Pub Date : 2025-06-01Epub Date: 2025-01-18DOI: 10.1016/j.asems.2025.100135
Min Yang , Yushi Xie , Longjiao Zhu , Ran Wang , Jie Zheng , Wentao Xu
Biogenic amines (BAs) are a class of small nitrogen-containing organic compounds commonly found in various foods and are one of the common metabolic by-products in the process of food spoilage. When consumed in excessive amounts by the human body, BAs can cause a range of adverse reactions such as difficulty in breathing and palpitations, posing a serious threat to life and health. Moreover, the content of BAs is closely related to the degree of food spoilage, making them an important indicator for measuring food quality and freshness. Therefore, accurate detection of BAs is particularly important. Aptamer biosensors are becoming more and more important in the field of biosensing and show great potential. In this review, we first systematically summarized the structural characteristics, formation mechanism and potential toxicity of BAs. Then, the screening strategies and methods of biogenic amine aptamers were discussed. On this basis, we focus on the latest progress in the field of aptamer sensor technology for BAs detection in food and divide these technologies into four categories according to the detection principle: colorimetric analysis, fluorescence detection, surface-enhanced Raman spectroscopy (SERS) analysis and electrochemical detection. Finally, the future development direction and current challenges of biogenic amine detection strategies are introduced.
{"title":"Aptamer-based biosensors for biogenic amines detection","authors":"Min Yang , Yushi Xie , Longjiao Zhu , Ran Wang , Jie Zheng , Wentao Xu","doi":"10.1016/j.asems.2025.100135","DOIUrl":"10.1016/j.asems.2025.100135","url":null,"abstract":"<div><div>Biogenic amines (BAs) are a class of small nitrogen-containing organic compounds commonly found in various foods and are one of the common metabolic by-products in the process of food spoilage. When consumed in excessive amounts by the human body, BAs can cause a range of adverse reactions such as difficulty in breathing and palpitations, posing a serious threat to life and health. Moreover, the content of BAs is closely related to the degree of food spoilage, making them an important indicator for measuring food quality and freshness. Therefore, accurate detection of BAs is particularly important. Aptamer biosensors are becoming more and more important in the field of biosensing and show great potential. In this review, we first systematically summarized the structural characteristics, formation mechanism and potential toxicity of BAs. Then, the screening strategies and methods of biogenic amine aptamers were discussed. On this basis, we focus on the latest progress in the field of aptamer sensor technology for BAs detection in food and divide these technologies into four categories according to the detection principle: colorimetric analysis, fluorescence detection, surface-enhanced Raman spectroscopy (SERS) analysis and electrochemical detection. Finally, the future development direction and current challenges of biogenic amine detection strategies are introduced.</div></div>","PeriodicalId":100036,"journal":{"name":"Advanced Sensor and Energy Materials","volume":"4 2","pages":"Article 100135"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143600909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-06-01Epub Date: 2025-03-01DOI: 10.1016/j.asems.2025.100143
Chengyi Xiong
{"title":"Editorial: Recent approaches in biosensors and wearable sensors","authors":"Chengyi Xiong","doi":"10.1016/j.asems.2025.100143","DOIUrl":"10.1016/j.asems.2025.100143","url":null,"abstract":"","PeriodicalId":100036,"journal":{"name":"Advanced Sensor and Energy Materials","volume":"4 2","pages":"Article 100143"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143898761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-06-01Epub Date: 2025-03-03DOI: 10.1016/j.asems.2025.100141
Ke Quan , Yuqing Zeng , Sijia Gao , Yanli Lei , Le Yang , Yibo Zhou , Lucky Poh Wah Goh , Zhihe Qing
The prevalent oral diseases, such as dental caries, chronic gingivitis, and periodontitis, which are primarily caused by pathogenic bacteria, pose significant public health risks and impose substantial economic burdens. However, conventional treatment strategies for oral pathogens rely on mechanical debridement and antibiotic treatment, which remain unsatisfactory and contribute to the emergence of antimicrobial resistance pathogens. The escalating crisis of antibiotic resistance and the intricate microbial communities in oral niches urgently demand innovative antimicrobial strategies that can overcome these issues. Metal-bearing nanomaterials (MBNs), as an integration of metallic components with other substances such as polymers or inorganic materials, have demonstrated improved antimicrobial effectiveness while mitigating the toxicity associated with pure metals in oral environments. This review provides an innovative overview of designing and utilizing MBNs for oral antimicrobial applications, bridging the gap between nanomaterial design and clinical dentistry needs while guiding the development of next-generation antimicrobials in the post-antibiotic era. Firstly, we categorize and elucidate the main antibacterial mechanisms of metallic components in MBNs. Furthermore, a comprehensive summary is provided on the up-to-date advancements in using MBNs for oral antibacterial purposes, highlighting the pivotal role of metals in enhancing antibacterial properties. Finally, we discuss the existing challenges and potential future developments to establish a theoretical foundation for ongoing progress and clinical approval.
