ mol–1, ΔS° = 105.67 J K–1 mol–1, ΔG° = –61.33 kJ mol–1) revealed that ion adsorption is a spontaneous and endothermic process. In general, the results indicated that the synthesized MHNs with specific properties can be used in wastewater treatment applications.
{"title":"Enhanced Removal of Cr(VI) from Aqueous Solutions Using Poly(Pyrrole)-g-Poly(Acrylic Acid-co- Acrylamide)/Fe3O4 Magnetic Hydrogel Nanocomposite Adsorbent","authors":"H. Hosseinzadeh, M. M. Tabatabai Asl","doi":"10.15255/CABEQ.2018.1306","DOIUrl":"https://doi.org/10.15255/CABEQ.2018.1306","url":null,"abstract":"In the present work, the structural characterization and chromium adsorption behavior\u0000of a novel poly(pyrrole)-based magnetic hydrogel nanocomposite (MHN) is reported. This product was prepared using free-radical copolymerization of pyrrole (Py), acrylic acid (AA) and acrylamide (AM) monomers, and subsequent in situ synthesis of magnetic Fe3O4 nanoparticles. The structure of MHNs was then characterized by FTIR, SEM, TEM, XRD, UV-Vis and VSM techniques, and a mechanism for the preparation of MHNs is also proposed. The maximum Cr(VI) adsorption capacity (208 mg g–1) was achieved under the optimum conditions that were found to be: AA = 0.25 mol L–1, AM = 1.2 mol L–1, agitation time = 90 min, solution pH = 1.0, ion concentration = 100 mg L–1, adsorbent dose = 50 mg, and temperature = 65 °C. Further, the calculated values of the thermodynamic parameters (ΔH° = 31.33kJ mol–1, ΔS° = 105.67 J K–1 mol–1, ΔG° = –61.33 kJ mol–1) revealed that ion adsorption is a spontaneous and endothermic process. In general, the results indicated that the synthesized MHNs with specific properties can be used in wastewater treatment applications.","PeriodicalId":9765,"journal":{"name":"Chemical and Biochemical Engineering Quarterly","volume":"365 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2019-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80338423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-04-10DOI: 10.15255/CABEQ.2017.1263
Kianoush Kosravi-Darani, F. Yazdian, Fatemeh Babapour, A. Amirsadeghi
Reducing the total cost of poly(3-hydroxybutyrate) (PHB) production as an attractive substitute for conventional petrochemical plastics still remains an unsolved problem. The aim of this research was the screening of PHB-producing microorganisms and selection of the best suitable medium for microbial growth and PHB production from methane. A new isolated methanotroph for PHB production from natural gas was studied in different media. After selection of the suitable medium, the effect of five process variables (content of nitrogen source, disodium hydrogen phosphate, methane to air ratio, seed age, and pH) on PHB production was investigated in a bubble column bioreactor. Also, hydrodynamic and mass transfer factors (flow regime, mixing time, gas hold up, and kLa) were considered. At optimum operating conditions and engineering parameters in a bubble column, PHB content in the dried biomass reached 25 % w/w. The results showed that pH is the most important variable in the selected conditions.
