Journal of Natural Pesticide Research最新文献

Alternative ecofriendly products to control vector borne diseases are urgently needed to combat mosquito related diseases. New modes of action, cost effectiveness, bio-degradability, and target specificity are some desirable attributes for these alternative products. Studies on macrofungi have become increasingly important for researchers in the current scenario. Macrofungi are widespread and are found all over the world. Basidiomycetes (mushrooms) are found mostly in warm and humid climatic conditions. No specific review is available on the basidiomycetes for mosquito control till 2022. A number of online libraries including PubMed, Scifinder, Google Scholar and Web of Science were used in searching for information on mushrooms for mosquito control. Natural antibiotics are abundant in basidiomycetes; they have shown antimicrobial, anticancer, antioxidant and other biological activities. This review summarises the potential role of mushrooms as promising and effective mosquito control agents.

Pollen beetles are a transcontinental threat to oilseed rape cultivation. Conventional insecticide use has resulted in resistance development, and can furthermore lead to losses of non-target organisms, including those that contribute to natural biological control of pollen beetle populations. Biosafe alternatives to synthetic pesticides must be explored. Double-stranded RNA (dsRNA) targeting pollen beetle, by the RNA interference (RNAi) mode of action, has been previously demonstrated under laboratory conditions. The present study examined, under a whole plant context, the potential for spray-induced gene silencing (SIGS) in pollen beetle. We confirmed translocation of sprayed dsRNA to later-emerging, non-sprayed dietary tissues of oilseed rape, as well as infiltration of sprayed dsRNA to the inner tissues of reproductive buds (pollen beetle’s food source). No effect on survival was observed. The inefficacy of SIGS observed here highlights the requirement for more effective SIGS approaches to pollen beetle management. The translocation of sprayed dsRNA within oilseed rape plants, and the non-significant yet detectable effect on target mRNA downregulation, together suggest potential for future optimization of a SIGS approach against pollen beetle.

Apple fruit is one of the worldwide most industrially valuable horticulture crops. However, the fruit is vulnerable to post-harvest brown rot degradation caused by Monilinia laxa and Monilinia fructigena, which is a prominent pre-and post-harvest disease of tree fruit. Numerous non-chemical post-harvest treatments have been investigated so far to control these pathogens to provide an alternative option to the synthetic fungicides that are commonly used and may have negative effects on human health and the environment. Therefore, this study aimed to assess the in vitro and in vivo efficacy at different concentrations of nine plants' aqueous extracts from Mentha pulegium, Citrus aurantium, Thymus vulgaris, Origanum compactum, Lavandula angustifolia, Syzygium aromaticum, Rosmarinus officinalis, Citrus sinensis and Eucalyptus radiate against the development of brown rot. The results demonstrated significant antifungal activities of all tested aqueous extracts on both fungi compared to the thiophanate-methyl fungicide (1 ppm) (100% inhibition). At the concentration of 1.5 mg/ml, S. aromaticum and O. compactum extracts were the most effective, showing significant inhibition values against M. laxa (91.06%) and against M. fructigena (90.02%). Interestingly, at higher concentration, spore germination of both Monilinia strains was significantly reduced with inhibition rate of 98.64% for M. laxa and 93.04% for M. fructigena. Also, high flavonoids and polyphenols contents were shown. Aqueous extracts of O. compactum, S. aromaticum, and T. vulgaris reduced the brown rot severity and incidence in vivo. The application of these plants' aqueous extracts on fruits seems to have a significant effect on fruit quality persistence, as indicated by weight loss, fruit firmness, total soluble solids, titrable acidity, and maturity index during 15 days of storage at 4 °C. The spectra analyzed from all tested plants revealed typical peaks and a large variety of functional groups such as C–H bonds, O–H groups, and C–C stretching. The plant’s aqueous extracts studied can be used as bio-fungicides against brown rot diseases.

