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High-Molecular-Weight Glutenin Subunit Profiles Desirable for Making Tortillas in Eastern US Soft Winter Wheat 美国东部软质冬小麦制作玉米饼所需的高分子量谷蛋白亚基谱
IF 2.2 4区 农林科学 Q3 CHEMISTRY, APPLIED Pub Date : 2025-02-26 DOI: 10.1002/cche.10876
Fengyun Ma, Byung-Kee Baik

Background and Objectives

High-molecular-weight glutenin subunits (HMW-GSs) have a major influence on gluten strength and the quality of dough-based products requiring gluten development. Eastern US soft winter (ESW) wheat with a high protein content and strong gluten strength is suitable for making tortillas; however, the role of HMW-GSs in tortilla preparation still needs to be clarified. The influence of HMW-GSs on tortilla quality was evaluated using 38 ESW wheat varieties possessing 13 predominant HMW-GS profiles.

Findings

Protein strength, as indicated by Glu-1 score, shows a negative relationship with tortilla diameter and a positive relationship with opacity and rollability scores. Subunits 7OE + 8 were associated with a small diameter and a high tortilla rollability score. ESW wheat varieties carrying subunits 5 + 10 produced tortillas with a smaller diameter and higher opacity score than those carrying subunits 2 + 12, 2 + 10 or 2 + 121.

Conclusions

ESW wheat carrying HMW-GS profile (2*, 7OE + 8, 5 + 10) or (1, 13 + 16, 5 + 10) produced tortillas of comparable quality to those prepared from commercial tortilla flours.

Significance and Novelty

This research provides ESW wheat breeders and tortilla manufacturers with an effective and efficient tool for identifying ESW wheat varieties possessing the appropriate protein characteristics for making tortillas using HMW-GS profiles and their genetic markers.

