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Development and optimization of biomimetic-chemically induced carbonate precipitation: A review of recent research 仿生化学诱导碳酸盐沉淀的开发与优化:最新研究综述
Pub Date : 2024-06-03 DOI: 10.1016/j.bgtech.2024.100110
Yu Diao , Jitao Bai , Gang Zheng , Qingsong Hu , Pengjin Li , Xuanqi Liu , Wendi Hu , Jianyou Huang
With further investigation on biomineralization, biomimetic mineralization has been proposed in imitation of microorganism behavior, in which the mechanism of biomineralization is utilized for the control of the crystal growth to synthesize inorganic materials with special structures and superior physical-chemical properties. This review summarizes the recent advances in biomimetic-chemically induced carbonate precipitation (BCICP). BCICP is a biomimetic mineralization process induced by calcium carbonate crystal modifiers, which directly regulates the metathesis reaction of calcium salts with carbonates in soils to improve the soil properties. Several crystal modifiers for BCICP, including the aspartic acid (organic), the boric acid (inorganic), and the polyacrylic acid (polymer), are reviewed, and the biomimetic mineralization mechanism is introduced. In addition, current findings about BCICP in cementitious materials, soil reinforcement, dust suppression, as well as other fields are discussed, aiming to give deeper insights into the further development and application of BCICP.
随着对生物矿化的进一步研究,人们提出了模仿微生物行为的生物仿生矿化,即利用生物矿化的机制控制晶体生长,从而合成具有特殊结构和优异物理化学性能的无机材料。本综述总结了生物仿生-化学诱导碳酸盐沉淀(BCICP)的最新进展。BCICP 是碳酸钙晶体改性剂诱导的生物模拟矿化过程,它直接调节土壤中钙盐与碳酸盐的偏析反应,从而改善土壤性质。本文综述了 BCICP 的几种晶体改性剂,包括天冬氨酸(有机)、硼酸(无机)和聚丙烯酸(聚合物),并介绍了其生物仿生矿化机制。此外,还讨论了目前有关 BCICP 在胶凝材料、土壤加固、抑尘以及其他领域的研究成果,旨在为 BCICP 的进一步开发和应用提供更深入的见解。
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引用次数: 0
Role of root morphological and architectural traits: Insights into root-inspired anchorage and foundation systems 根系形态和结构特征的作用:根系锚固和地基系统的启示
Pub Date : 2024-05-29 DOI: 10.1016/j.bgtech.2024.100107
Wengang Zhang , Ruijie Huang , Jiaying Xiang , Ningning Zhang , Matteo Oryem Ciantia , Leilei Liu , Jian Yin , Changbing Qin
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引用次数: 0
Feasibility study of enhancing enzyme-induced carbonate precipitation with eggshell waste for sand solidification 利用蛋壳废料加强酶诱导碳酸盐沉淀用于固沙的可行性研究
Pub Date : 2024-05-28 DOI: 10.1016/j.bgtech.2024.100108
Zhen Yan , Kazunori Nakashima , Chikara Takano , Satoru Kawasaki

Utilizing Enzyme-Induced Calcium Carbonate Precipitation (EICP) reinforcement technology has emerged as an innovative approach for soil improvement. In this study, kitchen waste eggshell powder was used as an additive material for EICP. The high external surface area and affinity for calcium ions of eggshell powder, which render it a suitable nucleation site for calcium carbonate precipitation. Experimental results demonstrate that the incorporation of eggshell powder, by increasing the number of nucleation sites and promoting calcium carbonate precipitation, reduces the inhibition of enzyme products, modulates the precipitation pattern of calcium carbonate, improves particle size distribution, and consequently significantly enhances the unconfined compressive strength of the samples. Furthermore, a neutral pH is achieved within the reaction system without the addition of any acid, thus preventing significant ammonia emissions. This underscores the potential of kitchen waste eggshells for recycling in biocement applications.

