Advances in Sample Preparation最新文献_第9页

Development of an automated sorptive extraction system for trace analysis in water 用于水中痕量分析的自动吸附萃取系统的研制

IF 5.2 Q1 CHEMISTRY, ANALYTICAL

Advances in Sample Preparation

Pub Date : 2025-02-01 DOI: 10.1016/j.sampre.2025.100156

Frank Jacobs , Dr. Klaus Kerpen , Dr. Eike Kleine-Benne , PD Dr. Ursula Telgheder

Stir bar sorptive extraction (SBSE) is an established microextraction technique developed in the early 2000s. It has gained widespread use in laboratories around the world, but always had one drawback in that it was a manual sample preparation technique. The newly developed system presented in this study aims to combine the well-known characteristics of SBSE with fully automated sample preparation. The presented system is based on a novel sample probe design, which uses the same dimensions and materials of sorbent phase as the commercially available Gerstel Twister® SBSE technology. To test the performance of this system, pesticides were extracted from pure water as well as river water. Limits of detection (4.5 ng/L to 53.6 ng/L) and quantification (15.7 ng/L to 327.6 ng/L) as well as standard method deviation (3.2 % to 37.6 %) were determined for 21 pesticides based on DIN EN ISO 27,108, part of the water framework directive (WFR). Standard addition was used to quantify pesticides in river water matrix. To check the performance of the new development, it was compared to established methods like manual SBSE, thin film micro extraction (TFME) and SPME-Arrow. The results are comparable between automated sorptive extraction (ASE) and SBSE (average of 84 % compared to automated system), as expected. Performance compared to TFME (65 %) and SPME-Arrow (3 %) was significantly better, especially to the latter. These results show that the automated sorptive extraction system developed in this paper is competitive with already established techniques.

搅拌棒吸附萃取（SBSE）是21世纪初发展起来的一种成熟的微萃取技术。它在世界各地的实验室中得到了广泛的应用，但一直有一个缺点，那就是它是一种手工样品制备技术。本研究中提出的新开发的系统旨在将SBSE众所周知的特性与全自动样品制备相结合。提出的系统是基于一种新的样品探针设计，它使用相同的尺寸和材料的吸附剂相作为市售的Gerstel Twister®SBSE技术。为了测试该系统的性能，分别从纯净水和河水中提取农药。根据水框架指令（WFR）的一部分DIN EN ISO 27,108，确定了21种农药的检出限（4.5 ng/L ~ 53.6 ng/L）、定量限（15.7 ng/L ~ 327.6 ng/L）和标准方法偏差（3.2% ~ 37.6%）。采用标准添加法对河流水基质中农药进行定量分析。为了检查新开发的性能，将其与现有的方法（如手动SBSE，薄膜微萃取（TFME）和SPME-Arrow）进行比较。结果与预期的一样，自动吸附萃取（ASE）和SBSE（与自动化系统相比平均为84%）之间具有可比性。与TFME（65%）和SPME-Arrow（3%）相比，性能明显更好，尤其是后者。这些结果表明，本文开发的自动吸附萃取系统与已有的技术相比具有竞争力。

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引用次数: 0

Vial-coated thin film microextraction for perfluoroalkyl carboxylic acids (PFCAs) determination in water by GC–MS 气相色谱-质谱法测定水中全氟烷基羧酸（PFCAs）

IF 5.2 Q1 CHEMISTRY, ANALYTICAL

Advances in Sample Preparation

Pub Date : 2025-02-01 DOI: 10.1016/j.sampre.2025.100147

J․Mabel Luna-Díaz , Luz O․ Leal-Quezada , Laura Ferrer , Edwin Palacio

Perfluoroalkyl carboxylic acids (PFCAs), a subclass of perfluoroalkyl substances (PFAs) commonly detected in water, are of increasing concern due to their persistence, bioaccumulative nature, and harmful effects on health. Determining PFCAs in environmental matrices is challenging because they are typically present at ng L⁻¹ levels and tend to adsorb onto materials like filtration membranes, causing significant losses during analysis. To address these challenges, a thin-film microextraction (TFME)-coated vial was designed for PFCA (C5–C10) extraction, coupled with gas chromatography-mass spectrometry (GC–MS). Glass was selected as the support material due to its low adsorption rate and superior chemical resistance compared to other polymers. The TFME coating, made from a weak anion exchange resin and polyacrylonitrile (10 % w/w) as a binder polymer, effectively extracts analytes from complex matrices while minimizing co-extraction of interfering macromolecules. The method eliminates the need for sample filtration, preventing PFCA loss in filtration membranes. Under optimal conditions, the extraction efficiency ranged from 70 to 100 %. Detection and quantification limits were 0.13–2.1 ng L⁻¹ and 0.42–7.1 ng L⁻¹, respectively. Intra- and inter-assay relative standard deviations (% RSD) were 4.9–2.2 % and 4.8–2.3 %, respectively, with a linear working range of 10–1000 ng L⁻¹ for the TFME-coated device. This method is suitable for PFCA determination in drinking water and complies with the Directive 2020/2184, which sets a maximum allowable limit of 500 ng L⁻¹ for total PFAs.