背景与目的高分子量谷蛋白亚基(HMW-GSs)对面筋强度和面筋产品质量有重要影响。美国东部软冬小麦(ESW)蛋白质含量高,面筋强度强,适合制作玉米饼;然而,HMW-GSs在玉米饼制备中的作用仍需进一步研究。以38个ESW小麦品种,13个HMW-GS优势品种为研究材料,评价了HMW-GS对玉米饼品质的影响。结果蛋白质强度与玉米饼直径呈负相关,与不透明度和可卷曲性呈正相关。亚基7OE + 8与小直径和高玉米饼可卷性评分相关。携带5 + 10亚基的ESW小麦品种比携带2 + 12、2 + 10和2 + 121亚基的品种生产的玉米饼直径更小,不透明度评分更高。结论携带HMW-GS谱(2*,7OE + 8,5 + 10)或(1,13 + 16,5 + 10)的ESW小麦制成的玉米粉质量与市售玉米粉相当。本研究为ESW小麦育种家和玉米圆饼生产商利用HMW-GS谱及其遗传标记鉴定具有适合制作玉米圆饼的蛋白质特征的ESW小麦品种提供了一种有效和高效的工具。
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引用次数: 0
Improving the Nutritional Quality of Instant Noodles Made From Wheat Flour Using Dry and Wet Fractionated Mungbean Protein Ingredients
IF 2.2 4区 农林科学 Q3 CHEMISTRY, APPLIED Pub Date : 2025-01-27 DOI: 10.1002/cche.10869
Daniel J. Skylas, Chris Whiteway, Theo Webster, Valeria Messina, Sonya Richard, Siem Doo Siah, Ken J. Quail
<div> <section> <h3> Background and Objectives</h3> <p>The nutritional quality of instant noodles can be improved using dry and wet fractionated mungbean protein ingredients. Wheat flour was blended with mungbean protein concentrate and protein isolate to produce high-protein instant noodles of around 15%, 20%, and 25% protein. The effects on nutritional composition, protein quality, texture, cooking, and color properties of instant noodles were investigated.</p> </section> <section> <h3> Findings</h3> <p>Significant differences (<i>p</i> < 0.05) were observed in the nutritional, physico-chemical, and functional properties of wheat flour and mungbean protein ingredients. Water and oil holding capacities were higher for mungbean protein isolate and protein concentrate compared to mungbean and wheat flour ingredients. Blends of wheat flour with mungbean protein concentrate and protein isolate produced instant noodles with significantly higher (<i>p</i> < 0.05) protein and mineral content, and lower fat content, compared to the wheat flour control. The protein content of instant noodles ranged from 9.1 to 23.6 g/100 g (db.). Linear regression analysis showed a strong relationship between protein content and noodle firmness (<i>R</i> = 0.965). There was a significant increase (<i>p</i> < 0.05) in lysine content (1.7–11.5 mg/g flour) with the addition of protein concentrate and isolate, improving the amino acid score of lysine from 0.34 to 0.91. The color properties of fried instant noodles showed increased redness (<i>a</i>*) and yellowness (<i>b</i>*), and lightness (<i>L</i>*) decreased with increasing protein content. There were no significant differences (<i>p</i> > 0.05) in cooking losses but there were significant differences (<i>p</i> < 0.05) in water uptake, being lower for instant noodles blended with protein isolate.</p> </section> <section> <h3> Conclusions</h3> <p>Instant noodles blended with wheat flour and mungbean protein concentrate and isolate had higher protein content, with improved nutritional composition and protein quality. Instant noodles blended with protein concentrate had significantly lower (<i>p</i> < 0.05) levels of fat and sodium content, and higher levels of calcium, magnesium, phosphorus, potassium, and zinc, compared to those blended with protein isolate. Instant noodles with improved nutritional composition have the potential to deliver increased health benefits to consumers.</p> </section> <section> <h3> Significance and Novelty</h3> <p>The key findings from this study will inform food manufacturers
背景与目的采用干湿分离的绿豆蛋白原料可以提高方便面的营养品质。将小麦粉与绿豆浓缩蛋白和分离蛋白混合,制成蛋白质含量分别为15%、20%和25%的高蛋白方便面。研究了添加剂对方便面营养成分、蛋白质品质、质地、煮熟和色泽的影响。结果小麦粉和绿豆蛋白成分在营养、理化和功能特性上存在显著差异(p < 0.05)。绿豆分离蛋白和浓缩蛋白的持水和持油能力高于绿豆和小麦粉原料。与对照组相比,小麦粉与绿豆浓缩蛋白和分离蛋白的混合物生产的方便面蛋白质和矿物质含量显著提高(p < 0.05),脂肪含量显著降低。方便面的蛋白质含量为9.1 ~ 23.6 g/100 g (db.)。线性回归分析表明,蛋白质含量与面条硬度呈极显著相关(R = 0.965)。添加精蛋白和分离蛋白显著提高了赖氨酸含量(1.7 ~ 11.5 mg/g面粉)(p < 0.05),赖氨酸氨基酸评分从0.34提高到0.91。随着蛋白质含量的增加,油炸方便面的颜色特征表现为红度(a*)和黄度(b*)增加,亮度(L*)降低。蒸煮损失量差异不显著(p > 0.05),吸水量差异显著(p < 0.05),分离蛋白方便面的吸水率较低。结论添加小麦粉和绿豆浓缩蛋白及分离蛋白的方便面蛋白质含量较高,营养成分和蛋白质品质均有改善。与分离蛋白相比,添加浓缩蛋白的方便面脂肪和钠含量显著降低(p < 0.05),钙、镁、磷、钾和锌含量较高。营养成分改善的方便面有可能为消费者带来更多的健康益处。这项研究的主要发现将告知食品制造商绿豆的健康益处,并鼓励在流行和广泛消费的食品中使用干燥的分馏绿豆蛋白浓缩成分。
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引用次数: 0
Influence of Sample Preparation Method on Total Dietary Fiber Content Measurement of Low Carbohydrate Bread Containing High Levels of Cross-Linked Phosphorylated RS4 Wheat Starch 样品制备方法对高水平交联磷酸化RS4小麦淀粉低碳水化合物面包总膳食纤维含量测定的影响
IF 2.2 4区 农林科学 Q3 CHEMISTRY, APPLIED Pub Date : 2025-01-27 DOI: 10.1002/cche.10868
Jialiang Shi, Tanya Jeradechachai, Clodualdo C. Maningat, Myeongsu Jo, Zhongwei Chen, Yong-Cheng Shi

Background and Objectives

Cross-linked phosphorylated (CLP) RS4 wheat starch has been approved as a dietary fiber source by FDA because it can reduce insulin levels following a meal containing a carbohydrate that raises blood glucose levels. It has been successfully formulated in many high-fiber and low-carbohydrate bread products. This study aimed to determine the influence of sample preparation methods on the total dietary fiber content of these fiber-fortified bread products during the assay with AOAC Method 991.43. In addition, the effects of CLP RS4 wheat starch on the morphology, crystallinity, and thermal properties of the bread were investigated.

