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Impact of deep eutectic solvent deamidation on the solubility and conformation of rice dreg protein isolate
IF 2.2 4区 农林科学 Q3 CHEMISTRY, APPLIED Pub Date : 2024-11-08 DOI: 10.1002/cche.10848
Asifa Shahid, Muhammad Abdul Haq, Naveed Hussain, Haq Nawaz, Jiaxing Xu

Background and Objectives

Rice dreg protein isolate (RDPI) is identified as a sustainable protein source with promising applications in the food and pharmaceutical industries. However, its utility is limited by poor solubility and restricted conformational stability. The objective of this study is to explore how natural deep eutectic solvent (NADES) deamidation can enhance the solubility and structural properties of RDPI, making it more suitable for various applications.

Findings

The study employed a two-step process to obtain RDPI via isoelectric precipitation, followed by deamidation using an NADES made from glucose and citric acid. The deamidation process significantly improved RDPI's solubility, which enhanced its dispersibility in aqueous systems. Additionally, deamidated RDPI exhibited enhanced foaming and emulsion capacities, indicating improved functional properties due to increased structural flexibility.

Conclusions

The deamidation of RDPI using NADES effectively enhances both its solubility and functional properties, making it a more versatile ingredient for food and pharmaceutical applications. The improved structural flexibility achieved through deamidation is key to these enhancements.

Significance and Novelty

This research introduces NADES deamidation as a promising, sustainable approach for improving the solubility, conformational properties, and functional performance of RDPI. The findings provide valuable insights for the development of protein-based ingredients with enhanced functionality, broadening their application potential in various industries.

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引用次数: 0
Comparative analysis of different carrier agents on functional, structural, and thermal properties of spray-dried probiotic Lactobacillus casei powder
IF 2.2 4区 农林科学 Q3 CHEMISTRY, APPLIED Pub Date : 2024-11-07 DOI: 10.1002/cche.10842
Poorva Sharma, Michael T. Nickerson, Darren R. Korber

Background and Objectives

The aim of this study was to investigate the effect of incorporation of different plant-based polysaccharides (pectin, maltodextrin (MD) and gum arabic (GA)) with pea protein isolate (PPI) to obtain maximum encapsulation efficiency (EE), gastrointestinal (GI) stability and yield of probiotic Lactobacillus casei through spray drying. Several characteristics of encapsulated vegan probiotic powders were evaluated including functional, structural, and thermal characteristics.

Findings

The results showed that the highest EE (93.9%) and in vitro GI stability (8.58 log CFU/mL) was obtained with the powder encapsulated with PPI + GA. Variation in particle size was observed for all the samples. Confocal laser micrographs and vital staining revealed the highest viability of probiotic L. casei cells that were obtained with those encapsulated in PPI + GA. Thermal properties showed that the incorporation of GA increased the glass transition temperature up to 189.2°C, which represented a higher thermal stability of the powder.

Conclusions

PPI + GA coated powder was found with acceptable powder characteristics and maximum probiotic survivability.

Significance and Novelty

In this study, spray drying was used to encapsulate the probiotic bacteria which is a convenient and effective process for industrial applications. Characterization of the spray-dried encapsulated probiotic powder has been done, which helps to understand the behavior of powder in terms of solubility, flowability, thermal stability, and probiotic viability. PPI was used as carrier material, which bridges the gap between already available spray-dried products containing MD as carrier material, which could spike blood sugar levels if consumed over an extended period of time. As per the results, target product applications could include sports bars, cereals, and baking where dispersibility is not imperative.

