Orhan Dengiz, Nursaç Serda Kaya, Wudu Abiye, Endalamaw Dessie Alebachew
Intensive agricultural practices to meet food demand have led to a decline in soil quality and agricultural productivity, posing significant challenges to environmental sustainability. Consequently, the present research focused on the development of models based on artificial intelligence techniques to predict the soil quality index (SQI) for soybean (Glycine max) cultivation using a total of 89 soil samples taken at 300-m grit system at depths of 0–20 cm. A set of 28 parameters categorized into main physical, chemical (organic matter, pH, EC, etc.), fertility (macro- and micronutrient elements), and biological (soil respiration, metabolic coefficient, and microbial biomass carbon) parameters were used for the total dataset (TDS). The minimum dataset (MDS), which consisted of the most sensitive parameters, was selected using principal component analysis. In this study, SQI was calculated for both TDS and MDS using a neutrosophic fuzzy analytic hierarchy process and standard scoring function. The resulting SQITDS and SQIMDS values were then predicted using machine learning approaches, including multiple linear regression (MLR) and random forest regression (RFR). The accuracy of these predictions was then examined using various metrics such as mean absolute error, mean squared error, and root mean square error. The results show that MLR outperforms RFR for both SQITDS and SQIMDS with significantly lower error indices and higher R2 values than RFR through 10-fold cross-validation. In addition, this study statistically compared the obtained SQITDS and SQIMDS values with normalized difference vegetation index (NDVI) values derived from the Sentinel-2A satellite for May 2021. The same satisfactory R2 values (0.84) were obtained by statistically comparing both SQITDS and SQIMDS with NDVI values. Furthermore, this study demonstrates the effective integration of advanced techniques such as machine learning models with remote sensing and geographic information system technologies, for the analysis and processing of both original and generated information in the vast domain of SQI.
{"title":"Enhancing the soil quality index model based on neutrosophic fuzzy-AHP integrated with remote sensing and artificial intelligence technique","authors":"Orhan Dengiz, Nursaç Serda Kaya, Wudu Abiye, Endalamaw Dessie Alebachew","doi":"10.1002/saj2.70133","DOIUrl":"https://doi.org/10.1002/saj2.70133","url":null,"abstract":"<p>Intensive agricultural practices to meet food demand have led to a decline in soil quality and agricultural productivity, posing significant challenges to environmental sustainability. Consequently, the present research focused on the development of models based on artificial intelligence techniques to predict the soil quality index (SQI) for soybean (<i>Glycine max</i>) cultivation using a total of 89 soil samples taken at 300-m grit system at depths of 0–20 cm. A set of 28 parameters categorized into main physical, chemical (organic matter, pH, EC, etc.), fertility (macro- and micronutrient elements), and biological (soil respiration, metabolic coefficient, and microbial biomass carbon) parameters were used for the total dataset (TDS). The minimum dataset (MDS), which consisted of the most sensitive parameters, was selected using principal component analysis. In this study, SQI was calculated for both TDS and MDS using a neutrosophic fuzzy analytic hierarchy process and standard scoring function. The resulting SQI<sub>TDS</sub> and SQI<sub>MDS</sub> values were then predicted using machine learning approaches, including multiple linear regression (MLR) and random forest regression (RFR). The accuracy of these predictions was then examined using various metrics such as mean absolute error, mean squared error, and root mean square error. The results show that MLR outperforms RFR for both SQI<sub>TDS</sub> and SQI<sub>MDS</sub> with significantly lower error indices and higher <i>R</i><sup>2</sup> values than RFR through 10-fold cross-validation. In addition, this study statistically compared the obtained SQI<sub>TDS</sub> and SQI<sub>MDS</sub> values with normalized difference vegetation index (NDVI) values derived from the Sentinel-2A satellite for May 2021. The same satisfactory <i>R<sup>2</sup></i> values (0.84) were obtained by statistically comparing both SQI<sub>TDS</sub> and SQI<sub>MDS</sub> with NDVI values. Furthermore, this study demonstrates the effective integration of advanced techniques such as machine learning models with remote sensing and geographic information system technologies, for the analysis and processing of both original and generated information in the vast domain of SQI.</p>","PeriodicalId":101043,"journal":{"name":"Proceedings - Soil Science Society of America","volume":"89 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145146846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Christopher J. Hawxhurst, Travis McClure, Daniel Kirk, Mikhael Shor, Daniel J. Gage, Leslie M. Shor
Chemicals are an integral part of modern agriculture and are applied through a variety of methods. Some agrochemicals used for crop protection are absorbed by the roots prior to translocation to the rest of the plant. To be absorbed by the root, the agrochemical must first be transported through the soil, often by water. Some agrochemicals suffer from poor water-based soil transmission due to their chemical properties, limiting their application as a traditional seed treatment. Two agrochemicals with poor water-based soil transmission are Chlorantraniliprole and Spinosad. Soil protists are an important component of the soil microbial community. Certain soil protists have been shown to facilitate the transport and target delivery of suspended particles and bacteria through soil and soil-like structures. Here, we provide practical evidence that a soil protist, Colpoda sp., when co-inoculated with an agrochemical seed treatment, can substantially and robustly reduce subsequent pest feeding damage versus application of the agrochemical alone. Using maize (Zea mays L.) and fall armyworm, Spodoptera frugiperda (J. E. Smith, 1797) (Lepidoptera: Noctuidae), in a plant damage assay, we compare pest feeding damage and pest mortality for leaf samples from plants whose seeds were treated with only protists, only agrochemical, or agrochemical + protists. We discover, for both agrochemicals tested, that co-inoculation of protists with agrochemical increases protection in leaves versus agrochemical alone. Protist amendment is a simple, natural, chemical-free, soil-based transport enhancer that may be widely useful in a variety of contexts including more sustainable agriculture methods and cost-effective integrated pest management.
