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Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology最新文献

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GasPak plus versus anaerocult a — two carbon dioxide/hydrogen systems for cultivation of anaerobes GasPak plus与厌氧菌的对比——两种二氧化碳/氢气系统用于厌氧菌的培养
Wolfgang R. Heizmann , Herbert Werner

Two disposable carbon dioxide/hydrogen gas-generating systems (GasPak Plus and Anaerocult A) were compared by assessing growth of obligate anaerobic bacteria. Eighty strains representing 28 species of anaerobic bacteria commonly occurring at various body sites were seeded onto 4 brain heart chocolate agar plates using a spiral plater; and 1 plate each was subsequently incubated in 2 Anaerocult A and 2 GasPak Plus systems. Bacterial growth was expressed as colony-forming units per ml (CFU/ml), reproducibility of the seeding procedure was checked, and the potential interference of H2S-producing bacteria with operation of the carbon dioxide/hydrogen systems was investigated. The presence of H2S only inhibited bacterial growth in the case of Peptostreptococcus asaccharolyticus — an effect observed in both systems. Reproducibility of the seeding procedure using the spiral plater was within acceptable range. Differences between the systems were not apparent when comparing total CFU/ml of a given species and the systems therefore provide equally effective environments for incubation of anaerobic bacteria. In both systems, however, growth varied from one species or strain to another. In mixed infections, detection of certain species of anaerobes may therefore be difficult using either system.

Zwei Einmalsysteme (GasPak Plus und Anaerocult A) zur Erzeugung einer anaeroben Atmosphäre wurden hinsichtlich der Wachstumsausbeute anaerober Bakterien miteinander verglichen. Die 80 Teststämme (28 Spezies verschiedener Körperregionen) wurden mittels eines Spiralplaters auf vier Hirn-Herz-Kochblutplatten ausgesät und jeweils zwei Platten im Anaerocult-A- bzw. im GasPak-Plus-System bebrütet. Neben dem Parameter der koloniebildenden Einheiten pro ml (KBE/ml) wurde die Reproduzierbarkeit der Ergebnisse des Spiralplaters und der Einfluß H2S-produzierender Bakterien untersucht. Nur bei Peptostreptococcus asaccharolyticus kam es in Gegenwart von H2S in beiden Systemen zu einer Verminderung der KBE/ml. Die Reproduzierbarkeit der Ergebnisse des Spiralplaters war gut. Beim Vergleich der Gesamtzahl der KBE/ml konnten zwischen den beiden Systemen keine Unterschiede festgestellt werden, so daß beide Systeme als äquivalent zu betrachten sind. Die unterschiedliche Bandbreite der KBE/ml, sowohl auf Speziesebene als auch der Stämme innerhalb einer Spezies, wies auf eine Inhomogenität des Wachstums hin. Möglicherweise kann hierdurch bei Mischkulturen die Isolierung einzelner Anerobier erschwert sein.

通过评估专性厌氧细菌的生长情况,比较了两种一次性二氧化碳/氢气生成系统(GasPak Plus和Anaerocult A)。采用螺旋培养皿将80株代表28种常见于身体不同部位的厌氧细菌接种到4个脑心巧克力琼脂板上;随后分别在2个Anaerocult A和2个GasPak Plus系统中孵育1个板。细菌生长以菌落形成单位/ml (CFU/ml)表示,检查播种过程的可重复性,并研究产生h2s的细菌对二氧化碳/氢气系统操作的潜在干扰。H2S的存在仅在溶糖胃链球菌的情况下抑制细菌生长-在两种系统中都观察到这种作用。螺旋播种机播种过程的再现性在可接受的范围内。当比较给定物种的总CFU/ml时,系统之间的差异并不明显,因此系统为厌氧细菌的孵化提供了同样有效的环境。然而,在这两种系统中,不同物种或菌株的生长情况各不相同。因此,在混合感染中,使用任何一种系统都难以检测某些种类的厌氧菌。zweiemalsysteme (GasPak Plus and Anaerocult A) zur Erzeugung eener厌氧剂Atmosphäre wurden hinsichtlich der wachstsausbeute厌氧剂Bakterien mitenderverglichen。Die 80 Teststämme (28 Spezies verschiedener Körperregionen) wurden mittelels eines Spiralplaters auf vier Hirn-Herz-Kochblutplatten ausgesät和珠宝zwei Platten in anaerobert - a - bzw。我是gaspak - plus system。Neben dem Parameter der koloniebildenden enheiten proml (KBE/ml) wurde die reproduction zierbarkeit der Ergebnisse des Spiralplaters and der influß h2s - produczierender Bakterien untersucht。北碚胃解糖链球菌在北碚细菌系统中对H2S链球菌的抑制作用(KBE/ml)。Die reproductive zierbarkeit der Ergebnisse des Spiralplaters war gut。bem Vergleich der Gesamtzahl der KBE/ml konten zwischen den bebeen Systemen keine Unterschiede festgestellt werden,所以bebeide Systeme也äquivalent zu betrachten sind。Die unterschiedliche Bandbreite der KBE/ml,所以wohl auf Speziesebene也auch der Stämme innerhalb einer Spezies, wohl auf eine Inhomogenität des Wachstums hin。Möglicherweise kankanhierdurch bei Mischkulturen die Isolierung einzerner erschwert sein。
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引用次数: 5
Lectinophagocytosis mediated by bacterial surface lectins 细菌表面凝集素介导的凝集素吞噬作用
Itzhak Ofek