全氟烷基羧酸（PFCAs）是常见于水中的全氟烷基物质（PFAs）的一个亚类，由于其持久性、生物蓄积性和对健康的有害影响而日益受到关注。测定环境基质中的PFCAs具有挑战性，因为它们通常以ng L -¹的水平存在，并且倾向于吸附在过滤膜等材料上，在分析过程中造成重大损失。为了解决这些挑战，设计了一种薄膜微萃取（TFME）包被的小瓶，用于PFCA （C5-C10）的提取，并结合气相色谱-质谱（GC-MS）。与其他聚合物相比，玻璃的吸附率低，耐化学性好，因此选择玻璃作为支撑材料。TFME涂层由弱阴离子交换树脂和聚丙烯腈（10% w/w）作为粘合剂聚合物制成，可以有效地从复杂基质中提取分析物，同时最大限度地减少干扰大分子的共萃取。该方法消除了样品过滤的需要，防止了PFCA在过滤膜中的损失。在最佳条件下，提取效率为70% ~ 100%。检测限和定量限分别为0.13-2.1 ng L⁻¹和0.42-7.1 ng L⁻¹。测定内和测定间的相对标准偏差（% RSD）分别为4.9 - 2.2%和4.8 - 2.3%，线性工作范围为10-1000 ng L - 1。本方法适用于饮用水中PFCA的测定，符合指令2020/2184，该指令规定了PFAs总量的最大允许限量为500 ng L - 1。

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引用次数: 0

Towards eco-metabolomics: NADES-guided extraction enables semi-quantitative metabolomics for Melissa officinalis 走向生态代谢组学：nades引导提取实现了药用草的半定量代谢组学

IF 5.2 Q1 CHEMISTRY, ANALYTICAL

Advances in Sample Preparation

Pub Date : 2025-02-01 DOI: 10.1016/j.sampre.2025.100154

Chiara Spaggiari , Isa Sara Aimee Hiemstra , Antoinette Kazbar , Gabriele Costantino , Laura Righetti

In recent years, there has been a growing emphasis on the development of green extraction techniques that minimize environmental impact while maximizing yield of the extracted compounds. To this aim, in this study we investigated the potential of green solvents for extracting bioactive compounds from Melissa officinalis (MO) leaves. Specifically, we focus on the application of 20 Natural Deep Eutectic Solvents (NADES) with a relative polarity ranging from 0.34 to 1.29. Their extraction affinity against a set of 11 plant metabolites was predicted using COSMO-RS software and experimentally validated using quantitative LCHRMS analysis. Subsequently, the same extracts were subjected to non-target metabolomics to uncover the NADES selectivity towards the wide spectrum of MO leaf metabolites. Data preprocessing and feature alignment were performed using MZmine, and aligned features were annotated using SIRIUS+CSI:FingerID.

Overall, 249 and 195, metabolites were annotated in positive and negative ionization ion mode, respectively. Additionally, to have a more accurate view of the different NADES extraction capacity, we adopted a semi-quantitative approach that enables the prediction of concentration for all the annotated metabolites (N = 444).

The results highlighted the selectivity of some NADES in extracting very diverse biochemical classes, providing valuable insights into the composition and concentration of bioactive compounds. Interestingly, thymol-menthol NADES demonstrated the ability to efficiently extract a broad range of bioactive compounds, yielding a metabolome comparable to that obtained with conventional ethanolic. Overall, the entire workflow facilitated the green extraction and annotation of known bioactive molecules that had never been described in MO.

