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Water absorption from air and ammonia loss from urea fertilizers applied to grassland in southeastern United States 美国东南部草地施用尿素肥料的空气吸水率和氨流失量
Pub Date : 2024-10-17 DOI: 10.1002/saj2.20778
Miguel L. Cabrera, Dorcas Franklin, David Kissel

Information on water absorption from the air by urea fertilizers and on NH3 loss when applied to grasslands is limited. Urea application to grassland is typically broadcast (Bcast), whereas urea-ammonium nitrate (UAN) is applied either Bcast or in bands (dribble). This work was conducted to (1) evaluate water absorption from the air by Bcast granular urea, Bcast UAN, and dribble UAN under laboratory conditions, and (2) compare NH3 losses from Bcast urea, Bcast UAN, and dribble UAN when applied to a grassland. Six field studies were conducted from 2017 to 2019. In the laboratory, Bcast UAN exposed to 100% relative humidity absorbed water from air at a faster rate than dribble UAN and Bcast urea. In the field, all three fertilizers lost similar amounts of NH3 when applied to relatively wet soil (> −0.1 MPa). In contrast, when the fertilizers were applied to dry soil (≤ −1.2 MPa), Bcast UAN lost the most NH3 (17.3% and 19.8%) likely because of its capacity to absorb water from the air. Also, at −1.2 MPa, dribble UAN lost more NH3 than Bcast urea (15.3 vs. 10.7%, p < 0.05), probably because the low osmotic potential of UAN (−55 MPa) allowed it to absorb water from the soil at a faster rate than urea could absorb water from the air. In contrast, when the soil water potential was −5.7 MPa, dribble UAN lost less NH3 than Bcast urea (4.4 vs. 17.3%, p < 0.05), likely because the low soil water potential reduced its water absorption.

有关尿素肥料从空气中吸收水分以及施用到草地上的 NH3 损失的信息非常有限。在草地上施用尿素通常采用播撒(Bcast)方式,而硝酸尿素铵(UAN)则采用播撒或带状(滴灌)施用方式。这项工作的目的是:(1) 评估在实验室条件下播撒颗粒尿素、播撒尿素-硝酸铵和点播尿素-硝酸铵从空气中吸收水分的情况;(2) 比较在草地上施用播撒尿素、播撒尿素-硝酸铵和点播尿素-硝酸铵时的 NH3 损失情况。从 2017 年到 2019 年,共进行了六次实地研究。在实验室中,暴露在 100%相对湿度下的 Bcast UAN 从空气中吸收水分的速度快于 Dribble UAN 和 Bcast 尿素。在田间,当施用到相对潮湿的土壤(> -0.1兆帕)中时,这三种肥料损失的 NH3 量相似。相反,在干燥土壤(≤-1.2 兆帕)中施用这些肥料时,Bcast UAN 的 NH3 损失量最大(17.3% 和 19.8%),这可能是因为它具有从空气中吸收水分的能力。此外,在-1.2 兆帕时,滴灌尿素比加注尿素损失更多的 NH3(15.3% 对 10.7%,p <0.05),这可能是因为 UAN 的低渗透势(-55 兆帕)使其从土壤中吸收水分的速度快于尿素从空气中吸收水分的速度。相比之下,当土壤水势为 -5.7 MPa 时,滴灌尿素的 NH3 损失少于滴播尿素(4.4% vs. 17.3%,p < 0.05),这可能是因为土壤水势低降低了尿素的吸水性。
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引用次数: 0
Effects of maize residue and biochar applications on soil δ13C and organic carbon sources in a subtropical paddy rice ecosystem 施用玉米秸秆和生物炭对亚热带水稻生态系统中土壤 δ13C 和有机碳源的影响
Pub Date : 2024-10-16 DOI: 10.1002/saj2.20773
Qiang Jin, Weiqi Wang, Xuyang Liu, Shaoying Lin, Jordi Sardans, Yunying Fang, Tony Vancov, Akash Tariq, Fanjiang Zeng, Josep Peñuelas

