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Evaluating the effect of soil grading on the UCS of MICP-treated sandy soils 评价土壤分级对micp处理砂质土壤UCS的影响
Pub Date : 2024-10-10 DOI: 10.1016/j.bgtech.2024.100144
Reena N. Hora , Md Mizanur Rahman , Md Rajibul Karim , Simon Beecham
Many past studies have investigated the Unconfined Compressive Strength (UCS) behaviour of MICP-treated sandy soils and developed empirical relationships to predict strength improvement. While the UCS of MICP-treated soils can be affected by many factors such as chemical concentration, temperature, and biochemistry, it has been found that particle size (d10) is one of the important contributing factors, but most of the previously published studies have not considered it. This study applied MICP on three different variants of Adelaide Industrial (AI) clean sands with different grain size distributions to evaluate the effect on UCS and Calcium Carbonate (CaCO3) precipitation. To better understand the influence of particle size, this study also collected literature data on UCS, CaCO3 content and soil grading properties. A numerical method was used to interpolate the distribution of the combined data (literature and experimental) in 3D space to establish a clear correlation between UCS, CaCO3 content (CC) and the soil grading properties. So, contour plots were generated between UCS, CC, and d10 and other soil grading properties. Where the 2D and 3D plots could not clearly present the influence of d10 on the variation of UCS and CC, contour plots presented the distribution rather clearly. The contour plots showed a visible trend in the variation of UCS and CC for d10 and Coefficient of Curvature (Cc), but not for Coefficient of Uniformity (Cu).
许多过去的研究调查了无侧限抗压强度(UCS)行为的micp处理砂土,并建立经验关系,以预测强度的提高。虽然micp处理土壤的UCS受化学浓度、温度和生物化学等多种因素的影响,但已经发现粒径(d10)是重要的影响因素之一,但大多数先前发表的研究都没有考虑到这一点。本研究将MICP应用于三种不同粒径分布的阿德莱德工业(AI)洁净砂,以评估其对UCS和碳酸钙(CaCO3)降水的影响。为了更好地了解粒径的影响,本研究还收集了关于UCS、CaCO3含量和土壤分级特性的文献数据。采用数值方法对文献和实验数据组合在三维空间中的分布进行插值,建立UCS、CaCO3含量(CC)与土壤级配特性之间的清晰相关性。因此,在UCS、CC和d10等土壤分级属性之间生成等高线图。2D和3D图不能清楚地反映d10对UCS和CC变化的影响,等高线图能较清楚地反映其分布。等高线图显示,d10和曲率系数(CC)的UCS和CC的变化趋势明显,而均匀度系数(Cu)的变化趋势不明显。
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引用次数: 0
Review of bio-enzyme for soil improvement 生物酶在土壤改良中的研究进展
Pub Date : 2024-10-09 DOI: 10.1016/j.bgtech.2024.100143
Yuhao Li , Xiangwei Fang , Chunni Shen , Wenchen Jiang , Sheng Huang , Guoliang Ma
Traditional soil stabilizers, such as cement and lime, typically entail substantial energy consumption and environmental pollution. In contrast, bio-enzyme has emerged as a promising alternative, aligning with the imperatives of sustainable development, cost-effectiveness, and environmental friendliness. Bio-enzymes are primarily one or more protein molecules that catalyzes chemical reactions in the soil to form a cementing bond that stabilizes the soil structure and reduces the soil’s affinity for water. Currently, a plethora of studies on bio-enzyme have been conducted by scholars worldwide, yet there remains a notable absence of the systematic organization and comprehensive review of these findings. This study offers a thorough examination of bio-enzyme technology, encompassing its biochemical properties, mechanisms, the engineering properties of stabilized soil, bio-enzymatic composites, and its engineering applications. And current trends and future prospects of bio-enzyme are also scrutinized. This forward-looking study indicates that bio-enzyme functions through mechanisms such as cation exchange, specific binding, and surfactants, among others to diminish the electric double layer thickness and hydrophilicity of soil, consequently enhancing engineering properties of soil. And the improvement performance can be influenced by various factors, including soil properties, enzyme dosage, specificity, and sample preparation, etc. It is also noted that the composites of bio-enzyme with conventional stabilizers tend to enhance improvement performance more efficiently. The engineering applications of bio-enzyme have demonstrated its superiority over traditional stabilizers in soil improvement. However, the performance of treated soils with available bio-enzyme remains limited, highlighting the necessity for extracting novel bio-enzyme form plants/animals and determining its mechanisms and engineering mechanical properties. It is also essential to develop more bio-enzymatic composites and conduct application in-situ to develop relevant standards and application guidelines.