近年来，人们越来越重视绿色提取技术的发展，以尽量减少对环境的影响，同时最大限度地提高提取化合物的产量。为此，本研究探讨了绿色溶剂提取梅丽莎叶中生物活性物质的潜力。具体来说，我们重点研究了20种相对极性在0.34 ~ 1.29之间的天然深共晶溶剂（NADES）的应用。利用cosmoo - rs软件预测了它们对11种植物代谢物的提取亲和力，并通过定量LCHRMS分析进行了实验验证。随后，对相同的提取物进行非靶代谢组学研究，以揭示NADES对MO叶代谢物的广谱选择性。使用MZmine进行数据预处理和特征对齐，对齐后的特征使用SIRIUS+CSI:FingerID进行标注。总的来说，249和195种代谢物分别以正离子和负离子模式标注。此外，为了更准确地了解不同的NADES提取能力，我们采用了半定量方法，可以预测所有注释代谢物的浓度（N = 444）。结果突出了一些NADES在提取非常不同的生化类中的选择性，为生物活性化合物的组成和浓度提供了有价值的见解。有趣的是，百里香薄荷醇NADES能够有效地提取广泛的生物活性化合物，产生与传统乙醇相当的代谢组。总的来说，整个工作流程促进了MO中从未描述过的已知生物活性分子的绿色提取和注释。

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引用次数: 0

Electrospun chitosan modified montmorillonite/polyvinyl alcohol nanofibers as suitable coating for fiber-in-tube solid-phase microextraction of trace amounts of tricyclic antidepressants in biological fluids 静电纺壳聚糖改性蒙脱土/聚乙烯醇纳米纤维涂层用于管中纤维固相微萃取生物体液中微量三环类抗抑郁药

IF 5.2 Q1 CHEMISTRY, ANALYTICAL

Advances in Sample Preparation

Pub Date : 2025-02-01 DOI: 10.1016/j.sampre.2024.100145

Saeed Pourhosseini, Yadollah Yamini, Seyedeh Sara Nasrollahi

Chitosan intercalated montmorillonite and polyvinyl alcohol-citric acid (PVA/CA) nanofibers were electrospun onto stainless steel wires. The fibers were placed inside a stainless steel tube and utilized for fiber-in-tube solid phase microextraction (FIT-SPME) to detect trace levels of tricyclic antidepressant drugs (TCAs) in biological samples. The analytes were separated and quantified using gas chromatography coupled with mass spectrometric detection (GC–MS). The effects of various parameters on the extraction efficiency, including pH (9), extraction time (25 min), extraction flow rate (3 mL min^-1), salt addition (0 % w/v), type of eluent (ethanol 99 %), eluent volume (300 µL), and desorption flow rate (70 µL min^-1), were investigated and optimized. Under optimal conditions, the linear dynamic range for doxepin, and sertraline was found to be 0.08–750.0 ng mL^-1; for amitriptyline, desipramine, and trimipramine, it was 10.0–750.0 ng mL^-1; and for carbamazepine and maprotiline, it was 50.0–750.0 ng mL^-1. The proposed method exhibited low limits of detections in the range of 0.02–15.2 ng mL^-1. Additionally, coefficients of determination (R²) exceeding 0.9911 were achieved, indicating excellent linearity for the calibration curves. To assess the feasibility of the FIT-SPME-GC–MS method, extraction and analysis of TCAs were performed on plasma and urine samples. Relative recovery percentages (RR%) ranged from 90.3 % to 117.6 %.

将壳聚糖嵌层蒙脱土和聚乙烯醇-柠檬酸（PVA/CA）纳米纤维电纺成不锈钢丝。这些纤维被放置在一个不锈钢管中，用于纤维管固相微萃取（FIT-SPME），以检测生物样品中痕量的三环抗抑郁药物（TCAs）。采用气相色谱-质谱联用检测（GC-MS）对分析物进行分离和定量。考察了pH(9)、提取时间（25 min）、提取流量（3 mL min-1）、盐添加量（0% w/v）、洗脱液类型（99%乙醇）、洗脱液体积（300µL）、解吸流量（70µL min-1）等参数对提取效率的影响，并进行了优化。在最佳条件下，多塞平和舍曲林的线性动态范围为0.08 ~ 750.0 ng mL-1；阿米替林、地西帕明和三嗪帕明为10.0 ~ 750.0 ng mL-1；卡马西平和马普替林为50.0 ~ 750.0 ng mL-1。该方法的低检出限为0.02 ~ 15.2 ng mL-1。测定系数（R²）均大于0.9911，表明标定曲线线性良好。为了评估FIT-SPME-GC-MS方法的可行性，对血浆和尿液样本进行了TCAs的提取和分析。相对回收率（RR%）为90.3% ~ 117.6%。

