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Involvement of PDGFR-integrin interactions in the regulation of anoikis resistance in glioblastoma progression 表皮生长因子受体-整合素相互作用参与调控胶质母细胞瘤进展过程中的阿诺伊克抗性。
IF 3.3 3区 生物学 Q3 CELL BIOLOGY Pub Date : 2024-11-10 DOI: 10.1002/cbin.12257
Pampa Pain, Ashutosh Tripathi, Prakash P. Pillai

The interactions between platelet-derived growth factor/PDGF receptor and integrin signaling are crucial for cells to respond to extracellular stimuli. Integrin interactions with PDGFR within the lipid rafts activate downstream cellular signaling pathways that regulate cell proliferation, cell migration, cell differentiation, and cell death processes. The mechanisms underlying PDGFR activation mediated receptor internalization, interactions with other cell-surface receptors, particularly extracellular matrix receptors, integrins, and how these cellular mechanisms switch on anchorage-independent cell survival, leading to anoikis resistance are discussed. The role of regulatory molecules such as noncoding RNAs, that can modulate several molecular and cellular processes, including autophagy, in the acquisition of anoikis resistance is also discussed. Overall, the review provides a new perspective on a complex interplay of regulatory cellular machineries involving autophagy, noncoding RNAs and cellular mechanisms of PDGFR activation, PDGFR-integrin interactions, and involvement of lipids rafts in the acquisition of anoikis resistance that regulates glioblastoma progression along with potential future strategies to develop novel therapeutics for glioblastoma multiforme.

血小板衍生生长因子/PDGF 受体与整合素信号之间的相互作用对于细胞响应细胞外刺激至关重要。整合素与脂质筏中的 PDGFR 相互作用激活了下游细胞信号通路,从而调节细胞增殖、细胞迁移、细胞分化和细胞死亡过程。本文讨论了 PDGFR 激活介导的受体内化、与其他细胞表面受体(尤其是细胞外基质受体、整合素)相互作用的机制,以及这些细胞机制如何开启不依赖锚定的细胞存活,从而导致抗厌氧菌性。此外,还讨论了非编码 RNA 等调控分子在获得 anoikis 抗性过程中的作用,它们可以调节自噬等多个分子和细胞过程。总之,这篇综述提供了一个新的视角,揭示了自噬、非编码 RNA、PDGFR 激活的细胞机制、PDGFR-整合素相互作用以及脂质筏的参与等细胞调控机制在获取 anoikis 抗性、调控胶质母细胞瘤进展过程中的复杂相互作用,以及开发治疗多形性胶质母细胞瘤新型疗法的潜在未来策略。
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引用次数: 0
Carnosol alleviates cisplatin–induced acute kidney injury by regulating apoptosis and pyroptosis 卡诺索尔通过调节细胞凋亡和热解减轻顺铂诱导的急性肾损伤
IF 3.3 3区 生物学 Q3 CELL BIOLOGY Pub Date : 2024-11-10 DOI: 10.1002/cbin.12258
Chunjie Li, Hongyan Yang, Yuan Wu, Mingke Zhou, Hengbiao Luo, Peng Yuan, Fengge Shen

The use of the common anticancer drug cisplatin (CP) in clinical practice often leads to acute kidney injury (AKI); however, no protective therapy is available. Therefore, new drugs that reduce the nephrotoxicity induced by CP are urgently needed. Carnosol (CA) is an antioxidant found. We investigated the renoprotective effects of CA on CP-induced AKI in male C57BL/6 mice and HK2 cells. CA mitigated renal dysfunction, histopathological changes and tubular injury in vivo, as indicated by the expression of NGAL, KIM1 and HMGB1. Moreover, the numbers of apoptotic cells and the expression of apoptotic proteins were dramatically reduced after CA treatment in mouse kidneys and HK2 cells. CA significantly ameliorated CP-induced inflammation and decreased TNF-α and IL-1β levels in vivo and in vitro and macrophage infiltration in the mouse kidney. CA decreased the expression levels of p-p65/p65, NLRP3 and ASC, which indicates that CA suppressed the activation of the NF-κB/NLRP3 signaling axis induced by CP in vivo and in vitro. In addition, CA decreased the levels of certain protein in pyroptotic cells, as indicated by the expression of cleaved caspase-1, GSDMD, and mature IL-1β and IL-18 in vivo and in vitro. Finally, CA reduced the level of cleaved caspase-1, but those of GSDMD and NLRP3 protein were not significantly different after treatment with the NLRP3 inhibitor MCC950 and were elevated by the NLRP3 activator nigericin. In conclusion, this study revealed that CA protects against CP-induced AKI by decreasing apoptosis and NF-κB/NLRP3/GSDMD-mediated pyroptosis, which provides new insight into the prevention of AKI.

