Pub Date : 2024-11-26DOI: 10.1016/j.napere.2024.100106
Hassan A. Gad , Mohamed S. Al-Anany , Ayman A.M. Atta , Adel A. Al-Ayat , Samir A.M. Abdelgaleil
The toxicity of two microbial insecticides, abamectin (AB) and emamectin benzoate (EM) at 0.25 and 0.5 mg/kg, and three monoterpenes, carvone (CA), p-cymene (CY), and menthone (ME) at 0.625, 1.25, and 2.5 g/kg and their combined treatments was assessed against Callosobruchus maculatus (F.) on cowpea seeds. Adult mortality (after 3 and 7 days), progeny production (after 45 days), and weight loss in cowpea seeds were checked for all individual and combined treatments. Seven days post-exposure, the highest concentrations of most insecticides/monoterpenes combinations showed a total adult mortality (100 %). Similarly, no progeny was observed at the highest rates of combined treatments, especially when EM mixed with either CA or ME. In addition, all combinations of tested monoterpenes and microbial insecticides decreased or suppressed the damage caused by cowpea beetle on seeds and gave a complete protection for the cowpea seeds for 45 days. These findings indicate that combinations of microbial insecticides with monoterpenes could be a useful approach for the effective control of C. maculatus on stored cowpea seeds.
评估了两种微生物杀虫剂（阿维菌素（AB）和苯甲酸阿维菌素（EM），0.25 和 0.5 mg/kg）和三种单萜烯类化合物（香芹酮（CA）、对伞花烯（CY）和薄荷酮（ME），0.625、1.25 和 2.5 g/kg）及其组合处理对豇豆种子上的大斑胼胝体（Callosobruchus maculatus (F.)）的毒性。检查了所有单独处理和混合处理的成虫死亡率（3 天和 7 天后）、后代产量（45 天后）以及豇豆种子的重量损失。接触后 7 天，大多数杀虫剂/单萜组合的最高浓度都会导致成虫完全死亡（100%）。同样，在最高浓度的组合处理中，尤其是当 EM 与 CA 或 ME 混合使用时，没有观察到后代。此外，所有受测单萜与微生物杀虫剂的组合都能减少或抑制豇豆甲虫对种子造成的损害，并对豇豆种子提供长达 45 天的完全保护。这些研究结果表明，微生物杀虫剂与单萜烯类化合物的组合可能是有效控制豇豆种子上大斑金龟子的有效方法。
{"title":"Efficacy of abamectin and emamectin benzoate alone or in binary combinations with carvone, p-cymene and menthone for the control of Callosobruchus maculatus","authors":"Hassan A. Gad , Mohamed S. Al-Anany , Ayman A.M. Atta , Adel A. Al-Ayat , Samir A.M. Abdelgaleil","doi":"10.1016/j.napere.2024.100106","DOIUrl":"10.1016/j.napere.2024.100106","url":null,"abstract":"<div><div>The toxicity of two microbial insecticides, abamectin (AB) and emamectin benzoate (EM) at 0.25 and 0.5 mg/kg, and three monoterpenes, carvone (CA), p-cymene (CY), and menthone (ME) at 0.625, 1.25, and 2.5 g/kg and their combined treatments was assessed against <em>Callosobruchus maculatus</em> (F.) on cowpea seeds. Adult mortality (after 3 and 7 days), progeny production (after 45 days), and weight loss in cowpea seeds were checked for all individual and combined treatments. Seven days post-exposure, the highest concentrations of most insecticides/monoterpenes combinations showed a total adult mortality (100 %). Similarly, no progeny was observed at the highest rates of combined treatments, especially when EM mixed with either CA or ME. In addition, all combinations of tested monoterpenes and microbial insecticides decreased or suppressed the damage caused by cowpea beetle on seeds and gave a complete protection for the cowpea seeds for 45 days. These findings indicate that combinations of microbial insecticides with monoterpenes could be a useful approach for the effective control of <em>C. maculatus</em> on stored cowpea seeds.</div></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"11 ","pages":"Article 100106"},"PeriodicalIF":0.0,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142722800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fungal phytopathogens are the primary causative agents of plant diseases and can significantly limit crop production. As an alternative to synthetic fungicides, often associated with detrimental environmental effects, the use of botanicals is a promising tool for managing fungal infections. This study analysed the phytochemical profiles, as well as the antioxidant and antifungal effect of Arctotis arctotoides (L.F.) Hoffm. The phytochemical profiles were determined using spectrophotometry and Liquid Chromatography-Mass Spectrometry (LC-MS), while two chemical test models were used to assess the antioxidant effect of the plant extract. The antifungal activity was evaluated against Pythium ultimum using the agar well diffusion method. Varying concentrations of total phenolics (131.70 mg gallic acid equivalents per gram (GAE/g) of dry weight (DW) for acetone and 231.56 mg GAE/g DW for methanol extracts) and flavonoids (11.36 mg quercetin equivalents per gram (QE/g) of dry weight (DW) for acetone and 9.86 mg QE/g DW for methanol extracts) were recorded. The LC-MS analysis of the plant extract revealed 14 tentatively identified compounds and 7 unknown bioactive compounds. Two of the compounds, dehydrocostus lactone and methyl pheophorbide A, have been associated with antioxidant effects. Acetone extract (16.67 mm) and methanol extract (18.33 mm) of Arctotis arctotoides exhibited considerable inhibitory effects against P. ultimum. Further antifungal assessment of the identified bioactive compounds remains essential to establish their activity against phytopathogens relevant in the agricultural sector, especially under field conditions.