传统的土壤稳定剂,如水泥和石灰,通常需要大量的能源消耗和环境污染。相比之下,生物酶已成为一种有前途的替代方案,符合可持续发展、成本效益和环境友好的要求。生物酶主要是一种或多种蛋白质分子,它们催化土壤中的化学反应,形成一种胶结键,稳定土壤结构,降低土壤对水的亲和力。目前,国内外学者对生物酶进行了大量的研究,但缺乏对这些研究结果进行系统的组织和全面的综述。本研究对生物酶技术进行了全面的研究,包括其生化特性、机理、稳定土壤的工程特性、生物酶复合材料及其工程应用。并对生物酶的发展趋势和前景进行了展望。这一前瞻性的研究表明,生物酶通过阳离子交换、特异性结合、表面活性剂等机制发挥作用,减少土壤的电双层厚度和亲水性,从而提高土壤的工程性能。改良效果受多种因素的影响,包括土壤性质、酶用量、特异性、样品制备等。同时指出,生物酶与常规稳定剂的复合可以更有效地提高改良性能。生物酶的工程应用证明了其在土壤改良方面的优越性。然而,可用的生物酶处理土壤的性能仍然有限,突出了从植物/动物中提取新型生物酶并确定其机制和工程力学特性的必要性。开发更多的生物酶复合材料并进行原位应用,制定相关标准和应用指南也是必不可少的。
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引用次数: 0
Effect of sticky rice on the strength and permeability of bio-cemented sand 糯米对生物胶结砂强度和渗透性的影响
Pub Date : 2024-09-30 DOI: 10.1016/j.bgtech.2024.100141
Qiyong Zhang , Jian Chu , Zheng Zhang , Wenhao Wang , Shifan Wu
Microbially induced carbonate precipitation (MICP) is an eco-friendly soil improvement technique. However, this method still has some drawbacks, such as low conversion efficiency of CaCO3 crystallization, insufficient strength for certain applications, and requiring multiple treatments. Previous studies have reported that sticky rice can regulate CaCO3 crystals (i.e., chemical CaCO3) in the sticky rice-lime mortar, showing potential for improving the bio-cementation. Therefore, this study explored the possibility of using sticky rice to enhance the biocementation effect. Tests were carried out to assess the strength and permeability of bio-cemented sand with the inclusion of sticky rice. The results indicated that sticky rice may regulate the type and size of bio-CaCO3 crystals, and the use of an appropriate amount of sticky rice as additive could increase the strength of sand columns by regulating CaCO3 crystallization. Polyhedral calcites may be more favourable for the increasing strength than some vaterites with a hollow spherical structure. The combination of MICP and sticky rice can significantly decrease the coefficient of permeability to a value that was much lower than that by using sticky rice and MICP alone. Bio-CaCO3 immobilized the sticky rice on one end on sand particles, and the reticulated structure of sticky rice divided large pores into small pores, which may be the important cause of the decrease in permeability coefficient. Finally, this study proposed that the MICP with the sticky rice as an additive may enhance the MICP effect and prevent the surface erosion of coarse-grained sand slopes.
