Ankan Kheto, Uttam Adhikary, Subhamoy Dhua, Ayan Sarkar, Yogesh Kumar, Rahul Vashishth, Bibek Bahadur Shrestha, D. C. Saxena
Whey protein (WP) isolates are widely used in food products because they improve protein content, functional properties, and health benefits. It has been observed that WP can form conjugates with essential micronutrients, improving their dietary delivery and biological availability. WP and its derived bioactive peptides have potential health benefits in preventing chronic diseases. However, the off-flavor and bitter peptides of WP are limiting factors that can be used in the food and beverage industries. Emerging innovative processing technologies, such as high hydrostatic pressure, ultrasonication, and extrusion, improve WP gelling ability, gel strength, and storage stability, providing high-quality and healthy functional products. The integration of technological advancements into WP-loaded fortified food products (FFPs) has increased the uptake rate of bioactive compounds, resulting in increased bioavailability and bioaccessibility in the human body. Furthermore, WP-based FFPs treated with advanced thermal and nonthermal processing techniques possess better rheological properties, expansion ratios, and textural properties. Therefore, WP would be a promising and sustainable ingredient for developing stable functional foods. This review explores recent advancements in the processing technologies of WP, emphasizing their role in enhancing its bioavailability and functional properties for different food applications.
{"title":"A review on advancements in emerging processing of whey protein: Enhancing functional and nutritional properties for functional food applications","authors":"Ankan Kheto, Uttam Adhikary, Subhamoy Dhua, Ayan Sarkar, Yogesh Kumar, Rahul Vashishth, Bibek Bahadur Shrestha, D. C. Saxena","doi":"10.1002/fsh3.12067","DOIUrl":"https://doi.org/10.1002/fsh3.12067","url":null,"abstract":"<p>Whey protein (WP) isolates are widely used in food products because they improve protein content, functional properties, and health benefits. It has been observed that WP can form conjugates with essential micronutrients, improving their dietary delivery and biological availability. WP and its derived bioactive peptides have potential health benefits in preventing chronic diseases. However, the off-flavor and bitter peptides of WP are limiting factors that can be used in the food and beverage industries. Emerging innovative processing technologies, such as high hydrostatic pressure, ultrasonication, and extrusion, improve WP gelling ability, gel strength, and storage stability, providing high-quality and healthy functional products. The integration of technological advancements into WP-loaded fortified food products (FFPs) has increased the uptake rate of bioactive compounds, resulting in increased bioavailability and bioaccessibility in the human body. Furthermore, WP-based FFPs treated with advanced thermal and nonthermal processing techniques possess better rheological properties, expansion ratios, and textural properties. Therefore, WP would be a promising and sustainable ingredient for developing stable functional foods. This review explores recent advancements in the processing technologies of WP, emphasizing their role in enhancing its bioavailability and functional properties for different food applications.</p>","PeriodicalId":100546,"journal":{"name":"Food Safety and Health","volume":"3 1","pages":"23-45"},"PeriodicalIF":0.0,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fsh3.12067","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143116329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Alessandro Di Minno, Maria Vittoria Morone, Hammad Ullah, Eduardo Sommella, Daniele Giuseppe Buccato, Lorenza Francesca De Lellis, Pietro Campiglia, Anna De Filippis, Massimiliano Galdiero, Maria Daglia
Estimating the hydroxyanthracene derivative (HAD) concentrations and assessing the genotoxicity of several marketed Aloe vera (L.) Burm. f. gel beverages are the goals of this study. The results showed that five commercial samples contained aloin A at concentrations ranging from 6.05 to 337.98 ng/mL and aloin B at concentrations ranging from 8.84 to 346.89 ng/mL. Four commercial samples contained aloin A and B in concentration higher than 1 ppm, while one sample contained less than 1 ppm. Aloe-emodin was detected in three samples with concentrations ranging from 80.30 to 109.40 ng/mL. Different strains of bacteria were used to perform the Ames test on the samples with the lowest and highest HAD concentrations, and no signs of mutagenicity were found in experiments with samples that increased in concentration (0.0016–5 μL/mL). In accordance with published data and in contrast to findings acquired using products containing A. vera latex, which is rich in HADs and whose genotoxicity is extensively proven, even the sample with the highest concentrations of aloin A and B does not show any in vitro genotoxicity, suggesting the importance to standardize the operating procedures to obtain A. vera gel to minimize the content of HADs in the commercial formulations.
