Pub Date : 2021-01-29DOI: 10.37895/2071-8004-2020-24-2-3-78-84
V. V. Masljakov, T. Allahjarov, S. Kulikov, M. A. Shihmagomedov
Actuality . Surgical interventions in case of spleen injury are aimed to achieve a reliable hemostasis, but due to the morphological structure of the spleen suturing does not lead to this goal; therefore, in most cases, surgeries end with splenectomy. However, removal of the spleen causes postplenectomic hyposplenism, and it urges to search for other modalities alternative to splenectomy. Purpose . To assess the effectiveness of interventions with minimal access in the left hypochondrium for organ-sparing surgery (OSS) on the spleen with laser light application. Material and methods . Outcomes of surgical treatment in 126 patients with closed spleen injury were analyzed. Of the total number of patients, organ-preserving surgeries with laser light were performed in 59 (46.8%) cases; among them 36 (28.6%) patients were operated on with minimal access surgery, and in case of upper midline laparotomy – in 23 (18.2%) cases. Medilas fibertom 5100 laser device (DORNIER Ltd) with wavelength 1064 nm was used for the discussed organ-sparing surgeries. In all cases, surgeries were ended with drainage of the abdominal cavity. Results . As it has been found out, the most optimal technique for OSS in spleen injury is laparoscopy which allows not only to evaluate the volume of intra-abdominal blood loss, but also to define the nature of spleen injury and presence or absence of damage to other abdominal organs. The minimal approach for closed spleen injury has both advantages and disadvantages. The advantages are technical convenience in performing surgery on the spleen and possibility to apply laser technology for OSS. However, OSS with laser light on the spleen with closed injury is recommended if patient’s hemodynamics is stable, organ injury is isolated, OSS is technically possible and there is no any damage to vessels of the spleen hilus. A significant disadvantage of minimal access surgeries is inability to perform a thorough revision of abdominal organs what is compulsory in urgent cases. Conclusion . Minimal access surgery with high-level laser energy in closed spleen injury is possible only if spleen injury is isolated.
{"title":"Minimal access surgery for organ-sparing interventions with laser light in closed spleen injury: Advantages and disadvantages","authors":"V. V. Masljakov, T. Allahjarov, S. Kulikov, M. A. Shihmagomedov","doi":"10.37895/2071-8004-2020-24-2-3-78-84","DOIUrl":"https://doi.org/10.37895/2071-8004-2020-24-2-3-78-84","url":null,"abstract":"Actuality . Surgical interventions in case of spleen injury are aimed to achieve a reliable hemostasis, but due to the morphological structure of the spleen suturing does not lead to this goal; therefore, in most cases, surgeries end with splenectomy. However, removal of the spleen causes postplenectomic hyposplenism, and it urges to search for other modalities alternative to splenectomy. Purpose . To assess the effectiveness of interventions with minimal access in the left hypochondrium for organ-sparing surgery (OSS) on the spleen with laser light application. Material and methods . Outcomes of surgical treatment in 126 patients with closed spleen injury were analyzed. Of the total number of patients, organ-preserving surgeries with laser light were performed in 59 (46.8%) cases; among them 36 (28.6%) patients were operated on with minimal access surgery, and in case of upper midline laparotomy – in 23 (18.2%) cases. Medilas fibertom 5100 laser device (DORNIER Ltd) with wavelength 1064 nm was used for the discussed organ-sparing surgeries. In all cases, surgeries were ended with drainage of the abdominal cavity. Results . As it has been found out, the most optimal technique for OSS in spleen injury is laparoscopy which allows not only to evaluate the volume of intra-abdominal blood loss, but also to define the nature of spleen injury and presence or absence of damage to other abdominal