微生物诱导碳酸盐降水(MICP)是一种生态友好型土壤改良技术。但该方法仍存在CaCO3结晶转化效率低、某些应用强度不足、需要多次处理等缺点。已有研究报道,糯米可以调节糯米-石灰砂浆中的CaCO3晶体(即化学CaCO3),显示出改善生物胶结的潜力。因此,本研究探讨了利用糯米增强生物胶结效果的可能性。研究了含糯米生物胶结砂的强度和渗透性。结果表明,糯米可以调节生物CaCO3结晶的类型和大小,添加适量的糯米可以通过调节CaCO3结晶来提高砂柱的强度。多面体方解石可能比某些具有空心球形结构的方解石更有利于提高强度。MICP与糯米复合可显著降低渗透系数,其渗透系数远低于单独使用糯米和MICP的渗透系数。Bio-CaCO3将糯米一端固定在沙粒上,糯米的网状结构将大孔分成小孔,这可能是导致渗透系数降低的重要原因。最后,本研究提出以糯米为添加剂的MICP可以增强MICP效应,防止粗粒砂坡面侵蚀。
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引用次数: 0
Miniaturized device to measure urease activity in the soil interstitial fluid using wenner method 利用温纳法测量土壤间隙液中尿素酶活性的微型装置
Pub Date : 2024-09-30 DOI: 10.1016/j.bgtech.2024.100120
Rafaela Cardoso , Thomas Drouinot , Susana Cardoso de Freitas
This paper presents a microdevice developed to measure the electrical conductivity of a liquid or a saturated porous medium using Wenner method. It is developed in the context of biocementation as soil improvement technique, which is used in Civil Engineering applications to produce calcium carbonate through bacterial or enzymatic activity, replacing the use of other binder materials such as cement or resins, and therefore reducing carbon footprint. The microdevice was used to measure urease activity in the soil interstitial fluid, to investigate if bacterial activity could be affected by the presence of the particles and tortuosity from pore geometry. Such analysis is important to understand biocementation mechanism inside the soil and helps to improve the design of such treatment solutions. The device is basically a squared reservoir printed in polypropylene using a 3D printing machine, incorporating stainless steel electrodes in its base. The electrical resistivity was computed adopting Wenner method, by connecting 4 PCB electrodes to a signal generator and an oscilloscope for measuring the voltage when a AC current of 1 mA was applied. Both square and sinusoidal waves with 5 kHz frequency were selected among other frequencies. The measurements were adjusted during the calibration of the microdevice, done using standard salt solutions with known electrical conductivity measured using an electrical conductivity probe. For the bacterial activity measurements, the bacterial and urea solutions were added to a uniform-graded size quarzitic sand (average diameter 0.3 mm) placed inside the microdevice and covering completely the electrodes. Bacterial activity was not affected by the presence of the sand, which confirms that this treatment is effective for this type of soils.
本文介绍了一种利用温纳法测量液体或饱和多孔介质电导率的微型装置。生物水泥化是一种土壤改良技术,应用于土木工程领域,通过细菌或酶的活性产生碳酸钙,取代水泥或树脂等其他粘结材料的使用,从而减少碳足迹。该微型装置用于测量土壤间隙流体中的脲酶活性,以研究细菌活性是否会受到颗粒存在和孔隙几何形状迂回的影响。此类分析对于了解土壤内部的生物降解机制非常重要,有助于改进此类处理方案的设计。该装置基本上是一个使用 3D 打印机用聚丙烯打印的方形蓄水池,其底部装有不锈钢电极。电阻率的计算采用温纳法,将 4 个 PCB 电极连接到信号发生器和示波器上,以测量施加 1 mA 交流电时的电压。在其他频率中,选择了频率为 5 kHz 的方波和正弦波。在校准微型设备时,使用标准盐溶液对测量结果进行调整,标准盐溶液的电导率是通过电导率探针测量得出的。在测量细菌活性时,将细菌溶液和尿素溶液加入放置在微装置内并完全覆盖电极的大小均匀的石英砂(平均直径 0.3 毫米)中。细菌活性并没有因为沙子的存在而受到影响,这证明这种处理方法对这类土壤是有效的。
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引用次数: 0
Thermal desorption remediation effects on soil biogeochemical properties and plant performance: Global meta-analysis 热解吸修复对土壤生物地球化学性质和植物性能的影响:全球荟萃分析
Pub Date : 2024-09-26 DOI: 10.1016/j.bgtech.2024.100140
Jiaxin Liao , Haowen Guo , Sanandam Bordoloi , Denian Li , Yuanxiang Zhang , Junjun Ni , Haoran Yuan , Xudong Zhao
Soil contamination remains a global problem, and numerous studies have been published for investigating soil remediation. Thermal desorption remediation (TDR) can significantly reduce the contaminants in the soil within a short time and consequently has been used worldwide. However, the soil properties respond to TDR differently and are dependent on the experimental set-up. The causative mechanisms of these differences are yet to be fully elucidated. A statistical meta-analysis was thus undertaken to evaluate the TDR treatment effects on soil properties and plant performance. This review pointed out that soil clay was reduced by 54.2%, while soil sand content was enhanced by 15.2% after TDR. This might be due to the release of cementing agents from clay minerals that resulted in the formation of soil aggregates. Soil electrical conductivity enhanced by 69.5% after TDR, which might be due to the heating-induced loss of structural hydroxyl groups and the consequent liberation of ions. The treatment of TDR leads to the reduction of plant germination rate, length, and biomass by 19.4%, 44.8%, and 20.2%, respectively, compared to that of control soil. This might be due to the residue of contaminants and the loss of soil fertility during the thermal process that inhibited plant germination and growth. Soil pH and sulfate content increased with heating temperature increased, while soil enzyme activities decreased with thermal temperature increased. Overall, the results suggested that TDR treatment has inhibited plant growth as well as ecological restoration.
