Inasse Cherfi, Nasma Mahboub, Ikram Toumi, Salah Eddine Laouini, Gamil Gamal Hasan, Abderrhmane Bouafia, Fahad Alharthi, Talha Bin Emran
Polycystic Ovarian Syndrome (PCOS) is characterized by metabolic and reproductive dysfunction, often associated with elevated oxidative stress markers in the bloodstream. This study examines the potential antioxidant properties and α-amylase inhibitory activity of Artemisia campestris leaves extract (Artemisia campestris L) and its effects on rats with induced PCOS. Estradiol valerate was administered to ten mature Wistar rats to induce PCOS, while a control group consisted of five mature Wistar rats. Following a 16-day induction period, the rats were categorized into three groups: a control group, a PCOS group, and an experimental group receiving 200 mg/kg body weight of A. campestris L. extract orally for 15 days. Serum levels of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) were measured using the ELISA technique. The group treated with A. campestris L. extract exhibited significantly reduced LH levels compared to the PCOS group. Histomorphometric analysis indicated notable changes in follicle counts and the thickness of the theca layer. These findings suggest a significant alleviation of PCOS symptoms, potentially linked to the effects of A. campestris L. on oxidative stress pathways. Furthermore, aqueous extracts of A. campestris L. displayed potent in vitro inhibition of α-amylase, with an IC50 value of 2.418 µg/mL.
多囊卵巢综合征(PCOS)以代谢和生殖功能障碍为特征,通常与血液中氧化应激指标升高有关。本研究探讨了青蒿叶提取物(青蒿 L)的潜在抗氧化特性、α-淀粉酶抑制活性及其对诱发多囊卵巢综合征大鼠的影响。对 10 只成熟的 Wistar 大鼠施用戊酸雌二醇诱导多囊卵巢综合征,对照组由 5 只成熟的 Wistar 大鼠组成。经过 16 天的诱导期后,大鼠被分为三组:对照组、多囊卵巢综合征组和连续 15 天口服 200 毫克/公斤体重的 A. campestris L. 提取物的实验组。采用 ELISA 技术测定血清中的卵泡刺激素(FSH)和黄体生成素(LH)水平。与多囊卵巢综合征组相比,接受 A. campestris L. 提取物治疗的组的 LH 水平明显降低。组织形态分析表明,卵泡数量和子宫颈层厚度发生了明显变化。这些研究结果表明,多囊卵巢综合症的症状明显减轻,这可能与 A. campestris L. 对氧化应激途径的影响有关。此外,A. campestris L.的水提取物对α-淀粉酶有很强的体外抑制作用,其 IC50 值为 2.418 µg/mL。
{"title":"Assessment of Artemisia campestris L. Leaf Extract Effects on Polycystic Ovarian Syndrome in Rats, Antioxidant and α-Amylase Inhibition Activities.","authors":"Inasse Cherfi, Nasma Mahboub, Ikram Toumi, Salah Eddine Laouini, Gamil Gamal Hasan, Abderrhmane Bouafia, Fahad Alharthi, Talha Bin Emran","doi":"10.1002/cbdv.202402184","DOIUrl":"https://doi.org/10.1002/cbdv.202402184","url":null,"abstract":"<p><p>Polycystic Ovarian Syndrome (PCOS) is characterized by metabolic and reproductive dysfunction, often associated with elevated oxidative stress markers in the bloodstream. This study examines the potential antioxidant properties and α-amylase inhibitory activity of Artemisia campestris leaves extract (Artemisia campestris L) and its effects on rats with induced PCOS. Estradiol valerate was administered to ten mature Wistar rats to induce PCOS, while a control group consisted of five mature Wistar rats. Following a 16-day induction period, the rats were categorized into three groups: a control group, a PCOS group, and an experimental group receiving 200 mg/kg body weight of A. campestris L. extract orally for 15 days. Serum levels of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) were measured using the ELISA technique. The group treated with A. campestris L. extract exhibited significantly reduced LH levels compared to the PCOS group. Histomorphometric analysis indicated notable changes in follicle counts and the thickness of the theca layer. These findings suggest a significant alleviation of PCOS symptoms, potentially linked to the effects of A. campestris L. on oxidative stress pathways. Furthermore, aqueous extracts of A. campestris L. displayed potent in vitro inhibition of α-amylase, with an IC50 value of 2.418 µg/mL.</p>","PeriodicalId":9878,"journal":{"name":"Chemistry & Biodiversity","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142496049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A series of innovative benzo[4,5]imidazo[1,2-b]pyrazole scaffold containing compounds were rationally designed through a ring-closure scaffold hopping strategy and synthetized with an intermediate derivatization approach. Physicochemical properties analysis indicated the potential pesticide-likeness of the target compounds. The optimal target compound A14 showed relatively good insecticidal activity against P. xylostella, with an LC50 value of 37.58 mg/L, and demonstrated lower acute fish toxicity compared to fipronil. Docking binding mode analysis demonstrated that compound A14 bound to GABAR through a H-bond between the amide group and the residue of 6'Thr. The differences in binding modes between benzo[4,5]imidazo[1,2-b]pyrazole target compounds and fipronil may be a key factor for the reduced insecticidal activities. The elucidated binding mode and SAR profile lay a foundation for the further structural optimization of insecticidal benzo[4,5]imidazo[1,2-b]pyrazole derivatives.
