首页 > 最新文献

Chemistry & Biodiversity最新文献

英文 中文
Phytochemical Characterization and Antibacterial Activity of Carthamus Caeruleus L. Aqueous Extracts: In Vitro and In Silico Molecular Docking Studies. Carthamus Caeruleus L. 水提取物的植物化学特征和抗菌活性:体外和硅学分子对接研究。
IF 2.3 3区 化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-11-13 DOI: 10.1002/cbdv.202402662
Yousra Belounis, Idir Moualek, Hillal Sebbane, Ali Dekir, Hamdi Bendif, Stefania Garzoli, Karim Houali

In order to valorize natural resources and the traditional use of medicinal plants in Algeria, this study exploits the antibacterial effect of Carthamus caeruleus L. Since there are few studies on this plant despite its notable therapeutic potential, this work aims to characterize the chemical composition of Carthamus caeruleus L. leaf and root aqueous extracts and to evaluate their antibacterial activity through an in vitro and in silico studies. Spectrophotometric assays and HPLC results revealed 22 components in the roots and 16 in the leaves. Disc diffusion and microdilution methods were used to study the antibacterial properties against nine standard bacterial strains. The results showed that roots exhibited the best activity on most tested strains. Both extracts were also able to inhibit the growth of Staphylococcus aureus ATCC 25923 and Escherichia coli ATSC 25922. Furthermore, no nucleic acid leakage or membrane damage was detected. However, molecular docking of the molecules indicates that some constituents have significant affinity and stability for DNA gyrase. Gallic acid, luteolin, myricetin, and orientin were found to have the highest score. The molecular docking data suggest, for the first time, that the antibacterial activity may be caused by the inhibition of DNA gyrase.

由于对这种植物的研究很少,尽管它具有显著的治疗潜力,因此本研究旨在通过体外和硅学研究,确定 Carthamus caeruleus L. 叶和根水溶液提取物的化学成分特征,并评估其抗菌活性。分光光度法和高效液相色谱法的结果显示,根中含有 22 种成分,叶中含有 16 种成分。研究人员采用了盘扩散和微量稀释法来研究其对九种标准细菌菌株的抗菌特性。结果表明,根对大多数测试菌株的活性最好。两种提取物还能抑制金黄色葡萄球菌 ATCC 25923 和大肠杆菌 ATSC 25922 的生长。此外,未检测到核酸泄漏或膜损伤。不过,分子对接表明,一些成分对 DNA 回旋酶具有显著的亲和力和稳定性。其中,没食子酸、叶黄素、杨梅素和荭草苷的得分最高。分子对接数据首次表明,抗菌活性可能是由抑制 DNA 回旋酶引起的。
{"title":"Phytochemical Characterization and Antibacterial Activity of Carthamus Caeruleus L. Aqueous Extracts: In Vitro and In Silico Molecular Docking Studies.","authors":"Yousra Belounis, Idir Moualek, Hillal Sebbane, Ali Dekir, Hamdi Bendif, Stefania Garzoli, Karim Houali","doi":"10.1002/cbdv.202402662","DOIUrl":"https://doi.org/10.1002/cbdv.202402662","url":null,"abstract":"<p><p>In order to valorize natural resources and the traditional use of medicinal plants in Algeria, this study exploits the antibacterial effect of Carthamus caeruleus L. Since there are few studies on this plant despite its notable therapeutic potential, this work aims to characterize the chemical composition of Carthamus caeruleus L. leaf and root aqueous extracts and to evaluate their antibacterial activity through an in vitro and in silico studies. Spectrophotometric assays and HPLC results revealed 22 components in the roots and 16 in the leaves. Disc diffusion and microdilution methods were used to study the antibacterial properties against nine standard bacterial strains. The results showed that roots exhibited the best activity on most tested strains. Both extracts were also able to inhibit the growth of Staphylococcus aureus ATCC 25923 and Escherichia coli ATSC 25922. Furthermore, no nucleic acid leakage or membrane damage was detected. However, molecular docking of the molecules indicates that some constituents have significant affinity and stability for DNA gyrase. Gallic acid, luteolin, myricetin, and orientin were found to have the highest score. The molecular docking data suggest, for the first time, that the antibacterial activity may be caused by the inhibition of DNA gyrase.</p>","PeriodicalId":9878,"journal":{"name":"Chemistry & Biodiversity","volume":" ","pages":"e202402662"},"PeriodicalIF":2.3,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142615813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Synthesis and Fungicidal Activity Evaluation of Novel Triazole Thione/Thioether Derivatives Containing a Pyridylpyrazole Moiety. 含吡啶吡唑分子的新型三唑硫酮/硫醚衍生物的合成与杀菌活性评估
IF 2.3 3区 化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-11-13 DOI: 10.1002/cbdv.202402388
Wenqi Fan, Xiaobing Huang, Shujing Yu, Qiang Bian, Baolei Wang

Compounds containing N-pyridylpyrazole motif have aroused interest and brought about research hotspots due to their highly-efficient insecticidal activity. The fungicidal potential of N-pyridylpyrazole derivatives has gradually been disclosed in recent years. To discover new agrochemicals with poly-heterocyclic features, a series of novel triazole thione/thioether derivatives containing pyridylpyrazole motif (8-11) was synthesized. The new compounds were identified by melting point, 1H NMR, 13C NMR, 19F NMR, HRMS, and elemental analysis. The bioassays showed that most of the pyridylpyrazole-containing triazole thione Mannich bases possessed favorable in vitro fungicidal activity toward pathogenic fungi, such as Magnaporthe oryzae, Sclerotinia sclerotiorum, Botrytis cinerea and Fusarium verticillioides, and were comparable with those of the contrast compounds A and triadimefon. Some of them exhibited moderate to good in vivo fungicidal activity against S. sclerotiorum at 0.2 mg/mL (e.g. 8f control efficacy: 60.9±3.2%). The SEM observation displayed that 8f might cause disruption of cell membrane and wall of S. sclerotiorum. Compounds 8a, 8c, 8f-8h, 8p and 9b can serve as promising new fungicidal agents to make further structural optimization. The findings in this article provide useful clue and guidance for the design and development of new poly-heterocyclic agrochemicals.

