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Physical property of MICP-treated calcareous sand under seawater conditions by CPTU 用 CPTU 分析海水条件下经 MICP 处理的钙质砂的物理性质
Pub Date : 2024-10-22 DOI: 10.1016/j.bgtech.2024.100131
Kemeng Yu, Yuling Ran, Jie Shi, Menglan Duan, Zhongkun Ouyang
MICP (Microbially induced calcite precipitation), an environmentally friendly soil improvement technique, has great potential in ocean engineering due to its ability to promote the precipitation of calcium carbonate through microbial activity to enhance the engineering properties of geomaterials. In this study, piezocone penetration test (CPTU) is used to evaluate the effectiveness of MICP treatment in calcareous sand. The change of physical properties (relative density Dr and total unit weight γt) of MICP treated calcareous sand is investigated by conducting CPTU on the geomaterials prepared in a series of mini calibration chambers (25 cm × 50 cm). Results indicate that CPTU (tip stress, sleeve friction, and porewater pressure) measurements can be used to interpret the physical characteristics of calcareous sand treated with MICP under seawater conditions. Additionally, a relationship between CPTU measurements, physical parameters (relative density Dr and total unit weight γt) of MICP treated calcareous sand is proposed and calibrated. The findings of the research extend the implementation of in-situ testing techniques such as CPTU towards physical property evaluation of bio-treated geomaterials in ocean environment, and demonstrate the potential of scaling up MICP techniques for broader engineering application.
微生物诱导方解石沉淀(MICP)是一种环境友好型土壤改良技术,由于其能够通过微生物活动促进碳酸钙沉淀,从而提高土工材料的工程特性,因此在海洋工程中具有巨大潜力。在本研究中,采用压陷渗透试验(CPTU)来评估 MICP 处理钙质砂的效果。通过对在一系列微型校准室(25 厘米 × 50 厘米)中制备的土工材料进行 CPTU,研究了经 MICP 处理的钙质砂的物理性质(相对密度 Dr 和总单位重量 γt)的变化。结果表明,CPTU(尖端应力、套筒摩擦力和孔隙水压力)测量值可用于解释经 MICP 处理的钙质砂在海水条件下的物理特性。此外,还提出并校准了经 MICP 处理的钙质砂的 CPTU 测量值与物理参数(相对密度 Dr 和总单位重量 γt)之间的关系。研究结果将 CPTU 等原位测试技术的应用范围扩大到海洋环境中生物处理过的土工材料的物理性质评估,并证明了 MICP 技术在更广泛的工程应用中的扩展潜力。
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引用次数: 0
Miniaturized device to measure urease activity in the soil interstitial fluid using wenner method 利用温纳法测量土壤间隙液中尿素酶活性的微型装置
Pub Date : 2024-09-30 DOI: 10.1016/j.bgtech.2024.100120
Rafaela Cardoso , Thomas Drouinot , Susana Cardoso de Freitas
This paper presents a microdevice developed to measure the electrical conductivity of a liquid or a saturated porous medium using Wenner method. It is developed in the context of biocementation as soil improvement technique, which is used in Civil Engineering applications to produce calcium carbonate through bacterial or enzymatic activity, replacing the use of other binder materials such as cement or resins, and therefore reducing carbon footprint. The microdevice was used to measure urease activity in the soil interstitial fluid, to investigate if bacterial activity could be affected by the presence of the particles and tortuosity from pore geometry. Such analysis is important to understand biocementation mechanism inside the soil and helps to improve the design of such treatment solutions. The device is basically a squared reservoir printed in polypropylene using a 3D printing machine, incorporating stainless steel electrodes in its base. The electrical resistivity was computed adopting Wenner method, by connecting 4 PCB electrodes to a signal generator and an oscilloscope for measuring the voltage when a AC current of 1 mA was applied. Both square and sinusoidal waves with 5 kHz frequency were selected among other frequencies. The measurements were adjusted during the calibration of the microdevice, done using standard salt solutions with known electrical conductivity measured using an electrical conductivity probe. For the bacterial activity measurements, the bacterial and urea solutions were added to a uniform-graded size quarzitic sand (average diameter 0.3 mm) placed inside the microdevice and covering completely the electrodes. Bacterial activity was not affected by the presence of the sand, which confirms that this treatment is effective for this type of soils.