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引用次数: 0
Impact of lipids removal on the color, cooking characteristics, textural properties, and UHPLC-QToF-IMS-based metabolites of Indian brown rice using supercritical carbon dioxide
IF 2.2 4区 农林科学 Q3 CHEMISTRY, APPLIED Pub Date : 2024-11-05 DOI: 10.1002/cche.10843
Arun Kumar, Swasti Mudgal, Ishika Jain, Narpinder Singh

Background and Objectives

The study investigated the effect of lipid extraction by using the supercritical carbon dioxide (sCO2) process from brown rice (BR) of different Indian rice varieties (PB386, PB3, PB1121, PB1637, PR122, PR123, PR129, and PR113). BR was evaluated for color, pasting, cooking, and textural properties. UHPLC-QToF-IMS analysis was also carried out to make a comparison in fatty, phenolic, and amino acid profiles between BR of different varieties.

Findings

Defatted BR (DBR) from all varieties exhibited increased L* (Lightness) while lower a* (redness to greenness) and b* (yellowness to blueness) than BR. Cooking time and hardness decreased, and water uptake increased in both types of DBR. Defatting of BR increased peak viscosity while it decreased pasting temperature in all the varieties. Fatty acids and phenolic acids compounds decreased while minor increases in amino acids were observed on defatting in all the varieties.

Conclusion

Therefore, sCO2 extraction was observed as a promising alternative to conventional methods like polishing to enhance the organoleptic and nutritional quality of the rice.

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引用次数: 0
Changes in starch properties during malting and their correlations with the malting quality of multiple barley cultivars
IF 2.2 4区 农林科学 Q3 CHEMISTRY, APPLIED Pub Date : 2024-11-05 DOI: 10.1002/cche.10846
Xiangyun Fan, Juan Zhu, Wenbin Dong, Yi Hong, Chao Lv, Peng Zhang, Rugen Xu

Background and Objective

There is still a lack of information about detailed dynamic changes in starch properties during malting among multiple barley varieties and correlations of starch properties with malting qualities. In this work, compositional, physicochemical, morphological, and structural properties of starches from four barley varieties at six time points across the malting process were first investigated to better understand the starch modification of barley malting. Furthermore, a total of 30 barley varieties were used to investigate the relationship between malting qualities and starch properties. The results provide a reference for breeding high-malting quality barley based on starch properties.

Findings

Significant decreases in starch contents, paste viscosities, relative crystallinity, as well as the ratio of 1045/1022 cm−1 of starch, were exhibited, while comparatively small changes were found in the thermal parameters during malting. Amylose and amylopectin polymers were both hydrolyzed in malting. After malting, pits and holes were discovered on the surface of some starch granules. Four parameters of peak viscosity (PV), breakdown, peak time (PeT), and pasting temperature (PT) had significant correlations with diastatic power (DP), while the above four indicators together with amylose content, total starch content, onset temperature, peak temperature, and swelling power had significant correlations with malt extract (ME).

Conclusions

The trend of changes in starch properties among all four genotypes was similar. However, the variety with better malting quality showed a greater degree of measurements in some starch characters. Starch properties are mainly related to the two malting quality indicators of DP and ME.

Significance and Novelty

PV, breakdown, PeT, and PT had significant correlations with malting qualities.

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引用次数: 0
Anti-mold properties of fermented apple juice in bread
IF 2.2 4区 农林科学 Q3 CHEMISTRY, APPLIED Pub Date : 2024-11-04 DOI: 10.1002/cche.10845
Pierre Gélinas, Nathalie Rémillard

Background and Objectives

Bread may be spoiled by mold, which results in major economic losses. The aim of this study was to optimize the anti-mold properties of apple juice fermented with Propionibacterium freudenreichii to get high levels of propionic acid with lesser amounts of acetic acid, which impairs bread taste.

Findings

After fermentation for 7 days at 35°C, apple juice (half-diluted to about 4% fermentable sugars; with 1% yeast extract; pH adjusted to 6.0) contained 1.0% propionic acid and little amounts of acetic acid (about 0.35%).

Conclusions

In bread, the anti-mold activity of fermented apple juice (5%, fb) was similar to that of apple cider vinegar (2.44%, fb).

Significance and Novelty

Fermented apple juice is a novel mold inhibitor for bread that may replace apple cider vinegar and part of calcium propionate.