含有 N-吡啶基吡唑基团的化合物因其高效的杀虫活性而引起了人们的兴趣,并带来了研究热点。近年来,N-吡啶基吡唑衍生物的杀菌潜力也逐渐被发现。为了发现具有多杂环特征的新型农用化学品,研究人员合成了一系列含有吡啶并吡唑基团的新型三唑硫酮/硫醚衍生物(8-11)。通过熔点、1H NMR、13C NMR、19F NMR、HRMS 和元素分析对这些新化合物进行了鉴定。生物测定结果表明,大多数含吡啶并吡唑的三唑硫酮曼尼希克碱具有良好的体外杀菌活性,能有效杀灭病原真菌,如木格氏球菌(Magnaporthe oryzae)、硬粒菌(Sclerotinia sclerotiorum)、灰霉病菌(Botrytis cinerea)和疣孢镰刀菌(Fusarium verticillioides),其活性与对比化合物 A 和三唑酮相当。其中一些化合物在 0.2 毫克/毫升的浓度下对 S. sclerotiorum 具有中等至良好的体内杀菌活性(如 8f 的对照效力为 60.9±3.2%)。扫描电镜观察结果表明,8f 可能会破坏 S. sclerotiorum 的细胞膜和细胞壁。化合物 8a、8c、8f-8h、8p 和 9b 可作为有前途的新型杀菌剂,有待进一步的结构优化。本文的研究结果为设计和开发新的多杂环农用化学品提供了有用的线索和指导。
{"title":"Synthesis and Fungicidal Activity Evaluation of Novel Triazole Thione/Thioether Derivatives Containing a Pyridylpyrazole Moiety.","authors":"Wenqi Fan, Xiaobing Huang, Shujing Yu, Qiang Bian, Baolei Wang","doi":"10.1002/cbdv.202402388","DOIUrl":"https://doi.org/10.1002/cbdv.202402388","url":null,"abstract":"<p><p>Compounds containing N-pyridylpyrazole motif have aroused interest and brought about research hotspots due to their highly-efficient insecticidal activity. The fungicidal potential of N-pyridylpyrazole derivatives has gradually been disclosed in recent years. To discover new agrochemicals with poly-heterocyclic features, a series of novel triazole thione/thioether derivatives containing pyridylpyrazole motif (8-11) was synthesized. The new compounds were identified by melting point, 1H NMR, 13C NMR, 19F NMR, HRMS, and elemental analysis. The bioassays showed that most of the pyridylpyrazole-containing triazole thione Mannich bases possessed favorable in vitro fungicidal activity toward pathogenic fungi, such as Magnaporthe oryzae, Sclerotinia sclerotiorum, Botrytis cinerea and Fusarium verticillioides, and were comparable with those of the contrast compounds A and triadimefon. Some of them exhibited moderate to good in vivo fungicidal activity against S. sclerotiorum at 0.2 mg/mL (e.g. 8f control efficacy: 60.9±3.2%). The SEM observation displayed that 8f might cause disruption of cell membrane and wall of S. sclerotiorum. Compounds 8a, 8c, 8f-8h, 8p and 9b can serve as promising new fungicidal agents to make further structural optimization. The findings in this article provide useful clue and guidance for the design and development of new poly-heterocyclic agrochemicals.</p>","PeriodicalId":9878,"journal":{"name":"Chemistry & Biodiversity","volume":" ","pages":"e202402388"},"PeriodicalIF":2.3,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142615821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Triterpenoids from Potentilla chinensis inhibit RANKL-induced osteoclastogenesis in vitro and lipopolysaccharide-induced osteolytic bone loss in vivo. 五倍子中的三萜类化合物可抑制体外 RANKL 诱导的破骨细胞生成和体内脂多糖诱导的溶骨性骨质流失。
IF 2.3 3区 化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-11-13 DOI: 10.1002/cbdv.202402011
ByungSun Min, Trong Trieu Tran, Minju Gal, Manh Tuan Ha, Seungeun Hyun, Okwha Kim, Jeong Ah Kim, Jeong-Hyung Lee

In this study, a phytochemical investigation on the methanol extract of Potentilla chinensis led to the isolation of eleven triterpenoids including ursolic acid (1), pomolic acid (2), tormentic acid (3), 2-epi-corosolic acid (4), 3-epi-corosolic acid (ECA, 5), 3β-hydroxyurs-11-en-13β(28)-olide (6), euscaphic acid (7), 2-epi-tormentic acid (8), corosolic acid (9), uvaol (10), and 3-O-acetylpomolic acid (11). Among them, ECA (5) showed potential anti-osteoclastogenic activity. To the best of our knowledge, this represents the first isolation of ECA (5) from P. chinensis as well as the first investigation of its effects on osteoclast formation. Further study revealed that ECA inhibited RANKL-induced mature osteoclast formation in vitro without compromising cell viability. Mechanistically, ECA attenuated RANKL-induced mitogen-activated protein kinases (MAPKs) and nuclear factor-κB (NF-κB) activation, leading to the inhibition of c-Fos and nuclear factor of activated T cells cytoplasmic 1 (NFATc1) activation. Moreover, ECA protected against LPS-induced inflammatory bone loss and osteoclast formation in a mouse model. However, ECA did not inhibit LPS-induced inflammatory responses in macrophages. Our findings suggest that ECA mitigates LPS-induced inflammatory bone loss in mice by inhibiting RANKL-induced activation of key osteoclastogenic transcription factors, including c-Fos and NFATc1, and may be a potential natural triterpenoid for preventing or treating osteolytic diseases.

