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Assessment of Artemisia campestris L. Leaf Extract Effects on Polycystic Ovarian Syndrome in Rats, Antioxidant and α-Amylase Inhibition Activities. 评估青蒿叶提取物对大鼠多囊卵巢综合征的影响、抗氧化和α-淀粉酶抑制活性
IF 2.3 3区 化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-10-27 DOI: 10.1002/cbdv.202402184
Inasse Cherfi, Nasma Mahboub, Ikram Toumi, Salah Eddine Laouini, Gamil Gamal Hasan, Abderrhmane Bouafia, Fahad Alharthi, Talha Bin Emran

Polycystic Ovarian Syndrome (PCOS) is characterized by metabolic and reproductive dysfunction, often associated with elevated oxidative stress markers in the bloodstream. This study examines the potential antioxidant properties and α-amylase inhibitory activity of Artemisia campestris leaves extract (Artemisia campestris L) and its effects on rats with induced PCOS. Estradiol valerate was administered to ten mature Wistar rats to induce PCOS, while a control group consisted of five mature Wistar rats. Following a 16-day induction period, the rats were categorized into three groups: a control group, a PCOS group, and an experimental group receiving 200 mg/kg body weight of A. campestris L. extract orally for 15 days. Serum levels of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) were measured using the ELISA technique. The group treated with A. campestris L. extract exhibited significantly reduced LH levels compared to the PCOS group. Histomorphometric analysis indicated notable changes in follicle counts and the thickness of the theca layer. These findings suggest a significant alleviation of PCOS symptoms, potentially linked to the effects of A. campestris L. on oxidative stress pathways. Furthermore, aqueous extracts of A. campestris L. displayed potent in vitro inhibition of α-amylase, with an IC50 value of 2.418 µg/mL.

多囊卵巢综合征(PCOS)以代谢和生殖功能障碍为特征,通常与血液中氧化应激指标升高有关。本研究探讨了青蒿叶提取物(青蒿 L)的潜在抗氧化特性、α-淀粉酶抑制活性及其对诱发多囊卵巢综合征大鼠的影响。对 10 只成熟的 Wistar 大鼠施用戊酸雌二醇诱导多囊卵巢综合征,对照组由 5 只成熟的 Wistar 大鼠组成。经过 16 天的诱导期后,大鼠被分为三组:对照组、多囊卵巢综合征组和连续 15 天口服 200 毫克/公斤体重的 A. campestris L. 提取物的实验组。采用 ELISA 技术测定血清中的卵泡刺激素(FSH)和黄体生成素(LH)水平。与多囊卵巢综合征组相比,接受 A. campestris L. 提取物治疗的组的 LH 水平明显降低。组织形态分析表明,卵泡数量和子宫颈层厚度发生了明显变化。这些研究结果表明,多囊卵巢综合症的症状明显减轻,这可能与 A. campestris L. 对氧化应激途径的影响有关。此外,A. campestris L.的水提取物对α-淀粉酶有很强的体外抑制作用,其 IC50 值为 2.418 µg/mL。
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引用次数: 0
Novel Insecticidal Benzo[4,5]imidazo[1,2-b]pyrazole Derivatives Identified through Ring-Closure Scaffold Hopping on Fipronil. 通过氟虫腈上的闭环支架跳跃发现的新型杀虫苯并[4,5]咪唑并[1,2-b]吡唑衍生物。
IF 2.3 3区 化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-10-27 DOI: 10.1002/cbdv.202402148
Cong Zhou, Guanglong Li, Sihui Wang, Zhong Li, Xuhong Qian, Jiagao Cheng

A series of innovative benzo[4,5]imidazo[1,2-b]pyrazole scaffold containing compounds were rationally designed through a ring-closure scaffold hopping strategy and synthetized with an intermediate derivatization approach. Physicochemical properties analysis indicated the potential pesticide-likeness of the target compounds. The optimal target compound A14 showed relatively good insecticidal activity against P. xylostella, with an LC50 value of 37.58 mg/L, and demonstrated lower acute fish toxicity compared to fipronil. Docking binding mode analysis demonstrated that compound A14 bound to GABAR through a H-bond between the amide group and the residue of 6'Thr. The differences in binding modes between benzo[4,5]imidazo[1,2-b]pyrazole target compounds and fipronil may be a key factor for the reduced insecticidal activities. The elucidated binding mode and SAR profile lay a foundation for the further structural optimization of insecticidal benzo[4,5]imidazo[1,2-b]pyrazole derivatives.