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引用次数: 0
Ultrasonic-assisted enhancement of malt characteristics in amaranth: A study on physicochemical, amino acid, sugar profile, rheological properties, and muffin making
IF 2.2 4区 农林科学 Q3 CHEMISTRY, APPLIED Pub Date : 2024-10-28 DOI: 10.1002/cche.10841
Tamanna Awasthi, Narpinder Singh, Katsuyoshi Nishinari

Background and Objective

This study aimed to assess the potential of incorporating ultrasonication treatment into the soaking process before germination as a method to enhance the nutritional profile of amaranth. While ultrasound has been used to expedite germination in various grains, its impact on germinated grains from a food science perspective remains underexplored. The research focused on the effects of ultrasonication on the composition, functional, and rheological properties of amaranth grains post-germination.

Findings

The study revealed that germination alone significantly increased γ-aminobutyric acid (GABA), protein content, phenolic acids, amino acids, and total dietary fiber while reducing phytic acid and rheological parameters. Incorporating ultrasonication during soaking further elevated amino acids, GABA, protein, and phenolic acid levels. Notably, ultrasonication increased protein content by 25%, antioxidants by 35%, and dietary fiber by 20%, and attributed to cell wall breakdown and enhanced enzymatic activity during germination. Phytic acid levels decreased by up to 95% with prolonged ultrasonication, enhancing nutritional quality. Additionally, GABA levels revealed a significant rise, with an 82% and 87% increase in the Annapurna and Durga varieties, respectively. The study also found that ultrasonication led to reduced starch content, decreased pasting properties, and increased sugar content in the grains. Muffins made with ultrasonicated amaranth showed lower water activity levels, indicating better shelf stability, though specific volume decreased due to starch breakdown and increased amylase activity.

Conclusion

The study demonstrates that ultrasonication combined with germination is an effective method for enhancing the nutritional and functional properties of amaranth grains. This method significantly boosts health-promoting components like GABA, proteins, and antioxidants while also affecting key baking quality parameters.

Significance and Novelty

This research introduces a novel application of ultrasonication to improve the physiochemical, antioxidant, functional, and rheological properties of amaranth grains. The findings suggested a promising strategy for developing nutrient-rich, functional food ingredients, addressing the growing demand for healthier food products.

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引用次数: 0
Jicama (Pachyrhizus spp.), a nonconventional starch: Effects of citric acid and heat−moisture treatment on properties of starch and its application in film
IF 2.2 4区 农林科学 Q3 CHEMISTRY, APPLIED Pub Date : 2024-10-16 DOI: 10.1002/cche.10836
Anand Kishore, Anupama Singh, Kalidas Pati, Ankur Ojha

Background and Objectives

This study investigated the effects of citric acid (CA) and heat–moisture treatment (HMT) on the properties of jicama starch (JS). Furthermore, it explores the potential of modified jicama starch for film preparation.

Findings

The amylose content of the JS increases after HMT and decreases for CA-modified starch. CA and HMT modification lowered the swelling power, solubility, peak viscosity, breakdown viscosity, final viscosity, setback viscosity, and crystallinity percentage compared to the native JS. After modification, there was an increase in the transition and pasting temperature of JS. The strong peak for the control and treated JS was observed at around 1008 cm−1 wavenumber in the Fourier transform infrared spectroscopy. SEM showed the overlapping and clusters of the granules in all the modified JS. Modified JS-based film significantly affects the water solubility, barrier, and mechanical properties of film. The film's SEM showed that the CA- and HMT-modified films had more homogeneous surfaces than the native JS film.

Conclusions

HMT and CA modification significantly impacted JS properties and improved the film properties for further application.

Significance and Novelty

This study's findings will facilitate selecting an appropriate treatment to enhance the properties of JS for applications in food formulation and sustainable packaging.

