Journal of Holistic Integrative Pharmacy最新文献

{"title":"Study on extraction technology and antioxidant activity of \"Sibai\" extracts based on orthogonal design and Caenorhabditis elegans","authors":"Zhenghao Chen ,&nbsp;Haoxian Chen ,&nbsp;Xiaohua Ye ,&nbsp;Ping Zhao ,&nbsp;Yan Wang","doi":"10.1016/j.jhip.2025.11.006","DOIUrl":"10.1016/j.jhip.2025.11.006","url":null,"abstract":"<div><h3>Objective</h3><div>This study examined the antioxidant, whitening, anti-aging, and safety properties of \"Sibai\" extract obtained from <em>Bombyx Batryticatus</em>, <em>Ampelopsis japonica</em>, <em>Radix Paeoniae Alba</em>, and <em>Atractylodes macrocephala</em>.</div></div><div><h3>Methods</h3><div>The \"Sibai\" extract was optimized by orthogonal design and evaluated for in vitro antioxidant activity through hydroxyl radical scavenging, DPPH scavenging, and total reducing capacity measurements. Tyrosinase inhibition and hemolysis assays assessed its whitening potential and biosafety. Anti-aging effects were examined using <em>Caenorhabditis elegans (C. elegans</em>) lifespan and fecundity assays, while antioxidant capacity under H₂O₂, juglone, and heat stress was evaluated. Motor function was analyzed via head thrashing, body bending, and spontaneous locomotion tests. Finally, aging-related changes in lipofuscin, reactive oxygen species (ROS), malondialdehyde (MDA), and catalase (CAT) levels were measured.</div></div><div><h3>Results</h3><div>The optimal ratio of <em>Bombyx Batryticatus</em>, <em>Ampelopsis japonica</em>, <em>Radix Paeoniae Alba</em>, and <em>Atractylodes macrocephala</em> rhizome was determined to be 3:3:1:1. At specific concentrations, the extract demonstrated significant biological activity: the scavenging rate for hydroxyl radicals was 61.70% (at 48 ​mg/mL), the scavenging rate for DPPH radicals was 93.53% (at 4 ​mg/mL), and its total reducing power at 48 ​mg/mL was comparable to that of Vitamin C (Vc). The tyrosinase inhibition rate was 74.90% (at 24 ​mg/mL), and the hemolysis rate remained below 15%, indicating good in vitro safety. In lifespan experiments using <em>C. elegans</em> as a model, treatment with 48 ​mg/mL extract reduced fecundity and extended the normal lifespan by 38.5%. Under oxidative stress induced by H₂O₂ and juglone, as well as under heat stress, the survival time of <em>C. elegans</em> in the 48 ​mg/mL treatment group increased by 37.9%, 69.5%, and 49.2% respectively. In terms of motor ability, the 48 ​mg/mL treatment group showed increases in head thrashing and body bending frequencies by 61.4% and 220.7% respectively, along with excellent autonomous movement capacity. At the cellular level, levels of lipofuscin, ROS, and MDA were reduced by 65.8%, 41.5%, and 51.3% respectively, while CAT activity increased by 329.1%.</div></div><div><h3>Conclusion</h3><div>This study employed an orthogonal experimental design to determine the optimal formulation. Subsequent validation confirmed that the optimized extract possessed remarkable antioxidant and whitening properties. Furthermore, it was found to significantly enhance stress resistance in <em>C. elegans</em> and improve age-related phenotypes. These results indicated that this formulation may effectively address issues related to aging and oxidative stress.</div></div>","PeriodicalId":100787,"journal":{"name":"Journal of Holistic Integrative Pharmacy","volume":"6 4","pages":"Pages 398-406"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145797965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A key pathological change in early stage ankylosing spondylitis: cartilage dyshomeostasis induced by Osteogenic Progenitor Cells","authors":"Yuxin Cai ,&nbsp;Liyan Li ,&nbsp;Xiaoyan Du ,&nbsp;Jun Chen ,&nbsp;Kai-Kei Miu ,&nbsp;Yujie Deng ,&nbsp;Li Lu","doi":"10.1016/j.jhip.2025.11.002","DOIUrl":"10.1016/j.jhip.2025.11.002","url":null,"abstract":"<div><h3>Objective</h3><div>Ankylosing spondylitis (AS) is a chronic inflammatory disease primarily affecting the spine and sacroiliac joints, with symptoms of back pain and stiffness partially explained by pathophysiological events encompassing enthesitis, ectopic bone formation, and cartilage degeneration. Progressive fusion of vertebrae leading to reduced mobility is a prominent character of AS, but there remain many unknowns regarding bone pathology, in particular abnormal bone outgrowth. This study aims to elucidate the cellular and molecular mechanisms that underpin pathological osteogenesis in the AS.