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Phytochemical Profiling and Anti-VanA Activity of Pulegone Extracted from Ziziphora tenuior Flower Against Vancomycin-Resistant Enterococci: an In Silico Approach. 从天竺葵花中提取的 Pulegone 针对耐万古霉素肠球菌的植物化学成分分析和抗 VanA 活性:一种硅学方法。
IF 2.3 3区 化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-11-04 DOI: 10.1002/cbdv.202401536
Asma Hatami, Marzieh Paeizi, Majid M M Sadeghi

Ziziphora tenuior is a herb known for its potent pharmaceutical activities. However, the specific compounds of the flowers of this herb have not been fully studied yet. This study used GC-MS to conduct a chemical analysis of the methanol and dichloromethane extracts of Z. tenuior flowers. Additionally, it sought to assess the potential antibacterial activity of the extracts against vancomycin-resistant enterococci (VRE) bacteria by predicting the interactions between one of the most prevalent compounds in the extracts and the D-alanyl-D-lactate ligase (VanA) protein, which is responsible for enterococci resistant to vancomycin. The results revealed a total of 15 compounds in the methanolic extract and 12 compounds in the dichloromethane extract. Among these, 5-methyl-2-(1-methylethylidene)-cyclohexanone, also known as pulegone, constituting 52.6 % of the methanolic extract and 34.6 % of the dichloromethane extract, was the most abundant compound in the extracts. Furthermore, the in-silico analysis demonstrated that pulegone exhibited significant interactions with VanA, as indicated by docking energy values of -7 kcal/mol and the formation of one hydrogen bond. The study suggests that pulegone shows promise as an antibacterial agent against VRE by potentially interacting with VanA protein and serving as a key inhibitor in fighting vancomycin resistance.

Ziziphora tenuior 是一种以其强大的药用活性而闻名的草药。然而,人们尚未对这种草药花的特定化合物进行充分研究。本研究使用气相色谱-质谱对 Z. tenuior 花的甲醇和二氯甲烷提取物进行了化学分析。此外,研究还试图通过预测萃取物中最常见的一种化合物与 D-丙氨酰-D-乳酸连接酶(VanA)蛋白之间的相互作用,评估萃取物对耐万古霉素肠球菌(VRE)的潜在抗菌活性。结果显示,甲醇提取物中含有 15 种化合物,二氯甲烷提取物中含有 12 种化合物。其中,5-甲基-2-(1-甲基亚乙基)-环己酮(又称 pulegone)是提取物中含量最高的化合物,占甲醇提取物的 52.6%,占二氯甲烷提取物的 34.6%。此外,室内分析表明,pulegone 与 VanA 有明显的相互作用,对接能值为 -7 kcal/mol,并形成了一个氢键。这项研究表明,pulegone 有可能与 VanA 蛋白相互作用,成为对抗万古霉素耐药性的关键抑制剂,从而有望成为一种抗 VRE 的抗菌剂。
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引用次数: 0
Linear and Polyvalent Peptides with Potent Antimicrobial Activity Against Sensitive and Multidrug-Resistant E. coli Clinical Isolates. 对敏感和耐多药大肠杆菌临床分离株具有强效抗菌活性的线性和多价肽。
IF 2.3 3区 化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-11-01 DOI: 10.1002/cbdv.202401734
Javier Eduardo García-Castañeda, Kelin Cuero-Amu, Laura Daniela Bonilla-Velásquez, Yerly Vargas-Casanova, Aura Lucía Leal-Castro, Claudia Marcela Parra-Giraldo, Amalia Giselle López-Sánchez, Ricardo Fierro-Medina, Zuly Rivera-Monroy

