Pub Date : 2025-07-04DOI: 10.1016/j.napere.2025.100141
Abdellatif Alami , Yassine Ez zoubi , Mouhcine Fadil , Fatima zahrae Radi , Meriem El Hallabi , Mohammed Ouali Alami , Abdellah Farah
Vector-borne diseases account for approximately 17 % of all infectious diseases, posing a significant threat to global health. Mosquitoes, among the most notorious vectors, transmit deadly pathogens such as West Nile virus, dengue, and malaria, contributing to over 700,000 deaths annually—disproportionately affecting vulnerable populations. The overuse of synthetic pesticides has led to environmental pollution and widespread pest resistance, necessitating the development of eco-friendly alternatives, including plant-derived essential oils (EOs). Artemisia species (Asteraceae family) produce diverse bioactive compounds, such as terpenoids and sesquiterpene lactones, which demonstrate potent larvicidal activity against mosquito vectors like Culex pipiens. This review explores the botanical characteristics, chemical composition, and bioactive properties of Artemisia species, focusing on their EOs as natural bioinsecticides. Special attention is given to the larvicidal efficacy of Artemisia EOs against C. pipiens and recent advancements in encapsulation techniques to enhance their longevity and effectiveness. Given the escalating challenges of insecticide resistance and mosquito-borne diseases, Artemisia EOs represent a sustainable and promising alternative for integrated vector control strategies.
{"title":"Exploring Artemisia essential oils as natural larvicides for Culex mosquito control","authors":"Abdellatif Alami , Yassine Ez zoubi , Mouhcine Fadil , Fatima zahrae Radi , Meriem El Hallabi , Mohammed Ouali Alami , Abdellah Farah","doi":"10.1016/j.napere.2025.100141","DOIUrl":"10.1016/j.napere.2025.100141","url":null,"abstract":"<div><div>Vector-borne diseases account for approximately 17 % of all infectious diseases, posing a significant threat to global health. Mosquitoes, among the most notorious vectors, transmit deadly pathogens such as West Nile virus, dengue, and malaria, contributing to over 700,000 deaths annually—disproportionately affecting vulnerable populations. The overuse of synthetic pesticides has led to environmental pollution and widespread pest resistance, necessitating the development of eco-friendly alternatives, including plant-derived essential oils (EOs). <em>Artemisia</em> species (Asteraceae family) produce diverse bioactive compounds, such as terpenoids and sesquiterpene lactones, which demonstrate potent larvicidal activity against mosquito vectors like <em>Culex pipiens</em>. This review explores the botanical characteristics, chemical composition, and bioactive properties of <em>Artemisia</em> species, focusing on their EOs as natural bioinsecticides. Special attention is given to the larvicidal efficacy of <em>Artemisia</em> EOs against <em>C. pipiens</em> and recent advancements in encapsulation techniques to enhance their longevity and effectiveness. Given the escalating challenges of insecticide resistance and mosquito-borne diseases, <em>Artemisia</em> EOs represent a sustainable and promising alternative for integrated vector control strategies.</div></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"13 ","pages":"Article 100141"},"PeriodicalIF":0.0,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144563268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-06-24DOI: 10.1016/j.napere.2025.100140
Jaime Ruiz-Vega , Carlos Inocencio Cortés-Martínez , Ramón Román-Doval , Manuel Alejandro Valdés-Madrigal , Teodulfo Aquino-Bolaños
In this work, a discrete choice sequential approach for the pelletisation of the entomopathogenic nematode Steinernema glaseri is investigated. First, the pellets were made with agricultural soil (S), sugarcane bagasse ash (A), and diatomaceous earth (DE) in different proportions. Then, the physical stability and moisture retention ability were evaluated to select the best combinations for the pelletisation of infective juveniles (IJs) and the survival and pathogenicity on Galleria mellonella larvae were determined. Second, clay (C) and/or sodium polyacrylate (SP) were added to the best combinations to improve the moisture retention and, the survival and pathogenicity on Tenebrio molitor larvae were evaluated. The initial combinations selected were S85:A15:DE0, S50:A15:DE35 and S80:A15:DE5. The highest mean survival time (16.1 days) of IJs was obtained with S50:C15:DE35 and the pathogenicity in G. mellonella was 20 ± 6.40 %. The S15:C50:A15:DE20:SP1 mixture stood out for its lower moisture loss (90.17 %) when SP (1 g per 100 g of the mixture) and C were added, the survival of S. glaseri increases to 26.4 ± 0.433 days and increased the pathogenicity on T. molitor to 42.5 ± 7.9 %.