{"title":"Phytochemical characterization and biological properties of Arctotis arctotoides (L.f.) O. Hoffm related to the management of Pythium root rot disease","authors":"James Lwambi Mwinga , Peter Tshepiso Ndhlovu , Bongisiwe Gladys Shelembe , Olaniyi Amos Fawole , Adeyemi Oladapo Aremu , Wilfred Otang-Mbeng","doi":"10.1016/j.napere.2024.100105","DOIUrl":"10.1016/j.napere.2024.100105","url":null,"abstract":"<div><div>Fungal phytopathogens are the primary causative agents of plant diseases and can significantly limit crop production. As an alternative to synthetic fungicides, often associated with detrimental environmental effects, the use of botanicals is a promising tool for managing fungal infections. This study analysed the phytochemical profiles, as well as the antioxidant and antifungal effect of <em>Arctotis arctotoides</em> (L.F.) Hoffm. The phytochemical profiles were determined using spectrophotometry and Liquid Chromatography-Mass Spectrometry (LC-MS), while two chemical test models were used to assess the antioxidant effect of the plant extract. The antifungal activity was evaluated against <em>Pythium ultimum</em> using the agar well diffusion method. Varying concentrations of total phenolics (131.70 mg gallic acid equivalents per gram (GAE/g) of dry weight (DW) for acetone and 231.56 mg GAE/g DW for methanol extracts) and flavonoids (11.36 mg quercetin equivalents per gram (QE/g) of dry weight (DW) for acetone and 9.86 mg QE/g DW for methanol extracts) were recorded. The LC-MS analysis of the plant extract revealed 14 tentatively identified compounds and 7 unknown bioactive compounds. Two of the compounds, dehydrocostus lactone and methyl pheophorbide A, have been associated with antioxidant effects. Acetone extract (16.67 mm) and methanol extract (18.33 mm) of <em>Arctotis arctotoides</em> exhibited considerable inhibitory effects against <em>P</em>. <em>ultimum</em>. Further antifungal assessment of the identified bioactive compounds remains essential to establish their activity against phytopathogens relevant in the agricultural sector, especially under field conditions.</div></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"11 ","pages":"Article 100105"},"PeriodicalIF":0.0,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142744718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tylenchulus semipenetrans is one of the most destructive citrus pest globally, causing significant crop losses. Its management is challenging due to its broad host range and adaptability to diverse conditions. While nematicides are commonly used, they increase production costs and pose risks to health and the environment. This study aims to develop an alternative control method for this plant parasite by investigating the potential of two arbuscular mycorrhizal fungi (AMF), Rhizophagus irregularis (C. Walker and A. Schüßler, no. BGCBJ09 and Funneliformis mosseae (Gerd. and Trappe, BEG no. 12), under greenhouse conditions. Results demonstrated that both AMF strains significantly reduced T. semipenetrans populations in the soil and roots of citrus rootstocks. In soil, F. mosseae and R. irregularis reduced nematode populations by 95.44 % and 86.5 % in Citrus aurantium, and by 95.57 % and 80.47 % in Carrizo citrange, respectively. In roots, reductions were 81.55 % and 63.10 % for C. aurantium with R. irregularis and F. mosseae, respectively, and 88.89 % and 77.78 % for C. citrange with F. mosseae and R. irregularis, respectively. Additionally, AMF significantly enhanced the growth and biomass of citrus rootstocks compared to the control group infected solely with T. semipenetrans.