土壤污染仍然是一个全球性问题,已经发表了许多关于土壤修复的研究。热解吸修复技术(TDR)能在短时间内显著降低土壤中的污染物含量,已在世界范围内得到广泛应用。然而,土壤性质对TDR的响应不同,并且取决于实验设置。这些差异的成因机制尚未完全阐明。因此,进行了统计荟萃分析,以评估TDR处理对土壤性质和植物性能的影响。结果表明,TDR后土壤粘粒含量减少54.2%,砂粒含量增加15.2%。这可能是由于粘土矿物释放胶结剂导致土壤团聚体的形成。TDR后土壤电导率提高了69.5%,这可能是由于加热导致结构羟基的损失和离子的释放。与对照土壤相比,TDR处理导致植物发芽率、长度和生物量分别下降19.4%、44.8%和20.2%。这可能是由于污染物的残留和热过程中土壤肥力的丧失,抑制了植物的发芽和生长。土壤pH和硫酸盐含量随加热温度升高而升高,土壤酶活性随加热温度升高而降低。综上所述,TDR处理抑制了植物生长和生态恢复。
{"title":"Thermal desorption remediation effects on soil biogeochemical properties and plant performance: Global meta-analysis","authors":"Jiaxin Liao ,&nbsp;Haowen Guo ,&nbsp;Sanandam Bordoloi ,&nbsp;Denian Li ,&nbsp;Yuanxiang Zhang ,&nbsp;Junjun Ni ,&nbsp;Haoran Yuan ,&nbsp;Xudong Zhao","doi":"10.1016/j.bgtech.2024.100140","DOIUrl":"10.1016/j.bgtech.2024.100140","url":null,"abstract":"<div><div>Soil contamination remains a global problem, and numerous studies have been published for investigating soil remediation. Thermal desorption remediation (TDR) can significantly reduce the contaminants in the soil within a short time and consequently has been used worldwide. However, the soil properties respond to TDR differently and are dependent on the experimental set-up. The causative mechanisms of these differences are yet to be fully elucidated. A statistical meta-analysis was thus undertaken to evaluate the TDR treatment effects on soil properties and plant performance. This review pointed out that soil clay was reduced by 54.2%, while soil sand content was enhanced by 15.2% after TDR. This might be due to the release of cementing agents from clay minerals that resulted in the formation of soil aggregates. Soil electrical conductivity enhanced by 69.5% after TDR, which might be due to the heating-induced loss of structural hydroxyl groups and the consequent liberation of ions. The treatment of TDR leads to the reduction of plant germination rate, length, and biomass by 19.4%, 44.8%, and 20.2%, respectively, compared to that of control soil. This might be due to the residue of contaminants and the loss of soil fertility during the thermal process that inhibited plant germination and growth. Soil pH and sulfate content increased with heating temperature increased, while soil enzyme activities decreased with thermal temperature increased. Overall, the results suggested that TDR treatment has inhibited plant growth as well as ecological restoration.</div></div>","PeriodicalId":100175,"journal":{"name":"Biogeotechnics","volume":"3 3","pages":"Article 100140"},"PeriodicalIF":0.0,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144557605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Climate impacts on deformation and instability of vegetated slopes 气候对植被边坡变形和失稳的影响
Pub Date : 2024-09-16 DOI: 10.1016/j.bgtech.2024.100139
Qi Zhang , Haiyi Zhong , Haowen Guo , Junjun Ni
Eco-geotechnical engineering plays a pivotal role in enhancing global sustainability and upholding the performance of earthen structures. The utilization of vegetation to stabilise geotechnical infrastructures is widely recognized and embraced for its environmentally friendly attributes. The spectre of climate change further intensifies the focus on the effects of temperature and humidity on vegetated soil. Consequently, there is a pressing need for research exploring the influence of changing climates on vegetated infrastructures. Such research demands a holistic and interdisciplinary approach, bridging fields such as soil mechanics, botany, and atmospheric science. This review underscores key facets crucial to vegetated geotechnical infrastructures, encompassing climate projections, centrifuge modelling, field monitoring, and numerical methodologies.