{"title":"An experiment-based screening approach to formulate the entomopathogenic nematode Steinernema glaseri in pellets with powder materials","authors":"Jaime Ruiz-Vega , Carlos Inocencio Cortés-Martínez , Ramón Román-Doval , Manuel Alejandro Valdés-Madrigal , Teodulfo Aquino-Bolaños","doi":"10.1016/j.napere.2025.100140","DOIUrl":"10.1016/j.napere.2025.100140","url":null,"abstract":"<div><div>In this work, a discrete choice sequential approach for the pelletisation of the entomopathogenic nematode <em>Steinernema glaseri</em> is investigated. First, the pellets were made with agricultural soil (S), sugarcane bagasse ash (A), and diatomaceous earth (DE) in different proportions. Then, the physical stability and moisture retention ability were evaluated to select the best combinations for the pelletisation of infective juveniles (IJs) and the survival and pathogenicity on <em>Galleria mellonella</em> larvae were determined. Second, clay (C) and/or sodium polyacrylate (SP) were added to the best combinations to improve the moisture retention and, the survival and pathogenicity on <em>Tenebrio molitor</em> larvae were evaluated. The initial combinations selected were S85:A15:DE0, S50:A15:DE35 and S80:A15:DE5. The highest mean survival time (16.1 days) of IJs was obtained with S50:C15:DE35 and the pathogenicity in <em>G. mellonella</em> was 20 ± 6.40 %. The S15:C50:A15:DE20:SP1 mixture stood out for its lower moisture loss (90.17 %) when SP (1 g per 100 g of the mixture) and C were added, the survival of <em>S. glaseri</em> increases to 26.4 ± 0.433 days and increased the pathogenicity on <em>T. molitor</em> to 42.5 ± 7.9 %.</div></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"13 ","pages":"Article 100140"},"PeriodicalIF":0.0,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144517557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-06-17DOI: 10.1016/j.napere.2025.100139
Kandaswamy Kalaivani
The extensive use of synthetic pesticides has led to insect resistance and adverse effects on human health and the environment. As a result, farmers increasingly prefer natural pesticides, which degrade easily without leaving harmful residues in soil and water. Eucalyptus oil, an eco-friendly botanical pesticide, serves as a promising alternative for sustainable pest management. This study evaluates the bio-pesticidal efficacy of commercially available eucalyptus essential oil against Sylepta derogata, a major pest of okra in Tamil Nadu. Laboratory experiments were conducted to assess the impact of eucalyptus oil at concentrations of 25, 50, and 100 ppm on different larval instars. A dose-dependent antifeedant effect was observed across all instars, with significant reductions in larval and pupal weight and length, alongside prolonged larval duration. Deformities in pupae and adults were also recorded. When insects in the 1st and 2nd instars were treated with 100 ppm of eucalyptus oil, a significantly high mortality of nearly 95 % was observed. This result suggests that strong larvicidal efficacy of eucalyptus oil at this concentration. Eucalyptus oil at 10 ppm significantly reduced survivorship, with a marked decline in population growth index compared to the untreated control. These findings suggest that eucalyptus oil exhibits potent pesticidal activity against S. derogata larvae and holds potential as a natural alternative for okra pest management.
