Susanna Miescher, Florine Schleiffer, Eliane Wegenstein and Selçuk Yildirim
The growing demand for sustainable materials has intensified interest in bio-based and biodegradable polymers as alternatives to fossil-based plastics. This study investigated the development of injection-molded biocomposites based on poly(butylene succinate) (PBS), poly(butylene adipate-co-terephthalate) (PBAT), and their blends, reinforced with 30–70 wt% potato peels (PP), an abundant by-product of food processing. The effects of filler content and polymer composition on thermal, mechanical, and moisture-related properties were systematically evaluated. All composites remained thermally stable below 228 °C, confirming the suitability of PP for melt processing. FTIR spectroscopy showed no evidence of chemical bonding between filler and polymer matrices, although weak physical interactions were observed, particularly in PBS-rich systems. In contrast, blending PBS with PBAT indicated polymer–polymer interactions, suggesting partial compatibilization, as reflected in a 1.8-fold increase in elongation at break. PP addition consistently altered composite structure and significantly enhanced stiffness, with the elastic modulus increasing from 698 to 1825 MPa for PBS (+162%) and from 77 to 1161 MPa for PBAT (+1418%) at 70 wt% PP. Conversely, tensile strength decreased from 35.0 to 10.6 MPa (PBS) and from 17.1 to 6.5 MPa (PBAT), and elongation at break dropped below 3% for all composites containing ≥40 wt% PP. Overall, PBS/PBAT-potato peel composites exhibited more balanced mechanical performance compared to neat PBS or PBAT composites. DSC analysis revealed that PP acted as a nucleating agent in PBS and PBS-rich blends, increasing crystallization temperature with only minor impact on overall crystallinity. Collectively, these findings demonstrate the feasibility of producing high-filler-content biocomposites for sustainable packaging and agricultural materials.
{"title":"Potato peel-based PBS/PBAT biocomposites: influence of composition and filler content on injection molded properties","authors":"Susanna Miescher, Florine Schleiffer, Eliane Wegenstein and Selçuk Yildirim","doi":"10.1039/D5FB00333D","DOIUrl":"https://doi.org/10.1039/D5FB00333D","url":null,"abstract":"<p >The growing demand for sustainable materials has intensified interest in bio-based and biodegradable polymers as alternatives to fossil-based plastics. This study investigated the development of injection-molded biocomposites based on poly(butylene succinate) (PBS), poly(butylene adipate-<em>co</em>-terephthalate) (PBAT), and their blends, reinforced with 30–70 wt% potato peels (PP), an abundant by-product of food processing. The effects of filler content and polymer composition on thermal, mechanical, and moisture-related properties were systematically evaluated. All composites remained thermally stable below 228 °C, confirming the suitability of PP for melt processing. FTIR spectroscopy showed no evidence of chemical bonding between filler and polymer matrices, although weak physical interactions were observed, particularly in PBS-rich systems. In contrast, blending PBS with PBAT indicated polymer–polymer interactions, suggesting partial compatibilization, as reflected in a 1.8-fold increase in elongation at break. PP addition consistently altered composite structure and significantly enhanced stiffness, with the elastic modulus increasing from 698 to 1825 MPa for PBS (+162%) and from 77 to 1161 MPa for PBAT (+1418%) at 70 wt% PP. Conversely, tensile strength decreased from 35.0 to 10.6 MPa (PBS) and from 17.1 to 6.5 MPa (PBAT), and elongation at break dropped below 3% for all composites containing ≥40 wt% PP. Overall, PBS/PBAT-potato peel composites exhibited more balanced mechanical performance compared to neat PBS or PBAT composites. DSC analysis revealed that PP acted as a nucleating agent in PBS and PBS-rich blends, increasing crystallization temperature with only minor impact on overall crystallinity. Collectively, these findings demonstrate the feasibility of producing high-filler-content biocomposites for sustainable packaging and agricultural materials.</p>","PeriodicalId":101198,"journal":{"name":"Sustainable Food Technology","volume":" 6","pages":" 2308-2320"},"PeriodicalIF":0.0,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/fb/d5fb00333d?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145500564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Haida Setyani, Arima Diah Setiowati, Sri Rahayoe, Chusnul Hidayat and Arifin Dwi Saputro
Red palm oil (RPO) is a rich source of carotenoids but is susceptible to degradation and possesses a distinct palm-like odor and taste that may affect the sensory quality of food products. Microencapsulation is considered a potential approach to address these challenges. This study aimed to minimize the aftertaste of RPO through the characterization and formulation of the microencapsulation process. Soy Protein Concentrate (SPC) and carrageenan (CG) were conjugated at various ratios, with the optimal ratio being 3 : 1, which demonstrated improved emulsifying properties, a higher degree of glycation (DG), and greater formation of Maillard reaction products. The resulting microcapsules exhibited a high encapsulation efficiency of up to 82.27% at an oil-to-wall material ratio of 1 : 3, along with good color retention and in situ absorption, as indicated by 82% carotenoid absorption in an in situ intestinal study. The addition of RPO microcapsules to dark chocolate bars did not affect the texture, melting point, morphology, and sensory attributes. However, it had a significant effect (p < 0.05) on color and rheology parameters. Overall, the encapsulation of RPO using the SPC–CG conjugate as the wall material effectively masked the inherent palm-like taste and odor of the oil when incorporated into the chocolate product, making them undetectable to panelists. These findings support the application of protein–polysaccharide conjugates as a wall material for encapsulating bioactive compounds in functional food products.