通过环闭支架跳转策略合理设计了一系列含有苯并[4,5]咪唑并[1,2-b]吡唑支架的创新化合物,并采用中间体衍生化方法合成了这些化合物。理化性质分析表明,目标化合物具有潜在的农药相似性。最佳目标化合物 A14 对木虱具有较好的杀虫活性,半数致死浓度为 37.58 毫克/升,与氟虫腈相比,其对鱼类的急性毒性更低。Docking 结合模式分析表明,化合物 A14 通过酰胺基团与 6'Thr 残基之间的 H 键与 GABAR 结合。苯并[4,5]咪唑并[1,2-b]吡唑目标化合物与氟虫腈结合模式的差异可能是导致杀虫活性降低的关键因素。阐明的结合模式和 SAR 特征为进一步优化杀虫苯并[4,5]咪唑并[1,2-b]吡唑衍生物的结构奠定了基础。
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引用次数: 0
Chemical Constituents, Antioxidant, and Enzyme Inhibitory Potentials Supported by In-Silico Studies of the n-Hexane Extract and Essential Oil of Platycladus orientalis (L.) Franco Leaves. 通过对 Platycladus orientalis (L.) Franco 叶的正己烷萃取物和精油的化学成分、抗氧化性和酶抑制潜能的分子内研究。
IF 2.3 3区 化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-10-27 DOI: 10.1002/cbdv.202402000
Doaa Ahmed Korany, Nilofar Nilofar, Gokhan Zengin, Omayma A Eldahshan

Platycladus orientalis leaves are widely used in traditional medicine to treat different ailments. In the present study, the volatile constituents were obtained by n-hexane extraction and hydrodistillation. Comprehensive metabolomic profiling was performed using GC-MS analysis. Furthermore, in vitro antioxidant potential and enzyme-inhibitory activity were assessed and supported by in silico profiling. Results revealed the predominance of monoterpene hydrocarbons in the hydrodistilled volatile oil (42.30%) followed by oxygenated sesquiterpenes (32.10%); with cedrol as the main component. Diterpenoids (49.70%) and sesquiterpene hydrocarbons (13.43%) were the major components of the n-hexane extract; with vulgarol A, a diterpene alcohol, as the major constituent. The volatile oil demonstrated significantly higher antioxidant potential across all assays, including ABTS and DDPH scavenging activity, CUPRAC, and FRAP assays. However, the n-hexane extract demonstrated broad inhibitory effects against butyrylcholinesterase, tyrosinase, α-amylase, and α-glucosidase enzymes, supported by molecular docking study and predictive ADME profiling. Therefore, it may be concluded that the n-hexane extract is a viable option for treating dysregulated enzyme conditions. In addition, the potential use of volatile oil in the pharmaceutical industries and management of oxidative stress can be inferred. These results warrant further studies to validate the therapeutic potential of the volatile oil and the n-hexane extract.

