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Saturated permeability and water retention capacity in biochar-methanotrophs-clay for new landfill cover system 新型垃圾填埋场覆盖系统中生物炭-甲烷-粘土的饱和渗透性和保水能力
Pub Date : 2024-04-09 DOI: 10.1016/j.bgtech.2024.100089
Wenjing Sun , Gaoge Sun , Shuyun Zhang

A new landfill cover system, biochar-methanotrophs-clay (BMC) cover is recommended for reducing methane emissions at landfills. It also contributes to decreasing soil permeability and improving soil water retention in a long time, due to highly porous structure of biochar and the growth metabolism of methanotrophs. To determine the effects of biochar content, oxidation aging times and methane-filled days on hydraulic properties, a total of 60 groups of experiments were conducted. The saturated hydraulic conductivity (ksat) was obtained by flexible wall permeameter with controllable hydraulic head pressure. The results showed that the ksat of BMC increased with increasing biochar content and oxidation aging times, while decreased with adding methane-filled days. The soil-water characteristic curves (SWCCs) were obtained with soil suction measured by the filter paper method. The results indicated the water retention capacity of MBC reduced with increasing oxidation aging times but increased with adding methane-filled days. Detected by mercury intrusion porosimetry (MIP), fourier transform infrared spectroscopy (FTIR) and scanning electron microscope (SEM), the differences displayed the changes of pore structures and extracellular polymeric substances (EPS). The oxidation aging of biochar increased the volume of pores, resulting in the increased ksat and the decreased water retention capacity. However, the growing of methanotrophs decreased the volume of pores, resulting in the ksat decreased and the water retention capacity increased due to EPS. No matter how many times the oxidation aging process was experienced, the BMC with longer methane-filled days exhibited relatively lower ksat and better water retention capacity. This implied a more stable barrier capacity to reduce water infiltration in the long term. By combing a series of macro and micro experiments, this paper provides theoretical guidance for the application of biochar-methanotroph-clay mixture to landfill covers.

为减少垃圾填埋场的甲烷排放,建议采用一种新的垃圾填埋场覆盖系统,即生物炭-甲烷营养土(BMC)覆盖系统。由于生物炭的高孔隙结构和甲烷养分菌的生长代谢,它还有助于降低土壤的渗透性和提高土壤的长期保水性。为了确定生物炭含量、氧化老化时间和甲烷填充天数对水力特性的影响,共进行了 60 组实验。饱和导水性(ksat)是通过可控水头压力下的柔性壁渗透仪获得的。结果表明,BMC 的 ksat 随生物炭含量和氧化老化时间的增加而增加,但随甲烷填充天数的增加而减少。通过滤纸法测量土壤吸力,获得了土壤水特征曲线(SWCC)。结果表明,随着氧化老化时间的增加,MBC 的保水能力降低,但随着甲烷填充天数的增加,保水能力提高。通过汞侵入孔隙测定法(MIP)、傅立叶变换红外光谱法(FTIR)和扫描电子显微镜(SEM)检测,差异显示了孔隙结构和胞外聚合物质(EPS)的变化。生物炭的氧化老化增加了孔隙体积,导致 ksat 增加和保水能力下降。然而,甲烷滋养菌的生长减少了孔隙体积,导致 ksat 下降,而 EPS 则增加了保水能力。无论经历多少次氧化老化过程,甲烷填充天数较长的 BMC 的 ksat 都相对较低,保水能力较强。这意味着在长期减少水渗入方面具有更稳定的阻隔能力。通过结合一系列宏观和微观实验,本文为生物炭-甲烷-粘土混合物在垃圾填埋场覆盖层中的应用提供了理论指导。
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引用次数: 0
Experimental study on reinforcement of bionic grouser of deep-sea mining vehicle 深海采矿器仿生栅格加固实验研究
Pub Date : 2024-04-09 DOI: 10.1016/j.bgtech.2024.100088
Jiancheng Liu , Xuelin Liu , Xiuzhan Zhang , Xuguang Chen , Hao Li , Lubao Luan , Cong Ding , Xingzheng Gao