化学品是现代农业的一个组成部分,并通过各种方法应用。一些用于作物保护的农用化学品在转运到植物的其他部分之前被根部吸收。为了被根部吸收,农药必须首先通过土壤运输,通常是通过水。由于一些农用化学品的化学性质,它们的水基土壤传输能力差,限制了它们作为传统种子处理的应用。两种水基土壤传输性差的农用化学品是氯虫腈和Spinosad。土壤原生生物是土壤微生物群落的重要组成部分。某些土壤原生生物已被证明可以通过土壤和类似土壤的结构促进悬浮颗粒和细菌的运输和目标递送。在这里,我们提供了实际的证据,当与农化种子处理共接种土壤原生生物时,与单独施用农化剂相比,可以显著减少随后的害虫取食损害。以玉米(Zea mays L.)和夜蛾(Spodoptera frugiperda, J. E. Smith, 1797)(鳞翅目:夜蛾科)为研究对象,比较了仅用原生生物、仅用农化剂和农化+原生生物处理的植物叶片样品的取食损害和病虫害死亡率。我们发现,对于两种农用化学品测试,与单独农用化学品相比,原生生物与农用化学品共接种增加了对叶片的保护。原生植物改良剂是一种简单、天然、不含化学物质、基于土壤的运输增强剂,可广泛用于各种情况,包括更可持续的农业方法和具有成本效益的综合虫害管理。
{"title":"Addition of soil protists may enhance performance of agrochemical seed treatments","authors":"Christopher J. Hawxhurst, Travis McClure, Daniel Kirk, Mikhael Shor, Daniel J. Gage, Leslie M. Shor","doi":"10.1002/saj2.70132","DOIUrl":"https://doi.org/10.1002/saj2.70132","url":null,"abstract":"<p>Chemicals are an integral part of modern agriculture and are applied through a variety of methods. Some agrochemicals used for crop protection are absorbed by the roots prior to translocation to the rest of the plant. To be absorbed by the root, the agrochemical must first be transported through the soil, often by water. Some agrochemicals suffer from poor water-based soil transmission due to their chemical properties, limiting their application as a traditional seed treatment. Two agrochemicals with poor water-based soil transmission are Chlorantraniliprole and Spinosad. Soil protists are an important component of the soil microbial community. Certain soil protists have been shown to facilitate the transport and target delivery of suspended particles and bacteria through soil and soil-like structures. Here, we provide practical evidence that a soil protist, <i>Colpoda</i> sp., when co-inoculated with an agrochemical seed treatment, can substantially and robustly reduce subsequent pest feeding damage versus application of the agrochemical alone. Using maize (<i>Zea mays</i> L.) and fall armyworm, <i>Spodoptera frugiperda</i> (J. E. Smith, 1797) (Lepidoptera: Noctuidae), in a plant damage assay, we compare pest feeding damage and pest mortality for leaf samples from plants whose seeds were treated with only protists, only agrochemical, or agrochemical + protists. We discover, for both agrochemicals tested, that co-inoculation of protists with agrochemical increases protection in leaves versus agrochemical alone. Protist amendment is a simple, natural, chemical-free, soil-based transport enhancer that may be widely useful in a variety of contexts including more sustainable agriculture methods and cost-effective integrated pest management.</p>","PeriodicalId":101043,"journal":{"name":"Proceedings - Soil Science Society of America","volume":"89 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://acsess.onlinelibrary.wiley.com/doi/epdf/10.1002/saj2.70132","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145146845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Enhancing crop productivity and soil sustainability under climate-smart agriculture involves strategically using soil amendments to improve soil health and resilience. A field experiment at Prairie View A&M University, Texas, studied the effects of soil amendments (chicken and dairy manures and biochar) on some soil health indicators. The experiment used two biochar rates (2268 and 4536 kg ha−1) and two types of manure (chicken and dairy) at three rates (0, 224, and 448 kg total N ha−1 for sweet corn [Zea mays (L.)] and 0, 180, and 360 kg N ha−1 for sorghum [Sorghum bicolor (L.) Moench]) in a factorial design with three replications. Soil macronutrients and micronutrients were measured as chemical soil health indicators, and bulk density, porosity, and saturated hydraulic conductivity were measured as physical soil health indicators. Dairy manure significantly increased soil calcium (Ca) and potassium (K) concentrations. Higher manure application rates improved soil nutrient concentration, with the highest phosphorus (P), Ca, magnesium (Mg), and manganese (Mn) concentration levels at the double recommended rate. Biochar did not affect nutrient concentration but improved soil physical properties by increasing porosity, hydraulic conductivity, and reducing bulk density, especially at higher rates in sweet corn. Correlation analysis showed bulk density was negatively correlated with key nutrients like potassium (K), Ca, and Mg, while porosity and hydraulic conductivity positively influenced nutrient availability. The principal component analysis highlighted that sweet corn and sorghum respond positively to selected soil amendments, while their specific impacts vary based on crop type. The findings emphasize balancing manure and biochar application rates to optimize soil fertility and minimize environmental risks, supporting sustainable soil management strategies.
