Pub Date : 2024-12-01DOI: 10.1016/j.jhip.2024.11.006
Jiajun Zhu , Yuanru Zheng , Yanhui Ge
The medicinal value of Aloe vera (AV) has been continuously explored and utilized in recent years, becoming a research hotspot. Some basic studies have found that AV had cosmetic effects such as whitening, sun protection, antioxidant, anti-aging, and moisturizing. Some clinical trials have found that AV had medical effects such as antibacterial, anti-damage, and promoting wound healing. In this work, we summarized the indications and therapeutic efficacy of AV in cosmetology and clinical treatment of dermatosis, in order to provide certain inspiration for the development of new AV-based skincare products and new medicines for skin diseases.
{"title":"Study on the application of Aloe vera in cosmetology and clinical treatment of skin diseases","authors":"Jiajun Zhu , Yuanru Zheng , Yanhui Ge","doi":"10.1016/j.jhip.2024.11.006","DOIUrl":"10.1016/j.jhip.2024.11.006","url":null,"abstract":"<div><div>The medicinal value of <em>Aloe vera</em> (AV) has been continuously explored and utilized in recent years, becoming a research hotspot. Some basic studies have found that AV had cosmetic effects such as whitening, sun protection, antioxidant, anti-aging, and moisturizing. Some clinical trials have found that AV had medical effects such as antibacterial, anti-damage, and promoting wound healing. In this work, we summarized the indications and therapeutic efficacy of AV in cosmetology and clinical treatment of dermatosis, in order to provide certain inspiration for the development of new AV-based skincare products and new medicines for skin diseases.</div></div>","PeriodicalId":100787,"journal":{"name":"Journal of Holistic Integrative Pharmacy","volume":"5 4","pages":"Pages 299-304"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143174793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-01DOI: 10.1016/j.jhip.2024.12.001
Huiqun Tian, Li Chen
The treatment of fibrosis faces a significant challenge due to the lack of effective therapies that can reverse established fibrosis. Early detection is vital for intervention, yet distinguishing fibrosis from normal tissue repair is complex. Human Epididymis Protein 4 (HE4), a traditional tumor marker, has been found to be increased in some non-neoplastic conditions, such as fibrosis related diseases. According to properties analysis, HE4 has been characterized as a highly stable cross-class protease inhibitor, which interacts with key fibrotic proteins (such as MMP2 and PRSS family members) and potentially involves in the progression of fibrosis by inhibiting the enzymatic activity of these proteins. Meanwhile, studies indicated that HE4 may be involved in fibrosis through PI3K/AKT, NF-κB, MAPK, and other signaling pathways. Here we summarized the latest research progress of HE4 in pulmonary fibrosis, renal fibrosis, myocardial fibrosis, liver fibrosis, and autoimmune diseases induced fibrosis. As reported in this review, HE4 was closely related to disease severity and prognosis, and also was a promising prognostic evaluation marker and therapeutic intervention target for fibrotic diseases.