{"title":"Efficacy of eucalyptus oil as a sustainable biopesticide for population suppression of Sylepta derogata Fab (Lepidoptera: Pyralidae), an okra pest","authors":"Kandaswamy Kalaivani","doi":"10.1016/j.napere.2025.100139","DOIUrl":"10.1016/j.napere.2025.100139","url":null,"abstract":"<div><div>The extensive use of synthetic pesticides has led to insect resistance and adverse effects on human health and the environment. As a result, farmers increasingly prefer natural pesticides, which degrade easily without leaving harmful residues in soil and water. Eucalyptus oil, an eco-friendly botanical pesticide, serves as a promising alternative for sustainable pest management. This study evaluates the bio-pesticidal efficacy of commercially available eucalyptus essential oil against <em>Sylepta derogata</em>, a major pest of okra in Tamil Nadu. Laboratory experiments were conducted to assess the impact of eucalyptus oil at concentrations of 25, 50, and 100 ppm on different larval instars. A dose-dependent antifeedant effect was observed across all instars, with significant reductions in larval and pupal weight and length, alongside prolonged larval duration. Deformities in pupae and adults were also recorded. When insects in the 1st and 2nd instars were treated with 100 ppm of eucalyptus oil, a significantly high mortality of nearly 95 % was observed. This result suggests that strong larvicidal efficacy of eucalyptus oil at this concentration. Eucalyptus oil at 10 ppm significantly reduced survivorship, with a marked decline in population growth index compared to the untreated control. These findings suggest that eucalyptus oil exhibits potent pesticidal activity against <em>S. derogata</em> larvae and holds potential as a natural alternative for okra pest management.</div></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"13 ","pages":"Article 100139"},"PeriodicalIF":0.0,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144563804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-06-16DOI: 10.1016/j.napere.2025.100138
Ali Nasiri , Sina Fallah , Alessandra Carrubba
The widespread use of chemical herbicides in agroecosystems poses significant environmental risks, driving the need for sustainable alternatives. This study evaluates the inhibitory effects of essential oil (EO) from the stem, leaf, and flower of Echinophora cinerea Boiss. (Apiaceae) on the seed germination of common amaranth (Amaranthus retroflexus), compared with Treflan®, a conventional herbicide. The results showed that concentrations of ≥ 1 µL/mL of E. cinerea EO completely inhibited seed germination, while lower concentrations (0.5 µL/mL) were significantly more effective than the control and Treflan®. Both treatments induced similar levels of malondialdehyde, electrolyte leakage, and hydrogen peroxide in A. retroflexus, with comparable cell viability and proline content (except for leaf EO). Radicle and plumule length in EO-treated plants were reduced by 58–65 % and 16–22 %, respectively, compared to Treflan®’s inhibition of 91 % and 76–85 %. Plumule fresh weight was similar in both EO and Treflan® treatments, though radicle fresh weight was lower with EO (p > 0.05). EO derived from E. cinerea has significant growth inhibitory potential and can induce oxidative stress, making it an effective weed control tool. Overall, essential oil of E. cinerea can be considered as a bioherbicide with an extremely low inhibitory concentration (IC100 ≤ 1 µL/mL). Its effectiveness comparable to Treflan® in reducing weed growth at the same concentration highlights its potential for industrial production as a bioherbicide.
{"title":"A novel herbicide improves functional outcomes in a conventional weed of summer crops: An alternative to Treflan®","authors":"Ali Nasiri , Sina Fallah , Alessandra Carrubba","doi":"10.1016/j.napere.2025.100138","DOIUrl":"10.1016/j.napere.2025.100138","url":null,"abstract":"<div><div>The widespread use of chemical herbicides in agroecosystems poses significant environmental risks, driving the need for sustainable alternatives. This study evaluates the inhibitory effects of essential oil (EO) from the stem, leaf, and flower of <em>Echinophora cinerea</em> Boiss. (Apiaceae) on the seed germination of common amaranth (<em>Amaranthus retroflexus</em>), compared with Treflan®, a conventional herbicide. The results showed that concentrations of ≥ 1 µL/mL of <em>E. cinerea</em> EO completely inhibited seed germination, while lower concentrations (0.5 µL/mL) were significantly more effective than the control and Treflan®. Both treatments induced similar levels of malondialdehyde, electrolyte leakage, and hydrogen peroxide in <em>A. retroflexus</em>, with comparable cell viability and proline content (except for leaf EO). Radicle and plumule length in EO-treated plants were reduced by 58–65 % and 16–22 %, respectively, compared to Treflan®’s inhibition of 91 % and 76–85 %. Plumule fresh weight was similar in both EO and Treflan® treatments, though radicle fresh weight was lower with EO (<em>p</em> > 0.05). EO derived from <em>E. cinerea</em> has significant growth inhibitory potential and can induce oxidative stress, making it an effective weed control tool. Overall, essential oil of <em>E. cinerea</em> can be considered as a bioherbicide with an extremely low inhibitory concentration (IC100 ≤ 1 µL/mL). Its effectiveness comparable to Treflan® in reducing weed growth at the same concentration highlights its potential for industrial production as a bioherbicide.</div></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"13 ","pages":"Article 100138"},"PeriodicalIF":0.0,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144331139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Morocco is widely known for its biodiversity in flora, notably medicinal and aromatic plants (MAP). These plants are widely distributed across the country in different climates, latitudes, and altitudes, and have been reported for various biological activities. This review aims to showcase the insecticidal and behavioral effects of Moroccan essential oils (EO) extracted from different plant families against insect pests. All eligible studies were retrieved from Scopus and Web of Science following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines using ‘Essential oil” and “Insect*” as keywords where 11,400 articles were found and only 58 studies met the aim of this review. The analysis of articles retrieved from Scopus and Web of Science revealed a strong dominance of EOs extracted from the Lamiaceae family, where Mentha pulegium and Rosmarinus officinalis appear as the most studied species. In terms of Insects, the Coleoptera order was the most species evaluated for different bioassays, where Callosobruchus maculatus and Tribolium castaneum were the most studied species. In terms of bioactivities, Moroccan EOs exhibited a potent insecticidal activity via fumigation, contact, ingestion, and topical application. These EOs also affected the behavior of insects, leading to repellent, feeding deterrent, and anti-settling effects. Besides, this review highlights the impact of several factors on reported bioactivities. These factors can be related to EOs such as harvest period, geographical distribution, plant part used, and others related to the insects, such as insect species, developmental stages, and sex of adults. In brief, the diversity of EOs extracted from Moroccan Plants shows a promising capacity for the development of sustainable, natural, and harmless solutions for the control of pests.
