Bioimaging最新文献

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Signal sinc-interpolation: a fast computer algorithm 信号自插补:一种快速计算机算法

Bioimaging

Pub Date : 1996-12-01 DOI: 10.1002/1361-6374(199612)4:4<225::AID-BIO1>3.0.CO;2-G

L. Yaroslavsky

An efficient algorithm for discrete signal sinc-interpolation that is suitable for use in image and signal processing is described. Being mathematically equivalent to the commonly used zero padding interpolation method, the algorithm surpasses it in terms of flexibility, computational complexity and usage of computer memory.

介绍了一种适用于图像和信号处理的离散信号自插补算法。该算法在数学上与常用的零填充插值方法等效，在灵活性、计算复杂度和计算机内存使用方面都超过了它。

引用次数: 31

Systematic detection of subtle spatio–temporal patterns in time‐lapse imaging: I. Mitosis 延时成像中细微时空模式的系统检测:1 .有丝分裂

Bioimaging

Pub Date : 1996-12-01 DOI: 10.1002/1361-6374(199612)4:4<232::AID-BIO2>3.0.CO;2-L

R. Valdés-Pérez

A recent article on syncytial nuclear divisions in early embryos of Drosophila melanogaster presented evidence that the complex spatio–temporal data obtained from time-lapse images were patterned, contrary to previous suggestions based on preliminary observations. However, the reasoning that led to the hypothesis of patterning was informal and unsystematic. Here, we propose a systematic and computerized method for detecting subtle spatio–temporal mitotic patterns, under a general formulation of mitosis as three-dimensional asynchronous processes, of which the earlier data are a special case. The approach involves a rather elaborate application of the concept of permutation test from nonparametric statistics. Far from being limited to mitotic processes, the approach holds general promise for other classes of mass, time-lapse imaging phenomena in cell and developmental biology, such as migration and cell death.

最近一篇关于黑腹果蝇早期胚胎合胞核分裂的文章提出了证据，证明从延时图像中获得的复杂时空数据是有图案的，这与先前基于初步观察的建议相反。然而，导致模式假设的推理是非正式和不系统的。在这里，我们提出了一种系统的和计算机化的方法来检测细微的时空有丝分裂模式，在有丝分裂的一般公式下，作为三维异步过程，其中早期的数据是一个特殊的情况。该方法涉及非参数统计的置换检验概念的相当详细的应用。这种方法不仅局限于有丝分裂过程，而且对细胞和发育生物学中其他种类的质量、延时成像现象(如迁移和细胞死亡)也有普遍的前景。

引用次数: 5

Bio-speckle flowmetry for retinal blood flow diagnostics 生物斑点血流法用于视网膜血流诊断

Bioimaging

Pub Date : 1996-12-01 DOI: 10.1002/1361-6374(199612)4:4<254::AID-BIO4>3.0.CO;2-7

Y. Aizu, T. Asakura, K. Ogino, T. Sugita, Yasuyuki Suzuki, K. Masuda

Bio-speckle flowmetry for measuring the retinal blood flow velocity is described. The measuring principle is briefly discussed in comparison with the laser Doppler method. The basic properties of the photon correlation measurements including reproducibility were experimentally investigated with a rotating ground glass disk and for the normal human retina. The error was estimated to be less than 20% for in vivo measurements. By using a glass capillary model, the reciprocal of correlation time was calibrated to the mean flow velocity with a consideration of the effects of the vessel diameter and the background reflectance. The blood flow volume rate in the human retina was estimated by using the calibrated velocity and the vessel diameter. The results compared well with those reported in the literature, and show the usefulness of this flowmetry for clinical diagnostic purposes.

描述了用于测量视网膜血流速度的生物散斑血流法。简要讨论了测量原理，并与激光多普勒法进行了比较。用旋转磨砂玻璃盘和正常人视网膜实验研究了光子相关测量的基本性质，包括再现性。体内测量的误差估计小于20%。利用玻璃毛细管模型，在考虑血管直径和背景反射率影响的情况下，将相关时间倒数标定为平均流速。利用标定后的流速和血管直径来估计人视网膜的血流体积率。结果与文献报道的结果相比较，显示了该血流法在临床诊断中的实用性。

引用次数: 19

Laser speckle spectroscopy—a new method for using small swimming organisms as biomonitors 激光散斑光谱法——一种利用小型游泳生物作为生物监测器的新方法