{"title":"Human Epididymis Protein 4 (HE4) as a promising biomarker and therapy target in fibrotic diseases: A review","authors":"Huiqun Tian, Li Chen","doi":"10.1016/j.jhip.2024.12.001","DOIUrl":"10.1016/j.jhip.2024.12.001","url":null,"abstract":"<div><div>The treatment of fibrosis faces a significant challenge due to the lack of effective therapies that can reverse established fibrosis. Early detection is vital for intervention, yet distinguishing fibrosis from normal tissue repair is complex. Human Epididymis Protein 4 (HE4), a traditional tumor marker, has been found to be increased in some non-neoplastic conditions, such as fibrosis related diseases. According to properties analysis, HE4 has been characterized as a highly stable cross-class protease inhibitor, which interacts with key fibrotic proteins (such as MMP2 and PRSS family members) and potentially involves in the progression of fibrosis by inhibiting the enzymatic activity of these proteins. Meanwhile, studies indicated that HE4 may be involved in fibrosis through PI3K/AKT, NF-κB, MAPK, and other signaling pathways. Here we summarized the latest research progress of HE4 in pulmonary fibrosis, renal fibrosis, myocardial fibrosis, liver fibrosis, and autoimmune diseases induced fibrosis. As reported in this review, HE4 was closely related to disease severity and prognosis, and also was a promising prognostic evaluation marker and therapeutic intervention target for fibrotic diseases.</div></div>","PeriodicalId":100787,"journal":{"name":"Journal of Holistic Integrative Pharmacy","volume":"5 4","pages":"Pages 291-298"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143174789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-01DOI: 10.1016/j.jhip.2024.11.001
Yixiang Wu , Xiaoshan Zheng , Youchaou Mobet , Huiqun Tian , Fangsen Li , Huan Li , Lifeng Xie , Yanyue Deng , Xiaodi Zhu , Chuxi Tang , Hongwei Shao , Song Liu
Burn is a suddenly occurring injury. The healing process of burn is complex. Aloe vera (L.) Burm.f. (Av) is widely used as Chinese folk medicine for the treatment of burn. While effective pharmacological components, role and mechanism of Av in the treatment of burn remain to be fully elucidated. Arachidonic Acid (AA) and Quercetin (QUE) are identified as active components of Av for the treatment of burn by network pharmacology analysis and experimental verification. Animal experiments’ results showed that AA and QUE could shorten the burn wound healing time. The expression of inflammatory factor TNF-α, MyD88 and P-NF-κB p65 were down-regulated and the ER (ESR1), SRC were up-regulated in AA treated NIH 3T3 cells. The expression of MyD88 was downregulated in QUE treated NIH 3T3 cells. The Av promotes burn healing may be via the anti-inflammatory effect and regulation on NF-κB signal pathway or estrogen signal pathway by its active components AA and QUE. This research may construct a bridge between Av and burn wounds, and skin therapy agent exploration.
{"title":"Research on the role and mechanism of Aloe vera (L.) Burm.f. in the treatment of burn: Based on network pharmacology analysis and experimental verification","authors":"Yixiang Wu , Xiaoshan Zheng , Youchaou Mobet , Huiqun Tian , Fangsen Li , Huan Li , Lifeng Xie , Yanyue Deng , Xiaodi Zhu , Chuxi Tang , Hongwei Shao , Song Liu","doi":"10.1016/j.jhip.2024.11.001","DOIUrl":"10.1016/j.jhip.2024.11.001","url":null,"abstract":"<div><div>Burn is a suddenly occurring injury. The healing process of burn is complex. <em>Aloe vera</em> (L.) Burm.f. (Av) is widely used as Chinese folk medicine for the treatment of burn. While effective pharmacological components, role and mechanism of Av in the treatment of burn remain to be fully elucidated. Arachidonic Acid (AA) and Quercetin (QUE) are identified as active components of Av for the treatment of burn by network pharmacology analysis and experimental verification. Animal experiments’ results showed that AA and QUE could shorten the burn wound healing time. The expression of inflammatory factor TNF-α, MyD88 and P-NF-κB p65 were down-regulated and the ER (ESR1), SRC were up-regulated in AA treated NIH 3T3 cells. The expression of MyD88 was downregulated in QUE treated NIH 3T3 cells. The Av promotes burn healing may be via the anti-inflammatory effect and regulation on NF-κB signal pathway or estrogen signal pathway by its active components AA and QUE. This research may construct a bridge between Av and burn wounds, and skin therapy agent exploration.</div></div>","PeriodicalId":100787,"journal":{"name":"Journal of Holistic Integrative Pharmacy","volume":"5 4","pages":"Pages 262-276"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143174790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-01DOI: 10.1016/j.jhip.2024.11.003
Wanying He , Yaying Yu , Hui He , Qian Huang , Zhiting Liu , Fan Yang , Lin Zhou
Nonalcoholic fatty liver disease (NAFLD) is an escalating global health issue, leading to liver fat accumulation, inflammation, fibrosis, and potential liver failure. In our preliminary network pharmacological study, it was hypothesized that Pedalitin (PED), a flavonoid found in black sesame plants, might exhibit protective effects against NAFLD. However, the effects and mechanisms of PED underlying its action on NAFLD are not yet fully understood. This study aimed to explore the potential effects and mechanisms of PED on NAFLD using a combination of network pharmacology, molecular docking, and the LO2 cell model. Potential targets for PED and NAFLD were predicted through public databases. Protein-protein interaction (PPI) networks were constructed, and Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were performed. Molecular docking was used to predict the target genes that could bind to PED. In vitro experiments using the LO2 cell model indicated that PED significantly reduced TG level (p < 0.05) and the formation of lipid droplets. The expression levels of key factors in fatty acid metabolism (CPT2, HADH), inflammatory factors (IL-17, TNF-α), and the FOXO signaling pathway (EGFR, IRS1, AKT1, FOXO1) were significantly downregulated in LO2 cells treated with PED (p < 0.05). In conclusion, PED may modulate local lipid metabolism and mitigate inflammatory responses through the FOXO signaling pathway.