Findings

The total dietary fiber content of breads formulated with unmodified wheat starch or CLP RS4 wheat starch was influenced by the sample preparation methods (drying, grinding, and sieving). Preferred methods are forced air oven over freeze-drier and vacuum oven; coffee grinder over mortar and pestle; and 35-mesh over 70-mesh sieve. The proposed method excludes influence of operator and shows more repeatable results. CLP RS4 wheat starch in the bread was gelatinized and had restricted swelling, reduced enthalpy, and reduced crystallinity.

Conclusions

The proposed sample preparation method generated from this study involves drying the bread in a forced air oven at 40°C, particle size reduction using a coffee grinder, and screening the ground samples in a 35-mesh sieve before total dietary fiber assay using AOAC Method 991.43.

Significance and Novelty

Our study provided new and useful information on sample preparation methodology for total dietary fiber measurement of high-fiber or low-carbohydrate bread products containing CLP RS4 wheat starch. This methodology has potential applications in many high-fiber flour-based products other than bread.

交联磷酸化(CLP) RS4小麦淀粉已被FDA批准作为膳食纤维来源,因为它可以在含有升高血糖水平的碳水化合物的膳食后降低胰岛素水平。在许多高纤维、低碳水化合物的面包产品中都成功地添加了它。在AOAC法991.43测定中,研究了不同制样方法对纤维强化面包产品总膳食纤维含量的影响。此外,还研究了CLP RS4小麦淀粉对面包的形态、结晶度和热性能的影响。结果未改性小麦淀粉和CLP RS4小麦淀粉配制的面包的总膳食纤维含量受样品制备方法(干燥、研磨和筛分)的影响。优选的方法是强制空气烘箱优于冷冻干燥器和真空烘箱;咖啡研磨机在臼和杵上;35目筛大于70目筛。该方法排除了算子的影响,结果可重复性更好。CLP RS4小麦淀粉在面包中被糊化,具有抑制膨胀、降低焓和降低结晶度的作用。本研究提出的样品制备方法是将面包在40°C的强制空气烘箱中干燥,使用咖啡研磨机减小粒度,并用35目筛筛筛选,然后使用AOAC方法991.43进行总膳食纤维测定。本研究为含CLP RS4小麦淀粉的高纤维或低碳水化合物面包制品的总膳食纤维测定提供了新的有用的样品制备方法。这种方法在除面包以外的许多高纤维面粉产品中具有潜在的应用前景。
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引用次数: 0
Phase Behavior Determines Morphology of Amylose Crystallized From Aqueous Solutions 相行为决定了直链淀粉水溶液结晶的形态
IF 2.2 4区 农林科学 Q3 CHEMISTRY, APPLIED Pub Date : 2025-01-27 DOI: 10.1002/cche.10867
Gregory R. Ziegler, John A. Creek

Background and Objectives

Here, we present a simplified system comprising amylose of varying degrees of polymerization in water, with the goal of probing the temperature–concentration phase diagram to determine if a miscibility gap leading to liquid−liquid phase separation might serve as a model for starch granule initiation and, if so, under what conditions.

Findings

A miscibility gap in the cooling-rate-dependent phase behavior is demonstrated for the amylose−water system, with its temperature and concentration depending on the degree of polymerization (DP) of the starch. Liquid–liquid phase separation within this miscibility gap followed by crystallization of the polymer-rich phase produced spherulites. If crystallization preceded liquid–liquid phase separation, a precipitate or gel was formed. The upper critical solution temperature appeared between 60°C and 70°C, and the miscibility gap was observed between 5% and 30%–50% w/w starch, depending on DP for DP ≥ 68. For DP 29, the miscibility gap occurred at concentrations ≥ 20% w/w.