本文介绍了一种利用温纳法测量液体或饱和多孔介质电导率的微型装置。生物水泥化是一种土壤改良技术,应用于土木工程领域,通过细菌或酶的活性产生碳酸钙,取代水泥或树脂等其他粘结材料的使用,从而减少碳足迹。该微型装置用于测量土壤间隙流体中的脲酶活性,以研究细菌活性是否会受到颗粒存在和孔隙几何形状迂回的影响。此类分析对于了解土壤内部的生物降解机制非常重要,有助于改进此类处理方案的设计。该装置基本上是一个使用 3D 打印机用聚丙烯打印的方形蓄水池,其底部装有不锈钢电极。电阻率的计算采用温纳法,将 4 个 PCB 电极连接到信号发生器和示波器上,以测量施加 1 mA 交流电时的电压。在其他频率中,选择了频率为 5 kHz 的方波和正弦波。在校准微型设备时,使用标准盐溶液对测量结果进行调整,标准盐溶液的电导率是通过电导率探针测量得出的。在测量细菌活性时,将细菌溶液和尿素溶液加入放置在微装置内并完全覆盖电极的大小均匀的石英砂(平均直径 0.3 毫米)中。细菌活性并没有因为沙子的存在而受到影响,这证明这种处理方法对这类土壤是有效的。
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引用次数: 0
Development characteristics and quantitative analysis of cracks in root-soil complex during different growth periods under dry-wet cycles 干湿循环条件下不同生长期根-土复合体裂缝的发展特征和定量分析
Pub Date : 2024-07-25 DOI: 10.1016/j.bgtech.2024.100121
Zhengjun Mao , Xu Ma , Mimi Geng , Munan Wang , Guangsheng Gao , Yanshan Tian
Repeated wet swelling and dry shrinkage of soil leads to the gradual occurrence of cracks and the formation of a complex fracture network. In order to study the development characteristics and quantitative analysis of cracks in root-soil complex in different growth periods under dry-wet cycles, the alfalfa root-loess complex was investigated during different growth periods under different dry-wet cycles, and a dry-wet cycle experiment was conducted. The crack rate, relative area, average width, total length, and the cracks fractal dimension in the root-soil complex were extracted; the crack development characteristics of plain soil were analyzed under the PG-DWC (dry-wet cycle caused by plant water management during plant growth period), as well as the crack development characteristics of root-soil complex under PG-DWC and EC-DWC (the dry-wet cycles caused by extreme natural conditions such as continuous rain); the effects of plant roots and dry-wet cycles on soil cracks were discussed. The results showed that the average crack width, crack rate, relative crack area, and total crack length of the alfalfa root-loess complex were higher than those of the plain soil during PG-DWC. The result indicated that compared with plain soil during PG-DWC, the presence of plant roots in alfalfa root-soil complex in the same growth period promoted the cracks development to some extent. The alfalfa root-soil complex crack parameters during different growth periods were relatively stable during PG-DWC (0 dry-wet cycle). During EC-DWC (1, 3, and 5 dry-wet cycles), the alfalfa root-loess complex crack parameters increased with the number of dry-wet cycles during different growth periods. Unlike PG-DWC, the EC-DWC accelerated crack development, and the degree of crack development increased with the number of dry-wet cycles. The existence of plant roots promoted crack development and expansion in the root-soil complex to a certain extent, and the dry-wet cycle certainly promoted crack development and expansion in the root-soil complex. This result contradicts the improvement in the root-soil complex's macro-mechanical properties during plant growth, due to differences in the mechanical properties of roots and soil. The research results will provide reference for the root soil complex crack development law and the design of slope protection by vegetation.