本研究的目的是估算几种市场上销售的芦荟(L.)Burm.f.凝胶饮料的羟基蒽衍生物(HAD)浓度并评估其遗传毒性。结果表明,五个商业样品中芦荟素 A 的浓度范围为 6.05 至 337.98 纳克/毫升,芦荟素 B 的浓度范围为 8.84 至 346.89 纳克/毫升。四个商业样品中的芦荟甙 A 和 B 含量高于百万分之 1,而一个样品中的含量低于百万分之 1。在三个样品中检测到了芦荟大黄素,浓度在 80.30 至 109.40 纳克/毫升之间。使用不同的细菌菌株对 HAD 浓度最低和最高的样品进行 Ames 试验,在浓度增加(0.0016-5 μL/mL)的样品实验中没有发现诱变迹象。根据已公布的数据,即使是阿洛因 A 和 B 含量最高的样本也没有显示出任何体外遗传毒性,这与使用含有阿洛因乳胶(阿洛因乳胶富含 HADs,其遗传毒性已得到广泛证实)的产品所获得的结果形成鲜明对比,这表明必须规范获取阿洛因凝胶的操作程序,以尽量减少商业配方中的 HADs 含量。
{"title":"Liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based quantification of hydroxyanthracene derivatives in Aloe vera (L.) Burm. f. gel commercial beverages and preliminary safety evaluation through in vitro genotoxicity studies","authors":"Alessandro Di Minno, Maria Vittoria Morone, Hammad Ullah, Eduardo Sommella, Daniele Giuseppe Buccato, Lorenza Francesca De Lellis, Pietro Campiglia, Anna De Filippis, Massimiliano Galdiero, Maria Daglia","doi":"10.1002/fsh3.12064","DOIUrl":"https://doi.org/10.1002/fsh3.12064","url":null,"abstract":"<p>Estimating the hydroxyanthracene derivative (HAD) concentrations and assessing the genotoxicity of several marketed <i>Aloe vera</i> (L.) Burm. f. gel beverages are the goals of this study. The results showed that five commercial samples contained aloin A at concentrations ranging from 6.05 to 337.98 ng/mL and aloin B at concentrations ranging from 8.84 to 346.89 ng/mL. Four commercial samples contained aloin A and B in concentration higher than 1 ppm, while one sample contained less than 1 ppm. Aloe-emodin was detected in three samples with concentrations ranging from 80.30 to 109.40 ng/mL. Different strains of bacteria were used to perform the Ames test on the samples with the lowest and highest HAD concentrations, and no signs of mutagenicity were found in experiments with samples that increased in concentration (0.0016–5 μL/mL). In accordance with published data and in contrast to findings acquired using products containing <i>A. vera</i> latex, which is rich in HADs and whose genotoxicity is extensively proven, even the sample with the highest concentrations of aloin A and B does not show any in vitro genotoxicity, suggesting the importance to standardize the operating procedures to obtain <i>A. vera</i> gel to minimize the content of HADs in the commercial formulations.</p>","PeriodicalId":100546,"journal":{"name":"Food Safety and Health","volume":"2 4","pages":"489-496"},"PeriodicalIF":0.0,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fsh3.12064","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142430026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Heavy metals represent significant environmental pollutants in today's world. The primary sources of human exposure to these heavy metals are through food and water, which can lead to various life-threatening diseases. Therefore, it is imperative to focus on the removal of these contaminants to safeguard human health. The study describes the optimization of biosorption conditions of chromium [Cr(III)] and zinc [Zn(II)] through biofilm and exopolysaacharides (EPS) of Lactobacillus plantarum. The strain was tested for minimum inhibitory concentration and assessed at 100–350 ppm for Cr (III) and Zn (II) in terms of log cfu/mL. Metal adsorption capacity of biofilm was slightly higher as compared to EPS. Biosorption of Cr (III) at pH 2 showed 90% biosorption through biofilms and 88% through EPS. Zn (II) showed maximum binding of about 86% and 78% by biofilm and EPS, respectively, at pH 8 at 37°C for 24 h. The binding of metal is mostly dependent upon the functional group present, the surface morphology of biofilm, and the EPS of strain.