organs. The minimal approach for closed spleen injury has both advantages and disadvantages. The advantages are technical convenience in performing surgery on the spleen and possibility to apply laser technology for OSS. However, OSS with laser light on the spleen with closed injury is recommended if patient’s hemodynamics is stable, organ injury is isolated, OSS is technically possible and there is no any damage to vessels of the spleen hilus. A significant disadvantage of minimal access surgeries is inability to perform a thorough revision of abdominal organs what is compulsory in urgent cases. Conclusion . Minimal access surgery with high-level laser energy in closed spleen injury is possible only if spleen injury is isolated.","PeriodicalId":10008,"journal":{"name":"Chinese Journal of Laser Medicine & Surgery","volume":"74 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85849988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-28DOI: 10.37895/2071-8004-2020-24-2-3-54-61
O. D. Lebedeva, I. A. Bokova, M. V. Rodkina, G. A. Lebedev
Purpose . To study the effectiveness of laser therapy in patients with cardiovascular diseases using a diagnostic hardware-software complex. Material and methods . 120 patients with cardiovascular diseases (arterial hypertension – AH and ischemic heart disease – IHD) were taken into the study. Patients were divided into three groups depending on the rehabilitation program and prescribed basic medicamentous therapy: Group I – reflexotherapy (RT); Group II – RT and laser therapy (RT + LT); Group III (controls) – basic medicamentous treatment. At reflexotherapy sessions, biologically active points were electrostimulated with pointed pulses from «Lasper» device (Japan) having amplitude 0.8–2.5 V, pulse duration 1.5 ms, frequency 10 Hz and exposure 20 minutes. Infrared «Mlada» apparatus, generating in continuous mode with wavelength 0.85 μm, output power of two emitters 30 mW was used for laser therapy sessions. The effectiveness of treatment was assessed with diagnostic hardware and software complex (APK) «Physiocontrol-R» (Russia) which includes: analyzer of heart rate variability by cardiointervalogram, hemodynamic analyzer, psychological testing program (Spielberger, Beck, SAN test). The renin-angiotensin-aldosterone system (RAAS) and sympatho-adrenal system (SAS) were assessed with biochemical analyzer «Chemetrics' (USA). The developed calculation algorithms were used for assessing organism's functional reserves and risks of developing common diseases. Discriminant equations derived by us were used to assess the effectiveness of rehabilitation measures and to define groups of patients with varying level of effectiveness. Results . General mechanisms of RT and LT therapeutic effects in patients with cardiovascular diseases have been found out: correction of SAS and RAAS activity, limitation of excessive sympathetic impacts at heart and restoration of autonomic regulation function of the cardiovascular system, as a whole, which has been confirmed by the dynamics of biochemical parameters and SAS and RAAC decrease. Application of RT + LT complex for the rehabilitation of patients with hypertension and ischemic heart disease is more effective compared to RT monotherapy, which is confirmed by the largest increase in loading threshold (by 49.4%) when analyzing findings of the exercise test. Conclusion . 1. RT + LT complex applied in patients with cardiovascular diseases for rehabilitation is more effective modality in comparison with RT monotherapy. 2. The hardware-software complex «Physiocontrol-R» has been found to be a possible tool for evaluating the effectiveness of non-medicamentous therapy. 3. The developed diagnostic modality: algorithms for calculating a quantitative assessment of body's functional reserves and defining risks of common diseases as well as the discriminant equations for determining the level of effectiveness in different groups of patients – can be widely used for assessing the effectiveness of treatment.