在气候智能型农业下提高作物生产力和土壤可持续性涉及战略性地利用土壤改良剂来改善土壤健康和恢复力。德克萨斯州Prairie View a&m大学的一项田间试验研究了土壤改良剂(鸡粪、牛粪和生物炭)对一些土壤健康指标的影响。试验采用2种生物炭(2268和4536 kg ha - 1)和2种粪肥(鸡粪和乳肥),在甜玉米(Zea mays (L.))和高粱(sorghum (L.))中分别以3种氮肥(0、224和448 kg N ha - 1)和0、180和360 kg N ha - 1施用。[Moench]])在三次重复的析因设计中。测定土壤宏量和微量元素作为土壤化学健康指标,测定容重、孔隙度和饱和导水率作为土壤物理健康指标。牛粪显著提高了土壤钙、钾浓度。较高的施肥量提高了土壤养分浓度,在双倍推荐施肥量下,磷(P)、钙、镁(Mg)和锰(Mn)浓度最高。生物炭不影响养分浓度,但通过增加孔隙度、导水率和降低容重来改善土壤的物理性质,尤其是在甜玉米中。相关分析表明,容重与钾、钙、镁等关键养分呈负相关,孔隙度和导水率与养分有效性呈正相关。主成分分析表明,甜玉米和高粱对土壤改良剂有积极的响应,但其具体影响因作物类型而异。研究结果强调平衡肥料和生物炭的施用量,以优化土壤肥力,最大限度地降低环境风险,支持可持续土壤管理战略。
{"title":"Improving soil health through manure and biochar amendments under climate-smart agriculture","authors":"Binita Thapa, Ripendra Awal, Ali Fares, Atikur Rahman, Anoop Veettil, Almoutaz Elhassan, Niraj KC","doi":"10.1002/saj2.70129","DOIUrl":"10.1002/saj2.70129","url":null,"abstract":"<p>Enhancing crop productivity and soil sustainability under climate-smart agriculture involves strategically using soil amendments to improve soil health and resilience. A field experiment at Prairie View A&M University, Texas, studied the effects of soil amendments (chicken and dairy manures and biochar) on some soil health indicators. The experiment used two biochar rates (2268 and 4536 kg ha<sup>−1</sup>) and two types of manure (chicken and dairy) at three rates (0, 224, and 448 kg total N ha<sup>−1</sup> for sweet corn [<i>Zea mays</i> (L.)] and 0, 180, and 360 kg N ha<sup>−1</sup> for sorghum [<i>Sorghum bicolor</i> (L.) Moench]) in a factorial design with three replications. Soil macronutrients and micronutrients were measured as chemical soil health indicators, and bulk density, porosity, and saturated hydraulic conductivity were measured as physical soil health indicators. Dairy manure significantly increased soil calcium (Ca) and potassium (K) concentrations. Higher manure application rates improved soil nutrient concentration, with the highest phosphorus (P), Ca, magnesium (Mg), and manganese (Mn) concentration levels at the double recommended rate. Biochar did not affect nutrient concentration but improved soil physical properties by increasing porosity, hydraulic conductivity, and reducing bulk density, especially at higher rates in sweet corn. Correlation analysis showed bulk density was negatively correlated with key nutrients like potassium (K), Ca, and Mg, while porosity and hydraulic conductivity positively influenced nutrient availability. The principal component analysis highlighted that sweet corn and sorghum respond positively to selected soil amendments, while their specific impacts vary based on crop type. The findings emphasize balancing manure and biochar application rates to optimize soil fertility and minimize environmental risks, supporting sustainable soil management strategies.</p>","PeriodicalId":101043,"journal":{"name":"Proceedings - Soil Science Society of America","volume":"89 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://acsess.onlinelibrary.wiley.com/doi/epdf/10.1002/saj2.70129","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145135515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Galloway, L. A., Gamble, A. V., Guertal, E. A., Feng, Y., & Ogles, C. Z. (2025). Potential of ammonium thiosulfate and potassium thiosulfate to inhibit nitrification in soils. Soil Science Society of America Journal, 89, e70053. https://doi.org/10.1002/saj2.70053
In the first sentence of the fifth paragraph of the Introduction section, the sentence “Ammonium thiosulfate (ATS) and potassium thiosulfate (KTS) are used as liquid fertilizers.” should read, “Ammonium thiosulfate (ATS) and potassium thiosulfate (KTS) are used as liquid fertilizers (KTS is a registered trademark of Tessenderlo Kerley, Inc.).” to acknowledge the legal trademark status of this fertilizer.