</div></div><div><h3>Methods</h3><div>We conducted a 3-year longitudinal study on cynomolgus monkeys with spontaneous ankylosing spondylitis, which included performing imaging examinations, H&amp;E, Masson, IHC staining, and bone histomorphometric analysis. These methods were employed to clarify the pathological features of different stages of AS and to explore the pathological mechanisms leading to pathological ossification.</div></div><div><h3>Results</h3><div>In the spines of AS-afflicted cynomolgus monkeys, early pathological features included inflammatory infiltration, invasion of cartilage endplates by fibrous-rich granulation tissue, and erosion cavities in the cartilage endplates, all of which were highly spatiotemporally correlated with the formation of ectopic new bone. After acute inflammation, fibroblasts in the granulation tissue underwent osteoblastic differentiation to form woven bone, which was presumed as the primary process of syndesmophyte formation in AS. The appearance of erosion cavities in cartilage endplates marked the onset of disrupted endplate homeostasis. Pathological reductions were observed in the thickness of the ligaments, subchondral bone plates, hypertrophic cell layers, and intervertebral disc height within the lumbar joints. In the later stages, ectopic cartilage ossification and ectopic fibrous ossification together contributed to syndesmophyte formation, such that the cartilage endplate almost completely disappeared, leading to joint ankylosis. At the cell level, accumulated cells within the early cartilage endplate erosion cavities were confirmed to be bone marrow-derived osteoprogenitor cells, whereas the abnormal expression of BMP-2 signaling in the locality promoted bone remodeling in AS.</div></div><div><h3>Conclusion</h3><div>This study provides a pathological analysis of spinal joints in AS-afflicted cynomolgus monkeys, summarizing the pathological features during the progression of AS. It demonstrates that bone marrow-derived osteoprogenitor cells initiate cartilage ossification and likely revealed an unknown mechanism for AS ossification. These findings offer new insights into the skeletal pathology of AS to inform future direction for devising therapeutic approaches to combat AS.</div></div>","PeriodicalId":100787,"journal":{"name":"Journal of Holistic Integrative Pharmacy","volume":"6 4","pages":"Pages 491-503"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145840728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Banxia Shumi decoction targets ribosome biogenesis to alleviate insomnia","authors":"Xijian Liu,&nbsp;Xiangye Gao,&nbsp;Suyu Hou,&nbsp;Dandan Luo,&nbsp;Yan Zhang","doi":"10.1016/j.jhip.2025.11.001","DOIUrl":"10.1016/j.jhip.2025.11.001","url":null,"abstract":"<div><h3>Objective</h3><div>This study aims to investigate the novel mechanism by which Banxia Shumi Decoction (BXSM) alleviates insomnia through the regulation of ribosome biogenesis (Ribosis).</div></div><div><h3>Methods</h3><div>We identified the molecular targets associated with the components of BXSM utilizing multiple databases, including PharmMapper and SwissTargetPrediction. Furthermore, we analyzed differentially expressed genes (DEG) linked to insomnia from the GSE 208668 dataset and compiled genes associated with Ribosis from existing literature. A protein interaction network was then constructed to pinpoint the central hub gene related to Ribosis. Additionally, various analytical approaches were employed, including Gene Set Enrichment Analysis (GSEA), Gene Ontology (GO) analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis, and immune infiltration analysis, to explore the relevant functional implications of the identified genes. The validations were performed using operating characteristic (ROC) analysis, Gene Expression Omnibus dataset, quantitative real-time polymerase chain reaction (qRT-PCR), molecular docking, and molecular dynamics simulation.