在临床实践中,使用常见的抗癌药物顺铂(CP)常常会导致急性肾损伤(AKI);然而,目前还没有保护性疗法。因此,迫切需要能降低顺铂引起的肾毒性的新药。卡诺索尔(Carnosol,CA)是一种抗氧化剂。我们在雄性 C57BL/6 小鼠和 HK2 细胞中研究了 CA 对氯化石蜡诱导的 AKI 的肾保护作用。从 NGAL、KIM1 和 HMGB1 的表达来看,CA 可减轻体内肾功能障碍、组织病理变化和肾小管损伤。此外,小鼠肾脏和 HK2 细胞经 CA 处理后,凋亡细胞的数量和凋亡蛋白的表达均显著减少。CA能明显改善CP诱导的炎症反应,降低体内和体外TNF-α和IL-1β的水平以及小鼠肾脏中巨噬细胞的浸润。CA降低了p-p65/p65、NLRP3和ASC的表达水平,这表明CA抑制了体内和体外CP诱导的NF-κB/NLRP3信号轴的激活。此外,CA 还降低了脓细胞中某些蛋白质的水平,体内和体外的裂解 Caspase-1、GSDMD 以及成熟 IL-1β 和 IL-18 的表达均表明了这一点。最后,CA降低了裂解的caspase-1的水平,但GSDMD和NLRP3蛋白的水平在NLRP3抑制剂MCC950处理后无明显差异,而在NLRP3激活剂尼格列汀处理后则升高。总之,本研究揭示了CA通过减少细胞凋亡和NF-κB/NLRP3/GSDMD介导的热蛋白沉积对CP诱导的AKI具有保护作用,这为预防AKI提供了新的思路。
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引用次数: 0
Correction to “The Hippo–YAP pathway regulates the proliferation of alveolar epithelial progenitors after acute lung injury” 对 "急性肺损伤后肺泡上皮祖细胞的增殖受 Hippo-YAP 通路调控 "的更正。
IF 3.3 3区 生物学 Q3 CELL BIOLOGY Pub Date : 2024-11-06 DOI: 10.1002/cbin.12247

Hu C, Sun J, Du J, Wen D, Lu H, Zhang H, Xue Y, Zhang A, Yang C, Zeng L, Jiang J. The Hippo-YAP pathway regulates the proliferation of alveolar epithelial progenitors after acute lung injury. Cell Biol Int. 2019;43(10):1174-1183. https://doi.org/10.1002/cbin.11098

We regret to acknowledge a nonintentional error in Figure 4 C. The light microscopy image of the verteporfin-48h group was inaccurate in the figure. We, therefore, corrected it.