{"title":"Metal-bearing nanomaterials for oral antibacteria: Mechanisms and applications","authors":"Ke Quan , Yuqing Zeng , Sijia Gao , Yanli Lei , Le Yang , Yibo Zhou , Lucky Poh Wah Goh , Zhihe Qing","doi":"10.1016/j.asems.2025.100141","DOIUrl":"10.1016/j.asems.2025.100141","url":null,"abstract":"<div><div>The prevalent oral diseases, such as dental caries, chronic gingivitis, and periodontitis, which are primarily caused by pathogenic bacteria, pose significant public health risks and impose substantial economic burdens. However, conventional treatment strategies for oral pathogens rely on mechanical debridement and antibiotic treatment, which remain unsatisfactory and contribute to the emergence of antimicrobial resistance pathogens. The escalating crisis of antibiotic resistance and the intricate microbial communities in oral niches urgently demand innovative antimicrobial strategies that can overcome these issues. Metal-bearing nanomaterials (MBNs), as an integration of metallic components with other substances such as polymers or inorganic materials, have demonstrated improved antimicrobial effectiveness while mitigating the toxicity associated with pure metals in oral environments. This review provides an innovative overview of designing and utilizing MBNs for oral antimicrobial applications, bridging the gap between nanomaterial design and clinical dentistry needs while guiding the development of next-generation antimicrobials in the post-antibiotic era. Firstly, we categorize and elucidate the main antibacterial mechanisms of metallic components in MBNs. Furthermore, a comprehensive summary is provided on the up-to-date advancements in using MBNs for oral antibacterial purposes, highlighting the pivotal role of metals in enhancing antibacterial properties. Finally, we discuss the existing challenges and potential future developments to establish a theoretical foundation for ongoing progress and clinical approval.</div></div>","PeriodicalId":100036,"journal":{"name":"Advanced Sensor and Energy Materials","volume":"4 2","pages":"Article 100141"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143843783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-06-01Epub Date: 2025-03-08DOI: 10.1016/j.asems.2025.100148
Jiahao Shen , Junli Liu , Yunxiao Yi , Chenhui He , Hengyu Liu , Linrong Shi , Jin Liu , Pingen Shi , Hui Liu , Xuanmeng He , Yi Feng , Xingjian Song , Shaowei Chen
Bacterial and viral infections have been a global challenge, exacerbated by rampant antibiotic overuse. It is thus of fundamental and technological significance to develop effective antibacterial agents. Herein, copper is atomically dispersed into a MoS2 matrix via the chelation of ammonium tetrathiomolybdate [(NH4)2MoS4]. Meticulous control of the copper content enables uniform atomic dispersion and optimizes active site accessibility, both critical factors for a range of catalytic activities that mimic native enzymes like peroxidase, superoxide dismutase and glutathione oxidase. Among the series, the Cu/MoS2-3 sample, with a Cu:Mo molar ratio of ca. 0.3, exhibits the best activity, with a maximum rate of 14.3 × 10−18 M s–1 in the peroxidase-like reaction with H2O2 and rate constant of 2.56 × 10−3 s−1 that are at least one order of magnitude greater than those of MoS2. These unique properties endow the resultant Cu/MoS2 composites with a remarkable antimicrobial activity. Experimentally, with the addition of 1 mM H2O2, 99% of Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli can be eliminated within 10 min by Cu/MoS2 (50 μg/mL). Such a peroxidase-like activity of Cu/MoS2 can facilitate wound healing and inflammation reduction in a Staphylococcus aureus infected wound model. Results from this study highlight the unique significance of atomic dispersion in the structural engineering of high-performance bactericidal agents for biomedical applications.