{"title":"Correction to “Potential of ammonium thiosulfate and potassium thiosulfate to inhibit nitrification in soils”","authors":"","doi":"10.1002/saj2.70128","DOIUrl":"10.1002/saj2.70128","url":null,"abstract":"<p>Galloway, L. A., Gamble, A. V., Guertal, E. A., Feng, Y., & Ogles, C. Z. (2025). Potential of ammonium thiosulfate and potassium thiosulfate to inhibit nitrification in soils. <i>Soil Science Society of America Journal</i>, 89, e70053. https://doi.org/10.1002/saj2.70053</p><p>In the first sentence of the fifth paragraph of the Introduction section, the sentence “Ammonium thiosulfate (ATS) and potassium thiosulfate (KTS) are used as liquid fertilizers.” should read, “Ammonium thiosulfate (ATS) and potassium thiosulfate (KTS) are used as liquid fertilizers (KTS is a registered trademark of Tessenderlo Kerley, Inc.).” to acknowledge the legal trademark status of this fertilizer.</p><p>We apologize for this error.</p>","PeriodicalId":101043,"journal":{"name":"Proceedings - Soil Science Society of America","volume":"89 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://acsess.onlinelibrary.wiley.com/doi/epdf/10.1002/saj2.70128","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145111323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ziyi Gu, Yang Yang, Tingting Peng, Hui Zhang, Xin Wei, Yingna Liu
Slope aspect is a significant terrain attribute influencing soil physical and chemical processes. Yet its impact on soil quality and erosion has rarely been studied on gently sloping farmlands. The objective was to evaluate the effects of slope aspect on soil quality and its response to soil erosion in the black soil region of northeast China, a temperate environment featuring gently sloping farmlands. Over a nearly north-south symmetric sloping farmland spanning ∼3500 m, fifteen soil physical and chemical properties were investigated at every 40 m, and an integrated soil quality index (SQI) was calculated combining principal component analysis and scoring functions. The mean annual erosion rate (ER) was estimated using the cesium-137 tracing technique. Compared to the south-facing slope, the north-facing slope possessed significantly lower pH and higher saturated hydraulic conductivity, sand content, and almost all the essential nutrient contents, therefore overall better soil quality (p < 0.05). No statistical difference was spotted in ER between the two slopes (p > 0.05); however, erosion was found to deteriorate soil quality via distinct pathways. On the north-facing slope, erosion affected SQI predominantly through its negative impact on soil organic carbon content and wet-aggregate stability, and conservation tillage practices were suggested. However, on the south-facing slope, the detrimental influence was primarily driven through the depletion of soil nutrient contents, particularly available phosphorus and total nitrogen, and contour tillage and hedgerows were strongly recommended. These findings hold important practical implications for agricultural management in temperate environments.