{"title":"Simultaneous biosorption of chromium and zinc from aqueous solution by Lactobacillus plantarum exopolysaccharides and biofilm characterized by structural and functional analysis","authors":"Anchal Singh, Mazia Ahmed, Unaiza Iqbal, Urvashi Srivastava, Pinki Saini","doi":"10.1002/fsh3.12065","DOIUrl":"https://doi.org/10.1002/fsh3.12065","url":null,"abstract":"<p>Heavy metals represent significant environmental pollutants in today's world. The primary sources of human exposure to these heavy metals are through food and water, which can lead to various life-threatening diseases. Therefore, it is imperative to focus on the removal of these contaminants to safeguard human health. The study describes the optimization of biosorption conditions of chromium [Cr(III)] and zinc [Zn(II)] through biofilm and exopolysaacharides (EPS) of <i>Lactobacillus plantarum</i>. The strain was tested for minimum inhibitory concentration and assessed at 100–350 ppm for Cr (III) and Zn (II) in terms of log cfu/mL. Metal adsorption capacity of biofilm was slightly higher as compared to EPS. Biosorption of Cr (III) at pH 2 showed 90% biosorption through biofilms and 88% through EPS. Zn (II) showed maximum binding of about 86% and 78% by biofilm and EPS, respectively, at pH 8 at 37°C for 24 h. The binding of metal is mostly dependent upon the functional group present, the surface morphology of biofilm, and the EPS of strain.</p>","PeriodicalId":100546,"journal":{"name":"Food Safety and Health","volume":"2 4","pages":"497-508"},"PeriodicalIF":0.0,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fsh3.12065","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142429721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Harshad Dhaigude, Charanjiv Singh Saini, Mohd Aaqib Sheikh
Edible composite films from sunflower protein isolate emulsion (SPIE) and potato starch were prepared at different ratios by the casting method. The effect of starch addition on the barrier, mechanical, thermal, and morphological properties were investigated. The maximum tensile strength and minimum water vapor permeability was observed for composite film developed at 50:50 (SPIE:PS) ratio. Transparency of sunflower protein isolate emulsion film was observed to be lower as compared to all other composite films. Total color difference values increased with increase in the concentration of starch. X-ray diffraction of SPI emulsion film and composite films indicated amorphous structure of films. SPI emulsion film exhibited high degree of porosity. With the addition of starch, porosity of films decreased, and compactness increased. Differential scanning calorimetry curves of all films indicated melting temperature in the range of 87.22–157.21°C and enthalpy in the range of 39.86–110.94 J/g. Overall, composite films were compact, nonporous and smooth as compared to film prepared from native SPI emulsion.