{"title":"Pedalitin could regulate lipid metabolism and attenuate inflammatory factors in a non-alcoholic fatty liver disease cell model","authors":"Wanying He , Yaying Yu , Hui He , Qian Huang , Zhiting Liu , Fan Yang , Lin Zhou","doi":"10.1016/j.jhip.2024.11.003","DOIUrl":"10.1016/j.jhip.2024.11.003","url":null,"abstract":"<div><div>Nonalcoholic fatty liver disease (NAFLD) is an escalating global health issue, leading to liver fat accumulation, inflammation, fibrosis, and potential liver failure. In our preliminary network pharmacological study, it was hypothesized that Pedalitin (PED), a flavonoid found in black sesame plants, might exhibit protective effects against NAFLD. However, the effects and mechanisms of PED underlying its action on NAFLD are not yet fully understood. This study aimed to explore the potential effects and mechanisms of PED on NAFLD using a combination of network pharmacology, molecular docking, and the LO2 cell model. Potential targets for PED and NAFLD were predicted through public databases. Protein-protein interaction (PPI) networks were constructed, and Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were performed. Molecular docking was used to predict the target genes that could bind to PED. <em>In vitro</em> experiments using the LO2 cell model indicated that PED significantly reduced TG level (<em>p</em> < 0.05) and the formation of lipid droplets. The expression levels of key factors in fatty acid metabolism (CPT2, HADH), inflammatory factors (IL-17, TNF-α), and the FOXO signaling pathway (EGFR, IRS1, AKT1, FOXO1) were significantly downregulated in LO2 cells treated with PED (<em>p</em> < 0.05). In conclusion, PED may modulate local lipid metabolism and mitigate inflammatory responses through the FOXO signaling pathway.</div></div>","PeriodicalId":100787,"journal":{"name":"Journal of Holistic Integrative Pharmacy","volume":"5 4","pages":"Pages 314-322"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143174794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-01DOI: 10.1016/j.jhip.2024.11.005
Ran Xiong , Hui Zhang , Yueyun Li , Guihao Zeng , Yonghui Liu , Yeyou Xu
Objective
This study analyzes the European Union's (EU's) regulatory practices of Herbal Medicinal Products (HMPs), aiming to provide implications for Traditional Chinese Medicines (TCMs) registration process within the Guangdong-Hong Kong-Macao Greater Bay Area (GBA).
Methods
This paper conducts a comparative analysis of the regulatory agencies, legal frameworks, registration classifications, market authorization procedures, and the number of applications and approvals for both HMPs in the EU and TCMs in the GBA, thereby identifying the distinctive regulatory features across these regions.
Results
Our study finds that the EU has established a scientific, efficient, and harmonized regulation system for HMPs, whereas the registration of TCMs in the GBA is characterized by complexity and jurisdictional disparities in requirements and procedures. Drawing on the EU's advanced regulatory practices, the study offers recommendations to enhance the mutual recognition and accessibility of TCMs within the GBA.