{"title":"Effectiveness of physical rehabilitation techniques with laser light in cardiovascular diseases under the control of a new diagnostic tools","authors":"O. D. Lebedeva, I. A. Bokova, M. V. Rodkina, G. A. Lebedev","doi":"10.37895/2071-8004-2020-24-2-3-54-61","DOIUrl":"https://doi.org/10.37895/2071-8004-2020-24-2-3-54-61","url":null,"abstract":"Purpose . To study the effectiveness of laser therapy in patients with cardiovascular diseases using a diagnostic hardware-software complex. Material and methods . 120 patients with cardiovascular diseases (arterial hypertension – AH and ischemic heart disease – IHD) were taken into the study. Patients were divided into three groups depending on the rehabilitation program and prescribed basic medicamentous therapy: Group I – reflexotherapy (RT); Group II – RT and laser therapy (RT + LT); Group III (controls) – basic medicamentous treatment. At reflexotherapy sessions, biologically active points were electrostimulated with pointed pulses from «Lasper» device (Japan) having amplitude 0.8–2.5 V, pulse duration 1.5 ms, frequency 10 Hz and exposure 20 minutes. Infrared «Mlada» apparatus, generating in continuous mode with wavelength 0.85 μm, output power of two emitters 30 mW was used for laser therapy sessions. The effectiveness of treatment was assessed with diagnostic hardware and software complex (APK) «Physiocontrol-R» (Russia) which includes: analyzer of heart rate variability by cardiointervalogram, hemodynamic analyzer, psychological testing program (Spielberger, Beck, SAN test). The renin-angiotensin-aldosterone system (RAAS) and sympatho-adrenal system (SAS) were assessed with biochemical analyzer «Chemetrics' (USA). The developed calculation algorithms were used for assessing organism's functional reserves and risks of developing common diseases. Discriminant equations derived by us were used to assess the effectiveness of rehabilitation measures and to define groups of patients with varying level of effectiveness. Results . General mechanisms of RT and LT therapeutic effects in patients with cardiovascular diseases have been found out: correction of SAS and RAAS activity, limitation of excessive sympathetic impacts at heart and restoration of autonomic regulation function of the cardiovascular system, as a whole, which has been confirmed by the dynamics of biochemical parameters and SAS and RAAC decrease. Application of RT + LT complex for the rehabilitation of patients with hypertension and ischemic heart disease is more effective compared to RT monotherapy, which is confirmed by the largest increase in loading threshold (by 49.4%) when analyzing findings of the exercise test. Conclusion . 1. RT + LT complex applied in patients with cardiovascular diseases for rehabilitation is more effective modality in comparison with RT monotherapy. 2. The hardware-software complex «Physiocontrol-R» has been found to be a possible tool for evaluating the effectiveness of non-medicamentous therapy. 3. The developed diagnostic modality: algorithms for calculating a quantitative assessment of body's functional reserves and defining risks of common diseases as well as the discriminant equations for determining the level of effectiveness in different groups of patients – can be widely used for assessing the effectiveness of treatment.","PeriodicalId":10008,"journal":{"name":"Chinese Journal of Laser Medicine & Surgery","volume":"38 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87854163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-28DOI: 10.37895/2071-8004-2020-24-2-3-90-94
R. V. Gallyamutdinov, L. Astakhova, E. S. Golovneva, O. U. Serysheva
Laser light can stimulate cell proliferation and restoration of various tissues after injury. The age aspect of laser irradiation effects at muscle regeneration, in particular the response of myosatellitocytes and myocytes, remains unexplored. The purpose of the research was to study the effect of laser light at the area of nuclei and the number of myosatellitocytes, as well as the area of myocyte nuclei in a model of posttraumatic regeneration of skeletal muscle in animals of different age groups. Material and methods . 32 laboratory mongrel rats, divided into 2 groups – young (3 months) and old (30 months) – were taken into the experiment. The formed surgical injuries at the caviar muscle on the left limb were experimental; they were irradiated with laser light (1060 nm, power 2.0 W, continuous mode, exposure 60 s, one time); injuries on the right limb were dynamic control. The animals were removed from the experiment on days 7 and 14. Tissue samples were fixed in formalin, histological sections were prepared, hematoxylin-eosin and Van Gizon picrofuxin were stained, and a digital image of objects was morphometrically analyzed. Results . It was found that after laser irradiation of the muscle, the area of nuclei and the number of myosatellites increase significantly compared to the control group, regardless of animal’s age. The response to myocyte nuclei to laser light was seen in both age groups, but it was significantly higher in younger animals. The obtained results demonstrate that laser radiation has the potential to activate muscle regeneration regardless of animal’s age.