背景和目的 本研究调查了柠檬酸(CA)和热湿处理(HMT)对菊芋淀粉(JS)特性的影响。此外,本研究还探讨了改性菊芋淀粉制备薄膜的潜力。 研究结果 经 HMT 处理后,JS 的直链淀粉含量增加,而 CA 改性淀粉的直链淀粉含量减少。与原生 JS 相比,CA 和 HMT 改性降低了膨胀力、溶解度、峰值粘度、分解粘度、最终粘度、后退粘度和结晶度百分比。改性后,JS 的转变温度和粘贴温度都有所提高。在傅立叶变换红外光谱中,在 1008 cm-1 波长处观察到了对照组和处理组 JS 的强峰。扫描电镜显示,所有改性聚苯乙烯中的颗粒都有重叠和团聚现象。改性聚苯乙烯薄膜会明显影响薄膜的水溶性、阻隔性和机械性能。薄膜的扫描电镜显示,CA 和 HMT 改性薄膜的表面比原生 JS 薄膜更均匀。 结论 HMT 和 CA 改性显著影响了聚苯乙烯的特性,并改善了薄膜的性能,有利于进一步应用。 意义和新颖性 本研究的发现将有助于选择适当的处理方法来增强 JS 的性能,以应用于食品配方和可持续包装。
{"title":"Jicama (Pachyrhizus spp.), a nonconventional starch: Effects of citric acid and heat−moisture treatment on properties of starch and its application in film","authors":"Anand Kishore,&nbsp;Anupama Singh,&nbsp;Kalidas Pati,&nbsp;Ankur Ojha","doi":"10.1002/cche.10836","DOIUrl":"https://doi.org/10.1002/cche.10836","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background and Objectives</h3>\u0000 \u0000 <p>This study investigated the effects of citric acid (CA) and heat–moisture treatment (HMT) on the properties of jicama starch (JS). Furthermore, it explores the potential of modified jicama starch for film preparation.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Findings</h3>\u0000 \u0000 <p>The amylose content of the JS increases after HMT and decreases for CA-modified starch. CA and HMT modification lowered the swelling power, solubility, peak viscosity, breakdown viscosity, final viscosity, setback viscosity, and crystallinity percentage compared to the native JS. After modification, there was an increase in the transition and pasting temperature of JS. The strong peak for the control and treated JS was observed at around 1008 cm<sup>−1</sup> wavenumber in the Fourier transform infrared spectroscopy. SEM showed the overlapping and clusters of the granules in all the modified JS. Modified JS-based film significantly affects the water solubility, barrier, and mechanical properties of film. The film's SEM showed that the CA- and HMT-modified films had more homogeneous surfaces than the native JS film.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>HMT and CA modification significantly impacted JS properties and improved the film properties for further application.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Significance and Novelty</h3>\u0000 \u0000 <p>This study's findings will facilitate selecting an appropriate treatment to enhance the properties of JS for applications in food formulation and sustainable packaging.</p>\u0000 </section>\u0000 </div>","PeriodicalId":9807,"journal":{"name":"Cereal Chemistry","volume":"102 2","pages":"320-331"},"PeriodicalIF":2.2,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143602826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Synthesis and characterization of hybrid gold-coated cereal particles from sorghum bran flour and wheat bran flour
IF 2.2 4区 农林科学 Q3 CHEMISTRY, APPLIED Pub Date : 2024-10-16 DOI: 10.1002/cche.10840
Amie E. Norton, Min Jung Kim, Kamaranga H. S. Peiris, Sarah Cox, Michael Tilley, Dmitriy Smolensky, Scott R. Bean

Background and Objectives

This study investigated the effect of using different phenolic-containing grain brans on nanoparticle properties.