Conclusion
This study offers a comprehensive understanding of the EU's HMPs regulation and the GBA's TCMs registration, contributing to the synergistic development of TCMs in the GBA.
{"title":"Enlightenment of EU Herbal Medicinal Products regulation on the registration of Traditional Chinese Medicines in the Guangdong-Hong Kong-Macao Greater Bay Area","authors":"Ran Xiong , Hui Zhang , Yueyun Li , Guihao Zeng , Yonghui Liu , Yeyou Xu","doi":"10.1016/j.jhip.2024.11.005","DOIUrl":"10.1016/j.jhip.2024.11.005","url":null,"abstract":"<div><h3>Objective</h3><div>This study analyzes the European Union's (EU's) regulatory practices of Herbal Medicinal Products (HMPs), aiming to provide implications for Traditional Chinese Medicines (TCMs) registration process within the Guangdong-Hong Kong-Macao Greater Bay Area (GBA).</div></div><div><h3>Methods</h3><div>This paper conducts a comparative analysis of the regulatory agencies, legal frameworks, registration classifications, market authorization procedures, and the number of applications and approvals for both HMPs in the EU and TCMs in the GBA, thereby identifying the distinctive regulatory features across these regions.</div></div><div><h3>Results</h3><div>Our study finds that the EU has established a scientific, efficient, and harmonized regulation system for HMPs, whereas the registration of TCMs in the GBA is characterized by complexity and jurisdictional disparities in requirements and procedures. Drawing on the EU's advanced regulatory practices, the study offers recommendations to enhance the mutual recognition and accessibility of TCMs within the GBA.</div></div><div><h3>Conclusion</h3><div>This study offers a comprehensive understanding of the EU's HMPs regulation and the GBA's TCMs registration, contributing to the synergistic development of TCMs in the GBA.</div></div>","PeriodicalId":100787,"journal":{"name":"Journal of Holistic Integrative Pharmacy","volume":"5 4","pages":"Pages 344-353"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143174700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-01DOI: 10.1016/j.jhip.2024.12.003
Jing Zhang , Guobin Zheng , Shangjing Liu , Yaodong Miao , Shu Yang , Sheng Li , Zhihui Yang , Danping Zhuo , Rui Guo , Yang Guo , Rongjiang Shao , Yunqing Hua , Chuanrui Ma
Background
Prolonged bradycardia leads to inadequate pumping of the heart, causing myocardial ischemia, which in turn affects cardiac function. Depending on its clinical features, improving the AV node conduction block may be an important tool to inhibit the progression of the disease. Lingbao Huxin Dan, which consists of several traditional Chinese medicines, is used for conditions similar to bradycardia and may be a potential therapeutic agent for bradycardia. In this experiment, we investigated its therapeutic significance and possible therapeutic targets for sinus bradycardia.
Methods
The bradycardia model of SD rats was prepared by using acetylcholine, propranolol, and verapamil respectively, and treated with Lingbao Huxin Dan for 3 weeks. At the end of treatment, the rats' ECG was recorded using a PV-LOOP heart rate recorder. The hearts and serum samples were collected to detect heart rate and related gene expression which were screened through network pharmacology analysis, as well as to evaluate the effect of Lingbao Huxin Dan on ion channels in the cardiac conduction system.
Results
Lingbao Huxin Dan improved the heart rate in the bradycardia model of SD rats and inhibited AV node conduction block by targeting ion channel proteins in the cardiac conduction system. The underlying molecular mechanism may be the activation of the β-adrenergic receptor-dependent cAMP/PKA signaling pathway targeted to enhance the expression of the pacing channel HCN4. In addition, Lingbao Huxin Dan prevented the deterioration of bradycardia by promoting the production of the oxygen radical scavenger SOD and inhibiting the upregulation of LDH due to cardiomyocyte damage.
Conclusions
Our study proved that Lingbao Huxin Dan alleviated bradycardia by downregulating CX45 and enhancing the expression of HCN4, ADRβ1, and TRPM7 to shorten the atrioventricular node induction period and improve sinoatrial node signaling. In addition, Lingbao Huxin Dan relieved myocardial injury induced by bradycardia by reducing the level of oxygen free radicals in the cardiac microenvironment.