利用酶诱导碳酸钙沉淀(EICP)加固技术已成为土壤改良的一种创新方法。在这项研究中,厨余蛋壳粉被用作 EICP 的添加材料。蛋壳粉的高外表面积和对钙离子的亲和力使其成为碳酸钙沉淀的合适成核场所。实验结果表明,蛋壳粉的加入通过增加成核点的数量和促进碳酸钙的沉淀,减少了酶产物的抑制作用,调节了碳酸钙的沉淀模式,改善了粒度分布,从而显著提高了样品的无压抗压强度。此外,在反应系统中无需添加任何酸即可达到中性 pH 值,从而避免了大量氨的排放。这凸显了厨房废弃蛋壳在生物水泥应用中的回收潜力。
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引用次数: 0
Evaluating the performance and durability of concrete paving blocks enhanced by bio-cement posttreatment 评估经生物水泥后处理的混凝土砌块的性能和耐用性
Pub Date : 2024-05-27 DOI: 10.1016/j.bgtech.2024.100103
Navaratnam Rathivarman , Sivakumar Yutharshan , Alakenthiran Kabishangar , Vignarajah Janani , Sivakumar Gowthaman , Thiloththama Hiranya Kumari Nawarathna , Meiqi Chen , Satoru Kawasaki
Concrete pavement often experiences accelerated deterioration due to water and chemical ingress through micro-cracks and surface voids. Particularly, the ingress of aggressive agents into the concrete matrix results in irreversible changes and deterioration on its endurance. Numerous studies unveiled that hydrophobic surface protection could be an inexpensive and effective way of enhancing the durability of concrete. This research work aims to assess the feasibility of bio-cement posttreatment for facilitating hydrophobic surface protection, thus enhancing the performance and durability of concrete blocks. Enzyme induced carbonate precipitation (EICP) is one of the promising bio-cement methods. Concrete blocks casted in four different grades were subjected to EICP treatment with different treatment schemes and recipes of cementation media. The treated blocks were tested for water absorption, ultrasonic pulse velocity (UPV) measurements, unconfined compressive strength (UCS), thermal performance, and scanning electron microscopy (SEM). The results indicated that the concrete blocks subjected to EICP posttreatment showed over a 55% reduction in water absorption, a 15% higher UCS and a 6.7% higher UPV when compared with control blocks. The SEM analysis suggested that the EICP posttreatment could enhance the durability of concrete paving blocks by enabling a layer of calcite on the surface and by plugging the transport pore channels of the concrete. Although most of the posttreatment strategies investigated herein were found to be operative, a better response was seen in the posttreatment by spraying scheme with 0.5 mol/L cementation media (CM). With the successful demonstration, the EICP treatment prior to the use of concrete blocks can be recommended to the pavement construction industry.
由于水和化学物质通过微裂缝和表面空隙渗入,混凝土路面经常会加速老化。特别是侵蚀性介质进入混凝土基体后,会导致其发生不可逆转的变化,并使其耐久性下降。大量研究表明,疏水表面保护是提高混凝土耐久性的一种廉价而有效的方法。这项研究工作旨在评估生物水泥后处理的可行性,以促进疏水表面保护,从而提高混凝土砌块的性能和耐久性。酶诱导碳酸盐沉淀法(EICP)是一种很有前景的生物水泥方法。采用不同的处理方案和胶结介质配方,对四种不同等级的混凝土砌块进行了 EICP 处理。对处理后的砌块进行了吸水率、超声波脉冲速度(UPV)测量、无侧限抗压强度(UCS)、热性能和扫描电子显微镜(SEM)测试。结果表明,与对照砌块相比,经过 EICP 后处理的混凝土砌块吸水率降低了 55%,UCS 提高了 15%,UPV 提高了 6.7%。扫描电镜分析表明,EICP 后处理可在混凝土表面形成方解石层,并堵塞混凝土的传输孔道,从而提高混凝土砌块的耐久性。尽管本文所研究的大多数后处理策略都具有可操作性,但通过喷洒 0.5 mol/L 固结介质(CM)进行后处理的效果更好。通过这次成功的示范,可以向路面建筑行业推荐在使用混凝土砌块之前进行 EICP 处理。
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引用次数: 0
Comparison of the efficiency of traditional MICP and two-step MICP method for immobilizing heavy metals in aquatic environments 传统MICP法与两步MICP法固定化水中重金属的效果比较
Pub Date : 2024-05-25 DOI: 10.1016/j.bgtech.2024.100106
Xiaosong Huang , Rongjun Zhang , Junjie Zheng