半知菌（Tylenchulus semipenetrans）是全球最具破坏性的柑橘害虫之一，会给作物造成重大损失。由于其寄主范围广，对各种条件的适应性强，因此其管理具有挑战性。虽然杀线虫剂很常用，但它们会增加生产成本，并对健康和环境构成风险。本研究旨在通过研究两种节肢型菌根真菌（AMF）--Rhizophagus irregularis（C. Walker and A. Schüßler，No.BGCBJ09 和 Funneliformis mosseae (Gerd. and Trappe, BEG no. 12)）在温室条件下的潜力。结果表明，这两种 AMF 菌株都能显著减少土壤和柑橘砧木根部的半知菌数量。在土壤中，F. mosseae 和 R. irregularis 分别减少了枳实中 95.44 % 和 86.5 % 的线虫数量，以及 Carrizo citrange 中 95.57 % 和 80.47 % 的线虫数量。在根部，枳壳与 R. mosseae 和 F. mosseae 分别减少了 81.55 % 和 63.10 %，柑橘与 F. mosseae 和 R. mosseae 分别减少了 88.89 % 和 77.78 %。此外，与只感染半知菌的对照组相比，AMF 能显著提高柑橘砧木的生长和生物量。
{"title":"Effectiveness of the arbuscular mycorrhizal fungi Funneliformis mossae and Rhizophagus irregularis as biological control agent of the citrus nematode Tylenchulus semipenetrans","authors":"Btissam Zoubi , Fouad Mokrini , Siham Houssayni , Abderrazak Benkebboura , Oumaima Akachoud , Cherki Ghoulam , Abdelilah Iraqi Housseini , Ahmed Qaddoury","doi":"10.1016/j.napere.2024.100104","DOIUrl":"10.1016/j.napere.2024.100104","url":null,"abstract":"<div><div><em>Tylenchulus semipenetrans</em> is one of the most destructive citrus pest globally, causing significant crop losses. Its management is challenging due to its broad host range and adaptability to diverse conditions. While nematicides are commonly used, they increase production costs and pose risks to health and the environment. This study aims to develop an alternative control method for this plant parasite by investigating the potential of two arbuscular mycorrhizal fungi (AMF), <em>Rhizophagus irregularis</em> (C. Walker and A. Schüßler, no. BGCBJ09 and <em>Funneliformis mosseae</em> (Gerd. and Trappe, BEG no. 12), under greenhouse conditions. Results demonstrated that both AMF strains significantly reduced <em>T. semipenetrans</em> populations in the soil and roots of citrus rootstocks. In soil, <em>F. mosseae</em> and <em>R. irregularis</em> reduced nematode populations by 95.44 % and 86.5 % in <em>Citrus aurantium</em>, and by 95.57 % and 80.47 % in <em>Carrizo citrange</em>, respectively. In roots, reductions were 81.55 % and 63.10 % for <em>C. aurantium</em> with <em>R. irregularis</em> and <em>F. mosseae</em>, respectively, and 88.89 % and 77.78 % for <em>C. citrange</em> with <em>F. mosseae</em> and <em>R. irregularis</em>, respectively. Additionally, AMF significantly enhanced the growth and biomass of citrus rootstocks compared to the control group infected solely with <em>T. semipenetrans</em>.</div></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"11 ","pages":"Article 100104"},"PeriodicalIF":0.0,"publicationDate":"2024-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142722808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Secondary metabolic compounds were investigated on the various insecticides from plants based on extraction and profile identified on various toxic substances. Generally, interactions between insects and plants lead to the release of various biochemical components pivotal in secondary metabolic processes and in insect defense against stimuli or insecticides. This study highlighted the efficacy of botanical pesticides containing bioactive chemicals as insecticides, operating through mechanisms such as antifeedants, repellents, protectants, and growth-disrupting hormones. Thus, secondary metabolic activity was confirmed to exhibit insecticidal properties, including the emission of signalling cues such as 2,4-Decadienal, Pterin-6-carboxylic acid, Oleic Acid, 9-Octadecenoic acid (E), and Stearic acid. Meanwhile, Principal component analysis was used to assessed the distribution of metabolites resulting from plant-insect interactions. Additionally, putative toxic substances were confirmed in repellent assays, affirming that botanical blends enhanced certain plant defenses against banana pests. Further understanding of these components and their varying efficacy levels under different conditions may be crucial in developing bio-rational control against B. subcostata under in vitro conditions. The current study aims to examine certain plant extracts as natural enemies and alternatives against the banana fruit scarring beetle B. subcostata under in vitro conditions.