细菌和病毒感染一直是全球性的挑战，抗生素的过度使用加剧了这一挑战。因此，开发有效的抗菌药物具有重要的基础和技术意义。在这里，铜通过四硫钼酸铵[(NH4)2MoS4]的螯合作用被原子分散到MoS2基质中。对铜含量的精细控制可以实现均匀的原子分散和优化活性位点的可达性，这两个因素都是模拟天然酶（如过氧化物酶、超氧化物歧化酶和谷胱甘肽氧化酶）的催化活性的关键因素。其中Cu/MoS2-3样品表现出最好的活性，Cu:Mo摩尔比约为0.3，在与H2O2的类过氧化物酶反应中，最大速率为14.3 × 10−18 M s - 1，速率常数为2.56 × 10−3 s - 1，比MoS2的速率常数至少大一个数量级。这些独特的性质使Cu/MoS2复合材料具有显著的抗菌活性。实验表明，在加入1 mM H2O2的条件下，铜/MoS2 （50 μg/mL）在10 min内可杀灭99%的革兰氏阳性金黄色葡萄球菌和革兰氏阴性大肠杆菌。在金黄色葡萄球菌感染的伤口模型中，Cu/MoS2的过氧化物酶样活性可以促进伤口愈合和炎症减轻。这一研究结果突出了原子分散在高性能生物医学杀菌剂结构工程中的独特意义。
{"title":"Enhanced multienzyme-like and antibacterial activity by copper atomically dispersed into molybdenum disulfide for accelerated wound healing","authors":"Jiahao Shen , Junli Liu , Yunxiao Yi , Chenhui He , Hengyu Liu , Linrong Shi , Jin Liu , Pingen Shi , Hui Liu , Xuanmeng He , Yi Feng , Xingjian Song , Shaowei Chen","doi":"10.1016/j.asems.2025.100148","DOIUrl":"10.1016/j.asems.2025.100148","url":null,"abstract":"<div><div>Bacterial and viral infections have been a global challenge, exacerbated by rampant antibiotic overuse. It is thus of fundamental and technological significance to develop effective antibacterial agents. Herein, copper is atomically dispersed into a MoS<sub>2</sub> matrix via the chelation of ammonium tetrathiomolybdate [(NH<sub>4</sub>)<sub>2</sub>MoS<sub>4</sub>]. Meticulous control of the copper content enables uniform atomic dispersion and optimizes active site accessibility, both critical factors for a range of catalytic activities that mimic native enzymes like peroxidase, superoxide dismutase and glutathione oxidase. Among the series, the Cu/MoS<sub>2</sub>-3 sample, with a Cu:Mo molar ratio of ca. 0.3, exhibits the best activity, with a maximum rate of 14.3 × 10<sup>−18</sup> M s<sup>–</sup><sup>1</sup> in the peroxidase-like reaction with H<sub>2</sub>O<sub>2</sub> and rate constant of 2.56 × 10<sup>−3</sup> s<sup>−1</sup> that are at least one order of magnitude greater than those of MoS<sub>2</sub>. These unique properties endow the resultant Cu/MoS<sub>2</sub> composites with a remarkable antimicrobial activity. Experimentally, with the addition of 1 mM H<sub>2</sub>O<sub>2</sub>, 99% of Gram-positive <em>Staphylococcus aureus</em> and Gram-negative <em>Escherichia coli</em> can be eliminated within 10 min by Cu/MoS<sub>2</sub> (50 μg/mL). Such a peroxidase-like activity of Cu/MoS<sub>2</sub> can facilitate wound healing and inflammation reduction in a <em>Staphylococcus aureus</em> infected wound model. Results from this study highlight the unique significance of atomic dispersion in the structural engineering of high-performance bactericidal agents for biomedical applications.</div></div>","PeriodicalId":100036,"journal":{"name":"Advanced Sensor and Energy Materials","volume":"4 2","pages":"Article 100148"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143792342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-06-01Epub Date: 2024-10-30DOI: 10.1016/j.asems.2024.100125
Rudi Liu , Jiuxing Li , Jimmy Gu , Bruno J. Salena , Yingfu Li
The COVID-19 pandemic emphasizes the need for the development of molecular tools that can be used as effective diagnostic and therapeutic agents. Herein we investigate the potential of aptamer-dressed nanomaterials both as diagnostics and therapeutics using SARS-CoV-2 as a model. The nanomaterials are based on the palladium-iridium (Pd–Ir) nanocubes modified with monomeric, dimeric or trimeric aptamers that exhibit varying affinities for the spike protein of SARS-CoV-2. These nanomaterials were first examined for diagnostic potential through the creation of a nanozyme-linked aptamer assay (NLAA) that takes advantage of the peroxidase-mimicking activity of Pd–Ir nanocubes. The trimeric aptamer-based NLAA demonstrated a limit of detection (LOD) of 9.3×103 cp/mL for pseudoviruses expressing the spike protein of SARS-CoV-2, 172- and 12.9-fold lower than that of the monomeric and dimeric aptamer-based NLAAs, respectively. Upon testing with 60 clinical saliva samples, the trimeric aptamer-based NLAA achieved a specificity of 100% and a sensitivity of 86.7%. The same nanomaterials were also examined for the ability to block viral entry to host cells. The trimeric aptamer-conjugated nanocubes exhibited a superior neutralizing ability, with an IC50 value of 6.4 pM, 2.7-fold and 10.1-fold lower than that of the dimeric and monomeric aptamer nanocubes. Moreover, the trimeric aptamer-conjugated nanocubes exhibited excellent biostability and biocompatibility. Overall, our study provides a framework for combating future viral pandemics through the development of a paired biosensor and neutralizing agent made of the same aptamer-modified nanomaterial that recognizes an important viral surface protein like the spike protein of SARS-CoV-2.