The application of the microbially induced carbonate precipitation (MICP) method for remediating heavy metals (i.e., HMs) has recently garnered significant attention. Nevertheless, the inhibition of urease activity by toxic Cd2+, Pb2+, Zn2+, and Cu2+ poses a challenge for MICP-based remediation of HMs contamination. This study: (1) first performed the traditional MICP tests (in which the bacterial solution, urea solution, and HMs were mixed simultaneously), and investigated the toxic effect of HMs on the urease activity and the immobilization efficiency, (2) analyzed the toxicity and immobilization mechanism during the MICP process by combining the simulation and XRD tests, (3) conducted the two-step MICP tests (which initially mixed the bacterial solution and urea solution to promote urea hydrolysis, then added the HMs solutions for HMs precipitation) to improve the immobilization efficiency. The tube experiments and simulations were investigated in the HMs concentration range from 1 to 10 mmol/L. Indicators including ammonium concentration, HMs concentrations, and pH were measured/recorded during the tests. The results show that soluble HMs exhibit a concentration-dependent inhibition of urea hydrolysis during the traditional MICP process, resulting in a decreasing immobilization efficiency. The two-step MICP method can effectively immobilize almost the Cd2+ and Zn2+ when the initial urea hydrolysis period exceeds 1–2 h. In addition, a high immobilization rate of over 90% can be achieved for Cu-contaminated solutions at the optimal first-step reaction time. Compared with the traditional MICP procedure, the effective two-step MICP method exhibits more promising application prospects for the immobilization of soluble HMs in aquatic environments.
应用微生物诱导碳酸盐沉淀(MICP)方法修复重金属(即HMs)最近引起了极大的关注。然而,毒性Cd2+、Pb2+、Zn2+和Cu2+对脲酶活性的抑制对基于micp的HMs污染修复提出了挑战。本研究:(1)首先进行传统的MICP试验(细菌溶液、尿素溶液和HMs同时混合),考察HMs对脲酶活性和固定化效率的毒性作用;(2)通过模拟和XRD试验相结合,分析MICP过程中的毒性和固定化机制;(3)进行两步MICP试验(首先将细菌溶液和尿素溶液混合,促进尿素水解;然后加入HMs溶液进行HMs沉淀),提高固定化效率。在1 ~ 10 mmol/L的HMs浓度范围内进行了试管实验和模拟。在试验期间测量/记录铵浓度、HMs浓度和pH等指标。结果表明,在传统的MICP工艺中,可溶性HMs对尿素水解的抑制作用呈浓度依赖性,导致固定化效率降低。当初始尿素水解时间超过1 ~ 2 h时,两步MICP法几乎可以有效地固定Cd2+和Zn2+,并且在最佳的第一步反应时间下,cu污染溶液的固定率可以达到90%以上。与传统的MICP方法相比,有效的两步MICP方法在水生环境中固定化可溶性HMs具有更广阔的应用前景。
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引用次数: 0
Uniformity of microbial injection for reinforcing saturated calcareous sand: A multi-test approach 用于加固饱和石灰质砂的微生物注入的均匀性:多重测试方法
Pub Date : 2024-05-23 DOI: 10.1016/j.bgtech.2024.100105
Xinlei Zhang , Yue Sun , Yumin Chen , Lu Liu , Wenwen Li , Yi Han
The mineralization process of microbial-induced calcium carbonate precipitation (MICP) is influenced by many factors, and the uniformity of the calcium carbonate precipitation has become the main focus and challenge for MICP technology. In this study, the uniformity of the saturated calcareous sand treated with MICP was investigated through one-dimensional calcareous sand column tests and model tests. The coefficient of variation was employed in one-dimensional sand column tests to investigate the impact of injection rate, cementation solution concentration, and number of injection cycles on the uniformity of the MICP treatment. Additionally, model tests were conducted to investigate the impact of injection pressure and methods