Peptides containing the sequences 20RRWQWR25 and 20RRWQWRMKKLG30 derived from Bovine lactoferricin (LfcinB) were synthesized and their antibacterial effect against reference strains and sensitive and resistant clinical isolates of E. coli was evaluated. Tetra-branched multiple antigen peptide (MAP) ((RRWQWR)2-K-Ahx-C)2 exhibited significant antibacterial activity against sensitive, resistant, and multidrug-resistant clinical isolates of E. coli. Peptide 3: RRWQWR-Nal-KKLG; MIC=16 µM, 26[F]: (RRWQWRFKKLG)2-K-Ahx; MIC=15 µM, 17: (RRWQWRFK)2-K-Ahx; MIC=9 µM, and LfcinB (20-25)2: (RRWQWR)2-K-Ahx; MIC=11 µM exhibited the highest antibacterial activity against E. coli strains, with bactericidal effect and haemolytic effect at MIC less than 5% and a therapeutic index >1. A synergistic effect of peptides 26[F] and 17 with ciprofloxacin (CIP) or ceftriaxone (CEF) was observed. Prolonged treatment of E. coli ATCC 25922 with sublethal concentrations of CIP induced resistance in this strain, whereas some peptides did not induce resistance. These peptides can be considered to be promising candidates for treating infections caused by resistant strains of E. coli.

合成了含有源自牛乳铁蛋白(LfcinB)的 20RRWQWR25 和 20RRWQWRMKKLG30 序列的多肽,并评估了它们对参考菌株以及敏感和耐药临床大肠杆菌分离株的抗菌效果。四分支多抗原肽(MAP)((RRWQWR)2-K-Ahx-C)2 对敏感、耐药和耐多药临床分离的大肠杆菌具有显著的抗菌活性。肽 3:RRWQWR-Nal-KKLG;MIC=16 µM,26[F]:(RRWQWRFKKLG)2-K-Ahx;MIC=15 µM,17:(RRWQWRFK)2-K-Ahx;MIC=9 µM,以及 LfcinB (20-25)2:(RRWQWR)2-K-Ahx;MIC=11 µM,对大肠杆菌菌株的抗菌活性最高。大肠杆菌菌株的抗菌活性最高,杀菌效果和溶血效果的 MIC 值小于 5%,治疗指数大于 1。多肽 26[F] 和 17 与环丙沙星(CIP)或头孢曲松(CEF)具有协同作用。用亚致死浓度的 CIP 长时间处理大肠杆菌 ATCC 25922 会诱导该菌株产生抗药性,而某些肽则不会诱导抗药性。这些多肽可被视为治疗耐药大肠杆菌菌株引起的感染的有希望的候选药物。
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引用次数: 0
Design, Synthesis, and Anti-Inflammatory Activity Evaluation of Novel Indanone Derivatives for the Treatment of Vascular Dementia. 用于治疗血管性痴呆症的新型茚满酮衍生物的设计、合成和抗炎活性评估。
IF 2.3 3区 化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-10-31 DOI: 10.1002/cbdv.202401931
Xue-Song Tang, Lin-Yu He, Sheng-Nan Li, Wen-Cheng Zhang, Ze-Yu Wu, Ai-Ling Hui

Vascular dementia (VaD) is a neurodegenerative disease resulting from cerebral vascular obstruction, leading to cognitive impairment, and currently lacks effective treatment options. Due to its complex pathogenesis, multi-target drug design (MTDLs) strategies are considered among the most promising therapeutic approaches. In this study, we designed and synthesized a series of novel indanone derivatives targeting targets related to vascular health and dementia. The results indicated that compound C5 exhibited excellent acetylcholinesterase inhibitory activity (IC50 = 1.16 ± 0.41 μM) and anti-platelet aggregation activity (IC50 = 4.92 ± 0.10 μM) within ranges of 0.1-1000 μM and 0.03-300 μM, respectively, possibly mediated by molecular docking interactions. Furthermore, compound C5 demonstrated protective effects on cells at concentrations ≤50 μM, significantly reducing the release of nitric oxide (NO), tumor necrosis factor-alpha (TNF-α), and interleukin-1 beta (IL-1β) in a concentration-dependent manner, showcasing its potent neuroinflammatory inhibitory effects. Anti-inflammatory therapies are regarded as effective strategies for treating VaD. Therefore, compound C5 holds promise as a novel candidate drug for further investigation into the treatment of vascular dementia.