Nano-emulsion containing Eucalyptus grandis (W. Hill ex Maiden) and Corymbia citrodora (Hook.) K.D.Hill & L.A.S.Johnson (common synonym Eucalyptus citriodora Hook.) leaf essential oils individual, combination was evaluated its larvicidal activity against Anopheles stephensi, Aedesaegypti and Culex quinquefasciatus mosquito larvae at 24 h post treatment. Nano-emulsion had low polydispersivity index and mean droplet size was below 66 nm. After 24 h E. grandis essential oil exhibited significant larvicidal activity with LC₅₀ and LC₉₀ values in A. aegypti (23.667; 438.797 ppm in A. aegypti A. stephensi (10.984; 277.535 ppm) and C. quinquefasciatus (4.676; 106.415 ppm). Similarly in C. citrodora showed low LC₅₀ and LC₉₀ values in A. aegypti (18.430; 1048.599 ppm), A. stephensi (6.573; 176.737 ppm) and C. quinquefasciatus (7.917; 287.720 ppm) respectively. The combination of E. grandis and C. citrodora essential oils showed higher mortality rates than individual essential oils, with low LC₅₀ and LC₉₀ values in A. aegypti (3.625; 58.962 ppm), A. stephensi (3.245; 34.473 ppm) and C. quinquefasciatus (3.002; 30.153 ppm). Nano-emulsion exhibited toxic effects similar to combination group with LC₅₀ and LC₉₀ values in A. aegypti (5.344; 113.362 ppm), A. stephensi (3.183; 83.112 ppm) and C. quinquefasciatus (2.832; 166.665 ppm). Chemical constituents of both essential oil were analyzed using GC-MS and FT-IR, result showed E. grandis contain different compounds namely; alpha-pinene, (31.74%); o-cymene, (17.84%); Eucalyptol (16.24%); Bicyclo[2.2.1]heptane (3.2%) and C. citrodora showed Citronellol (49.31%); dl-Isopulegol (25.39%) as a major compound while their synergistic action may be due to type’s composition of EOs might produce the synergist effect along with nanoemulsions. Our findings suggest that combination of essential oil E. grandis and C. citrodora can be used for mosquito control as green approach.

This study was conducted at Rangpur area of Bangladesh to evaluate the performance of Trichoderma harzianum based bio-fungicide against late blight and early blight of potato. A commercial fungicide, Agrizeb (Mancozeb) 80 WP was applied for comparison. These fungicides were applied in potato filed already infected by late and early blight. Lycomax found most effective for controlling both late blight and early blight while Agrizeb found potent for early blight. After applying Lycomax, late blight infection in plant has not increased rather decreased to 20.0% from the existing 22.67% and leaf infestation reduced to 10.67% from the existing 13.33%. In contrast for Agrizeb, plant infection increased to 71.67% from the prevailing 22.0% and leaf infection increased to 73% from the existing 14.67%. Thus, Lycomax confirmed 79.09% reduction of plant infection and 89.04% reduction of leaf infection compared to control which was 25.09% and 25%, respectively for Agrizeb. For early blight, same dose of Lycomax also reduce plant infection to 1.67% from existing 14.67% and leaf infection to 8.0% from prevailing 13.33%. Plant infection decreased to 5.0% from existing 15.0% and leaf infection 13.33% from 14.67% for Agrizeb. Therefore, Lycomax provided 98.19% control of plant infection and 90.16% control of leaf infection while Agrizeb provided 94.58% plant infection and 83.61% leaf infection over control. Moreover, Lycomax confirmed 21.66 ton/ha and 22.0 ton/ha of potato yield recovering the late blight and early blight diseases, respectively. These results conclude that Lycomax can effectively be applied to control late blight and early blight diseases.

Sinapis alba mustard seed meal extract is a by-product of mustard oil extraction, that is used for biodiesel and renewable jet fuel production. Recently, S. alba mustard seed meal has been shown to have herbicidal activity for a range of problematic weeds. The objectives of the present research were 1) to optimize the preparation of S. alba mustard seed meal extract; 2) to explore the applicability of S. alba mustard seed meal extract for control of liverwort, a common pest in ornamental plants greenhouses; and 3) to identify the major herbicidal compound(s) in these extracts. For extraction, methanol content in aqueous solution was optimized between 0% and 100%. Extracts prepared with 0–30% aqueous methanol provided 98–100% control of liverwort after single application and prevented liverwort plants from recovering. Extracts prepared using sequential extraction with 60% methanol provided an additional benefit of low particulate solutions. The efficiency of the produced S. alba mustard seed meal extract was comparable to two commercially available herbicides labeled for liverwort control under greenhouse conditions. Composition of S. alba mustard seed meal extracts was analyzed by high-performance liquid chromatography mass spectrometry and ion chromatography. 4-Hydroxybenzyl alcohol was identified as a main herbicidal compound based on the analysis and using commercially available reference solution. To the best of our knowledge, this is the first report of 4-hydroxybenzyl alcohol herbicidal activity toward nonvascular plants. The high concentrations of 4-hydroxybenzyl alcohol naturally produced from S. alba mustard seed meal, makes it a potential herbicide feedstock material.