Bioimaging

Pub Date : 1996-12-01 DOI: 10.1002/1361-6374(199612)4:4<243::AID-BIO3>3.0.CO;2-E

J A Cole, M H Tinker

A novel method has been devised for the study of swimming organisms by using speckle patterns produced by their scattering of coherent laser light. The speckle patterns show fluctuations in space and time which may be correlated with the activity of the organisms. The fluctuations give an immediate indication of mobility and a more detailed analysis of the frequency spectrum of the speckle fluctuations shows characteristic resonance-like features which are specific to the organism. The speckle patterns produced by several protozoans, including Paramecium bursaria, Entosiphon sulcatum, and by the alga Chlamydomonas reinhardii and the rotifer Brachionus calyciflorus have been studied. Laser speckle spectroscopy (LSS) allows a rapid non-invasive monitoring of the activity of the organisms and could find application in ecotoxicity studies and environmental biomonitoring. The results presented here are the first reports of LSS and its use in this way and demonstrate its viability and potential for further development.

利用相干激光散射产生的散斑图案，设计了一种研究游动生物的新方法。散斑图案显示了空间和时间上的波动，这可能与生物体的活动有关。波动提供了流动性的即时指示，并且对散斑波动的频谱的更详细分析显示了生物体特有的特征性共振样特征。研究了几种原生动物，包括草履虫、沟虫、莱茵衣藻和杯状臂尾轮虫产生的斑点图案。激光散斑光谱（LSS）可以对生物体的活动进行快速的非侵入性监测，并可应用于生态毒性研究和环境生物监测。本文给出的结果是LSS及其以这种方式使用的首次报告，并证明了其可行性和进一步开发的潜力。

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引用次数: 4

Laser speckle spectroscopy—a new method for using small swimming organisms as biomonitors 激光散斑光谱——一种利用小型游动生物作为生物监测仪的新方法

Bioimaging

Pub Date : 1996-12-01 DOI: 10.1002/1361-6374(199612)4:4<243::AID-BIO3>3.0.CO;2-E

J. Cole, M. Tinker

A novel method has been devised for the study of swimming organisms by using speckle patterns produced by their scattering of coherent laser light. The speckle patterns show fluctuations in space and time which may be correlated with the activity of the organisms. The fluctuations give an immediate indication of mobility and a more detailed analysis of the frequency spectrum of the speckle fluctuations shows characteristic resonance-like features which are specific to the organism. The speckle patterns produced by several protozoans, including Paramecium bursaria, Entosiphon sulcatum, and by the alga Chlamydomonas reinhardii and the rotifer Brachionus calyciflorus have been studied. Laser speckle spectroscopy (LSS) allows a rapid non-invasive monitoring of the activity of the organisms and could find application in ecotoxicity studies and environmental biomonitoring. The results presented here are the first reports of LSS and its use in this way and demonstrate its viability and potential for further development.

一种新的方法已经被设计出来用于研究游泳生物，利用它们的相干激光散射产生的斑点图案。斑点图案显示了时空的波动，这可能与生物体的活动有关。这些波动可以立即显示出迁移率，对散斑波动的频谱进行更详细的分析，可以显示出生物体特有的特征共振。本文研究了几种原生动物，包括滑囊草履虫、沟内虹吸、reinhardii衣藻和萼花臂轮虫所产生的斑点图案。激光散斑光谱(LSS)可以对生物活性进行快速无创监测，在生态毒性研究和环境生物监测中具有广泛的应用前景。本文介绍的结果是LSS及其以这种方式使用的首次报告，并展示了其可行性和进一步发展的潜力。

引用次数: 4

Systematic detection of subtle spatio–temporal patterns in time-lapse imaging: I. Mitosis 延时成像中细微时空模式的系统检测：I.有丝分裂

Bioimaging

Pub Date : 1996-12-01 DOI: 10.1002/1361-6374(199612)4:4<232::AID-BIO2>3.0.CO;2-L

Raúl E Valdés-Pérez

A recent article on syncytial nuclear divisions in early embryos of Drosophila melanogaster presented evidence that the complex spatio–temporal data obtained from time-lapse images were patterned, contrary to previous suggestions based on preliminary observations. However, the reasoning that led to the hypothesis of patterning was informal and unsystematic. Here, we propose a systematic and computerized method for detecting subtle spatio–temporal mitotic patterns, under a general formulation of mitosis as three-dimensional asynchronous processes, of which the earlier data are a special case. The approach involves a rather elaborate application of the concept of permutation test from nonparametric statistics. Far from being limited to mitotic processes, the approach holds general promise for other classes of mass, time-lapse imaging phenomena in cell and developmental biology, such as migration and cell death.