通过环闭支架跳转策略合理设计了一系列含有苯并[4,5]咪唑并[1,2-b]吡唑支架的创新化合物,并采用中间体衍生化方法合成了这些化合物。理化性质分析表明,目标化合物具有潜在的农药相似性。最佳目标化合物 A14 对木虱具有较好的杀虫活性,半数致死浓度为 37.58 毫克/升,与氟虫腈相比,其对鱼类的急性毒性更低。Docking 结合模式分析表明,化合物 A14 通过酰胺基团与 6'Thr 残基之间的 H 键与 GABAR 结合。苯并[4,5]咪唑并[1,2-b]吡唑目标化合物与氟虫腈结合模式的差异可能是导致杀虫活性降低的关键因素。阐明的结合模式和 SAR 特征为进一步优化杀虫苯并[4,5]咪唑并[1,2-b]吡唑衍生物的结构奠定了基础。
{"title":"Novel Insecticidal Benzo[4,5]imidazo[1,2-b]pyrazole Derivatives Identified through Ring-Closure Scaffold Hopping on Fipronil.","authors":"Cong Zhou, Guanglong Li, Sihui Wang, Zhong Li, Xuhong Qian, Jiagao Cheng","doi":"10.1002/cbdv.202402148","DOIUrl":"https://doi.org/10.1002/cbdv.202402148","url":null,"abstract":"<p><p>A series of innovative benzo[4,5]imidazo[1,2-b]pyrazole scaffold containing compounds were rationally designed through a ring-closure scaffold hopping strategy and synthetized with an intermediate derivatization approach. Physicochemical properties analysis indicated the potential pesticide-likeness of the target compounds. The optimal target compound A14 showed relatively good insecticidal activity against P. xylostella, with an LC50 value of 37.58 mg/L, and demonstrated lower acute fish toxicity compared to fipronil. Docking binding mode analysis demonstrated that compound A14 bound to GABAR through a H-bond between the amide group and the residue of 6'Thr. The differences in binding modes between benzo[4,5]imidazo[1,2-b]pyrazole target compounds and fipronil may be a key factor for the reduced insecticidal activities. The elucidated binding mode and SAR profile lay a foundation for the further structural optimization of insecticidal benzo[4,5]imidazo[1,2-b]pyrazole derivatives.</p>","PeriodicalId":9878,"journal":{"name":"Chemistry & Biodiversity","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142496059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Doaa Ahmed Korany, Nilofar Nilofar, Gokhan Zengin, Omayma A Eldahshan
Platycladus orientalis leaves are widely used in traditional medicine to treat different ailments. In the present study, the volatile constituents were obtained by n-hexane extraction and hydrodistillation. Comprehensive metabolomic profiling was performed using GC-MS analysis. Furthermore, in vitro antioxidant potential and enzyme-inhibitory activity were assessed and supported by in silico profiling. Results revealed the predominance of monoterpene hydrocarbons in the hydrodistilled volatile oil (42.30%) followed by oxygenated sesquiterpenes (32.10%); with cedrol as the main component. Diterpenoids (49.70%) and sesquiterpene hydrocarbons (13.43%) were the major components of the n-hexane extract; with vulgarol A, a diterpene alcohol, as the major constituent. The volatile oil demonstrated significantly higher antioxidant potential across all assays, including ABTS and DDPH scavenging activity, CUPRAC, and FRAP assays. However, the n-hexane extract demonstrated broad inhibitory effects against butyrylcholinesterase, tyrosinase, α-amylase, and α-glucosidase enzymes, supported by molecular docking study and predictive ADME profiling. Therefore, it may be concluded that the n-hexane extract is a viable option for treating dysregulated enzyme conditions. In addition, the potential use of volatile oil in the pharmaceutical industries and management of oxidative stress can be inferred. These results warrant further studies to validate the therapeutic potential of the volatile oil and the n-hexane extract.