{"title":"Microencapsulation of red palm oil with soy protein concentrate–carrageenan conjugates for compound dark chocolate applications","authors":"Haida Setyani, Arima Diah Setiowati, Sri Rahayoe, Chusnul Hidayat and Arifin Dwi Saputro","doi":"10.1039/D5FB00278H","DOIUrl":"https://doi.org/10.1039/D5FB00278H","url":null,"abstract":"<p >Red palm oil (RPO) is a rich source of carotenoids but is susceptible to degradation and possesses a distinct palm-like odor and taste that may affect the sensory quality of food products. Microencapsulation is considered a potential approach to address these challenges. This study aimed to minimize the aftertaste of RPO through the characterization and formulation of the microencapsulation process. Soy Protein Concentrate (SPC) and carrageenan (CG) were conjugated at various ratios, with the optimal ratio being 3 : 1, which demonstrated improved emulsifying properties, a higher degree of glycation (DG), and greater formation of Maillard reaction products. The resulting microcapsules exhibited a high encapsulation efficiency of up to 82.27% at an oil-to-wall material ratio of 1 : 3, along with good color retention and <em>in situ</em> absorption, as indicated by 82% carotenoid absorption in an <em>in situ</em> intestinal study. The addition of RPO microcapsules to dark chocolate bars did not affect the texture, melting point, morphology, and sensory attributes. However, it had a significant effect (<em>p</em> < 0.05) on color and rheology parameters. Overall, the encapsulation of RPO using the SPC–CG conjugate as the wall material effectively masked the inherent palm-like taste and odor of the oil when incorporated into the chocolate product, making them undetectable to panelists. These findings support the application of protein–polysaccharide conjugates as a wall material for encapsulating bioactive compounds in functional food products.</p>","PeriodicalId":101198,"journal":{"name":"Sustainable Food Technology","volume":" 6","pages":" 2337-2351"},"PeriodicalIF":0.0,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/fb/d5fb00278h?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145500532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Deepika Shrestha, Alisha Pradhan, V. Sai Sharanya Palletti, R. B. Swethapriya and M. Srijaya
The growing demand for sustainable dairy alternatives has driven innovation in plant-based frozen desserts like oat-based milk substitutes (OMS). In this study, whole oat groats were processed into a plant-based milk substitute using the combined acid–enzyme hydrolysis technique, leveraging their low environmental impact and desirable techno-functional properties, including effective emulsifying and stabilizing capacities to develop basil-flavored oat-based milk substitute ice cream. Among the various formulations explored, OMS replacing dairy milk at 50% and 100% levels, in combination with 10% basil leaf extract, demonstrated superior acceptability and was used for the development of ice cream. The physicochemical, rheological, textural, nutritive, storage, organoleptic, and microbial parameters of the Basil-Flavoured Oat-based Milk Substitute (BF-OMS) ice creams were assessed and compared with full-cream dairy milk (FCDM) ice cream as the control. The experimental samples showed optimum pH and titrable acidity values, i.e., 6.91–6.94 and 0.25–0.27%, respectively. The resultant higher total carbohydrate (40.7–51.76%), total solids (48.70–55.95%), and total ash content (1.05–1.15%) in OMS ice creams led to an increased viscosity of the OMS ice-cream mix, ranging from 35.13 to 146 cP, improving their melting properties and structural integrity. BF-OMS ice creams exhibited pseudoplastic, non-Newtonian behaviour with higher viscosities than the control and a strong power law model fit (R2 = 0.998). The presence of β-glucans in the OMS might have contributed to enhanced gelling and water-binding capacities, resulting in desirable firmness and a smooth texture. The nutritional analysis showed that the partially substituted BF-OMS ice cream maintained the protein content (4.69%), comparable to the control (5.39%). Both 50% and 100% BF-OMS ice-creams had lower fat content (1.1–2.25%) compared to the control (4.1%). Additionally, OMS ice creams exhibited significantly higher total polyphenol content (45.44–46.68 mg GAE/100 g) and DPPH inhibition activity (89.85–92.81%) than the control. More importantly, an increment was observed in total polyphenols (26.34–58.05%) and DPPH inhibition activity (1.70%) in experimental samples at the end of 15 days of storage, indicating enhanced antioxidant potential during storage studies. Overall, the findings suggest that BF-OMS ice creams with dairy milk substitution can support a more sustainable industry transition with high consumer acceptance.