摩洛哥以其植物的生物多样性而闻名，特别是药用和芳香植物（MAP）。这些植物广泛分布在全国不同的气候、纬度和海拔，具有多种生物活性。本文综述了从不同植物科中提取的摩洛哥精油对害虫的杀虫和行为作用。所有符合条件的研究都按照系统评价和荟萃分析（PRISMA）指南，以“精油”和“昆虫*”为关键词，从Scopus和Web of Science检索到11,400篇文章，只有58篇研究符合本综述的目的。对Scopus和Web of Science检索的文章进行分析发现，从Lamiaceae科中提取的EOs具有很强的优势，其中Mentha pulegium和Rosmarinus officinalis是研究最多的物种。在昆虫方面，不同生物测定方法评价的种数最多的是鞘翅目，其中斑纹Callosobruchus maculatus和castaneum是研究最多的种。在生物活性方面，摩洛哥EOs通过熏蒸、接触、摄入和局部应用表现出强大的杀虫活性。这些EOs还影响昆虫的行为，导致驱避，摄食威慑和反沉降作用。此外，本文还重点介绍了几种因素对报道的生物活性的影响。这些因素可能与生态环境有关，如采收期、地理分布、所使用的植物部位，也可能与昆虫有关，如昆虫种类、发育阶段和成虫性别。简而言之，从摩洛哥植物中提取的EOs的多样性显示出开发可持续、自然和无害的害虫控制解决方案的潜力。
{"title":"Sustainable pest management with moroccan essential oils: Insights into insecticidal, behavioral effects, and factors influencing bioactivity","authors":"Houssam Annaz , Noureddin Bouayad , Ayoub Kounnoun , Francesco Cacciola , Kacem Rharrabe","doi":"10.1016/j.napere.2025.100137","DOIUrl":"10.1016/j.napere.2025.100137","url":null,"abstract":"<div><div>Morocco is widely known for its biodiversity in flora, notably medicinal and aromatic plants (MAP). These plants are widely distributed across the country in different climates, latitudes, and altitudes, and have been reported for various biological activities. This review aims to showcase the insecticidal and behavioral effects of Moroccan essential oils (EO) extracted from different plant families against insect pests. All eligible studies were retrieved from Scopus and Web of Science following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines using ‘Essential oil” and “Insect*” as keywords where 11,400 articles were found and only 58 studies met the aim of this review. The analysis of articles retrieved from Scopus and Web of Science revealed a strong dominance of EOs extracted from the Lamiaceae family, where <em>Mentha pulegium</em> and <em>Rosmarinus officinalis</em> appear as the most studied species. In terms of Insects, the Coleoptera order was the most species evaluated for different bioassays, where <em>Callosobruchus maculatus</em> and <em>Tribolium castaneum</em> were the most studied species. In terms of bioactivities, Moroccan EOs exhibited a potent insecticidal activity via fumigation, contact, ingestion, and topical application. These EOs also affected the behavior of insects, leading to repellent, feeding deterrent, and anti-settling effects. Besides, this review highlights the impact of several factors on reported bioactivities. These factors can be related to EOs such as harvest period, geographical distribution, plant part used, and others related to the insects, such as insect species, developmental stages, and sex of adults. In brief, the diversity of EOs extracted from Moroccan Plants shows a promising capacity for the development of sustainable, natural, and harmless solutions for the control of pests.</div></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"13 ","pages":"Article 100137"},"PeriodicalIF":0.0,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144194652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-05-26DOI: 10.1016/j.napere.2025.100136
Caio P. Fernandes , Jonatas L. Duarte , Desirane C. Bezerra , Anna E.M.F.M. Oliveira , Rodrigo A.S. Cruz , Lisset Ortiz-Zamora , Wanderlei do Amaral , Cícero Deschamps , Alessandra L. Valverde , Lorane I.S. Hage-Melim , Ricardo M.A. Ferreira , Mateus A. Batista , Raimundo N.P. Souto , Luiz E. da Silva
Ocotea odorifera is also known as sassafras and cinnamon sassafras. It was used as flavoring agent in beverages and other food industrial products, being now used in disinfectants and insecticides. The essential oil, of sassafras, as well as other Brazilian species, has great potential for the food industry, although it is occasionally affected by its volatile composition. New products, such as aqueous nano-emulsions, are considered extremely powerful since they enhance the properties of the oils. Therefore, the aim of this study was to develop this type of colloidal systems with O. odorifera essential oil. The best results were achieved with blends of polysorbate 20/sorbitan monooleate (size diameter <200 nm