To fulfill the operational demands of deep-sea tracked mining vehicles traversing soft seabed substrates, an evaluation of the characteristics of these substrates was conducted, drawing a comparison with the land swamp black soil found in the buffalo's habitat. Employing the principles of biomimicry, two distinct types of bionic grouser were devised, replicating the configuration of the buffalo's hooves in both the horizontal and vertical planes. Utilizing self-constructed testing platforms, exhaustive examinations of the reinforcement efficacy of these bionic track grousers were undertaken, spanning from single-grouser to multi-grouser configurations and encompassing the entire track assembly. The findings unequivocally demonstrate a pronounced and consistent enhancement in traction force for both types of bionic grousers. Notably, the W-shaped bionic grouser, mimicking the horizontal contour of the buffalo's hoof, exhibits the most substantial increase in traction force. The maximum enhancement in traction force for individual bionic grouser exceeds 30%, while the overall track achieves an increase of over 19%. This research provides a valuable reference and establishes a foundational framework for the design of equipment tailored for the locomotion of deep-sea tracked mining vehicles across soft substrates.

为满足深海履带式采矿车在松软海底基质中穿越的作业要求,对这些基质的特性进行了评估,并与水牛栖息地的陆地沼泽黑土进行了比较。利用仿生学原理,设计了两种不同类型的仿生蹄铁,在水平和垂直平面上复制了水牛蹄子的构造。利用自建的测试平台,对这些仿生履带蹄铁的加固效果进行了详尽的测试,测试范围包括单蹄铁到多蹄铁配置以及整个履带组件。研究结果明确显示,两种类型的仿生履带都能显著、持续地增强牵引力。值得注意的是,模仿水牛蹄部水平轮廓的 W 型仿生蹄铁的牵引力增幅最大。单个仿生蹄铁的牵引力最大增幅超过 30%,而整个履带的牵引力增幅超过 19%。这项研究为设计专门用于深海履带式采矿车辆在软基底上运动的设备提供了宝贵的参考,并建立了一个基础框架。
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引用次数: 0
Factors affecting the effectiveness of biocementation of soil 影响土壤生物固化效果的因素
Pub Date : 2024-04-02 DOI: 10.1016/j.bgtech.2024.100087
Hanjiang Lai , Xingzhi Ding , Mingjuan Cui , Junjie Zheng , Jian Chu , Zhibo Chen

Microbially or enzyme induced carbonate precipitation has emerged to be a new type of soil improvement method. However, it appears that the biocementation process is affected by many factors and a common understanding on the control factors on the biocement effect has not been reached. This paper attempts to identify the main factors that controlling the MICP or EICP effect through an in-depth discussion on the fundamentals of biocementation process. Similar to other cemented granular materials, biocemented soil is a structural soil composite consisting of soil skeleton and biocement force chain or biocement network. The strength and stiffness of the biocemented soil is controlled by the reinforcement effect of the biocement network on the soil skeleton or the interplay of the soil skeleton and precipitates. The contribution of the strength by soil skeleton is affected by the soil types and soil properties, while the contribution of the precipitates is through the distribution of the biocement network and the properties of the precipitates.

微生物或酶诱导碳酸盐沉淀已成为一种新型的土壤改良方法。然而,生物固结过程似乎受到很多因素的影响,对生物固结效果的控制因素尚未达成共识。本文试图通过深入探讨生物固结过程的基本原理,找出控制 MICP 或 EICP 效果的主要因素。与其他胶结颗粒材料类似,生物水泥土也是一种由土壤骨架和生物水泥力链或生物水泥网络组成的土壤结构复合材料。生物加固土的强度和刚度由生物水泥网络对土壤骨架的加固作用或土壤骨架与沉淀物的相互作用控制。土壤骨架对强度的贡献受土壤类型和土壤性质的影响,而沉淀物的贡献则通过生物水泥网络的分布和沉淀物的性质来实现。
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引用次数: 0
Experimental study on self-burrowing dual anchor soft probe 自生长双锚软探针实验研究
Pub Date : 2024-04-01 DOI: 10.1016/j.bgtech.2024.100086
Jia He, Hao Wang, Xin Huang, Fengyuan Yan