{"title":"Chemical Constituents, Antioxidant, and Enzyme Inhibitory Potentials Supported by In-Silico Studies of the n-Hexane Extract and Essential Oil of Platycladus orientalis (L.) Franco Leaves.","authors":"Doaa Ahmed Korany, Nilofar Nilofar, Gokhan Zengin, Omayma A Eldahshan","doi":"10.1002/cbdv.202402000","DOIUrl":"https://doi.org/10.1002/cbdv.202402000","url":null,"abstract":"<p><p>Platycladus orientalis leaves are widely used in traditional medicine to treat different ailments. In the present study, the volatile constituents were obtained by n-hexane extraction and hydrodistillation. Comprehensive metabolomic profiling was performed using GC-MS analysis. Furthermore, in vitro antioxidant potential and enzyme-inhibitory activity were assessed and supported by in silico profiling. Results revealed the predominance of monoterpene hydrocarbons in the hydrodistilled volatile oil (42.30%) followed by oxygenated sesquiterpenes (32.10%); with cedrol as the main component. Diterpenoids (49.70%) and sesquiterpene hydrocarbons (13.43%) were the major components of the n-hexane extract; with vulgarol A, a diterpene alcohol, as the major constituent. The volatile oil demonstrated significantly higher antioxidant potential across all assays, including ABTS and DDPH scavenging activity, CUPRAC, and FRAP assays. However, the n-hexane extract demonstrated broad inhibitory effects against butyrylcholinesterase, tyrosinase, α-amylase, and α-glucosidase enzymes, supported by molecular docking study and predictive ADME profiling. Therefore, it may be concluded that the n-hexane extract is a viable option for treating dysregulated enzyme conditions. In addition, the potential use of volatile oil in the pharmaceutical industries and management of oxidative stress can be inferred. These results warrant further studies to validate the therapeutic potential of the volatile oil and the n-hexane extract.</p>","PeriodicalId":9878,"journal":{"name":"Chemistry & Biodiversity","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142496052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In recent years, polysaccharides from Codonopsis pilosula (CPs) have received increasing attention for their excellent behaviors in immune-regulation. However, the relationship between the structure and immunomodulatory activity has rarely been reported. In this work, four fractions purified from crude CPs (CPW, CPS0.2, CPS0.5, CPS1) by chromatographic column separation were explored with both structure and immunomodulatory effects by THP-1 cells. The results showed that the monosaccharide composition, chain conformation, molecular weight (Mw) and aggregated state of CPs were different. At the same time, the immunomodulatory effects of CPs were also varied depend on structure differences, as indicated by the released cytokines of TNF-α and IL-1β by LPS-induced THP-1 cells. Finally, we summarized and concluded that the spherical structure and smaller particle size of CPs were the key factors attributed to better immune-enhancing effects. The results showed that the monosaccharide combination differences of polysaccharides might lead to anti-inflammatory or pro-inflammatory activity. Moreover, the higher percentage of glucose, the relatively large particle size, as well as the extended chain conformation of CPs might be the key factors attributed to better immune-enhancing effects. This study aims to provide a theoretical basis for establishing the structure-activity relationship of CPs.