2-epi-corosolic acid (4)、3-epi-corosolic acid (ECA,5)、3β-hydroxyurs-11-en-13β(28)-olide (6)、euscaphic acid (7)、2-epi-tormentic acid (8)、corosolic acid (9)、uvaol (10) 和 3-O-acetylpomolic acid (11)。其中,ECA(5)具有潜在的抗破骨细胞生成活性。据我们所知,这是首次从盐肤木中分离出 ECA(5),也是首次研究其对破骨细胞形成的影响。进一步研究发现,ECA 可抑制 RANKL 诱导的体外成熟破骨细胞形成,同时不影响细胞活力。从机理上讲,ECA可减轻RANKL诱导的丝裂原活化蛋白激酶(MAPKs)和核因子κB(NF-κB)的活化,从而抑制c-Fos和活化T细胞胞浆核因子1(NFATc1)的活化。此外,在小鼠模型中,ECA 还能防止 LPS 诱导的炎性骨质流失和破骨细胞形成。然而,ECA 并不能抑制 LPS 诱导的巨噬细胞炎症反应。我们的研究结果表明,ECA 通过抑制 RANKL 诱导的关键破骨细胞生成转录因子(包括 c-Fos 和 NFATc1)的活化,减轻了 LPS 诱导的小鼠炎性骨质流失,可能是一种潜在的预防或治疗溶骨性疾病的天然三萜类化合物。
{"title":"Triterpenoids from Potentilla chinensis inhibit RANKL-induced osteoclastogenesis in vitro and lipopolysaccharide-induced osteolytic bone loss in vivo.","authors":"ByungSun Min, Trong Trieu Tran, Minju Gal, Manh Tuan Ha, Seungeun Hyun, Okwha Kim, Jeong Ah Kim, Jeong-Hyung Lee","doi":"10.1002/cbdv.202402011","DOIUrl":"https://doi.org/10.1002/cbdv.202402011","url":null,"abstract":"<p><p>In this study, a phytochemical investigation on the methanol extract of Potentilla chinensis led to the isolation of eleven triterpenoids including ursolic acid (1), pomolic acid (2), tormentic acid (3), 2-epi-corosolic acid (4), 3-epi-corosolic acid (ECA, 5), 3β-hydroxyurs-11-en-13β(28)-olide (6), euscaphic acid (7), 2-epi-tormentic acid (8), corosolic acid (9), uvaol (10), and 3-O-acetylpomolic acid (11). Among them, ECA (5) showed potential anti-osteoclastogenic activity. To the best of our knowledge, this represents the first isolation of ECA (5) from P. chinensis as well as the first investigation of its effects on osteoclast formation. Further study revealed that ECA inhibited RANKL-induced mature osteoclast formation in vitro without compromising cell viability. Mechanistically, ECA attenuated RANKL-induced mitogen-activated protein kinases (MAPKs) and nuclear factor-κB (NF-κB) activation, leading to the inhibition of c-Fos and nuclear factor of activated T cells cytoplasmic 1 (NFATc1) activation. Moreover, ECA protected against LPS-induced inflammatory bone loss and osteoclast formation in a mouse model. However, ECA did not inhibit LPS-induced inflammatory responses in macrophages. Our findings suggest that ECA mitigates LPS-induced inflammatory bone loss in mice by inhibiting RANKL-induced activation of key osteoclastogenic transcription factors, including c-Fos and NFATc1, and may be a potential natural triterpenoid for preventing or treating osteolytic diseases.</p>","PeriodicalId":9878,"journal":{"name":"Chemistry & Biodiversity","volume":" ","pages":"e202402011"},"PeriodicalIF":2.3,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142615761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Design, Synthesis, and Anti-Mycobacterial Evaluation of Triclosan-Isoniazid Hybrids. 三氯生-异烟肼混合物的设计、合成和抗霉菌评价
IF 2.3 3区 化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-11-13 DOI: 10.1002/cbdv.202401967
Vipan Kumar, Nikita Gupta, Shefali Chowdhary, Francoise Roquet-Baneres, Laurent Kremer

A series of Triclosan-based hybrids and their Schiff base derivatives with isoniazid were designed through in silico modelling and synthesized using copper-catalysed azide-alkyne cycloaddition (CuAAC) reaction. These compounds were then evaluated against both Mycobacterium tuberculosis (Mtb) and Mycobacterium abscessus (Mab). However, none of the synthesized hybrids exhibited significant growth inhibition, with minimum inhibitory concentration (MIC) values consistently exceeding 100 µg/mL. To further investigate these findings, we conducted mechanistic studies including thermogravimetric analysis (TGA) and UV-Vis stability tests. TGA demonstrated thermal stability of the Schiff bases up to 270 °C, while UV-Vis analysis confirmed their chemical resilience across a wide pH spectrum, showing resistance to hydrolysis under acidic and basic conditions. The lack of observed antimicrobial activity may be attributed to the high lipophilicity of these molecules, as indicated by LogP values exceeding 5, which could limit their bioavailability. These findings suggest that although combining triclosan and isoniazid holds potential, further optimization of this hybrid strategy is necessary to improve efficacy.