This study focuses on the development and testing of a bio-inspired self-burrowing dual anchor soft probe for potential geotechnical applications. Dual anchor refers to the form of movement in soils in which some bivalve molluscs adopted by alternately generating anchoring effects in the soil through shell expansion and fluid-filled feet. By mimicking this mechanism, this study used pneumatic artificial muscles as soft actuators and developed an autonomous burrowing probe. The structure and the performance of the actuators and the probe were investigated and optimized. The burrowing-out process of the dual anchor probe was not a simple upward movement. Instead, it rose in the inflation phase and slipped downward in the deflation phase. The difference between the two was a stride in one single step. In the sands with relative densities of 30%, 50%, and 80%, the total slips accounted for 18.8%, 19.6%, and 26.9% of the total upward movements, respectively. Thus, the entire movement process showed a reciprocating upward trend. The burrowing process could be divided into a restricted stage and a free stage according to whether shear failure occurs in the overlying soil layer. When the soil density was high, the initial stage of burrowing was in a restricted stage. The amount of rise and slip were at a low level and increased slowly as the number of cycles increased. When the burrowing process was in the free stage, the increase was basically stable at a high value and accompanied by small slips.

本研究的重点是开发和测试一种生物启发的自生长双锚软探针,用于潜在的岩土工程应用。双锚指的是一些双壳软体动物在土壤中的运动形式,它们通过壳的膨胀和充满液体的足交替在土壤中产生锚定效应。通过模仿这种机制,本研究使用气动人工肌肉作为软执行器,开发了一种自主钻探探头。研究并优化了致动器和探针的结构和性能。双锚探针的钻出过程并非简单的向上运动。相反,它在充气阶段上升,在放气阶段向下滑动。两者之间的差距是一步之遥。在相对密度为 30%、50% 和 80% 的沙地中,总下滑量分别占总上升运动量的 18.8%、19.6% 和 26.9%。因此,整个移动过程呈往复上升趋势。根据上覆土层是否发生剪切破坏,掘进过程可分为受限阶段和自由阶段。当土壤密度较高时,掘进的初始阶段处于受限阶段。上升量和滑移量处于较低水平,并随着循环次数的增加而缓慢增加。当掘进过程处于自由阶段时,上升量基本稳定在一个较高值,并伴有小的滑移。
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引用次数: 0
Feasibility of micro-organisms in soil bioremediation and dust control 微生物在土壤生物修复和粉尘控制中的可行性
Pub Date : 2024-03-28 DOI: 10.1016/j.bgtech.2024.100085
Erfan Ahmadzadeh , Sima Samadianfard , Yang Xiao , Vahab Toufigh

Detrimental impacts of dust caused by mine tailings have yielded to several studies on the efficiency of different soil stabilizers. Bacterial stabilization has been recognized as a reality within recent decades, where bacteria could get adhesion to the grains and stabilize the soil particles. However, these bacteria are prone to be destroyed while exposed to the normal environmental conditions. In this study, the effects of microcapsules containing two types of bacterial freeze-dried spores (B.Subtilis Natto LMG 19457 and B.ESH) have been investigated on the mine tailing stability in terms of two parts. The first part of the study is dedicated to the fabrication of microcapsules within the two bacteria and identification of the characteristics of these microcapsules to set the time of microcapsules break and release in the soil. The urea-formaldehyde microcapsules containing tung oil were synthesized using microencapsulation method and at the following, the bacterial spores of B.Subtilis Natto LMG 19457 and B.ESH which had the high durability and the capability to grow in the silicon oil, were added to the microcapsules. The microcapsules effect on MT specimens and the viability of encapsulated spores were determined. The characteristics of the capsules were analyzed by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR) and thermo-gravimetric thermal analysis (TGA). In the second part, wind tunnel tests were conducted to study the effects of microorganism stabilizers on mine tailings. The results indicated that the dust erosion reduced from 16% - using water as a stabilizer- to the 0.2% while using microcapsules containing B.Subtilis Natto LMG 19457 and 0.8% while using microcapsules containing ESH. The results showed the high efficiency of microcapsules containing bacteria in stabilizing the MTs. This phenomenon was proved by SEM imaging in which the voids were bounded significantly while using the bacteria.