Conclusions

A cooling-rate-dependent miscibility gap in the temperature–concentration phase diagram has been observed in aqueous amylose solutions under reasonable ambient conditions of temperature and starch concentration, allowing phase separation to occur within plastids in vivo.

Significance and Novelty

This work provides a biophysical complement to the biochemistry associated with starch granule initiation.

在这里,我们提出了一个由不同聚合程度的直链淀粉组成的简化体系,目的是探测温度-浓度相图,以确定导致液-液相分离的混溶间隙是否可以作为淀粉颗粒形成的模型,如果是,在什么条件下。研究结果表明直链淀粉-水体系的相行为存在一定的混相间隙,其温度和浓度取决于淀粉的聚合度。液-液相在混相间隙内分离,富聚合物相结晶生成球晶。如果结晶先于液-液相分离,则形成沉淀或凝胶。在60 ~ 70℃之间存在较高的临界溶解温度,在5% ~ 30% ~ 50% w/w淀粉之间存在混相间隙,这取决于DP≥68。DP 29在浓度≥20% w/w时出现混相间隙。结论:在合理的温度和淀粉浓度条件下,直链淀粉水溶液在温度-浓度相图中存在与冷却速率相关的混相间隙,从而使质体体内发生相分离。这项工作为淀粉颗粒起始的生物化学提供了生物物理学的补充。
{"title":"Phase Behavior Determines Morphology of Amylose Crystallized From Aqueous Solutions","authors":"Gregory R. Ziegler,&nbsp;John A. Creek","doi":"10.1002/cche.10867","DOIUrl":"https://doi.org/10.1002/cche.10867","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background and Objectives</h3>\u0000 \u0000 <p>Here, we present a simplified system comprising amylose of varying degrees of polymerization in water, with the goal of probing the temperature–concentration phase diagram to determine if a miscibility gap leading to liquid−liquid phase separation might serve as a model for starch granule initiation and, if so, under what conditions.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Findings</h3>\u0000 \u0000 <p>A miscibility gap in the cooling-rate-dependent phase behavior is demonstrated for the amylose−water system, with its temperature and concentration depending on the degree of polymerization (DP) of the starch. Liquid–liquid phase separation within this miscibility gap followed by crystallization of the polymer-rich phase produced spherulites. If crystallization preceded liquid–liquid phase separation, a precipitate or gel was formed. The upper critical solution temperature appeared between 60°C and 70°C, and the miscibility gap was observed between 5% and 30%–50% w/w starch, depending on DP for DP ≥ 68. For DP 29, the miscibility gap occurred at concentrations ≥ 20% w/w.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>A cooling-rate-dependent miscibility gap in the temperature–concentration phase diagram has been observed in aqueous amylose solutions under reasonable ambient conditions of temperature and starch concentration, allowing phase separation to occur within plastids in vivo.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Significance and Novelty</h3>\u0000 \u0000 <p>This work provides a biophysical complement to the biochemistry associated with starch granule initiation.</p>\u0000 </section>\u0000 </div>","PeriodicalId":9807,"journal":{"name":"Cereal Chemistry","volume":"102 2","pages":"386-396"},"PeriodicalIF":2.2,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143602480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Predicting Hand Folded Japanese Sponge Cake Quality Using Rapid Visco Analyzer Flour Pasting Properties 用快速粘度分析仪预测日本手折叠海绵蛋糕的质量
IF 2.2 4区 农林科学 Q3 CHEMISTRY, APPLIED Pub Date : 2025-01-27 DOI: 10.1002/cche.10870
Andrew L. Mense, Andrew S. Ross, Jayne E. Bock

Background and Objectives

The objective of this study was to evaluate a series of Rapid Visco Analyzer (RVA) methods for their ability to produce results that were predictive of hand folded Japanese sponge cake (JSC) quality. Two solvents (water and 50% [w/w] aqueous sucrose) and two temperature profiles (Standard 1 [STD 1] and an internal cake temperature profile) were selected to run across a set of flour samples from two crop years. The RVA results were previously analyzed (and published) for their ability to produce results that were predictive of mechanically folded JSC. Due to the continued use of the hand fold method, there was a need to understand how these RVA methods produced results that were predictive of hand folded JSC quality. Hand folded JSC is prepared by manually folding the flour into the egg sugar mixture using a wooden paddle, while the mechanical folding method utilizes a KitchenAid with attached paddle.