Updated Figure 4 is included below:

Hu C, Sun J, Du J, Wen D, Lu H, Zhang H, Xue Y, Zhang A, Yang C, Zeng L, Jiang J. The Hippo-YAP pathway regulates the proliferation of alveolar epithelial progenitors after acute lung injury.Cell Biol Int. 2019;43(10):1174-1183。https://doi.org/10.1002/cbin.11098We,很遗憾在图 4 C 中出现了一个非故意的错误。图中verteporfin-48h组的光镜图像不准确。因此,我们对其进行了更正。更新后的图 4 包含在下面:
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引用次数: 0
ZNF655 involved in the progression of multiple myeloma via the activation of AKT. ZNF655 通过激活 AKT 参与多发性骨髓瘤的进展。
IF 3.3 3区 生物学 Q3 CELL BIOLOGY Pub Date : 2024-11-03 DOI: 10.1002/cbin.12256
Haiming Kou, Shuqin Jiang, Xueqiong Wu, Changhua Jing, Xinxin Xu, Jiaju Wang, Cui Zhang, Wenting Liu, Yan Gao, Qian Men, Ping Lu, Zhenhui Lv

Multiple myeloma (MM) is an incurable hematological malignancy, and the number of MM patients is increasing year by year. Zinc finger protein 655 (ZNF655) has been shown to regulate various biological processes and is implicated in the progression of many diseases. However, the roles of ZNF655 in MM progression remains unclear. In this study, we aimed to explore the effects of ZNF655 on progression by detecting the alteration of the phenotypes and tumorigenesis induced by ZNF655 knockdown in MM. The expression level of ZNF655 in MM was clarified by real-time quantitative polymerase chain reaction assays. Furthermore, loss-of-function assays in vitro and in vivo was investigated the biological functions of ZNF655 in MM. These findings revealed that ZNF655 depletion remarkably inhibited MM cell proliferation, arrested cell cycle, and induced cell apoptosis. Mechanistically, ZNF655 was found to regulate AKT in MM. In conclusion, this study indicated that ZNF655 regulated the progression of MM via AKT activation and downregulation of ZNF655 may be a promising antitumor strategy in MM.

多发性骨髓瘤(MM)是一种无法治愈的血液系统恶性肿瘤,MM 患者人数逐年增加。锌指蛋白655(ZNF655)已被证明能调节多种生物过程,并与多种疾病的进展有关。然而,ZNF655在MM进展中的作用仍不清楚。在本研究中,我们旨在通过检测ZNF655敲除在MM中诱导的表型和肿瘤发生的改变来探索ZNF655对进展的影响。本研究通过实时定量聚合酶链反应测定明确了ZNF655在MM中的表达水平。此外,还通过体外和体内功能缺失实验研究了ZNF655在MM中的生物学功能。这些研究结果表明,ZNF655 的缺失能显著抑制 MM 细胞的增殖、抑制细胞周期并诱导细胞凋亡。从机理上讲,ZNF655 可调控 MM 中的 AKT。总之,这项研究表明,ZNF655通过激活AKT调控MM的进展,而下调ZNF655可能是治疗MM的一种有前景的抗肿瘤策略。
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引用次数: 0
MARCHF1 promotes breast cancer through accelerating REST ubiquitylation and following TFAM transcription. MARCHF1 通过加速 REST 泛素化和跟随 TFAM 转录来促进乳腺癌的发生。
IF 3.3 3区 生物学 Q3 CELL BIOLOGY Pub Date : 2024-10-20 DOI: 10.1002/cbin.12255
Jutao Li, Zhenming Gao

Breast cancer has become the leading cause of death in women. Membrane associated ring-CH-type finger 1 (MARCHF1) is associated with the development of various types of cancer, but the exact role of MARCHF1 in breast cancer remains unclear. In our study, the higher MARCHF1 expression was observed in tumor samples of patients with breast cancer and then the role of MARCHF1 in breast cancer was further evaluated. Overexpression of MARCHF1 contributed to proliferation of cancer cells and inhibition of oxidative stress. Knockdown of MARCHF1 reduced breast cancer cell proliferation, increased mitochondrial dysfunction induced by oxidative stress, eventually aggravating cell death. In vivo, MARCHF1 promoted the tumor growth and oppositely, MARCHF1 silencing suppressed the tumor development. Moreover, MARCHF1 interacted with repressor Element-1 silencing transcription factor (REST) and facilitated its ubiquitylation and degradation. Subsequently, REST negatively regulated the transcription of mitochondrial transcription factor A (TFAM). The subcutaneous tumor formation assay in nude mice also supported these conclusions. In details, knockdown of MARCHF1 upregulated the protein expression of REST and downregulated the mRNA level of TFAM. On the contrary, MARCHF1 overexpression exhibited opposite effects. Thus, MARCHF1 is conducive to the progression of breast cancer via promoting the ubiquitylation and degradation of RSET and then the transcription of TFAM. Downregulating MARCHF1 could provide a novel direction for treating breast cancer.