This study investigates the utility of plant δ¹3C natural labeling in predicting the impacts of environmental shifts on carbon cycling within ecosystems, particularly focusing on paddy fields treated with maize (Zea mays L.) residues and biochar. Specifically, it examines how soil δ¹3C and the sources of soil organic carbon (SOC), respond in paddy fields (which cultivate C3 plants like rice) when amended with maize residues, maize biochar, and silica-enriched biochar (derived from C4 plants). Conducted in the Fuzhou paddy fields, the experiment included control groups and treatment groups with maize residue (4 t ha⁻¹), maize biochar (4 t ha⁻¹), and silicon-modified maize biochar (4 t ha⁻¹) during both the early and late rice growth periods. The results indicate that all soil treatments increased soil δ¹3C. The application of maize residues notably affected the δ¹3C of the upper soil profile (0–15 cm) differently from the deeper layers (15–30 cm), and it increased soil organic C more than biochar or silicon-modified maize biochar. Soil available P (AP) and pH emerged as significant factors linking δ¹3C, influencing rice yield through changes in soil physicochemical properties. Unlike maize residues, which reduced rice yields, applications of biochar and silicon-modified maize biochar increased rice yields. The latter, which was particularly effective in lowering SOC decomposition rates and addressing rice's silica needs, emerged as the preferred option. The study highlights maize biochar and silicon-modified maize biochar as sustainable alternatives to maize residues for rice cultivation, enhancing soil fertility, carbon pool stability, and yields.

本研究调查了植物δ¹3C 天然标记在预测环境变化对生态系统内碳循环的影响方面的实用性,尤其侧重于使用玉米(Zea mays L.)残留物和生物炭处理过的水稻田。具体而言,该研究考察了水稻田(种植水稻等 C3 植物)中的土壤δ¹3C 和土壤有机碳(SOC)来源在添加玉米残留物、玉米生物炭和富硅生物炭(从 C4 植物中提取)后的反应。实验在福州水田进行,包括对照组和在水稻生长早期和晚期使用玉米残渣(4 吨/公顷-¹)、玉米生物炭(4 吨/公顷-¹)和硅改性玉米生物炭(4 吨/公顷-¹)的处理组。结果表明,所有土壤处理方法都增加了土壤中的δ¹3C。施用玉米秸秆对上层土壤剖面(0-15 厘米)和深层土壤剖面(15-30 厘米)δ¹3C 的影响明显不同,玉米秸秆比生物炭或硅改性玉米生物炭更能增加土壤有机碳。土壤可利用磷(AP)和 pH 值是连接δ¹3C 的重要因素,通过改变土壤理化性质影响水稻产量。玉米残留物会降低水稻产量,而施用生物炭和硅改性玉米生物炭则会提高水稻产量。后者在降低 SOC 分解率和满足水稻对硅的需求方面尤为有效,因此成为首选方案。该研究强调,玉米生物炭和硅改性玉米生物炭可替代玉米残渣用于水稻种植,提高土壤肥力、碳池稳定性和产量。
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引用次数: 0
Toward a community-engaged framework for urban soil research 建立社区参与的城市土壤研究框架
Pub Date : 2024-10-16 DOI: 10.1002/saj2.20776
Tiffany A. Legg, Caitlin Hodges

Researchers in urban environments sample where people live and work. However, there is limited extant guidance available to scientists engaging with community stakeholders to sample soils in urban settings. Leveraging our cumulative experiences, insights gained from community collaborations, and interdisciplinary literature, we present a community-engaged framework for urban soils research. Community-engaged research frameworks emerged over the past two decades to foster trust and respect between communities and researchers as a response to historical exploitation of communities by the academy. Today, these frameworks have become standard for social and public health researchers investigating the physical well-being of communities. However, there is no equivalent framework for scientists studying the soils that underpin the physical and ecological well-being of the same communities. Here, we present the first such framework for soil scientists that incorporates nuanced aspects that are often overlooked. Our proposed framework recognizes the iterative nature of collaboration with community stakeholders and highlights the significance of ethical considerations throughout the research process by emphasizing protection of community stakeholders from harm, involvement of all parties in decision-making processes, maintaining informed consent, and fostering mutual accountability among researchers throughout the research and sampling process.