{"title":"Formulation and characterization of basil-flavoured oat-based milk substitute ice cream as a sustainable alternative to dairy ice cream","authors":"Deepika Shrestha, Alisha Pradhan, V. Sai Sharanya Palletti, R. B. Swethapriya and M. Srijaya","doi":"10.1039/D5FB00232J","DOIUrl":"https://doi.org/10.1039/D5FB00232J","url":null,"abstract":"<p >The growing demand for sustainable dairy alternatives has driven innovation in plant-based frozen desserts like oat-based milk substitutes (OMS). In this study, whole oat groats were processed into a plant-based milk substitute using the combined acid–enzyme hydrolysis technique, leveraging their low environmental impact and desirable techno-functional properties, including effective emulsifying and stabilizing capacities to develop basil-flavored oat-based milk substitute ice cream. Among the various formulations explored, OMS replacing dairy milk at 50% and 100% levels, in combination with 10% basil leaf extract, demonstrated superior acceptability and was used for the development of ice cream. The physicochemical, rheological, textural, nutritive, storage, organoleptic, and microbial parameters of the Basil-Flavoured Oat-based Milk Substitute (BF-OMS) ice creams were assessed and compared with full-cream dairy milk (FCDM) ice cream as the control. The experimental samples showed optimum pH and titrable acidity values, <em>i.e.</em>, 6.91–6.94 and 0.25–0.27%, respectively. The resultant higher total carbohydrate (40.7–51.76%), total solids (48.70–55.95%), and total ash content (1.05–1.15%) in OMS ice creams led to an increased viscosity of the OMS ice-cream mix, ranging from 35.13 to 146 cP, improving their melting properties and structural integrity. BF-OMS ice creams exhibited pseudoplastic, non-Newtonian behaviour with higher viscosities than the control and a strong power law model fit (<em>R</em><small><sup>2</sup></small> = 0.998). The presence of β-glucans in the OMS might have contributed to enhanced gelling and water-binding capacities, resulting in desirable firmness and a smooth texture. The nutritional analysis showed that the partially substituted BF-OMS ice cream maintained the protein content (4.69%), comparable to the control (5.39%). Both 50% and 100% BF-OMS ice-creams had lower fat content (1.1–2.25%) compared to the control (4.1%). Additionally, OMS ice creams exhibited significantly higher total polyphenol content (45.44–46.68 mg GAE/100 g) and DPPH inhibition activity (89.85–92.81%) than the control. More importantly, an increment was observed in total polyphenols (26.34–58.05%) and DPPH inhibition activity (1.70%) in experimental samples at the end of 15 days of storage, indicating enhanced antioxidant potential during storage studies. Overall, the findings suggest that BF-OMS ice creams with dairy milk substitution can support a more sustainable industry transition with high consumer acceptance.</p>","PeriodicalId":101198,"journal":{"name":"Sustainable Food Technology","volume":" 6","pages":" 2253-2269"},"PeriodicalIF":0.0,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/fb/d5fb00232j?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145500560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Raj Singh, C. Nickhil, Konga Upendar, Poonam Mishra and Sankar Chandra Deka
The precise prediction of fruit maturity is essential for determining the optimal harvest time. It helps to reduce postharvest losses and maintain consistent fruit quality for consumers. Traditional methods for assessing maturity depend largely on manual inspection. This process is subjective, time-consuming, and prone to human error. Deep learning approaches, particularly convolutional neural networks (CNNs), offer a promising alternative by automating classification with high precision and consistency. This research seeks to identify the most effective deep-learning algorithms for predicting the maturity of mandarin oranges. In this study, the performance of four convolutional neural network architectures (EfficientNet-B0, ResNet50, VGG16, and a Custom CNN) was investigated for the classification of mandarin oranges based on their maturity levels: unripe, ripe, and overripe. The primary dataset comprised 1095 images, with each category containing 365 images. The deep learning models achieved the best accuracy rates of 98% for both EfficientNet-B0 and ResNet50, 83% for VGG16, and an impressive 99% for the Custom CNN, considering primary images. By comparing these models on a balanced dataset, this work offers a practical guide for researchers and practitioners on selecting models for assessing fruit maturity. Notably, EfficientNet-B0, ResNet50, and the Custom CNN exhibited significantly higher success rates compared to VGG16 and existing models, making them particularly recommendable for the development of an efficient automated system for harvesting and sorting mandarin oranges in the near future. The results aim to identify potential applications for improving agricultural practices, quality assessment, and overall efficiency in the food industry.