{"title":"Poly(3-hydroxybutyrate) Production from Natural Gas by a Methanotroph Native Bacterium in a Bubble Column Bioreactor","authors":"Kianoush Kosravi-Darani, F. Yazdian, Fatemeh Babapour, A. Amirsadeghi","doi":"10.15255/CABEQ.2017.1263","DOIUrl":"https://doi.org/10.15255/CABEQ.2017.1263","url":null,"abstract":"Reducing the total cost of poly(3-hydroxybutyrate) (PHB) production as an attractive\u0000\u0000substitute for conventional petrochemical plastics still remains an unsolved problem.\u0000\u0000The aim of this research was the screening of PHB-producing microorganisms and selection of the best suitable medium for microbial growth and PHB production from methane. A new isolated methanotroph for PHB production from natural gas was studied in different media. After selection of the suitable medium, the effect of five process variables (content of nitrogen source, disodium hydrogen phosphate, methane to air ratio, seed age, and pH) on PHB production was investigated in a bubble column bioreactor. Also, hydrodynamic and mass transfer factors (flow regime, mixing time, gas hold up, and kLa) were considered. At optimum operating conditions and engineering parameters in a bubble column, PHB content in the dried biomass reached 25 % w/w. The results showed that pH is the most important variable in the selected conditions.","PeriodicalId":9765,"journal":{"name":"Chemical and Biochemical Engineering Quarterly","volume":"6 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2019-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74332341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-04-10DOI: 10.15255/CABEQ.2018.1469
M. Berovič, J. Nemanič
Redox potential measurement was efficiently used for monitoring the two-year maturation process of cultivar Blau Fränkisch wine in 225-L oak barrels. Redox potential of maturing wine was measured at six levels from the top to the bottom of the barrel. During the process of wine maturation, the formation and heterogeneity of redox layers, as well as the formation of various oxidoreductive zones were indicated. The end of barrel maturation process was represented with homogeneity of all redox zones where no differences in all measured levels were indicated.
{"title":"The Role of Redox Potential Measurement in Oak Barrel Wine Maturation","authors":"M. Berovič, J. Nemanič","doi":"10.15255/CABEQ.2018.1469","DOIUrl":"https://doi.org/10.15255/CABEQ.2018.1469","url":null,"abstract":"Redox potential measurement was efficiently used for monitoring the two-year maturation process of cultivar Blau Fränkisch wine in 225-L oak barrels. Redox potential of maturing wine was measured at six levels from the top to the bottom of the barrel. During the process of wine maturation, the formation and heterogeneity of redox layers, as well as the formation of various oxidoreductive zones were indicated. The end of barrel maturation process was represented with homogeneity of all redox zones where no differences in all measured levels were indicated.","PeriodicalId":9765,"journal":{"name":"Chemical and Biochemical Engineering Quarterly","volume":"58 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2019-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86435240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-03-01DOI: 10.15255/CABEQ.2018.1525
S. Jokić, N. Pavlović, A. Jozinović, Đ. Ačkar, J. Babić, D. Šubarić
This study is focused on the application of high-voltage electric discharge (HVED) to recover some bioactive compounds from the cocoa bean shell. Different extraction times (30, 60, 90 min), frequencies (40, 70, 100 Hz) and solvent-solid ratios (10, 30 and 50 mL g–1) were used to obtain cocoa bean shell extracts. Desired bioactive compounds, methylxanthines and phenolic compounds were measured in obtained extracts by high-pressure liquid chromatography with diode array detector. The obtained extracts showed that theobromine was the most abundant, ranging from 2530.13 to 6031.51 mg kg–1, while caffeine content was in the range from 316.08 to 849.88 mg kg–1. In addition, significant amounts of phenolic compounds were found, namely catechin (115.91 to 284.33 mg kg–1), epicatechin (20.20 to 358.90 mg kg–1), and gallic acid (80.28 to 219.17 mg kg–1). Results showed that different parameters of HVED extraction have statistically significant influence on cocoa bean shell composition, suggesting how this byproduct can be used in the production of valuable extracts.