生态岩土工程在提高全球可持续性和维护土质结构的性能方面发挥着关键作用。利用植被稳定岩土基础设施因其环保特性而得到广泛认可和接受。气候变化的幽灵进一步加强了对温度和湿度对植被土壤影响的关注。因此,迫切需要研究气候变化对植被基础设施的影响。这样的研究需要一个整体和跨学科的方法,桥梁领域,如土壤力学,植物学和大气科学。这篇综述强调了植被岩土基础设施的关键方面,包括气候预测、离心机建模、现场监测和数值方法。
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引用次数: 0
Experimental investigation on response of biocemented coral sand pile composite foundation under seismic waves 地震波作用下生物胶凝珊瑚砂桩复合地基响应试验研究
Pub Date : 2024-09-07 DOI: 10.1016/j.bgtech.2024.100136
Xiangwei Fang , Chao Chen , Ganggang Zhou , Zhixiong Chen , Chunyan Wang , Luqi Wang
The biocemented coral sand pile composite foundation represents an innovative foundation improvement technology, utilizing Microbially Induced Carbonate Precipitation (MICP) to consolidate a specific volume of coral sand within the foundation into piles with defined strength, thereby enabling them to collaboratively bear external loads with the surrounding unconsolidated coral sand. In this study, a series of shaking table model tests were conducted to explore the dynamic response of the biocemented coral sand pile composite foundation under varying seismic wave types and peak accelerations. The surface macroscopic phenomena, excess pore water pressure ratio, acceleration response, and vertical settlement were measured and analysed in detail. Test results show that seismic wave types play a decisive role in the macroscopic surface phenomena and the response of the excess pore water pressure ratio. The cumulative settlement of the upper structure under the action of Taft waves was about 1.5 times that of El Centro waves and Kobe waves. The most pronounced liquefaction phenomena were recorded under the Taft wave, followed by the El Centro wave, and subsequently the Kobe wave. An observed positive correlation was established between the liquefaction phenomenon and the Aristotelian intensity of the seismic waves. However, variations in seismic wave types exerted minimal influence on the acceleration amplification factor of the coral sand foundation. Analysis of the acceleration amplification factor revealed a triphasic pattern—initially increasing, subsequently decreasing, and finally increasing again—as burial depth increased, in relation to the peak value of the input acceleration. This study confirms that the biocemented coral sand pile composite foundation can effectively enhance the liquefaction resistance of coral sand foundations.
生物胶结珊瑚砂桩复合地基是一种创新的地基改善技术,利用微生物诱导碳酸盐沉淀(MICP)将基础内一定体积的珊瑚砂固结成具有一定强度的桩,从而使其能够与周围未固结的珊瑚砂协同承受外部荷载。本研究通过一系列振动台模型试验,探讨了不同地震波类型和峰值加速度下生物水泥珊瑚砂桩复合地基的动力响应。对地表宏观现象、超孔隙水压力比、加速度响应和竖向沉降进行了详细的测量和分析。试验结果表明,地震波类型对宏观表面现象和超孔隙水压力比的响应起决定性作用。塔夫脱波作用下上部结构的累积沉降量约为El Centro波和Kobe波的1.5倍。最明显的液化现象是在塔夫脱波下记录的,其次是埃尔森特罗波,然后是神户波。观测到的液化现象与亚里斯多德地震波强度之间存在正相关关系。而地震波类型的变化对珊瑚砂地基加速度放大系数的影响较小。与输入加速度峰值相关的加速度放大因子分析显示,随着埋深的增加,加速度放大因子呈先增大后减小,最后再增大的三相模式。本研究证实了生物胶凝珊瑚砂桩复合地基可以有效增强珊瑚砂地基的抗液化能力。
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引用次数: 0
Architecture characterization of orchard trees for mechanical behavior investigations 果树机械行为研究的结构表征
Pub Date : 2024-08-31 DOI: 10.1016/j.bgtech.2024.100138
Min Kyung Jeon , Matthew Burrall , Tae Hyuk Kwon , Jason T. DeJong , Alejandro Martinez
Characterizing the architecture of tree root systems is essential to advance the development of root-inspired anchorage in engineered systems. This study explores the structural root architectures of orchard trees to understand the interplays between the mechanical behavior of roots and the root architecture. Full three-dimensional (3D) models of natural tree root systems, Lovell, Marianna, and Myrobalan, that were extracted from the ground by vertical pullout are reconstructed through photogrammetry and later skeletonized as nodes and root branch segments. Combined analyses of the full 3D models and skeletonized models enable a detailed examination of basic bulk properties and quantification of architectural parameters. While the root segments are divided into three categories, trunk root, main lateral root, and remaining roots, the patterns in branching and diameter distributions show significant differences between the trunk and main laterals versus the remaining lateral roots. In general, the branching angle decreases over the sequence of bifurcations. The main lateral roots near the trunk show significant spreading while the lateral roots near the ends grow roughly parallel to the parent root. For branch length, the roots bifurcate more frequently near the trunk and later they grow longer. Local thickness analysis confirms that the root diameter decays at a higher rate near the trunk than in the remaining lateral roots, while the total cross-sectional area across a bifurcation node remains mostly conserved. The histograms of branching angle, and branch length and thickness gradient can be described using lognormal and exponential distributions, respectively. This unique study presents data to characterize mechanically important structural roots, which may help link root architecture to the mechanical behaviors of root structures.