Findings

Water, 5% lactic acid, 5% sodium carbonate, and 50% sucrose SRC solvents showed no significant correlations with JSC quality. RVA viscosity results from both profiles using water were generally not significantly correlated with JSC quality while the sucrose solvent batter pasting viscosity (BPV) was significantly correlated. RVA BPV run with the internal cake temperature profile using the sucrose solvent showed significant correlations with hand folded JSC volume (r = 0.76, p ≤ 0.05), firmness (r = −0.89, p ≤ 0.01), and overall score (r = 0.79, p ≤ 0.01).

Conclusions

The RVA BPV results showed that a 50% (w/w) sucrose solvent, when compared to water, could more accurately be used to predict and screen for JSC quality outcomes. The internal cake temperature profile BPV was better correlated with hand folded JSC quality than the STD 1 profile when using sucrose solvent.

Significance and Novelty

This research identified that the RVA BPV employing the internal cake temperature profile, and a 50% sucrose solvent could estimate hand folded JSC quality using less flour and taking less time than a bake test.

背景与目的本研究的目的是评价一系列快速粘度分析仪(RVA)方法对手折叠日本海绵蛋糕(JSC)质量预测结果的能力。选择两种溶剂(水和50% [w/w]含水蔗糖)和两种温度曲线(标准1 [STD 1]和内部蛋糕温度曲线)对两个作物年的一组面粉样品进行测试。之前对RVA结果进行了分析(并发表),因为它们能够产生预测机械折叠JSC的结果。由于手折叠方法的持续使用,有必要了解这些RVA方法如何产生预测手折叠JSC质量的结果。手工折叠JSC是用木制桨将面粉手工折叠到蛋糖混合物中,而机械折叠方法则是使用带有桨的KitchenAid。水、5%乳酸、5%碳酸钠和50%蔗糖的SRC溶剂与JSC质量无显著相关性。两种方法的RVA粘度与JSC质量的相关性不显著,而蔗糖溶剂糊状粘度(BPV)与JSC质量的相关性显著。采用蔗糖溶剂对饼内温度曲线进行RVA BPV运行,与手折叠JSC体积(r = 0.76, p≤0.05)、硬度(r = - 0.89, p≤0.01)和总分(r = 0.79, p≤0.01)呈极显著相关。结论RVA BPV结果表明,与水相比,50% (w/w)的蔗糖溶剂可以更准确地预测和筛选JSC质量结果。在使用蔗糖溶剂的情况下,饼内温度曲线BPV与手折JSC品质的相关性优于STD - 1曲线。本研究发现,利用蛋糕内部温度分布和50%蔗糖溶剂的RVA BPV可以比烘焙测试使用更少的面粉和更短的时间来评估手工折叠JSC的质量。
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引用次数: 0
Evaluating the breadmaking potential of wholemeal flours from einkorn, emmer, and spelt grown in the Canadian prairies
IF 2.2 4区 农林科学 Q3 CHEMISTRY, APPLIED Pub Date : 2025-01-14 DOI: 10.1002/cche.10865
Anashwar Valsalan, Pierre Hucl, Maneka Malalgoda

Background and Objectives

Ancient grains like einkorn, emmer, and spelt remain underutilized and underexplored, limiting their market potential. This study evaluates the chemical composition, rheology, pasting, and baking properties of spring growth habit einkorn, emmer, and spelt cultivars grown in the Canadian prairies compared to wholemeal and refined hexaploid wheat.

Findings

Einkorn cultivars (CDC Aixe and CDC Marval) had inferior dough mixing properties and the lowest bread loaf volume. CDC Tatra (emmer) bread had a significantly (p < .05) higher specific volume (3.32 mL/g) than Canada Western Red Spring (CWRS) wholemeal bread.

Conclusions

Results from mixing and baking indicate that emmer and spelt cultivars have the potential to be used in breadmaking applications, while einkorn cultivars with suboptimal properties need ingredient technology and process modifications to improve their functionalities.

Significance and Novelty

The study was able to identify and characterize an emmer cultivar (CDC Tatra) with excellent mixing and baking properties having the potential as a standalone flour for baking applications. This represents a significant advancement, as prior research had not recognized any emmer cultivars for their suitability in baking. Our results highlight that cultivar-based assessment is essential in evaluating the end-use quality of ancient grain species, thereby developing products using such underutilized grains.