矿山尾矿造成的粉尘有害影响促使人们对不同土壤稳定剂的效率进行了多项研究。近几十年来,细菌稳定剂已被认为是一种现实,细菌可以附着在颗粒上,稳定土壤颗粒。然而,这些细菌在正常环境条件下很容易被破坏。本研究分两部分研究了含有两种细菌冻干孢子(B.Subtilis Natto LMG 19457 和 B.ESH)的微胶囊对矿山尾矿稳定性的影响。研究的第一部分致力于在这两种细菌中制造微胶囊,并鉴定这些微胶囊的特性,以确定微胶囊在土壤中破裂和释放的时间。采用微胶囊法合成了含桐油的脲醛微胶囊,然后在微胶囊中加入了耐久性强且能在硅油中生长的纳豆 LMG 19457 和 ESH 细菌孢子。测定了微胶囊对 MT 试样的影响以及封装孢子的活力。通过扫描电子显微镜(SEM)、傅立叶变换红外光谱(FTIR)和热重力热分析(TGA)分析了胶囊的特性。第二部分是风洞试验,研究微生物稳定剂对矿山尾矿的影响。结果表明,使用含有 B.Subtilis Natto LMG 19457 的微胶囊时,粉尘侵蚀率从用水作为稳定剂时的 16% 降至 0.2%,使用含有 ESH 的微胶囊时降至 0.8%。结果表明,含有细菌的微胶囊在稳定 MT 方面具有很高的效率。扫描电镜成像证明了这一现象,在使用细菌的微胶囊中,空隙被明显束缚。
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引用次数: 0
Dancing with crystals: Bacterial functions and interactions in biomineralization 与水晶共舞细菌在生物矿化过程中的功能和相互作用
Pub Date : 2024-03-12 DOI: 10.1016/j.bgtech.2024.100084
Jinxuan Zhang , Yang Xiao , Hao Cui , Xiang He , Hanlong Liu
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引用次数: 0
A new bioslurry-induced restoration method via biomineralization for fragmented ceramic cultural relics 通过生物矿化作用对碎裂陶瓷文物进行生物泥浆诱导修复的新方法
Pub Date : 2024-03-05 DOI: 10.1016/j.bgtech.2024.100082
Yang Yang , Han Zhang , Liang Cheng , Lin Ye , Yuanjian Liu , Jinquan Shi , Jianwei Zhang , Yang Xiao , Hanlong Liu

This study presents a new restoration method for fragmented ceramic cultural relics using bioslurry-induced biocementation via a microbially induced calcium carbonate precipitation (MICP) process. Bioslurry is highly urease active calcium carbonate crystals, which serve as filling and cementitious material with newly induced calcite precipitation when supplying cementation solution (urea and calcium source). With the pre-filling of bioslurry and newly induced calcite crystals, the fragmented ceramic can be connected and the gap along the fracture surface can be sealed. Due to the high urease active bacteria cells embedded in bioslurry, the ceramic restoration can be completed in 24 h with the optimal concentration of cementation solution of 1.6 M. Taking the advantage of bonding effect gained from newly induced calcite precipitation, the tensile strength was improved up to 0.92 MPa through a customized tensile strength test. This is satisfactory to ensure the stability and integrity of fragmented ceramic after bioslurry-induced restoration. A demonstrative restoration has been completed on fragmented ceramics from Ming Dynasty. With the good bonding strength and high stability of bioslurry-induced calcite precipitation, the proposed bioslurry-induced restoration method contributes valuable insights to the conservation of ceramic cultural relics. Other prospective applications include the restoration of masonry relics and bone relics.