通过硅学建模设计了一系列基于三氯生的混合物及其与异烟肼的希夫碱衍生物,并利用铜催化叠氮-炔环加成(CuAAC)反应合成了这些化合物。然后对这些化合物进行了针对结核分枝杆菌(Mtb)和脓肿分枝杆菌(Mab)的评估。然而,合成的混合物均未表现出明显的生长抑制作用,最低抑制浓度 (MIC) 值始终超过 100 µg/mL。为了进一步研究这些发现,我们进行了机理研究,包括热重分析(TGA)和紫外可见光稳定性测试。热重分析表明希夫碱的热稳定性高达 270 °C,而紫外可见光分析则证实了它们在广泛的 pH 值范围内的化学适应性,显示出在酸性和碱性条件下的抗水解性。没有观察到抗菌活性可能是由于这些分子的亲脂性较高,LogP 值超过 5,这可能会限制它们的生物利用度。这些研究结果表明,虽然三氯生和异烟肼的组合具有潜力,但仍需进一步优化这种混合策略,以提高药效。
{"title":"Design, Synthesis, and Anti-Mycobacterial Evaluation of Triclosan-Isoniazid Hybrids.","authors":"Vipan Kumar, Nikita Gupta, Shefali Chowdhary, Francoise Roquet-Baneres, Laurent Kremer","doi":"10.1002/cbdv.202401967","DOIUrl":"https://doi.org/10.1002/cbdv.202401967","url":null,"abstract":"<p><p>A series of Triclosan-based hybrids and their Schiff base derivatives with isoniazid were designed through in silico modelling and synthesized using copper-catalysed azide-alkyne cycloaddition (CuAAC) reaction. These compounds were then evaluated against both Mycobacterium tuberculosis (Mtb) and Mycobacterium abscessus (Mab). However, none of the synthesized hybrids exhibited significant growth inhibition, with minimum inhibitory concentration (MIC) values consistently exceeding 100 µg/mL. To further investigate these findings, we conducted mechanistic studies including thermogravimetric analysis (TGA) and UV-Vis stability tests. TGA demonstrated thermal stability of the Schiff bases up to 270 °C, while UV-Vis analysis confirmed their chemical resilience across a wide pH spectrum, showing resistance to hydrolysis under acidic and basic conditions. The lack of observed antimicrobial activity may be attributed to the high lipophilicity of these molecules, as indicated by LogP values exceeding 5, which could limit their bioavailability. These findings suggest that although combining triclosan and isoniazid holds potential, further optimization of this hybrid strategy is necessary to improve efficacy.</p>","PeriodicalId":9878,"journal":{"name":"Chemistry & Biodiversity","volume":" ","pages":"e202401967"},"PeriodicalIF":2.3,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142615699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Study on Extraction and Bioactivity of Polysaccharides from Viburnum sargentii Koehne with Deep Eutectic Solvent. 用深共晶溶剂提取科恩紫檀多糖及其生物活性的研究
IF 2.3 3区 化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-11-13 DOI: 10.1002/cbdv.202402185
Lingxi Zhao, Zhuorui Zhang, Jialun Li, Hang Yu, Guiquan Jiang

The fruits of Viburnum sargentii Koehne (Vsk) are rich in bioactive polysaccharides and polyphenols, which exhibit anti-inflammatory, antioxidant, and hypoglycemic properties. This study explored the use of deep eutectic solvents (DES), specifically urea-choline chloride, as a green extraction medium to maximize polysaccharide yield from Vsk polysaccharides. Extraction conditions, including solvent composition, temperature, and time, were optimized using response surface methodology, achieving significantly higher yields and purity compared to hot water extraction. The polysaccharides extracted through DES showed a more homogeneous molecular structure, with reduced protein and pigment impurities. Bioactivity assays confirmed potent antioxidant and hypoglycemic effects, with DES-extracted polysaccharides displaying enhanced reducing power, free radical scavenging capabilities, and notable α-amylase inhibition compared to those obtained by conventional methods. These findings highlight DES extraction as an effective approach not only to improve yield and purity but also to enhance the bioactivity of extracted compounds. Consequently, DES-extracted Vsk polysaccharides show potential for use in developing functional foods and nutraceuticals. This study advances sustainable extraction technologies, positioning Vsk polysaccharides as valuable bioactive compounds with promising health applications.

Viburnum sargentii Koehne(Vsk)的果实富含生物活性多糖和多酚,具有抗炎、抗氧化和降血糖的特性。本研究探索了使用深共晶溶剂(DES),特别是尿素-氯化胆碱作为绿色萃取介质,最大限度地提高 Vsk 多糖的多糖产量。利用响应面方法对萃取条件(包括溶剂成分、温度和时间)进行了优化,与热水萃取相比,萃取率和纯度显著提高。通过 DES 提取的多糖分子结构更加均匀,蛋白质和色素杂质减少。生物活性测定证实了DES萃取多糖具有强大的抗氧化和降血糖作用,与传统方法相比,DES萃取多糖具有更强的还原力、清除自由基的能力和显著的α-淀粉酶抑制作用。这些发现突出表明,DES萃取是一种有效的方法,不仅能提高产量和纯度,还能增强萃取化合物的生物活性。因此,DES提取的Vsk多糖具有开发功能食品和营养保健品的潜力。这项研究推动了可持续萃取技术的发展,将 Vsk 多糖定位为有价值的生物活性化合物,具有广阔的健康应用前景。
{"title":"Study on Extraction and Bioactivity of Polysaccharides from Viburnum sargentii Koehne with Deep Eutectic Solvent.","authors":"Lingxi Zhao, Zhuorui Zhang, Jialun Li, Hang Yu, Guiquan Jiang","doi":"10.1002/cbdv.202402185","DOIUrl":"https://doi.org/10.1002/cbdv.202402185","url":null,"abstract":"<p><p>The fruits of Viburnum sargentii Koehne (Vsk) are rich in bioactive polysaccharides and polyphenols, which exhibit anti-inflammatory, antioxidant, and hypoglycemic properties. This study explored the use of deep eutectic solvents (DES), specifically urea-choline chloride, as a green extraction medium to maximize polysaccharide yield from Vsk polysaccharides. Extraction conditions, including solvent composition, temperature, and time, were optimized using response surface methodology, achieving significantly higher yields and purity compared to hot water extraction. The polysaccharides extracted through DES showed a more homogeneous molecular structure, with reduced protein and pigment impurities. Bioactivity assays confirmed potent antioxidant and hypoglycemic effects, with DES-extracted polysaccharides displaying enhanced reducing power, free radical scavenging capabilities, and notable α-amylase inhibition compared to those obtained by conventional methods. These findings highlight DES extraction as an effective approach not only to improve yield and purity but also to enhance the bioactivity of extracted compounds. Consequently, DES-extracted Vsk polysaccharides show potential for use in developing functional foods and nutraceuticals. This study advances sustainable extraction technologies, positioning Vsk polysaccharides as valuable bioactive compounds with promising health applications.</p>","PeriodicalId":9878,"journal":{"name":"Chemistry & Biodiversity","volume":" ","pages":"e202402185"},"PeriodicalIF":2.3,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142615817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The tannin acid /Fe3+ composite film filled with essential oil extracted from Melaleuca bracteata F. Muell leaves for the preservation of mangos. 用从 Melaleuca bracteata F. Muell 叶子中提取的精油填充的单宁酸/Fe3+ 复合薄膜,用于保存芒果。
IF 2.3 3区 化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-11-12 DOI: 10.1002/cbdv.202402221
Xiaonan Zhang, Xiaocheng Zhuang, Manxin Chen, Jingrong Wang, Zhuoyu Liu, Daxiong Qiu, Yan Huang, Weina Li, Zhiwei Liu