板蓝根叶在传统医学中被广泛用于治疗各种疾病。本研究通过正己烷萃取和水蒸馏法获得了挥发性成分。利用气相色谱-质谱分析进行了全面的代谢组学分析。此外,还对体外抗氧化潜力和酶抑制活性进行了评估,并得到了硅学分析的支持。结果表明,水蒸馏挥发油中主要是单萜烃类(42.30%),其次是含氧倍半萜类(32.10%);主要成分是柏木酚。二萜类化合物(49.70%)和倍半萜碳氢化合物(13.43%)是正己烷萃取物的主要成分;二萜醇 vulgarol A 是主要成分。挥发油在 ABTS 和 DDPH 清除活性、CUPRAC 和 FRAP 试验等所有试验中都表现出更高的抗氧化潜力。不过,正己烷提取物对丁酰胆硷酯酶、酪氨酸酶、α-淀粉酶和α-葡萄糖苷酶具有广泛的抑制作用,分子对接研究和预测性 ADME 分析也证明了这一点。因此,可以得出结论,正己烷提取物是治疗酶失调症的可行选择。此外,还可以推断出挥发油在制药业和氧化应激管理方面的潜在用途。这些结果值得进一步研究,以验证挥发油和正己烷提取物的治疗潜力。
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引用次数: 0
The Structure Characterization of Polysaccharides from Codonopsis pilosula and the Structure-activity Relationship with Immune-regulation on THP-1 Cells. 党参多糖的结构特征及与 THP-1 细胞免疫调节的结构-活性关系
IF 2.3 3区 化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-10-25 DOI: 10.1002/cbdv.202401167
Junjie Fu, Cong Chang, Jinyang Ren, Jingjing Cheng, Ziwei Gao, Yan Meng

In recent years, polysaccharides from Codonopsis pilosula (CPs) have received increasing attention for their excellent behaviors in immune-regulation. However, the relationship between the structure and immunomodulatory activity has rarely been reported. In this work, four fractions purified from crude CPs (CPW, CPS0.2, CPS0.5, CPS1) by chromatographic column separation were explored with both structure and immunomodulatory effects by THP-1 cells. The results showed that the monosaccharide composition, chain conformation, molecular weight (Mw) and aggregated state of CPs were different. At the same time, the immunomodulatory effects of CPs were also varied depend on structure differences, as indicated by the released cytokines of TNF-α and IL-1β by LPS-induced THP-1 cells. Finally, we summarized and concluded that the spherical structure and smaller particle size of CPs were the key factors attributed to better immune-enhancing effects. The results showed that the monosaccharide combination differences of polysaccharides might lead to anti-inflammatory or pro-inflammatory activity. Moreover, the higher percentage of glucose, the relatively large particle size, as well as the extended chain conformation of CPs might be the key factors attributed to better immune-enhancing effects. This study aims to provide a theoretical basis for establishing the structure-activity relationship of CPs.

近年来,党参多糖因其在免疫调节方面的卓越表现而受到越来越多的关注。然而,其结构与免疫调节活性之间的关系却鲜有报道。本研究以 THP-1 细胞为研究对象,通过色谱柱分离纯化了四种粗提物(CPW、CPS0.2、CPS0.5、CPS1),探讨了它们的结构和免疫调节作用。结果表明,CPs 的单糖组成、链构象、分子量(Mw)和聚集状态各不相同。同时,CPs 的免疫调节作用也因结构差异而不同,LPS 诱导的 THP-1 细胞释放的 TNF-α 和 IL-1β 细胞因子也表明了这一点。最后,我们总结并得出结论:CPs 的球形结构和较小的粒径是其具有较好免疫增强效果的关键因素。结果表明,多糖的单糖组合差异可能会导致抗炎或促炎活性。此外,CPs 中较高的葡萄糖比例、相对较大的粒径以及延链构象可能是其具有较好免疫增强效果的关键因素。本研究旨在为建立氯化石蜡的结构-活性关系提供理论依据。
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引用次数: 0
Analysis of Chemical Composition, Antioxidant Capacity, Acute Toxicity, and Antinociceptive Properties of Aqueous Extract of Origanum majorana L. 牛至水提取物的化学成分、抗氧化能力、急性毒性和抗痛性分析
IF 2.3 3区 化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-10-24 DOI: 10.1002/cbdv.202401580
My Ahmed El Amiri, Hamid Kabdy, Abdelfatah Aitbaba, Jawad Laadraoui, Rachida Aboufatima, Loubna Elyazouli, Baslam Abdelmounaim, Soad Moubtakir, Stefania Garzoli, Chait Abderrahman