城市环境中的研究人员会在人们生活和工作的地方取样。然而,对于科学家与社区利益相关者合作在城市环境中进行土壤采样,现有的指导非常有限。利用我们积累的经验、从社区合作中获得的见解以及跨学科文献,我们提出了城市土壤研究的社区参与框架。社区参与式研究框架出现于过去二十年,旨在促进社区与研究人员之间的信任和尊重,以应对学术界对社区的历史性剥削。如今,这些框架已成为社会和公共卫生研究人员调查社区物质福利的标准。然而,对于研究支撑同一社区物质和生态福祉的土壤的科学家来说,还没有相应的框架。在此,我们为土壤科学家提出了第一个这样的框架,其中包含了经常被忽视的细微方面。我们提出的框架认识到了与社区利益相关者合作的反复性,并通过强调保护社区利益相关者不受伤害、让各方参与决策过程、保持知情同意以及在整个研究和取样过程中促进研究人员之间的相互问责,突出了在整个研究过程中考虑伦理因素的重要性。
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引用次数: 0
Calibration of TEROS 10 and TEROS 12 electromagnetic soil moisture sensors 校准 TEROS 10 和 TEROS 12 电磁土壤水分传感器
Pub Date : 2024-10-15 DOI: 10.1002/saj2.20777
Sofia Cominelli, Leonardo D. Rivera, William G. Brown, Tyson E. Ochsner, Andres Patrignani

The TEROS 10 and TEROS 12 are widely used capacitance-based sensors for measuring volumetric water content (θv${{theta }_v}$), but few systematic studies of the measurement volume and accuracy of these sensors have been published. The objectives of this study were to (1) calibrate and validate these sensors in mineral soils, (2) determine the sensing volume of each sensor, and (3) evaluate the temperature sensitivity of the sensors. Both sensors were calibrated in the laboratory using columns of packed soil at multiple moisture levels from air-dry to near saturation. Sensors were validated using additional laboratory and field measurements. The sensing volume was determined by quantifying the response of raw sensor outputs while increasing the level of oven-dry sand, moist sand (0.100 cm3 cm−3), or water around the sensor in the radial and axial directions. Temperature sensitivity was tested in controlled conditions over a range of temperatures from 2°C to 40°C using encapsulated sensors in packed soil columns at constant θv${{theta }_{v}}$. Linear calibration models resulted in mean absolute error ≤0.030 cm3 cm−3 for both sensors during laboratory and field validations. In moist sand, the sensing volume contributing 95% of the maximum sensor response was 439 cm3 for the TEROS 10 and 423 cm3 for the TEROS 12. Both sensors exhibited changes in θv${{theta }_v}$ of ≤0.02 cm3 cm−3 when subjected to a temperature change from 2°C to 40°C. For the soils considered here, both sensors demonstrated accuracy that is likely sufficient for a variety of agricultural and hydrological applications.

TEROS 10 和 TEROS 12 是广泛使用的基于电容的传感器,用于测量体积含水量(θ v ${{theta }_v}$),但有关这些传感器的测量体积和精度的系统研究却鲜有发表。本研究的目标是:(1) 在矿质土壤中校准和验证这些传感器;(2) 确定每个传感器的感应体积;(3) 评估传感器的温度灵敏度。这两种传感器都是在实验室中使用从风干到接近饱和等多个湿度水平的包装土柱进行校准的。此外,还在实验室和实地测量中对传感器进行了验证。在径向和轴向增加传感器周围的干砂、湿砂(0.100 cm3 cm-3)或水时,通过量化传感器原始输出的响应来确定传感量。在恒定 θ v ${{theta }_{v}}$ 的条件下,使用包装土柱中的封装传感器,在 2°C 至 40°C 的温度范围内,在受控条件下测试了温度灵敏度。在实验室和现场验证过程中,线性校准模型导致两种传感器的平均绝对误差均小于 0.030 cm3 cm-3。在潮湿的沙子中,TEROS 10 传感器最大响应 95% 的感应体积为 439 立方厘米,TEROS 12 传感器为 423 立方厘米。当温度从 2°C 变化到 40°C 时,两个传感器的 θ v ${{theta }_v}$ 变化均小于 0.02 cm3 cm-3。对于本文所考虑的土壤,这两种传感器都显示出足够的精度,可以满足各种农业和水文应用的需要。
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引用次数: 0
Nitrogen source regulates soil nitrification and nitrogen losses more than nitrification inhibitor and herbicide: A laboratory evaluation 氮源比硝化抑制剂和除草剂更能调节土壤硝化和氮损失:实验室评估
Pub Date : 2024-10-10 DOI: 10.1002/saj2.20774
William Neels, Amit Jhala, Virginia Jin, Swetabh Patel, Bijesh Maharjan, Javed Iqbal