</div></div><div><h3>Results</h3><div>KAT2B was recognized as the key target, daidzin, naringin, aescin, kaempferol, and apigenin bind stably with KAT2B. Notably, KAT2B was primarily involved in various pathways, including the thyroid hormone signaling pathway. ROC assessment indicated that KAT2B exhibited high accuracy in distinguishing individuals with insomnia. Compared with the model group, BXSM could significantly upregulate the mRNA expression of KAT2B, and downregulate the mRNA expression level of THRB, MYC, and PGC-1α (<em>P</em> ​&lt; ​0.01).</div></div><div><h3>Conclusion</h3><div>This study elucidates the potential mechanisms by which BXSM ameliorates insomnia through its core bioactive compounds (daidzin, naringin, aescin, kaempferol, and apigenin). These compounds target KAT2B to modulate the thyroid hormone signaling pathway, which in turn regulates Ribosis.</div></div>","PeriodicalId":100787,"journal":{"name":"Journal of Holistic Integrative Pharmacy","volume":"6 4","pages":"Pages 373-388"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145652047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of solvent composition, concentration, and temperature on the solubility of naproxen: A review of mono-solvent, binary, and ternary systems","authors":"Abdolhamid Khodadadi ,&nbsp;Shirin Boghrat ,&nbsp;Zahra Besharati ,&nbsp;Seyed Hossein Hashemi","doi":"10.1016/j.jhip.2025.12.004","DOIUrl":"10.1016/j.jhip.2025.12.004","url":null,"abstract":"<div><div>Solubility is a key factor affecting drug absorption and therapeutic effectiveness. Naproxen, a common anti-inflammatory medication, has poor water solubility, which limits its bioavailability. This review thoroughly explores various solvent-based approaches to overcome this issue. It discusses mono-solvent systems (acetone, chloroform, β-cyclodextrins), binary systems (ethanol, PEG, PG, deep eutectic solvents), and ternary systems that combine water with co-solvents and additives like HPβCD and L-arginine. The review also assesses advanced techniques such as supercritical CO₂ and CO₂-expanded solvents. Findings show that solvent type, hydrogen bonding ability, and molecular interactions play crucial roles in improving solubility. Ternary systems exhibit notable synergistic effects, and deep eutectic solvents present promising eco-friendly alternatives. In conclusion, careful selection and combination of solvents can significantly enhance naproxen's solubility, guiding future research toward optimizing formulations for pharmaceutical and industrial uses.</div></div>","PeriodicalId":100787,"journal":{"name":"Journal of Holistic Integrative Pharmacy","volume":"6 4","pages":"Pages 427-442"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145799733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A review on the extraction, separation, purification, detection of flavonoids from aging Citri Reticulatae Pericarpium and its beneficial effects on spleen deficiency","authors":"Leixi Ye ,&nbsp;Le Yu ,&nbsp;Hongjie Xu ,&nbsp;Kunyi Liu ,&nbsp;Yunqi Shan ,&nbsp;Quan Yang ,&nbsp;Xiaoming Sun","doi":"10.1016/j.jhip.2025.12.001","DOIUrl":"10.1016/j.jhip.2025.12.001","url":null,"abstract":"<div><div>Citri Reticulatae Pericarpium (CRP) plays beneficial effects on regulating Qi and strengthening spleen, drying dampness, and resolving phlegm in traditional Chinese medicine. This review centered on the advantageous impacts of flavonoids regarding spleen deficiency as well as their extraction, separation, and purification procedures, aiming to disclose the characteristics manifested in spleen deficiency conditions. The existing literature has not merely shed light on the therapeutic mechanisms underlying CRP flavonoids within the digestive tract, but has also highlighted their cooperative and amplifying impacts on other organs. By integrating these diverse strands of research, a more in-depth understanding of the pharmacological actions of CRP has emerged, providing a fresh theoretical framework for deciphering the scientific significance of aged CRP.