{"title":"The Structure Characterization of Polysaccharides from Codonopsis pilosula and the Structure-activity Relationship with Immune-regulation on THP-1 Cells.","authors":"Junjie Fu, Cong Chang, Jinyang Ren, Jingjing Cheng, Ziwei Gao, Yan Meng","doi":"10.1002/cbdv.202401167","DOIUrl":"https://doi.org/10.1002/cbdv.202401167","url":null,"abstract":"<p><p>In recent years, polysaccharides from Codonopsis pilosula (CPs) have received increasing attention for their excellent behaviors in immune-regulation. However, the relationship between the structure and immunomodulatory activity has rarely been reported. In this work, four fractions purified from crude CPs (CPW, CPS0.2, CPS0.5, CPS1) by chromatographic column separation were explored with both structure and immunomodulatory effects by THP-1 cells. The results showed that the monosaccharide composition, chain conformation, molecular weight (Mw) and aggregated state of CPs were different. At the same time, the immunomodulatory effects of CPs were also varied depend on structure differences, as indicated by the released cytokines of TNF-α and IL-1β by LPS-induced THP-1 cells. Finally, we summarized and concluded that the spherical structure and smaller particle size of CPs were the key factors attributed to better immune-enhancing effects. The results showed that the monosaccharide combination differences of polysaccharides might lead to anti-inflammatory or pro-inflammatory activity. Moreover, the higher percentage of glucose, the relatively large particle size, as well as the extended chain conformation of CPs might be the key factors attributed to better immune-enhancing effects. This study aims to provide a theoretical basis for establishing the structure-activity relationship of CPs.</p>","PeriodicalId":9878,"journal":{"name":"Chemistry & Biodiversity","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142496066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
My Ahmed El Amiri, Hamid Kabdy, Abdelfatah Aitbaba, Jawad Laadraoui, Rachida Aboufatima, Loubna Elyazouli, Baslam Abdelmounaim, Soad Moubtakir, Stefania Garzoli, Chait Abderrahman
Phytotherapy, the use of plant-derived compounds for medicinal purposes, is increasingly recognized as a vital component of modern therapeutics. This study investigates the aqueous extract of Origanum majorana L. (AEOM), evaluating its phytochemical composition, safety, antioxidant activity, and pharmacological effects. Analysis via LC-MS/MS identified a variety of phenolic compounds, with gallic acid, caffeic acid, and chlorogenic acid standing out for their pronounced bioactive properties. Quantitative analysis revealed that AEOM contains significant amounts of polyphenols (186.06 ± 0.1 mg GAE/g), flavonoids (72.3 ± 0.9 mg QE/g), and condensed tannins (4.49 ± 0.08 mg CE/g). The extract exhibited strong antioxidant activity, with an IC₅₀ value of 2.23 ± 0.03 mg/mL in the DPPH assay, and demonstrated considerable reducing power (FRAP value of 1.9 ± 0.01 mg Fe²⁺/g). Pharmacological evaluation showed that AEOM significantly reduced pain in an animal model, suggesting potential analgesic properties. Acute toxicity studies indicated no adverse effects on kidney and liver function or blood parameters at doses up to 800 mg/kg. The analgesic effect is likely mediated by flavonoids in the extract, which may inhibit pain pathways. These findings suggest that O. majorana has promising therapeutic applications, particularly as a natural analgesic agent.
{"title":"Analysis of Chemical Composition, Antioxidant Capacity, Acute Toxicity, and Antinociceptive Properties of Aqueous Extract of Origanum majorana L.","authors":"My Ahmed El Amiri, Hamid Kabdy, Abdelfatah Aitbaba, Jawad Laadraoui, Rachida Aboufatima, Loubna Elyazouli, Baslam Abdelmounaim, Soad Moubtakir, Stefania Garzoli, Chait Abderrahman","doi":"10.1002/cbdv.202401580","DOIUrl":"https://doi.org/10.1002/cbdv.202401580","url":null,"abstract":"<p><p>Phytotherapy, the use of plant-derived compounds for medicinal purposes, is increasingly recognized as a vital component of modern therapeutics. This study investigates the aqueous extract of Origanum majorana L. (AEOM), evaluating its phytochemical composition, safety, antioxidant activity, and pharmacological effects. Analysis via LC-MS/MS identified a variety of phenolic compounds, with