坡向是影响土壤理化过程的重要地形属性。然而,其对土壤质量和侵蚀的影响很少在缓坡农田上进行研究。以东北黑土区为研究对象,研究了不同坡向对土壤质量的影响及其对土壤侵蚀的响应。在跨度约3500 m的近南北对称坡耕地上,研究了每40 m 15个土壤理化性质,并结合主成分分析和评分函数计算了综合土壤质量指数(SQI)。利用铯-137示踪技术估算了平均年侵蚀速率(ER)。与南坡相比,北坡的pH值显著降低,饱和导水率、含砂量和几乎所有必需养分含量均较高,整体土壤质量较好(p < 0.05)。两组间ER差异无统计学意义(p > 0.05);然而,发现侵蚀通过不同的途径使土壤质量恶化。北坡侵蚀主要通过对土壤有机碳含量和湿团聚体稳定性的负面影响来影响土壤质量指数,建议采取保护性耕作措施。然而,在朝南的斜坡上,有害影响主要是由于土壤养分含量的消耗,特别是有效磷和全氮的消耗,因此强烈建议采用等高线耕作和树篱。这些发现对温带环境下的农业管理具有重要的实际意义。
{"title":"Slope aspect affects soil quality and its response to soil erosion on temperate gently sloping farmlands","authors":"Ziyi Gu, Yang Yang, Tingting Peng, Hui Zhang, Xin Wei, Yingna Liu","doi":"10.1002/saj2.70130","DOIUrl":"10.1002/saj2.70130","url":null,"abstract":"<p>Slope aspect is a significant terrain attribute influencing soil physical and chemical processes. Yet its impact on soil quality and erosion has rarely been studied on gently sloping farmlands. The objective was to evaluate the effects of slope aspect on soil quality and its response to soil erosion in the black soil region of northeast China, a temperate environment featuring gently sloping farmlands. Over a nearly north-south symmetric sloping farmland spanning ∼3500 m, fifteen soil physical and chemical properties were investigated at every 40 m, and an integrated soil quality index (SQI) was calculated combining principal component analysis and scoring functions. The mean annual erosion rate (ER) was estimated using the cesium-137 tracing technique. Compared to the south-facing slope, the north-facing slope possessed significantly lower pH and higher saturated hydraulic conductivity, sand content, and almost all the essential nutrient contents, therefore overall better soil quality (<i>p</i> < 0.05). No statistical difference was spotted in ER between the two slopes (<i>p</i> > 0.05); however, erosion was found to deteriorate soil quality via distinct pathways. On the north-facing slope, erosion affected SQI predominantly through its negative impact on soil organic carbon content and wet-aggregate stability, and conservation tillage practices were suggested. However, on the south-facing slope, the detrimental influence was primarily driven through the depletion of soil nutrient contents, particularly available phosphorus and total nitrogen, and contour tillage and hedgerows were strongly recommended. These findings hold important practical implications for agricultural management in temperate environments.</p>","PeriodicalId":101043,"journal":{"name":"Proceedings - Soil Science Society of America","volume":"89 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145111322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Elahe Daraei, Hossein Bayat, Jiří Šimůnek, Andrew S. Gregory
Clay nanoparticles (NPs) are recognized as natural soil amendments. However, the effects of different types of clay NPs and their application rates on the physical, chemical, and biological characteristics of soils, solute transport, and plant photosynthesis parameters have not been thoroughly investigated. This study focused on amending two soil textures—sandy loam and loam—by adding 3% nano clay. The original and amended soils were packed into soil columns to conduct cultivation experiments with quinoa (Chenopodium quinoa Willd) plants and displacement solute transport experiments. The goal of column experiments was to explore the impact of the nano clay amendment on the photosynthetic properties of quinoa plants and solute transport in soils. The results indicated that adding NPs to loam soil improved photosynthesis and stomatal conductance. Additionally, the introduction of nano clays reduced sub-stomatal CO2 levels in the amended soils compared to the control soils. In sandy loam soil, both with and without cultivation, the addition of nano clay enhanced saturated hydraulic conductivity, dispersivity, and maximum chloride concentration when compared to the control. However, it also resulted in a decrease in immobile water content and a reduction in peak travel time. In loam soil, the application of nano clay—regardless of cultivation method—increased dispersivity and immobile water contents while reducing maximum chloride concentration. It simultaneously decreased hydraulic conductivity compared to control conditions and also increased it in some instances. This research demonstrates that the nano clay amendment significantly alters soil's physical and chemical properties, affecting solute transport and the photosynthetic parameters of the quinoa cultivar.
{"title":"Evaluating the effects of montmorillonite amendments on quinoa growth, water flow, and solute transport in sandy loam and loam soils","authors":"Elahe Daraei, Hossein Bayat, Jiří Šimůnek, Andrew S. Gregory","doi":"10.1002/saj2.70117","DOIUrl":"10.1002/saj2.70117","url":null,"abstract":"<p>Clay nanoparticles (NPs) are recognized as natural soil amendments. However, the effects of different types of clay NPs and their application rates on the physical, chemical, and biological characteristics of soils, solute transport, and plant photosynthesis parameters have not been thoroughly investigated. This study focused on amending two soil textures—sandy loam and loam—by adding 3% nano clay. The original and amended soils were packed into soil columns to conduct cultivation experiments with quinoa (Chenopodium quinoa Willd) plants and displacement solute transport experiments. The goal of column experiments was to explore the impact of the nano clay amendment on the photosynthetic properties of quinoa plants and solute transport in soils. The results indicated that adding NPs to loam soil improved photosynthesis and stomatal conductance. Additionally, the introduction of nano clays reduced sub-stomatal CO<sub>2</sub> levels in the amended soils compared to the control soils. In sandy loam soil, both with and without cultivation, the addition of nano clay enhanced saturated hydraulic conductivity, dispersivity, and maximum chloride concentration when compared to the control. However, it also resulted in a decrease in immobile water content and a reduction in peak travel time. In loam soil, the application of nano clay—regardless of cultivation method—increased dispersivity and immobile water contents while reducing maximum chloride concentration. It simultaneously decreased hydraulic conductivity compared to control conditions and also increased it in some instances. This research demonstrates that the nano clay amendment significantly alters soil's physical and chemical properties, affecting solute transport and the photosynthetic parameters of the quinoa cultivar.