本研究通过微生物诱导碳酸钙沉淀(MICP)过程,提出了一种利用生物浆液诱导生物水泥化的新方法来修复碎裂的陶瓷文物。生物浆是具有高度脲酶活性的碳酸钙晶体,在提供胶结溶液(尿素和钙源)时,可作为填充和胶凝材料,与新诱导的方解石沉淀一起使用。有了生物浆和新诱导方解石晶体的预填充,破碎的陶瓷就可以连接起来,裂缝表面的缝隙也可以被封住。由于生物浆中蕴含高活性的尿素酶细菌细胞,在最佳固结液浓度为 1.6 M 的情况下,陶瓷修复可在 24 小时内完成。利用新诱导方解石析出的粘结效果,通过定制的抗拉强度测试,抗拉强度提高到 0.92 MPa。这对于确保生物浆液诱导修复后碎裂陶瓷的稳定性和完整性是令人满意的。明代碎陶瓷的示范修复工作已经完成。生物浆液诱导方解石析出具有良好的粘结强度和高稳定性,所提出的生物浆液诱导修复方法为陶瓷文物保护提供了宝贵的见解。其他应用前景还包括砖石文物和骨质文物的修复。
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引用次数: 0
Enhancing carbon neutrality: A perspective on the role of Microbially Induced Carbonate Precipitation (MICP) 加强碳中性:透视微生物诱导碳酸盐沉淀 (MICP) 的作用
Pub Date : 2024-03-05 DOI: 10.1016/j.bgtech.2024.100083
Chaolin Fang , Varenyam Achal

Microbially Induced Carbonate Precipitation (MICP) presents a promising avenue for sustainable carbon management, offering a rapid alternative to natural carbonate formation. This paper explores the potential of MICP, particularly through ureolysis, in carbon storage and greenhouse gas mitigation. Urease-producing bacteria play a key role by converting CO2 into calcium carbonate (CaCO3). These microbes thrive in various environments, from soils to construction sites, making MICP a versatile tool for Carbon Capture and Storage (CCS). This process not only results in the formation of solid carbonates but also effectively sequesters CO2, positioning MICP as a transformative approach for climate change mitigation. The article highlights MICP’s capacity to harness microbial activities for environmental benefits, emphasizing its importance in reducing atmospheric CO2 levels and contributing to a more sustainable future.

微生物诱导碳酸盐沉淀(MICP)为可持续碳管理提供了一条前景广阔的途径,可迅速替代天然碳酸盐的形成。本文探讨了微生物诱导碳酸盐沉淀(MICP),特别是通过尿素分解,在碳储存和温室气体减排方面的潜力。产生尿素酶的细菌通过将二氧化碳转化为碳酸钙(CaCO3)发挥着关键作用。这些微生物在从土壤到建筑工地的各种环境中繁衍生息,使 MICP 成为碳捕集与封存 (CCS) 的多功能工具。这一过程不仅能形成固体碳酸盐,还能有效封存二氧化碳,使 MICP 成为减缓气候变化的一种变革性方法。文章强调了 MICP 利用微生物活动为环境造福的能力,强调了它在降低大气二氧化碳含量和促进更可持续的未来方面的重要性。
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引用次数: 0
Cementor: A toolbox to generate bio-cemented soils with specific microstructures Cementor:生成具有特定微观结构的生物加固土壤的工具箱
Pub Date : 2024-03-04 DOI: 10.1016/j.bgtech.2024.100081
Aoxi Zhang , Anne-Catherine Dieudonné

Bio-cemented soils can exhibit various types of microstructure depending on the relative position of the carbonate crystals with respect to the host granular skeleton. Different microstructures can have different effects on the mechanical and hydraulic responses of the material, hence it is important to develop the capacity to model these microstructures. The discrete element method (DEM) is a powerful numerical method for studying the mechanical behaviour of granular materials considering grain-scale features. This paper presents a toolbox that can be used to generate 3D DEM samples of bio-cemented soils with specific microstructures. It provides the flexibility of modelling bio-cemented soils with precipitates in the form of contact cementing, grain bridging and coating, and combinations of these distribution patterns. The algorithm is described in detail in this paper, and the impact of the precipitated carbonates on the soil microstructure is evaluated. The results indicate that carbonates precipitated in different distribution patterns affect the soil microstructure differently, suggesting the importance of modelling the microstructure of bio-cemented soils.

