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Screening of endophytic actinomycetes for their herbicidal activity 内生放线菌除草活性的筛选
Pub Date : 2018-06-01 DOI: 10.1016/j.aasci.2017.11.001
Helly Singh , Bindu Naik , Vijay Kumar , Gajraj Singh Bisht

Endophytic actinomycetes were isolated from 6 different plants collected from Uttarakhand, India. Actinomycetes isolates were screened for their herbicidal activity against Parthenium hysterophorus, Ageratum conyzoides and Bidens biternata. These isolates belong to the seven different genera. None of the isolate showed herbicidal activity on SCN agar plates. Therefore, direct fermentation method was used for the phytotoxin production in the submerged culture to study the herbicidal activity against different test weeds. The significant differences were observed in the production of phytoxin in SCN and GS medium. In the case of Ageratum conyzoides (billygoat weed), the culture filtrate of Nocardiodes sp. 1, Nocardiodes sp.2 and Actinomadura sp. showed 60% reduction in seed germination. However, culture filtrate of endophytic actinomycetes was ineffective on seed of Bidens biternata in the pre-emergence condition. In Parthenium hysterophorus, the highest seed germination reduction was observed with the culture filtrate (in SCN) of Nocardiodes sp.1 (80%). While in GS medium, maximum inhibition was observed in culture filtrate of Saccharopolyspora sp. (80%). The culture filtrates exhibited phytotoxic activity towards the germinated seedlings of Ageratum conyzoides, Bidens biternata, and Parthenium hysterophorus showing effects like leaf curling, wilting and burning. In post-emergence condition, reduction in shoot-root length was observed with the culture filtrate of Actinomadura sp.1, Nocardiodes sp.1, Streptomyces sp.1, Saccharopolyspora sp. and Microbispora sp. These findings suggest that endophytic actinomycetes are rich source of herbicidal metabolites. Further studies are required to isolate, purify and structure elucidation of the metabolites.

从印度北阿坎德邦6种不同植物中分离到内生放线菌。对分离的放线菌进行了除草活性的筛选,以测定其对子宫Parthenium hysterophorus、Ageratum conyzoides和Bidens biternata的除草活性。这些分离株属于7个不同的属。这些分离物在SCN琼脂板上均无除草活性。因此,采用直接发酵法在潜水培养中产生植物毒素,研究其对不同试验杂草的除草活性。在SCN和GS培养基中,植毒素的产生有显著差异。在灰山羊Ageratum conyzoides (billygoat weed)中,Nocardiodes sp. 1、Nocardiodes sp.2和Actinomadura sp.的培养滤液使种子萌发率降低60%。而内生放线菌培养滤液在出苗期前对双叶拜登种子无效。在子宫草中,Nocardiodes sp.1的培养滤液(SCN)降低种子萌发率最高(80%)。而在GS培养基中,Saccharopolyspora sp.培养滤液的抑制作用最大(80%)。培养滤液对鸡爪草(Ageratum conyzoides)、双叶蔷薇(Bidens biternata)和虎耳草(Parthenium hysterophorus)发芽苗表现出卷曲、萎蔫和灼烧的毒性作用。在出苗后,放线菌、Nocardiodes、Streptomyces、Saccharopolyspora sp、Microbispora sp的培养过滤液使芽根长度减少,说明内生放线菌是除草剂代解物的丰富来源。对其代谢产物的分离、纯化和结构分析有待进一步的研究。
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引用次数: 11
Peculiarities of the composition of acids in Sphagnum species of the percolation bog of the Kolkheti lowland 科尔赫提低地渗滤沼泽泥炭属植物中酸组成的特点
Pub Date : 2018-06-01 DOI: 10.1016/J.AASCI.2018.04.012
Natela Tetemadze, A. Bakuridze, M. Jokhadze, Izolda Machutadze
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引用次数: 5
Phenoloxidases of perennial plants: Hydroxylase activity, isolation and physiological role 多年生植物酚氧化酶:羟化酶活性、分离和生理作用
Pub Date : 2018-06-01 DOI: 10.1016/j.aasci.2018.03.009
N.T. Omiadze, N.I. Mchedlishvili, M.O. Abutidze

The difficulties of isolation of phenoloxidases with hydroxylase activity from different plants have been discussed. Phenoloxidase with high hydroxylase activity has been isolated from tea and kivi leaves, apple (Kekhura) fruits, walnut husks and vine stems. The tea leaf phenoloxidase has been shown to be able to catalyze the hydoxylation of the natural substrate p-coumaric acid. The step in the secondary metabolism in which phenoloxidase hydroxylase activity may be included in the plant lignification process has been shown. It has been supposed that masking of hydroxylase activity of phenoloxidase might be connected with the difficulties and peculiarities of the enzyme isolation and perhaps, hydroxylase activity of perennial plant phenoloxidase is included in the process of lignin formation.