{"title":"Deep learning-based approach for classifying mandarin orange maturity","authors":"Raj Singh, C. Nickhil, Konga Upendar, Poonam Mishra and Sankar Chandra Deka","doi":"10.1039/D5FB00408J","DOIUrl":"https://doi.org/10.1039/D5FB00408J","url":null,"abstract":"<p >The precise prediction of fruit maturity is essential for determining the optimal harvest time. It helps to reduce postharvest losses and maintain consistent fruit quality for consumers. Traditional methods for assessing maturity depend largely on manual inspection. This process is subjective, time-consuming, and prone to human error. Deep learning approaches, particularly convolutional neural networks (CNNs), offer a promising alternative by automating classification with high precision and consistency. This research seeks to identify the most effective deep-learning algorithms for predicting the maturity of mandarin oranges. In this study, the performance of four convolutional neural network architectures (EfficientNet-B0, ResNet50, VGG16, and a Custom CNN) was investigated for the classification of mandarin oranges based on their maturity levels: unripe, ripe, and overripe. The primary dataset comprised 1095 images, with each category containing 365 images. The deep learning models achieved the best accuracy rates of 98% for both EfficientNet-B0 and ResNet50, 83% for VGG16, and an impressive 99% for the Custom CNN, considering primary images. By comparing these models on a balanced dataset, this work offers a practical guide for researchers and practitioners on selecting models for assessing fruit maturity. Notably, EfficientNet-B0, ResNet50, and the Custom CNN exhibited significantly higher success rates compared to VGG16 and existing models, making them particularly recommendable for the development of an efficient automated system for harvesting and sorting mandarin oranges in the near future. The results aim to identify potential applications for improving agricultural practices, quality assessment, and overall efficiency in the food industry.</p>","PeriodicalId":101198,"journal":{"name":"Sustainable Food Technology","volume":" 6","pages":" 2215-2225"},"PeriodicalIF":0.0,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/fb/d5fb00408j?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145500621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Thi Phuong Linh Le, Jayani Samarathunga, Katrina Strazdins, Jeroen Rens and Benu Adhikari
This study presents a comprehensive physical and functional characterisation of water-soluble protein fractions extracted from defatted Nannochloropsis oceanica biomass, including electrostatic surface charge, water-absorption and oil-absorption capacities, foaming, emulsion formation and stability, and thermal behaviour, including denaturation and gelation, benchmarked against two widely used commercial proteins such as milk protein (MP) and soybean protein (SP). The water-soluble N. oceanica protein fractions (NP) showed comparable or superior surface charge density, water-absorption capacity, and oil-absorption capacity relative to MP and SP. NP achieved the highest emulsion activity index (131 m2 g−1), the greatest emulsion stability index (121 days), and the most negative zeta potential (−59.3 mV). It also produced the smallest emulsion droplet size among the tested proteins. Its denaturation temperature was 71 °C, indicating good thermal stability. NP formed heat-induced gels at 95 °C with a minimum concentration of 10% (w/w), although the resulting gels were not as firm as those formed by SP. These results indicate that NP possesses emulsifying, thermal, and gelling properties suitable for a range of food applications. Given its sustainable origin and multi-functional performance, NP holds promise as a novel protein ingredient in future foods.
{"title":"Characterisation of water-soluble Nannochloropsis oceanica protein fractions: physical and functional properties","authors":"Thi Phuong Linh Le, Jayani Samarathunga, Katrina Strazdins, Jeroen Rens and Benu Adhikari","doi":"10.1039/D5FB00372E","DOIUrl":"https://doi.org/10.1039/D5FB00372E","url":null,"abstract":"<p >This study presents a comprehensive physical and functional characterisation of water-soluble protein fractions extracted from defatted <em>Nannochloropsis oceanica</em> biomass, including electrostatic surface charge, water-absorption and oil-absorption capacities, foaming, emulsion formation and stability, and thermal behaviour, including denaturation and gelation, benchmarked against two widely used commercial proteins such as milk protein (MP) and soybean protein (SP). The water-soluble <em>N. oceanica</em> protein fractions (NP) showed comparable or superior surface charge density, water-absorption capacity, and oil-absorption capacity relative to MP and SP. NP achieved the highest emulsion activity index (131 m<small><sup>2</sup></small> g<small><sup>−1</sup></small>), the greatest emulsion stability index (121 days), and the most negative zeta potential (−59.3 mV). It also produced the smallest emulsion droplet size among the tested proteins. Its denaturation temperature was 71 °C, indicating good thermal stability. NP formed heat-induced gels at 95 °C with a minimum concentration of 10% (w/w), although the resulting gels were not as firm as those formed by SP. These results indicate that NP possesses emulsifying, thermal, and gelling properties suitable for a range of food applications. Given its sustainable origin and multi-functional performance, NP holds promise as a novel protein ingredient in future foods.</p>","PeriodicalId":101198,"journal":{"name":"Sustainable Food Technology","volume":" 6","pages":" 2226-2238"},"PeriodicalIF":0.0,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/fb/d5fb00372e?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145500558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Márcia Marques, Raquel F. S. Gonçalves, Daniel A. Madalena, Luís Abrunhosa, António A. Vicente and Ana C. Pinheiro
This study aimed to develop plant-based nanoemulsions (NE) for co-encapsulation of vitamin D3 and curcumin, comparing the emulsifying properties of pea and potato proteins. Different oil and protein concentrations were tested, and formulations with the smallest particle size were characterized and submitted to harmonized static in vitro digestion. Formulations prepared with the highest protein concentration (10%) and lowest oil concentration (1%) led to NE with smallest particle size and lowest polydispersity index (PDI). Curcumin and vitamin D3 were successfully co-encapsulated in both protein NE. During digestion, NE showed instability, particularly in the gastric phase, in which an increase in particle size was observed. Pea protein NE exhibited higher curcumin bioaccessibility (76.06 ± 12.05%) than potato protein NE (42.88 ± 3.58%), while the bioaccessibility of vitamin D3 did not show significant differences. Curcumin stability was also higher in pea protein NE (21.44 ± 1.97%) compared to potato protein NE (12.38 ± 1.97%). These results showed that pea protein is more suitable for produced plant-based NE for co-encapsulation of these bioactive compounds. This work contributes to the development of plant-based NE as promising carriers for the co-encapsulation of bioactive compounds, envisaging the fortification of food products, especially plant-based foods, in response to the growing demand for sustainable alternatives.