The evidence showing that non-opsonic recognition of bacteria by phagocytes involve interaction between bacterial surface lectin and sugars on the phagocytic cells is summarized. This process, termed lectinophagocytosis, probably occur in vivo as evident from experimental infections with mixed phenotypes one of which express mannose specific (MS) lectin which mediate lectinophagocytosis of the bacteria and the other does not. In all cases studied, the lectin bearing phenotype survived better in phagocytes-poor sites and the phenotype which does not express this lectin survived better in phagocytes-rich sites. Due to the phase variation phenomenon, an off-on switch allowing the bacterial clone to alternate between lectin expressing and non-expressing phenotypes, the invading bacteria grow as a mixture of phenotypes. The phenotype expressing fimbrial lectin for which receptors are accessible on phagocytic cells undergo lectinophagocytosis. The phenotypes not expressing fimbrial lectin or expressing lectin for which receptors are not available on phagocytic cells may escape phagocytosis and proliferate. It is postulated that pathogenesis of inflammation and tissue damage following infections with MS lectin bearing bacteria may be partly due to both bacterial proliferation resulting in the release of toxic products and to lectinophagocytosis associated with the release of inflammatory agents.

综述了吞噬细胞对细菌的非声速识别涉及细菌表面凝集素与吞噬细胞上的糖相互作用的证据。这一过程被称为凝集素吞噬作用,可能发生在体内,从混合表型的实验感染中可以明显地看到,其中一种表型表达甘露糖特异性(MS)凝集素,介导细菌的凝集素吞噬作用,而另一种则没有。在所有研究的病例中,含有凝集素的表型在吞噬细胞缺乏的位点存活得更好,而不表达这种凝集素的表型在吞噬细胞丰富的位点存活得更好。由于相位变化现象,一个开关允许细菌克隆在表达凝集素和不表达表型之间交替,入侵细菌以混合表型生长。在吞噬细胞上可接触到的表达毛状凝集素受体的表型发生凝集吞噬作用。不表达纤毛凝集素或表达凝集素受体在吞噬细胞上不可用的表型可能逃避吞噬和增殖。据推测,携带MS凝集素的细菌感染后的炎症和组织损伤的发病机制可能部分是由于细菌增殖导致毒性产物的释放和与炎症因子释放相关的凝集素吞噬作用。
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引用次数: 1
Demonstration of capsules in Clostridium difficile 艰难梭菌胶囊的演示
Elke Strelau , Barbara Wagner , Manfred Wagner , Wolfgang Karsch

In four strains of Clostridium difficile the formation of capsules was demonstrated by light and electron microscopy.

Bei vier Stämmen von Clostridium difficile wurde durch Licht- und Elektronenmikroskopie eine Kapselbildung nachgewiesen.

冷酷得令人难以置信。在四种氯碘化物中间,有光和电子显微镜显示一个胶囊产生。
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引用次数: 5
The significance of the Ipazyme IgA and IgG antibody test in the diagnosis of urogenital chlamydial infections Ipazyme IgA和IgG抗体检测在泌尿生殖道衣原体感染诊断中的意义
Helmut Näher , Detlef Petzoldt

Urogenital specimens from 200 male and female patients were cultivated for the detection of C. trachomatis. For comparison, serum of the same patients was investigated with the Ipazyme IgA and IgG test. This comparison of culture and serological tests revealed a sensitivity of the IgA Ipazyme test of 56% and a specificity of 81%. For the IgG Ipazyme test, the corresponding values were 77% and 39%. The sensitivity of the conventional immunofluorescence test reached 46% and its specificity was 50% for the same group of patients. Antibiotic treatment of 13 IgA-positive patients resulted in a significant decrease of the titre in only one case. The introduction of the Ipazyme test does not open a new aspect in chlamydial serology, i.e. the diagostic value of serology for the detection of a current chlamydial infection remains low.