{"title":"Palladium–iridium nanocubes modified with a high-affinity DNA aptamer as paired viral diagnostic and therapeutic tools","authors":"Rudi Liu , Jiuxing Li , Jimmy Gu , Bruno J. Salena , Yingfu Li","doi":"10.1016/j.asems.2024.100125","DOIUrl":"10.1016/j.asems.2024.100125","url":null,"abstract":"<div><div>The COVID-19 pandemic emphasizes the need for the development of molecular tools that can be used as effective diagnostic and therapeutic agents. Herein we investigate the potential of aptamer-dressed nanomaterials both as diagnostics and therapeutics using SARS-CoV-2 as a model. The nanomaterials are based on the palladium-iridium (Pd–Ir) nanocubes modified with monomeric, dimeric or trimeric aptamers that exhibit varying affinities for the spike protein of SARS-CoV-2. These nanomaterials were first examined for diagnostic potential through the creation of a nanozyme-linked aptamer assay (NLAA) that takes advantage of the peroxidase-mimicking activity of Pd–Ir nanocubes. The trimeric aptamer-based NLAA demonstrated a limit of detection (LOD) of 9.3×10<sup>3</sup> cp/mL for pseudoviruses expressing the spike protein of SARS-CoV-2, 172- and 12.9-fold lower than that of the monomeric and dimeric aptamer-based NLAAs, respectively. Upon testing with 60 clinical saliva samples, the trimeric aptamer-based NLAA achieved a specificity of 100% and a sensitivity of 86.7%. The same nanomaterials were also examined for the ability to block viral entry to host cells. The trimeric aptamer-conjugated nanocubes exhibited a superior neutralizing ability, with an IC<sub>50</sub> value of 6.4 pM, 2.7-fold and 10.1-fold lower than that of the dimeric and monomeric aptamer nanocubes. Moreover, the trimeric aptamer-conjugated nanocubes exhibited excellent biostability and biocompatibility. Overall, our study provides a framework for combating future viral pandemics through the development of a paired biosensor and neutralizing agent made of the same aptamer-modified nanomaterial that recognizes an important viral surface protein like the spike protein of SARS-CoV-2.</div></div>","PeriodicalId":100036,"journal":{"name":"Advanced Sensor and Energy Materials","volume":"4 2","pages":"Article 100125"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143705857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-06-01Epub Date: 2025-03-18DOI: 10.1016/j.asems.2025.100150
Zhong-Hui Sun , Qiu-Ling Huang , Zhan-Chao Li , Wei Zheng , Yan Mao , Dong-Xue Han , Gang Huang
The portable electrochemical sensors couple with high-energy density batteries lay the foundation for intelligent electronic devices capable of real-time and long-term monitoring of signals at the molecular level. Currently, high-entropy materials play a crucial role in advanced energy storage system and electroanalytical chemistry due to their powerful multi active centers and lattice strain fields. Herein, we propose high-entropy Prussian blue analogues (HE-PBA) as a bidirectional catalyst to reduce the activation energy of sulfur redox reaction, alleviate polysulfides shuttle, and inhibit lithium dendritic growth in Li–S battery. Furthermore, benefited from hierarchical HE-PBA with multiple redox active sites, superior ion-selective effect, high ionic/electrical conductivity and hydrophobicity, thus contributing to splendid ion-electron transducer capability as solid contact layer in wearable potentiometric electrochemical sensors. As a result, an advanced wearable electronic device integrates LSB as a power source with potentiometric electrochemical sensor unit equipped with ion selective electrode, enabling real-time monitoring of K+ concentration in sweat metabolite during outdoor exercise. In a word, this work demonstrates a tremendous potential of designing multifunctional electrode materials for advanced energy storage and electrochemical sensing applications through high entropy strategies.