{"title":"Co-encapsulation of vitamin D3 and curcumin in plant protein-based nanoemulsions: formulation optimization, characterization, and in vitro digestion","authors":"Márcia Marques, Raquel F. S. Gonçalves, Daniel A. Madalena, Luís Abrunhosa, António A. Vicente and Ana C. Pinheiro","doi":"10.1039/D5FB00262A","DOIUrl":"https://doi.org/10.1039/D5FB00262A","url":null,"abstract":"<p >This study aimed to develop plant-based nanoemulsions (NE) for co-encapsulation of vitamin D<small><sub>3</sub></small> and curcumin, comparing the emulsifying properties of pea and potato proteins. Different oil and protein concentrations were tested, and formulations with the smallest particle size were characterized and submitted to harmonized static <em>in vitro</em> digestion. Formulations prepared with the highest protein concentration (10%) and lowest oil concentration (1%) led to NE with smallest particle size and lowest polydispersity index (PDI). Curcumin and vitamin D<small><sub>3</sub></small> were successfully co-encapsulated in both protein NE. During digestion, NE showed instability, particularly in the gastric phase, in which an increase in particle size was observed. Pea protein NE exhibited higher curcumin bioaccessibility (76.06 ± 12.05%) than potato protein NE (42.88 ± 3.58%), while the bioaccessibility of vitamin D<small><sub>3</sub></small> did not show significant differences. Curcumin stability was also higher in pea protein NE (21.44 ± 1.97%) compared to potato protein NE (12.38 ± 1.97%). These results showed that pea protein is more suitable for produced plant-based NE for co-encapsulation of these bioactive compounds. This work contributes to the development of plant-based NE as promising carriers for the co-encapsulation of bioactive compounds, envisaging the fortification of food products, especially plant-based foods, in response to the growing demand for sustainable alternatives.</p>","PeriodicalId":101198,"journal":{"name":"Sustainable Food Technology","volume":" 6","pages":" 2204-2214"},"PeriodicalIF":0.0,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/fb/d5fb00262a?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145500620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Chih-Chun Kuo, Da Chen, Rafael Jiménez-Flores, Macdonald Wick and Osvaldo Campanella
The growing accumulation of food byproducts and waste imposes environmental and economic challenges, highlighting the need for sustainable valorization strategies. This study investigated the usage of fish byproducts and acid whey through lactic acid bacteria (LAB) fermentation to produce bioactive fish protein hydrolysates (FPHs) with potential antioxidant and antibacterial properties. The effects of three formulations: acid whey (FAw), a starter culture (FLr), and their combination (FAwLr) on the fermentation and its products were systematically evaluated. LAB fermentation triggered protein hydrolysis, as demonstrated by the increased degrees of hydrolysis (DH). The fermentation process was also marked by significant microbial growth using FAwLr, with LAB populations above 108 CFU mL−1 by Day 3, accompanied by a rapid pH decline (<4.5). Viscosity of the fermented samples decreased in all formulations, showing smaller consistency indexes and larger flow behavior indexes, indicating enhanced protein hydrolysis and a reduced structural complexity of the system over time. Antioxidant activity, measured by DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid), and FRAP (Ferric Reducing Antioxidant Power) assays, was significantly improved in all formulations containing acid whey. The antibacterial activity against Listeria innocua and Escherichia coli showed formulation-dependent effects. The combination of acid whey and LAB (FAwLr) exhibited the strongest antibacterial properties, lowering both the minimum inhibitory and bactericidal concentrations. These results highlight the synergistic effects of acid whey and LAB in producing multifunctional bioactive hydrolysates with both antioxidant and antibacterial activities, showcasing a sustainable approach to converting waste streams into value-added ingredients.