Phytotherapy, the use of plant-derived compounds for medicinal purposes, is increasingly recognized as a vital component of modern therapeutics. This study investigates the aqueous extract of Origanum majorana L. (AEOM), evaluating its phytochemical composition, safety, antioxidant activity, and pharmacological effects. Analysis via LC-MS/MS identified a variety of phenolic compounds, with gallic acid, caffeic acid, and chlorogenic acid standing out for their pronounced bioactive properties. Quantitative analysis revealed that AEOM contains significant amounts of polyphenols (186.06 ± 0.1 mg GAE/g), flavonoids (72.3 ± 0.9 mg QE/g), and condensed tannins (4.49 ± 0.08 mg CE/g). The extract exhibited strong antioxidant activity, with an IC₅₀ value of 2.23 ± 0.03 mg/mL in the DPPH assay, and demonstrated considerable reducing power (FRAP value of 1.9 ± 0.01 mg Fe²⁺/g). Pharmacological evaluation showed that AEOM significantly reduced pain in an animal model, suggesting potential analgesic properties. Acute toxicity studies indicated no adverse effects on kidney and liver function or blood parameters at doses up to 800 mg/kg. The analgesic effect is likely mediated by flavonoids in the extract, which may inhibit pain pathways. These findings suggest that O. majorana has promising therapeutic applications, particularly as a natural analgesic agent.

植物疗法是将植物提取的化合物用于医疗目的,越来越被认为是现代疗法的重要组成部分。本研究调查了牛至(Origanum majorana L.,AEOM)的水提取物,评估了其植物化学成分、安全性、抗氧化活性和药理作用。通过 LC-MS/MS 分析,确定了多种酚类化合物,其中没食子酸、咖啡酸和绿原酸具有明显的生物活性。定量分析显示,AEOM 含有大量多酚(186.06 ± 0.1 毫克 GAE/克)、类黄酮(72.3 ± 0.9 毫克 QE/克)和缩合单宁(4.49 ± 0.08 毫克 CE/克)。该提取物具有很强的抗氧化活性,在 DPPH 试验中的 IC₅₀值为 2.23 ± 0.03 mg/mL,并表现出相当强的还原力(FRAP 值为 1.9 ± 0.01 mg Fe²⁺/g)。药理评估显示,AEOM 能明显减轻动物模型的疼痛,这表明它具有潜在的镇痛特性。急性毒性研究表明,在剂量高达 800 毫克/千克时,AEOM 不会对肝肾功能或血液参数产生不良影响。镇痛效果可能是由提取物中的类黄酮介导的,它可能会抑制疼痛通路。这些研究结果表明,O. majorana 具有广阔的治疗应用前景,尤其是作为一种天然镇痛剂。
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引用次数: 0
Optimization of the ultrasound-assisted extraction of phenolic antioxidants from Cistus salvifoliusL. using response surface methodology. 利用响应面方法优化超声辅助提取肉苁蓉中的酚类抗氧化剂。
IF 2.3 3区 化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-10-24 DOI: 10.1002/cbdv.202401337
Atalanti Christou, Fotini Nikola, Vlasios Goulas

The current work aims to optimize the ultrasound-assisted extraction (UAE) of Cistus salviifolius L. (aerial parts) antioxidative phenolic compounds using response surface methodology. A Box-Behnken design has been conducted to investigate the effect of four factors, namely: (i) percentage of ethanol (50-90%, v/v), (ii) temperature (40-80°C), (iii) solvent-solid ratio (10-50 mL g-1) and (iv) extraction time (5-25min) on four responses, namely: total phenolic content (TPC), total flavonoid content (TFC) 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical inhibition, and ferric reducing antioxidant power (FRAP). Based on the desirability index, UAE with 50% (v/v) ethanol, at 80 oC, using a solvent-solid ratio of 32.24 mL g-1, for 21 min resulted in the maximum recovery of phenolic antioxidants. Under optimum conditions, the experimental values of TPC, TFC, % DPPH scavenging activity, and FRAP were 171.67 ± 4.69 mg GAE g-1, 26.87 ± 0.78 mg CE g-1, 81.31 ±  0.16%, and 1038.22 ± 7.69 μmol TE g-1, respectively. Results highlight that the developed eco-friendly method is appropriate for the improved recovery of phenolic antioxidants from C. salviifolius L.