乳腺癌已成为女性死亡的主要原因。膜相关环CH型手指1(MARCHF1)与多种癌症的发生有关,但MARCHF1在乳腺癌中的确切作用仍不清楚。我们的研究在乳腺癌患者的肿瘤样本中观察到了较高的 MARCHF1 表达,然后进一步评估了 MARCHF1 在乳腺癌中的作用。MARCHF1 的过表达有助于癌细胞的增殖和抑制氧化应激。敲除 MARCHF1 会减少乳腺癌细胞的增殖,增加氧化应激引起的线粒体功能障碍,最终加剧细胞死亡。在体内,MARCHF1促进肿瘤生长,相反,MARCHF1沉默则抑制肿瘤发展。此外,MARCHF1 与抑制因子 Element-1 沉默转录因子(REST)相互作用,促进其泛素化和降解。随后,REST 负向调节线粒体转录因子 A(TFAM)的转录。裸鼠皮下肿瘤形成试验也支持上述结论。具体而言,敲除 MARCHF1 会上调 REST 的蛋白表达,下调 TFAM 的 mRNA 水平。相反,MARCHF1的过表达则表现出相反的效果。因此,MARCHF1通过促进RSET的泛素化和降解,进而促进TFAM的转录,有利于乳腺癌的进展。下调 MARCHF1 可为治疗乳腺癌提供一个新的方向。
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引用次数: 0
Expression of concern: “CXCR4 positive cell-derived Pdx1-high/Shh-low cells originated from embryonic stem cells improve the repair of pancreatic injury in mice” 表达关切:"源自胚胎干细胞的 CXCR4 阳性细胞衍生 Pdx1 高/Shh 低细胞可改善小鼠胰腺损伤的修复"。
IF 3.3 3区 生物学 Q3 CELL BIOLOGY Pub Date : 2024-10-17 DOI: 10.1002/cbin.12254

Expression of Concern: T. Yu, Q. Qing, N. Deng, X.-H. Min, L.-N. Zhao, J.-Y. Li, Z.-S. Xia and Q.-k. Chen. (2015) CXCR4 positive cell-derived Pdx1-high/Shh-low cells originated from embryonic stem cells improve the repair of pancreatic injury in mice. Cell Biology International, 39(9), 995–1006. https://doi.org/10.1002/cbin.10470.

This Expression of Concern for the above article published online on 26 March 2015 in Wiley Online Library (wileyonlinelibrary.com), has been published by agreement between the journal Editor-in-Chief, Xuebiao Yao; International Federation for Cell Biology; and John Wiley & Sons Ltd. The Expression of Concern has been agreed following an investigation conducted by the first author's institution. Evidence of splicing in the western blots presented in Figures 2f, 3b,e and unexpected similarity between western blot bands in Figures 2f and 3e was observed. The first author admitted that western blots were spliced due to limitations in laboratory equipment available at the time. The authors did not provide an adequate explanation for the similarities observed between the western blot bands in Figures 2f and 3e. Due to the length of time that has elapsed since this article was published, the raw data is not available. The journal has decided to issue an Expression of Concern to alert the readers.

