Ceskoslovenska farmacie最新文献

The contamination of the environment requires the content of heavy metals in medicinal plants to be limited and controlled. The present paper aims to demonstrate the possibilities of application and advantages of a modern polarographic method, differential pulse chromatography (DPP), in the identification and determination of heavy metals in the plant material. In the plant drugs Flos chamomillae and Flos calendulae, the heavy metals Pb, Cd, Ni, Zn, Cu and Fe were identified and determined. The World Health Organization (WHO) indicated Pb, Cd and Ni as dangerous toxic trace elements.

The present paper examined the effect of ionizing radiation (source, 60Co) within a range of doses from 10 to 120 kGy on amylolytic efficacy of pancreatin of two types: pancreatin obtained by isolation from an extract of the pancreas (sample 1) and pancreatin containing parts of the pancreatic tissue, but with higher amylolytic efficacy (sample 2). Efficacy was expressed in F.I.P. units. As shown in the chart, a percentual decrease in efficacy is higher in the more active sample 2. Graphical representation is in good agreement with the statistical evaluation of the significance between the decreases in efficacy in both samples irradiated with doses of 30 and 120 kGy, if the median test was used and probability was calculated with the use of Fisher's test. This is not, however, the case of irradiation with a dose of 10 kGy. The residual, graphically corrected efficacy after irradiation with a sterilizing dose of 25 kGy was 84.1% (sample 1) and 80.3% (sample 2). With the maximal dose used, the residual efficacy was 43.7% (sample 1) and 36.7% (sample 2).

Within the relationship of the structure and effect of new beta-adrenolytic agents derivatived from p-hydroxyacetophenone and p-hydroxypropiophenone with a propoxymethyl group in the lipophilic part of the molecule and with a propanamine, a butanamine and a pyrrolidine in the side-chain were studied. In order to prepare these substances, a procedure was selected from several tested ones, in which 4-hydroxy-3propoxymethylphenylketone were treated with chloromethyloxirane and subsequent reaction a hydrobromic acid were prepared 4-(3-brom-2-hydroxypropoxy)-3-propoxymethylalkylketone. Final substances were prepared reaction with amine. The structure of prepared compounds was confirmed on the basic interpretation of the IR, UV and 1H NMR spectra. The results of pharmacological evaluation of selected compounds showed a significant beta 1-blocking activity lower than acebutolol. Their local anesthetic activity is low according with their partition coefficients. The characteristic of the prepared compounds was supplemented by the determination of their partition coefficients, surface tension, dissociation constants and acute toxicity.

By pyrrolidinoethylalcohol addition on 2-, 3- and 4-alkoxy-substituted phenylisocyanate in non-aqueous toluene medium a series of 30 compounds of the type Pyrrolidinoethylesters of 2-, 3- and 4-alkoxy substituted phenylcarmabim acids were prepared. Pharmacological evaluation results shown after comparing with cocaine and procaine standards that these compounds have a higher index of effectiveness in both types of local anaesthesia. The maximum effectiveness of surface anaesthesia shows Pyrrolidinoethylester of 2-octyloxy phenylcarbamic acid which is 204-times more effective as cocaine. The maximum effectiveness of infiltration anaesthesis shows Pyrrolidinoethylester of 2-heptyloxy phenylcarbamic acid which is 66-times more effective as procaine. Prepared compounds are more effective in the case of surface anaesthesia as in the case of infiltration anaesthesia and they have comparatively high values of partition coefficients P' and P' cal. Comparison of prepared compounds effectiveness and analogic piperidinoethylesters shows that in more cases they are either equal or nearer effective. The values of their P' and P' cal correlate with these results.

Medicaments of the Kampo medicine are composed from vegetable drugs. This article describes drugs unknown in European medicine and used in Kampo preparations--Radix bupleuri, Tuber pinelliae, Hoelen, Radix scutellariae, Cortex magnoliae, Fructus zizyphi, Herba perillae, Radix paeoniae, Rhizoma atractylodis, Rhizoma alismatis, Radix alismae, Rhizoma cnidii, Radix angelicae, Rhizoma coptidis, Fructus gardeniae, Cortex phellodendri, Polyporus, Radix ginseng.

The effect of propentophylline (HWA 285) on the course of the functional regeneration of the hind extremities and on the regeneration of the energetic metabolism in the spinal cord after ischaemia produced by ligating the abdominal aorta for 20 or 30 mins was investigated. Administration of 20 mg/kg i.v. propentophylline after the induction of ischaemia produced marked regeneration of these parameters in comparison with ischaemia without administration of the drug in the course of four days. The protective effect of propentophylline administered prior to ischaemia was markedly weaker.

The present paper describes a method of dithiadenoxide determination in blood plasma. Dithiadenoxide was reduced to dithiaden with zinc dust in acidified plasma. The solution was alkalified and dithiaden extracted into n-hexane and isolated on a thin layer of silica gel using the system chloroform--n-heptane-methanol (60:22:18). After spraying the plate with a solution of m-nitrophenyldiazoniumfluoroborate in acid medium, dithiaden stains were evaluated densitometrically in the absorption maximum of the reaction product at 565 nm (RF = 0.37). Detection limit of dithiadenoxide in the proposed method is 0.004 microgram in an applied amount.