粮食副产品和废物的日益积累带来了环境和经济挑战,突出了可持续增值战略的必要性。本研究研究了利用鱼类副产品和酸性乳清通过乳酸菌(LAB)发酵生产具有潜在抗氧化和抗菌性能的生物活性鱼蛋白水解物(FPHs)。系统评价了酸乳清(FAw)、发酵剂(FLr)及其组合(FAwLr) 3种配方对发酵及其产物的影响。从水解度(DH)的增加可以看出,乳酸发酵引发了蛋白质水解。发酵过程中也有显著的微生物生长,到第3天,LAB数量超过108 CFU mL - 1,伴随着pH值的快速下降(<4.5)。在所有配方中,发酵后的样品粘度都有所下降,稠度指数变小,流动行为指数增大,表明随着时间的推移,蛋白质水解增强,体系结构复杂性降低。通过DPPH(2,2-二苯基-1-吡啶肼)、ABTS 2,2 ' -氮化氮-(3-乙基苯并噻唑-6-磺酸)和FRAP(铁还原抗氧化能力)测定,所有含有酸性乳清的配方的抗氧化活性都显著提高。对李斯特菌和大肠杆菌的抑菌活性呈配方依赖性。酸性乳清与乳酸菌(falr)的组合具有最强的抗菌性能,降低了最低抑菌浓度和杀菌浓度。这些结果强调了酸乳清和乳酸菌在生产具有抗氧化和抗菌活性的多功能生物活性水解物方面的协同作用,展示了将废物流转化为增值成分的可持续方法。
{"title":"Sustainable valorization of fish byproducts and acid whey through lactic acid bacteria fermentation into bioactive hydrolysates","authors":"Chih-Chun Kuo, Da Chen, Rafael Jiménez-Flores, Macdonald Wick and Osvaldo Campanella","doi":"10.1039/D5FB00444F","DOIUrl":"https://doi.org/10.1039/D5FB00444F","url":null,"abstract":"<p >The growing accumulation of food byproducts and waste imposes environmental and economic challenges, highlighting the need for sustainable valorization strategies. This study investigated the usage of fish byproducts and acid whey through lactic acid bacteria (LAB) fermentation to produce bioactive fish protein hydrolysates (FPHs) with potential antioxidant and antibacterial properties. The effects of three formulations: acid whey (FAw), a starter culture (FLr), and their combination (FAwLr) on the fermentation and its products were systematically evaluated. LAB fermentation triggered protein hydrolysis, as demonstrated by the increased degrees of hydrolysis (DH). The fermentation process was also marked by significant microbial growth using FAwLr, with LAB populations above 10<small><sup>8</sup></small> CFU mL<small><sup>−1</sup></small> by Day 3, accompanied by a rapid pH decline (<4.5). Viscosity of the fermented samples decreased in all formulations, showing smaller consistency indexes and larger flow behavior indexes, indicating enhanced protein hydrolysis and a reduced structural complexity of the system over time. Antioxidant activity, measured by DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid), and FRAP (Ferric Reducing Antioxidant Power) assays, was significantly improved in all formulations containing acid whey. The antibacterial activity against <em>Listeria innocua</em> and <em>Escherichia coli</em> showed formulation-dependent effects. The combination of acid whey and LAB (FAwLr) exhibited the strongest antibacterial properties, lowering both the minimum inhibitory and bactericidal concentrations. These results highlight the synergistic effects of acid whey and LAB in producing multifunctional bioactive hydrolysates with both antioxidant and antibacterial activities, showcasing a sustainable approach to converting waste streams into value-added ingredients.</p>","PeriodicalId":101198,"journal":{"name":"Sustainable Food Technology","volume":" 6","pages":" 1766-1780"},"PeriodicalIF":0.0,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/fb/d5fb00444f?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145500556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Eliara Acipreste Hudson, Herlândia Cotrim Santos, Laís Fernanda Batista, Jaqueline de Paula Rezende, Kely de Paula Correa, Izabela Maria Montezano de Carvalho, Márcia Cristina Teixeira Ribeiro Vidigal and Ana Clarissa dos Santos Pires
The growing demand for sustainable and functional foods has driven the development of novel formulations that both valorize dairy co-products and incorporate functional ingredients. Six fermented dairy beverages were developed by partially replacing skim milk (0–75%, w/w) with buttermilk and/or sweet cheese whey. The formulations were enriched with 1% bee pollen, banana, and honey, fermented using a commercial yogurt culture, and stored at 4 °C. We evaluated the proximate composition, pH and titratable acidity, water-holding capacity (WHC) and syneresis, antioxidant activity, colloidal stability (Turbiscan Stability Index – TSI), rheological behavior, texture, and sensory properties. All formulations exhibited similar acidification profile during storage (pH change from 4.45 to 4.15 and acidity from 0.82 to 0.92% lactic acid over 30 days), indicating unaltered lactose fermentation despite milk replacement. Formulations containing 37.5% of buttermilk or whey achieved higher WHC (>60%) and lower syneresis (∼9–10%), correlating with low TSI values (<0.9) and pseudoplastic rheology. Textural analyses showed that the same formulations achieved a balance between gel strength and cohesiveness, without compromising adhesiveness or elasticity. Antioxidant assays revealed that 75% buttermilk samples exhibited the greatest radical-scavenging activity (DPPH: 72.5 ± 2.1%; ABTS: 63.8 ± 1.9%), underscoring the influence of polar lipids on functionality. Sensory tests pointed to the high acceptance of moderate-replacement samples, linked to creamy, sweet, yogurt-like attributes, whereas 75% whey samples scored lower due to bitterness. Overall, moderate substitution with buttermilk or whey, combined with bee pollen enrichment, produces stable, antioxidant-rich, and consumer-acceptable beverages, supporting functional food development and the circular economy in the dairy sector.