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引用次数: 0

Biodiesel as a green alternative solvent in dispersive liquid-liquid microextraction 生物柴油作为分散液-液微萃取的绿色替代溶剂

IF 5.2 Q1 CHEMISTRY, ANALYTICAL

Advances in Sample Preparation

Pub Date : 2025-02-01 DOI: 10.1016/j.sampre.2025.100163

Samara Soares, Fábio R.P. Rocha

Biodiesel, a fuel derived from renewable sources, is a nonpolar liquid, composed of alkyl esters derived from long-chain carboxylic acids. Because of its environmental friendliness, large-scale production, and cost-effectiveness, biodiesel is herein proposed as a green solvent for liquid-liquid microextraction. Its potential has been demonstrated through the extraction of model analytes, with varying polarities through analytical measurements performed by smartphone-based digital images. Partition coefficients biodiesel/water (log P) were –0.45 (pH 2.0) for crystal violet, –1.3 (pH 7.0) for bromocresol green, 1.05 for PAN, 3.48 for Cu(II)-PAN complex, and 2.14 for quercetin. Biodiesel was effective in extracting nonpolar species, with extraction efficiencies of 90 % for PAN, 99 % for quercetin and Cu(II)-PAN complex. Efficiency of extraction of the Cu(II)-PAN complex increased in the following order: chloroform, ethyl acetate, and biodiesel, and the highest recoveries were achieved with biodiesel as solvent. Only slightly variations in the extraction efficiency was observed from biodiesel produced from different feedstock. Biodiesel was also efficient for extraction of the ion-pairs from anionic surfactants and methylene blue, with the perspective of replacing chloroform in this classical method. The results showed that biodiesel is an efficient alternative solvent to replace typical toxic solvents, with greenness demonstrated through the Green Star and AGREE metrics.

生物柴油是一种由长链羧酸衍生的烷基酯组成的非极性液体，是一种可再生能源燃料。由于生物柴油具有环境友好、可大规模生产和成本效益高的特点，本文提出生物柴油作为液液微萃取的绿色溶剂。它的潜力已经通过模型分析物的提取得到证明，通过基于智能手机的数字图像进行分析测量，具有不同的极性。结晶紫的生物柴油/水分配系数（log P）为-0.45 (pH 2.0)，溴甲酚绿为-1.3 (pH 7.0)， PAN为1.05,Cu(II)-PAN配合物为3.48，槲皮素为2.14。生物柴油可有效提取非极性物质，对PAN的提取率为90%，对槲皮素和Cu(II)-PAN配合物的提取率为99%。铜(II)-PAN络合物的萃取效率依次为氯仿、乙酸乙酯、生物柴油，以生物柴油为溶剂的萃取回收率最高。从不同原料生产的生物柴油中，提取效率只有轻微的变化。生物柴油对阴离子表面活性剂和亚甲基蓝中离子对的提取也很有效，有望取代氯仿。结果表明，生物柴油是一种有效的替代溶剂，可以替代典型的有毒溶剂，并通过绿色之星和AGREE指标证明其绿色。

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引用次数: 0

Tailoring a MOF-on-MOF (ZIF-8-on-NH2-MIL-53(Al)) Hybrid Composite for Micro Solid Phase Extraction of Furosemide in artificial biological fluids prior to LC-q-TOF/MS detection 定制MOF-on-MOF （ZIF-8-on-NH2-MIL-53(Al)）混合复合材料，用于LC-q-TOF/MS检测前人工生物液中速尿的微固相萃取

IF 5.2 Q1 CHEMISTRY, ANALYTICAL

Advances in Sample Preparation

Pub Date : 2025-02-01 DOI: 10.1016/j.sampre.2025.100157

Zinar Pinar Gumus , Mustafa Soylak

Furosemide, a powerful diuretic that increases urine excretion, is used to treat many diseases. However, due to its abuse in sport, it is considered a doping substance. As a result, it has been banned by the World Anti-Doping Agency (WADA) and included in the group of diuretics and masking agents. Given the clinical significance of furosemide and its dependable application in athletics, this research employed the "MOF-on-MOF" hybrid material composite to leverage the synergistic effects of various components for detecting this drug in biological samples. After characterization of the ZIF-8-on-NH₂-MIL-53(Al) composite by FT-IR, XRD, TGA, XPS, BET and SEM-EDX techniques, the µ-SPE procedure was optimized using liquid chromatography-time of flight mass spectrometry (LC-q-TOF/MS) based on the recovery values. The optimum conditions were determined as pH 6, adsorbent amount of 20 mg, and total adsorption-desorption time of 7 min. The recovery values of the solid-phase extraction method for simulated body fluids samples under optimum conditions were between 77.5 % and 93.4 %. The µ-SPE technique was effectively utilized on physiological samples using the ZIF-8-on-NH₂-MIL-53(Al) composite under optimal conditions. This study's findings will inform future research into the design of 'MOF-on-MOF' hybrids for various applications.