最近一篇关于黑腹果蝇早期胚胎合胞体细胞核分裂的文章提供了证据，证明从延时图像中获得的复杂时空数据是模式化的，这与之前基于初步观察的建议相反。然而，导致模式化假设的推理是非正式的和不系统的。在这里，我们提出了一种系统和计算机化的方法来检测细微的时空有丝分裂模式，在有丝分裂为三维异步过程的一般公式下，早期的数据是一种特殊情况。该方法涉及非参数统计学中排列检验概念的相当详细的应用。该方法不仅限于有丝分裂过程，而且对细胞和发育生物学中的其他类型的大规模延时成像现象，如迁移和细胞死亡，都有普遍的前景。

引用次数: 5

Signal sinc-interpolation: A fast computer algorithm 信号sinc插值：一种快速的计算机算法

Bioimaging

Pub Date : 1996-12-01 DOI: 10.1002/1361-6374(199612)4:4<225::AID-BIO1>3.0.CO;2-G

L P Yaroslavsky

介绍了一种适用于图像和信号处理的离散信号sinc插值的有效算法。该算法在数学上等同于常用的零填充插值方法，在灵活性、计算复杂性和计算机内存使用方面都优于它。

引用次数: 31

Avalanche photodiode detection with object scanning and image restoration provides 2–4 fold resolution increase in two‐photon fluorescence microscopy 雪崩光电二极管检测与对象扫描和图像恢复提供2-4倍的分辨率增加在双光子荧光显微镜

Bioimaging

Pub Date : 1996-09-01 DOI: 10.1002/1361-6374(199609)4:3<187::AID-BIO9>3.0.CO;2-3

H. Kano, H. M. Voort, M. Schrader, Geert M. P. van Kempen, S. Hell

High-quantum-efficiency photodetection, millisecond pixel dwell time stage scanning and image restoration by maximum-likelihood estimation are synergetically combined and shown to improve the resolution of two-photon excitation microscopy 2–4 fold in all directions. Measurements of the two-photon excitation point-spread function (PSF) of a 1.4 aperture oil immersion lens are carried out by imaging fluorescence beads with a diameter of one seventh of the excitation wavelength (830 nm) and subsequent deconvolution with the bead object function. The proposed method of resolution increase is applied to beads as well as to rhodamine labelled actin fibres in mouse fibroblast cells. As the resolution improvement is not based on the non-linear effect of two-photon excitation, the results imply a comparable resolution increase in single-photon excitation confocal microscopy. In the fibroblasts, we established a three-fold improvement in axial resolution, namely from 840 nm before, to 280 nm after restoration (full-width at half-maximum). Actin fibres with axial distances of 850 nm, otherwise difficult to discern, are fully separated. In the lateral direction, images of fluorescence beads of about 110 nm diameter are restored to the real dimensions of the beads with an accuracy of better than one pixel (41 nm).

高量子效率的光探测、毫秒像素停留时间阶段扫描和最大似然估计图像恢复协同结合，在各个方向上都将双光子激发显微镜的分辨率提高了2-4倍。采用直径为激发波长(830nm)的七分之一的荧光珠成像，并与珠目标函数反褶积，测量了1.4孔径油浸透镜的双光子激发点扩展函数(PSF)。提出的提高分辨率的方法适用于珠以及罗丹明标记的肌动蛋白纤维在小鼠成纤维细胞。由于分辨率的提高不是基于双光子激发的非线性效应，结果表明单光子激发共聚焦显微镜的分辨率提高相当。在成纤维细胞中，我们发现轴向分辨率提高了三倍，即从修复前的840 nm提高到修复后的280 nm(全宽半最大值)。轴向距离为850纳米的肌动蛋白纤维完全分离，否则很难识别。在横向上，将直径约为110 nm的荧光珠的图像恢复到珠的真实尺寸，精度优于1像素(41 nm)。

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引用次数: 35

Effect of the detector size and the fluorescence wavelength on the resolution of three‐ and two‐photon confocal microscopy 探测器尺寸和荧光波长对三光子和二光子共聚焦显微镜分辨率的影响

Bioimaging

Pub Date : 1996-09-01 DOI: 10.1002/1361-6374(199609)4:3<129::AID-BIO3>3.0.CO;2-L

M. Gu, X. Gan

引用次数: 13

Two‐photon laser scanning fluorescence microscopy‐from a fluorophore and specimen perspective 双光子激光扫描荧光显微镜-从荧光团和标本的角度

Bioimaging

Pub Date : 1996-09-01 DOI: 10.1002/1361-6374(199609)4:3<168::AID-BIO7>3.0.CO;2-8

J. Bhawalkar, A. Shih, Sinno Jialin Pan, W. Liou, J. Swiatkiewicz, B. Reinhardt, P. N. Prasad, P. Cheng

引用次数: 24

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