Increased environmental nitrogen (N) losses have prompted the use of enhanced efficiency nitrogen fertilizers, including nitrification inhibitors. However, the comparative effects of nitrification inhibitors with conventional nitrogen fertilizers and herbicides on soil nitrification and nitrogen losses remain poorly understood. This study evaluated the impact of a nitrification inhibitor (Instinct NXTGEN), two nitrogen sources (broadcast urea vs. injected aqueous ammonia), and a preemergence herbicide (Acuron) on (1) soil nitrification through a 25-day soil incubation experiment and (2) NH3 volatilization, NO3-N leaching, and N2O-N emissions through a 31-day soil column study in loamy sand soil. In both experiments, treatments included combinations of nitrification inhibitor versus no inhibitor, two nitrogen sources, and preemergence herbicide versus no herbicide. Results revealed that nitrogen source significantly influenced nitrification, with injected aqueous ammonia reducing nitrification by 33% compared to surface broadcast urea. Nitrification inhibitors and herbicide had no effect on soil nitrification. Injected aqueous ammonia reduced NH3 volatilization by 87% compared to surface broadcast urea, but the effect of the nitrification inhibitor on NH3 volatilization was inconsistent across both nitrogen sources. Injected aqueous ammonia led to 39% higher cumulative nitrogen (NO3-N + NH4-N) leaching than urea, while the nitrification inhibitor had an inconsistent effect on NO3-N leaching across both nitrogen sources. No significant differences in N2O-N emissions were observed among treatments, and the herbicide had no effect on any measured parameters. These findings suggest that nitrogen source plays a more critical role in regulating soil nitrogen losses than nitrification inhibitors or herbicides.

环境中氮(N)损失的增加促使人们使用包括硝化抑制剂在内的高效氮肥。然而,人们对硝化抑制剂与传统氮肥和除草剂在土壤硝化和氮损失方面的比较效果仍然知之甚少。本研究通过为期 25 天的土壤培养实验,评估了硝化抑制剂(Instinct NXTGEN)、两种氮源(播撒尿素与注射氨水)和萌芽前除草剂(Acuron)对(1)土壤硝化的影响;以及(2)通过在壤土中进行为期 31 天的土壤柱研究,评估了 NH3 挥发、NO3-N 沥滤和 N2O-N 排放的影响。在这两项实验中,处理包括硝化抑制剂与无抑制剂、两种氮源以及萌芽前除草剂与无除草剂的组合。结果表明,氮源对硝化作用有显著影响,与地表撒施尿素相比,注射氨水可使硝化作用减少 33%。硝化抑制剂和除草剂对土壤硝化没有影响。与地表撒施尿素相比,喷射水氨减少了 87% 的 NH3 挥发,但硝化抑制剂对 NH3 挥发的影响在两种氮源中并不一致。与尿素相比,注入水氨导致的累积氮(NO3-N + NH4-N)淋失量高出 39%,而硝化抑制剂对两种氮源的 NO3-N 淋失量的影响不一致。不同处理之间的 N2O-N 排放量没有明显差异,除草剂对任何测量参数也没有影响。这些研究结果表明,在调节土壤氮素流失方面,氮源比硝化抑制剂或除草剂发挥着更关键的作用。
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引用次数: 0
Identification of plant soil water and soil aeration corequisites: A management tool 确定植物土壤水分和土壤通气核心条件:管理工具
Pub Date : 2024-10-09 DOI: 10.1002/saj2.20772
Beaulah Pragg, M. M. T. Lakshani, T. K. K. Chamindu Deepagoda, Keith Cameron, Hong Di, Timothy J. Clough, Sam Carrick, Bo Elberling, K. Smits

Plant-available soil-water and adequate soil aeration within the root zone are essential corequisites for successful plant growth. Characterization of these two requirements for plant growth is generally done by independent measurements of water and air phase properties and functions in soil, with limited emphasis on their combined effect. This study investigated soil-water characteristic (SWC) measurements in vadose soil profiles (up to 117-cm depth) in six pasture soils to examine the combined behavior of soil-water and diffusion-controlled aeration within the root zone. The soil moisture measurements were made over matric potentials ranging from −1 to −1500 kPa using tension table and pressure plate apparatus. The van Genuchten model was used to parameterize the measured SWC curve, while the Millington–Quick model was used to derive soil-gas diffusivity from measured soil physical properties. Based on two simple indices, derived to represent plant-available soil-water and soil aeration status at a given moisture content, we propose an assessment matrix, which illustrates how a soil satisfies the corequisites for successful plant growth, as it drains to different matric potentials upon irrigation. Results show a pronounced effect of soil texture and, to a lesser extent, soil structure on satisfying these corequisites. With the aid of the assessment matrix, we observed that loam, sandy loam textures exhibited a good overall performance, while sand, clay loam, and clay soils struggled to meet these corequisites during drainage.