Tannic acid and Fe3+ were used in this study to create a polyphenol-metal network-based composite film for the preservation of Melaleuca bracteata essential oils. The inhibition rate of ABTS and DPPH free radicals reached more than 90% at an essential oil concentration of 20 mg/mL. Additive quantities of 2.0% essential oil nano-emulsion, 0.8% tannic acid/Fe3+ solution, 0.4% microcrystalline cellulose, and 1% chitosan were used to maximize the characteristics of the composite films. When combined with FTIR analysis, X-ray diffraction and scanning electron microscopy revealed that the composite film containing the essential oil emulsion had a more reticulated structure. The essential oil composite layer on mangoes increased the fruit shelf time to 12 days, decreased weight loss by 10.81±4.70%, increased the amount of soluble solids by 2.03±0.31%, and increased the amount of vitamin C by 2.18±0.09%. A trustworthy technical method for the storage and transportation of agricultural goods is offered by this study.

本研究利用单宁酸和 Fe3+ 制作了一种基于多酚-金属网络的复合薄膜,用于保存白千层精油。当精油浓度为 20 mg/mL 时,ABTS 和 DPPH 自由基的抑制率达到 90% 以上。为了最大限度地提高复合薄膜的特性,添加了 2.0% 的精油纳米乳液、0.8% 的单宁酸/Fe3+ 溶液、0.4% 的微晶纤维素和 1% 的壳聚糖。结合傅立叶变换红外分析、X 射线衍射和扫描电子显微镜,可以发现含有精油乳液的复合膜具有更多的网状结构。芒果上的精油复合膜使水果货架期延长至 12 天,重量损失减少了 10.81±4.70%,可溶性固形物含量增加了 2.03±0.31%,维生素 C 含量增加了 2.18±0.09%。这项研究为农产品的储存和运输提供了一种值得信赖的技术方法。
{"title":"The tannin acid /Fe3+ composite film filled with essential oil extracted from Melaleuca bracteata F. Muell leaves for the preservation of mangos.","authors":"Xiaonan Zhang, Xiaocheng Zhuang, Manxin Chen, Jingrong Wang, Zhuoyu Liu, Daxiong Qiu, Yan Huang, Weina Li, Zhiwei Liu","doi":"10.1002/cbdv.202402221","DOIUrl":"https://doi.org/10.1002/cbdv.202402221","url":null,"abstract":"<p><p>Tannic acid and Fe3+ were used in this study to create a polyphenol-metal network-based composite film for the preservation of Melaleuca bracteata essential oils. The inhibition rate of ABTS and DPPH free radicals reached more than 90% at an essential oil concentration of 20 mg/mL. Additive quantities of 2.0% essential oil nano-emulsion, 0.8% tannic acid/Fe3+ solution, 0.4% microcrystalline cellulose, and 1% chitosan were used to maximize the characteristics of the composite films. When combined with FTIR analysis, X-ray diffraction and scanning electron microscopy revealed that the composite film containing the essential oil emulsion had a more reticulated structure. The essential oil composite layer on mangoes increased the fruit shelf time to 12 days, decreased weight loss by 10.81±4.70%, increased the amount of soluble solids by 2.03±0.31%, and increased the amount of vitamin C by 2.18±0.09%. A trustworthy technical method for the storage and transportation of agricultural goods is offered by this study.</p>","PeriodicalId":9878,"journal":{"name":"Chemistry & Biodiversity","volume":" ","pages":"e202402221"},"PeriodicalIF":2.3,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142615828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
In silico and in vitro validation of cinnamaldehyde as a potential cathepsin B inhibitor. 肉桂醛作为潜在 cathepsin B 抑制剂的硅学和体外验证。
IF 2.3 3区 化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-11-12 DOI: 10.1002/cbdv.202401985
Chanchal Vashisth, Nitin Kumar Verma, Mozhgan Afshari, Anjaneyulu Bendi, Neera Raghav

Cancer is a leading cause of death worldwide, surpassed only by heart disease. Despite improved diagnosis and treatment, cancer cells still evade normal physiological processes such as apoptosis, metabolism, angiogenesis, cell cycle, and epigenetics. To mitigate the numerous side effects linked to chemotherapy, leveraging natural products emerged as a promising alternative, either alone or in tandem with traditional agents. Cinnamaldehyde, an active ingredient of Cinnamomum cassia's stem bark has emerged as a molecule of research with diverse pharmacological properties. In the present study, we report an in silico potential of cinnamaldehyde (CM) potential as an anticancer agent across thirteen anti-cancer targets in comparison with chlorambucil (CB), docetaxel (DOC), melphalan (MP). Computational tools such as DFT, CHEM3D, molinspiration, vNNADMET, SWISS ADME, admetSAR, galaxyrefine, iGEMDOCK, and DS-Visualizer were employed. Additionally, anti-cathepsin B activity was assessed for cinnamaldehyde and the mentioned drugs and the results showed comparable inhibition at nano Molar concentrations. The results supported molecular docking using iGEMDOCK. Both in silico and experimental findings substantiate cinnamaldehyde as a promising drug for cancer treatment including metastasis and invasion where cathepsin B involvement is indicated.

