ITBM-RBM最新文献

Objective

Develop the methodology of data acquisition and processing for functional hepatobiliary scintigraphic imaging in normal mice.

Material and methods

The scintigraphic study was done on 11 female adult CD1 mice under gazeous anaesthesia using a small animal dedicated gamma-camera. A dynamic serie of 40 frames with 1 minute per frame was acquired after injection of Tc-99m mebrofenin. Two individuals were used for tomodensitometric morphological acquisitions using an X-ray micro-CT and the injection of a contrast agent and contributed to the choice of the scintigraphic regions of interest. Four analytical models: corrected and deconvolved hepatogram (A), bi-compartmental model (B), fast kinetics compartmental model (C) and factorial analysis (D) were applied to the scintigraphic data starting from areas of interest centered to the cardiac cavities and the liver.

Results

The mebrofenin kinetics is characterized by a time of the maximum t_max ranging between 4.5 and 4.6 min, a time of the center of gravity t_cdg from 6.2 to 7.0 min, a half-life of exponential decay t_1/2decr from 5.4 to 6.8 min, a fraction of hepatic extraction of 100%. Uptake and excretion half-lives are 2.3 and 5.8 min for the bicompratmental model and are 3.2 and 3.3 min for the fast kinetics compartmental model. Comparisons do not find significant differences between the right and left livers nor between models A, B, C and D for uptake time constants nor between models A, B and D for excretion time constants.

Conclusion

Reference values of hepatobiliary function in normal CD1 mice were obtained using different compartmental models.

The university hospital of Lille and the Lille school of medicine always encouraged the development of tehcnological researches. For this purpose, they supported technological research and innovation with the ITM, but also technological evaluation with « Evalab », the first « usability lab » in Europe dedicated to the assessment of healthcare computer applications. This research closely associates searchers, companies, but also the future users (mainly healthcare professionals but also patients) to improve the acceptability of the systems, to avoid errors and adverse effects and increase the patient's safety.

Due to a short half-life, growth factors, which could be used as therapeutic agents, usually require carriers able to protect their biological activity and to sustain protein delivery in situ. It was shown that water-soluble functionalized dextrans (DMCB) present some heparin-like properties, especially the capacity to interact with Heparin-Binding Growth Factors such as TGFβ₁. In this study, dextran-based hydrogels were synthesized by cross-linking a DMCB with native dextrans to develop a TGFβ₁ release carrier. In vivo, these hydrogels were demonstrated to be biocompatible and to allow good cell invasion. In vitro, functionalized hydrogels retained 50 to 60% of initially loaded growth factor contrary to unfunctionalized hydrogels. The degree of hydrogel functionalization did not influence the amount of entrapped growth factor. Nevertheless, it modulated the prominent type of interactions between TGFβ₁ and hydrogels.

Located at the interface between fundamental research and clinical research the CIT of Bordeaux associates the industrialists, the University Hospital environment in order to develop biomedical research in the field of biomaterials. Thus the CIT of Bordeaux brings a methodological support in the phases of design, development, evaluation which the projects are upstream marking CE, relate to marking CE or are downstream from marking CE. The three axes of CIT biomaterials activities are in vitro preclinic research, preclinic research in vivo and clinical research (clinical studies control). “The study of the modification of the coral by heat treatments and supercritical fluid for its improvement as osseous substitute in odontology and orthopaedic” is a typical project of the CIT biomaterials of Bordeaux. The objective of this project was to evaluate the effectiveness of various innovating techniques of extracellular matrix extraction and their effect on biocompatibility and mechanical performances of the coral, in order to obtain a natural osseous substitute entirely mineral.

The home healthcare of fragile or elderly people may be facilitated by IC technologies in order to monitor continuously the health level and to assist professionals, physicians and social workers. The assessment of the level of autonomy is one of the main indicators for adapting the level of care. The standardized tools (AGGIR or ADL) evaluate the capacities to perform autonomously the daily activities. These are user dependant. This paper presents scientific works on acquisition and interpretation of the signals derived from accelerometers in a Micro system, called “actimometer”. It can be embedded in the underwear to monitor continuously the level of activity, the capacities to displacements and transfers. The interpretation is so accurate to classify between different kinds of transfers in an ecological scenario. Results show that accelerometers data reproducibility depend on the two criteria: velocity and subject. In addition, a new method is able to differentiate BtS (Back-to-Sit) and StS (Sit-to-Stand) at the three velocities with a overall sensitivity and specificity equal to 95.32 and 96.10% respectively. Finally, the transition duration is determined for all trials.