植物根区可利用的土壤水和充分的土壤通气是植物成功生长的必要核心条件。对植物生长的这两项要求的特征描述通常是通过对土壤中水相和气相的特性和功能进行独立测量来完成的,而对它们的综合效应的强调却很有限。本研究调查了六种牧场土壤中伏流土壤剖面(最深 117 厘米)的土壤水分特征(SWC)测量结果,以研究根区土壤水分和扩散控制曝气的综合行为。土壤水分测量是使用拉力台和压力板仪器在-1 到 -1500 kPa 的母质电位范围内进行的。Van Genuchten 模型用于对测量的 SWC 曲线进行参数化,而 MillingtonQuick 模型则用于从测量的土壤物理特性推导出土壤-气体扩散率。根据两个简单的指数,我们提出了一个评估矩阵,这两个指数代表了在给定含水量下植物可利用的土壤水分和土壤通气状况,说明了土壤在灌溉后排水到不同的熟化电位时,如何满足植物成功生长的核心条件。结果表明,土壤质地对满足这些核心条件有明显影响,其次是土壤结构。借助评估矩阵,我们观察到壤土、沙壤土的质地表现出良好的整体性能,而沙土、粘壤土和粘土在排水过程中难以满足这些核心条件。
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引用次数: 0
Elimination of microplastic from soil by pyrolysis and potential hazard analysis: Effects of pyrolysis temperature and holding time 通过热解消除土壤中的微塑料及潜在危害分析:热解温度和保温时间的影响
Pub Date : 2024-10-06 DOI: 10.1002/saj2.20770
Kaiying Qiu, Lingling Zhang, Shibing Xie, Mengwei Zheng, Zhenyu Wang, Zefeng Wang

Efficient and harmless treatment of microplastics in soil is the current focus of microplastic pollution management. In this study, the pyrolysis effect of soil microplastics at different temperatures and holding times was analyzed. The results showed that temperature and holding time significantly affected the morphology and compositional structure of soil. Pure microplastics and product adhesion after pyrolysis at low temperature (400°C) were not easy to remove. Interestingly, compared with pure polyethylene (PE), direct pyrolysis of microplastics in soil could obtain better results, and the content of organic matter and polycyclic aromatic hydrocarbons (PAH) could be significantly reduced. However, the pyrolysis performance was very unsatisfactory even if the action time was prolonged at 400°C. Prolonged high-temperature treatment accelerated the fragmentation of the alkane main chain of PE, thus generating more PAH, and the temperature was not high enough (400°C) for further effective degradation of PAH into carbon dioxide and water. Therefore, higher temperature and longer holding time need to be selected to ensure the pyrolysis performance. This study revealed the role and mechanism of pyrolysis in soil microplastic mixed systems, aiming to provide a basis for the treatment of microplastics in terrestrial ecosystems.

高效、无害地处理土壤中的微塑料是当前微塑料污染治理的重点。本研究分析了不同温度和保温时间下土壤微塑料的热解效果。结果表明,温度和保温时间对土壤的形态和成分结构有显著影响。低温(400°C)热解后的纯微塑料和产品附着物不易去除。有趣的是,与纯聚乙烯(PE)相比,直接热解土壤中的微塑料可获得更好的效果,有机物和多环芳烃(PAH)的含量可明显降低。然而,即使在 400°C 下延长作用时间,热解效果也很不理想。长时间的高温处理会加速聚乙烯烷烃主链的破碎,从而产生更多的多环芳烃,而且温度不够高(400°C),无法进一步有效地将多环芳烃降解为二氧化碳和水。因此,需要选择更高的温度和更长的保温时间,以确保热解性能。本研究揭示了热解在土壤微塑料混合系统中的作用和机理,旨在为陆地生态系统中微塑料的处理提供依据。
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引用次数: 0
An updated method for identifying the formative factors in soil structure 确定土壤结构形成因素的最新方法
Pub Date : 2024-10-06 DOI: 10.1002/saj2.20771
Amber D. Anderson, C. Lee Burras, Judith Turk, Rebecca Young, Nic Jelinski, Colby Moorberg, DeAnn Presley, Kris Osterloh, Ashlee Dere, Kerry Clark