血管性痴呆(VaD)是一种由脑血管阻塞导致认知障碍的神经退行性疾病,目前缺乏有效的治疗方案。由于其发病机制复杂,多靶点药物设计(MTDLs)策略被认为是最有前景的治疗方法之一。在这项研究中,我们设计并合成了一系列针对血管健康和痴呆症相关靶点的新型茚酮衍生物。结果表明,化合物 C5 表现出优异的乙酰胆碱酯酶抑制活性(IC50 = 1.16 ± 0.41 μM)和抗血小板聚集活性(IC50 = 4.92 ± 0.10 μM),范围分别为 0.1-1000 μM和 0.03-300 μM,这可能是由分子对接相互作用介导的。此外,化合物 C5 在浓度≤50 μM 时对细胞具有保护作用,以浓度依赖的方式显著减少了一氧化氮(NO)、肿瘤坏死因子-α(TNF-α)和白细胞介素-1β(IL-1β)的释放,显示了其强大的神经炎症抑制作用。抗炎疗法被认为是治疗 VaD 的有效策略。因此,化合物 C5 有希望成为治疗血管性痴呆症的新型候选药物,供进一步研究。
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引用次数: 0
Investigation into the Quantitative Structure-Biotoxicity Relationship of Antibiotics and their Estrogenic Receptor Disruption Effects. 抗生素的定量结构-生物毒性关系及其雌激素受体干扰效应研究。
IF 2.3 3区 化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-10-31 DOI: 10.1002/cbdv.202401843
Wanhong Zhu, Shuangkou Chen, Yu Wang, Xi Xu, Xia Huang, Xin Yang, Fengming Ren

In light of antibiotics being classified as environmental hormone-like compounds, their interference with the endocrine system has significantly impacted human health and ecological environments. This study employed Gaussian09 software's Density Functional Theory (DFT) to structurally optimize and perform frequency calculations on 23 representative antibiotic molecules, aiming to obtain microscopic quantum mechanical structural parameters.Physicochemical property parameters were acquired through the RDKit database in the ChemDes platform. Through multiple linear regression analysis, the primary factors affecting antibiotic biotoxicity (pLD50) were identified, leading to the establishment of a QSAR model. The predictive capability of the model was analyzed using leave-one-out cross-validation, and molecular docking was used to investigate the binding mode and mechanism of action between estrogen receptors (ER) and antibiotics. Research outcomes indicate that the established QSAR model C has regression coefficients R2 and leave-one-out cross-validation coefficients Q2 of 0.92474 and 0.74913, respectively, demonstrating good stability and predictive power. Analysis through molecular surface electrostatic potential, frontier molecular orbitals, molecular docking, and molecular dynamics revealed that the potential estrogenic disrupting effects are primarily due to hydrogen bonds and hydrophobic interactions between antibiotics and estrogen receptors. This provides a valuable exploration for identifying and screening PPCPs with potential estrogenic disrupting effects.

鉴于抗生素被归类为环境激素类化合物,其对内分泌系统的干扰严重影响着人类健康和生态环境。本研究采用高斯09软件的密度泛函理论(DFT)对23种代表性抗生素分子进行结构优化和频率计算,旨在获得微观量子力学结构参数。通过多元线性回归分析,确定了影响抗生素生物毒性(pLD50)的主要因素,从而建立了一个 QSAR 模型。利用留空交叉验证分析了该模型的预测能力,并利用分子对接研究了雌激素受体(ER)与抗生素的结合模式和作用机制。研究结果表明,建立的 QSAR 模型 C 的回归系数 R2 和一出交叉验证系数 Q2 分别为 0.92474 和 0.74913,显示出良好的稳定性和预测能力。通过分子表面静电位、前沿分子轨道、分子对接和分子动力学分析发现,潜在的雌激素干扰效应主要是由于抗生素与雌激素受体之间的氢键和疏水相互作用造成的。这为识别和筛选具有潜在雌激素干扰作用的 PPCPs 提供了有价值的探索。
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引用次数: 0
Single and Combinatorial Effects of Metformin and Thymoquinone in Diffuse Large B Cell Lymphoma Cells. 二甲双胍和胸腺醌对弥漫大 B 细胞淋巴瘤细胞的单一效应和组合效应
IF 2.3 3区 化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-10-31 DOI: 10.1002/cbdv.202401533
Una Glamočlija, Lejla Mahmutovic, Esma Bilajac, Violeta Šoljić, Katarina Vukojević, Abas Sezer, Mirza Suljagić