Findings

This study successfully synthesized nanoparticles from bran flour isolated from three different sources: sorghum (Sumac and Burgundy) and hard red winter wheat (cv. 1863). The phenolic contents of the three brans were measured as 33.40, 4.98, and 1.59 mg GAE/g, respectively. NPs synthesized from the bran flour had phenolic content measuring 6.41 (sorghum-Sumac bran), 5.31 (sorghum-Burgundy bran), and 1.15 mg GAE/g (wheat bran). Adding gold to the NPs decreased phenolic content in all three cases: 2.50 (sorghum-Sumac bran), 1.80 (sorghum-Burgundy bran), and 0.30 (wheat-bran) mg GAE/g. Thus, the synthesis had an impact on the phenolic content. The sizes of the particles were measured using dynamic light scattering: 159 (sorghum-Sumac), 78 (sorghum-Burgundy), and 117 (wheat) nm, respectively. After gold was added, the sizes of the particles changed to 113 (sorghum-Sumac), 115 (sorghum-Burgundy), and 94 (wheat) nm, respectively.

Conclusions

Cereal bran is an eco-friendly source of biopolymers for producing nanoparticles. With its high phenolic content, sorghum bran is ideal for making nanoparticles with various applications. Wheat and sorghum bran is a mill by-product that can be used to synthesize nanoparticles, increasing the value of these agricultural products.

Novelty and Significance

A method was established to develop gold nanoparticles from grain bran. These methods can be used to develop value-added products across many different applications.

{"title":"Synthesis and characterization of hybrid gold-coated cereal particles from sorghum bran flour and wheat bran flour","authors":"Amie E. Norton,&nbsp;Min Jung Kim,&nbsp;Kamaranga H. S. Peiris,&nbsp;Sarah Cox,&nbsp;Michael Tilley,&nbsp;Dmitriy Smolensky,&nbsp;Scott R. Bean","doi":"10.1002/cche.10840","DOIUrl":"https://doi.org/10.1002/cche.10840","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background and Objectives</h3>\u0000 \u0000 <p>This study investigated the effect of using different phenolic-containing grain brans on nanoparticle properties.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Findings</h3>\u0000 \u0000 <p>This study successfully synthesized nanoparticles from bran flour isolated from three different sources: sorghum (Sumac and Burgundy) and hard red winter wheat (cv. 1863). The phenolic contents of the three brans were measured as 33.40, 4.98, and 1.59 mg GAE/g, respectively. NPs synthesized from the bran flour had phenolic content measuring 6.41 (sorghum-Sumac bran), 5.31 (sorghum-Burgundy bran), and 1.15 mg GAE/g (wheat bran). Adding gold to the NPs decreased phenolic content in all three cases: 2.50 (sorghum-Sumac bran), 1.80 (sorghum-Burgundy bran), and 0.30 (wheat-bran) mg GAE/g. Thus, the synthesis had an impact on the phenolic content. The sizes of the particles were measured using dynamic light scattering: 159 (sorghum-Sumac), 78 (sorghum-Burgundy), and 117 (wheat) nm, respectively. After gold was added, the sizes of the particles changed to 113 (sorghum-Sumac), 115 (sorghum-Burgundy), and 94 (wheat) nm, respectively.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Cereal bran is an eco-friendly source of biopolymers for producing nanoparticles. With its high phenolic content, sorghum bran is ideal for making nanoparticles with various applications. Wheat and sorghum bran is a mill by-product that can be used to synthesize nanoparticles, increasing the value of these agricultural products.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Novelty and Significance</h3>\u0000 \u0000 <p>A method was established to develop gold nanoparticles from grain bran. These methods can be used to develop value-added products across many different applications.</p>\u0000 </section>\u0000 </div>","PeriodicalId":9807,"journal":{"name":"Cereal Chemistry","volume":"102 1","pages":"23-33"},"PeriodicalIF":2.2,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cche.10840","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143115654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Gelation and dough-forming properties of canary seed protein concentrate in comparison with commercial soy protein concentrate and vital wheat gluten
IF 2.2 4区 农林科学 Q3 CHEMISTRY, APPLIED Pub Date : 2024-10-14 DOI: 10.1002/cche.10839
Suneru P. Perera, Connie Briggs, Pierre Hucl, Lamia L'Hocine, Michael T. Nickerson

Background and Objectives

In this study, a lab-scale process was developed to aqueously de-oil canary seed (yellow and brown-seeded) flour and derive a protein concentrate. The protein concentrates prepared were evaluated for their gelation and bread dough-forming properties.