讨论了从不同植物中分离具有羟化酶活性的酚氧化酶的难点。从茶叶和猕猴桃叶、苹果果实、核桃壳和藤茎中分离出具有高羟化酶活性的酚氧化酶。茶叶酚氧化酶已被证明能够催化天然底物对香豆酸的羟基化。在次生代谢的步骤中,酚氧化酶羟化酶活性可能包括在植物木质素化过程中。据推测,苯酚氧化酶的羟化酶活性被掩盖可能与酶分离的困难和特性有关,多年生植物酚氧化酶的羟化酶活性可能包含在木质素形成过程中。
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引用次数: 3
From the history of agrarian thought 从农业思想史来看
Pub Date : 2018-06-01 DOI: 10.1016/j.aasci.2018.04.010
Avtandil Silagadze

Agriculture has long been recognized as the main field of production in Egypt, Babylon, China, India … It is not accidental that we still have the most precious materials on the issues of management of this sphere, which have not lost their sensitivity to the present day. The study analyzes the individual aspects of the problem mainly on the example of ancient countries, particularly Rome. Based on the conclusion: For Platon the main thing is: The existence of natural farmland where everything is made according to demand; The land is the main sign - “mother”, which is the property of the “ideal state”. About the role of farming, Xenophon had a similar view, who first used the term “economics” and Aristotle, whose ownership is the term “economic science”; The issues of Latifundian agrarian farming research gained particular attention to Antiquity in Rome, where the specialists of Roman thinkers clearly outlined that the main field of agriculture, and the income derived from it - is the most sacred; The most effective product is near the selling markets; The importance of livestock, viticulture and gardening took place in the foreground; The objective of the science of farming is to study soil composition, selection of favorable time for land-processing tools and land cultivation; Additional work used in land cultivation ensures receiving of surplus product … Despite the experience of history, unfortunately, in post-Soviet Georgia does not pay much attention to the development of real economy, including agriculture sector.

农业长期以来一直被认为是埃及、巴比伦、中国、印度的主要生产领域……我们仍然拥有关于这一领域管理问题的最宝贵的资料,这并非偶然,这些资料至今仍未失去其敏感性。该研究主要以古代国家,特别是罗马为例,分析了问题的各个方面。对柏拉图来说,主要的是:自然农田的存在,一切都是按需生产的;土地是主要的标志——“母亲”,是“理想状态”的财产。关于农业的作用,色诺芬有类似的观点,他首先使用了“经济学”一词,亚里士多德的所有权是“经济科学”一词;大庄园式农业研究的问题在古罗马得到了特别的关注,罗马思想家的专家们清楚地概述了农业的主要领域,以及从中获得的收入——是最神圣的;最有效的产品在销售市场附近;畜牧业、葡萄栽培和园艺的重要性占据了前景;农学的目的是研究土壤成分,选择土地加工工具和土地耕作的有利时机;在土地耕作中使用额外的工作确保获得剩余产品……尽管有历史经验,不幸的是,后苏联格鲁吉亚不太关注实体经济的发展,包括农业部门。
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引用次数: 1
Simulative modeling of the soil erosion processes 土壤侵蚀过程的模拟模拟
Pub Date : 2018-06-01 DOI: 10.1016/j.aasci.2018.03.007
Irina Khubulava, Goga Chakhaia

In order to visualize the negative consequences of soil erosion processes running in Georgia, namely in the Gldaniskhevi ravine within the territory of Tbilisi, research field integrated polygon has been arranged on the eroded slope chosen beforehand.