{"title":"High-Voltage Electric Discharge Extraction of Bioactive Compounds from the Cocoa Bean Shell","authors":"S. Jokić, N. Pavlović, A. Jozinović, Đ. Ačkar, J. Babić, D. Šubarić","doi":"10.15255/CABEQ.2018.1525","DOIUrl":"https://doi.org/10.15255/CABEQ.2018.1525","url":null,"abstract":"This study is focused on the application of high-voltage electric discharge (HVED) to recover some bioactive compounds from the cocoa bean shell. Different extraction times (30, 60, 90 min), frequencies (40, 70, 100 Hz) and solvent-solid ratios (10, 30 and 50 mL g–1) were used to obtain cocoa bean shell extracts. Desired bioactive compounds, methylxanthines and phenolic compounds were measured in obtained extracts by high-pressure liquid chromatography with diode array detector. The obtained extracts showed that theobromine was the most abundant, ranging from 2530.13 to 6031.51 mg kg–1, while caffeine content was in the range from 316.08 to 849.88 mg kg–1. In addition, significant amounts of phenolic compounds were found, namely catechin (115.91 to 284.33 mg kg–1), epicatechin (20.20 to 358.90 mg kg–1), and gallic acid (80.28 to 219.17 mg kg–1). Results showed that different parameters of HVED extraction have statistically significant influence on cocoa bean shell composition, suggesting how this byproduct can be used in the production of valuable extracts.","PeriodicalId":9765,"journal":{"name":"Chemical and Biochemical Engineering Quarterly","volume":"12 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2019-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87235830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-17DOI: 10.15255/CABEQ.2018.1393
M. Sudar, I. Dejanović, Michael Vasić-Rački Đurđa Müller, Z. Blažević
To speed up evaluation, development and upscaling of new processes, the use of engineering methodology can have a great impact. Here we show the application of an engineering approach to find the reaction conditions allowing the best process metrics. An experimentally validated mathematical model for the MenD-catalyzed synthesis of a commercially unavailable product 6-cyano-4- oxohexanoic acid, with a potential industrial use as a building block, was used for process optimization. Using the optimized conditions, 62.4 g dm3 of product, volume productivity of 87.1 g dm3 d1, product yield of 96 % and biocatalyst productivity of 25.8 kgP kgMenD1 can be achieved. Based on the optimized production procedure, economic analysis was performed to determine minimal product price required for project to be profitable in 8 years economic lifetime. In addition, Monte Carlo analysis (MCA) was used to assess the influence of uncertainties in estimation of input variables on overall economic performance.
为了加快新工艺的评估、开发和升级，工程方法论的使用可以产生巨大影响。在这里，我们展示了工程方法的应用，以找到允许最佳工艺指标的反应条件。一个实验验证的数学模型用于MenD催化合成商业上不可用的产品6-氰基-4-氧代己酸，该产品具有潜在的工业用途作为构建块，用于工艺优化。使用优化的条件，62.4 g dm产品的3，体积生产率为87.1克dm3天1，产品收率为96%，生物催化剂生产率为25.8 kgP kgMenD1。在优化生产程序的基础上，进行了经济分析，以确定项目在8年经济寿命内盈利所需的最低产品价格。此外，还使用蒙特卡罗分析（MCA）来评估投入变量估计中的不确定性对整体经济绩效的影响。
{"title":"Application of Chemical Engineering Methodology in Process Development: A Case Study of MenD-catalyzed Synthesis of 6-Cyano-4-oxohexanoic Acid","authors":"M. Sudar, I. Dejanović, Michael Vasić-Rački Đurđa Müller, Z. Blažević","doi":"10.15255/CABEQ.2018.1393","DOIUrl":"https://doi.org/10.15255/CABEQ.2018.1393","url":null,"abstract":"To speed up evaluation, development and upscaling of new processes, the use of engineering methodology can have a great impact. Here we show the application of an engineering approach to find the reaction conditions allowing the best process metrics. An experimentally validated mathematical model for the MenD-catalyzed synthesis of a commercially unavailable product 6-cyano-4- oxohexanoic acid, with a potential industrial use as a building block, was used for process optimization. Using the optimized conditions, 62.4 g dm3 of product, volume productivity of 87.1 g dm3 d1, product yield of 96 % and biocatalyst productivity of 25.8 kgP kgMenD1 can be achieved. Based on the optimized production procedure, economic analysis was performed to determine minimal product price required for project to be profitable in 8 years economic lifetime. In addition, Monte Carlo analysis (MCA) was used to assess the influence of uncertainties in estimation of input variables on overall economic performance.","PeriodicalId":9765,"journal":{"name":"Chemical and Biochemical Engineering Quarterly","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2019-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45795986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-16DOI: 10.15255/CABEQ.2017.1241
G. Maria, M. Mihalachi, C. Gijiu
Autonomous oscillations of glycolytic intermediate concentrations reflect the dynamics of the control and regulation of this major catabolic pathway, and this phenomenon has been reported in a broad range of bacteria. Understanding glycolytic oscillations might therefore prove crucial for the general understanding of the regulation of cell metabolism with immediate practical applications, allowing in silico design of modified cells with desirable ‘motifs’ of practical applications in the biosynthesis industry, environmental engineering, and medicine. By using a kinetic model from literature, this paper is aiming at in silico (model-based) identification of some conditions leading to the occurrence of stable glycolytic oscillations in the E. coli cells.