Findings

The aqueous process successfully reduced the oil content in both yellow-seed and brown-seed roller-milled flour. The protein concentrate prepared from yellow-seed flour and brown-seed flour had 74.9% and 68.6% purity (dry weight basis), respectively. Proteins showed high resistance to thermal denaturation (peak denaturation at 107°C). It was found that the least gelation concentration of both protein concentrates was 16% (w/w). There were no significant differences in viscoelastic properties and water holding capacity between the protein gels of these two canary seed types and the addition of salt did not noticeably improve these properties. Compared to canary seed, commercial soy protein showed better gelation properties. Both yellow and brown canary seed protein showed a good potential for improving bread dough strength when incorporated into a low-gluten-strength wheat flour and was comparable to commercial vital wheat gluten at 1%–3% (w/w) inclusion levels.

Conclusions

The new canary seed protein ingredients prepared by the solvent-free aqueous-based process hold good potential for application in food formulation based on their gelation and bread dough-forming properties.

Significance and Novelty

The gelation and dough-forming properties of canary seed were not previously studied; therefore, this study provides an insight into those properties for potential food applications for canary seed proteins, which is important for canary seed market diversification as a novel protein source.

{"title":"Gelation and dough-forming properties of canary seed protein concentrate in comparison with commercial soy protein concentrate and vital wheat gluten","authors":"Suneru P. Perera,&nbsp;Connie Briggs,&nbsp;Pierre Hucl,&nbsp;Lamia L'Hocine,&nbsp;Michael T. Nickerson","doi":"10.1002/cche.10839","DOIUrl":"https://doi.org/10.1002/cche.10839","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background and Objectives</h3>\u0000 \u0000 <p>In this study, a lab-scale process was developed to aqueously de-oil canary seed (yellow and brown-seeded) flour and derive a protein concentrate. The protein concentrates prepared were evaluated for their gelation and bread dough-forming properties.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Findings</h3>\u0000 \u0000 <p>The aqueous process successfully reduced the oil content in both yellow-seed and brown-seed roller-milled flour. The protein concentrate prepared from yellow-seed flour and brown-seed flour had 74.9% and 68.6% purity (dry weight basis), respectively. Proteins showed high resistance to thermal denaturation (peak denaturation at 107°C). It was found that the least gelation concentration of both protein concentrates was 16% (w/w). There were no significant differences in viscoelastic properties and water holding capacity between the protein gels of these two canary seed types and the addition of salt did not noticeably improve these properties. Compared to canary seed, commercial soy protein showed better gelation properties. Both yellow and brown canary seed protein showed a good potential for improving bread dough strength when incorporated into a low-gluten-strength wheat flour and was comparable to commercial vital wheat gluten at 1%–3% (w/w) inclusion levels.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>The new canary seed protein ingredients prepared by the solvent-free aqueous-based process hold good potential for application in food formulation based on their gelation and bread dough-forming properties.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Significance and Novelty</h3>\u0000 \u0000 <p>The gelation and dough-forming properties of canary seed were not previously studied; therefore, this study provides an insight into those properties for potential food applications for canary seed proteins, which is important for canary seed market diversification as a novel protein source.</p>\u0000 </section>\u0000 </div>","PeriodicalId":9807,"journal":{"name":"Cereal Chemistry","volume":"102 1","pages":"11-22"},"PeriodicalIF":2.2,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143115164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Changes of gluten protein composition during sourdough fermentation in rye flour 黑麦面粉酸酵过程中面筋蛋白成分的变化
IF 2.2 4区 农林科学 Q3 CHEMISTRY, APPLIED Pub Date : 2024-10-09 DOI: 10.1002/cche.10837
Gabriella Muskovics, Alexandra Farkas, Zsuzsanna Bugyi, Sandor Tömösközi

Background and Objectives

Celiac disease (CD) is an immune-mediated condition triggered by gluten consumption. Although more studies stated that gluten proteins are partially degrading during sourdough fermentation, it is still unclear whether the CD toxic epitopes are affected. Our aim was to examine the effect of controlled sourdough fermentation on the gluten content and composition with different sourdough starters in rye flour.