表达关切:T. Yu, Q. Qing, N. Deng, X.-H. Min, L.-N.Min, L.-N. Zhao, J.-Y.Zhao, J.-Y. Li, Z.-S.Li, Z.-S.Xia 和 Q.-k. Chen.Chen.(2015)源自胚胎干细胞的CXCR4阳性细胞源Pdx1-高/Shh-低细胞改善了小鼠胰腺损伤的修复。Cell Biology International, 39(9), 995-1006. https://doi.org/10.1002/cbin.10470.This 上述文章于 2015 年 3 月 26 日在线发表于 Wiley Online Library (wileyonlinelibrary.com),其关注声明已由期刊主编 Xuebiao Yao、国际细胞生物学联合会(International Federation for Cell Biology)和 John Wiley & Sons Ltd.(John Wiley & Sons Ltd.)协议发表。在第一作者所在机构进行调查后,同意发表该《关注表达》。在图 2f、3b、e 中的 Western 印迹中发现了拼接的证据,图 2f 和图 3e 中的 Western 印迹条带之间出现了意想不到的相似性。第一作者承认,由于当时实验室设备的限制,对 Western 印迹进行了拼接。作者没有对图 2f 和图 3e 中观察到的 western 印迹条带之间的相似性做出适当解释。由于这篇文章发表已过了很长时间,因此无法获得原始数据。本刊决定发布 "关注函",以提醒读者注意。
{"title":"Expression of concern: “CXCR4 positive cell-derived Pdx1-high/Shh-low cells originated from embryonic stem cells improve the repair of pancreatic injury in mice”","authors":"","doi":"10.1002/cbin.12254","DOIUrl":"10.1002/cbin.12254","url":null,"abstract":"<p><b>Expression of Concern</b>: T. Yu, Q. Qing, N. Deng, X.-H. Min, L.-N. Zhao, J.-Y. Li, Z.-S. Xia and Q.-k. Chen. (2015) CXCR4 positive cell-derived Pdx1-high/Shh-low cells originated from embryonic stem cells improve the repair of pancreatic injury in mice. <i>Cell Biology International</i>, 39(9), 995–1006. https://doi.org/10.1002/cbin.10470.</p><p>This Expression of Concern for the above article published online on 26 March 2015 in Wiley Online Library (wileyonlinelibrary.com), has been published by agreement between the journal Editor-in-Chief, Xuebiao Yao; International Federation for Cell Biology; and John Wiley &amp; Sons Ltd. The Expression of Concern has been agreed following an investigation conducted by the first author's institution. Evidence of splicing in the western blots presented in Figures 2f, 3b,e and unexpected similarity between western blot bands in Figures 2f and 3e was observed. The first author admitted that western blots were spliced due to limitations in laboratory equipment available at the time. The authors did not provide an adequate explanation for the similarities observed between the western blot bands in Figures 2f and 3e. Due to the length of time that has elapsed since this article was published, the raw data is not available. The journal has decided to issue an Expression of Concern to alert the readers.</p>","PeriodicalId":9806,"journal":{"name":"Cell Biology International","volume":"48 12","pages":"1906"},"PeriodicalIF":3.3,"publicationDate":"2024-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cbin.12254","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142459182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Rotigaptide inhibits spontaneous contractions of gastric smooth muscle in diabetic rats via the PKCα-Cx43 pathway 罗替加肽通过 PKCα-Cx43 途径抑制糖尿病大鼠胃平滑肌的自发收缩
IF 3.3 3区 生物学 Q3 CELL BIOLOGY Pub Date : 2024-10-13 DOI: 10.1002/cbin.12253
Lu Changri, Haibei Sun, Yitegele Bao, Mohan Zhang