癌症是全球主要死因之一,仅次于心脏病。尽管诊断和治疗方法有所改进,但癌细胞仍会逃避正常的生理过程,如凋亡、新陈代谢、血管生成、细胞周期和表观遗传学。为了减轻化疗带来的诸多副作用,利用天然产品单独或与传统药物联用成为一种很有前景的替代方法。肉桂醛是肉桂茎皮的一种活性成分,具有多种药理特性,已成为一种研究分子。在本研究中,我们报告了肉桂醛(CM)与氯霉素(CB)、多西他赛(DOC)和美法兰(MP)相比,作为一种抗癌剂在 13 个抗癌靶点上的硅学潜力。研究采用了 DFT、CHEM3D、molinspiration、vNNADMET、SWISS ADME、admetSAR、galaxyrefine、iGEMDOCK 和 DS-Visualizer 等计算工具。此外,还对肉桂醛和上述药物的抗胰蛋白酶 B 活性进行了评估,结果表明在纳米摩尔浓度下的抑制作用相当。结果支持使用 iGEMDOCK 进行分子对接。硅学和实验结果都证明肉桂醛是一种治疗癌症(包括转移和侵袭)的有前途的药物,因为在这些癌症中都有酪蛋白酶 B 的参与。
{"title":"In silico and in vitro validation of cinnamaldehyde as a potential cathepsin B inhibitor.","authors":"Chanchal Vashisth, Nitin Kumar Verma, Mozhgan Afshari, Anjaneyulu Bendi, Neera Raghav","doi":"10.1002/cbdv.202401985","DOIUrl":"https://doi.org/10.1002/cbdv.202401985","url":null,"abstract":"<p><p>Cancer is a leading cause of death worldwide, surpassed only by heart disease. Despite improved diagnosis and treatment, cancer cells still evade normal physiological processes such as apoptosis, metabolism, angiogenesis, cell cycle, and epigenetics. To mitigate the numerous side effects linked to chemotherapy, leveraging natural products emerged as a promising alternative, either alone or in tandem with traditional agents. Cinnamaldehyde, an active ingredient of Cinnamomum cassia's stem bark has emerged as a molecule of research with diverse pharmacological properties. In the present study, we report an in silico potential of cinnamaldehyde (CM) potential as an anticancer agent across thirteen anti-cancer targets in comparison with chlorambucil (CB), docetaxel (DOC), melphalan (MP). Computational tools such as DFT, CHEM3D, molinspiration, vNNADMET, SWISS ADME, admetSAR, galaxyrefine, iGEMDOCK, and DS-Visualizer were employed. Additionally, anti-cathepsin B activity was assessed for cinnamaldehyde and the mentioned drugs and the results showed comparable inhibition at nano Molar concentrations. The results supported molecular docking using iGEMDOCK. Both in silico and experimental findings substantiate cinnamaldehyde as a promising drug for cancer treatment including metastasis and invasion where cathepsin B involvement is indicated.</p>","PeriodicalId":9878,"journal":{"name":"Chemistry & Biodiversity","volume":" ","pages":"e202401985"},"PeriodicalIF":2.3,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142615794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Invasive Candidiasis: A Promising Approach Using Jatropha Dioica Extracts and Nanotechnology. 侵袭性念珠菌病:使用麻风树提取物和纳米技术的可行方法。
IF 2.3 3区 化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-11-12 DOI: 10.1002/cbdv.202402339
Carlos Fernández-Villascan, Rosalba Patiño-Herrera, Ivonne Patino, Luis Octavio Sánchez Vargas, Daniela Salado-Leza, Elías Pérez

Candida albicans, a common fungal organism, often lives harmlessly in the human body. However, under certain conditions, it can turn into a dangerous pathogen, causing infections that range from mild to life-threatening. With rising resistance to antifungal treatments, understanding and controlling this opportunistic fungus has never been more crucial. This study highlights the potential of combining natural plant extracts, specifically the aqueous (JdextAq) and ethanolic (JdextEt) extracts of Jatropha dioica, with nanotechnology in the form of magnetite nanoparticles (MNPs) to combat this persistent pathogen. FTIR spectra revealed significant interactions between the metabolites and MNPs, specifically through binding to the Fe3+ and Fe2+ sites. The average size of the MNPs was 11±3 nm, and they are non-toxic even at high concentration (500 μg/ml). The same effect is observed with JdextEt; however, JdextAq is cytotoxic at this concentration. The JdextAq-MNPs hybrid is toxic even at very low concentrations (250-50 μg/ml). All materials demonstrated high inhibition against C. albicans. At safe concentrations for cell viability, MNPs (500 μg/ml) and JdextEt-MNPs (500-50 μg/ml) achieved the highest inhibition rates of 97.13 % and 97.56 %, respectively. As antifungal resistance rises, these findings pave the way for innovative therapeutic strategies against this opportunistic pathogen.