土壤的反复湿胀干缩会导致裂缝的逐渐产生,并形成复杂的断裂网络。为了研究干湿循环条件下不同生长时期根-土复合体裂缝的发展特征和定量分析,研究了不同干湿循环条件下不同生长时期苜蓿根-土复合体的裂缝,并进行了干湿循环实验。提取了根-土复合体的裂缝率、相对面积、平均宽度、总长度和裂缝分形维数;分析了PG-DWC(植物生长期植物水分管理引起的干湿循环)条件下素土的裂缝发育特征,以及PG-DWC和EC-DWC(连续降雨等极端自然条件引起的干湿循环)条件下根-土复合体的裂缝发育特征;讨论了植物根系和干湿循环对土壤裂缝的影响。结果表明,在 PG-DWC 期间,苜蓿无根复合土壤的平均裂缝宽度、裂缝率、相对裂缝面积和总裂缝长度均高于普通土壤。结果表明,与 PG-DWC 期间的普通土壤相比,同一生长期内苜蓿根-土复合体中植物根系的存在在一定程度上促进了裂缝的发展。不同生长期的苜蓿根-土复合体裂缝参数在 PG-DWC 期间(0 干-湿循环)相对稳定。在 EC-DWC 期间(1、3 和 5 个干湿循环),不同生长期的苜蓿无根复合裂缝参数随干湿循环次数的增加而增加。与 PG-DWC 不同,EC-DWC 加快了裂纹的发展,而且裂纹发展程度随干湿循环次数的增加而增加。植物根系的存在在一定程度上促进了根-土复合体中裂纹的发展和扩展,而干湿循环无疑促进了根-土复合体中裂纹的发展和扩展。这一结果与植物生长过程中根-土复合体宏观力学性能的改善相矛盾,这是由于根和土的力学性能不同造成的。该研究成果将为根土复合体裂缝发展规律和植被护坡设计提供参考。
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引用次数: 0
Improved methods, properties, applications and prospects of microbial induced carbonate precipitation (MICP) treated soil: A review 微生物诱导碳酸盐沉淀 (MICP) 处理土壤的改进方法、特性、应用和前景:综述
Pub Date : 2024-07-18 DOI: 10.1016/j.bgtech.2024.100123
Xuanshuo Zhang , Hongyu Wang , Ya Wang , Jinghui Wang , Jing Cao , Gang Zhang
Soil improvement is one of the most important issues in geotechnical engineering practice. The wide application of traditional improvement techniques (cement/chemical materials) are limited due to damage ecological environment and intensify carbon emissions. However, the use of microbially induced calcium carbonate precipitation (MICP) to obtain bio-cement is a novel technique with the potential to induce soil stability, providing a low-carbon, environment-friendly, and sustainable integrated solution for some geotechnical engineering problems in the environment. This paper presents a comprehensive review of the latest progress in soil improvement based on the MICP strategy. It systematically summarizes and overviews the mineralization mechanism, influencing factors, improved methods, engineering characteristics, and current field application status of the MICP. Additionally, it also explores the limitations and correspondingly proposes prospective applications via the MICP approach for soil improvement. This review indicates that the utilization of different environmental calcium-based wastes in MICP and combination of materials and MICP are conducive to meeting engineering and market demand. Furthermore, we recommend and encourage global collaborative study and practice with a view to commercializing MICP technique in the future. The current review purports to provide insights for engineers and interdisciplinary researchers, and guidance for future engineering applications.