Soil structure is a standard component of pedon descriptions collected to characterize the nature and function of soils as a natural body. Soil structure is an important indicator of soil development and a predictor of water, air, and root movement throughout the profile. However, inconsistencies exist within the current standardized methodologies used to describe soil structure in the field, making soil structure data difficult to use and often confusing to students encountering these concepts for the first time. Therefore, we propose an updated method of soil structure description to add an interpretive component that clearly distinguishes geogenic, pedogenic, and human-induced structures. This method employs terminology that communicates both observable morphology and the mechanism of formation. The updated method herein differentiates three classes of structure: (a) geogenic structural formation (GS), (b) natural pedological structural formation (current nomenclature), and (c) significant human-induced structure changes from natural pedological structure. This updated approach improves the documentation of scientifically relevant soil morphology and communicates the processes involved in the formation of all forms of soil structure. As a result, this change eases description challenges around soil structure and interpretations common in soil science classes, including soil judging.

土壤结构是为描述土壤作为自然体的性质和功能而收集的血统描述的标准组成部分。土壤结构是土壤发育的重要指标,也是整个剖面中水、空气和根系运动的预测指标。然而,目前用于实地描述土壤结构的标准化方法存在不一致的地方,使得土壤结构数据难以使用,对于初次接触这些概念的学生来说也常常感到困惑。因此,我们提出了一种最新的土壤结构描述方法,增加了解释性内容,明确区分了地质结构、成土结构和人为结构。这种方法采用的术语既能表达可观察到的形态,又能表达形成机制。更新后的方法将结构分为三类:(a) 地质结构形成(GS),(b) 天然成土结构形成(当前术语),(c) 由人类引起的天然成土结构的重大变化。这一更新方法改进了与科学相关的土壤形态记录,并传达了所有土壤结构形式的形成过程。因此,这一变化缓解了土壤学课程(包括土壤判断)中常见的有关土壤结构和解释的描述难题。
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引用次数: 0
Spectral signatures of soil horizons and soil orders from Wisconsin 威斯康星州土壤层和土壤等级的光谱特征
Pub Date : 2024-09-29 DOI: 10.1002/saj2.20766
Malithi Weerasekara, Alfred E. Hartemink, Yakun Zhang, Annalisa Stevenson

We used mid-infrared (MIR) spectra (4000–600 cm−1) to identify and classify soil orders and soil horizons from 102 pedons across five soil orders (Alfisols, Entisols, Mollisols, Spodosols, and Histosols). The soils were analyzed for texture, total carbon, pH, and elemental properties. Random forest models were used to group the spectra of master horizons (O, A, E, B, and C), B horizons (Bs, Bt, and Bw), and the five soil orders. The prediction accuracies for the master horizons and B horizons were 0.81 and 0.89, respectively. The Kappa coefficient was 0.71 for the prediction of master horizons and 0.73 for the prediction of B horizons. The soil orders had an overall accuracy of 0.73 and a Kappa coefficient of 0.64. Histosols exhibited unique absorption characteristics at 2930 and 2860 cm−1 that differed distinctly from mineral soils. The MIR spectra accurately distinguished the O horizons. The spectral curve of topsoil of Spodosols was comparable to the O horizons. Spodosols under forest had A horizons with high organic matter and were classified accurately. Entisols (Psamments) displayed absorption peaks associated with sand, facilitating their differentiation from the other soil orders. The model struggled to discern subtle differences among some soil orders, and identification is hampered if soils undergo irreversible changes upon drying. However, our results showed that MIR spectra can be used for effectively identifying and classifying soil orders as well as soil horizons.