{"title":"Model-based Identification of Some Conditions Leading to Glycolytic Oscillations in E. coli Cells","authors":"G. Maria, M. Mihalachi, C. Gijiu","doi":"10.15255/CABEQ.2017.1241","DOIUrl":"https://doi.org/10.15255/CABEQ.2017.1241","url":null,"abstract":"Autonomous oscillations of glycolytic intermediate concentrations reflect the dynamics of the control and regulation of this major catabolic pathway, and this phenomenon has been reported in a broad range of bacteria. Understanding glycolytic oscillations might therefore prove crucial for the general understanding of the regulation of cell metabolism with immediate practical applications, allowing in silico design of modified cells with desirable ‘motifs’ of practical applications in the biosynthesis industry, environmental engineering, and medicine. By using a kinetic model from literature, this paper is aiming at in silico (model-based) identification of some conditions leading to the occurrence of stable glycolytic oscillations in the E. coli cells.","PeriodicalId":9765,"journal":{"name":"Chemical and Biochemical Engineering Quarterly","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2019-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42066951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-16DOI: 10.15255/CABEQ.2018.1396
D. Valinger, Matea Kušen, A. Tusek, M. Panić, Tamara Jurina, M. Benković, I. R. Redovniković, J. Kljusurić
{"title":"Development of Near Infrared Spectroscopy Models for Quantitative Prediction of the Content of Bioactive Compounds in Olive Leaves","authors":"D. Valinger, Matea Kušen, A. Tusek, M. Panić, Tamara Jurina, M. Benković, I. R. Redovniković, J. Kljusurić","doi":"10.15255/CABEQ.2018.1396","DOIUrl":"https://doi.org/10.15255/CABEQ.2018.1396","url":null,"abstract":"","PeriodicalId":9765,"journal":{"name":"Chemical and Biochemical Engineering Quarterly","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2019-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.15255/CABEQ.2018.1396","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46993074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-16DOI: 10.15255/CABEQ.2018.1400
Klavdija Zirngast, Z. Kravanja, Z. N. Pintarič
This contribution presents a new robust decomposition methodology for generating optimal flexible process flow sheets with a large number of uncertain parameters. During the initial steps, first-stage variables are determined by performing mixed-integer nonlinear programming (MINLP) synthesis of a flow sheet at the nominal conditions, and then by exposing the obtained flow sheet sequentially over a set of extreme MINLP scenarios of uncertain parameters. As a result, the sizes of the flow-sheet units gradually increase, and/or new units are added until the required feasibility is achieved. After testing the flexibility of the obtained design, a Monte Carlo stochastic optimization of the second-stage variables is performed using a sampling method in order to obtain an optimum value of the expected objective variable. The advantages of the proposed methodology are the independence of process model sizes from the number of uncertain parameters, the straightforward use of deterministic models for incorporating uncertainty, and relatively simple execution of MINLP synthesis of processes under uncertainty. Thus, it could be used for designing large processes with a large number of uncertain parameters. The methodology is illustrated by synthesis of a flexible Heat Exchanger Network.