{"title":"Development of sunflower protein isolate emulsion-based edible composite films with the addition of potato starch: Barrier, mechanical, thermal, and morphological properties","authors":"Harshad Dhaigude, Charanjiv Singh Saini, Mohd Aaqib Sheikh","doi":"10.1002/fsh3.12062","DOIUrl":"https://doi.org/10.1002/fsh3.12062","url":null,"abstract":"<p>Edible composite films from sunflower protein isolate emulsion (SPIE) and potato starch were prepared at different ratios by the casting method. The effect of starch addition on the barrier, mechanical, thermal, and morphological properties were investigated. The maximum tensile strength and minimum water vapor permeability was observed for composite film developed at 50:50 (SPIE:PS) ratio. Transparency of sunflower protein isolate emulsion film was observed to be lower as compared to all other composite films. Total color difference values increased with increase in the concentration of starch. X-ray diffraction of SPI emulsion film and composite films indicated amorphous structure of films. SPI emulsion film exhibited high degree of porosity. With the addition of starch, porosity of films decreased, and compactness increased. Differential scanning calorimetry curves of all films indicated melting temperature in the range of 87.22–157.21°C and enthalpy in the range of 39.86–110.94 J/g. Overall, composite films were compact, nonporous and smooth as compared to film prepared from native SPI emulsion.</p>","PeriodicalId":100546,"journal":{"name":"Food Safety and Health","volume":"2 4","pages":"465-479"},"PeriodicalIF":0.0,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fsh3.12062","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142430264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The chemical constituents of Myrica esculenta (a plant distributed across Nepal, India, China, and Pakistan) are known for their potent antioxidant properties and reported pharmacological effects. The study used methanol extract to assess antioxidant activity (DPPH assay), anthelmintic effects (standard; albendazole and experimental organism; Eisenia fetida), anti-inflammatory properties (human red blood cell membrane stabilization), and cytotoxicity (brine shrimp lethality bioassay). The study revealed 39.06% yield with methanol and 2.01% with hexane. Phenolic and flavonoid content were measured at 96.43 mg GAE/g and 14.54 mg QE/g, respectively. Methanolic extract exhibited IC50 of 60.89 μg/mL and LC5o of 160.5298 μg/mL. It also demonstrated anti-inflammatory activity, notably achieving a 37.8% membrane stabilizing action at 500 μg/mL. Anthelmintic potential was observed at different concentrations (50 mg/mL, 75 mg/mL, and 100 mg/mL), leading to paralysis and death of worms with corresponding times reported. Brine Shrimp Lethality Bioassay revealed concentration-dependent effects. Methanolic extract of M. esculenta displayed robust antioxidant and anthelmintic activities. The extract was identified as bioactive, holding promise as a source for plant-derived antitumor compounds. However, further investigation is essential to validate these findings.
{"title":"Bioactivity evaluations from stem bark extract fractions of Myrica esculenta Buch. -Ham.ex D. Don","authors":"Asmita Khanal, Bechan Raut, Dharma Prasad Khanal, Niranjan Koirala","doi":"10.1002/fsh3.12063","DOIUrl":"https://doi.org/10.1002/fsh3.12063","url":null,"abstract":"<p>The chemical constituents of <i>Myrica esculenta</i> (a plant distributed across Nepal, India, China, and Pakistan) are known for their potent antioxidant properties and reported pharmacological effects. The study used methanol extract to assess antioxidant activity (DPPH assay), anthelmintic effects (standard; albendazole and experimental organism; <i>Eisenia fetida</i>), anti-inflammatory properties (human red blood cell membrane stabilization), and cytotoxicity (brine shrimp lethality bioassay). The study revealed 39.06% yield with methanol and 2.01% with hexane. Phenolic and flavonoid content were measured at 96.43 mg GAE/g and 14.54 mg QE/g, respectively. Methanolic extract exhibited IC<sub>50</sub> of 60.89 μg/mL and LC<sub>5o</sub> of 160.5298 μg/mL. It also demonstrated anti-inflammatory activity, notably achieving a 37.8% membrane stabilizing action at 500 μg/mL. Anthelmintic potential was observed at different concentrations (50 mg/mL, 75 mg/mL, and 100 mg/mL), leading to paralysis and death of worms with corresponding times reported. Brine Shrimp Lethality Bioassay revealed concentration-dependent effects. Methanolic extract of <i>M</i>. <i>esculenta</i> displayed robust antioxidant and anthelmintic activities. The extract was identified as bioactive, holding promise as a source for plant-derived antitumor compounds. However, further investigation is essential to validate these findings.</p>","PeriodicalId":100546,"journal":{"name":"Food Safety and Health","volume":"2 4","pages":"480-488"},"PeriodicalIF":0.0,"publicationDate":"2024-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fsh3.12063","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142430179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Schematic diagram describing the reverse osmosis system applied to recover phenols from olive mill wastewater that can be used to fortify orange juice obtaining a functional beverage added with a natural antioxidant concentrate.