and polydispersity index <0.1) and no major alterations were observed after the storage. The critical interpretation of the molecular characteristics of the phytochemicals of this essential oil presents an interesting analysis about these essential oil-based nano-emulsions. For Aedes aegypti the estimated LC50 and LC90 values, are respectively, 37.646 and 46.3408 ppm; while for Culex quinquefasciatus the estimated LC50 and LC90 values are 33.3365 and 47.8939 ppm, respectively. The low energy/ecofriendly approach using different non-ionic surfactants by a non-heating method is in accordance with a sustainable concept. Therefore, it opens perspectives for the preparation of sassafras nano-emulsions.
{"title":"Development of nanoemulsions from Oocotea odorifera (Vell.) rohwer through a low energy approach: An ecological larvicide against Aedes aegypti and Culex quinquefasciatus","authors":"Caio P. Fernandes , Jonatas L. Duarte , Desirane C. Bezerra , Anna E.M.F.M. Oliveira , Rodrigo A.S. Cruz , Lisset Ortiz-Zamora , Wanderlei do Amaral , Cícero Deschamps , Alessandra L. Valverde , Lorane I.S. Hage-Melim , Ricardo M.A. Ferreira , Mateus A. Batista , Raimundo N.P. Souto , Luiz E. da Silva","doi":"10.1016/j.napere.2025.100136","DOIUrl":"10.1016/j.napere.2025.100136","url":null,"abstract":"<div><div><em>Ocotea odorifera</em> is also known as sassafras and cinnamon sassafras. It was used as flavoring agent in beverages and other food industrial products, being now used in disinfectants and insecticides. The essential oil, of sassafras, as well as other Brazilian species, has great potential for the food industry, although it is occasionally affected by its volatile composition. New products, such as aqueous nano-emulsions, are considered extremely powerful since they enhance the properties of the oils. Therefore, the aim of this study was to develop this type of colloidal systems with <em>O. odorifera</em> essential oil. The best results were achieved with blends of polysorbate 20/sorbitan monooleate (size diameter <200 nm and polydispersity index <0.1) and no major alterations were observed after the storage. The critical interpretation of the molecular characteristics of the phytochemicals of this essential oil presents an interesting analysis about these essential oil-based nano-emulsions. For <em>Aedes aegypti</em> the estimated LC<sub>50</sub> and LC<sub>90</sub> values, are respectively, 37.646 and 46.3408 ppm; while for <em>Culex quinquefasciatus</em> the estimated LC<sub>50</sub> and LC<sub>90</sub> values are 33.3365 and 47.8939 ppm, respectively. The low energy/ecofriendly approach using different non-ionic surfactants by a non-heating method is in accordance with a sustainable concept. Therefore, it opens perspectives for the preparation of sassafras nano-emulsions.</div></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"13 ","pages":"Article 100136"},"PeriodicalIF":0.0,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144221817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Root-knot nematodes (Meloidogyne spp.) pose a major threat to vegetable production in Morocco. In search of sustainable alternatives to synthetic nematicides, this study evaluated the in vitro nematicidal activity of ethanolic extracts from four Moroccan brown algae: Cystoseira humilis, Cystoseira ericoides, Sargassum vulgare, and Sargassum muticum at concentrations of 6, 8, and 10 mg/mL. The extracts of S. vulgare and C. humilis achieved the highest juvenile mortality (83 %) after 72 hours. S. vulgare also showed the greatest inhibition of egg hatching (82 %). Phytochemical analysis revealed that C. humilis and C. ericoides had the highest levels of total phenolics and flavonoids, while S. vulgare demonstrated strong nematicidal activity despite lower compound concentrations. These findings highlight the potential of S. vulgare and Cystoseira species as eco-friendly agents for managing Meloidogyne spp. Further in vivo studies are required to confirm their efficacy, determine optimal application methods, and establish effective dosages.