on the treatment range and uniformity under three-dimensional seepage conditions. Test results demonstrate that the reinforcement strength and uniformity are significantly influenced by the injection rate of the cementation solution, with a rate of 3 mL/min, yielding a favorable treatment effect. Excessive concentration of the cementation solution can lead to significant non-uniformity and a reduction in the compressive strength of MICP-treated samples. Conversely, excessively low concentrations may result in decreased bonding efficiency. Among the four considered concentrations, 0.5 mol/L and 1 mol/L exhibit superior reinforcing effects. The morphological development of calcareous sandy foundation reinforcement is associated with the spatial distribution pattern of the bacterial solution, exhibiting a relatively larger reinforcement area in proximity to the lower region of the model and a gradually decreasing range towards the upper part. Under three-dimensional seepage conditions, in addition to the non-uniform radial cementation along the injection pipe, there is also vertical heterogeneity of cementation along the length of the injection pipe due to gravitational effects, resulting in preferential deposition of calcium carbonate at the lower section. The application of injection pressure and a double-pipe circulation injection method can mitigate the accumulation of bacterial solution and cementation solution at the bottom, thereby improving the reinforcement range and uniformity.
微生物诱导碳酸钙沉淀(MICP)矿化过程受多种因素影响,碳酸钙沉淀的均匀性已成为MICP技术的主要焦点和挑战。通过一维钙质砂柱试验和模型试验,研究了饱和钙质砂经MICP处理后的均匀性。利用变异系数进行一维砂柱试验,考察注入速率、胶结液浓度和注入循环次数对MICP处理均匀性的影响。此外,通过模型试验研究了三维渗流条件下注入压力和注入方式对处理范围和均匀性的影响。试验结果表明,胶结液的注入速度对增强强度和均匀性有显著影响,注入速度为3 mL/min,处理效果良好。胶结溶液浓度过高会导致显著的不均匀性,并降低micp处理样品的抗压强度。反之,过低的浓度可能导致键合效率下降。在4个考虑浓度中,0.5 mol/L和1 mol/L表现出更强的强化作用。钙质砂基加固的形态发育与细菌溶液的空间分布格局有关,接近模型下部的加固面积相对较大,靠近模型上部的加固面积逐渐减小。在三维渗流条件下,除了沿注入管方向的径向胶结不均匀外,由于重力作用,沿注入管长度方向的胶结在垂直方向上也存在非均匀性,导致碳酸钙在下部优先沉积。施加注射压力和采用双管循环注射方式,可以减轻底部细菌溶液和胶结液的积累,从而提高加固范围和均匀性。
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引用次数: 0
Fracture sealing based on microbially induced carbonate precipitation and its engineering applications: A review 基于微生物诱导碳酸盐沉淀的断裂密封及其工程应用:综述
Pub Date : 2024-05-05 DOI: 10.1016/j.bgtech.2024.100100

In this review, the development and application of microbially induced carbonate precipitation (MICP) technology for the sealing of underground engineering fractures are discussed in detail. The importance of sealing micro-fractures in an environmentally friendly and efficient manner is emphasized, and the potential of the MICP method in controlling pore and fracture seepage is highlighted. The fundamental mechanisms, key influencing factors, numerical models, and applications of the MICP in the fields of geological CO2 storage and oil resources development are comprehensively summarized in the paper. At the same time, the limitations of the existing research and the future research directions are discussed, especially in terms of improving the processing efficiency, environmental impacts, and cost considerations. Overall, the development of MICP technology provides a new environmentally friendly reinforcement method for geotechnical engineering and is expected to play a key role in the future development of underground space engineering.