Diffuse large B cell lymphoma (DLBCL) is classified into Germinal Center B-cell (GCB) and activated B-cell (ABC) subgroups originating from different stages of lymphoid differentiation. Cell of origin dictates the behavior and therapeutic response of DLBCL. This study aimed to evaluate single and combinatorial effects of metformin and thymoquinone (TQ) in two DLBCL cell lines belonging to GCB and ABC subtypes. Metformin and TQ caused dose-dependent responses in both ABC and GCB DLBCL subtypes. Metformin had a greater impact on the ABC subtype while TQ demonstrated more pronounced effects on the GCB subtype. Synergistic effects were observed in the DHL4 (GCB subtype) but not in the HBL1 (ABC subtype) cell line. This is the first study to compare the effects of metformin and TQ in ABC versus GCB subtype of DLBCL. It brings valuable results that could be utilized in further research aimed at reshaping treatments for subtype-specific lymphomas.

弥漫大 B 细胞淋巴瘤(DLBCL)分为生殖中心 B 细胞(GCB)亚组和活化 B 细胞(ABC)亚组,它们起源于淋巴分化的不同阶段。起源细胞决定了 DLBCL 的行为和治疗反应。本研究旨在评估二甲双胍和胸腺醌(TQ)在两种属于GCB和ABC亚型的DLBCL细胞系中的单一作用和组合作用。二甲双胍和胸腺醌对ABC和GCB两种DLBCL亚型均产生了剂量依赖性反应。二甲双胍对ABC亚型的影响更大,而TQ对GCB亚型的影响更明显。在DHL4(GCB亚型)细胞系中观察到了协同效应,但在HBL1(ABC亚型)细胞系中没有观察到。这是第一项比较二甲双胍和TQ对ABC亚型和GCB亚型DLBCL作用的研究。它带来了有价值的结果,可用于旨在重塑亚型特异性淋巴瘤治疗方法的进一步研究。
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引用次数: 0
Beyond Conventional Drug Design: Exploring the Broad-Spectrum Efficacy of Antimicrobial Peptides. 超越传统药物设计:探索抗菌肽的广谱功效。
IF 2.3 3区 化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-10-31 DOI: 10.1002/cbdv.202401349
Tope Odunitan, Boluwatife Bridget Apanisile, Justinah Afolabi, Praise Adeniwura, Modinat Akinboade, Najahtulahi Ibrahim, Kehinde Bridget Alare, Oluwatosin Saibu, Oyindamola Adeosun, Opeyemi Hammed, Kolawole Ayiti

In the fight against pathogenic infections, antimicrobial peptides (AMPs) constitute a novel and promising class of compounds that defies accepted drug development conventions like Lipinski's rule. AMPs, often known as nature's antibiotics, are remarkably effective against a variety of pathogens, including viruses, bacteria, parasites, and fungi. Their effectiveness, despite differing from traditional drug-like properties defies accepted standards. This review investigates the complex world of AMPs with an emphasis on their structural and physicochemical properties, which include size, sequence, structure, charge, and half-life. These distinguishing characteristics set AMPs apart from medications that adhere to Lipinski's rules and greatly contribute to their selective targeting, reduction of resistance, multifunctionality, and broad-spectrum efficacy.

在抗击病原体感染的斗争中,抗菌肽(AMPs)是一类新型的、前景广阔的化合物,它打破了公认的药物开发惯例,如利平斯基规则。抗菌肽通常被称为自然界的抗生素,对病毒、细菌、寄生虫和真菌等各种病原体都有显著疗效。尽管它们与传统药物的特性不同,但其有效性却有悖于公认的标准。本综述探讨了 AMPs 的复杂世界,重点是它们的结构和理化特性,包括大小、序列、结构、电荷和半衰期。这些显著特点使 AMPs 有别于遵循李宾斯基规则的药物,并在很大程度上促进了其选择性靶向、降低抗药性、多功能性和广谱疗效。
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引用次数: 0
Plantago boissieri : Phytochemical Assessment, Antioxidant, Anti-inflammatoryand Wound Healing Potential. 车前草:植物化学评估、抗氧化、抗炎和伤口愈合潜力。
IF 2.3 3区 化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-10-31 DOI: 10.1002/cbdv.202401718
Elham Amin, Hala Abouzeid, Naglaa Afifi, Heba M Aboud, Omnia A M Abd El-Ghafar, Alya K Alabdulrahim, Hayam S Ahmed