对可持续和功能性食品日益增长的需求推动了新型配方的发展,这些配方既能使乳制品副产品增值,又能纳入功能性成分。用脱脂牛奶和/或甜奶酪乳清部分替代脱脂牛奶(0-75%,w/w),开发了6种发酵乳制品饮料。配方中添加1%的蜂花粉、香蕉和蜂蜜,使用商业酸奶培养物发酵,并在4°C保存。我们评估了其近似组成、pH值和可滴定酸度、保水能力(WHC)和协同作用、抗氧化活性、胶体稳定性(Turbiscan stability Index - TSI)、流变行为、质地和感官特性。所有配方在储存过程中都表现出相似的酸化特征(30天内pH值从4.45变化到4.15,酸度从0.82变化到0.92%),表明尽管更换了牛奶,乳糖发酵没有改变。含有37.5%酪乳或乳清的配方获得了更高的WHC (>60%)和更低的协同作用(~ 9-10%),与低TSI值(<0.9)和假塑性流变学相关。结构分析表明,相同的配方实现了凝胶强度和黏性之间的平衡,而不影响黏性或弹性。抗氧化实验显示,75%的酪乳样品具有最强的自由基清除活性(DPPH: 72.5±2.1%;ABTS: 63.8±1.9%),强调极性脂质对功能的影响。感官测试表明,与奶油、甜味、酸奶类似的特性有关的中度替代样品的接受度很高,而75%的乳清样品由于苦味而得分较低。总的来说,适度替代酪乳或乳清,结合蜂花粉浓缩,生产出稳定、富含抗氧化剂和消费者可接受的饮料,支持功能食品的发展和乳制品行业的循环经济。
{"title":"Sustainable development of dairy-based functional beverages enriched with bee pollen: a circular economy approach","authors":"Eliara Acipreste Hudson, Herlândia Cotrim Santos, Laís Fernanda Batista, Jaqueline de Paula Rezende, Kely de Paula Correa, Izabela Maria Montezano de Carvalho, Márcia Cristina Teixeira Ribeiro Vidigal and Ana Clarissa dos Santos Pires","doi":"10.1039/D5FB00337G","DOIUrl":"https://doi.org/10.1039/D5FB00337G","url":null,"abstract":"<p >The growing demand for sustainable and functional foods has driven the development of novel formulations that both valorize dairy co-products and incorporate functional ingredients. Six fermented dairy beverages were developed by partially replacing skim milk (0–75%, w/w) with buttermilk and/or sweet cheese whey. The formulations were enriched with 1% bee pollen, banana, and honey, fermented using a commercial yogurt culture, and stored at 4 °C. We evaluated the proximate composition, pH and titratable acidity, water-holding capacity (WHC) and syneresis, antioxidant activity, colloidal stability (Turbiscan Stability Index – TSI), rheological behavior, texture, and sensory properties. All formulations exhibited similar acidification profile during storage (pH change from 4.45 to 4.15 and acidity from 0.82 to 0.92% lactic acid over 30 days), indicating unaltered lactose fermentation despite milk replacement. Formulations containing 37.5% of buttermilk or whey achieved higher WHC (>60%) and lower syneresis (∼9–10%), correlating with low TSI values (<0.9) and pseudoplastic rheology. Textural analyses showed that the same formulations achieved a balance between gel strength and cohesiveness, without compromising adhesiveness or elasticity. Antioxidant assays revealed that 75% buttermilk samples exhibited the greatest radical-scavenging activity (DPPH: 72.5 ± 2.1%; ABTS: 63.8 ± 1.9%), underscoring the influence of polar lipids on functionality. Sensory tests pointed to the high acceptance of moderate-replacement samples, linked to creamy, sweet, yogurt-like attributes, whereas 75% whey samples scored lower due to bitterness. Overall, moderate substitution with buttermilk or whey, combined with bee pollen enrichment, produces stable, antioxidant-rich, and consumer-acceptable beverages, supporting functional food development and the circular economy in the dairy sector.</p>","PeriodicalId":101198,"journal":{"name":"Sustainable Food Technology","volume":" 6","pages":" 2321-2336"},"PeriodicalIF":0.0,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/fb/d5fb00337g?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145500565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Wahyu Ramadhan, Joko Santoso, Uju, Rahadiyan Garuda Langit Dewangga, Mario Natanael, Muhammad Aldy Luthfiansyah, Adinda Yulya Rachmawati and Zacky Arivaie Santosa
The demand for biodegradable straws has grown in response to environmental concerns regarding plastic waste. This study aimed to develop and optimize seaweed-based bioplastic straws with enhanced hydrophobicity, mechanical integrity, and biodegradability. Four base formulations combining agar, starch, carrageenan, and konjac were evaluated, with the B9 formulation (comprising agar and starch) selected as the most promising due to its superior thermal stability and low water absorption. This formulation was then used in a process optimization stage, where various homogenization parameters (speed, temperature, and time) were tested. The best-performing straw from this stage, referred to as low-speed-S-B9 (B9 processed under low-speed homogenization), demonstrated the most favorable overall performance, achieving a contact angle of 115.31 ± 1.15°, water absorption below 100.27 ± 2.67%, and tensile strength of 60.76 ± 2.78 MPa. SEM analysis revealed a dense and cohesive matrix structure, while FTIR spectra showed the main polysaccharide functional groups in all samples, with additional peaks reflecting each formulation's chemical composition. Thermal degradation profiles confirmed its heat resistance, with delayed onset and higher char residue. Mechanical and flexural tests showed the low-speed straw maintained high elongation and comparable bending resistance to commercial paper straws. Finally, soil burial testing confirmed full biodegradation of the straw within 60 days. These results confirm that low-speed homogenization of seaweed-based formulations offers a scalable and sustainable strategy to produce biodegradable straws with functional properties suitable for real-world use.