我们利用中红外(MIR)光谱(4000-600 cm-1)对五种土壤类型(Alfisols、Entisols、Mollisols、Spodosols 和 Histosols)102 块土壤的土壤类型和土壤层进行了识别和分类。对土壤的质地、总碳、pH 值和元素特性进行了分析。使用随机森林模型对主地层(O、A、E、B 和 C)、B 地层(Bs、Bt 和 Bw)以及五个土壤等级的光谱进行分组。主层和 B 层的预测准确度分别为 0.81 和 0.89。主层预测的 Kappa 系数为 0.71,B 层预测的 Kappa 系数为 0.73。土壤阶次的总体准确度为 0.73,Kappa 系数为 0.64。组织溶胶在 2930 和 2860 cm-1 处表现出与矿质土壤截然不同的独特吸收特征。近红外光谱可准确区分 O 层。Spodosols 表层土的光谱曲线与 O 层相当。森林下的 Spodosols 具有高有机质的 A 层,分类准确。Entisols(Psamments)显示出与沙有关的吸收峰,有助于将其与其他土壤等级区分开来。该模型难以辨别某些土壤类别之间的细微差别,如果土壤在干燥后发生不可逆的变化,则会影响识别。不过,我们的研究结果表明,近红外光谱可用于有效识别和划分土壤类别以及土壤层。
{"title":"Spectral signatures of soil horizons and soil orders from Wisconsin","authors":"Malithi Weerasekara,&nbsp;Alfred E. Hartemink,&nbsp;Yakun Zhang,&nbsp;Annalisa Stevenson","doi":"10.1002/saj2.20766","DOIUrl":"https://doi.org/10.1002/saj2.20766","url":null,"abstract":"<p>We used mid-infrared (MIR) spectra (4000–600 cm<sup>−1</sup>) to identify and classify soil orders and soil horizons from 102 pedons across five soil orders (Alfisols, Entisols, Mollisols, Spodosols, and Histosols). The soils were analyzed for texture, total carbon, pH, and elemental properties. Random forest models were used to group the spectra of master horizons (O, A, E, B, and C), B horizons (Bs, Bt, and Bw), and the five soil orders. The prediction accuracies for the master horizons and B horizons were 0.81 and 0.89, respectively. The Kappa coefficient was 0.71 for the prediction of master horizons and 0.73 for the prediction of B horizons. The soil orders had an overall accuracy of 0.73 and a Kappa coefficient of 0.64. Histosols exhibited unique absorption characteristics at 2930 and 2860 cm<sup>−1</sup> that differed distinctly from mineral soils. The MIR spectra accurately distinguished the O horizons. The spectral curve of topsoil of Spodosols was comparable to the O horizons. Spodosols under forest had A horizons with high organic matter and were classified accurately. Entisols (Psamments) displayed absorption peaks associated with sand, facilitating their differentiation from the other soil orders. The model struggled to discern subtle differences among some soil orders, and identification is hampered if soils undergo irreversible changes upon drying. However, our results showed that MIR spectra can be used for effectively identifying and classifying soil orders as well as soil horizons.</p>","PeriodicalId":101043,"journal":{"name":"Proceedings - Soil Science Society of America","volume":"88 6","pages":"2013-2030"},"PeriodicalIF":0.0,"publicationDate":"2024-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/saj2.20766","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142642486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Bacillus siamensis orchestrates plant gene reprogramming and rhizosphere microbiome reshaping to bolster maize crop performance 暹罗芽孢杆菌协调植物基因重编程和根瘤微生物组重塑,提高玉米作物产量
Pub Date : 2024-09-27 DOI: 10.1002/saj2.20768
Bin Sun, Chenyu Sun, Wenjiang Fu, Huijing Fu, Xiaolong Shu, Mengfan Wu, Qiao Guo, Hangxian Lai

Plant growth-promoting rhizobacteria (e.g., Bacillus) confer health benefits to the host. Bacillus siamensis has been used to control plant disease in fruits and vegetables, but its potential effects on crop performance remain unclear. This study investigated changes in the growth, root transcriptomic profile, and rhizosp here microbiome of maize plants (Zea may L.) following inoculation with B. siamensis 33. All inoculated plants grew better than non-inoculated controls in pots, as exemplified by 24.0% and 6.8% increase in plant height and stem diameter, respectively (< 0.01). Shoot dry weight also increased by 20.6% upon inoculation, accompanied by 46.9% increase in root dry weight (< 0.01). Transcriptomic analysis revealed that inoculation induced (2.16- to 5.53-fold) upregulated expression of genes related to auxin signal transduction in maize. The expression of genes involved in phenylpropanoid biosynthesis, glutathione metabolism, and plant defense-related signal (e.g., jasmonic acid and salicylic acid) transduction was (2.06- to 11.30-fold) upregulated in inoculated plants. Upon inoculation, bacterial α-diversity trended higher and potentially beneficial taxa (e.g., Sphingobium, Flavihumibacter, and Parasegetibacter) were enriched in the rhizosphere, likely a result of enhanced root exudation of specific metabolites (e.g., flavonoids) and subsequent recruitment of beneficial microbial taxa. Potential microbial functions associated with nitrogen cycling and pollutant degradation were stimulated by inoculation. These findings indicate that B. siamensis 33 mediates the reprogramming of plant gene expression and reshaping of rhizosphere microbiome structure to bolster maize crop performance.