土壤改良是岩土工程实践中最重要的问题之一。传统改良技术(水泥/化学材料)的广泛应用受到限制,因为它们会破坏生态环境并加剧碳排放。然而,利用微生物诱导碳酸钙沉淀(MICP)获得生物水泥是一种新型技术,具有诱导土壤稳定性的潜力,可为环境中的一些岩土工程问题提供低碳、环保和可持续的综合解决方案。本文全面回顾了基于 MICP 策略的土壤改良的最新进展。它系统地总结和概述了 MICP 的矿化机理、影响因素、改良方法、工程特点和实地应用现状。此外,还探讨了 MICP 方法在土壤改良中的局限性,并相应地提出了应用前景。综述表明,在 MICP 中利用不同的环境钙基废物以及材料与 MICP 的结合有利于满足工程和市场需求。此外,我们建议并鼓励全球合作研究和实践,以期在未来实现 MICP 技术的商业化。本综述旨在为工程师和跨学科研究人员提供见解,并为未来的工程应用提供指导。
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引用次数: 0
Biogenic construction: The new era of civil engineering 生物建筑:土木工程的新时代
Pub Date : 2024-07-17 DOI: 10.1016/j.bgtech.2024.100130
Hanlong Liu
With the increasing demand for buildings and infrastructures and the mounting challenges associated with the current construction technologies such as high emission, high pollution, and high energy consumption, the civil engineering profession is at the crossroad for a transformation or upgrading before it can be put into tasks for these challenges. Inspired by the concept of harmonious coexistence between humans and nature, a new concept, biogenic construction, for civil engineering is proposed in this paper. The definition of biogenic construction is given. The framework and four components of biogenic construction are established. These include microbial construction, plant construction, animal construction, and bioinspired construction. Examples of each component are given. A new construction system for creating a more eco-friendly, healthier, and more sustainable environment for future civil engineering developments is also proposed.
随着建筑和基础设施需求的不断增长,以及当前建筑技术所面临的高排放、高污染和高能耗等日益严峻的挑战,土木工程专业正处于转型或升级的十字路口,以应对这些挑战。受人与自然和谐共生理念的启发,本文提出了土木工程的新理念--生源建筑。本文给出了生物建筑的定义。建立了生物建筑的框架和四个组成部分。其中包括微生物建筑、植物建筑、动物建筑和生物启发建筑。给出了每个组成部分的实例。此外,还提出了一种新的建筑系统,可为未来的土木工程发展创造更环保、更健康、更可持续的环境。
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引用次数: 0
Influence of grass plantation on the rainfall-induced instability of gentle loose fill slope 植草对降雨引起的松软填土缓坡失稳的影响
Pub Date : 2024-07-02 DOI: 10.1016/j.bgtech.2024.100101
Pei Tai , Fan Wu , Bohan Bai , Zhaofeng Li , Rui Chen , Lulu Zhang

The understanding of rainfall-induced landslides on gentle, loose-fill slopes is limited in comparison to steep slopes. Hence, two physical model tests were conducted on silty sand slopes under continuous rainfall: one on a bare slope and the other on a slope planted with ryegrass. The slope angle of 25° is much lower than the internal friction angle of slope material (34.3°), which makes the model test fall well into the category of gentle slope. For the initially unsaturated bare slope, a rainfall event with return period of 18 years could trigger a rapid and retrogressive global sliding, which differs from previous findings that gentle slopes would only experience shallow failure. A sudden increase in pore-water pressure was simultaneously observed, which might be generated by the wetting-induced collapse of unsaturated loose soil. On the other hand, the stability of the slope with grass plantation was significantly enhanced, and it was able to withstand rainfall event more severe than those with a return period of 100 years, with only minimal deformation. The results suggest that the gain in shear strength due to ryegrass roots surpasses the additional sliding force caused by the increased water retention capability. Additionally, it is found that the abrupt change in pore pressure was no longer indicative of slope failure in the case of the grass-reinforced slope.