根据碳酸盐晶体与主颗粒骨架的相对位置,生物胶结土壤可呈现出各种类型的微观结构。不同的微观结构会对材料的机械和水力响应产生不同的影响,因此开发模拟这些微观结构的能力非常重要。离散元素法(DEM)是一种功能强大的数值方法,可用于研究考虑到颗粒尺度特征的颗粒材料力学行为。本文介绍的工具箱可用于生成具有特定微观结构的生物加固土壤三维 DEM 样本。该工具箱可灵活建模具有接触胶结、晶粒桥接和包覆等沉淀物形式的生物胶结土壤,以及这些分布模式的组合。本文详细介绍了该算法,并评估了析出碳酸盐对土壤微观结构的影响。结果表明,以不同分布模式析出的碳酸盐对土壤微观结构的影响是不同的,这表明建立生物胶结土壤微观结构模型的重要性。
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引用次数: 0
Evaluation of plant growth and spacing effects on bioengineered slopes subjected to rainfall 评估降雨对生物工程斜坡植物生长和间距的影响
Pub Date : 2024-02-20 DOI: 10.1016/j.bgtech.2024.100080
Farshad Yazdani , Hamed Sadeghi , Pouya AliPanahi , Mostafa Gholami , Anthony Kwan Leung

Shallow landslides can be mitigated through the hydro-mechanical reinforcement provided by vegetation. Several critical parameters, such as plant spacing and plant age, play a significant role in influencing bioengineered slope stability facilitated by vegetation. However, the coupling of these effects on the stability of vegetated slope has been ignored. The objective of this study is to investigate the hydro-mechanical impact of vegetation growth and spacing on the stability of bioengineered slopes based on the predictions of a calibrated numerical model against field measurements. The impact of vegetation is investigated, with specific attention given to different plant spacing and growth stages, utilizing Schefflera arboricola. In the context of rainfall, it was observed that younger vegetation demonstrated more effective matric suction retention and recovery up to 25 kPa compared to the aged vegetation. Vegetation was revealed to substantially enhance the factor of safety up to 0.3 compared to the bare slope. Plant growth and reducing plant spacing increased the impact of root systems on both hydraulic and mechanical stability, primarily attributable to the influence of root cohesion rather than transpiration rates. The results revealed that the mechanical contribution to the factor of safety enhancement was raised from one-third to two-thirds because of the vegetation-induced cohesion within the growing rooted zone.

浅层滑坡可以通过植被提供的水力机械加固来缓解。植物间距和植株年龄等几个关键参数在植被促进生物工程边坡稳定性方面发挥着重要作用。然而,这些影响对植被边坡稳定性的耦合作用一直被忽视。本研究的目的是根据经校准的数值模型预测和实地测量结果,研究植被生长和间距对生物工程边坡稳定性的水力机械影响。研究利用 Schefflera arboricola 研究了植被的影响,特别关注了不同的植物间距和生长阶段。在降雨情况下,与老化植被相比,较年轻的植被能更有效地保持和恢复高达 25 kPa 的母质吸力。与光秃秃的斜坡相比,植被大大提高了安全系数,最高可达 0.3。植物生长和减少植物间距增加了根系对水力和机械稳定性的影响,这主要归因于根系内聚力的影响,而不是蒸腾率的影响。研究结果表明,由于植被引起的根系生长区内的内聚力,安全系数提高的机械贡献从三分之一提高到三分之二。
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引用次数: 0
期刊
Biogeotechnics
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