{"title":"Moroccan brown algae as a source of bionematicidal against root-knot nematode (Meloidogyne spp.)","authors":"Mouna Belmouden , Khadija Bouftini , Amina Braimi , Bouchra El Aissaoui , Mammass Bourassen , Ibtissam Sabir , Ilyass Filali Alaoui , Tayeb Obidari , Rachid Nmila , El Hassan Mayad , Halima Rchid","doi":"10.1016/j.napere.2025.100135","DOIUrl":"10.1016/j.napere.2025.100135","url":null,"abstract":"<div><div>Root-knot nematodes (<em>Meloidogyne</em> spp.) pose a major threat to vegetable production in Morocco. In search of sustainable alternatives to synthetic nematicides, this study evaluated the <em>in vitro</em> nematicidal activity of ethanolic extracts from four Moroccan brown algae: <em>Cystoseira humilis</em>, <em>Cystoseira ericoides</em>, <em>Sargassum vulgare</em>, and <em>Sargassum muticum</em> at concentrations of 6, 8, and 10 mg/mL. The extracts of <em>S. vulgare</em> and <em>C. humilis</em> achieved the highest juvenile mortality (83 %) after 72 hours. <em>S. vulgare</em> also showed the greatest inhibition of egg hatching (82 %). Phytochemical analysis revealed that <em>C. humilis</em> and <em>C. ericoides</em> had the highest levels of total phenolics and flavonoids, while <em>S. vulgare</em> demonstrated strong nematicidal activity despite lower compound concentrations. These findings highlight the potential of <em>S. vulgare</em> and <em>Cystoseira</em> species as eco-friendly agents for managing <em>Meloidogyne spp.</em> Further <em>in vivo</em> studies are required to confirm their efficacy, determine optimal application methods, and establish effective dosages.</div></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"13 ","pages":"Article 100135"},"PeriodicalIF":0.0,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144135133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-05-16DOI: 10.1016/j.napere.2025.100134
Fatima Zahrae Moussaid , Rachid Lahlali , Said Ezrari , Azeddin El Barnossi , Abdelilah Iraqi Housseini
The development of fungal resistance to fungicides is a significant challenge in agricultural disease management, and the research axis is in current scientific discourse. The objective of the current study aims to search for new biological agents to control Alternaria alternata (A. alternata) causal agent of tomato rot. 73 fungal isolates where tested in the in vitro screening. Aspergillus sp. isolated from decayed tangerine peel microflora, showed the performing antagonistic strain against A. alternata. Aspergillus sp. exhibited significant inhibition of A. alternata; the growth fungal inhibition (%FI) rate was 66.66 ± 0.25 % causing various morphological mycelia changes, swellings, deformations and vacuolization. A. alternata and the antagonist Aspergillus sp. were identified based on morphology trails and molecular tool as A. alternata and Aspergillus nidulans, respectively. Cell-free supernatant (CFS) showed that the metabolites produced by A. nidulans are involved in the control of A. alternata. The half maximal effective concentration (EC50) value of A. alternata was estimated at 1 % (v/v), the inhibition rate was 50.97 ± 0.85 %. Fourier Transform Infra-Red (FT-IR Spectrometry) analysis of organic (ethanol, methanol) and aqueous extracts of CFS revealed distinct characteristic peak values corresponding to various suggested functional groups in the extracts such as phenolic, lipids, and possibly proteins/peptides compounds. In vivo application of A. nidulans strain to tomato fruits significantly reduced postharvest decay caused by A. alternata by 41.34 % after the 10th day of inoculation. Overall, the findings from this study suggest that A. nidulans strain may be a potential promising bio-agent to control the phytopathogen A. alternata.