</div></div>","PeriodicalId":100787,"journal":{"name":"Journal of Holistic Integrative Pharmacy","volume":"6 4","pages":"Pages 415-426"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145797966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An integrative framework for precision medication in hypertension: Applying a dialectical perspective to traditional Chinese and Western medicine","authors":"Jinliang Liu ,&nbsp;Jiena Xiao ,&nbsp;Zhongwei Lin ,&nbsp;Mingzhu Xiao","doi":"10.1016/j.jhip.2025.09.001","DOIUrl":"10.1016/j.jhip.2025.09.001","url":null,"abstract":"<div><div>Hypertension is the most common cardiovascular disease. In terms of diagnosis and treatment, Traditional Chinese Medicine (TCM) and Western medicine each have their own strengths, but also have certain limitations. Affected by the combined effects of genetic and environmental factors, the pharmacotherapy of hypertension shows significant individual differences. Therefore, how to integrate the advantages of TCM and Western medicine to achieve precision medication has become an important research topic. A single diagnostic and therapeutic model is difficult to take into account both the characteristics of TCM's \"holistic regulation\" and Western medicine's \"precision intervention\": TCM focuses on holistic syndrome differentiation and the restoration of bodily balance, yet it is relatively vague in the analysis of disease mechanisms and the identification of drug targets; Western medicine excels in targeted therapy, but often overlooks the patient's overall physiological state and the holistic progression of the disease. In the current development of precision medication, the concept of integrative pharmacy provides important support. By systematically integrating multi-source knowledge and technical means, it is committed to combining the macro-holistic perspective with micro-precision intervention, and provides a practical framework for the integration and innovation of TCM and Western medicine. Against this background, TCM takes \"holistic concept\" and \"syndrome differentiation and treatment\" as its fundamentals, and devotes itself to maintaining the dynamic balance of human body functions; Western medicine, relying on advanced tools such as genomics and proteomics, emphasizes the precise identification of etiologies and targeted therapy. The two show a dialectical unity in concepts and methods, and jointly serve the realization of the goal of individualized treatment for hypertension. With \"principle, method, prescription, and medicine\" as the logical main line, this paper systematically explores the diagnostic and therapeutic ideas and practical strategies of TCM and Western medicine for hypertension, and expounds on how to take integrative pharmacy as a bridge to incorporate the holistic thinking of TCM into the modern precision medication system, thereby providing theoretical references and practical paths for promoting the integrated diagnosis and treatment of hypertension with TCM and Western medicine and the implementation of precise drug intervention.