激光能刺激细胞增殖和损伤后各种组织的修复。激光照射对肌肉再生的年龄方面的影响，特别是肌卫星细胞和肌细胞的反应，仍未被探索。本研究的目的是研究激光照射对不同年龄组动物创伤后骨骼肌再生模型的细胞核面积、肌卫星细胞数量以及肌细胞核面积的影响。材料和方法。实验选用32只实验用杂种大鼠，分为幼龄(3月龄)和老年(30月龄)2组。左肢鱼子酱肌形成的手术损伤是实验性的;用激光(1060 nm，功率2.0 W，连续模式，曝光60 s，一次)照射;右肢损伤为动态控制性。在第7天和第14天离开实验。组织标本用福尔马林固定，制作组织切片，苏木精-伊红和Van Gizon picrofuxin染色，并对物体的数字图像进行形态计量学分析。结果。结果发现，与对照组相比，激光照射肌肉后，无论动物年龄大小，细胞核面积和肌卫星数量均显著增加。肌细胞核对激光的反应在两个年龄组中都有，但在年轻的动物中明显更高。所得结果表明，激光辐射具有激活肌肉再生的潜力，而与动物的年龄无关。
{"title":"Effects of infrared laser radiation on qualitative and quantitative indices of a regenerating skeletal muscle in the age aspect","authors":"R. V. Gallyamutdinov, L. Astakhova, E. S. Golovneva, O. U. Serysheva","doi":"10.37895/2071-8004-2020-24-2-3-90-94","DOIUrl":"https://doi.org/10.37895/2071-8004-2020-24-2-3-90-94","url":null,"abstract":"Laser light can stimulate cell proliferation and restoration of various tissues after injury. The age aspect of laser irradiation effects at muscle regeneration, in particular the response of myosatellitocytes and myocytes, remains unexplored. The purpose of the research was to study the effect of laser light at the area of nuclei and the number of myosatellitocytes, as well as the area of myocyte nuclei in a model of posttraumatic regeneration of skeletal muscle in animals of different age groups. Material and methods . 32 laboratory mongrel rats, divided into 2 groups – young (3 months) and old (30 months) – were taken into the experiment. The formed surgical injuries at the caviar muscle on the left limb were experimental; they were irradiated with laser light (1060 nm, power 2.0 W, continuous mode, exposure 60 s, one time); injuries on the right limb were dynamic control. The animals were removed from the experiment on days 7 and 14. Tissue samples were fixed in formalin, histological sections were prepared, hematoxylin-eosin and Van Gizon picrofuxin were stained, and a digital image of objects was morphometrically analyzed. Results . It was found that after laser irradiation of the muscle, the area of nuclei and the number of myosatellites increase significantly compared to the control group, regardless of animal’s age. The response to myocyte nuclei to laser light was seen in both age groups, but it was significantly higher in younger animals. The obtained results demonstrate that laser radiation has the potential to activate muscle regeneration regardless of animal’s age.","PeriodicalId":10008,"journal":{"name":"Chinese Journal of Laser Medicine & Surgery","volume":"28 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91247105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-09-05DOI: 10.37895/2071-8004-2020-24-1-18-25
I. A. Mamedyarova
Purpose: To study a combined application of kinesis and laser therapy for correcting regional hemodynamic disorders in patients having dilatation cardiomyopathy (DCMP) with simultaneous supportive pharmaceutical therapy.Material and methods.100 patients with a verified diagnosis of DCMP were taken into the study. All patients had a differentiated supportive pharmtherapy. In three months after selection of the supportive differentiated drug therapy, patients were divided into two groups comparable by gender, age, disease course, severity of state and ways of medicine administration. Patients from Group 1, in addition to the supporting differentiated drug therapy, were given intravenous laser blood irradiation (ILBI) and unloading physical exercises. Patients from Group 2 continued their course of differentiated drug therapy.Research techniques included: clinical and functional observations; venous-occlusive plethysmography for assessing regional hemodynamics with a generally accepted method: measurement of blood flow (Qr) and regional vascular resistance (Rr) at rest; venous tone (Vt), reserve blood flow (QH) and regional vascular resistance (RH) under the functional loading test.Results.The data obtained during dynamic observations (in 1, 3, 6, 9 and 12 months) in Group 1 demonstrated a significant increase in volumetric blood flow velocity at rest (Qr) and reserve blood flow (QH); decrease of the regional vascular resistance at rest (Rr) and under functional loading (RH) as well as the decrease of venous tone (Vt), respectively. In Group 2 (controls), there were no significant positive dynamics; moreover, regional hemodynamics significantly worsened in 9 and 12 months.Conclusion. By the findings of venous-occlusive plethysmography, regional hemodynamics significantly improved in patients with DCMP under unloading therapeutic gymnastics in combination with ILBI and correctly selected differentiated drug therapy. The developed curative technique can be used in medical practice by GPs, therapists, cardiologists for optimizing treatment of patients with DCMP.