与陡峭的斜坡相比,人们对降雨引起的松散填土缓坡滑坡的了解十分有限。因此,我们在连续降雨条件下对淤泥质沙质斜坡进行了两次物理模型试验:一次是在光秃秃的斜坡上,另一次是在种植了黑麦草的斜坡上。25° 的斜坡角远小于斜坡材料的内摩擦角(34.3°),因此模型试验完全属于缓坡范畴。对于最初未饱和的裸露斜坡来说,18 年一遇的降雨会引发快速的整体倒退滑动,这与以往认为缓坡只会发生浅层破坏的结论不同。同时观测到孔隙水压力突然增大,这可能是由于非饱和松散土壤受湿润诱发崩塌所致。另一方面,植草斜坡的稳定性明显增强,能够经受比重现期为 100 年的降雨事件更严重的降雨,而且变形很小。结果表明,黑麦草根系增加的抗剪强度超过了因蓄水能力增强而增加的滑动力。此外,研究还发现,在用草加固的斜坡上,孔隙压力的突然变化不再是斜坡崩塌的征兆。
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引用次数: 0
Effects of grassland vegetation roots on soil infiltration rate in Xiazangtan super large scale landslide distribution area in the upper reaches of the Yellow River, China 草地植被根系对中国黄河上游下藏滩特大型滑坡分布区土壤入渗率的影响
Pub Date : 2024-06-29 DOI: 10.1016/j.bgtech.2024.100104
Peihao Zhang , Guangyan Xing , Xiasong Hu , Changyi Liu , Xilai Li , Jimei Zhao , Jiangtao Fu , Haijing Lu , Huatan Li , Zhe Zhou , Lei Yue , Yabin Liu , Guorong Li , Haili Zhu

In order to study the infiltration characteristics of grassland soil in the super large scale landslides distribution area in the upper reaches of the Yellow River, this study selected the Xiazangtan super large scale distribution area in Jianzha County as the study area. Through experiments and numerical simulations, plant roots characteristics, soil physical properties and infiltration characteristics of naturally grazed grassland and enclosed grassland with different slope directions were compared and analyzed, and the influence of rainfall on seepage field and stability of the two grassland slopes were discussed. The results show that the highest soil moisture infiltration capacity (FIR) is found on the shady slope of the enclosed grassland (2.25), followed by the sunny slope of the enclosed grassland (1.23) and the shady slope of the naturally grazed grassland (−0.87). Correlation analysis show that soil water content, root dry weight density, total soil porosity, number of forks and root length are positively correlated with infiltration rate (P<0.05), whereas soil dry density is negatively correlated with infiltration rate (P<0.05). The results of stepwise regression analyses show that soil water content, total soil porosity, root length and number of forks are the main factors affecting soil infiltration capacity. And the ability of roots to increase soil infiltration by improving soil properties is higher than the effect of roots itself. After 60 min of simulated rainfall, the safety factors of the shady slopes of naturally grazed grassland and enclosed grassland are reduced by 29.56% and 19.63%, respectively, comparing to those before rainfall. Therefore, in this study, the roots play a crucial role in regulating soil infiltration and enhance slope stability by increasing soil water content, soil total porosity and shear strength while decreasing soil dry density. The results of this study provide theoretical evidence and practical guidance for the effective prevention and control of secondary geological disasters such as soil erosion and shallow landslide on the slope of river banks in the study area by using plant ecological measures.

为研究黄河上游超大规模滑坡分布区草地土壤的入渗特征,本研究选择尖扎县下藏滩超大规模滑坡分布区为研究区。通过试验和数值模拟,对比分析了不同坡向的天然放牧草地和围封草地的植物根系特征、土壤物理性质和入渗特征,探讨了降雨对两种草地边坡渗流场和稳定性的影响。结果表明,封闭草地阴坡的土壤水分入渗能力(FIR)最高(2.25),其次是封闭草地阳坡(1.23)和天然放牧草地阴坡(-0.87)。相关分析表明,土壤含水量、根干重密度、土壤总孔隙度、分叉数和根长与入渗率呈正相关(P<0.05),而土壤干密度与入渗率呈负相关(P<0.05)。逐步回归分析结果表明,土壤含水量、土壤总孔隙度、根系长度和分叉数是影响土壤入渗能力的主要因素。而根系通过改善土壤性质提高土壤入渗能力的作用高于根系本身的作用。模拟降雨 60 分钟后,与降雨前相比,自然放牧草地和封闭草地阴坡的安全系数分别降低了 29.56% 和 19.63%。因此,在本研究中,根系通过增加土壤含水量、土壤总孔隙度和抗剪强度,同时降低土壤干密度,在调节土壤渗透和增强边坡稳定性方面发挥了重要作用。本研究结果为利用植物生态措施有效防治研究区河岸坡面水土流失和浅层滑坡等次生地质灾害提供了理论依据和实践指导。
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引用次数: 0
Porosity and bedding controls on bio-induced carbonate precipitation and mechanical properties of shale and dolomitic rocks: EICP vs MICP 生物诱导碳酸盐沉淀及页岩和白云质岩石力学性能的孔隙度和层理控制:EICP 与 MICP
Pub Date : 2024-06-21 DOI: 10.1016/j.bgtech.2024.100102
Mary C. Ngoma , Oladoyin Kolawole