植物生长促进根瘤菌(如芽孢杆菌)对宿主的健康有益。暹罗芽孢杆菌已被用于控制水果和蔬菜中的植物病害,但其对作物生长的潜在影响仍不清楚。本研究调查了玉米植物(Zea may L.)接种 B. siamensis 33 后生长、根部转录组概况和根瘤微生物组的变化。在盆栽中,所有接种的植株都比未接种的对照组生长得更好,植株高度和茎秆直径分别增加了 24.0% 和 6.8%(p <0.01)。接种后,芽干重也增加了 20.6%,根干重增加了 46.9%(p <0.01)。转录组分析表明,接种诱导(2.16-5.53 倍)玉米中与辅助素信号转导相关的基因表达上调。参与苯丙酮生物合成、谷胱甘肽代谢和植物防御相关信号(如茉莉酸和水杨酸)转导的基因在接种植株中的表达上调(2.06-11.30 倍)。接种后,细菌的α-多样性呈上升趋势,根圈中潜在的有益类群(如Sphingobium、Flavihumibacter和Parasegetibacter)富集,这可能是特定代谢产物(如类黄酮)的根渗出增强以及随后有益微生物类群招募的结果。接种后,与氮循环和污染物降解相关的潜在微生物功能受到刺激。这些研究结果表明,B. siamensis 33介导了植物基因表达的重编程和根瘤微生物群结构的重塑,从而提高了玉米的作物表现。
{"title":"Bacillus siamensis orchestrates plant gene reprogramming and rhizosphere microbiome reshaping to bolster maize crop performance","authors":"Bin Sun,&nbsp;Chenyu Sun,&nbsp;Wenjiang Fu,&nbsp;Huijing Fu,&nbsp;Xiaolong Shu,&nbsp;Mengfan Wu,&nbsp;Qiao Guo,&nbsp;Hangxian Lai","doi":"10.1002/saj2.20768","DOIUrl":"https://doi.org/10.1002/saj2.20768","url":null,"abstract":"<p>Plant growth-promoting rhizobacteria (e.g., <i>Bacillus</i>) confer health benefits to the host. <i>Bacillus siamensis</i> has been used to control plant disease in fruits and vegetables, but its potential effects on crop performance remain unclear. This study investigated changes in the growth, root transcriptomic profile, and rhizosp here microbiome of maize plants (<i>Zea may</i> L.) following inoculation with <i>B. siamensis</i> 33. All inoculated plants grew better than non-inoculated controls in pots, as exemplified by 24.0% and 6.8% increase in plant height and stem diameter, respectively (<i>p </i>&lt; 0.01). Shoot dry weight also increased by 20.6% upon inoculation, accompanied by 46.9% increase in root dry weight (<i>p </i>&lt; 0.01). Transcriptomic analysis revealed that inoculation induced (2.16- to 5.53-fold) upregulated expression of genes related to auxin signal transduction in maize. The expression of genes involved in phenylpropanoid biosynthesis, glutathione metabolism, and plant defense-related signal (e.g., jasmonic acid and salicylic acid) transduction was (2.06- to 11.30-fold) upregulated in inoculated plants. Upon inoculation, bacterial α-diversity trended higher and potentially beneficial taxa (e.g., <i>Sphingobium</i>, <i>Flavihumibacter</i>, and <i>Parasegetibacter</i>) were enriched in the rhizosphere, likely a result of enhanced root exudation of specific metabolites (e.g., flavonoids) and subsequent recruitment of beneficial microbial taxa. Potential microbial functions associated with nitrogen cycling and pollutant degradation were stimulated by inoculation. These findings indicate that <i>B. siamensis</i> 33 mediates the reprogramming of plant gene expression and reshaping of rhizosphere microbiome structure to bolster maize crop performance.</p>","PeriodicalId":101043,"journal":{"name":"Proceedings - Soil Science Society of America","volume":"88 6","pages":"2031-2045"},"PeriodicalIF":0.0,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142642518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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Proceedings - Soil Science Society of America
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