Findings

Despite significant changes in protein size distribution and a decrease in secalin types confirmed by high-performance liquid chromatography methods, the enzyme-linked immunosorbent assay results showed increased gluten content after fermentation. The incomplete degradation of gluten and the potential resistance of celiac toxic epitopes may explain our results. As part of the R5 antibody binding, epitopes are supposed to be unavailable in the native form of secalins; they might be released and become accessible in the smaller protein fragments.

Conclusions

In comparison of the effect of different starter cultures, the results show that the different microbiological composition of the sourdough samples might be the reason for the differences in the protein degradation.

Significance and Novelty

Results highlight the importance of a deeper investigation of the effects of gluten protein degradation on CD toxicity and the standardization of the fermentation process.

背景与目标 乳糜泻(CD)是一种由食用麸质引发的免疫介导疾病。尽管越来越多的研究表明,面筋蛋白在酸包粉发酵过程中会部分降解,但目前仍不清楚 CD 的毒性表位是否会受到影响。我们的目的是研究控制酸包粉发酵对黑麦粉中不同酸包粉发酵剂的面筋含量和组成的影响。 研究结果 尽管经高效液相色谱法证实蛋白质大小分布发生了明显变化,仲肽酶类型减少,但酶联免疫吸附测定结果显示发酵后面筋含量增加。麸质的不完全降解和乳糜泻毒性表位的潜在抗性可能解释了我们的结果。作为 R5 抗体结合的一部分,表位在原生形式的颉颃素中应该是不可用的;它们可能会被释放出来,并在较小的蛋白质片段中变得可及。 结论 通过比较不同酵母培养物的效果,结果表明酸面团样品的微生物组成不同可能是导致蛋白质降解差异的原因。 意义和新颖性 结果凸显了深入研究面筋蛋白降解对 CD 毒性的影响以及发酵过程标准化的重要性。
{"title":"Changes of gluten protein composition during sourdough fermentation in rye flour","authors":"Gabriella Muskovics,&nbsp;Alexandra Farkas,&nbsp;Zsuzsanna Bugyi,&nbsp;Sandor Tömösközi","doi":"10.1002/cche.10837","DOIUrl":"https://doi.org/10.1002/cche.10837","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background and Objectives</h3>\u0000 \u0000 <p>Celiac disease (CD) is an immune-mediated condition triggered by gluten consumption. Although more studies stated that gluten proteins are partially degrading during sourdough fermentation, it is still unclear whether the CD toxic epitopes are affected. Our aim was to examine the effect of controlled sourdough fermentation on the gluten content and composition with different sourdough starters in rye flour.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Findings</h3>\u0000 \u0000 <p>Despite significant changes in protein size distribution and a decrease in secalin types confirmed by high-performance liquid chromatography methods, the enzyme-linked immunosorbent assay results showed increased gluten content after fermentation. The incomplete degradation of gluten and the potential resistance of celiac toxic epitopes may explain our results. As part of the R5 antibody binding, epitopes are supposed to be unavailable in the native form of secalins; they might be released and become accessible in the smaller protein fragments.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>In comparison of the effect of different starter cultures, the results show that the different microbiological composition of the sourdough samples might be the reason for the differences in the protein degradation.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Significance and Novelty</h3>\u0000 \u0000 <p>Results highlight the importance of a deeper investigation of the effects of gluten protein degradation on CD toxicity and the standardization of the fermentation process.</p>\u0000 </section>\u0000 </div>","PeriodicalId":9807,"journal":{"name":"Cereal Chemistry","volume":"101 6","pages":"1354-1363"},"PeriodicalIF":2.2,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cche.10837","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142573853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Cereal Chemistry
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