gallic acid, caffeic acid, and chlorogenic acid standing out for their pronounced bioactive properties. Quantitative analysis revealed that AEOM contains significant amounts of polyphenols (186.06 ± 0.1 mg GAE/g), flavonoids (72.3 ± 0.9 mg QE/g), and condensed tannins (4.49 ± 0.08 mg CE/g). The extract exhibited strong antioxidant activity, with an IC₅₀ value of 2.23 ± 0.03 mg/mL in the DPPH assay, and demonstrated considerable reducing power (FRAP value of 1.9 ± 0.01 mg Fe²⁺/g). Pharmacological evaluation showed that AEOM significantly reduced pain in an animal model, suggesting potential analgesic properties. Acute toxicity studies indicated no adverse effects on kidney and liver function or blood parameters at doses up to 800 mg/kg. The analgesic effect is likely mediated by flavonoids in the extract, which may inhibit pain pathways. These findings suggest that O. majorana has promising therapeutic applications, particularly as a natural analgesic agent.</p>","PeriodicalId":9878,"journal":{"name":"Chemistry & Biodiversity","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142496047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The current work aims to optimize the ultrasound-assisted extraction (UAE) of Cistus salviifolius L. (aerial parts) antioxidative phenolic compounds using response surface methodology. A Box-Behnken design has been conducted to investigate the effect of four factors, namely: (i) percentage of ethanol (50-90%, v/v), (ii) temperature (40-80°C), (iii) solvent-solid ratio (10-50 mL g-1) and (iv) extraction time (5-25min) on four responses, namely: total phenolic content (TPC), total flavonoid content (TFC) 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical inhibition, and ferric reducing antioxidant power (FRAP). Based on the desirability index, UAE with 50% (v/v) ethanol, at 80 oC, using a solvent-solid ratio of 32.24 mL g-1, for 21 min resulted in the maximum recovery of phenolic antioxidants. Under optimum conditions, the experimental values of TPC, TFC, % DPPH scavenging activity, and FRAP were 171.67 ± 4.69 mg GAE g-1, 26.87 ± 0.78 mg CE g-1, 81.31 ± 0.16%, and 1038.22 ± 7.69 μmol TE g-1, respectively. Results highlight that the developed eco-friendly method is appropriate for the improved recovery of phenolic antioxidants from C. salviifolius L.
本研究旨在利用响应面方法优化肉苁蓉(气生部分)抗氧化酚类化合物的超声辅助萃取(UAE)。采用箱-贝肯设计法研究了四个因素对四个反应的影响,即:(i) 乙醇百分比(50-90%,v/v);(ii) 温度(40-80°C);(iii) 溶剂-固体比率(10-50 mL g-1);(iv) 提取时间(5-25 分钟):总酚含量(TPC)、总黄酮含量(TFC)、2,2-二苯基-1-苦基肼(DPPH)自由基抑制率和铁还原抗氧化能力(FRAP)。根据可取指数,在 80 oC 温度下,用 50%(v/v)乙醇进行超临界提取,溶剂-固体比率为 32.24 mL g-1,时间为 21 分钟,酚类抗氧化剂的回收率最高。在最佳条件下,TPC、TFC、DPPH 清除率和 FRAP 的实验值分别为 171.67 ± 4.69 mg GAE g-1、26.87 ± 0.78 mg CE g-1、81.31 ± 0.16% 和 1038.22 ± 7.69 μmol TE g-1。结果表明,所开发的生态友好型方法适用于提高盐肤木酚类抗氧化剂的回收率。
{"title":"Optimization of the ultrasound-assisted extraction of phenolic antioxidants from Cistus salvifoliusL. using response surface methodology.","authors":"Atalanti Christou, Fotini Nikola, Vlasios Goulas","doi":"10.1002/cbdv.202401337","DOIUrl":"https://doi.org/10.1002/cbdv.202401337","url":null,"abstract":"<p><p>The current work aims to optimize the ultrasound-assisted extraction (UAE) of Cistus salviifolius L. (aerial parts) antioxidative phenolic compounds using response surface methodology. A Box-Behnken design has been conducted to investigate the effect of four factors, namely: (i) percentage of ethanol (50-90%, v/v), (ii) temperature (40-80°C), (iii) solvent-solid ratio (10-50 mL g-1) and (iv) extraction time (5-25min) on four responses, namely: total phenolic content (TPC), total flavonoid content (TFC) 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical inhibition, and ferric reducing antioxidant power (FRAP). Based on the desirability index, UAE with 50% (v/v) ethanol, at 80 oC, using a solvent-solid ratio of 32.24 mL g-1, for 21 min resulted in the maximum recovery of phenolic antioxidants. Under optimum conditions, the experimental values of TPC, TFC, % DPPH scavenging activity, and FRAP were 171.67 ± 4.69 mg GAE g-1, 26.87 ± 0.78 mg CE g-1, 81.31 ± 0.16%, and 1038.22 ± 7.69 μmol TE g-1, respectively. Results highlight that the developed eco-friendly method is appropriate for the improved recovery of phenolic antioxidants from C. salviifolius L.</p>","PeriodicalId":9878,"journal":{"name":"Chemistry & Biodiversity","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142496060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mohammad Asif, Mazen Almehmadi, Mamdouh Allahyani, Meshari A Alsuwat