{"title":"A Robust Decomposition Methodology for Synthesis of Flexible Processes with Many Uncertainty Parameters – Application to HEN Synthesis","authors":"Klavdija Zirngast, Z. Kravanja, Z. N. Pintarič","doi":"10.15255/CABEQ.2018.1400","DOIUrl":"https://doi.org/10.15255/CABEQ.2018.1400","url":null,"abstract":"This contribution presents a new robust decomposition methodology for generating optimal flexible process flow sheets with a large number of uncertain parameters. During the initial steps, first-stage variables are determined by performing mixed-integer nonlinear programming (MINLP) synthesis of a flow sheet at the nominal conditions, and then by exposing the obtained flow sheet sequentially over a set of extreme MINLP scenarios of uncertain parameters. As a result, the sizes of the flow-sheet units gradually increase, and/or new units are added until the required feasibility is achieved. After testing the flexibility of the obtained design, a Monte Carlo stochastic optimization of the second-stage variables is performed using a sampling method in order to obtain an optimum value of the expected objective variable. The advantages of the proposed methodology are the independence of process model sizes from the number of uncertain parameters, the straightforward use of deterministic models for incorporating uncertainty, and relatively simple execution of MINLP synthesis of processes under uncertainty. Thus, it could be used for designing large processes with a large number of uncertain parameters. The methodology is illustrated by synthesis of a flexible Heat Exchanger Network.","PeriodicalId":9765,"journal":{"name":"Chemical and Biochemical Engineering Quarterly","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2019-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47178747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-16DOI: 10.15255/CABEQ.2018.1409
Nenad Marđetko, M. Novak, A. Trontel, M. Grubišić, Maja Galić, B. Šantek
Sustainable recycling of lignocellulosic biomass includes utilization of all carbohydrates present in its hydrolysates. Since wheat straw is a xylose-rich raw material, utilization of xylose from obtained liquid part (liquor) of hydrolysates improves overall bioprocess efficiency. In this work, dilute acid pre-treatment of wheat straw was performed in high-pressure reactor at different temperatures (160 °C – 200 °C), residence times (1 min – 10 min), and acids (H2SO4 and H3PO4) concentrations. During dilute acid pre- treatment, hemicellulose is degraded to pentose sugars that cannot be used by industrial ethanol- producing yeasts. Therefore, genetically engineered Saccharomyces cerevisiae strain that can utilize xylose was used. Fermentations were performed on different xylose-rich liquor wheat straw hydrolysates in shake-flasks and in horizontal rotating tubular bioreactor. The efficiency of fermentations carried out in shake flasks using xylose- rich liquor wheat straw hydrolysates were in the range of 19.61 – 74.51 %. However, the maximum bioprocess efficiency (88.24 %) was observed during fermentation in the HRTB on the liquor wheat straw hydrolysate obtained by pre- treatment with 2 % w/w phosphoric acid.
{"title":"Bioethanol Production from Dilute-acid Pre-treated Wheat Straw Liquor Hydrolysate by Genetically Engineered Saccharomyces cerevisiae","authors":"Nenad Marđetko, M. Novak, A. Trontel, M. Grubišić, Maja Galić, B. Šantek","doi":"10.15255/CABEQ.2018.1409","DOIUrl":"https://doi.org/10.15255/CABEQ.2018.1409","url":null,"abstract":"Sustainable recycling of lignocellulosic biomass includes utilization of all carbohydrates present in its hydrolysates. Since wheat straw is a xylose-rich raw material, utilization of xylose from obtained liquid part (liquor) of hydrolysates improves overall bioprocess efficiency. In this work, dilute acid pre-treatment of wheat straw was performed in high-pressure reactor at different temperatures (160 °C – 200 °C), residence times (1 min – 10 min), and acids (H2SO4 and H3PO4) concentrations. During dilute acid pre- treatment, hemicellulose is degraded to pentose sugars that cannot be used by industrial ethanol- producing yeasts. Therefore, genetically