{"title":"Efficacy of botanical pesticides in insecticidal activity against the banana fruit scarring beetle Basilepta subcostata an In vitro analysis","authors":"Velavan Viswakethu , Vinitha Ramasamy , Padmanaban Balakrishnan , Baskar Narayanasamy , Raju Karthic","doi":"10.1016/j.napere.2024.100101","DOIUrl":"10.1016/j.napere.2024.100101","url":null,"abstract":"<div><div>Secondary metabolic compounds were investigated on the various insecticides from plants based on extraction and profile identified on various toxic substances. Generally, interactions between insects and plants lead to the release of various biochemical components pivotal in secondary metabolic processes and in insect defense against stimuli or insecticides. This study highlighted the efficacy of botanical pesticides containing bioactive chemicals as insecticides, operating through mechanisms such as antifeedants, repellents, protectants, and growth-disrupting hormones. Thus, secondary metabolic activity was confirmed to exhibit insecticidal properties, including the emission of signalling cues such as 2,4-Decadienal, Pterin-6-carboxylic acid, Oleic Acid, 9-Octadecenoic acid (E), and Stearic acid. Meanwhile, Principal component analysis was used to assessed the distribution of metabolites resulting from plant-insect interactions. Additionally, putative toxic substances were confirmed in repellent assays, affirming that botanical blends enhanced certain plant defenses against banana pests. Further understanding of these components and their varying efficacy levels under different conditions may be crucial in developing bio-rational control against <em>B. subcostata</em> under <em>in vitro</em> conditions. The current study aims to examine certain plant extracts as natural enemies and alternatives against the banana fruit scarring beetle <em>B. subcostata</em> under <em>in vitro</em> conditions.</div></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"11 ","pages":"Article 100101"},"PeriodicalIF":0.0,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142744716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-17DOI: 10.1016/j.napere.2024.100099
Quang Le Dang , Cuong Quoc Nguyen , Thi Kieu Anh Vo , Thi Thu Trang Nguyen , Quang Duong Pham , Thi Xuyen Nguyen , Thi Hong Cao , Quang De Tran , The Tam Le , Thi Hanh Do , Van Tan Chu , Thi Bich Hong Nguyen
A botanical nanoemulsion (NE) comprised of cinnamaldehyde (from Cinnamomum cassia oil) and annonaceous acetogenin (AAs) (from Annona squamocin seeds extract) was fabricated using sonication. The nanoemulsion was tested for their in vitro and in vivo antifungal activity against Colletotrichum sp. and Fusarium oxysporum the disease-causal agents in fruit and vegetable crops. The best average of nanoemulsion hydrodynamic diameter was 245.3 nm, and the polydispersity index (PI) was 0.499 based on DLS measurement. Zeta potential of the nanoemulsion was determined to be −44.2 mV, indicating the stability of the droplets. In vitro antifungal bioassay of the nanoemulsion showed the best inhibition against Colletotrichum sp. by 53.9 % when tested at a dilution rate of 1:100 (v/v). For F. oxysporum, the nanomaterial also inhibited mycelial growth by 91.6 % at the same dilution rate. When tested at dilution rates of 1:200 and 1:400 (v/v), the nanoemulsions caused moderate and weak inhibitions from 30.9 % to 52.8 % for all fungi. In vivo experiment, the nano-emulsion at 1:200 (v/v) dilution demonstrated an antifungal efficacy of 57.1 % (after 4 days) and 49.6 % (after 7 days) of testing. Besides, the mechanism of action has also been elucidated through molecular interaction models between the main compounds with eburicol 14α-demethylase. Our research indicates that botanical nanoemulsion containing cinnamon oil and AAs have a remarkable potential to suppress phytopathogenic fungi and prevent the spread of fungal diseases in tropical fruits and vegetables such as tomato and other crops of Solanaceae family.