The low effectiveness of currently available antibiotics is driving efforts worldwide to create new antimicrobial medicines. We made several novel formazan chemicals, including sulfonamides, and assessed how well they may work against bacteria. Consequently, attempts were undertaken to combine the moieties of formazan and sulfonamide to produce new derivatives or more effective antibacterial drugs. Condensing sulphanilamide with benzaldehydes in the presence of glacial acetic acid and ethanol produced a Schiff base of sulphonamide, which when combined with substituted benzene diazonium chlorides (2a-g) during condensation yields formazan derivatives (3a-g). Based on analytical and spectral data (IR, 1HNMR, and mass), the structures of all the synthesized compounds were described, and they agree well with the suggested ones. Using Ciprofloxacin and Ketoconazole as reference medications, the Agar diffusion method was utilized to assess the antibacterial activity of the synthesized compounds by measuring the zone of inhibition against harmful strains of bacteria and fungi. In contrast to the norm, every molecule examined has demonstrated a notable level of antibacterial activity.
{"title":"SYNTHESIS, IN-SILICO BIOCHEMICAL PROPERTIES, AND IN-VITRO ANTIMICROBIAL ACTIVITY OF SOME FARMAZANS DERIVATIVES CONTAINING SULFONAMIDE MOIETY.","authors":"Mohammad Asif, Mazen Almehmadi, Mamdouh Allahyani, Meshari A Alsuwat","doi":"10.1002/cbdv.202402488","DOIUrl":"https://doi.org/10.1002/cbdv.202402488","url":null,"abstract":"<p><p>The low effectiveness of currently available antibiotics is driving efforts worldwide to create new antimicrobial medicines. We made several novel formazan chemicals, including sulfonamides, and assessed how well they may work against bacteria. Consequently, attempts were undertaken to combine the moieties of formazan and sulfonamide to produce new derivatives or more effective antibacterial drugs. Condensing sulphanilamide with benzaldehydes in the presence of glacial acetic acid and ethanol produced a Schiff base of sulphonamide, which when combined with substituted benzene diazonium chlorides (2a-g) during condensation yields formazan derivatives (3a-g). Based on analytical and spectral data (IR, 1HNMR, and mass), the structures of all the synthesized compounds were described, and they agree well with the suggested ones. Using Ciprofloxacin and Ketoconazole as reference medications, the Agar diffusion method was utilized to assess the antibacterial activity of the synthesized compounds by measuring the zone of inhibition against harmful strains of bacteria and fungi. In contrast to the norm, every molecule examined has demonstrated a notable level of antibacterial activity.</p>","PeriodicalId":9878,"journal":{"name":"Chemistry & Biodiversity","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142496065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In order to deeply explore the effect of para-substituents on the antibacterial activity of N-benzyl-3-methylbuten-2- enamide derivatives, we elaborately synthesized three such para-substituted derivatives (compound a: N-(4-hydroxybenzyl)-3-methylbut 2- enamide; compound b: N-(4-isobutoxybenzyl)-3- methylbut-2-enamide; compound c: N-(4-isopropoxybenzyl) -3-methylbut-2- enamide), of which the structures were determined by ways of single crystal X-ray diffraction data analysis mainly. The antibacterial performance experiments showed that compounds a, b and c were evaluated for their antibacterial (Escherichia coli, Staphylococcus aureus, and Enterobacter aerogenes) activities. Among them, compounds a, b and c have an effective antibacterial reagents for E. coli exhibiting MIC values of 0.01, 0.01 and 0.01 g/mL, respectively, but inactive for E. aerogenes. In addition, compounds b and c have better activity than compound a against S. aureus with MIC values of 0.01 and 0.02 g/mL. These results provide an important basis for further study of the antibacterial properties and structure-activity relationship of these compounds, and are expected to provide valuable reference for the development of new antibacterial drugs.