{"title":"Wearable electrochemical sensor for real-time sweat monitoring powered by Li–S battery: Rapid ion-electron transduction driven by high-entropy Prussian blue analogues","authors":"Zhong-Hui Sun , Qiu-Ling Huang , Zhan-Chao Li , Wei Zheng , Yan Mao , Dong-Xue Han , Gang Huang","doi":"10.1016/j.asems.2025.100150","DOIUrl":"10.1016/j.asems.2025.100150","url":null,"abstract":"<div><div>The portable electrochemical sensors couple with high-energy density batteries lay the foundation for intelligent electronic devices capable of real-time and long-term monitoring of signals at the molecular level. Currently, high-entropy materials play a crucial role in advanced energy storage system and electroanalytical chemistry due to their powerful multi active centers and lattice strain fields. Herein, we propose high-entropy Prussian blue analogues (HE-PBA) as a bidirectional catalyst to reduce the activation energy of sulfur redox reaction, alleviate polysulfides shuttle, and inhibit lithium dendritic growth in Li–S battery. Furthermore, benefited from hierarchical HE-PBA with multiple redox active sites, superior ion-selective effect, high ionic/electrical conductivity and hydrophobicity, thus contributing to splendid ion-electron transducer capability as solid contact layer in wearable potentiometric electrochemical sensors. As a result, an advanced wearable electronic device integrates LSB as a power source with potentiometric electrochemical sensor unit equipped with ion selective electrode, enabling real-time monitoring of K<sup>+</sup> concentration in sweat metabolite during outdoor exercise. In a word, this work demonstrates a tremendous potential of designing multifunctional electrode materials for advanced energy storage and electrochemical sensing applications through high entropy strategies.</div></div>","PeriodicalId":100036,"journal":{"name":"Advanced Sensor and Energy Materials","volume":"4 2","pages":"Article 100150"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143828520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-06-01Epub Date: 2025-03-10DOI: 10.1016/j.asems.2024.100130
Huang Dai , Huilin Hu , Zhiyong Gong , Jing Shu , Jiahua Wang , Xiaodan Liu , Fuwei Pi , Qiao Wang , Shuo Duan , Yingli Wang
{"title":"Corrigendum to “Needle−tip effect promoted flexible electrochemical sensor for detecting chloride ions in food by in−situ deposited silver dendrimers” [Adv Sensor Energy Mater 3 (2024) 100100]","authors":"Huang Dai , Huilin Hu , Zhiyong Gong , Jing Shu , Jiahua Wang , Xiaodan Liu , Fuwei Pi , Qiao Wang , Shuo Duan , Yingli Wang","doi":"10.1016/j.asems.2024.100130","DOIUrl":"10.1016/j.asems.2024.100130","url":null,"abstract":"","PeriodicalId":100036,"journal":{"name":"Advanced Sensor and Energy Materials","volume":"4 2","pages":"Article 100130"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143580021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-06-01Epub Date: 2024-12-10DOI: 10.1016/j.asems.2024.100131
Qian Chen , Jie Su , Xiaojun Bian, Hongmin Zhang, Shiqi Yang, Juan Yan
The detection and cultivation of circulating tumor cells (CTCs) play a crucial role in monitoring tumor recurrence, metastasis, early disease diagnosis, and assessing the effectiveness of drug treatments. This study specifically focused on investigating human breast cancer cells MCF-7 by utilizing framework nucleic acids (FNAs) as bio-probe scaffold in conjunction with fishbone structures and three-dimensional (3D) microcavity structures within microchannels. These components collectively formed an integrated nano-micro interface system designed for a comprehensive examination of CTC detection and cell culture. The study involved the assessment and comparison of rigid 3D FNAs with distinct side lengths of 7, 13, and 26 bases. This approach not only allowed for precise regulation of the DNA biosensor interface through the manipulation of probe spacing, facilitating optimal probe-cell interactions within the microfluidic channel. Consequently, this approach significantly enhances capture efficiency and lowers the CTC detection limit to 5 cells/mL. Moreover, this research successfully observed cell proliferation and individual cell biological behavior within the 3D microcavity structure. The findings indicated that the overall cell population's proliferation was like that in static culture conditions. Although the proliferation cycle of individual cells was notably extended, cell mobility within the microcavity demonstrated their robust biological activity. These significant outcomes not only offer a practical approach for early tumor detection but also provide a valuable pathway for comprehending mechanisms of tumor development and advancement and guiding personalized treatment strategies effectively.