{"title":"Biological control of Alternaria alternata MW970059, the causal agent of tomato rot, by Aspergillus nidulans MW732187 isolated from green household waste","authors":"Fatima Zahrae Moussaid , Rachid Lahlali , Said Ezrari , Azeddin El Barnossi , Abdelilah Iraqi Housseini","doi":"10.1016/j.napere.2025.100134","DOIUrl":"10.1016/j.napere.2025.100134","url":null,"abstract":"<div><div>The development of fungal resistance to fungicides is a significant challenge in agricultural disease management, and the research axis is in current scientific discourse. The objective of the current study aims to search for new biological agents to control <em>Alternaria alternata</em> (<em>A. alternata</em>) causal agent of tomato rot. 73 fungal isolates where tested in the <em>in vitro</em> screening. <em>Aspergillus</em> sp. isolated from decayed tangerine peel microflora, showed the performing antagonistic strain against <em>A. alternata</em>. <em>Aspergillus</em> sp. exhibited significant inhibition of <em>A. alternata</em>; the growth fungal inhibition (%FI) rate was 66.66 ± 0.25 % causing various morphological mycelia changes, swellings, deformations and vacuolization. <em>A. alternata</em> and the antagonist <em>Aspergillus</em> sp. were identified based on morphology trails and molecular tool as <em>A. alternata</em> and <em>Aspergillus nidulans</em>, respectively. Cell-free supernatant (CFS) showed that the metabolites produced by <em>A. nidulans</em> are involved in the control of <em>A. alternata</em>. The half maximal effective concentration (EC<sub>50</sub>) value of <em>A. alternata</em> was estimated at 1 % (v/v), the inhibition rate was 50.97 ± 0.85 %. Fourier Transform Infra-Red (FT-IR Spectrometry) analysis of organic (ethanol, methanol) and aqueous extracts of CFS revealed distinct characteristic peak values corresponding to various suggested functional groups in the extracts such as phenolic, lipids, and possibly proteins/peptides compounds. <em>In vivo</em> application of <em>A. nidulans</em> strain to tomato fruits significantly reduced postharvest decay caused by <em>A. alternata</em> by 41.34 % after the 10<sup>th</sup> day of inoculation. Overall, the findings from this study suggest that <em>A. nidulans</em> strain may be a potential promising bio-agent to control the phytopathogen <em>A. alternata</em>.</div></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"13 ","pages":"Article 100134"},"PeriodicalIF":0.0,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144116813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-05-15DOI: 10.1016/j.napere.2025.100133
Camila Renson , Modester D. Nkungu , Regina P. Mtei
The invasive pest Tuta absoluta (Meyrick) poses a significant risk to global tomato crops, particularly affecting Tanzania's food security and economy. Currently, control strategies for this pest rely on synthetic pesticides, which are ineffective and pose a great environmental threat to the ecosystem. This underscores the urgent need for search of ecofriendly alternative to combat this invasive pest. The agar plate method was employed for isolation and purification of fungi from the soil. From soil samples collected in Arusha's tomato fields, 36 fungal isolates were discovered, and subjected to rigorous morphological and biochemical characterization. Of these, 8 demonstrating enzyme activity capable of breaking down insect cuticles, indicating their potential as insecticides. These isolates were subjected molecular characterizations, including DNA sequencing and analyses using the BLAST program available at the National Center for Biological Information (NCBI), to ascertain their identity. The isolates showed 100 % similarity with Aspergillus terreus, Penicillium steckii, Purpureocillium lilacinum, Aspergillus flavus and Aspergillus versicolor of GeneBank accession numbers MN463005.1, MK179265.1, MN242828.1, MN559660.1 and MH270605.1, respectively. Bioassays conducted on T. absoluta larvae with spore suspensions of these fungi revealed significant larval mortality rates for A. terreus, P. steckii, and two strains of P. lilacinum, with Penicillium steckii achieving up to 100 % mortality at a concentration of 107 spores/mL. The study findings highlight the potential of using native fungi, particularly Aspergillus terreus, Penicillium steckii, and Purpureocillium lilacinum, as effective biopesticides against T. absoluta, underscoring the importance of harnessing indigenous microbial resources for sustainable agricultural pest control and food security.