本研究旨在利用响应面方法优化肉苁蓉(气生部分)抗氧化酚类化合物的超声辅助萃取(UAE)。采用箱-贝肯设计法研究了四个因素对四个反应的影响,即:(i) 乙醇百分比(50-90%,v/v);(ii) 温度(40-80°C);(iii) 溶剂-固体比率(10-50 mL g-1);(iv) 提取时间(5-25 分钟):总酚含量(TPC)、总黄酮含量(TFC)、2,2-二苯基-1-苦基肼(DPPH)自由基抑制率和铁还原抗氧化能力(FRAP)。根据可取指数,在 80 oC 温度下,用 50%(v/v)乙醇进行超临界提取,溶剂-固体比率为 32.24 mL g-1,时间为 21 分钟,酚类抗氧化剂的回收率最高。在最佳条件下,TPC、TFC、DPPH 清除率和 FRAP 的实验值分别为 171.67 ± 4.69 mg GAE g-1、26.87 ± 0.78 mg CE g-1、81.31 ± 0.16% 和 1038.22 ± 7.69 μmol TE g-1。结果表明,所开发的生态友好型方法适用于提高盐肤木酚类抗氧化剂的回收率。
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引用次数: 0
SYNTHESIS, IN-SILICO BIOCHEMICAL PROPERTIES, AND IN-VITRO ANTIMICROBIAL ACTIVITY OF SOME FARMAZANS DERIVATIVES CONTAINING SULFONAMIDE MOIETY. 一些含磺酰胺分子的法马赞衍生物的合成、体内生化性质和体外抗菌活性。
IF 2.3 3区 化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-10-24 DOI: 10.1002/cbdv.202402488
Mohammad Asif, Mazen Almehmadi, Mamdouh Allahyani, Meshari A Alsuwat

The low effectiveness of currently available antibiotics is driving efforts worldwide to create new antimicrobial medicines. We made several novel formazan chemicals, including sulfonamides, and assessed how well they may work against bacteria. Consequently, attempts were undertaken to combine the moieties of formazan and sulfonamide to produce new derivatives or more effective antibacterial drugs. Condensing sulphanilamide with benzaldehydes in the presence of glacial acetic acid and ethanol produced a Schiff base of sulphonamide, which when combined with substituted benzene diazonium chlorides (2a-g) during condensation yields formazan derivatives (3a-g). Based on analytical and spectral data (IR, 1HNMR, and mass), the structures of all the synthesized compounds were described, and they agree well with the suggested ones. Using Ciprofloxacin and Ketoconazole as reference medications, the Agar diffusion method was utilized to assess the antibacterial activity of the synthesized compounds by measuring the zone of inhibition against harmful strains of bacteria and fungi. In contrast to the norm, every molecule examined has demonstrated a notable level of antibacterial activity.

现有抗生素疗效不佳,促使全世界都在努力开发新的抗菌药物。我们制造了几种新型的蚁酸化学物质,包括磺酰胺,并评估了它们对细菌的作用。因此,我们尝试将甲臢和磺酰胺的分子结合起来,生产出新的衍生物或更有效的抗菌药物。磺胺与苯甲醛在冰醋酸和乙醇存在下缩合,产生磺酰胺的希夫碱,在缩合过程中与取代的苯重氮氯化物(2a-g)结合,产生甲臢衍生物(3a-g)。根据分析和光谱数据(红外光谱、1HNMR 和质量),描述了所有合成化合物的结构,这些结构与建议的结构非常吻合。以环丙沙星和酮康唑为参照药物,采用琼脂扩散法测量合成化合物对有害细菌和真菌菌株的抑制区,以评估其抗菌活性。与常规方法不同的是,所研究的每个分子都表现出了显著的抗菌活性。
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引用次数: 0
Synthesis, Characterization and Antibacterial Activity Study of Para-substituted Derivatives of N -benzyl-3-methylbuten-2-enamides. N -苄基-3-甲基丁烯-2-烯酰胺对取代衍生物的合成、表征和抗菌活性研究。
IF 2.3 3区 化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-10-24 DOI: 10.1002/cbdv.202402088
Wenjie Hu, Yinqiu Liu, Jingyi Yu