</p>","PeriodicalId":101043,"journal":{"name":"Proceedings - Soil Science Society of America","volume":"89 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145101566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Extracellular enzymes play a key role in microbe-mediated organic matter decomposition in soils, and the efficiency of these enzymes in substrate decomposition depends on their mobility and specific activity in soils. In this work, we explored the influence of biochar adsorption on extracellular enzyme activity across a spectrum of environmental conditions, from simple to complex. Batch adsorption results showed that biochar adsorption of two hydrolytic enzymes—α-amylase and amyloglucosidase (AMG)—similarly decreases with pH and follows the Langmuir isotherm, suggesting electrostatic interaction between them. Activity of AMG and alkaline phosphatase (ALP), which belong to carbon and phosphorus cycling enzymes, was measured using a novel calorimetric method. The technique demonstrated advantages over conventional enzyme assays, such as in situ real-time measurement of reaction rate and the ability to identify potential interferences. The technique enabled the measurement of specific activity of biochar-adsorbed AMG, which ranged from 10% to 90% of that of free AMG. The effect of substrate adsorption on activity measurement was demonstrated through the examination of two substrates for ALP, which suggested the use of effective substrate concentration (instead of nominal concentration) in calculating enzyme activity kinetics. Soil column experiments showed that biochar amendment can affect the activity of AMG in starch hydrolysis through changing the mobility of AMG (and accessibility to substrate) and its specific activity. Results from this work improve our understanding of the effects of biochar adsorption on enzyme activity and suggest the need to appropriately interpret enzyme activity data and account for confounding processes.
{"title":"Effects of biochar adsorption on extracellular enzymes activity: measurement and interpretation","authors":"Lingqun Zeng, Jeremy Feldblyum, Rixiang Huang","doi":"10.1002/saj2.70123","DOIUrl":"10.1002/saj2.70123","url":null,"abstract":"<p>Extracellular enzymes play a key role in microbe-mediated organic matter decomposition in soils, and the efficiency of these enzymes in substrate decomposition depends on their mobility and specific activity in soils. In this work, we explored the influence of biochar adsorption on extracellular enzyme activity across a spectrum of environmental conditions, from simple to complex. Batch adsorption results showed that biochar adsorption of two hydrolytic enzymes—α-amylase and amyloglucosidase (AMG)—similarly decreases with pH and follows the Langmuir isotherm, suggesting electrostatic interaction between them. Activity of AMG and alkaline phosphatase (ALP), which belong to carbon and phosphorus cycling enzymes, was measured using a novel calorimetric method. The technique demonstrated advantages over conventional enzyme assays, such as in situ real-time measurement of reaction rate and the ability to identify potential interferences. The technique enabled the measurement of specific activity of biochar-adsorbed AMG, which ranged from 10% to 90% of that of free AMG. The effect of substrate adsorption on activity measurement was demonstrated through the examination of two substrates for ALP, which suggested the use of effective substrate concentration (instead of nominal concentration) in calculating enzyme activity kinetics. Soil column experiments showed that biochar amendment can affect the activity of AMG in starch hydrolysis through changing the mobility of AMG (and accessibility to substrate) and its specific activity. Results from this work improve our understanding of the effects of biochar adsorption on enzyme activity and suggest the need to appropriately interpret enzyme activity data and account for confounding processes.</p>","PeriodicalId":101043,"journal":{"name":"Proceedings - Soil Science Society of America","volume":"89 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145101549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hydroxyapatite is an important phosphorus (P) sink in calcareous soils. The activity of carbonate in soil pore water, however, is often underestimated because soil respiration and solution-calcite equilibria could elevate CO2(g) concentration much greater than 415 ppmv (i.e., pCO2: ∼0.3 mm Hg). Thus far, the role of CO2(g) or pCO2 in the hydroxyapatite formation in calcareous soils has not been extensively investigated. Accordingly, the effects of CO2 concentration (415, 8000, and 20,000 ppmv) on hydroxyapatite formation were investigated at pH 8 using experimental geochemistry and X-ray diffraction (XRD) analysis. XRD analyses showed the formation of hydroxyapatite under all CO2 concentrations, but the extent of calcite formation increased with increasing CO2 concentration. The formation of calcium (Ca) carbonate phosphate was also observed after 30 days under [CO2(g)] up to 8000 ppmv. This is attributed to an increase in calcium carbonate formation. Scanning electron microscopy showed rounded hydroxyapatite particles. The variability of [CO2(g)] in subsoils should be considered in the P cycle in calcareous soils.