</div></div>","PeriodicalId":100787,"journal":{"name":"Journal of Holistic Integrative Pharmacy","volume":"6 3","pages":"Pages 290-300"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145120794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrative network pharmacology and transcriptomics suggest the mechanism of Buyang Huanwu Decoction in attenuating vascular aging via AMPK signaling pathway","authors":"Xuejuan Shen ,&nbsp;Jieyong Du ,&nbsp;Xiangyu Chen ,&nbsp;Xiaodie Chen ,&nbsp;Nan Xiao ,&nbsp;Danping Huang ,&nbsp;Yubin Yang ,&nbsp;Rong Chen ,&nbsp;Zunpeng Shu ,&nbsp;Li Zhang","doi":"10.1016/j.jhip.2025.09.004","DOIUrl":"10.1016/j.jhip.2025.09.004","url":null,"abstract":"<div><h3>Objective</h3><div>Vascular aging is a critical factor in cardiovascular diseases. Buyang Huanwu Decoction (BYHWD), a traditional Chinese medicine, shows potential in delaying vascular aging, yet its mechanisms remain unclear. This study aims to explore BYHWD's pharmacological mechanisms through integrative network pharmacology and transcriptomics.</div></div><div><h3>Methods</h3><div>Network pharmacology identified BYHWD targets related to aging, followed by pathway enrichment analysis. Vascular histopathology was evaluated using HE, Masson, and EVG staining. Senescence biomarkers SA-<em>β</em>-gal, advanced glycation end products (AGEs), p16, p21, and p53 were determined. Oxidative-inflammatory markers were quantified via ELISA and RT-qPCR. Transcriptomic profiling used Illumina NovaSeq. AMP-activated protein kinase (AMPK) signaling pathway analysis employed immunohistochemistry.</div></div><div><h3>Results</h3><div>Network pharmacology identified 103 key targets, including SIRT1 and AMPK, related to oxidative stress and inflammation. In the D-galactose-induced aging model rats, BYHWD treatment significantly reduced vascular media thickness by 28% in the medium-dose group, collagen deposition, and markers of senescence, such as SA-<em>β</em>-gal, AGEs, p16, p21, and p53. Transcriptomics revealed AMPK signaling pathway enrichment, and further experiments revealed that BYHWD mediates its effects involving activation of the AMPK/Sirt1/Foxo3a axis, a key regulator of vascular homeostasis.</div></div><div><h3>Conclusion</h3><div>BYHWD may attenuate vascular aging by AMPK-mediated regulation of oxidative stress and inflammation, providing a novel multi-target strategy against age-related cardiovascular diseases. The underlying molecular mechanism still needs to be further clarified.</div></div>","PeriodicalId":100787,"journal":{"name":"Journal of Holistic Integrative Pharmacy","volume":"6 3","pages":"Pages 276-289"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145120856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mendelian randomization analysis establishes a causal relationship between COVID-19 and cardiometabolic diseases","authors":"Haibo Chen ,&nbsp;Lizhen Liao ,&nbsp;Zezhi Ke ,&nbsp;Xu Zhang ,&nbsp;Xiaodong Zhuang ,&nbsp;Xin Gao ,&nbsp;Litao Pan","doi":"10.1016/j.jhip.2025.08.001","DOIUrl":"10.1016/j.jhip.2025.08.001","url":null,"abstract":"<div><h3>Objective</h3><div>The causal impacts of COVID-19 on cardiometabolic diseases remained uncertain. This study utilized the two-sample Mendelian randomization (MR) method to evaluate causal relationships between COVID-19 (susceptibility and severity) and four primary cardiometabolic diseases (type 2 diabetes, coronary heart disease, ischemic stroke, and heart failure).</div></div><div><h3>Methods</h3><div>MR analysis was conducted using genome-wide association study (GWAS) results. Susceptibility and severity were defined as COVID-19-positive and COVID-19-hospitalization, respectively. Data from the COVID-19 Host Genetics Initiative were used for susceptibility and severity analysis. Consortium data from Spracklen CN, Nikpay, Malik R, and Neale lab were employed for type 2 diabetes, coronary heart disease, ischemic stroke, and heart failure, respectively.</div></div><div><h3>Results</h3><div>For COVID-19 susceptibility, the inverse variance weighted (IVW) method showed the odds ratio (OR) (95% confidence interval [CI], <em>P</em>-value) for type 2 diabetes was 1.719 (1.510–1.956, <em>P</em> ​= ​0.000). For COVID-19 severity, the IVW method estimate indicated that the OR (95% CI, P-value) for ischemic stroke was 1.051 (1.008–1.095, <em>P</em> ​= ​0.020). Moreover, the OR for heart failure was slightly higher in the hospitalized population than in the control population (1.001, 95% CI 1.000–1.002, <em>P</em> ​= ​0.010). The remaining results were negative.