In order to deeply explore the effect of para-substituents on the antibacterial activity of N-benzyl-3-methylbuten-2- enamide derivatives, we elaborately synthesized three such para-substituted derivatives (compound a: N-(4-hydroxybenzyl)-3-methylbut 2- enamide; compound b: N-(4-isobutoxybenzyl)-3- methylbut-2-enamide; compound c: N-(4-isopropoxybenzyl) -3-methylbut-2- enamide), of which the structures were determined by ways of single crystal X-ray diffraction data analysis mainly. The antibacterial performance experiments showed that compounds a, b and c were evaluated for their antibacterial (Escherichia coli, Staphylococcus aureus, and Enterobacter aerogenes) activities. Among them, compounds a, b and c have an effective antibacterial reagents for E. coli exhibiting MIC values of 0.01, 0.01 and 0.01 g/mL, respectively, but inactive for E. aerogenes. In addition, compounds b and c have better activity than compound a against S. aureus with MIC values of 0.01 and 0.02 g/mL. These results provide an important basis for further study of the antibacterial properties and structure-activity relationship of these compounds, and are expected to provide valuable reference for the development of new antibacterial drugs.

为了深入探讨对位取代基对 N-苄基-3-甲基丁烯-2-烯酰胺衍生物抗菌活性的影响,我们精心合成了三种对位取代基衍生物(化合物 a. N-(4-羟基苄基)-3-甲基丁烯-2-烯酰胺;化合物 b. N-(4-异丁氧基苄基)-3-甲基丁烯-2-烯酰胺;化合物 c. N-(4-异丁氧基苄基)-3-甲基丁烯-2-烯酰胺):N-(4-羟基苄基)-3-甲基丁烯-2-烯酰胺;化合物 b:N-(4-异丁氧基苄基)-3-甲基丁烯-2-烯酰胺;化合物 c:N-(4-异丙氧基苄基)-3-甲基丁烯-2-烯酰胺:化合物b:N-(4-异丁氧基苄基)-3-甲基丁-2-烯酰胺;化合物c:N-(4-异丙氧基苄基)-3-甲基丁-2-烯酰胺,其结构主要通过单晶X射线衍射数据分析确定。抗菌性能实验表明,化合物 a、b 和 c 的抗菌(大肠杆菌、金黄色葡萄球菌和产气肠杆菌)活性得到了评价。其中,化合物 a、b 和 c 对大肠杆菌具有有效的抗菌试剂作用,其 MIC 值分别为 0.01、0.01 和 0.01 g/mL,但对产气肠杆菌无活性。此外,化合物 b 和 c 对金黄色葡萄球菌的活性优于化合物 a,其 MIC 值分别为 0.01 和 0.02 克/毫升。这些结果为进一步研究这些化合物的抗菌特性和结构-活性关系提供了重要依据,有望为开发新的抗菌药物提供有价值的参考。
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引用次数: 0
Design, Synthesis, Biological Evaluation and Molecular Docking Studies of New Thiazolidinone Derivatives as NNRTIs and SARS-CoV-2 Main Protease Inhibitors. 作为 NNRTIs 和 SARS-CoV-2 主要蛋白酶抑制剂的新型噻唑烷酮衍生物的设计、合成、生物学评价和分子对接研究
IF 2.3 3区 化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-10-23 DOI: 10.1002/cbdv.202401697
Maria Fesatidou, Anthi Petrou, Athina Geronikaki

HIV-1 remains a major health problem worldwide since the virus has developed drug-resistant strains, so, the need for novel agents is urgent. The protein reverse transcriptase plays fundamental role in the viruses' replication cycle. FDA approved Delavirdine bearing a sulfonamide moiety, while thiazolidinone has demonstrated significant anti-HIV activity as a core heterocycle or derivative of substituted heterocycles. In this study, thirty new thiazolidinone derivatives (series A, B and C) bearing sulfonamide group were designed, synthesized and evaluated for their HIV-1 RT inhibition activity predicted by computer program PASS taking into account the best features of available NNRTIs as well as against SARS-COV-2 main protease. Seven compounds showed good anti-HIV inhibitory activity, with two of them, C1 and C2 being better (IC50 0.18 μΜ & 0.12 μΜ respectively) than the reference drug nevirapine (IC50 0.31 μΜ). The evaluation of molecules to inhibit the main protease revealed that 6 of the synthesized compounds exhibited excellent to moderate activity with two of them (B4 and B10) having better IC50 values (0.15 & 0.19 μΜ respectively) than the reference inhibitor GC376 (IC50 0.439 μΜ). The docking studies is coincides with experimental results, showing good binding mode to both enzymes.