表征树根系统的结构对于推进工程系统中受根启发的锚固的发展至关重要。本研究通过对果树根系结构的研究,了解根系力学行为与根系结构之间的相互作用。Lovell、Marianna和Myrobalan等天然树根系统的全三维(3D)模型是通过垂直拔地从地面提取的,通过摄影测量重建,然后作为节点和根分支段进行骨架化。完整的3D模型和骨架模型的结合分析可以详细检查基本的体积特性和建筑参数的量化。根段可分为干根、主侧根和残根3大类,但主干、主侧根与残侧根在分枝和直径分布上存在显著差异。一般来说,分支角随着分支序列的增加而减小。靠近树干的侧根伸展明显,而靠近末端的侧根大致平行于母根生长。就枝长而言,根在树干附近分叉的频率更高,后来长得更长。局部厚度分析证实,树干附近的根径衰减速率高于其余侧根,而分支节点上的总横截面积基本保持保守。分支角直方图、分支长度直方图和分支厚度梯度直方图可以分别用对数正态分布和指数分布来描述。这项独特的研究提供了表征机械重要结构根的数据,这可能有助于将根结构与根结构的力学行为联系起来。
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引用次数: 0
Proposing a new sustainable approach for sand improvement using biologically-derived calcium phosphate cement 提出了一种新的可持续的方法来改善沙子使用生物衍生的磷酸钙水泥
Pub Date : 2024-08-27 DOI: 10.1016/j.bgtech.2024.100135
Sivakumar Gowthaman , Yuta Kumamoto , Kazunori Nakashima , Chikara Takano , Satoru Kawasaki
Bio-mediated soil improvement methods keep on gaining the attention of geotechnical engineers and researchers globally due to their nature-based elegance and eco-friendliness. Most prevalent bio-mediated soil improvement methods include microbially induced carbonate precipitation (MICP) and enzyme-induced carbonate precipitation (EICP). During their processes, the bacteria/free urease hydrolyzes the urea into ammonium and carbonic acid, which is accompanied by a considerable increase of alkalinity (about pH 9.0). The major problem associated with the above techniques is the release of gaseous ammonia that is extremely detrimental. Therefore, this study aims to propose a new sustainable approach involving lactic acid bacteria to facilitate the calcium phosphate mineralization for the strengthening of sand matrix. The major objectives of this investigation are: (i) to evaluate the urease activity of the lactic acid bacteria under different temperatures, pH conditions and additions of metal ions, (ii) to assess the treated sand matrix, (iii) to perform cost analysis. The outcomes indicated that Limosilactobacillus sp. could effectively facilitate the urea hydrolysis, hence increasing the pH from acidic to neutral and providing a desirable environment for the calcium phosphate to mineralize within the voids of the sand. The addition of 0.01 % Ni2+ in culture media was found to enhance the urease activity by 38.8 % and compressive strength over 40 %. A combined formation of amorphous- and whisker-like precipitates could bridge a larger area at particle-particle contact points, thereby faciliating a strong force-network in sand matrix. The mineralized calcium phosphate compound was found to be brushite. The cost herein for producing 1 L treatment solution was estimated to be about 2.5-folds and 11.8-folds lower compared to that of MICP and EICP treatment solutions, respectively. Moreover, since the treatment pH could potentially be regulated between acidic-neural range, it would greatly control the release of gaseous ammonia. With several environmental and economical benefits, the study has disclosed a new sustainable direction for sand improvement via the use of lactic acid bacteria.