At the control area of polygon 24 field experiments have been carried out to study the dynamics of the formed erosion processes under the different intensity precipitation conditions, whereby scour parameters were being measured (average depth – h, average width – b, B).

For estimating the exactness of calculations done on the results of measurings accomplished with the purpose to definite the intensity of soil erosion processes, the method of least squares have been used, on the base of which contour dependence between investigated parameters and therefore validity of the accomplished researches have been ascertained.

Simulative modeling of the soil erosion processes has been accomplished by means of special computer programs, which has shown, that during 167 days at the control area of the field polygon the eroded slope gulleying processes have been significantly strengthened, that nowadays can be also noticed on the slopes outside the polygon, on account of which can be concluded, that in order, to prevent expected landslide and debris flow phenomena in the river Gldaniskhevi ravine, implementation of urgent effective anti-erosive measures is needed.

为了可视化格鲁吉亚境内土壤侵蚀过程的负面影响,即第比利斯境内的Gldaniskhevi峡谷,在事先选择的侵蚀斜坡上布置了研究场地综合多边形。在24多边形控制区进行了不同强度降水条件下形成的侵蚀过程的动态试验,测量了冲刷参数(平均深度- h,平均宽度- b, b)。为了估计测量结果计算的准确性,确定了土壤侵蚀过程的强度,采用了最小二乘法。在此基础上,确定了所研究参数之间的轮廓相关性,从而确定了所完成研究的有效性。利用专门的计算机程序对土壤侵蚀过程进行了模拟模拟,结果表明,在167天的时间里,在田间多角形控制区,侵蚀坡面冲沟过程明显加强,目前在多角形外的斜坡上也可以看到侵蚀坡面冲沟过程,因此可以得出结论,为了防止格达尼斯赫维河沟壑区出现预期的滑坡和泥石流现象。急需采取有效的防腐蚀措施。
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引用次数: 1
The investigation of phenolic compounds and anthocyanins of wines made of the grape variety karmrahyut 卡姆拉厄特葡萄酿造的葡萄酒中酚类化合物和花青素的研究
Pub Date : 2018-06-01 DOI: 10.1016/j.aasci.2018.03.005
Artak Gabrielyan , Karen Kazumyan

Wine phenolics are important quality components that contribute to the color, taste and feel of wines. The majority of the phenolic constituents found in wine are grape-derived. In red wine, tannins and anthocyanins are the most important phenolic classes. Tannins contribute to the mouth feel of wines but they also form pigmented polymers in association with the anthocyanins to provide the stable pigments required to give red wine its long-term color stability [1,2]. Moreover, polyphenols also exert many favorable effects on human health, such as the inhibition of atherosclerosis, coronary heart disease and various cancer types [3]. By focusing on the above, our goal was to produce rose and red dry wines from Karmrahyut grape variety, which is a hybrid variety and is very little studied, and to investigate the content of anthocyanins, total phenols and monomer index in them.

葡萄酒酚类物质是重要的品质成分,有助于葡萄酒的颜色,味道和感觉。葡萄酒中发现的大部分酚类成分都是从葡萄中提取的。在红酒中,单宁和花青素是最重要的酚类物质。单宁有助于葡萄酒的口感,但它们也与花青素形成色素聚合物,为红葡萄酒提供长期颜色稳定性所需的稳定色素[1,2]。此外,多酚对人体健康也有许多有益的作用,如抑制动脉粥样硬化、冠心病和各种癌症。在此基础上,我们的目标是利用研究较少的杂交品种karrahyut葡萄品种生产桃红和干红葡萄酒,并研究其花青素、总酚和单体指数的含量。
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引用次数: 7
From the history of agrarian thought 从农业思想史来看
Pub Date : 2018-06-01 DOI: 10.1016/J.AASCI.2018.04.010
Avtandil Silagadze
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引用次数: 1
Sequential extraction of bioactive compounds from tangerine (Citrus Unshiu) peel 柑桔果皮生物活性物质的序贯提取
Pub Date : 2018-06-01 DOI: 10.1016/j.aasci.2018.02.007
Mzia Tsitsagi, Ketevan Ebralidze, Mariam Chkhaidze, Imeda Rubashvili, Vladimer Tsitsishvili