{"title":"Exploring the mechanism of Lingbao Huxin Dan in the treatment of bradycardia based on atrioventricular node electrical signal conduction","authors":"Jing Zhang , Guobin Zheng , Shangjing Liu , Yaodong Miao , Shu Yang , Sheng Li , Zhihui Yang , Danping Zhuo , Rui Guo , Yang Guo , Rongjiang Shao , Yunqing Hua , Chuanrui Ma","doi":"10.1016/j.jhip.2024.12.003","DOIUrl":"10.1016/j.jhip.2024.12.003","url":null,"abstract":"<div><h3>Background</h3><div>Prolonged bradycardia leads to inadequate pumping of the heart, causing myocardial ischemia, which in turn affects cardiac function. Depending on its clinical features, improving the AV node conduction block may be an important tool to inhibit the progression of the disease. Lingbao Huxin Dan, which consists of several traditional Chinese medicines, is used for conditions similar to bradycardia and may be a potential therapeutic agent for bradycardia. In this experiment, we investigated its therapeutic significance and possible therapeutic targets for sinus bradycardia.</div></div><div><h3>Methods</h3><div>The bradycardia model of SD rats was prepared by using acetylcholine, propranolol, and verapamil respectively, and treated with Lingbao Huxin Dan for 3 weeks. At the end of treatment, the rats' ECG was recorded using a PV-LOOP heart rate recorder. The hearts and serum samples were collected to detect heart rate and related gene expression which were screened through network pharmacology analysis, as well as to evaluate the effect of Lingbao Huxin Dan on ion channels in the cardiac conduction system.</div></div><div><h3>Results</h3><div>Lingbao Huxin Dan improved the heart rate in the bradycardia model of SD rats and inhibited AV node conduction block by targeting ion channel proteins in the cardiac conduction system. The underlying molecular mechanism may be the activation of the β-adrenergic receptor-dependent cAMP/PKA signaling pathway targeted to enhance the expression of the pacing channel HCN4. In addition, Lingbao Huxin Dan prevented the deterioration of bradycardia by promoting the production of the oxygen radical scavenger SOD and inhibiting the upregulation of LDH due to cardiomyocyte damage.</div></div><div><h3>Conclusions</h3><div>Our study proved that Lingbao Huxin Dan alleviated bradycardia by downregulating CX45 and enhancing the expression of HCN4, ADRβ1, and TRPM7 to shorten the atrioventricular node induction period and improve sinoatrial node signaling. In addition, Lingbao Huxin Dan relieved myocardial injury induced by bradycardia by reducing the level of oxygen free radicals in the cardiac microenvironment.</div></div>","PeriodicalId":100787,"journal":{"name":"Journal of Holistic Integrative Pharmacy","volume":"5 4","pages":"Pages 333-343"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143174796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-01DOI: 10.1016/j.jhip.2024.11.004
Zepeng Zhang , Ju Liu , Yi Wang , Xiwen Li , Manman Guo , Menglei Ding , Tongtong Zhu , Lei Zhang
Objective
Yiwei Decoction (YWD) is an ancient TCM formula with historical use in treating chronic atrophic gastritis (CAG). However, its pharmacodynamic mechanisms remain poorly understood. This study aims to study the effect of YWD on CAG through network pharmacology and experimental verification.
Methods
UPLC/MS and the SwissADME database were employed to identify active compounds in YWD. YWD targets were screened by SwissTargetPrediction databases, CAG targets were screened by databases, and the two were intersected for PPI network analysis. The target prediction was performed by GO and KEGG analysis. H2O2-induced injury models were established in zebrafish and GES-1 cells, which were subsequently treated with varying doses of YWD. Oxidative stress indicators and apoptosis and inflammation levels in zebrafish and GES-1 cells were assessed using fluorescence microscopy, flow cytometry, and microplate reader assays. The binding capabilities of the core components and core targets were examined using molecular docking. Q-PCR and Western blot were employed to analyze the expression levels of Nrf2, Keap1, and HO-1, respectively.