{"title":"A botanical nanoemulsion against phytopathogenic fungi Colletotrichum sp. and Fusarium oxysporum: Preparation, in vitro and in vivo bioassay","authors":"Quang Le Dang , Cuong Quoc Nguyen , Thi Kieu Anh Vo , Thi Thu Trang Nguyen , Quang Duong Pham , Thi Xuyen Nguyen , Thi Hong Cao , Quang De Tran , The Tam Le , Thi Hanh Do , Van Tan Chu , Thi Bich Hong Nguyen","doi":"10.1016/j.napere.2024.100099","DOIUrl":"10.1016/j.napere.2024.100099","url":null,"abstract":"<div><div>A botanical nanoemulsion (NE) comprised of cinnamaldehyde (from Cinnamomum cassia oil) and annonaceous acetogenin (AAs) (from Annona squamocin seeds extract) was fabricated using sonication. The nanoemulsion was tested for their in vitro and in vivo antifungal activity against Colletotrichum sp. and Fusarium oxysporum the disease-causal agents in fruit and vegetable crops. The best average of nanoemulsion hydrodynamic diameter was 245.3 nm, and the polydispersity index (PI) was 0.499 based on DLS measurement. Zeta potential of the nanoemulsion was determined to be −44.2 mV, indicating the stability of the droplets. In vitro antifungal bioassay of the nanoemulsion showed the best inhibition against Colletotrichum sp. by 53.9 % when tested at a dilution rate of 1:100 (v/v). For F. oxysporum, the nanomaterial also inhibited mycelial growth by 91.6 % at the same dilution rate. When tested at dilution rates of 1:200 and 1:400 (v/v), the nanoemulsions caused moderate and weak inhibitions from 30.9 % to 52.8 % for all fungi. In vivo experiment, the nano-emulsion at 1:200 (v/v) dilution demonstrated an antifungal efficacy of 57.1 % (after 4 days) and 49.6 % (after 7 days) of testing. Besides, the mechanism of action has also been elucidated through molecular interaction models between the main compounds with eburicol 14α-demethylase. Our research indicates that botanical nanoemulsion containing cinnamon oil and AAs have a remarkable potential to suppress phytopathogenic fungi and prevent the spread of fungal diseases in tropical fruits and vegetables such as tomato and other crops of Solanaceae family.</div></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"10 ","pages":"Article 100099"},"PeriodicalIF":0.0,"publicationDate":"2024-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142700678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-13DOI: 10.1016/j.napere.2024.100100
Satya Singh , Parthkumar P. Dave , Neeraj Kumar Sethiya , Hardev Choudhary
Earias vittella (Fabricius, 1794) poses a significant threat to Okra production across Asia, Oceania, Africa, Europe, and the Middle East, potentially leading to over 60 % crop losses. Okra, a versatile crop with applications in various industries including pharmaceuticals and food, faces economic peril due to E. vittella infestation, impacting food security. Chemical control methods, while effective, have environmental and health impacts alongside fostering resistance and pest resurgence. Consequently, exploring plant-based alternatives for sustainable pest management has gained traction. This bibliometric study reviewed over 5780 reports published during 1980–2023 and reports 104 research articles selected as per predefined criteria of using plant based E. vittella management in Okra. A total of 108 plant species and their active phytoconstituents belonging to 39 botanical families have been used in different forms comprising of extracts, powders, essential oils, dusts and nanoformulations for E. vittella management in Okra. Their efficacy, impacts on various life parameters of pest, mechanisms of action and their implications in botanical formulation development are also discussed. The review also addresses bibliometric indicators, and the challenges hindering botanical formulations' development faced by various stakeholders. By offering a comprehensive understanding of plant-derived interventions' research landscape, this study aims to address knowledge gaps and provide essential insights for the development of cost-effective, eco-friendly plant-based pest management technologies, crucial for safeguarding Okra production, ultimately contributing to sustainable agriculture.
{"title":"Plant-derivatives for the management of Earias vittella (Fabricius) (Nolidae: Lepidoptera): Current status, challenges, and future prospects","authors":"Satya Singh , Parthkumar P. Dave , Neeraj Kumar Sethiya , Hardev Choudhary","doi":"10.1016/j.napere.2024.100100","DOIUrl":"10.1016/j.napere.2024.100100","url":null,"abstract":"<div><div><em>Earias vittella</em> (Fabricius, 1794) poses a significant threat to Okra production across Asia, Oceania, Africa, Europe, and the Middle East, potentially leading to over 60 % crop losses. Okra, a versatile crop with applications in various industries including pharmaceuticals and food, faces economic peril due to <em>E. vittella</em> infestation, impacting food security. Chemical control methods, while effective, have environmental and health impacts alongside fostering resistance and pest resurgence. Consequently, exploring plant-based alternatives for sustainable pest management has gained traction. This bibliometric study reviewed over 5780 reports published during 1980–2023 and reports 104 research articles selected as per predefined criteria of using plant based <em>E. vittella</em> management in Okra. A total of 108 plant species and their active phytoconstituents belonging to 39 botanical families have been used in different forms comprising of extracts, powders, essential oils, dusts and nanoformulations for <em>E. vittella</em> management in Okra. Their efficacy, impacts on various life parameters of pest, mechanisms of action and their implications in botanical formulation development are also discussed. The review also addresses bibliometric indicators, and the challenges hindering botanical formulations' development faced by various stakeholders. By offering a comprehensive understanding of plant-derived interventions' research landscape, this study aims to address knowledge gaps and provide essential insights for the development of cost-effective, eco-friendly plant-based pest management technologies, crucial for safeguarding Okra production, ultimately contributing to sustainable agriculture.</div></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"10 ","pages":"Article 100100"},"PeriodicalIF":0.0,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142700679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-12DOI: 10.1016/j.napere.2024.100098
Muhammad Salman Hameed , Abdul Basit , Muhammad Humza , Nida Urooj , Muhammad Qasim , Muhammad Anwar
Cabbage (Brassica oleracea) is a globally important crop but often suffers from damage caused by sucking insect pests and various diseases. This study explores the effectiveness of microbial protein elicitors, such as PeaT1, PeBL1, Hrip1, and PeBA1, in managing Cabbage aphids (Brevicoryne brassicae). It investigates how these elicitors affect phytohormone pathways, including jasmonic acid, salicylic acid, and ethylene, and examines their impact on leaf structures, particularly trichomes. The research offers a novel approach by thoroughly assessing the mechanisms through which microbial protein elicitors control cabbage aphids. It provides valuable insights into potential new strategies for pest management in agriculture. By clarifying how elicitors interact with aphids, this study contributes to developing sustainable pest control and crop protection methods.