{"title":"Combined application of kinesis and laser therapy for the correction of disorders in regional hemodynamics at the dilatation cardiomyopathy","authors":"I. A. Mamedyarova","doi":"10.37895/2071-8004-2020-24-1-18-25","DOIUrl":"https://doi.org/10.37895/2071-8004-2020-24-1-18-25","url":null,"abstract":"Purpose: To study a combined application of kinesis and laser therapy for correcting regional hemodynamic disorders in patients having dilatation cardiomyopathy (DCMP) with simultaneous supportive pharmaceutical therapy.Material and methods.100 patients with a verified diagnosis of DCMP were taken into the study. All patients had a differentiated supportive pharmtherapy. In three months after selection of the supportive differentiated drug therapy, patients were divided into two groups comparable by gender, age, disease course, severity of state and ways of medicine administration. Patients from Group 1, in addition to the supporting differentiated drug therapy, were given intravenous laser blood irradiation (ILBI) and unloading physical exercises. Patients from Group 2 continued their course of differentiated drug therapy.Research techniques included: clinical and functional observations; venous-occlusive plethysmography for assessing regional hemodynamics with a generally accepted method: measurement of blood flow (Qr) and regional vascular resistance (Rr) at rest; venous tone (Vt), reserve blood flow (QH) and regional vascular resistance (RH) under the functional loading test.Results.The data obtained during dynamic observations (in 1, 3, 6, 9 and 12 months) in Group 1 demonstrated a significant increase in volumetric blood flow velocity at rest (Qr) and reserve blood flow (QH); decrease of the regional vascular resistance at rest (Rr) and under functional loading (RH) as well as the decrease of venous tone (Vt), respectively. In Group 2 (controls), there were no significant positive dynamics; moreover, regional hemodynamics significantly worsened in 9 and 12 months.Conclusion. By the findings of venous-occlusive plethysmography, regional hemodynamics significantly improved in patients with DCMP under unloading therapeutic gymnastics in combination with ILBI and correctly selected differentiated drug therapy. The developed curative technique can be used in medical practice by GPs, therapists, cardiologists for optimizing treatment of patients with DCMP.","PeriodicalId":10008,"journal":{"name":"Chinese Journal of Laser Medicine & Surgery","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83241758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-09-05DOI: 10.37895/2071-8004-2020-24-1-34-38
A. Lychagin, A. Garkavi, S. Ivannikov, O. Islaieh
Purpose. To improve the cure of knee osteoarthirtis using a combination of arthroscopic laser surgery and intraosseous infiltration of autologous platelet-rich plasma (PRP). Material and methods. 32 patients (18 men and 14 women) with osteoarthrosis (OA) of the knee joint, degree II–III (by Kellgren–Lawrence classification), participated in the study. By MRI findings, all patients had meniscus and cartilage damage. The arthroscopic intervention was done with laser device «LSP IRE Polyus», wavelength 0.97 mkm. In addition to the arthroscopy, patients were made extra-articular intraosseous infiltration of autologous platelet-rich plasma topically into the altered subchondral bone under the control of an electronic optical transducer (OEC Fluorostar 7900 COMPACT 2). The evaluation of treatment efficacy was made using WOMAC and KOOS knee function assessment scales, as well as VAS scale (pain intensity) before surgery and 1 and 3 months after the treatment beginning. Results. In 3 months, WOMAC scale showed the improved knee joint function by 25.38 points, KOOS scale – by 37.4 points and VAS scale – by 35 points (p < 0.01). Thus, the performed curative technique increased the support capacity, range of motion in the knee joint, less pain – all these contributed to the achievement of prolonged effect.