Plantago is a large genus under family Plantaginaceae. Plantago species were noted for potent antioxidant, anti-inflammatory and wound healing activities. The current research investigated the potential bioactivities as the metabolic content of Plantagoboisserei extract. Results highlighted the rich content of phenolics (450.93 ± 7.4 mg GAE/g extract) and flavonoids (144.2 ± 3.6 mg RE/g extract). HPLC analysis enabled the detection of 17 phenolic constituents among which ellagic acid was found in highest concentration followed by rutin. P. boisserei exhibited a potent antioxidant activity evidenced by the IC50 values in ABTS and H2O2 assays (10.95 and 10.87 µg/mL, respectively) as well as in TAC assay (67.94 mg AAE/g). The anti-microbial activities revealed a moderate activity against Staphylococcus aureus and Proteus vulgaris. In vitro anti-inflammatory potential indicated a characteristic inhibition against COX-1 and COX-2 enzymes with IC50 62.8 and 14.23 µg/mL, respectively, compared to ibuprofen (IC50 8.07 and 6.58, respectively). Additionally, P. boisserei extract achieved a potent wound healing activity using in vivo rat model, this might be attributed to its high content of flavonoids together with other polyphenolic compounds that have a great free radical scavenging potential. P. boisserei is a promising candidate for more extensive phytochemical and biological exploration.

车前草是车前科的一个大属。车前草具有强大的抗氧化、消炎和伤口愈合活性。目前的研究调查了车前草提取物的潜在生物活性和代谢含量。研究结果表明,车前子提取物中含有丰富的酚类物质(450.93 ± 7.4 毫克 GAE/克提取物)和黄酮类物质(144.2 ± 3.6 毫克 RE/克提取物)。高效液相色谱分析检测出 17 种酚类成分,其中鞣花酸的浓度最高,其次是芦丁。在 ABTS 和 H2O2 试验中的 IC50 值(分别为 10.95 和 10.87 µg/mL)以及在 TAC 试验中的 IC50 值(67.94 mg AAE/g)都证明了 P. boisserei 具有很强的抗氧化活性。抗微生物活性表明,它对金黄色葡萄球菌和普通变形杆菌具有中等程度的活性。体外抗炎潜力表明,与布洛芬(IC50 分别为 8.07 和 6.58)相比,其对 COX-1 和 COX-2 酶的 IC50 分别为 62.8 和 14.23 µg/mL,具有明显的抑制作用。此外,博落回萃取物在大鼠体内模型中也具有很强的伤口愈合活性,这可能归功于它含有大量黄酮类化合物和其他多酚化合物,这些化合物具有很强的清除自由基的潜力。P. boisserei 是一种有希望进行更广泛的植物化学和生物学研究的候选植物。
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引用次数: 0
Unveiling the Substrate-Dependent Dynamics of Mycotoxin Production in Fusarium verticillioides Using an OSMAC-Metabolomics Approach. 利用 OSMAC 代谢组学方法揭示轮枝镰刀菌产生霉菌毒素的底物依赖性动态过程
IF 2.3 3区 化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-10-31 DOI: 10.1002/cbdv.202401747
Emie Groppi, Mohamed Haddad, Valérie Cristofoli, Marieke Vansteelandt, Alice Gadea