Results
Forty-nine compounds were identified from YWD. Network pharmacological analysis suggested that YWD may treat CAG by modulating redox-related signaling pathways, inhibiting apoptosis, and reducing inflammation. Subsequent in vitro and in vivo experiments validated these predictions. YWD effectively mitigated H2O2-induced oxidative stress in zebrafish and GES-1 cells, suppressing ROS and decreasing apoptosis. YWD reduced inflammation in gastric epithelial cells. Molecular docking results suggested that methylophiopogonanone A and imperatorin may play a key role in the treatment of YWD. Mechanistically, YWD activated the Nrf2 signaling pathway, downregulated Keap1 expression, and upregulated HO-1 and Bcl2 expression.
Conclusion
YWD could improve oxidative stress indicators, inhibiting cell apoptosis, reducing inflammation, and its molecular mechanism of the CAG treatment may be through the Keap1/Nrf2/HO-1 pathways, and to promote the body's antioxidant system.
{"title":"Network pharmacology-based study on the mechanism of Yiwei Decoction in chronic atrophic gastritis and experimental assessment","authors":"Zepeng Zhang , Ju Liu , Yi Wang , Xiwen Li , Manman Guo , Menglei Ding , Tongtong Zhu , Lei Zhang","doi":"10.1016/j.jhip.2024.11.004","DOIUrl":"10.1016/j.jhip.2024.11.004","url":null,"abstract":"<div><h3>Objective</h3><div>Yiwei Decoction (YWD) is an ancient TCM formula with historical use in treating chronic atrophic gastritis (CAG). However, its pharmacodynamic mechanisms remain poorly understood. This study aims to study the effect of YWD on CAG through network pharmacology and experimental verification.</div></div><div><h3>Methods</h3><div>UPLC/MS and the SwissADME database were employed to identify active compounds in YWD. YWD targets were screened by SwissTargetPrediction databases, CAG targets were screened by databases, and the two were intersected for PPI network analysis. The target prediction was performed by GO and KEGG analysis. H<sub>2</sub>O<sub>2</sub>-induced injury models were established in zebrafish and GES-1 cells, which were subsequently treated with varying doses of YWD. Oxidative stress indicators and apoptosis and inflammation levels in zebrafish and GES-1 cells were assessed using fluorescence microscopy, flow cytometry, and microplate reader assays. The binding capabilities of the core components and core targets were examined using molecular docking. Q-PCR and Western blot were employed to analyze the expression levels of Nrf2, Keap1, and HO-1, respectively.</div></div><div><h3>Results</h3><div>Forty-nine compounds were identified from YWD. Network pharmacological analysis suggested that YWD may treat CAG by modulating redox-related signaling pathways, inhibiting apoptosis, and reducing inflammation. Subsequent <em>in vitro</em> and <em>in vivo</em> experiments validated these predictions. YWD effectively mitigated H<sub>2</sub>O<sub>2</sub>-induced oxidative stress in zebrafish and GES-1 cells, suppressing ROS and decreasing apoptosis. YWD reduced inflammation in gastric epithelial cells. Molecular docking results suggested that methylophiopogonanone A and imperatorin may play a key role in the treatment of YWD. Mechanistically, YWD activated the Nrf2 signaling pathway, downregulated Keap1 expression, and upregulated HO-1 and Bcl2 expression.</div></div><div><h3>Conclusion</h3><div>YWD could improve oxidative stress indicators, inhibiting cell apoptosis, reducing inflammation, and its molecular mechanism of the CAG treatment may be through the Keap1/Nrf2/HO-1 pathways, and to promote the body's antioxidant system.</div></div>","PeriodicalId":100787,"journal":{"name":"Journal of Holistic Integrative Pharmacy","volume":"5 4","pages":"Pages 277-290"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143174792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-05DOI: 10.1016/j.jhip.2024.10.002
Yi Chen , Ying Liu , Jianglian She , Mengjing Cong , Junfeng Wang , Lalith Jayasinghe , Yonghong Liu , Xuefeng Zhou
Objective
To study the chemical compositions from Penicillium sp. SCSIO 41425 and explore their DPPH radical scavenging activity.