为了深入探讨对位取代基对 N-苄基-3-甲基丁烯-2-烯酰胺衍生物抗菌活性的影响,我们精心合成了三种对位取代基衍生物(化合物 a. N-(4-羟基苄基)-3-甲基丁烯-2-烯酰胺;化合物 b. N-(4-异丁氧基苄基)-3-甲基丁烯-2-烯酰胺;化合物 c. N-(4-异丁氧基苄基)-3-甲基丁烯-2-烯酰胺):N-(4-羟基苄基)-3-甲基丁烯-2-烯酰胺;化合物 b:N-(4-异丁氧基苄基)-3-甲基丁烯-2-烯酰胺;化合物 c:N-(4-异丙氧基苄基)-3-甲基丁烯-2-烯酰胺:化合物b:N-(4-异丁氧基苄基)-3-甲基丁-2-烯酰胺;化合物c:N-(4-异丙氧基苄基)-3-甲基丁-2-烯酰胺,其结构主要通过单晶X射线衍射数据分析确定。抗菌性能实验表明,化合物 a、b 和 c 的抗菌(大肠杆菌、金黄色葡萄球菌和产气肠杆菌)活性得到了评价。其中,化合物 a、b 和 c 对大肠杆菌具有有效的抗菌试剂作用,其 MIC 值分别为 0.01、0.01 和 0.01 g/mL,但对产气肠杆菌无活性。此外,化合物 b 和 c 对金黄色葡萄球菌的活性优于化合物 a,其 MIC 值分别为 0.01 和 0.02 克/毫升。这些结果为进一步研究这些化合物的抗菌特性和结构-活性关系提供了重要依据,有望为开发新的抗菌药物提供有价值的参考。
{"title":"Synthesis, Characterization and Antibacterial Activity Study of Para-substituted Derivatives of N -benzyl-3-methylbuten-2-enamides.","authors":"Wenjie Hu, Yinqiu Liu, Jingyi Yu","doi":"10.1002/cbdv.202402088","DOIUrl":"https://doi.org/10.1002/cbdv.202402088","url":null,"abstract":"<p><p>In order to deeply explore the effect of para-substituents on the antibacterial activity of N-benzyl-3-methylbuten-2- enamide derivatives, we elaborately synthesized three such para-substituted derivatives (compound a: N-(4-hydroxybenzyl)-3-methylbut 2- enamide; compound b: N-(4-isobutoxybenzyl)-3- methylbut-2-enamide; compound c: N-(4-isopropoxybenzyl) -3-methylbut-2- enamide), of which the structures were determined by ways of single crystal X-ray diffraction data analysis mainly. The antibacterial performance experiments showed that compounds a, b and c were evaluated for their antibacterial (Escherichia coli, Staphylococcus aureus, and Enterobacter aerogenes) activities. Among them, compounds a, b and c have an effective antibacterial reagents for E. coli exhibiting MIC values of 0.01, 0.01 and 0.01 g/mL, respectively, but inactive for E. aerogenes. In addition, compounds b and c have better activity than compound a against S. aureus with MIC values of 0.01 and 0.02 g/mL. These results provide an important basis for further study of the antibacterial properties and structure-activity relationship of these compounds, and are expected to provide valuable reference for the development of new antibacterial drugs.</p>","PeriodicalId":9878,"journal":{"name":"Chemistry & Biodiversity","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142496064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
HIV-1 remains a major health problem worldwide since the virus has developed drug-resistant strains, so, the need for novel agents is urgent. The protein reverse transcriptase plays fundamental role in the viruses' replication cycle. FDA approved Delavirdine bearing a sulfonamide moiety, while thiazolidinone has demonstrated significant anti-HIV activity as a core heterocycle or derivative of substituted heterocycles. In this study, thirty new thiazolidinone derivatives (series A, B and C) bearing sulfonamide group were designed, synthesized and evaluated for their HIV-1 RT inhibition activity predicted by computer program PASS taking into account the best features of available NNRTIs as well as against SARS-COV-2 main protease. Seven compounds showed good anti-HIV inhibitory activity, with two of them, C1 and C2 being better (IC50 0.18 μΜ & 0.12 μΜ respectively) than the reference drug nevirapine (IC50 0.31 μΜ). The evaluation of molecules to inhibit the main protease revealed that 6 of the synthesized compounds exhibited excellent to moderate activity with two of them (B4 and B10) having better IC50 values (0.15 & 0.19 μΜ respectively) than the reference inhibitor GC376 (IC50 0.439 μΜ). The docking studies is coincides with experimental results, showing good binding mode to both enzymes.