The study aimed to investigate the effect of rotigaptide (ZP123) on spontaneous contractions of gastric smooth muscle in diabetic rats and explore the underlying mechanisms. Twelve rats were randomly divided into model and normal control groups. Changes in gastric smooth muscle spontaneous contractions in each group were observed. Western blot analysis was performed to detect Cx43 and PKCα expression. Rat gastric smooth muscle cells were cultured in vitro and divided into normal glucose, high glucose and high glucose+rotigaptide group. The intracellular Ca2+ content was observed by immunofluorescence. The amplitude and frequency of gastric smooth muscle spontaneous contractions were reduced in the model group than the normal control group (all p < .01), which were reduced after rotigatide treatment than before treatment in the model group (all p < .01). The model+rotigaptide group showed decreased membrane expression of Cx43, increased cytoplasmic expression of Cx43, increased membrane expression of p-PKCα Thr497 and lower membrane/cytoplasm ratio of Cx43 expression compared with the model group (all p < .01). The intracellular Ca2+ content was increased in the high glucose group than the normal glucose group (p < .01), while no significant difference was observed between the high glucose+rotigaptide and high glucose groups. Our findings suggest that rotigatide can stabilize the intracellular Ca2+ concentration in gastric smooth muscle cells under high glucose condition by upregulating PKCα activity and downregulating the number of GJs and the opening rate of GJ hemichannels through the PKCα-Cx43 pathway, thus inhibiting spontaneous contractions of gastric smooth muscle in diabetic rats.

本研究旨在探讨罗替加肽(ZP123)对糖尿病大鼠胃平滑肌自发性收缩的影响及其内在机制。研究将 12 只大鼠随机分为模型组和正常对照组。观察各组胃平滑肌自发收缩的变化。用 Western 印迹分析检测 Cx43 和 PKCα 的表达。体外培养大鼠胃平滑肌细胞,将其分为正常葡萄糖组、高葡萄糖组和高葡萄糖+罗格列肽组。免疫荧光法观察细胞内 Ca2+ 的含量。与正常对照组相比,模型组胃平滑肌自发收缩的幅度和频率降低(所有 p 497 和 Cx43 表达的膜/胞浆比值降低);与正常对照组相比,模型组胃平滑肌自发收缩的幅度和频率升高(所有 p 2+ 含量升高);与正常对照组相比,高糖组胃平滑肌自发收缩的幅度和频率升高(所有 p 2+ 含量升高)、从而抑制糖尿病大鼠胃平滑肌的自发收缩。
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引用次数: 0
Oxidative stress disrupts vascular microenvironmental homeostasis affecting the development of atherosclerosis 氧化应激破坏血管微环境稳态,影响动脉粥样硬化的发展。
IF 3.3 3区 生物学 Q3 CELL BIOLOGY Pub Date : 2024-10-06 DOI: 10.1002/cbin.12239
Ruifei Shao, Rui Chen, Qiang Zheng, Mengyu Yao, Kunlin Li, Yu Cao, Lihong Jiang

Atherosclerosis is primarily an inflammatory reaction of the cardiovascular system caused by endothelial damage, leading to progressive thickening and hardening of the vessel walls, as well as extensive necrosis and fibrosis of the surrounding tissues, the most necessary pathological process causing cardiovascular disease. When the body responds to harmful internal and external stimuli, excess oxygen free radicals are produced causing oxidative stress to occur in cells and tissues. Simultaneously, the activation of inflammatory immunological processes is followed by an elevation in oxygen free radicals, which directly initiates the release of cytokines and chemokines, resulting in a detrimental cycle of vascular homeostasis abnormalities. Oxidative stress contributes to the harm inflicted upon vascular endothelial cells and the decrease in nitric oxide levels. Nitric oxide is crucial for maintaining vascular homeostasis and is implicated in the development of atherosclerosis. This study examines the influence of oxidative stress on the formation of atherosclerosis, which is facilitated by the vascular milieu. It also provides an overview of the pertinent targets and pharmaceutical approaches for treating this condition.