羟基磷灰石是钙质土壤中重要的磷汇。然而,土壤孔隙水中碳酸盐的活性常常被低估,因为土壤呼吸和溶液-方解石平衡可以使CO2(g)浓度大大高于415 ppmv(即pCO2: ~ 0.3 mm Hg)。迄今为止,CO2(g)或pCO2在钙质土壤中羟基磷灰石形成中的作用尚未得到广泛的研究。因此,采用实验地球化学和x射线衍射(XRD)分析,研究了pH为8时CO2浓度(415、8000和20000 ppmv)对羟基磷灰石形成的影响。XRD分析表明,在所有CO2浓度下均可形成羟基磷灰石,但方解石的形成程度随CO2浓度的增加而增加。在高达8000 ppmv的[CO2(g)]下30天后,还观察到碳酸钙(Ca)磷酸盐的形成。这是由于碳酸钙地层的增加。扫描电镜显示圆形羟基磷灰石颗粒。在钙质土壤的磷循环中,应考虑底土[CO2(g)]的变异性。
{"title":"Effects of pCO2 on hydroxyapatite formation: X-ray diffraction study","authors":"Shravani Kalita, Ai Chen, Yuji Arai","doi":"10.1002/saj2.70126","DOIUrl":"10.1002/saj2.70126","url":null,"abstract":"<p>Hydroxyapatite is an important phosphorus (P) sink in calcareous soils. The activity of carbonate in soil pore water, however, is often underestimated because soil respiration and solution-calcite equilibria could elevate CO<sub>2</sub>(g) concentration much greater than 415 ppmv (i.e., pCO<sub>2</sub>: ∼0.3 mm Hg). Thus far, the role of CO<sub>2</sub>(g) or pCO<sub>2</sub> in the hydroxyapatite formation in calcareous soils has not been extensively investigated. Accordingly, the effects of CO<sub>2</sub> concentration (415, 8000, and 20,000 ppmv) on hydroxyapatite formation were investigated at pH 8 using experimental geochemistry and X-ray diffraction (XRD) analysis. XRD analyses showed the formation of hydroxyapatite under all CO<sub>2</sub> concentrations, but the extent of calcite formation increased with increasing CO<sub>2</sub> concentration. The formation of calcium (Ca) carbonate phosphate was also observed after 30 days under [CO<sub>2</sub>(g)] up to 8000 ppmv. This is attributed to an increase in calcium carbonate formation. Scanning electron microscopy showed rounded hydroxyapatite particles. The variability of [CO<sub>2</sub>(g)] in subsoils should be considered in the P cycle in calcareous soils.</p>","PeriodicalId":101043,"journal":{"name":"Proceedings - Soil Science Society of America","volume":"89 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145101101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Michel Aldrighi, Juliana Domingues Lima, Paulo Ivan Fernandes-Júnior, Enderson Petrônio de Brito Ferreira
Soybeans (Glycine max (L.) Merr.) are mainly grown in Brazil during the rainy season. However, there are typically periods of rainfall deficiency, which causes water-deficit stress to the crop. Plant growth-promoting rhizobacteria (PGPR) can help alleviate these stresses by inducing water deficit tolerance. The objective of this study was to evaluate the role of PGPR in enhancing soybean tolerance to water-deficit stress. Six PGPR isolates, two for induction of water-deficit tolerance (ESA 441, BRM 034008), two AIA-producing (Ab-V5, BRM 063574), and two phosphate solubilizing (BRM 063573, BRM 67205), and their combination were evaluated, for a total of 16 treatments. The experiment was conducted in a greenhouse using a randomized block design with three replicates. Effects were measured on gas exchange parameters (stomatal conductance, transpiration, internal CO2 concentration, and photosynthetic rate), growth parameters (shoot dry weight, root dry weight, root length, root surface area, root diameter, and root volume), and yield components (pod weight, number of pods, number of grains, and grain weight). Co-inoculation significantly reduces the effects of water stress on gas exchange, plant growth, and productivity compared to single inoculation. Notable combinations, such as BRM 063574 + BRM 67205 + BRM 034008 and BRM 063574 + BRM 063573 + ESA 441, improved root and shoot growth under stress conditions. Yield components also improved with co-inoculations, with combinations such as BRM 063574 + BRM 67205 + ESA 441 showing the highest efficacy. These results suggest that specific PGPR co-inoculations can improve soybean resilience to water deficit stress and promote better growth and yield.