Fusarium verticillioides is a prevalent plant pathogenic fungus known to produce harmful mycotoxins, including fumonisins and emerging toxins. This study aimed to investigate the influence of substrate on the temporal patterns of mycotoxin biosynthesis by F. verticillioides, employing a combined OSMAC (One Strain-Many Compounds) strategy and metabolomics approach. The fungus was cultured under various media conditions, and samples were collected over time. LC-MS/MS analyses and a dereplicative workflow were used to profile the secondary metabolite production, focusing on mycotoxins. The results demonstrated that modifying the culture conditions led to significant variations in fungal growth and the nature and relative concentrations of mycotoxins produced. Corn meal agar (CMA) medium was favorable for fumonisins A1 and B1, while malt extract agar (MEA) favored fumonisins A2 and B2. The study also identified the production of other mycotoxins related compounds as fusarins, bikaverin derivatives and fumonisins analogs, under different growth conditions. This study highlights the potential of combining OSMAC and metabolomics to unravel the substrate-dependent and time-dependent variations in mycotoxin biosynthesis by F. verticillioides. The insights gained provide a better understanding of the ecophysiology of this fungus and the occurrence of its mycotoxins, which can inform targeted mitigation strategies to ensure food and feed safety.

轮枝镰孢菌是一种普遍存在的植物致病真菌,可产生有害的霉菌毒素,包括伏马菌毒素和新出现的毒素。本研究采用OSMAC(一种菌株-多种化合物)策略和代谢组学方法,旨在研究底物对轮纹霉菌毒素生物合成的时间模式的影响。在各种培养基条件下培养真菌,并在一段时间内采集样品。采用 LC-MS/MS 分析和去复制工作流程来分析次生代谢物的产生,重点是霉菌毒素。结果表明,改变培养条件会导致真菌生长以及所产生霉菌毒素的性质和相对浓度发生显著变化。玉米粉琼脂(CMA)培养基有利于产生伏马菌毒素 A1 和 B1,而麦芽提取物琼脂(MEA)则有利于产生伏马菌毒素 A2 和 B2。研究还发现,在不同的生长条件下,还能产生其他与霉菌毒素有关的化合物,如镰刀菌素、比卡韦林衍生物和烟曲霉毒素类似物。这项研究凸显了结合 OSMAC 和代谢组学来揭示轮枝霉菌毒素生物合成过程中底物依赖性和时间依赖性变化的潜力。所获得的洞察力有助于更好地了解这种真菌的生态生理学及其霉菌毒素的发生,从而为有针对性的缓解策略提供信息,确保食品和饲料安全。
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引用次数: 0
C-13 Norisoprenoids and Eudesmanoids from Nelumbo nucifera Gaertn. Regulate the Lipid Metabolism via the AMPK/ACC/SREBP-1c Signaling Pathway. C-13 Norisoprenoids 和 Eudesmanoids from Nelumbo nucifera Gaertn.通过 AMPK/ACC/SREBP-1c 信号通路调节脂质代谢。
IF 2.3 3区 化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-10-30 DOI: 10.1002/cbdv.202401778
Jian Jiang, Cuiling Sun, Guanghui Wang, Qinnan Xu, Yuting Bian, Jie Li, Jingdian Li, Rong Ding, Houwen Lin, Wenjing Tian, Haifeng Chen

Lotustine A (1), an undescribed C-13 norisoprenoid, along with 22 known analogues and two eudesmanoids, were isolated from the aerial parts of Nelumbo nucifera Gaertn. Among them, compounds 2, 15, 17, 21, 22, 24, 25 were isolated from N. nucifera leaves for the first time. Their structures, including absolute configurations, were elucidated by nuclear magnetic resonance, mass spectroscopy, and the modified Mosher's method. Compound 1 is the first example of C-13 norisoprenoid with a terminal double bond between C-5 and C-13. Moreover, the lipid-lowering activities of the isolates were evaluated, and the results showed that 2, 24 and 25 could remarkably decrease the levels of both total cholesterol and triglyceride in free fatty acids induced HepG2 cells at the concentration of 20 μM. The oil red staining assay further demonstrated the lipid-lowering effects of 2, 24 and 25. The western blot results indicated that compounds 2, 24 and 25 could regulate the lipid metabolism via the activation of the AMPK/ACC/SREBP-1c signaling pathway.