本综述详细讨论了用于地下工程裂缝封堵的微生物诱导碳酸盐沉淀(MICP)技术的开发和应用。文章强调了以环保、高效的方式封堵微裂缝的重要性,并着重介绍了微生物诱导碳酸盐沉淀法在控制孔隙和裂缝渗流方面的潜力。论文全面总结了 MICP 的基本机理、关键影响因素、数值模型以及在二氧化碳地质封存和石油资源开发领域的应用。同时,还讨论了现有研究的局限性和未来的研究方向,特别是在提高处理效率、环境影响和成本考虑等方面。总之,MICP 技术的发展为岩土工程提供了一种新的环境友好型加固方法,有望在地下空间工程的未来发展中发挥关键作用。
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引用次数: 0
Endeavours to achieve sustainable marine infrastructures: A new “window” for the application of biomineralization in marine engineering 努力实现可持续海洋基础设施:在海洋工程中应用生物矿化的新 "窗口"
Pub Date : 2024-05-03 DOI: 10.1016/j.bgtech.2024.100098
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引用次数: 0
Effects of microbially induced calcite precipitation on static liquefaction behavior of a gold tailings sand 微生物诱导方解石沉淀对金尾矿砂静态液化行为的影响
Pub Date : 2024-04-27 DOI: 10.1016/j.bgtech.2024.100097
Hamed Behzadipour , Abouzar Sadrekarimi

Loose tailings are susceptible to static liquefaction during which they lose a substantial amount of their strength. This study examines a sustainable technique known as Microbially-Induced Calcite Precipitation (MICP) to improve the static liquefaction resistance of gold mine silty sand tailings. These materials were enriched with Sporosarcina pasteurii, consolidated in a direct simple shearing apparatus, and subjected to several injections of a cementation solution. Calcified tailings were then sheared under constant-volume and constant vertical stress conditions to evaluate their undrained and drained shearing behaviors. Results showed that bio-mineralization can prevent the occurrence of static liquefaction in tailings by reducing their contraction tendency. This is demonstrated by the strong strain-hardening behaviors of the treated tailings specimens compared to the strain-softening and undrained strength loss in specimens of the untreated tailings. Substantial increases in the tailings undrained and drained shear strengths (by up to 30 - 50 kPa), improvements (by up to 5 MPa) in their tangent moduli, and more than 5° rise in their friction angles are observed in the direct simple shear tests following MICP-treatment. The critical state line of tailings is also found to be steeper and shifted to denser void ratios following MICP treatment. These changes reduce liquefaction susceptibility of tailings and enhance their resistance against static liquefaction. Post-treatment acid dissolution further indicates that CaCO3 contents of about 4% to 11% precipitated in the treated specimens. This amount decreases with increasing specimens void ratio. Changes in the microstructural fabric of the cemented tailings particles are also characterized using scanning electron microscopic (SEM) images and X-ray diffraction (XRD) analyses.