古老的谷物,如玉米、二粒小麦和斯佩尔特小麦,仍然没有得到充分利用和开发,限制了它们的市场潜力。本研究比较了生长在加拿大草原上的小麦品种的化学成分、流变学、糊化和烘烤特性,并将其与全麦小麦和精制六倍体小麦进行了比较。结果Einkorn品种(CDC Aixe和CDC Marval)的面团混合性能较差,面包体积最低。CDC Tatra (emmer)面包的比体积(3.32 mL/g)显著高于加拿大西部红泉(CWRS)全麦面包(p < 0.05)。结论混合和烘焙结果表明,emmer和spelt品种具有用于面包制作的潜力,而性能不理想的einkorn品种需要通过成分技术和工艺改造来提高其功能。该研究能够鉴定和表征一种具有优异混合和烘焙性能的二聚体品种(CDC Tatra),具有作为烘焙应用的独立面粉的潜力。这代表了一个重大的进步,因为以前的研究没有认识到任何二聚体栽培品种适合烘烤。我们的研究结果强调,基于品种的评估对于评估古代谷物物种的最终利用质量至关重要,从而开发利用这些未充分利用的谷物的产品。
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引用次数: 0
The effect of main component interactions on the rheological and pasting properties of naked oat flour during Sanshu thermal processing of oat-based food
IF 2.2 4区 农林科学 Q3 CHEMISTRY, APPLIED Pub Date : 2025-01-06 DOI: 10.1002/cche.10864
Pai Peng, Hamad Rafique, Xiaolong Wang, Rui Dong, Liang Zou, Xinzhong Hu

Background and Objectives

The molecular interactions in naked oat flour are crucial to its processing quality. To investigate interaction mechanisms during oat-food Sanshu thermal processing, this study analyzed starch structure properties, rheological properties, and interactions between starch and other components in naked oat flour.

Findings

The rheological and pasting properties of oat starch were closely related to its structure during different thermal stages. X-ray diffraction revealed the formation of amylose–lipid complexes, and the starch–lipid/starch–protein interactions were confirmed by specific functional groups from Fourier transform infrared spectra. The formation of complexes during roasting thermal treatment significantly increased G′ and G″, while the decomposition of starch during boiling and steaming thermal treatment reduced the rheological properties. SEM showed the dispersion and decomposition of starch, which further provided evidence for the interaction of starch and other components during Sanshu processing. The digestibility increased gradually, as the starch gelatinized during Sanshu processing. During steaming thermal treatment, RDS did not change significantly, while SDS transformed into RS (RS content increased to 9.36%).

Conclusions

The interaction of starch–lipids/starch–protein occurred during oat-food Sanshu thermal processing. The alterations in the rheological and pasting properties of naked oat flour were observed to be intimately linked to these complexes.

Significance and Novelty

The structure of naked oat flour is closely related to its processing quality. The observed changes in starch short-range order and the formation of a microcrystalline structure are closely related to RS, which can be used as a signal of interaction-resistant starch formation.

背景与目的莜麦粉中的分子相互作用对莜麦粉的加工质量至关重要。为了探讨燕麦食品三树热加工过程中的相互作用机制,本研究分析了莜麦粉中淀粉的结构特性、流变特性以及淀粉与其他组分的相互作用。发现燕麦淀粉在不同热阶段的流变性和糊化性能与其结构密切相关。x射线衍射显示了直链淀粉-脂肪复合物的形成,傅里叶变换红外光谱通过特定的官能团证实了淀粉-脂肪/淀粉-蛋白质相互作用。焙烧热处理过程中配合物的形成显著提高了G′和G″,而沸煮和蒸煮热处理过程中淀粉的分解降低了淀粉的流变性能。扫描电镜显示了淀粉的分散和分解,进一步证明了淀粉在三叔加工过程中与其他组分的相互作用。随着淀粉糊化过程的进行,消化率逐渐提高。蒸热处理过程中,RDS变化不显著,SDS转化为RS (RS含量增加至9.36%)。结论燕麦食品三枢热加工过程中存在淀粉-脂质/淀粉-蛋白相互作用。裸麦粉的流变学和糊化性质的变化与这些配合物密切相关。莜麦粉的结构与其加工质量密切相关。观察到的淀粉近程序的变化和微晶结构的形成与RS密切相关,可以作为抗相互作用淀粉形成的信号。
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引用次数: 0
Random forest regression to predict Farinograph traits from GlutoPeak output in wheat wild relative backcross lines 随机森林回归预测小麦野生相对回交系glutoppeak产量的粒度性状
IF 2.2 4区 农林科学 Q3 CHEMISTRY, APPLIED Pub Date : 2025-01-05 DOI: 10.1002/cche.10860
John H. Price, Mary J. Guttieri, Sydney Stutz