engineered Saccharomyces cerevisiae strain that can utilize xylose was used. Fermentations were performed on different xylose-rich liquor wheat straw hydrolysates in shake-flasks and in horizontal rotating tubular bioreactor. The efficiency of fermentations carried out in shake flasks using xylose- rich liquor wheat straw hydrolysates were in the range of 19.61 – 74.51 %. However, the maximum bioprocess efficiency (88.24 %) was observed during fermentation in the HRTB on the liquor wheat straw hydrolysate obtained by pre- treatment with 2 % w/w phosphoric acid.","PeriodicalId":9765,"journal":{"name":"Chemical and Biochemical Engineering Quarterly","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2019-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.15255/CABEQ.2018.1409","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48451242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-16DOI: 10.15255/CABEQ.2018.1371
M. Berovič, M. Popović
European isolated strain Ganoderma lucidum, its biomass formation, intracellular and extracellular polysaccharide production in batch and repeated fedbatch cultivation, were studied. The best fed batch results were obtained in the first feeding at 141 h at productivity of biomass Px = 0.016 g g–1 h–1, intracellular Pi = 0.010 g g–1 h–1, extracellular polysaccharides Pe = 0.007 g g–1 h–1, and percent value of polysaccharides per cell dry weight Pi/x = 5.0 % and Pe/x = 3.0 %. Although four feedings increased the amount of fungal biomass by 42.66 %, productivities of fungal polysaccharides Pi = 0.010 g g–1 L–1 and Pe = 0.011 g g–1 L–1 showed that at the fourth feeding, the biomass was already exhausted and polysaccharide production was reduced. Therefore, 210 h batch cultivation with Px = 0.095 g g–1 L–1, Pi = 0.078 g g–1 L–1 at Pi/x = 33 % and extracellular polysaccharides Pe = 0.095 g g–1 L–1 at Pe/x = 6.8 %, was found to be the best mode for G. lucidum fungal biomass and polysaccharides production. Non-Newtonian cultivation broth rheology was found to be influenced entirely by the concentration and morphology of the biomass.
研究了欧洲分离菌株灵芝的生物量形成、细胞内和细胞外多糖的分批培养和重复补料培养。在141小时的第一次进料中获得了最佳的补料分批结果，生产力为生物质Px=0.016 g g–1 h–1，细胞内Pi=0.010 g g–2 h–1、细胞外多糖Pe=0.007 g g–3 h–1以及每细胞干重多糖百分比Pi/x=5.0%和Pe/x=3.0%。尽管四次饲养使真菌生物量增加了42.66%，但真菌多糖的产量Pi=0.010 g g–1 L–1和Pe=0.011 g g–L L–1表明，在第四次饲养时，生物量已经耗尽，多糖产量减少。因此，发现210小时分批培养是灵芝真菌生物量和多糖生产的最佳模式，其中Px=0.095 g g–1 L–1，Pi/x=33%时Pi=0.078 g g–1L–1，Pe/x=6.8%时胞外多糖Pe=0.095 g g–1l–1。发现非牛顿培养基的流变性完全受生物质的浓度和形态的影响。
{"title":"Submerged Cultivation of Ganoderma lucidum Biomass in Stirred Tank Reactor","authors":"M. Berovič, M. Popović","doi":"10.15255/CABEQ.2018.1371","DOIUrl":"https://doi.org/10.15255/CABEQ.2018.1371","url":null,"abstract":"European isolated strain Ganoderma lucidum, its biomass formation, intracellular and extracellular polysaccharide production in batch and repeated fedbatch cultivation, were studied. The best fed batch results were obtained in the first feeding at 141 h at productivity of biomass Px = 0.016 g g–1 h–1, intracellular Pi = 0.010 g g–1 h–1, extracellular polysaccharides Pe = 0.007 g g–1 h–1, and percent value of polysaccharides per cell dry weight Pi/x = 5.0 % and Pe/x = 3.0 %. Although four feedings increased the amount of fungal biomass by 42.66 %, productivities of fungal polysaccharides Pi = 0.010 g g–1 L–1 and Pe = 0.011 g g–1 L–1 showed that at the fourth feeding, the biomass was already exhausted and polysaccharide production was reduced. Therefore, 210 h batch cultivation with Px = 0.095 g g–1 L–1, Pi = 0.078 g g–1 L–1 at Pi/x = 33 % and extracellular polysaccharides Pe = 0.095 g g–1 L–1 at Pe/x = 6.8 %, was found to be the best mode for G. lucidum fungal biomass and polysaccharides production. Non-Newtonian cultivation broth rheology was found to be influenced entirely by the concentration and morphology of the biomass.","PeriodicalId":9765,"journal":{"name":"Chemical and Biochemical Engineering Quarterly","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2019-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46159225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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