动脉粥样硬化主要是内皮损伤引起的心血管系统炎症反应,导致血管壁逐渐增厚和硬化,以及周围组织广泛坏死和纤维化,是引起心血管疾病最必要的病理过程。当人体对有害的内外刺激做出反应时,会产生过量的氧自由基,导致细胞和组织发生氧化应激。与此同时,炎症免疫过程被激活,氧自由基随之升高,直接引发细胞因子和趋化因子的释放,导致血管稳态异常的有害循环。氧化应激对血管内皮细胞造成伤害,并导致一氧化氮水平下降。一氧化氮对维持血管稳态至关重要,并与动脉粥样硬化的发展有关。本研究探讨了氧化应激对动脉粥样硬化形成的影响,而血管环境有助于动脉粥样硬化的形成。它还概述了治疗这种疾病的相关靶点和药物方法。
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引用次数: 0
Flurbiprofen axetil is involved in basal-like breast cancer metastasis via suppressing the MEK/ERK signaling pathway 氟比洛芬酯通过抑制MEK/ERK信号通路参与基底样乳腺癌的转移。
IF 3.3 3区 生物学 Q3 CELL BIOLOGY Pub Date : 2024-10-04 DOI: 10.1002/cbin.12251
Yalin Zhu, Yi Gong, Yifei Wang, Zhengyu Jiang, Ying Yao, Xiaoyong Miao, Shuoer Wang, Yan Zhang, Jianping Cao

Flurbiprofen axetil is commonly utilized in clinical practice as one of the nonsteroidal anti-inflammatory drugs (NSAIDs) and is included in multimodal analgesia regimens postbreast cancer surgery. Numerous NSAIDs have been studied for their potential to both promote and inhibit cancer. Given the variability in their effects on tumors, further investigation into the specific role of flurbiprofen axetil is warranted. Therefore, the primary objective of this study was to assess the impact of flurbiprofen axetil on basal-like breast cancer (BLBC) metastasis and elucidate the underlying molecular mechanisms involved. The BLBC metastasis mouse model was established by caudal vein injection of tumor cells. The lung metastasis of breast cancer in mice and the effect of flurbiprofen axetil were assessed by in vivo bioluminescence imaging, hematoxylin and eosin staining and immunohistochemistry. In vitro, the results of flurbiprofen axetil on the proliferation, migration, and invasion of MDA-MB-231 human breast cancer cells and BT-549 human breast cancer cells were assessed by colony formation assay and transwell assay. The effects of flurbiprofen axetil on several tumor metastasis-related signaling pathway proteins were examined by western blot, and the reversal extent of the flurbiprofen axetil effect by Ro 67-7476 (ERK phosphorylation agonist) was detected by transwell assay. The results showed that flurbiprofen axetil significantly inhibited BLBC lung metastasis in mice. Flurbiprofen axetil similarly inhibited breast cancer cell migration and invasion in vitro but did not affect their proliferation. Mechanistic investigations have revealed that flurbiprofen axetil exerts a noteworthy inhibitory influence on the MEK/ERK pathway while exhibiting no significant alteration in the expression of other pathway proteins intricately associated with epithelial–mesenchymal transition. In conclusion, the inhibitory effect of flurbiprofen axetil on BLBC metastasis is characterized by its selectivity in targeting the MEK/ERK signaling pathway rather than exerting a broad impact on the global signaling pathway.