松散的尾矿很容易发生静态液化,在液化过程中会损失大量强度。本研究探讨了一种称为微生物诱导方解石沉淀(MICP)的可持续技术,以提高金矿淤泥砂尾矿的抗静态液化能力。这些材料富含巴氏芽孢杆菌,在直接简易剪切设备中固结,并多次注入胶结溶液。然后在恒定体积和恒定垂直应力条件下对钙化尾矿进行剪切,以评估其排水和排水剪切行为。结果表明,生物矿化可以通过降低尾矿的收缩趋势来防止其发生静态液化。与未经处理的尾矿试样的应变软化和排水强度损失相比,经过处理的尾矿试样具有很强的应变硬化行为,这就证明了这一点。在经过 MICP 处理后进行的直接简单剪切试验中,可以观察到尾矿的排水和排水剪切强度大幅提高(最多可提高 30 - 50 kPa),切线模量提高(最多可提高 5 MPa),摩擦角上升超过 5°。经 MICP 处理后,还发现尾矿的临界状态线更加陡峭,并向更密集的空隙率转移。这些变化降低了尾矿的液化敏感性,增强了其抗静态液化的能力。处理后的酸溶解进一步表明,处理后的试样中析出了约 4% 至 11% 的 CaCO3。这一含量随着试样空隙率的增加而减少。此外,还利用扫描电子显微镜(SEM)图像和 X 射线衍射(XRD)分析确定了胶结尾矿颗粒微观结构的变化特征。
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引用次数: 0
Does biochar mitigate rainfall-induced soil erosion? A review and meta-analysis 生物炭能减轻降雨引起的土壤侵蚀吗?综述与荟萃分析
Pub Date : 2024-04-26 DOI: 10.1016/j.bgtech.2024.100096
Yu Lu , Kai Gu , Bin Shi , Qiyou Zhou

Biochar has emerged as a promising soil amendment for improving soil structure. Yet, its impact on rainfall-induced soil erosion varies across individual studies. To address this gap, we conducted a statistical meta-analysis of 174 paired comparisons from 45 published studies to integratedly evaluate the impacts of biochar on rainfall-induced soil erosion through biochar and soil properties, as well as experimental conditions. Overall, biochar significantly reduced soil erosion by 27.86%. The response ratio (lnRR) of biochar-induced soil erosion exhibited significant variability across different subgroups. Concerning biochar properties, a more favorable influence was observed in other sources biochar (e.g., manure and sewage sludge biochar) compared to wood based and crop waste biochar, and those produced at lower pyrolysis temperatures (< 500 °C). Increasing biochar dosage was not consistently effective. The optimal range was 0.8%–2%, resulting in a 36.07% reduction in soil erosion. Regarding the soil properties, a higher sand/clay ratio of soil significantly enhanced the performance of biochar (p < 0.0001). Specifically, an insignificant effect was observed in fine-grained soils, whereas the highest reduction of 52.97% was noted in coarse-grained soils. Moreover, long-term field experiments induced greater reductions in soil erosion with biochar (35.30%) compared to short-term laboratory studies (29.62% and 12.59%). This meta-analysis demonstrates that biochar, as a potential soil amendment, could effectively mitigate rainfall-induced soil erosion by considering a combination of soil properties along with specific biochar properties.

生物炭已成为一种很有前景的土壤改良剂,可改善土壤结构。然而,生物炭对降雨引起的土壤侵蚀的影响在不同的研究中存在差异。为了弥补这一不足,我们对 45 项已发表研究中的 174 项配对比较进行了统计荟萃分析,通过生物炭和土壤特性以及实验条件综合评估了生物炭对降雨引起的土壤侵蚀的影响。总体而言,生物炭明显减少了 27.86% 的土壤侵蚀。生物炭引起的土壤侵蚀的响应比(lnRR)在不同分组中表现出显著的差异性。在生物炭特性方面,与木质生物炭和农作物废料生物炭相比,其他来源的生物炭(如粪便和污水污泥生物炭)以及在较低热解温度(< 500 °C)下生产的生物炭具有更有利的影响。增加生物炭用量的效果并不一致。最佳范围为 0.8%-2%,结果土壤侵蚀减少了 36.07%。在土壤特性方面,土壤中砂/粘土的比例越高,生物炭的效果越明显(p <0.0001)。具体而言,细粒度土壤的影响不明显,而粗粒度土壤的影响最大,减少了 52.97%。此外,与短期实验室研究(29.62% 和 12.59%)相比,长期田间试验诱导生物炭更大程度地减少了土壤侵蚀(35.30%)。这项荟萃分析表明,生物炭作为一种潜在的土壤改良剂,可通过综合考虑土壤特性和生物炭的具体特性,有效减轻降雨引起的土壤侵蚀。
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