{"title":"Investigation of polymer blend formulation and homogenization dynamics in the development of biodegradable, hydrophobic, and heat-resistant seaweed-based bioplastic straws","authors":"Wahyu Ramadhan, Joko Santoso, Uju, Rahadiyan Garuda Langit Dewangga, Mario Natanael, Muhammad Aldy Luthfiansyah, Adinda Yulya Rachmawati and Zacky Arivaie Santosa","doi":"10.1039/D5FB00344J","DOIUrl":"https://doi.org/10.1039/D5FB00344J","url":null,"abstract":"<p >The demand for biodegradable straws has grown in response to environmental concerns regarding plastic waste. This study aimed to develop and optimize seaweed-based bioplastic straws with enhanced hydrophobicity, mechanical integrity, and biodegradability. Four base formulations combining agar, starch, carrageenan, and konjac were evaluated, with the B9 formulation (comprising agar and starch) selected as the most promising due to its superior thermal stability and low water absorption. This formulation was then used in a process optimization stage, where various homogenization parameters (speed, temperature, and time) were tested. The best-performing straw from this stage, referred to as low-speed-S-B9 (B9 processed under low-speed homogenization), demonstrated the most favorable overall performance, achieving a contact angle of 115.31 ± 1.15°, water absorption below 100.27 ± 2.67%, and tensile strength of 60.76 ± 2.78 MPa. SEM analysis revealed a dense and cohesive matrix structure, while FTIR spectra showed the main polysaccharide functional groups in all samples, with additional peaks reflecting each formulation's chemical composition. Thermal degradation profiles confirmed its heat resistance, with delayed onset and higher char residue. Mechanical and flexural tests showed the low-speed straw maintained high elongation and comparable bending resistance to commercial paper straws. Finally, soil burial testing confirmed full biodegradation of the straw within 60 days. These results confirm that low-speed homogenization of seaweed-based formulations offers a scalable and sustainable strategy to produce biodegradable straws with functional properties suitable for real-world use.</p>","PeriodicalId":101198,"journal":{"name":"Sustainable Food Technology","volume":" 6","pages":" 2282-2296"},"PeriodicalIF":0.0,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/fb/d5fb00344j?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145500562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Gulay Ozkan, Mustafa Tahsin Yilmaz, Elwira Sieniawska, Benita Hryć, Mehmet Çağlar Tülbek and Esra Capanoglu
The high bioactive content of legume by-products enables their utilization in the development of value-added food products. Thus, this study explores the valorization of legume by-products (chickpea hulls (CH), faba bean hulls (FH), and lentil hulls (LH)) through the formulation of novel functional infusions. The effects of in vitro digestion on the phenolic content and antioxidant potential of these infusions were assessed. Aqueous-methanolic (75%) and aqueous extracts of these samples were also evaluated in terms of total phenolic content (TPC), total flavonoid content (TFC), and antioxidant capacity. According to the results, FH and LH showed higher TPC (1002 and 1320 mg GAE/100 g, respectively) and TFC (961 and 986 mg CE/100 g, respectively) values (p < 0.05). Similarly, among the infusions (CHI, FHI, and LHI), which were all prepared from their respective samples, the FHI and LHI exhibited higher levels of TPC, TFC, and antioxidant capacity before and after in vitro digestion (p < 0.05). Additionally, comprehensive LC-ESI-MS/MS phenolic profiling showed the great potential for the retention of individual phenolics in newly formulated infusions. These results suggest that legume by-products have great potential for value-added applications as functional ingredients in infusion formulations, contributing to their sustainable utilization and offering health-promoting properties.
{"title":"Valorization of legume by-products in functional formulations: phytochemicals and their simulated gastrointestinal fate","authors":"Gulay Ozkan, Mustafa Tahsin Yilmaz, Elwira Sieniawska, Benita Hryć, Mehmet Çağlar Tülbek and Esra Capanoglu","doi":"10.1039/D5FB00273G","DOIUrl":"https://doi.org/10.1039/D5FB00273G","url":null,"abstract":"<p >The high bioactive content of legume by-products enables their utilization in the development of value-added food products. Thus, this study explores the valorization of legume by-products (chickpea hulls (CH), faba bean hulls (FH), and lentil hulls (LH)) through the formulation of novel functional infusions. The effects of <em>in vitro</em> digestion on the phenolic content and antioxidant potential of these infusions were assessed. Aqueous-methanolic (75%) and aqueous extracts of these samples were also evaluated in terms of total phenolic content (TPC), total flavonoid content (TFC), and antioxidant capacity. According to the results, FH and LH showed higher TPC (1002 and 1320 mg GAE/100 g, respectively) and TFC (961 and 986 mg CE/100 g, respectively) values (<em>p</em> < 0.05). Similarly, among the infusions (CHI, FHI, and LHI), which were all prepared from their respective samples, the FHI and LHI exhibited higher levels of TPC, TFC, and antioxidant capacity before and after <em>in vitro</em> digestion (<em>p</em> < 0.05). Additionally, comprehensive LC-ESI-MS/MS phenolic profiling showed the great potential for the retention of individual phenolics in newly formulated infusions. These results suggest that legume by-products have great potential for value-added applications as functional ingredients in infusion formulations, contributing to their sustainable utilization and offering health-promoting properties.</p>","PeriodicalId":101198,"journal":{"name":"Sustainable Food Technology","volume":" 6","pages":" 2108-2121"},"PeriodicalIF":0.0,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/fb/d5fb00273g?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145500543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}