从 Nelumbo nucifera Gaertn 的气生部分分离出了一种未被描述的 C-13 norisoprenoid--Lotustine A(1),以及 22 种已知类似物和两种 eudesmanoids。其中,化合物 2、15、17、21、22、24 和 25 是首次从 N. nucifera 叶片中分离出来的。通过核磁共振、质谱和改进的莫舍尔法阐明了它们的结构,包括绝对构型。化合物 1 是第一个在 C-5 和 C-13 之间具有末端双键的 C-13 norisoprenoid 实例。此外,还对分离物的降脂活性进行了评估,结果表明,在 20 μM 浓度下,2、24 和 25 能显著降低游离脂肪酸诱导的 HepG2 细胞中总胆固醇和甘油三酯的水平。油红染色试验进一步证明了 2、24 和 25 的降脂作用。Western 印迹结果表明,化合物 2、24 和 25 可通过激活 AMPK/ACC/SREBP-1c 信号通路调节脂质代谢。
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引用次数: 0
Phenolic Profile of Seedless Ziziphus mauritiana Fruits and Leaves Extracts with In Vivo Antioxidant and Anti-inflammatory Activities: Influence on Pro-Inflammatory Mediators. 无籽酸枣仁果实和叶提取物的酚类概况与体内抗氧化和抗炎活性:对促炎介质的影响。
IF 2.3 3区 化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-10-30 DOI: 10.1002/cbdv.202401728
Arifa Khanam, Abdullah Ijaz Hussain, Esraa Haji Mohammed, Lutfun Nahar, Hassaan Anwer Rathore

The study aimed to assess the antioxidant and anti-inflammatory activities of polyphenol-rich extracts of seedless variety of Ziziphus mauritiana (SZM). Reverse Phase High Performance Liquid Chromatography analysis of SZM leaves and fruit extracts in ethanol revealed the presence of sixteen phenolics including chlorogenic acid, p-coumeric acid, gallic acid, kaempferol and rutin. Leaf extract showed higher total phenolic and total flavonoid contents (177.6 mg/100 g and 46.2 mg/100 g) than in fruit extract (137.8 mg/100 g and 14.1 mg/100 g). The leaf extract exhibited higher DPPH radical-scavenging activity (63.5%) than the fruit extract (58.2%). The anti-inflammatory activity was evaluated on carrageenan-induced rat model and suppression of inflammatory biomarkers (Interleukin-6, Tumor necrosis factor-α and CRP) were studied.  The fruit extract exhibited inhibition (98.1%) at the dose of 500 mg/kg body weight (BW), comparable to the indomethacin (98.4%). Both extracts suppressed the inflammatory biomarkers, but pronounced results showed by the fruit extract including CRP, IL-6, and TNF-α. The leaf extract demonstrated the higher antioxidant potential as evident from the superoxide dismutase, catalase, malondialdehyde, glutathione peroxidase and glutathione levels. These findings suggest that SZM leaf and fruit extracts possess potential antioxidant and anti-inflammatory properties and can play a significant role in mitigating oxidative stress.

该研究旨在评估无籽品种毛地黄(SZM)富含多酚的提取物的抗氧化和抗炎活性。在乙醇中反相高效液相色谱法分析了深圳柚叶和果实提取物,发现其中含有16种酚类物质,包括绿原酸、对姜黄酸、没食子酸、山柰酚和芦丁。叶提取物的总酚和总黄酮含量(177.6 毫克/100 克和 46.2 毫克/100 克)高于果提取物(137.8 毫克/100 克和 14.1 毫克/100 克)。叶提取物的 DPPH 自由基清除活性(63.5%)高于果提取物(58.2%)。对卡拉胶诱导的大鼠模型进行了抗炎活性评估,并研究了对炎症生物标志物(白细胞介素-6、肿瘤坏死因子-α和 CRP)的抑制作用。 果实提取物在剂量为 500 毫克/千克体重(BW)时的抑制率为 98.1%,与吲哚美辛(98.4%)相当。两种提取物都能抑制炎症生物标志物,但果实提取物对 CRP、IL-6 和 TNF-α 的抑制效果更明显。从超氧化物歧化酶、过氧化氢酶、丙二醛、谷胱甘肽过氧化物酶和谷胱甘肽的水平可以看出,叶提取物具有更高的抗氧化潜力。这些研究结果表明,深海鳕鱼叶和果提取物具有潜在的抗氧化和抗炎特性,可在减轻氧化应激方面发挥重要作用。
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引用次数: 0
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Chemistry & Biodiversity
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