{"title":"Harnessing indigenous entomopathogenic fungi for biopesticide development against Tuta absoluta in Tanzania","authors":"Camila Renson , Modester D. Nkungu , Regina P. Mtei","doi":"10.1016/j.napere.2025.100133","DOIUrl":"10.1016/j.napere.2025.100133","url":null,"abstract":"<div><div>The invasive pest <em>Tuta absoluta</em> (Meyrick) poses a significant risk to global tomato crops, particularly affecting Tanzania's food security and economy. Currently, control strategies for this pest rely on synthetic pesticides, which are ineffective and pose a great environmental threat to the ecosystem. This underscores the urgent need for search of ecofriendly alternative to combat this invasive pest. The agar plate method was employed for isolation and purification of fungi from the soil. From soil samples collected in Arusha's tomato fields, 36 fungal isolates were discovered, and subjected to rigorous morphological and biochemical characterization. Of these, 8 demonstrating enzyme activity capable of breaking down insect cuticles, indicating their potential as insecticides. These isolates were subjected molecular characterizations, including DNA sequencing and analyses using the BLAST program available at the National Center for Biological Information (NCBI), to ascertain their identity. The isolates showed 100 % similarity with <em>Aspergillus terreus</em>, <em>Penicillium steckii</em>, <em>Purpureocillium lilacinum, Aspergillus flavus and Aspergillus versicolor</em> of GeneBank accession numbers MN463005.1, MK179265.1, MN242828.1, MN559660.1 and MH270605.1, respectively. Bioassays conducted on <em>T. absoluta</em> larvae with spore suspensions of these fungi revealed significant larval mortality rates for <em>A. terreus</em>, <em>P. steckii</em>, and two strains of <em>P. lilacinum</em>, with <em>Penicillium steckii</em> achieving up to 100 % mortality at a concentration of 10<sup>7</sup> spores/mL. The study findings highlight the potential of using native fungi, particularly <em>Aspergillus terreus</em>, <em>Penicillium steckii</em>, and <em>Purpureocillium lilacinum</em>, as effective biopesticides against <em>T. absoluta</em>, underscoring the importance of harnessing indigenous microbial resources for sustainable agricultural pest control and food security.</div></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"13 ","pages":"Article 100133"},"PeriodicalIF":0.0,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144105288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Microbial control of forest insects has emerged as a viable alternative to conventional chemical pesticides, providing sustainable strategies for managing forest pest populations. This bibliometric analysis examines the progression of research in this domain from 1964 to 2024, emphasizing the application of entomopathogenic fungi, bacteria, viruses, and nematodes as biological control agents. VOSviewer, Bibliometrix, and Biblioshiny from R were employed for statistical analysis of the articles retrieved from the Scopus database. The research indicates a substantial rise in articles concerning microbial control during the early 2000s, underscoring an increasing awareness of its potential in forest management. Prominent biocontrol agents, including Beauveria bassiana, Metarhizium anisopliae, and Bacillus thuringiensis, have been recognized for their efficacy in managing diverse forest pests, such as the gypsy moth (Lymantria dispar), bark beetles, and defoliators. The research highlights the increasing significance of microbial agents in minimizing pest outbreaks intensified by climate change, invading species, and the constraints of chemical pesticides. Notwithstanding the optimistic outlook, the study also delineates additional challenges, such as ecological hazards, variable efficacy across different climatic conditions, regulatory obstacles, and the absence of long-term field data. These problems underscore the necessity for more studies to enhance the use of microbial management in forest ecosystems, ensuring its efficacy and safety. This research offers a thorough examination of the present condition of microbial control in forest pest management, highlighting the necessity of creating dependable, environmentally sustainable alternatives to maintain forest health and biodiversity amid increasing environmental challenges.
{"title":"Microbial control of forest insect pests over 60 years (1964–2024): Network analysis and bibliometric mapping","authors":"Deepak Kumar Mahanta , Charishma Krishnappa , Tanmaya Kumar Bhoi","doi":"10.1016/j.napere.2025.100132","DOIUrl":"10.1016/j.napere.2025.100132","url":null,"abstract":"<div><div>Microbial control of forest insects has emerged as a viable alternative to conventional chemical pesticides, providing sustainable strategies for managing forest pest populations. This bibliometric analysis examines the progression of research in this domain from 1964 to 2024, emphasizing the application of entomopathogenic fungi, bacteria, viruses, and nematodes as biological control agents. VOSviewer, Bibliometrix, and Biblioshiny from R were employed for statistical analysis of the articles retrieved from the Scopus database. The research indicates a substantial rise in articles concerning microbial control during the early 2000s, underscoring an increasing awareness of its potential in forest management. Prominent biocontrol agents, including <em>Beauveria bassiana</em>, <em>Metarhizium anisopliae</em>, and <em>Bacillus thuringiensis</em>, have been recognized for their efficacy in managing diverse forest pests, such as the gypsy moth (<em>Lymantria dispar</em>), bark beetles, and defoliators. The research highlights the increasing significance of microbial agents in minimizing pest outbreaks intensified by climate change, invading species, and the constraints of chemical pesticides. Notwithstanding the optimistic outlook, the study also delineates additional challenges, such as ecological hazards, variable efficacy across different climatic conditions, regulatory obstacles, and the absence of long-term field data. These problems underscore the necessity for more studies to enhance the use of microbial management in forest ecosystems, ensuring its efficacy and safety. This research offers a thorough examination of the present condition of microbial control in forest pest management, highlighting the necessity of creating dependable, environmentally sustainable alternatives to maintain forest health and biodiversity amid increasing environmental challenges.</div></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"12 ","pages":"Article 100132"},"PeriodicalIF":0.0,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143887020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
扫码关注我们
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号