Das Ergebnis der Anzüchtung von C. trachomatis aus urogenitalem Abstrichmaterial von 200 Patienten und Patientinnen wurde mit dem Ergebnis des Ipazyme IgG- und IgA-Antikörpertests bei der Untersuchung der zugehörigen Seren verglichen. Für den Ipazyme IgA-Test wurde eine Sensitivität von 56% ermittelt, die Spezifität lag bei 81%. Die entsprechenden Parameter betrugen für den Ipazyme IgG-Test 77% bzw. 39%. Zum Vergleich: Die Sensititivät des konventionellen IgG-IFT lag bei der identischen Klientel bei 46% und die Spezifität bei 50%. Eine antibiotische Therapie bei 13 IgA-positiven Patienten führte innerhalb von 3–11 Monaten nur in einem Fall zu einem signifikanten Titerabfall. Durch die Einführung des Ipazyme-Tests ergibt sich kein neuer Aspekt für die Chlamydiendiagnostik, d.h. daß die Serologie nach wie vor für die Diagnose einer bestehenden urogenitalen Chlamydieninfektion nur eine untergeordnete Rolle spielt.

对200例男性和女性患者的泌尿生殖道标本进行培养,检测沙眼衣原体。为比较,对同一组患者的血清进行Ipazyme IgA和IgG检测。这种培养和血清学试验的比较显示IgA Ipazyme试验的敏感性为56%,特异性为81%。IgG Ipazyme检测对应值分别为77%和39%。常规免疫荧光检测对同一组患者的敏感性为46%,特异性为50%。13例iga阳性患者的抗生素治疗仅导致1例滴度显著降低。Ipazyme试验的引入并没有为衣原体血清学开辟一个新的方面,即血清学在检测当前衣原体感染方面的诊断价值仍然很低。摘要资料200patienten和patientinen wurde mitdem Ergebnis des Ipazyme IgG- und IgA-Antikörpertests beder Untersuchung der zugehörigen七verglichen。 r den Ipazyme IgA-Test wurde eine Sensitivität von 56%, die Spezifität lag bei 81%。试验参数:血清igg检测77% bzw。39%。Zum Vergleich: Die Sensititivät des conventionellen IgG-IFT lag bei der identischen Klientel 46%, Die Spezifität 50%。抗生素治疗13例iga阳性患者,3-11例腹腔内腔出血,腹腔内滴度明显下降。德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国
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引用次数: 1
Quantitative assessment of human neutrophil chemiluminescence induced by opsonized Escherichia coli K-12 调理大肠杆菌K-12诱导人中性粒细胞化学发光的定量评价
Thomas W. Jungi , Anton Schmid , Andreas Morell , Peter J. Spaeth , Ernst Peterhans

The interaction of opsonized E. coli K-12 bacteria and polymorphonuclear leukocytes (PMN) was quantified, using luminol-enhanced chemiluminescence (CL) as a parameter of PMN stimulation. On a double-logarithmic scale light emission depended on the opsonin concentration used during pre-opsonisation. The most potent CL-inducing agent was fresh human serum, and its stimulatory activity depended on an intact complement (C) system. Both inactivation of C by heating or blocking the classical C pathway with EGTD decreased the CL-inducing potency by a factor of 8 to 16. Hypogammaglobulinemic heated serum mediated little CL. IgG for intravenous use mediated CL generation, but reduction/alkylation and sulphitolysis reduced the stimulatory power. Evidence is presented that the anti-K-12 antibodies within commercial IgG and IgM used for substitution do not improve the stimulatory power of IgG-deficient, IgM- and C-sufficient serum, unless very high Ig concentrations are substituted.