Methods
Ethyl acetate extract of Penicillium sp. SCSIO 41425 was separated and purified by silica gel, Ostade-cylsilane (ODS), semi-preparative HPLC, and thin layer chromatography, and their structures were determined by spectroscopic analysis and comparison with the reported literatures.
Results
A total of 18 compounds were isolated from Penicillium sp. SCSIO 41425, including one new compound, (2′R,3′R)-4-(3-hydroxybutan-2-yl)-3,6-dimethylbenzene-1,2-diol (1) and seventeen known compounds (2–18). Their structures were elucidated by detailed NMR and ECD calculations. Compounds 4 and 5 exhibited potent DPPH radical scavenging activity, with EC50 values of 8.42 and 6.62 μg/mL, which were stronger than the positive control ascorbic acid (EC50, 11.22 μg/mL).
Conclusion
This study expands the natural product library of marine cold-seep-derived fungus and provides marine-derived drug source molecules for potent antioxidants.
{"title":"Secondary metabolites from the cold-seep-derived fungus Penicillium sp. SCSIO 41425 and their free radical scavenging activity","authors":"Yi Chen , Ying Liu , Jianglian She , Mengjing Cong , Junfeng Wang , Lalith Jayasinghe , Yonghong Liu , Xuefeng Zhou","doi":"10.1016/j.jhip.2024.10.002","DOIUrl":"10.1016/j.jhip.2024.10.002","url":null,"abstract":"<div><h3>Objective</h3><div>To study the chemical compositions from <em>Penicillium</em> sp. SCSIO 41425 and explore their DPPH radical scavenging activity.</div></div><div><h3>Methods</h3><div>Ethyl acetate extract of <em>Penicillium</em> sp. SCSIO 41425 was separated and purified by silica gel, Ostade-cylsilane (ODS), semi-preparative HPLC, and thin layer chromatography, and their structures were determined by spectroscopic analysis and comparison with the reported literatures.</div></div><div><h3>Results</h3><div>A total of 18 compounds were isolated from <em>Penicillium</em> sp. SCSIO 41425, including one new compound, (2′<em>R</em>,3′<em>R</em>)-4-(3-hydroxybutan-2-yl)-3,6-dimethylbenzene-1,2-diol (<strong>1</strong>) and seventeen known compounds (<strong>2</strong>–<strong>18</strong>). Their structures were elucidated by detailed NMR and ECD calculations. Compounds <strong>4</strong> and <strong>5</strong> exhibited potent DPPH radical scavenging activity, with EC<sub>50</sub> values of 8.42 and 6.62 μg/mL, which were stronger than the positive control ascorbic acid (EC<sub>50</sub>, 11.22 μg/mL).</div></div><div><h3>Conclusion</h3><div>This study expands the natural product library of marine cold-seep-derived fungus and provides marine-derived drug source molecules for potent antioxidants.</div></div>","PeriodicalId":100787,"journal":{"name":"Journal of Holistic Integrative Pharmacy","volume":"5 4","pages":"Pages 257-261"},"PeriodicalIF":0.0,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142586607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-04DOI: 10.1016/j.jhip.2024.10.001
Om Prakash , Amit Kumar , Salil Tiwari, Priyanka Bajpai
Apigenin, a naturally occurring flavonoid found in fruits and vegetables, has received substantial attention due to its various pharmacological effects, particularly in cancer therapy. This review delves deeply into apigenin's phytochemical properties and pharmacological activity, with a particular emphasis on its potential as a targeted cancer therapy. We conducted an exhaustive literature study to investigate the molecular processes underlying apigenin's anticancer actions, such as its ability to induce apoptosis, block angiogenesis, and decrease metastasis. We also examine its synergistic effects with conventional chemotherapy drugs and its potential as an adjuvant therapy. Furthermore, the paper discusses current advances in nanoparticle-based delivery systems that improve the bioavailability and efficacy of apigenin in cancer treatment. This detailed investigation aims to provide insights into apigenin's therapeutic capabilities and future possibilities in targeted cancer treatment.