{"title":"Performance of soybeans inoculated with multifunctional microorganisms under water-deficit stress","authors":"Michel Aldrighi, Juliana Domingues Lima, Paulo Ivan Fernandes-Júnior, Enderson Petrônio de Brito Ferreira","doi":"10.1002/saj2.70131","DOIUrl":"10.1002/saj2.70131","url":null,"abstract":"<p>Soybeans (<i>Glycine max</i> (L.) Merr.) are mainly grown in Brazil during the rainy season. However, there are typically periods of rainfall deficiency, which causes water-deficit stress to the crop. Plant growth-promoting rhizobacteria (PGPR) can help alleviate these stresses by inducing water deficit tolerance. The objective of this study was to evaluate the role of PGPR in enhancing soybean tolerance to water-deficit stress. Six PGPR isolates, two for induction of water-deficit tolerance (ESA 441, BRM 034008), two AIA-producing (Ab-V5, BRM 063574), and two phosphate solubilizing (BRM 063573, BRM 67205), and their combination were evaluated, for a total of 16 treatments. The experiment was conducted in a greenhouse using a randomized block design with three replicates. Effects were measured on gas exchange parameters (stomatal conductance, transpiration, internal CO<sub>2</sub> concentration, and photosynthetic rate), growth parameters (shoot dry weight, root dry weight, root length, root surface area, root diameter, and root volume), and yield components (pod weight, number of pods, number of grains, and grain weight). Co-inoculation significantly reduces the effects of water stress on gas exchange, plant growth, and productivity compared to single inoculation. Notable combinations, such as BRM 063574 + BRM 67205 + BRM 034008 and BRM 063574 + BRM 063573 + ESA 441, improved root and shoot growth under stress conditions. Yield components also improved with co-inoculations, with combinations such as BRM 063574 + BRM 67205 + ESA 441 showing the highest efficacy. These results suggest that specific PGPR co-inoculations can improve soybean resilience to water deficit stress and promote better growth and yield.</p>","PeriodicalId":101043,"journal":{"name":"Proceedings - Soil Science Society of America","volume":"89 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://acsess.onlinelibrary.wiley.com/doi/epdf/10.1002/saj2.70131","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145022055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mathematical models are used extensively to estimate soil pesticide leaching in regulatory risk assessments and are often solved numerically, which can obscure simple insights. We developed an analytical solution that highlights the role of the ratio of sorption to degradation in compound leaching, denoted as the sorption-extinction (Se) coefficient. We extend the classic analytical work of Jury to derive a steady-state solution for pesticide concentrations as a function of soil depth considering nonlinear sorption. We consider degradation in the soil water and solid phases and transport driven by advection, diffusion, and dispersion. Nonlinear sorption was handled using the mathematical technique of asymptotic expansions. We compared the steady-state analytic solution with extended duration simulations of the European regulatory numerical model PEARL for all FOCUS scenarios (i.e., nine European regions). The analytic solution was consistent with the long-term PEARL results across most FOCUS scenarios, and the results show that for a fixed Se coefficient, similar mean pesticide concentrations at the regulatory leaching depth (1 m) are obtained despite varying the sorption and degradation by an order of magnitude. This indicates that the Se coefficient is a dominant component of mean leaching behavior rather than degradation or sorption alone. However, as the absolute value of degradation and sorption decreases, variability of the pesticide concentration increases. While we demonstrate the approach using the FOCUS scenarios weather and soil data, this method can be applied as a rapid and time-efficient predictive tool for any region with either highly or more scarcely parameterized soil/weather data.
{"title":"Analytical equation for rapid estimation of pesticide leaching risk accounting for nonlinear sorption with bulk soil biodegradation","authors":"S. Ruiz, S. Payvandi, P. Sweeney, T. Roose","doi":"10.1002/saj2.70120","DOIUrl":"10.1002/saj2.70120","url":null,"abstract":"<p>Mathematical models are used extensively to estimate soil pesticide leaching in regulatory risk assessments and are often solved numerically, which can obscure simple insights. We developed an analytical solution that highlights the role of the ratio of sorption to degradation in compound leaching, denoted as the sorption-extinction (<i>S<sub>e</sub></i>) coefficient. We extend the classic analytical work of Jury to derive a steady-state solution for pesticide concentrations as a function of soil depth considering nonlinear sorption. We consider degradation in the soil water and solid phases and transport driven by advection, diffusion, and dispersion. Nonlinear sorption was handled using the mathematical technique of asymptotic expansions. We compared the steady-state analytic solution with extended duration simulations of the European regulatory numerical model PEARL for all FOCUS scenarios (i.e., nine European regions). The analytic solution was consistent with the long-term PEARL results across most FOCUS scenarios, and the results show that for a fixed <i>S<sub>e</sub></i> coefficient, similar mean pesticide concentrations at the regulatory leaching depth (1 m) are obtained despite varying the sorption and degradation by an order of magnitude. This indicates that the <i>S<sub>e</sub></i> coefficient is a dominant component of mean leaching behavior rather than degradation or sorption alone. However, as the absolute value of degradation and sorption decreases, variability of the pesticide concentration increases. While we demonstrate the approach using the FOCUS scenarios weather and soil data, this method can be applied as a rapid and time-efficient predictive tool for any region with either highly or more scarcely parameterized soil/weather data.</p>","PeriodicalId":101043,"journal":{"name":"Proceedings - Soil Science Society of America","volume":"89 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://acsess.onlinelibrary.wiley.com/doi/epdf/10.1002/saj2.70120","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144998690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}