{"title":"Utilizing framework nucleic acids for integrated nano-micro interface system in circulating tumor cells (CTCs) detection, cultivation, and single-cell analysis","authors":"Qian Chen , Jie Su , Xiaojun Bian, Hongmin Zhang, Shiqi Yang, Juan Yan","doi":"10.1016/j.asems.2024.100131","DOIUrl":"10.1016/j.asems.2024.100131","url":null,"abstract":"<div><div>The detection and cultivation of circulating tumor cells (CTCs) play a crucial role in monitoring tumor recurrence, metastasis, early disease diagnosis, and assessing the effectiveness of drug treatments. This study specifically focused on investigating human breast cancer cells MCF-7 by utilizing framework nucleic acids (FNAs) as bio-probe scaffold in conjunction with fishbone structures and three-dimensional (3D) microcavity structures within microchannels. These components collectively formed an integrated nano-micro interface system designed for a comprehensive examination of CTC detection and cell culture. The study involved the assessment and comparison of rigid 3D FNAs with distinct side lengths of 7, 13, and 26 bases. This approach not only allowed for precise regulation of the DNA biosensor interface through the manipulation of probe spacing, facilitating optimal probe-cell interactions within the microfluidic channel. Consequently, this approach significantly enhances capture efficiency and lowers the CTC detection limit to 5 cells/mL. Moreover, this research successfully observed cell proliferation and individual cell biological behavior within the 3D microcavity structure. The findings indicated that the overall cell population's proliferation was like that in static culture conditions. Although the proliferation cycle of individual cells was notably extended, cell mobility within the microcavity demonstrated their robust biological activity. These significant outcomes not only offer a practical approach for early tumor detection but also provide a valuable pathway for comprehending mechanisms of tumor development and advancement and guiding personalized treatment strategies effectively.</div></div>","PeriodicalId":100036,"journal":{"name":"Advanced Sensor and Energy Materials","volume":"4 2","pages":"Article 100131"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143600910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-06-01Epub Date: 2025-02-25DOI: 10.1016/j.asems.2025.100138
Lihua Wang
{"title":"Editorial: DNA and inorganic nanomaterials for sensors and energy","authors":"Lihua Wang","doi":"10.1016/j.asems.2025.100138","DOIUrl":"10.1016/j.asems.2025.100138","url":null,"abstract":"","PeriodicalId":100036,"journal":{"name":"Advanced Sensor and Energy Materials","volume":"4 2","pages":"Article 100138"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143835010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-06-01Epub Date: 2025-02-28DOI: 10.1016/j.asems.2025.100142
Sharma T. Sanjay , Sapna Kannan , XiuJun Li
Novel strategies for the simultaneous and portable detection of multiple analytes are highly favorable for clinical diagnosis and healthcare. Conventional colorimetric enzyme-linked immunosorbent assay (ELISA) is a widely used laboratory technique for medical diagnostics, quality control, and research applications. However, nonspecific absorption of proteins may lead to a reduction of functional sites, resulting in high background and low sensitivity in ELISA. Herein, we report a simple method of functionalization of poly(methyl methacrylate) (PMMA) with polylysine to be used as the microfluidic microplate substrate for enhanced ELISA, enabling rapid, ultrasensitive, and multiplexed detection of infectious diseases. FTIR and fluorescence microscopy characterization confirmed high amine densities on polylysine-modified PMMA surface, resulting in high detection sensitivity of the colorimetric ELISA on the PMMA microdevice. The ultrasensitive polylysine-modified microplate can immobilize protein within 20 min and results of the assay can be viewed by the naked eye or scanned through a simple desktop scanner for quantitative analysis within 90 min. A sandwich-type immunoassay for the rapid and sensitive detection of immunoglobulin G (IgG), hepatitis B surface antigen (HBsAg), and hepatitis B core antigen (HBcAg) was demonstrated as a proof-of-concept for multiplexed detection. The limits of detection (LOD) of 200.0 pg/mL for IgG, 180.0 pg/mL for HBsAg, and 300.0 pg/mL for HBcAg were achieved, without any specialized equipment like a microplate reader. The surface-modified microchip exhibited about 10-fold higher sensitivity than traditional microplates. This surface-modified microplate has tremendous potential as a point-of-care multiplexed testing platform for many applications ranging from clinical diagnosis to environmental monitoring, particularly in resource-limited settings.
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