{"title":"The versatility of apigenin: Especially as a chemopreventive agent for cancer","authors":"Om Prakash , Amit Kumar , Salil Tiwari, Priyanka Bajpai","doi":"10.1016/j.jhip.2024.10.001","DOIUrl":"10.1016/j.jhip.2024.10.001","url":null,"abstract":"<div><div>Apigenin, a naturally occurring flavonoid found in fruits and vegetables, has received substantial attention due to its various pharmacological effects, particularly in cancer therapy. This review delves deeply into apigenin's phytochemical properties and pharmacological activity, with a particular emphasis on its potential as a targeted cancer therapy. We conducted an exhaustive literature study to investigate the molecular processes underlying apigenin's anticancer actions, such as its ability to induce apoptosis, block angiogenesis, and decrease metastasis. We also examine its synergistic effects with conventional chemotherapy drugs and its potential as an adjuvant therapy. Furthermore, the paper discusses current advances in nanoparticle-based delivery systems that improve the bioavailability and efficacy of apigenin in cancer treatment. This detailed investigation aims to provide insights into apigenin's therapeutic capabilities and future possibilities in targeted cancer treatment.</div></div>","PeriodicalId":100787,"journal":{"name":"Journal of Holistic Integrative Pharmacy","volume":"5 4","pages":"Pages 249-256"},"PeriodicalIF":0.0,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142578925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-01DOI: 10.1016/j.jhip.2024.08.003
Lili Huang , Yicong Lei , Yucheng Chen , Xin Hu , Chengyu Huang , Huaqing Lin
As one of the hot materials in biomedical research, alginate gel shows great potential in tumor therapy with its unique physical and chemical properties. In order to better meet the complex needs of cancer treatment, alginate brine gel composite system has come into being. This system not only inherits many advantages of single alginate brine gel, but also significantly improves the mechanical properties of the material through compounding, and effectively overcomes the limitations of application. These composites have been applied to drug delivery carriers, tumor targeting systems and three-dimensional tumor cell models in various forms, showing a wide range of application prospects. This paper aims to review the basic structure and properties of alginate gel and analyze the research progress of alginate gel composite systems in the field of cancer in recent years, to provide a valuable reference for the further expansion of the application of this material in tumor therapy.
{"title":"Research progress in application of alginate gel as tumor drug delivery carrier, for tumor localization and 3D tumor cell model","authors":"Lili Huang , Yicong Lei , Yucheng Chen , Xin Hu , Chengyu Huang , Huaqing Lin","doi":"10.1016/j.jhip.2024.08.003","DOIUrl":"10.1016/j.jhip.2024.08.003","url":null,"abstract":"<div><p>As one of the hot materials in biomedical research, alginate gel shows great potential in tumor therapy with its unique physical and chemical properties. In order to better meet the complex needs of cancer treatment, alginate brine gel composite system has come into being. This system not only inherits many advantages of single alginate brine gel, but also significantly improves the mechanical properties of the material through compounding, and effectively overcomes the limitations of application. These composites have been applied to drug delivery carriers, tumor targeting systems and three-dimensional tumor cell models in various forms, showing a wide range of application prospects. This paper aims to review the basic structure and properties of alginate gel and analyze the research progress of alginate gel composite systems in the field of cancer in recent years, to provide a valuable reference for the further expansion of the application of this material in tumor therapy.</p></div>","PeriodicalId":100787,"journal":{"name":"Journal of Holistic Integrative Pharmacy","volume":"5 3","pages":"Pages 174-184"},"PeriodicalIF":0.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S270736882400044X/pdfft?md5=f29f11a86eb718a899081ba88a8a3df2&pid=1-s2.0-S270736882400044X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142168781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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