{"title":"Arthroscopic laser surgery combined with intraosseous infiltration of autologous platelet-rich plasma in the treatment of knee osteoarthritis","authors":"A. Lychagin, A. Garkavi, S. Ivannikov, O. Islaieh","doi":"10.37895/2071-8004-2020-24-1-34-38","DOIUrl":"https://doi.org/10.37895/2071-8004-2020-24-1-34-38","url":null,"abstract":"Purpose. To improve the cure of knee osteoarthirtis using a combination of arthroscopic laser surgery and intraosseous infiltration of autologous platelet-rich plasma (PRP). Material and methods. 32 patients (18 men and 14 women) with osteoarthrosis (OA) of the knee joint, degree II–III (by Kellgren–Lawrence classification), participated in the study. By MRI findings, all patients had meniscus and cartilage damage. The arthroscopic intervention was done with laser device «LSP IRE Polyus», wavelength 0.97 mkm. In addition to the arthroscopy, patients were made extra-articular intraosseous infiltration of autologous platelet-rich plasma topically into the altered subchondral bone under the control of an electronic optical transducer (OEC Fluorostar 7900 COMPACT 2). The evaluation of treatment efficacy was made using WOMAC and KOOS knee function assessment scales, as well as VAS scale (pain intensity) before surgery and 1 and 3 months after the treatment beginning. Results. In 3 months, WOMAC scale showed the improved knee joint function by 25.38 points, KOOS scale – by 37.4 points and VAS scale – by 35 points (p < 0.01). Thus, the performed curative technique increased the support capacity, range of motion in the knee joint, less pain – all these contributed to the achievement of prolonged effect.","PeriodicalId":10008,"journal":{"name":"Chinese Journal of Laser Medicine & Surgery","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75068528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-09-05DOI: 10.37895/2071-8004-2020-24-1-39-44
E. A. Leontiev, Yu. A. Igonin
Purpose: To study the effectiveness of tattoo pigment removal with laser light depending on the wavelength and depth of penetration into tissues in order to optimize a technique of laser selective photocavitation. Material and methods. 127 male white mongrel rats, aged 8 weeks, were intradermally injected with pigment particles into their backs looking like 2 rows of spots 0.5 cm in diameter. In 6 weeks, 367 skin samples with tattoo pigment were taken. Each sample was a patch of epidermis with pigment crystals surrounded by connective tissue capsules not less than 2.5 mm of thickness. Before the experiment, the epidermal stratum corneum – 10–15 mkm in depth and about 1 mm in diameter- was removed with spray-coagulation (apparatus EHVCH-50-MEDSI). The rest of skin flap surface remained intact. Thus, each skin sample had two areas on the surface – one with removed stratum corneum (experimental) and the other one intact (control). To register changes in the luminous flux, the authors placed an emitter (IPL xenon lamp 7.65.130), tissue sample and photomultiplier (PMT-62) on one and the same axis. To cut off light waves, the authors used a set of light filters – 315, 364, 400, 440, 490, 540, 590, 670, 750, 870, 980 nm. Results. Destruction of skin surface layers was not statistically significant under wavelengths up to 450 nm and after 1000 nm. The epidermal stratum corneum prevents laser light penetration with wavelengths 450–694 nm by 27%, in average, and with wavelengths 700–1000 nm by 33%, in average. Conclusion. Epidermal stratum corneum destruction statistically significantly increases light density in deep tissue layers and increases the depth of penetration of laser light into biological tissues.