白色念珠菌是一种常见的真菌生物,通常在人体内无害地生存。然而,在某些条件下,它可能会变成一种危险的病原体,引起轻度到危及生命的感染。随着抗真菌治疗抗药性的增加,了解和控制这种机会性真菌变得前所未有的重要。本研究强调了将天然植物提取物(特别是麻风树的水提取物(JdextAq)和乙醇提取物(JdextEt))与磁铁矿纳米颗粒(MNPs)形式的纳米技术结合起来以对抗这种顽固病原体的潜力。傅立叶变换红外光谱显示,代谢物与 MNPs 之间存在明显的相互作用,特别是通过与 Fe3+ 和 Fe2+ 位点的结合。MNPs 的平均尺寸为 11±3 nm,即使在高浓度(500 μg/ml)下也无毒。JdextEt 也有同样的效果,但 JdextAq 在此浓度下具有细胞毒性。JdextAq-MNPs 混合物即使在极低浓度(250-50 μg/ml)下也具有毒性。所有材料都对白僵菌有很强的抑制作用。在细胞活力安全浓度下,MNPs(500 μg/ml)和 JdextEt-MNPs (500-50 μg/ml)的抑制率最高,分别达到 97.13 % 和 97.56 %。随着抗真菌耐药性的增加,这些发现为针对这种机会性病原体的创新治疗策略铺平了道路。
{"title":"Invasive Candidiasis: A Promising Approach Using Jatropha Dioica Extracts and Nanotechnology.","authors":"Carlos Fernández-Villascan, Rosalba Patiño-Herrera, Ivonne Patino, Luis Octavio Sánchez Vargas, Daniela Salado-Leza, Elías Pérez","doi":"10.1002/cbdv.202402339","DOIUrl":"https://doi.org/10.1002/cbdv.202402339","url":null,"abstract":"<p><p>Candida albicans, a common fungal organism, often lives harmlessly in the human body. However, under certain conditions, it can turn into a dangerous pathogen, causing infections that range from mild to life-threatening. With rising resistance to antifungal treatments, understanding and controlling this opportunistic fungus has never been more crucial. This study highlights the potential of combining natural plant extracts, specifically the aqueous (Jdext<sub>Aq</sub>) and ethanolic (Jdext<sub>Et</sub>) extracts of Jatropha dioica, with nanotechnology in the form of magnetite nanoparticles (MNPs) to combat this persistent pathogen. FTIR spectra revealed significant interactions between the metabolites and MNPs, specifically through binding to the Fe<sup>3+</sup> and Fe<sup>2+</sup> sites. The average size of the MNPs was 11±3 nm, and they are non-toxic even at high concentration (500 μg/ml). The same effect is observed with Jdext<sub>Et</sub>; however, Jdext<sub>Aq</sub> is cytotoxic at this concentration. The Jdext<sub>Aq</sub>-MNPs hybrid is toxic even at very low concentrations (250-50 μg/ml). All materials demonstrated high inhibition against C. albicans. At safe concentrations for cell viability, MNPs (500 μg/ml) and Jdext<sub>Et</sub>-MNPs (500-50 μg/ml) achieved the highest inhibition rates of 97.13 % and 97.56 %, respectively. As antifungal resistance rises, these findings pave the way for innovative therapeutic strategies against this opportunistic pathogen.</p>","PeriodicalId":9878,"journal":{"name":"Chemistry & Biodiversity","volume":" ","pages":"e202402339"},"PeriodicalIF":2.3,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142615801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Phenolic Components And Biological Activity Of Pomegranate. 石榴的酚类成分和生物活性。
IF 2.3 3区 化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-11-12 DOI: 10.1002/cbdv.202402301
Qin-Shi Zhao, Zhiping Zhou, Chaoyan Ma, Liyan Peng, Pengchao Hao, Sophia Yi Zhang

Pomegranate (Punica granatum L.) have been subject of extensive studies for its abundance of phytochemicals and numerous biological and medicinal properties. It is a fruit-bearing tree, which is widely consumed as a nutraceutical source as well as functional food for putative health benefits. The phenolic components are the characteristic bioactive constitutes of pomegranate, including hydrolysable tannins, flavonoids, and phenolic acids. The whole plant of this tree has many medicinal folkloric uses and good therapeutic effect, such as anticancer, antioxidant, antibacterial, antiviral, hypoglycemic, lipid-lowering, cardioprotection and digestive system protection. Through comprehensive search of available literature, this narrative review can provide an up-to-date overview of the current knowledge of characteristic bioactive constituents's structure and potential health benefits of Pomegranate, which can be used as reference for the future clinical and basic research, and also helpful for the development of pomegranate into functional food and nutraceuticals.

石榴(Punica granatum L.)因其丰富的植物化学物质和众多的生物和药用特性而成为广泛研究的对象。石榴是一种果树,作为一种营养保健品和功能性食品被广泛食用,对健康大有裨益。酚类成分是石榴特有的生物活性成分,包括可水解单宁、黄酮类化合物和酚酸。石榴全株具有多种民间药用价值和良好的治疗效果,如抗癌、抗氧化、抗菌、抗病毒、降血糖、降血脂、保护心脏和消化系统等。本综述通过对现有文献的全面检索,对目前有关石榴特征生物活性成分的结构和潜在保健作用的知识进行了最新概述,可为今后的临床和基础研究提供参考,也有助于将石榴开发成功能食品和营养保健品。
{"title":"Phenolic Components And Biological Activity Of Pomegranate.","authors":"Qin-Shi Zhao, Zhiping Zhou, Chaoyan Ma, Liyan Peng, Pengchao Hao, Sophia Yi Zhang","doi":"10.1002/cbdv.202402301","DOIUrl":"https://doi.org/10.1002/cbdv.202402301","url":null,"abstract":"<p><p>Pomegranate (Punica granatum L.) have been subject of extensive studies for its abundance of phytochemicals and numerous biological and medicinal properties. It is a fruit-bearing tree, which is widely consumed as a nutraceutical source as well as functional food for putative health benefits. The phenolic components are the characteristic bioactive constitutes of pomegranate, including hydrolysable tannins, flavonoids, and phenolic acids. The whole plant of this tree has many medicinal folkloric uses and good therapeutic effect, such as anticancer, antioxidant, antibacterial, antiviral, hypoglycemic, lipid-lowering, cardioprotection and digestive system protection. Through comprehensive search of available literature, this narrative review can provide an up-to-date overview of the current knowledge of characteristic bioactive constituents's structure and potential health benefits of Pomegranate, which can be used as reference for the future clinical and basic research, and also helpful for the development of pomegranate into functional food and nutraceuticals.</p>","PeriodicalId":9878,"journal":{"name":"Chemistry & Biodiversity","volume":" ","pages":"e202402301"},"PeriodicalIF":2.3,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142615809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Synthesis, Urease Inhibitory Activity, Molecular Docking, Dynamics, MMGBSA and DFT Studies of Schiff Bases Bearing Benzimidazole Scaffold. 含苯并咪唑支架的希夫碱的合成、尿素酶抑制活性、分子对接、动力学、MMGBSA 和 DFT 研究。
IF 2.3 3区 化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-11-12 DOI: 10.1002/cbdv.202402096
Abdul Shakoor, Faheem Jan, Sudais Rahman, Mumtaz Ali, Muhammad Ibrahim, Hammad Khan, Aftab Alam, Ajmal Khan, Abid Ali, Ebtesam Al-Olayan, Mostafa R Abukhadra, Ahmed Al-Harrasi, Momin Khan