由于 HIV-1 病毒已发展出耐药株,因此它仍然是全球的一个主要健康问题,对新型药物的需求十分迫切。蛋白质逆转录酶在病毒的复制周期中起着根本性的作用。美国食品和药物管理局批准了含有磺酰胺分子的 Delavirdine,而噻唑烷酮作为核心杂环或取代杂环的衍生物,已被证明具有显著的抗艾滋病毒活性。本研究设计、合成了 30 种带有磺酰胺基团的新噻唑烷酮衍生物(A、B 和 C 系列),并通过计算机程序 PASS 预测评估了它们对 HIV-1 RT 的抑制活性,其中考虑到了现有 NNRTIs 的最佳特性以及对 SARS-COV-2 主要蛋白酶的抑制活性。七个化合物显示出良好的抗艾滋病毒抑制活性,其中两个化合物 C1 和 C2(IC50 分别为 0.18 μΜ 和 0.12 μΜ)优于参考药物奈韦拉平(IC50 0.31 μΜ)。对抑制主要蛋白酶的分子进行评估后发现,6 个合成化合物具有极佳至中等程度的活性,其中两个(B4 和 B10)的 IC50 值(分别为 0.15 和 0.19 μΜ)优于参考抑制剂 GC376(IC50 0.439 μΜ)。对接研究与实验结果相吻合,显示了与两种酶的良好结合模式。
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引用次数: 0
Chemical Composition, Anti-microbial, and Cytotoxic Activities of Essential Oil from Magnolia bidoupensis Q. N. Vu leaves, an Endemic Species to the Central Highlands of Vietnam. 越南中部高原特有种木兰(Magnolia bidoupensis Q. N. Vu)叶中精油的化学成分、抗微生物和细胞毒性活性。
IF 2.3 3区 化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-10-23 DOI: 10.1002/cbdv.202402472
Ngoc Van Nguyen, Nhi Ngoc Uyen Tran, Binh Thi Hoang

An analysis was conducted on the essential oil extracted from the leaves of Magnolia bidoupensis utilizing GC-MS, revealing thirty-three constituents that account for 98.9% of the essential oil. The main components included pogostol (22.4%), δ-selinene (16.2%), and α-amorphene (14.7%). Bioassays were then performed to evaluate the oil's biological activity. The essential oil exhibited antimicrobial activity against all tested microorganisms (six bacterial strains and one fungal strain) using the minimum inhibitory concentration (MIC) method. Additional cytotoxicity tests were conducted on KB, HepG2, MCF-7, and A549 cancer cell lines using the MTT method. The essential oil exhibited strong cytotoxic effects on all four cell lines, with IC50 values ranging from 1.37 ± 0.05 μg/mL (KB) to 2.40 ± 0.06 μg/mL (A549).

利用气相色谱-质谱(GC-MS)对从白玉兰(Magnolia bidoupensis)叶片中提取的精油进行了分析,发现其中有 33 种成分,占精油的 98.9%。主要成分包括 pogostol(22.4%)、δ-selinene(16.2%)和 α-amorphene(14.7%)。然后进行了生物测定,以评估精油的生物活性。采用最小抑菌浓度(MIC)法,精油对所有受测微生物(六种细菌菌株和一种真菌菌株)都具有抗菌活性。此外,还采用 MTT 法对 KB、HepG2、MCF-7 和 A549 癌细胞系进行了细胞毒性测试。精油对所有四种细胞株都有很强的细胞毒性作用,IC50 值从 1.37 ± 0.05 μg/mL(KB)到 2.40 ± 0.06 μg/mL(A549)不等。
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引用次数: 0
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Chemistry & Biodiversity
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