{"title":"Evaluating microbial protein elicitors for managing Cabbage Aphids in Brassica oleracea","authors":"Muhammad Salman Hameed , Abdul Basit , Muhammad Humza , Nida Urooj , Muhammad Qasim , Muhammad Anwar","doi":"10.1016/j.napere.2024.100098","DOIUrl":"10.1016/j.napere.2024.100098","url":null,"abstract":"<div><div>Cabbage (<em>Brassica oleracea</em>) is a globally important crop but often suffers from damage caused by sucking insect pests and various diseases. This study explores the effectiveness of microbial protein elicitors, such as PeaT1, PeBL1, Hrip1, and PeBA1, in managing Cabbage aphids (<em>Brevicoryne brassicae</em>). It investigates how these elicitors affect phytohormone pathways, including jasmonic acid, salicylic acid, and ethylene, and examines their impact on leaf structures, particularly trichomes. The research offers a novel approach by thoroughly assessing the mechanisms through which microbial protein elicitors control cabbage aphids. It provides valuable insights into potential new strategies for pest management in agriculture. By clarifying how elicitors interact with aphids, this study contributes to developing sustainable pest control and crop protection methods.</div></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"10 ","pages":"Article 100098"},"PeriodicalIF":0.0,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142663846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-10DOI: 10.1016/j.napere.2024.100103
Esmat A. El-Solimany , Antar A. Abdelhamid , Marwa Adel Thabet , Mohamed A. Gad
Using synthetic pesticides is the main strategy for controlling pests. However, these compounds have caused worry because of their harmful effects on health and their diminishing efficacy against pests that have developed resistance. Consequently, there is a growing interest in adopting more sustainable control methods. Stevia rebaudiana is a valuable medicinal plant used in the food industry for the production of steviol glycosides, a type of natural sweetener. An EO that could be useful for creating innovative insecticides may come from the industrially used plant biomass. In this study, gas chromatography-mass spectrometry (GC-MS) was used to analyse the chemical composition of S. rebaudiana leaves. Sesquiterpenes, or caryophyllene oxide (20.7 %), spathulenol (14.9 %), and (E)-nerolidol (8.0 %), and diterpenes, or phytol (9.2 %), made up the majority of the EO composition. The efficacy of the EO major constituents, namely Phytol, (E)-nerolidol, Spathulenol, and Caryophyllene oxide, was also tested against S. frugiperda. Phytol was the most effective LC50 = 14.38 mg/L, followed by (E)-nerolidol LC50 = 15.88 mg/L, Spathulenol LC50 = 18.42 mg/L, and Caryophyllene oxide LC50 = 23.41 mg/L. Furthermore, some of the biological and histological features of the extracts were also studied in a lab setting. Overall, Stevia rebaudiana (Bertoni) should be given more consideration in the development of environmentally safe and efficient pesticides.