速尿是一种强大的利尿剂，可以增加尿液的排出，被用来治疗许多疾病。然而，由于其在体育运动中的滥用，它被认为是一种兴奋剂物质。因此，它已被世界反兴奋剂机构（WADA）禁用，并被列入利尿剂和掩蔽剂组。鉴于呋塞米的临床意义及其在体育运动中的可靠应用，本研究采用“MOF-on-MOF”混合材料复合材料，利用各组分的协同作用在生物样品中检测呋塞米。利用FT-IR、XRD、TGA、XPS、BET和SEM-EDX等技术对ZIF-8-on-NH2-MIL-53（Al）复合材料进行表征后，利用液相色谱-飞行时间质谱（LC-q-TOF/MS）对其回收率进行优化。最佳条件为pH 6、吸附剂用量20 mg、总吸附-解吸时间7 min。在最佳条件下，固相萃取法对模拟体液样品的回收率为77.5% ~ 93.4%。在最佳条件下，利用ZIF-8-on-NH2-MIL-53（Al）复合材料对生理样品进行了微固相萃取。这项研究的发现将为未来设计各种应用的“MOF-on-MOF”混合材料的研究提供信息。

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引用次数: 0

Endogenous HiBiT-tagging combined with affinity complementation: A new strategy for small open reading frame-encoded polypeptide detection in bacteria 内源性hibit标记结合亲和互补：细菌小片段开放阅读框编码多肽检测的新策略

IF 5.2 Q1 CHEMISTRY, ANALYTICAL

Advances in Sample Preparation

Pub Date : 2025-02-01 DOI: 10.1016/j.sampre.2024.100143

Laure Simoens, Veronique Jonckheere, Dominiek Catteeuw, Petra Van Damme

Bacterial genome annotations are continuously refined with the advent of novel techniques. Ribosome profiling, or Ribo-seq, utilizing next-generation sequencing to link genomic regions with translational activity, has uncovered numerous small open reading frames (sORFs) - arbitrarily defined as ORFs no longer than 300 base pairs - as a generally under-annotated class of genomic elements in both eukaryotic and prokaryotic genomes. While sORFs can function as regulatory elements, they may also translate into small proteins (equal to or shorter than 100 amino acids), classified as sORF-encoded polypeptides (SEPs). The inherent limitations of ribosome profiling necessitate the experimental validation of predicted (s)ORFs at the protein expression level. However, the small size and unique biochemical characteristics of SEPs pose significant challenges for traditional protein detection methods, like mass spectrometry and immunoblotting. In this study, we introduce HiBiT blotting, a luminescent, complementation-based protein detection method, as a highly sensitive alternative to antibody-based immunoblotting for investigating SEP expression in Salmonella enterica (serovar) Typhimurium (S. Typhimurium) at endogenous levels. We demonstrate its complementarity to mass spectrometry as an expression validation tool. Additionally, employing a biochemical fractionation approach, we determined the subcellular localization of validated S. Typhimurium SEPs, initiating exploration into the functional aspects of these SEPs, and proposed enrichment strategies that may facilitate future experimental validation of Ribo-seq-predicted sORFs.

随着新技术的出现，细菌基因组注释不断得到完善。核糖体分析，或核糖序列，利用下一代测序将基因组区域与翻译活性联系起来，已经发现了许多小的开放阅读框架(sorf) -任意定义为不超过300个碱基对-作为真核和原核基因组中普遍未加注释的基因组元件。虽然sorf可以作为调控元件，但它们也可以翻译成小蛋白质（等于或短于100个氨基酸），被归类为sorf编码的多肽（sep）。核糖体谱分析的固有局限性需要在蛋白质表达水平上对预测的orf进行实验验证。然而，SEPs的体积小，生化特性独特，这对传统的蛋白质检测方法，如质谱法和免疫印迹法提出了重大挑战。在这项研究中，我们引入了HiBiT印迹法，一种发光的、基于互补的蛋白检测方法，作为一种高度敏感的替代基于抗体的免疫印迹法，用于研究内源性沙门氏菌（血清型）鼠伤寒沙门氏菌（S. Typhimurium）中SEP的表达。我们证明了它与质谱作为表达验证工具的互补性。此外，采用生化分离方法，我们确定了验证的鼠伤寒沙门氏菌sep的亚细胞定位，开始探索这些sep的功能方面，并提出了富集策略，可能有助于未来实验验证核糖核酸-seq预测的sorf。