Biocementation is an emerging field within geotechnical engineering that focuses on harnessing microbiological activity to enhance the mechanical properties and behavior of rocks. It often relies on microbial-induced carbonate precipitation (MICP) or enzyme-induced carbonate precipitation (EICP) which utilizes biomineralization by promoting the generation of calcium carbonate (CaCO3) within the pores of geomaterials (rock and soil). However, there is still a lack of knowledge about the effect of porosity and bedding on biocementation in rocks from a mechanistic view. This experimental study investigated the impact of porosity and bedding orientations on the mechanical response of rocks due to biocementations, using two distinct biocementation strategies (MICP and EICP) and characteristically low porosity but interbedded rocks (shale) and more porous but non-bedded (dolostone) rocks. We first conducted biocementation treatments (MICP and EICP) of rock samples over a distinct period and temperature. Subsequently, the rock strength (uniaxial compressive strength, UCS) was measured. Finally, we analyzed the pre- and post-treatment changes in the rock samples to better understand the effect of MICP and EICP biocementations on the mechanical response of the rock samples. The results indicate that biocementations in dolostones can improve the rock mechanical integrity (EICP: +58% UCS; MICP: +25% UCS). In shales, biocementations can either slightly improve (EICP: +1% UCS) or weaken the rock mechanical integrity (MICP: −39% UCS). Further, results suggest that the major controlling mechanisms of biogeomechanical alterations due to MICP and EICP in rocks can be attributed to the inherent porosity, biocementation type, and bedding orientations, and in few cases the mechanisms can be swelling, osmotic suction, or pore pressurization. The findings in this study provide novel insights into the mechanical responses of rocks due to MICP and EICP biocementations.

生物加固是岩土工程中的一个新兴领域,其重点是利用微生物活动来增强岩石的机械性能和行为。它通常依靠微生物诱导碳酸盐沉淀(MICP)或酶诱导碳酸盐沉淀(EICP),通过促进岩土材料(岩石和土壤)孔隙中碳酸钙(CaCO3)的生成来利用生物矿化。然而,从机理角度来看,人们对孔隙度和垫层对岩石中生物降解的影响仍然缺乏了解。本实验研究利用两种不同的生物加固策略(MICP 和 EICP),以及孔隙率低但有层间的岩石(页岩)和孔隙率高但无层理的岩石(白云石),研究了孔隙率和层理方向对生物加固引起的岩石机械响应的影响。我们首先在不同时期和温度下对岩石样本进行生物水泥化处理(MICP 和 EICP)。随后,测量了岩石强度(单轴抗压强度,UCS)。最后,我们分析了岩石样本在处理前后的变化,以更好地了解 MICP 和 EICP 生物加固对岩石样本机械响应的影响。结果表明,在白云石中进行生物加固可以改善岩石的机械完整性(EICP:+58% UCS;MICP:+25% UCS)。在页岩中,生物加固既可以略微改善岩石的机械完整性(EICP:+1% UCS),也可以削弱岩石的机械完整性(MICP:-39% UCS)。此外,研究结果表明,岩石中的 MICP 和 EICP 导致生物地质力学改变的主要控制机制可归因于固有孔隙度、生物加固类型和层理方向,在少数情况下,其机制可能是膨胀、渗透吸力或孔隙增压。本研究的发现为了解岩石在 MICP 和 EICP 生物加固作用下的力学响应提供了新的视角。
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引用次数: 0
Bacterial attachment by crystal in MICP MICP 中的晶体附着细菌
Pub Date : 2024-06-04 DOI: 10.1016/j.bgtech.2024.100109

Microbially induced calcium carbonate precipitation (MICP) is recognized as a promising technique for soil improvement. The morphological evolution of calcium carbonate (CaCO3) crystals during the MICP process significantly impacts the engineering properties of biocemented soils. However, the morphological changes of CaCO3 precipitates upon bacterial adsorption onto crystal surfaces have not been sufficiently studied. This study employs real-time laser scanning confocal microscopy (LSCM) to simultaneously monitor the dynamics of CaCO3 growth and bacterial attachment during the MICP process, while fluorescence staining is used to differentiate between living and dead bacteria. The results indicate that during the initial stage of the MICP process, the predominant morphology of the CaCO3 crystals was elliptical, with a minor fraction exhibiting a rhombohedral morphology. Over time, additional elliptical crystals gradually formed around the existing elliptical ones. As the crystals grew, certain bacteria in the vicinity of the crystals became adsorbed onto their surfaces, irrespective of bacterial viability. However, bacterial adsorption did not alter the morphology of the crystals. The study provides microscale insights into the mechanisms of bacterial attachment to CaCO3 crystals during biomineralization.