利用鲁米诺增强的化学发光(CL)作为PMN刺激的参数,定量了活化的大肠杆菌K-12细菌与多形核白细胞(PMN)的相互作用。在双对数尺度上,光发射取决于预调理期间使用的调理素浓度。最有效的cl诱导剂是新鲜人血清,其刺激活性依赖于完整的补体(C)系统。通过加热或用EGTD阻断经典C途径使C失活,可使cl诱导效力降低8 - 16倍。低γ球蛋白热血清介导的小CL。静脉注射IgG介导CL的生成,但还原/烷基化和硫酸溶解降低了刺激能力。有证据表明,用于替代的商业IgG和IgM中的抗k -12抗体不能提高IgG缺乏,IgM和c充足的血清的刺激能力,除非替代非常高的Ig浓度。
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引用次数: 2
Mikrobielle besiedlung kariöser progressionsstadien im dentin menschlicher zähne — eine kontrollierte therapiestudie 一种克隆微生物身上的蛀牙
Susanne Kneist, Roswitha Heinrich, W. Künzel

In a controlled clinical trial the microflora of the cavity floor of 70 primary lower second molars with deep carious lesions was determined after caries excavation. The teeth were extracted and pulpal status was evaluated after 16 month of microbial control to determine the etiopathogenic role of germs for carious progression in dentine.

67% of the primary molars were free from pulpal inflammations. Soft carious dentine were significantly higher infected than the clinically acceptable hard dentine. Only in 40% of the cavity floors the microorganisms were eliminated. In the infected teeth basophilic microorganisms were found in causality to pulps without inflammations; acidogenic streptococci and lactobacilli were involved in pulpal inflammations. Results indicate that the latter genera of microorganisms are of etiological significance for carious progression in dentine.

In einer klinisch-kontrollierten Therapiestudie zur Vitalerhaltung des Milchzahnendo-dontes wurde die Mikroflora am Kavitätenboden von 70 zweiten unteren Milchmolaren mit symptomloser Caries profunda nach Kariesexkavation erfaßt und ihre ätiopathogenetische Bedeutung für die Kariesprogression in das Dentin durch nachfolgende morphologische Untersuchungen der Zähne nach einem Therapiezeitraum von 16 Monaten bewertet. 67% der Milchmolaren zeigten einen entzündungsfreien Pulpastatus.

Geringgradig erweichtes Dentin erwies sich im Vergleich zu klinisch akzeptablen harten Dentin des Kavitätenbodens als deutlich höher besiedelt und nur 40% der Kavitätenboden waren nach mikrobiologischer Kontrolle befundfrei.

In den noch geringgradig infizierten Zähnen korrelierte aber eine entzündungsfreie Pulpa mit einem basophilen Besiedlungsmuster und acidogene und acidurische Streptokokken (S. mutans) und Laktobazillen wurden bei chronischen Pulpainflammationen aufgefunden, so daß letztere Gattungen von Mikroorganismen ätiopathogenetish für die kariöse Progression in das Dentin bedeutungsvoll sein dürften.

本文对70颗深龋的初生下第二磨牙进行了牙槽菌群的测定。拔牙后进行16个月的牙髓微生物控制,以确定细菌对牙本质龋齿进展的致病作用。67%的初生磨牙无牙髓炎症。软质牙本质感染明显高于临床可接受的硬质牙本质。只有40%的空腔地板微生物被清除。在感染牙中发现嗜碱性微生物与无炎症的牙髓有因果关系;致酸性链球菌和乳酸菌参与了牙髓炎症。结果表明,后一类微生物对牙本质龋病的发展具有病原学意义。在einer klinisch- control - liberiestudie zur Vitalerhaltung des milchzahnendotes -dontes wurde die microflora am Kavitätenboden von 70 zweiten unteren Milchmolaren mit。67% der Milchmolaren zeigten einen entz ndungsfreien Pulpastatus。geringgradiingerweichtes Dentin eries (Vergleich zu klinisch akzeptablen harten)牙牙学研究(Vergleich zklinisch akzeptablen harten)牙牙学研究(Vergleich zklinisch) Kavitätenbodens牙牙学研究(als deutlich höher牙牙学研究)In den noch geringgradig infizierten Zähnen korrelierte aber eine entzndungsfreie Pulpa mit einem basophilen besiedlungsmaster und acid - ogene und acid - urische Streptokokken (S. mutans) und Laktobazillen wurden bei chronischen pulpainationen aufgefunden, so dasß letztere Gattungen von microorganismen ätiopathogenetish fr die kariöse das Dentin bedeutungsvoll sein dten。
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引用次数: 3
Pathogenicity testing of listeria strains isolated from food in fertilized hen's eggs 从食物中分离的李斯特菌菌株在受精卵中的致病性检测
Christine Lattmann , Andreas Schwarzkopf , Heinz P.R. Seeliger

Pathogenicity testing of 10-day-old fertilized hen's eggs infected with Listeria species demonstrated that L. monocytogenes isolated from cheese and human samples caused death in 100% of the injected chicken embryos within 96 h. In contrast, L. innocua isolated from cheese was fatal for only 17% of the infected embryos. All embryos infected with L. seeligeri survived. The results confirmed statements of previous authors about the pathogenicity of L. monocytogenes, regardless of its origin.