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引用次数: 0

Mixed matrix membranes based on cellulose acetate recycled from cigarette butts and metal-organic frameworks for thin film solid-phase microextraction: Determination of phenols in environmental waters 基于从烟头回收的醋酸纤维素和金属有机骨架的混合基质薄膜固相微萃取：环境水中酚类物质的测定

IF 5.2 Q1 CHEMISTRY, ANALYTICAL

Advances in Sample Preparation

Pub Date : 2025-02-01 DOI: 10.1016/j.sampre.2025.100150

Massimo G. De Cesaris , María J. Trujillo-Rodríguez , Jorge Pasán , Alessandra Gentili , Verónica Pino

The recovery of raw materials from waste is a viable strategy to transition to more circular and greener methodologies. In this study, cigarette filters were recycled to obtain cellulose acetate and re-used as a support for a sorbent material in a microextraction procedure. Thus, this biosorbent was combined with a metal-organic framework (MIL-101(Fe)) and configurated in a membrane format of 300 µm of thickness via solvent casting. The developed mixed matrix membranes were characterized by Fourier-transform infrared spectroscopy, powder X-ray diffraction, scanning electron microscopy, and energy dispersion X-ray. Kinetic adsorption studies for a group of 14 phenols (chlorophenols, alkylphenols, and bisphenols) present in waters and partitioning to the membrane systems were performed to gain insights into the extraction mechanisms of the membranes. The kinetics were fitted to pseudo-first order and interparticle diffusion models, with data indicating that chloro‑ and alkyl-phenols were adsorbed faster than bisphenols. Besides, the resulting membranes were utilized in thin film solid-phase microextraction (TF-SPME) in combination with high-performance liquid chromatography (HPLC) with diode array detection (DAD) and fluorescent detector (FLD). The optimized method using these mixed matrix membranes (with dish shape and 1 cm of diameter) required 60 min of extraction with agitation followed by 10 min of desorption in 500 µL of basic ethanol. Low limits of detection, down to 2.13 µg·L^-1, were achieved, together with good performance in SPMS and BAGI metrics, with scores of 5.89 and 62.5, respectively. The performance with river waters was accompanied by adequate relative recoveries (74.3–117 %), reproducibility (with inter-day RSD values lower than 18 %), and absolute recoveries (up to 59 %).

从废物中回收原材料是向更循环和更环保的方法过渡的可行策略。在本研究中，回收香烟过滤嘴以获得醋酸纤维素，并在微萃取过程中作为吸附剂材料的载体。因此，该生物吸附剂与金属有机框架（MIL-101(Fe)）结合，并通过溶剂铸造形成300微米厚度的膜形式。采用傅里叶变换红外光谱、粉末x射线衍射、扫描电镜和能量色散x射线对制备的混合基质膜进行了表征。对存在于水中的14种酚类（氯酚、烷基酚和双酚）进行了动力学吸附研究，并对膜系统进行了分配，以深入了解膜的提取机制。动力学符合准一级和颗粒间扩散模型，数据表明氯酚和烷基酚的吸附速度比双酚快。并将所得膜结合高效液相色谱（HPLC）二极管阵列检测（DAD）和荧光检测器（FLD）用于薄膜固相微萃取（TF-SPME）。优化后的方法使用这些混合基质膜（碟形，直径1 cm），搅拌提取60 min，然后在500µL碱性乙醇中解吸10 min。检测限低至2.13µg·L-1，同时在SPMS和BAGI指标中表现良好，得分分别为5.89和62.5。该方法在河流水体中的相对回收率为74.3% ~ 117%，重现性（日间RSD值低于18%），绝对回收率为59%。

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引用次数: 0

IF 6.5 Q1 CHEMISTRY, ANALYTICAL

Advances in Sample Preparation

Pub Date : 2025-01-01

引用次数: 0

IF 6.5 Q1 CHEMISTRY, ANALYTICAL

Advances in Sample Preparation

Pub Date : 2025-01-01

引用次数: 0