微生物诱导碳酸钙沉淀(MICP)被认为是一种前景广阔的土壤改良技术。微生物诱导碳酸钙沉淀(MICP)过程中碳酸钙(CaCO3)晶体的形态演变会对生物加固土壤的工程特性产生重大影响。然而,对于细菌吸附到晶体表面时 CaCO3 沉淀的形态变化还没有进行充分的研究。本研究采用实时激光扫描共聚焦显微镜 (LSCM) 同时监测 MICP 过程中 CaCO3 的生长和细菌附着的动态,并利用荧光染色来区分活菌和死菌。结果表明,在 MICP 过程的初始阶段,CaCO3 晶体的主要形态是椭圆形,一小部分呈现斜方体形态。随着时间的推移,在原有的椭圆形晶体周围逐渐形成了更多的椭圆形晶体。随着晶体的生长,晶体附近的某些细菌被吸附到晶体表面,而与细菌的活力无关。然而,细菌的吸附并没有改变晶体的形态。这项研究从微观角度揭示了细菌在生物矿化过程中吸附到 CaCO3 晶体的机制。
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引用次数: 0
Development and optimization of biomimetic-chemically induced carbonate precipitation: A review of recent research 仿生化学诱导碳酸盐沉淀的开发与优化:最新研究综述
Pub Date : 2024-06-03 DOI: 10.1016/j.bgtech.2024.100110
Yu Diao , Jitao Bai , Gang Zheng , Qingsong Hu , Pengjin Li , Xuanqi Liu , Wendi Hu , Jianyou Huang
With further investigation on biomineralization, biomimetic mineralization has been proposed in imitation of microorganism behavior, in which the mechanism of biomineralization is utilized for the control of the crystal growth to synthesize inorganic materials with special structures and superior physical-chemical properties. This review summarizes the recent advances in biomimetic-chemically induced carbonate precipitation (BCICP). BCICP is a biomimetic mineralization process induced by calcium carbonate crystal modifiers, which directly regulates the metathesis reaction of calcium salts with carbonates in soils to improve the soil properties. Several crystal modifiers for BCICP, including the aspartic acid (organic), the boric acid (inorganic), and the polyacrylic acid (polymer), are reviewed, and the biomimetic mineralization mechanism is introduced. In addition, current findings about BCICP in cementitious materials, soil reinforcement, dust suppression, as well as other fields are discussed, aiming to give deeper insights into the further development and application of BCICP.
随着对生物矿化的进一步研究,人们提出了模仿微生物行为的生物仿生矿化,即利用生物矿化的机制控制晶体生长,从而合成具有特殊结构和优异物理化学性能的无机材料。本综述总结了生物仿生-化学诱导碳酸盐沉淀(BCICP)的最新进展。BCICP 是碳酸钙晶体改性剂诱导的生物模拟矿化过程,它直接调节土壤中钙盐与碳酸盐的偏析反应,从而改善土壤性质。本文综述了 BCICP 的几种晶体改性剂,包括天冬氨酸(有机)、硼酸(无机)和聚丙烯酸(聚合物),并介绍了其生物仿生矿化机制。此外,还讨论了目前有关 BCICP 在胶凝材料、土壤加固、抑尘以及其他领域的研究成果,旨在为 BCICP 的进一步开发和应用提供更深入的见解。
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Biogeotechnics
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