{"title":"Effective and eco-friendly botanical insecticidal agents against Spodoptera frugiperda (Noctuidae: Lepidoptera) using the essential oil of Stevia rebaudiana","authors":"Esmat A. El-Solimany , Antar A. Abdelhamid , Marwa Adel Thabet , Mohamed A. Gad","doi":"10.1016/j.napere.2024.100103","DOIUrl":"10.1016/j.napere.2024.100103","url":null,"abstract":"<div><div>Using synthetic pesticides is the main strategy for controlling pests. However, these compounds have caused worry because of their harmful effects on health and their diminishing efficacy against pests that have developed resistance. Consequently, there is a growing interest in adopting more sustainable control methods. <em>Stevia rebaudiana</em> is a valuable medicinal plant used in the food industry for the production of steviol glycosides, a type of natural sweetener. An EO that could be useful for creating innovative insecticides may come from the industrially used plant biomass. In this study, gas chromatography-mass spectrometry (GC-MS) was used to analyse the chemical composition of <em>S. rebaudiana</em> leaves. Sesquiterpenes, or caryophyllene oxide (20.7 %), spathulenol (14.9 %), and (<em>E</em>)-nerolidol (8.0 %), and diterpenes, or phytol (9.2 %), made up the majority of the EO composition. The efficacy of the EO major constituents, namely Phytol, (<em>E</em>)-nerolidol, Spathulenol, and Caryophyllene oxide, was also tested against <em>S. frugiperda.</em> Phytol was the most effective LC<sub>50</sub> = 14.38 mg/L, followed by (<em>E</em>)-nerolidol LC<sub>50</sub> = 15.88 mg/L, Spathulenol LC<sub>50</sub> = 18.42 mg/L, and Caryophyllene oxide LC<sub>50</sub> = 23.41 mg/L. Furthermore, some of the biological and histological features of the extracts were also studied in a lab setting. Overall, <em>Stevia rebaudiana</em> (Bertoni) should be given more consideration in the development of environmentally safe and efficient pesticides.</div></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"10 ","pages":"Article 100103"},"PeriodicalIF":0.0,"publicationDate":"2024-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142663868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-09DOI: 10.1016/j.napere.2024.100102
Abraham Akuoli Issaka Ndebugri , Joseph Xorxe Kugbe , Samuel Adu-Acheampong , Rosina Kyerematen
Botanicals have been encouraged as substitutes for conventional pesticides to reduce the impact of the latter on the environment. In line with that, we studied the effects of two plant extracts in controlling Sitophilus zeamais Motsch (Coleoptera: Curculionidae) attacks on stored maize (Zea mays L. Poales: Poaceae). The experiment was conducted under a normal room temperature of 27⁰C to 30⁰C and relative humidity of 74–76 % in a completely randomized experimental design. We infested the stored maize seeds in glass jars with S. zeamais F1 under a 12-hour day and 12-hour night within 24 hours period. This was after 50 g of the seeds were mixed with 2 g each of the two botanicals separately in different glass jars. There were 5 treatments and 10 replications in total. The data taken for this study were quantitative. To ascertain the efficacy of the botanicals we compared the number of dead and live weevils, of seeds damage and weight loss caused by weevil attacks under each of the different treatments by determining percentage mortality, percent weight loss, seed damage and progeny production. Our results showed that the different botanical formulations significantly controlled S. zeamais population resulting in reduced damage, better protection and improved storage of maize seeds. However, there were no significant differences in protections offered between shade dried neem seed powder and rice husk powder although the latter was the most efficacious with 85 % mortality compared to the former with 65 % with sundried neem seed powder being the least with a little above 40 % mortality. We concluded from this study that the formulated botanicals were very effective in controlling S. zeamais populations and therefore recommended that it be used as a cost effective and environmentally friendly protection of stored maize against S. zeamais attacks, especially in rural poor areas and places known for continuous seed production and storage, such as the case of the study community.
{"title":"Two plant extracts protect stored maize against infestation of Sitophilus zeamais in Northern Ghana","authors":"Abraham Akuoli Issaka Ndebugri , Joseph Xorxe Kugbe , Samuel Adu-Acheampong , Rosina Kyerematen","doi":"10.1016/j.napere.2024.100102","DOIUrl":"10.1016/j.napere.2024.100102","url":null,"abstract":"<div><div>Botanicals have been encouraged as substitutes for conventional pesticides to reduce the impact of the latter on the environment. In line with that, we studied the effects of two plant extracts in controlling <em>Sitophilus zeamais</em> Motsch (Coleoptera: Curculionidae) attacks on stored maize (<em>Zea mays</em> L. Poales: Poaceae). The experiment was conducted under a normal room temperature of 27⁰C to 30⁰C and relative humidity of 74–76 % in a completely randomized experimental design. We infested the stored maize seeds in glass jars with <em>S. zeamais</em> F<sub>1</sub> under a 12-hour day and 12-hour night within 24 hours period. This was after 50 g of the seeds were mixed with 2 g each of the two botanicals separately in different glass jars. There were 5 treatments and 10 replications in total. The data taken for this study were quantitative. To ascertain the efficacy of the botanicals we compared the number of dead and live weevils, of seeds damage and weight loss caused by weevil attacks under each of the different treatments by determining percentage mortality, percent weight loss, seed damage and progeny production. Our results showed that the different botanical formulations significantly controlled <em>S. zeamais</em> population resulting in reduced damage, better protection and improved storage of maize seeds. However, there were no significant differences in protections offered between shade dried neem seed powder and rice husk powder although the latter was the most efficacious with 85 % mortality compared to the former with 65 % with sundried neem seed powder being the least with a little above 40 % mortality. We concluded from this study that the formulated botanicals were very effective in controlling <em>S. zeamais</em> populations and therefore recommended that it be used as a cost effective and environmentally friendly protection of stored maize against <em>S. zeamais</em> attacks, especially in rural poor areas and places known for continuous seed production and storage, such as the case of the study community.