Bean anthracnose results in significant yield losses, and the present primary management strategy of using synthetic fungicides is hampered by a number of factors. This study assessed the antifungal effects of aqueous, methanolic, and ethyl acetate extracts of Leonotis nepetifolia (Lamiaceae) against Colletotrichum species that cause bean anthracnose. The poisoned food technique was used to evaluate extracts in-vitro, and trials were carried out on farmer's plots. On the other hand, phytochemical analysis was done using LC-MS. Results obtained in-vitro show that the ethyl acetate extract was the most effective since it completely inhibited mycelial development at concentrations of 5 mg/mL and 2.5 mg/mL. On the other hand, the aqueous and methanolic extracts exhibited a comparable inhibitory effect, and at the higher concentration (5.0 mg/mL), a remarkable percentage of inhibition of 73.3% and 83.1% was recorded. According to field trials, bean anthracnose incidence was low and there were fewer infected pods per plant in plots treated with L. nepetifolia extracts than in the negative control. In comparison to the negative control plots, extracts-treated plots had larger weights of 100 seeds but no discernible negative effect in seed germination. LC-MS analysis indicated the presence of Linoleoyl ethanolamide, Curcumol, Deguelin, 9,10-dihydroxy-12Z-octadecenoic acid, Naringenin-7-O-glucoside, and 9S-hydroxy-10e 12z 15z-octadecatrienoic acid in the ethyl acetate extract whereas 6-Methoxyluteolin, Genistein, 5,7,3,4-tetramethoxyisoflavone, and 3,4-Dihydroxymandelic acid were identified in the aqueous extract. Antifungal activity and the presence of bioactive components revealed by extracts in this study demonstrate the potential of L. nepetifolia as a source of botanical fungicide for the management of bean anthracnose.

With a growing global population, the demand for food, clothing and shelter became crucial for sustaining life. Moreover, the COVID-19 pandemic/endemic, along with the threat of other endemics, posed significant challenges in ensuring the delivery of nutritionally rich food. To address this, the World Health Organization (WHO) recommended the ban of some highly toxic chemical pesticides. Excessive consumption and exposure to pesticides possibly contributed to a decline in the human immunity levels making our fight against the pandemics more difficult and challenging. During the lockdown COVID-19 crisis, we conducted a survey to gather insights from farm landers (FLs), garden lovers (GLs), domestic front users (DFUs) predominantly from India, but also from other regions worldwide. The survey aimed at better understanding the usage of pesticides, both chemicals (CPs), bio-pesticides (BPs) or both, and their global utilization. A statistical survey with seven rudimentary sections was designed to receive the inputs (elementary inputs, awareness and perception, pesticide utility, health, ecology, and alternatives for safer trials) with fairness and care. The data was statistically analyzed within each group of population, revealing significant variations within the groups. Our study indicated that FLs (44.7 %) predominantly used BPs, GLs (18.8 %) relied on CPs, while DFUs (100.0 %) used a combination of both without any specific intention. Correlation analysis, given by R (correlation coefficient) and p (probability of obtaining an equal or more extreme effect than the found considering the null hypothesis as true) values revealed the existence of a positive and significant relationship between the selected variables, such as level of education (LOE) and gender, with the adoption of new alternatives (2loglikelihood = 64.743, with χ² = 128.4, degrees of freedom (df) = 24, p<*0.05, **0.01, and *** 0.001) through multinomial regression analysis, indicating the fitness of the model. Likewise, our study primarily focused on exploring the insights for the development of nano-biopesticides, as improved alternatives to the existing solutions.

Among the poaceae family, cereals are known as the most important products because they are the main source of energy and food. Different plant pathogens, including oomycetes, fungi, bacteria, nematodes and viruses, can infect cereal products. Fungal diseases are the most important group of cereal pathogens, causing significant crop losses worldwide. Several techniques, including chemical control, the use of resistant cultivars, biological control, and application of antimicrobial plant metabolites can be used to manage cereal diseases. The use of chemical products is limited due to undesirable environmental effects and the emergence of resistant pathogens to fungicides. Due to the fact that medicinal plants produce a variety of secondary metabolites with antimicrobial effects, they are considered as an alternative to chemical substances. In this study, we have discussed the sensitivity of most important fungal and bacterial pathogens of cereal crops against different essential oils and their main components, together with their modes of action in controlling cereal diseases.