</div></div><div><h3>Conclusion</h3><div>This MR study establishes that genetically predicted COVID-19 susceptibility causally increases type 2 diabetes risk, while severe infection shows suggestive causal links with ischemic stroke and heart failure, redefining COVID-19 as an independent cardiometabolic risk factor.</div></div>","PeriodicalId":100787,"journal":{"name":"Journal of Holistic Integrative Pharmacy","volume":"6 3","pages":"Pages 301-307"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145120795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unlocking artemisinin's potential in polycystic ovary syndrome treatment: A call for controlled human trials","authors":"Osama Ahmad ,&nbsp;Komal Zulfiqar","doi":"10.1016/j.jhip.2025.09.009","DOIUrl":"10.1016/j.jhip.2025.09.009","url":null,"abstract":"","PeriodicalId":100787,"journal":{"name":"Journal of Holistic Integrative Pharmacy","volume":"6 3","pages":"Pages 322-323"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145219991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization, characterization, hypoglycemic and antioxidant activity studies of ultrasonic-assisted deep eutectic solvent extraction of polysaccharides from Perilla frutescens","authors":"Jiajian Tang,&nbsp;Zilin Cong,&nbsp;Huijia Dai,&nbsp;Sigui Zhou,&nbsp;Yao Wen","doi":"10.1016/j.jhip.2025.09.008","DOIUrl":"10.1016/j.jhip.2025.09.008","url":null,"abstract":"<div><h3>Objective</h3><div>In order to further develop and utilise <em>Perilla frutescens</em> polysaccharides, the aim of this study was to optimize process conditions for the extraction of <em>Perilla frutescens</em> polysaccharides with deep eutectic solvents (DES), to investigate their biological activities and to analyse their monosaccharide composition.</div></div><div><h3>Methods</h3><div><em>Perilla frutescens</em> polysaccharides were extracted by DES and ultrasonic extraction, and the optimal extraction process was obtained by one-way test and response surface method. The extract was collected and concentrated, alcohol precipitated, deproteinized, dialyzed, and evaporated to make a solution, which was purified by DEAE and Sephadex G-75 columns to obtain the arginal polysaccharide PFP-1.</div></div><div><h3>Results</h3><div>The polysaccharide was extracted from <em>Perilla frutescens</em> by ultrasonication with the aid of a new type of DES, and the optimal conditions for extraction were determined: the optimal DES system for extracting the polysaccharide of <em>Perilla frutescens</em> and the molar ratio was choline chloride: oxalic acid ​= ​2:1; ultrasonication temperature, power, and time were 70 ​°C, 280 ​W, and 45 ​min, respectively; and the liquid-solid ratio was 21 ​mL/g, and the extraction rate of polysaccharide was 2.44%. The molecular weight (<em>Mw</em>) of PFP-1 is approximately 11600 ​Da. Hydrolysis and derivatization of the refined polysaccharide PFP-1 followed by analytical experiments determined that the polysaccharide monosaccharides consisted of rhamnose, glucuronic acid, and glucose, with the molar ratios of 1.45: 2.82: 1.41. The antioxidant activity and hypoglycemic activity of the polysaccharide were determined to be good by free radical scavenging and enzyme inhibition experiments, and the IC₅₀ of <em>Perilla frutescens</em> polysaccharide against DPPH• was 0.117 ​mg/mL and that against <em>α</em>-amylase was 0.002 ​mg/mL.</div></div><div><h3>Conclusion</h3><div>This study can provide an experimental basis for the establishment of an industrialized production process of polysaccharides and the study of their biological activities.</div></div>","PeriodicalId":100787,"journal":{"name":"Journal of Holistic Integrative Pharmacy","volume":"6 3","pages":"Pages 333-342"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145265019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}