对10日龄受精卵感染李斯特菌的致病性测试表明,从奶酪和人样品中分离的单核细胞增生乳杆菌在96 h内使注射的鸡胚胎100%死亡,而从奶酪中分离的无性乳杆菌仅对感染的胚胎致死率为17%。所有感染了seeligeri的胚胎都存活了下来。结果证实了以前作者关于单核增生乳杆菌致病性的陈述,而不管其来源如何。
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引用次数: 6
Levels of toxic shock syndrome toxin-1 production among staphylococcus aureus strains and clinical implications 金黄色葡萄球菌菌株中毒性休克综合征毒素-1产生水平及其临床意义
A. Satyanarayan Naidu , Carl Kamme, Åsa Ljungh, Torkel Wadström

Among 250 S. aureus clinical isolates, the incidence of TSST-1 production was 18.0%. S. aureus var. hominis strains were predominant (95.6%), producing high levels of toxin in vitro, within the range of 0.6 to 4.3 μg/ml and exhibiting crystal violet binding with C/D pattern. No correlation was found between the level of TSST-1 production in vitro and the clinical course. Two (3.4%) of the var. bovis strains produced toxin in amounts less than 0.6 μg/ml and did not bind crystal violet. None of the 24 var. canis isolates produced TSST-1. Fifty five per cent of the isolates of phage group I produced TSST-1 and corresponded to 57.8% of the toxigenic strains. Two of the 250 patients developed toxic shock syndrome.

Die Häufigkeit der Bildung von TSST-1 bei 250 klinischen Isolaten von S. aureus betrug 18,0%. Es herrschten S. aureus var. hominis-Stämme vor (95,6%), die in vitro hohe Toxin-spiegel (Bereich 0,6–4,3 μg/ml) bildeten und Kristallviolettbildung mit einem C/D-Muster zeigten. Es wurde keine Korrelation zwischen der Höhe der TSST-1-Bildung in vitro und dem klinischen Verlauf festgestellt. Zwei (3,4%) der var bovis-Stämme bildeten Toxin in Mengen, die unter 0,6 μg/ml lagen und Kristallviolett nicht banden. Keines der 24 var. canis-Isolate bildete TSST-1. 55% der Isolate der Phagengruppe I bildete TSST-1, was 57,8% der toxigenen Stämme entsprach. Bei zwei der 250 Patientinnen entwickelte sich ein “Toxic Shock”-Syndrom.

250株金黄色葡萄球菌临床分离株中,TSST-1产生率为18.0%。金黄色葡萄球菌(S. aureus var. hominis)菌株占优势(95.6%),体外毒素含量较高,在0.6 ~ 4.3 μg/ml范围内,呈结晶紫结合,呈C/D模式。体外TSST-1分泌水平与临床病程无相关性。其中2株(3.4%)产毒量小于0.6 μg/ml,不与结晶紫结合。24株犬变种分离株均未产生TSST-1。55%的噬菌体I组分离物产生TSST-1,对应57.8%的产毒菌株。250名患者中有2人出现了中毒性休克综合征。Die Häufigkeit der Bildung von TSST-1 bei 250 klinischen Isolaten von S. aureus beg 18.0%。金黄色葡萄球菌(Es herrschten S. aureus var. hominis-Stämme vor)(95.6%)在体外用毒素-明镜(Toxin-spiegel) (Bereich 0,6 - 4,3 μg/ml)培养剂和水晶紫(Kristallviolettbildung)培养剂培养C/D-Muster zeigten而死亡。[2][1][1][1][1][1][1][1][1][1][1][1]。Zwei (3.4%) der var bovis-Stämme bildeten Toxin在孟根中,在0.6 μg/ml lagen和水晶紫夜药作用下死亡。Keines der 24 var. canis-Isolate可检测tst -1。55%的分离物der Phagengruppe I bildete tst -1, 57.8%的der toxigenen Stämme entsprach。“中毒性休克”综合征250例。
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引用次数: 5
Duck hepatitis B virus: Cloning and subcloning of the viral genome 鸭乙型肝炎病毒:病毒基因组的克隆和亚克隆
Konrad Oexle, Hubert E. Blum , Eike Walter, Wolf-Bernhard Offensperger, Silke Offensperger, Hajo Grundmann, Karl Teubner, Wolfgang Gerok