生物介导的土壤改良方法以其自然优雅和生态友好的特点,不断受到全球岩土工程师和研究人员的关注。最流行的生物介导土壤改良方法包括微生物诱导碳酸盐沉淀(MICP)和酶诱导碳酸盐沉淀(EICP)。在此过程中,细菌/游离脲酶将尿素水解成铵和碳酸,同时碱度显著升高(pH约为9.0)。与上述技术相关的主要问题是气态氨的释放,这是极其有害的。因此,本研究旨在提出一种利用乳酸菌促进磷酸钙矿化以强化砂基质的可持续途径。本研究的主要目的是:(i)评估乳酸菌在不同温度、pH条件和金属离子添加下的脲酶活性,(ii)评估处理后的砂基质,(iii)进行成本分析。结果表明,limmosilactobacillus sp.能够有效地促进尿素水解,从而将pH从酸性提高到中性,为磷酸钙在砂土空隙内矿化提供了理想的环境。在培养基中添加0.01%的Ni2+,脲酶活性提高38.8%,抗压强度提高40%以上。非晶相和晶须相的结合形成可以在颗粒-颗粒接触点上桥接更大的区域,从而促进砂基质中强力网络的形成。矿化磷酸钙化合物被发现是刷石。与MICP和EICP处理液相比,生产1 L处理液的成本估计分别降低了约2.5倍和11.8倍。此外,由于处理pH可以在酸性-神经范围内调节,因此可以大大控制气态氨的释放。该研究具有良好的环境效益和经济效益,为利用乳酸菌对砂土进行可持续改良开辟了新的方向。
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引用次数: 0
Effect of humic acid on soil solidification by enzyme induced carbonate precipitation 腐植酸对酶促碳酸盐沉淀固化土壤的影响
Pub Date : 2024-08-12 DOI: 10.1016/j.bgtech.2024.100133
Meiqi Chen , Aoi Ichinohe , Kazunori Nakashima , Chikara Takano , Sivakumar Gowthaman , Lutfian R. Daryono , Satoru Kawasaki
Enzyme induced carbonate precipitation (EICP) is a promising technique in the field of biocementation due to its efficiency and controllability. Although many studies have proved its reliability in different environment, little attention has been paid to the influence of humic substances on the EICP. Humic substances cover most of the surface soil across the world land with vegetation, which varies according to the density of vegetation and climate. To understand the compatibility of this technique to distinct problematic soils, it is important to figure out how humic substances could affect the carbonate precipitation process induced by urease enzyme. Therefore, this study aims to investigate the effects of humic acid (HA), one type of humic substance, on the soil solidification through EICP. For this purpose, HA was added to natural soil with varying addition amounts (0%, 1%, 2%, 4%, 8%, 16%) in soil column solidification tests. The results found that the cementation effectiveness was enhanced by a small amount of HA addition (<4%), while an addition up to 8% greatly inhibited the formation of calcium carbonate. At the same time, soil samples were buffered by HA in a weak acidic condition, thus preventing the emission of undesirable by-product ammonia in the ureolysis process. Therefore, this study makes a contribution to research on enzymatic biocementation by demonstrating the effects of HA on the cementation effectiveness of EICP technique.
酶诱导碳酸盐沉淀(EICP)因其高效、可控性而成为生物胶结领域的一种很有前途的技术。虽然许多研究已经证明了其在不同环境下的可靠性,但对腐殖质物质对EICP的影响却很少关注。腐殖质物质覆盖了世界上大部分有植被的地表土壤,根据植被密度和气候的不同而不同。为了了解该技术对不同问题土壤的兼容性,重要的是要弄清楚腐殖质物质如何影响脲酶诱导的碳酸盐沉淀过程。因此,本研究旨在通过EICP研究腐植酸(HA)对土壤固化的影响。为此,在土柱固化试验中,将HA以不同的添加量(0%、1%、2%、4%、8%、16%)添加到天然土中。结果发现,少量HA(4%)的添加量可以增强胶结效果,而8%的添加量则可以极大地抑制碳酸钙的形成。同时,在弱酸性条件下,土壤样品被HA缓冲,从而防止了尿素解过程中不良副产物氨的排放。因此,本研究通过证明HA对EICP技术固接效果的影响,为酶促生物胶结研究做出了贡献。
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Biogeotechnics
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