In this study, eleven hydrazone-Schiff bases bearing benzimidazole moiety were synthesized successfully via three step reactions and structures of these products were deduced by HR-ESI-MS, 1H-, and 13C-NMR spectroscopic techniques. Lastly, these derivatives were tested for their in vitro urease inhibitory potential. Six compounds among the series attributed excellent inhibition with IC50 values of 7.20 ± 0.59 to 19.61 ± 1.10 µM better than the reference drug thiourea (IC50 = 22.12 ± 1.20 µM). Similarly, three derivatives showed significant while two compounds showed less inhibitory effects against the urease enzyme. The molecular docking analysis was carried out to reveal the binding modes and types of interaction taking place between protein (urease) and synthesized compounds. The Density Functional Theory (DFT) calculations were performed at B3LYP/6-311++G(d,p) to check the structure stability. For the account of intramolecular interaction, the DFT-D3 and Reduced Density Gradient (RDG) analysis were performed. Furthermore, the chemical nature of all compounds was explored by TD-DFT method using CAM-B3LYP functional with 6-311++G(d,p) basis set. The dynamic simulation as well as MMGBSA studies validated the binding affinity and stability of the ligand receptor complex, displaying main interactions contributing in the biological activity of the product derivatives.

本研究通过三步反应成功合成了十一种含有苯并咪唑分子的腙-希夫碱,并利用 HR-ESI-MS、1H- 和 13C-NMR 光谱技术推断了这些产物的结构。最后,对这些衍生物进行了体外脲酶抑制潜力测试。该系列中的六个化合物具有出色的抑制作用,其 IC50 值为 7.20 ± 0.59 至 19.61 ± 1.10 µM,优于参考药物硫脲(IC50 = 22.12 ± 1.20 µM)。同样,三种衍生物对脲酶的抑制作用明显,而两种化合物的抑制作用较弱。为了揭示蛋白质(尿素酶)与合成化合物之间的结合模式和相互作用类型,我们进行了分子对接分析。在 B3LYP/6-311++G(d,p) 下进行了密度泛函理论(DFT)计算,以检验结构的稳定性。为了考虑分子内相互作用,还进行了 DFT-D3 和还原密度梯度(RDG)分析。此外,还使用 6-311++G(d,p) 基集的 CAM-B3LYP 函数,通过 TD-DFT 方法探讨了所有化合物的化学性质。动态模拟和 MMGBSA 研究验证了配体受体复合物的结合亲和力和稳定性,显示了有助于提高产品衍生物生物活性的主要相互作用。
{"title":"Synthesis, Urease Inhibitory Activity, Molecular Docking, Dynamics, MMGBSA and DFT Studies of Schiff Bases Bearing Benzimidazole Scaffold.","authors":"Abdul Shakoor, Faheem Jan, Sudais Rahman, Mumtaz Ali, Muhammad Ibrahim, Hammad Khan, Aftab Alam, Ajmal Khan, Abid Ali, Ebtesam Al-Olayan, Mostafa R Abukhadra, Ahmed Al-Harrasi, Momin Khan","doi":"10.1002/cbdv.202402096","DOIUrl":"https://doi.org/10.1002/cbdv.202402096","url":null,"abstract":"<p><p>In this study, eleven hydrazone-Schiff bases bearing benzimidazole moiety were synthesized successfully via three step reactions and structures of these products were deduced by HR-ESI-MS, 1H-, and 13C-NMR spectroscopic techniques. Lastly, these derivatives were tested for their in vitro urease inhibitory potential. Six compounds among the series attributed excellent inhibition with IC50 values of 7.20 ± 0.59 to 19.61 ± 1.10 µM better than the reference drug thiourea (IC50 = 22.12 ± 1.20 µM). Similarly, three derivatives showed significant while two compounds showed less inhibitory effects against the urease enzyme. The molecular docking analysis was carried out to reveal the binding modes and types of interaction taking place between protein (urease) and synthesized compounds. The Density Functional Theory (DFT) calculations were performed at B3LYP/6-311++G(d,p) to check the structure stability. For the account of intramolecular interaction, the DFT-D3 and Reduced Density Gradient (RDG) analysis were performed. Furthermore, the chemical nature of all compounds was explored by TD-DFT method using CAM-B3LYP functional with 6-311++G(d,p) basis set. The dynamic simulation as well as MMGBSA studies validated the binding affinity and stability of the ligand receptor complex, displaying main interactions contributing in the biological activity of the product derivatives.</p>","PeriodicalId":9878,"journal":{"name":"Chemistry & Biodiversity","volume":" ","pages":"e202402096"},"PeriodicalIF":2.3,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142615824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Chemistry & Biodiversity
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1