In the course of studies on the biology of hepadnavirus infections, duck hepatitis B virus (DHBV) DNA was isolated from the serum of a German Pekin duck. Viral DNA was cloned in E. coli using pBR 322 DNA as a vector. The cloned DHBV DNA F 12 was characterised by restriction enzyme analyses.

DHBV DNA F 1–6 was subcloned in both orientations in plasmid pSP 65 to produce strand-specific RNA probes. These probes specifically identified asymmetrically replicating nascent minus-strand DHBV DNA species or plus-strand viral RNA transcripts.

Im Rahmen von Studien zur Biologie der Hepadnavirus-Infektionen wurde Entenhepatitis B Virus (DHBV) DNA aus einer deutschen Pekingente isoliert. Mit Hilfe des Vektors pBR 322 wurde die virale DNA in E. coli kloniert. Die klonierte DHBV DNA F 1–6 wurde durch Restriktionsanalysen charakterisiert.

Zur Herstellung von strang-spezifischen RNA Gensonden wurde DHBV DNA F 1–6 im Plasmid pSP 65 in beiden Orientierungen subkloniert. Mit diesen RNA Gensonden wurden naszierende Minusstränge der sich asymmetrisch replizierenden DHBV DNA bzw. virale RNA Transkripte von Plusstrang-Polarität spezifisch nachgewiesen.

在大脑传染病生物学、乙型肝炎病毒中,DNA已经被一种德语杜根鸭血清隔离了。去吧,那是大肠杆菌此外,它是大肠杆菌cloned型DNA符合要求dhv DNA f16是用于等离子pSP 65生产炸药的both东方注射器这种不对称的自我复制型在对赫柏氏细菌感染的生物学进行的研究中,鸭B病毒(dh乙肝)被从德国北京病毒中分离出来。322房在水温斑马的指导下复制的dh6型DNA是通过限制分析确定的。为产生特定的物质RNA genonden,在两项指示中均复制了dhv DNA f16通过这些genna的探针,我们可以通过特定的、非对称的dhda DNA或病毒RNA复制的颗粒序列号去了。
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引用次数: 0
Investigation on monoclonal antibodies against two serovars of the icterohaemorrhagiae serogroup of leptospira 钩端螺旋体黄疸出血型两种血清型单克隆抗体的研究
Zhang Wan-He , Bai Xiu-Fen, Deng Qing-Dong, Nie Di-Kai

By means of the cell fusion technique, two hybridoma cell lines, V-1 and H2-1 have been obtained. V-1 cells secrete monocloncal antibody against serovars icterohaemorrhagiae and dakota. The H2-1 cell line secretes serovar-specific monoclonal antibody against serovar H2.

These monoclonal antibodies have been successfully used in serovar-typing of leptospires isolated in China. The results of identification of leptospires by using monoclonal antibodies showed total coincidence with that by the traditional cross agglutinin absorption test and factor antiserum method.

It was confirmed by using monoclonal antibody that the serological agglutination totally paralleled with animal protection. On the basis of the study, a concept was proposed that the agglutination in vitro and the protection in vivo are different manifestations in different reaction systems from the same antibody (antibodies) stimulated by a component(s) of the surface antigen of leptospires.

通过细胞融合技术,获得了V-1和H2-1两个杂交瘤细胞株。V-1细胞分泌单克隆抗体对抗黄疸出血热和达科他。H2-1细胞系分泌针对血清H2的血清特异性单克隆抗体。这些单克隆抗体已成功用于钩端螺旋体的血清分型。单克隆抗体鉴定钩端螺旋体的结果与传统的交叉凝集素吸收试验和因子抗血清法鉴定结果完全吻合。单克隆抗体证实其血清学凝集与动物保护完全一致。在此基础上,提出钩体表面抗原某一组分刺激同一抗体在不同反应体系中的不同表现,即体外凝集和体内保护。
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引用次数: 3
期刊
Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology
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