{"title":"Design, Synthesis, Biological Evaluation and Molecular Docking Studies of New Thiazolidinone Derivatives as NNRTIs and SARS-CoV-2 Main Protease Inhibitors.","authors":"Maria Fesatidou, Anthi Petrou, Athina Geronikaki","doi":"10.1002/cbdv.202401697","DOIUrl":"https://doi.org/10.1002/cbdv.202401697","url":null,"abstract":"<p><p>HIV-1 remains a major health problem worldwide since the virus has developed drug-resistant strains, so, the need for novel agents is urgent. The protein reverse transcriptase plays fundamental role in the viruses' replication cycle. FDA approved Delavirdine bearing a sulfonamide moiety, while thiazolidinone has demonstrated significant anti-HIV activity as a core heterocycle or derivative of substituted heterocycles. In this study, thirty new thiazolidinone derivatives (series A, B and C) bearing sulfonamide group were designed, synthesized and evaluated for their HIV-1 RT inhibition activity predicted by computer program PASS taking into account the best features of available NNRTIs as well as against SARS-COV-2 main protease. Seven compounds showed good anti-HIV inhibitory activity, with two of them, C1 and C2 being better (IC<sub>50</sub> 0.18 μΜ & 0.12 μΜ respectively) than the reference drug nevirapine (IC<sub>50</sub> 0.31 μΜ). The evaluation of molecules to inhibit the main protease revealed that 6 of the synthesized compounds exhibited excellent to moderate activity with two of them (B4 and B10) having better IC<sub>50</sub> values (0.15 & 0.19 μΜ respectively) than the reference inhibitor GC376 (IC<sub>50</sub> 0.439 μΜ). The docking studies is coincides with experimental results, showing good binding mode to both enzymes.</p>","PeriodicalId":9878,"journal":{"name":"Chemistry & Biodiversity","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142496053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ngoc Van Nguyen, Nhi Ngoc Uyen Tran, Binh Thi Hoang
An analysis was conducted on the essential oil extracted from the leaves of Magnolia bidoupensis utilizing GC-MS, revealing thirty-three constituents that account for 98.9% of the essential oil. The main components included pogostol (22.4%), δ-selinene (16.2%), and α-amorphene (14.7%). Bioassays were then performed to evaluate the oil's biological activity. The essential oil exhibited antimicrobial activity against all tested microorganisms (six bacterial strains and one fungal strain) using the minimum inhibitory concentration (MIC) method. Additional cytotoxicity tests were conducted on KB, HepG2, MCF-7, and A549 cancer cell lines using the MTT method. The essential oil exhibited strong cytotoxic effects on all four cell lines, with IC50 values ranging from 1.37 ± 0.05 μg/mL (KB) to 2.40 ± 0.06 μg/mL (A549).
{"title":"Chemical Composition, Anti-microbial, and Cytotoxic Activities of Essential Oil from Magnolia bidoupensis Q. N. Vu leaves, an Endemic Species to the Central Highlands of Vietnam.","authors":"Ngoc Van Nguyen, Nhi Ngoc Uyen Tran, Binh Thi Hoang","doi":"10.1002/cbdv.202402472","DOIUrl":"https://doi.org/10.1002/cbdv.202402472","url":null,"abstract":"<p><p>An analysis was conducted on the essential oil extracted from the leaves of Magnolia bidoupensis utilizing GC-MS, revealing thirty-three constituents that account for 98.9% of the essential oil. The main components included pogostol (22.4%), δ-selinene (16.2%), and α-amorphene (14.7%). Bioassays were then performed to evaluate the oil's biological activity. The essential oil exhibited antimicrobial activity against all tested microorganisms (six bacterial strains and one fungal strain) using the minimum inhibitory concentration (MIC) method. Additional cytotoxicity tests were conducted on KB, HepG2, MCF-7, and A549 cancer cell lines using the MTT method. The essential oil exhibited strong cytotoxic effects on all four cell lines, with IC50 values ranging from 1.37 ± 0.05 μg/mL (KB) to 2.40 ± 0.06 μg/mL (A549).</p>","PeriodicalId":9878,"journal":{"name":"Chemistry & Biodiversity","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142496051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}