Tangerine (Citrus Unshiu) is one of important agricultural crops in Georgia. As the statistical data for year 2016 manifest, the total harvest exceeds 100 thousand tons. Tangerine peel, agro-industrial waste of production of juice concentrates and jams present rich sources of valuable bioactive compounds. Due to their high bioactive compound content, tangerine peels could be applied by both pharmaceutical and food industries. In spite of this, citrus peel is usually treated as by-products or waste, resulting in environmental pollution. Citrus peels are promising source of essential oil, carotenes, natural flavanones such as hesperidin and pectin. Stepwise, sequential utilization of tangerine peel, which provides an opportunity for selective extraction of the aforementioned bioactive compounds and allows one to manage nonstandard tangerine (amounting to a third of the harvest) in a rational manner. Hence, the present study is undertaken to elaborate a feasible and effective method of sequential extraction of above mentioned bioactive compounds from tangerine peel. The first step is supercritical CO2 extraction of essential oil. Optimal conditions for β-carotene free tangerine oil are 100 atm pressure, 35C0 temperature, and 15 min equilibrium time. The principal compound in Citrus Unshiu peel essential oil is d-limonene. Stepwise extraction requares correct extraction sequence of target products. Acetone (7%) was used as co-solvent in the second step of extraction. The β-carotene is soluble in acetone, whereas hesperidin is not. Optimal parameters are 150 atm, 40 °C and 1 h equilibrium, and 1 h extraction time in dynamic conditions. Methanol (7%) was used in the third step of extraction. Optimal parameters were: 250 atm pressure, 60 °C temperature, 1 h equilibrium and 30 min dynamic extraction time. At least pectin was extracted from residue of tangerin. Stepwise supercritical fluid extraction of bioactive compounds from agro-industrial waste materials is simple, effective, eco friendly separation method, which provides high quality of target products and needs one standard technological equipment.

橘子(Citrus Unshiu)是格鲁吉亚重要的农作物之一。2016年的统计数据显示,总收获量超过10万吨。柑桔皮、浓缩果汁和果酱生产过程中产生的农业工业废料是有价值的生物活性化合物的丰富来源。陈皮具有较高的生物活性成分,可用于制药和食品工业。尽管如此,柑橘皮通常被当作副产品或废物处理,造成环境污染。柑橘果皮是精油、胡萝卜素、橙皮苷和果胶等天然黄酮的重要来源。逐步地、顺序地利用陈皮,这为选择性地提取上述生物活性化合物提供了机会,并允许人们以合理的方式管理非标准橘子(约占收成的三分之一)。因此,本研究旨在建立一种可行而有效的从陈皮中依次提取上述生物活性物质的方法。第一步是超临界CO2萃取精油。无β-胡萝卜素柑桔油的最佳提取条件为压力100 atm,温度35℃,平衡时间15 min。柠檬皮精油的主要成分是d-柠檬烯。逐步提取要求正确的目标产物提取顺序。以丙酮(7%)为辅助溶剂进行第二步萃取。β-胡萝卜素可溶于丙酮,而橙皮苷则不能。最佳提取参数为150 atm, 40℃,平衡时间1 h,动态提取时间1 h。第三步提取采用甲醇(7%)。最佳提取参数为:压力250 atm,温度60℃,平衡时间1 h,动态提取时间30 min。从橘皮渣中至少提取出果胶。超临界分步萃取农工废弃物中生物活性物质是一种简单、有效、环保的分离方法,其目标产物质量高,且只需要一个标准的工艺设备。
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引用次数: 23
The investigation of phenolic compounds and anthocyanins of wines made of the grape variety karmrahyut 卡姆拉厄特葡萄酿造的葡萄酒中酚类化合物和花青素的研究
Pub Date : 2018-06-01 DOI: 10.1016/J.AASCI.2018.03.005
A. Gabrielyan, K. Kazumyan
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引用次数: 7
Patterns of distribution and survival of European yew ( Taxus baccata L.) in an alpine tree line ecotone in the Greater Caucasus (Georgia) 欧洲红豆杉(Taxus baccata L.)在大高加索(格鲁吉亚)高山林木线过渡带的分布和生存模式
Pub Date : 2018-06-01 DOI: 10.1016/J.AASCI.2018.02.006
A. Gegechkori
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引用次数: 6
期刊
Annals of Agrarian Science
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