Background and Objectives

Flour quality is a key target of hard winter wheat breeding. The Farinograph is important for assessing quality before cultivar release in the United States, but large sample size requirements and long test times render it impractical for early-stage selection relative to the GlutoPeak. To improve GlutoPeak utility for breeding, we calculated new parameters from device raw output and used random forest regression to predict key Farinograph parameters in a winter wheat population containing wild relative introgressions.

Findings

The key quality parameters of absorption, bake absorption, tolerance stability, and mixing tolerance index were moderately well predicted (R2 ranging from 0.488 to 0.745). Classification of samples as acceptable or unacceptable for mixing tolerance index and tolerance stability was more accurate than prediction of numeric values.

Conclusions

New features calculated from the GlutoPeak raw data were useful predictors of quality. Prediction accuracies are sufficient to improve breeding populations.

Significance and Novelty

This study is the first to use wheat wild relative introgressions in GlutoPeak Farinograph prediction, the first to generate features from raw data, and is one of the few random forest models for quality prediction. The tools that we provide will improve ability to cull poor-quality lines early in the breeding pipeline can support efficient wheat cultivar development.

背景与目的面粉品质是硬冬小麦育种的关键指标。在美国,Farinograph对于品种发布前的质量评估是很重要的,但是相对于GlutoPeak,大样本量要求和长测试时间使得它在早期选择中不切实际。为了提高GlutoPeak对育种的效用,我们从设备的原始输出中计算新的参数,并使用随机森林回归预测含有野生相对基因渗入的冬小麦群体的关键Farinograph参数。结果吸光度、烘培吸光度、耐受性稳定性、混合耐受性指标等关键质量参数预测效果较好(R2为0.488 ~ 0.745)。混合公差指数和公差稳定性的可接受或不可接受分类比数值预测更准确。结论从GlutoPeak原始数据计算出的新特征是有效的质量预测指标。预测的准确性足以提高繁殖种群。本研究首次在GlutoPeak Farinograph预测中使用小麦野生相对渗入,首次从原始数据中生成特征,是为数不多的用于质量预测的随机森林模型之一。我们提供的工具将提高在育种管道中早期剔除劣质品系的能力,从而支持高效的小麦品种开发。
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引用次数: 0
Purple and red rice (Oryza sativa L.) bran polyphenols show antiobesity and antidiabetic activities
IF 2.2 4区 农林科学 Q3 CHEMISTRY, APPLIED Pub Date : 2024-12-31 DOI: 10.1002/cche.10861
Stephen M. Boue, William Broussard, Abigail Michelz

Background and Objectives

Rice bran is a source of bioactive polyphenols. This study aimed to characterize the antidiabetic potential of different rice brans (one brown, two red, and two purple) by examining their ability to inhibit α-amylase, α-glucosidase, and pancreatic lipase, and to stimulate glucose uptake in adipocytes.

Findings

All pigmented bran extracts significantly inhibited α-glucosidase. Both red rice brans inhibited α-amylase close to 50% at the highest dose tested. All four pigmented rice brans inhibited lipase in vitro (IC50 values 4.38–10.09 mg/mL). The pigmented rice brans studied contained higher levels of total polyphenolic content (TPC), total flavonoid content (TFC), and antioxidant capacity when compared to brown rice bran. Purple bran contained anthocyanins and red brans contained proanthocyanidins. The pigmented brans consisted of higher levels of total benzoic acids, while the brown rice bran had higher levels of total cinnamic acids. Tricin was identified in all pigmented brans.