{"title":"Optimization of laser technology for removing tatuage pigment","authors":"E. A. Leontiev, Yu. A. Igonin","doi":"10.37895/2071-8004-2020-24-1-39-44","DOIUrl":"https://doi.org/10.37895/2071-8004-2020-24-1-39-44","url":null,"abstract":"Purpose: To study the effectiveness of tattoo pigment removal with laser light depending on the wavelength and depth of penetration into tissues in order to optimize a technique of laser selective photocavitation. Material and methods. 127 male white mongrel rats, aged 8 weeks, were intradermally injected with pigment particles into their backs looking like 2 rows of spots 0.5 cm in diameter. In 6 weeks, 367 skin samples with tattoo pigment were taken. Each sample was a patch of epidermis with pigment crystals surrounded by connective tissue capsules not less than 2.5 mm of thickness. Before the experiment, the epidermal stratum corneum – 10–15 mkm in depth and about 1 mm in diameter- was removed with spray-coagulation (apparatus EHVCH-50-MEDSI). The rest of skin flap surface remained intact. Thus, each skin sample had two areas on the surface – one with removed stratum corneum (experimental) and the other one intact (control). To register changes in the luminous flux, the authors placed an emitter (IPL xenon lamp 7.65.130), tissue sample and photomultiplier (PMT-62) on one and the same axis. To cut off light waves, the authors used a set of light filters – 315, 364, 400, 440, 490, 540, 590, 670, 750, 870, 980 nm. Results. Destruction of skin surface layers was not statistically significant under wavelengths up to 450 nm and after 1000 nm. The epidermal stratum corneum prevents laser light penetration with wavelengths 450–694 nm by 27%, in average, and with wavelengths 700–1000 nm by 33%, in average. Conclusion. Epidermal stratum corneum destruction statistically significantly increases light density in deep tissue layers and increases the depth of penetration of laser light into biological tissues.","PeriodicalId":10008,"journal":{"name":"Chinese Journal of Laser Medicine & Surgery","volume":"246 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74018470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-09-05DOI: 10.37895/2071-8004-2020-24-1-9-17
V. Kozlov, V. Astashov
The authors present a review of publications in the scientific and medical literature on modern concepts of mechanisms of low-level laser biostimulating effects at the human body, at microvasculature chains and lymphoid organs. The main aspect of microcirculation photoactivation is the process of microcirculation reconstruction which leads to prolonged improvement in tissue trophism. There are three processes in the basement of biostimulating effect of low-level laser light in the red and near infrared spectral regions at microcirculation: hemomicrocirculation enhancement, activation of capillary neovascularization, lymphocorrection effects at the structure and functional activity of lymphoid organs.
{"title":"Photoactive effects of low-intensity laser irradiation at the microcirculation system and lymphoid organs","authors":"V. Kozlov, V. Astashov","doi":"10.37895/2071-8004-2020-24-1-9-17","DOIUrl":"https://doi.org/10.37895/2071-8004-2020-24-1-9-17","url":null,"abstract":"The authors present a review of publications in the scientific and medical literature on modern concepts of mechanisms of low-level laser biostimulating effects at the human body, at microvasculature chains and lymphoid organs. The main aspect of microcirculation photoactivation is the process of microcirculation reconstruction which leads to prolonged improvement in tissue trophism. There are three processes in the basement of biostimulating effect of low-level laser light in the red and near infrared spectral regions at microcirculation: hemomicrocirculation enhancement, activation of capillary neovascularization, lymphocorrection effects at the structure and functional activity of lymphoid organs.","PeriodicalId":10008,"journal":{"name":"Chinese Journal of Laser Medicine & Surgery","volume":"43 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73864389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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