氟比洛芬酯是临床上常用的非甾体抗炎药(NSAIDs)之一,并被纳入乳腺癌术后多模式镇痛方案。许多非甾体抗炎药都有促进和抑制癌症的作用。鉴于非甾体抗炎药对肿瘤的影响各不相同,有必要进一步研究氟比洛芬酯的具体作用。因此,本研究的主要目的是评估氟比洛芬酯对基底样乳腺癌(BLBC)转移的影响,并阐明其潜在的分子机制。通过尾静脉注射肿瘤细胞建立了基底样乳腺癌转移小鼠模型。通过体内生物发光成像、苏木精和伊红染色以及免疫组织化学方法评估了乳腺癌小鼠的肺转移情况以及氟比洛芬 axetil 的作用。在体外,通过集落形成试验和透孔试验评估了氟比洛芬酯对 MDA-MB-231 人乳腺癌细胞和 BT-549 人乳腺癌细胞增殖、迁移和侵袭的影响。通过Western印迹检测了氟比洛芬对几种肿瘤转移相关信号通路蛋白的影响,并通过透孔试验检测了Ro 67-7476(ERK磷酸化激动剂)对氟比洛芬作用的逆转程度。结果表明,氟比洛芬 axetil 能显著抑制小鼠 BLBC 肺转移。氟比洛芬酯同样能抑制乳腺癌细胞在体外的迁移和侵袭,但不影响其增殖。机理研究发现,氟比洛芬 axetil 对 MEK/ERK 通路具有显著的抑制作用,而对与上皮-间质转化密切相关的其他通路蛋白的表达没有明显改变。总之,氟比洛芬 axetil 对 BLBC 转移的抑制作用的特点是其针对 MEK/ERK 信号通路的选择性,而不是对整个信号通路产生广泛的影响。
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引用次数: 0
Role of SEL1L in the progression of solid tumors, with a special focus on its recent therapeutic potential SEL1L 在实体瘤进展过程中的作用,特别关注其近期的治疗潜力。
IF 3.3 3区 生物学 Q3 CELL BIOLOGY Pub Date : 2024-10-04 DOI: 10.1002/cbin.12242
Darmadi Darmadi, Raed Obaid Saleh, Enwa Felix Oghenemaro, Maha Noori Shakir, Ahmed Hjazi, Zahraa F. Hassan, Ahmed Hussein Zwamel, Sanoeva Matlyuba, Mahamedha Deorari, Shamam Kareem Oudah

Since suppressor/enhancer of Lin-12-like (SEL1L) was cloned in 1997, various pieces of evidence from lower species suggest it plays a significant role in protein degradation via the ubiquitin-proteasome system. The relevance of SEL1L in many aspects of malignant transformation and tumorigenic events has been the subject of research, which has shown compelling in vitro and in vivo findings relating its altered expression to changes in tumor aggressiveness. The Endoplasmic Reticulum (ER) in tumor cells is crucial for preserving cellular proteostasis by inducing the unfolded protein response (UPR), a stress response. A crucial component of the UPR is ER-associated degradation (ERAD), which guards against ER stress-induced apoptosis and the removal of unfolded or misfolded proteins by the ubiquitin-proteasome system. As a protein stabilizer of HMG-CoA reductase degradation protein 1 (HRD1), one of the main components of ERAD, SEL1L plays an important role in ER homeostasis. Notably, the expression levels of these two proteins fluctuate independently in various cancer types, yet changes in their expression affect the levels of other associated proteins during cancer pathogenesis. Recent studies have also outlined the function of SEL1L in cancer medication resistance. This review explores the value of targeting SEL1L as a novel treatment approach for cancer, focusing on the molecular processes of SEL1L and its involvement in cancer etiology.

自1997年克隆出Lin-12-样抑制因子/增强因子(SEL1L)以来,来自低等物种的各种证据表明,它通过泛素-蛋白酶体系统在蛋白质降解过程中发挥着重要作用。SEL1L 在恶性转化和致瘤事件的许多方面的相关性一直是研究的主题,其体外和体内研究结果令人信服地表明,SEL1L 的表达改变与肿瘤侵袭性的变化有关。肿瘤细胞中的内质网(ER)是通过诱导未折叠蛋白反应(UPR)这一应激反应来维持细胞蛋白稳态的关键。UPR的一个重要组成部分是ER相关降解(ERAD),它可防止ER应激诱导的细胞凋亡,并通过泛素-蛋白酶体系统清除未折叠或折叠错误的蛋白质。作为ERAD的主要成分之一--HMG-CoA还原酶降解蛋白1(HRD1)的蛋白稳定剂,SEL1L在ER平衡中发挥着重要作用。值得注意的是,这两种蛋白的表达水平在各种癌症类型中独立波动,但它们的表达变化会影响癌症发病过程中其他相关蛋白的水平。最近的研究还概述了 SEL1L 在癌症耐药性中的功能。本综述探讨了靶向 SEL1L 作为一种新型癌症治疗方法的价值,重点是 SEL1L 的分子过程及其在癌症病因学中的参与。
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引用次数: 0
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Cell Biology International
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