</div></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"10 ","pages":"Article 100102"},"PeriodicalIF":0.0,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142663847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-29DOI: 10.1016/j.napere.2024.100096
Amal Hari , Rachid Lahlali , Ahmet Buğra Ortaakarsu , Noamane Taarji , Salah-Eddine Laasli , Khawla Karaaoui , Meryem Benjelloun , Wafae Ouchari , Mansour Sobeh , Ghizlane Echchgadda
Fusarium wilt, caused by Fusarium oxysporum, is one of the most devastating diseases to crops including potatoes. This fungus is widespread and dispersed via resistant spores. In this study, organic (methanol, hexane, and chloroform) and aqueous extracts of the aerial parts of wild Origanum elongatum were tested against mycelium and spores of F. oxysporum in both in-vitro (using the poisoned food technique) and in-planta assays. Further, the chemical composition of the extract was identified via HPLC-PDA-MS/MS. Then, molecular modeling was used to clarify the antifungal activity of identified biomolecules. The obtained results showed that organic and aqueous extracts showed significant antifungal and anti-sporulation activities with different effectiveness depending on the extract, concentration, and application test. In in-vitro assays of the aqueous extract, the highest antifungal activity was recorded at concentrations of 10 mg mL−1, while the highest anti-sporulation activity (91.79 %) was observed at 50 mg mL−1. In the organic extracts, methanol was the most effective against fungus with 100 % inhibition of mycelium at 5 mg mL −1 and the highest anti-sporulation effect (73.58 %) at 20 mg mL−1. In in-planta assays, the methanolic extract at 20 mg mL−1 achieved 0 % disease severity, while the aqueous extract at 50 mg mL−1 reduced severity to 7 %, both significantly more effective than the untreated control. Therefore, methanol was the most effective and these biological properties are supported by a wide range of bioactive molecules, including 56 molecules compared to only 28 molecules in the aqueous extract. These include glycosides of apigenin and kaempferol and salvianolic acid b. However, more research is needed to clarify the inhibition action of the individual components and their synergetic actions in controlled and field conditions.
{"title":"Inhibitory effects of wild Origanum elongatum extracts on Fusarium oxysporum mycelium growth and spores germination: Evidence from in-vitro, in-planta, and in-silico experiments","authors":"Amal Hari , Rachid Lahlali , Ahmet Buğra Ortaakarsu , Noamane Taarji , Salah-Eddine Laasli , Khawla Karaaoui , Meryem Benjelloun , Wafae Ouchari , Mansour Sobeh , Ghizlane Echchgadda","doi":"10.1016/j.napere.2024.100096","DOIUrl":"10.1016/j.napere.2024.100096","url":null,"abstract":"<div><div><em>Fusarium</em> wilt, caused by <em>Fusarium oxysporum,</em> is one of the most devastating diseases to crops including potatoes. This fungus is widespread and dispersed via resistant spores. In this study, organic (methanol, hexane, and chloroform) and aqueous extracts of the aerial parts of wild <em>Origanum elongatum</em> were tested against mycelium and spores of <em>F. oxysporum</em> in both <em>in-vitro</em> (using the poisoned food technique) and <em>in-planta</em> assays. Further, the chemical composition of the extract was identified <em>via</em> HPLC-PDA-MS/MS. Then, molecular modeling was used to clarify the antifungal activity of identified biomolecules. The obtained results showed that organic and aqueous extracts showed significant antifungal and anti-sporulation activities with different effectiveness depending on the extract, concentration, and application test. In <em>in-vitro</em> assays of the aqueous extract, the highest antifungal activity was recorded at concentrations of 10 mg mL<sup>−1</sup>, while the highest anti-sporulation activity (91.79 %) was observed at 50 mg mL<sup>−1</sup>. In the organic extracts, methanol was the most effective against fungus with 100 % inhibition of mycelium at 5 mg mL <sup>−1</sup> and the highest anti-sporulation effect (73.58 %) at 20 mg mL<sup>−1</sup>. In <em>in-planta</em> assays, the methanolic extract at 20 mg mL<sup>−1</sup> achieved 0 % disease severity, while the aqueous extract at 50 mg mL<sup>−1</sup> reduced severity to 7 %, both significantly more effective than the untreated control. Therefore, methanol was the most effective and these biological properties are supported by a wide range of bioactive molecules, including 56 molecules compared to only 28 molecules in the aqueous extract. These include glycosides of apigenin and kaempferol and salvianolic acid b. However, more research is needed to clarify the inhibition action of the individual components and their synergetic actions in controlled and field conditions.</div></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"10 ","pages":"Article 100096"},"PeriodicalIF":0.0,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142663869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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