Conclusions

Our study indicates that pigmented rice brans have the ability to manage obesity and diabetes due to their inhibitory effects on lipid and carbohydrate digestion. Several bioactive polyphenols were identified.

Significance and Novelty

The potential of pigmented rice bran to inhibit α-amylase, α-glucosidase, and lipase was confirmed.

背景与目的米糠是生物活性多酚的来源。本研究旨在通过检测不同米糠(一种棕色、两种红色和两种紫色)抑制α-淀粉酶、α-葡萄糖苷酶和胰脂肪酶的能力,以及刺激脂肪细胞葡萄糖摄取的能力,来表征不同米糠(一种棕色、两种红色和两种紫色)的抗糖尿病潜能。结果所有色素麸皮提取物均能显著抑制α-葡萄糖苷酶。在最高剂量下,两种红米糠对α-淀粉酶的抑制作用均接近50%。4种色素米糠对脂肪酶均有抑制作用(IC50值为4.38 ~ 10.09 mg/mL)。与糙米糠相比,所研究的色素米糠含有更高的总多酚含量(TPC)、总黄酮含量(TFC)和抗氧化能力。紫色麸皮含有花青素,红色麸皮含有原花青素。色素麸含有较高水平的总苯甲酸,而糙米麸含有较高水平的总肉桂酸。在所有色素脑膜中均检测到Tricin。结论:色素米糠具有抑制脂质和碳水化合物消化的作用,具有控制肥胖和糖尿病的能力。鉴定出几种具有生物活性的多酚。研究证实了色素米糠具有抑制α-淀粉酶、α-葡萄糖苷酶和脂肪酶的潜力。
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引用次数: 0
Ultrasound-assisted extraction and modification of pulse starches: A review 超声辅助提取与改性脉冲淀粉的研究进展
IF 2.2 4区 农林科学 Q3 CHEMISTRY, APPLIED Pub Date : 2024-12-31 DOI: 10.1002/cche.10862
Prudhvi Pasumarthi, Sindhu Sindhu, Annamalai Manickavasagan

Background and Objectives

Conventional pulse starch extraction methods face challenges in terms of yield, purity, and recovery. The native starches after extraction often undergo modification for broader applications. Ultrasound is considered a promising approach for starch extraction and modification due to its unique principle and reduced processing times. This work addresses the effects of ultrasound-assisted methods on extraction and characteristic modification of pulse starches.

Findings

The cavitation effect of ultrasound effectively disrupts starch–protein interactions, improves diffusion, and significantly increases the pure starch yield. When applied to starch modification, it impacts surface morphology, amylose, and amylopectin chains resulting in notable changes to the characteristic behavior. Dual modification by combining ultrasound with other methods could allow for customized starch characteristics through structural reorganization, cross-linking, and depolymerization.

Conclusions

The increased yields and modified properties of pulse starches through ultrasound-assisted methods could enable their utilization in a wide range of food and nonfood applications.

Significance and Novelty

This review provides new insights into the extraction and modification of pulse starches through ultrasound-assisted methods. It benefits researchers, food and starch industries, in selecting appropriate processing methods based on yield and specific properties of pulse starches required for their intended applications.

背景与目的传统的脉冲淀粉提取方法在得率、纯度和回收率方面面临挑战。萃取后的天然淀粉通常经过改性以获得更广泛的应用。超声波因其独特的原理和缩短加工时间而被认为是一种很有前途的淀粉提取和改性方法。本文研究了超声辅助方法对脉冲淀粉提取和特性改性的影响。发现超声空化效应有效地破坏了淀粉-蛋白相互作用,促进了扩散,显著提高了纯淀粉收率。当应用于淀粉改性时,它会影响表面形态,直链淀粉和支链淀粉链,导致特征行为发生显着变化。超声与其他方法相结合的双重改性可以通过结构重组、交联和解聚来定制淀粉的特性。结论超声辅助法制备的脉冲淀粉的产率提高,性质改变,可广泛应用于食品和非食品领域。本文综述了超声辅助提取和修饰脉冲淀粉的新方法。它有利于研究人员,食品和淀粉工业,在选择适当的加工方法的基础上,产量和特定性质的脉冲淀粉所需的预期应用。
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引用次数: 0
期刊
Cereal Chemistry
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