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Synergistic Effect of Capparis Spinosa Fruits Extract in Comparison with Ciprofloxacin Against Resistant E. Coli O157:H7 刺荆果提取物与环丙沙星对耐药大肠杆菌O157:H7的协同作用比较
Pub Date : 2022-11-06 DOI: 10.54174/utjagr.vo11.n2/26
Ali H. Saliem, Reham Najem Abdulridha
This study was conducted to evaluate the synergistic effect between Capparis Spinosa fruits methanolic extract and ciprofloxacin against resistant E. Coli O157:H7, in varying concentration. This experiment was carried out through the ultrasonic alcoholic extraction of C. Spinosa fruits, and an extraction ratio of 24% was obtained. The extract showed pronounced concentration dependent antibacterial activity. The susceptibility study revealed that E. Coli O157:H7 was sensitive to C. Spinosa fruits. The findings of the present study indicate that the use of pronounced C. Spinosa fruits extract may have the perfect to be choice in clinical control. The concentration of C. spinosa fruits extract increase the activity of inhibition, increase as well when used in combination with ciprofloxacin (half concentration from each other). In result appeared synergism effect between plant extract and ciprofloxacin and may make bacteria lost their resistance to antibiotics by this synergism phenomenon. The result of MIC (6400 µg/ ml), MBC (12800 µg/ ml) and for C. spinose fruits extract while ciprofloxacin MIC (12.5 µg/ ml) and MBC (25 µg/ ml). MICs of CIP/ C. spinosa fruits extract combinations against E. coli O157:H7 isolate was 1.562/ 1600 μg/ml. Value of minimum bactericidal concentration (MBC) of the synergistic combinations  (CIP/ C. spinosa fruits extract) was 3.124/ 3200 μg/ml. According to obtained results fractional inhibitory concentration (FIC) value of (CIP/ C. spinosa fruits extract 1.562/ 1600 μg/ml) was (0.375) less than 0.5 indicates synergistic effect of interaction.
研究了不同浓度刺荆果甲醇提取物与环丙沙星对耐药大肠杆菌O157:H7的协同作用。本实验通过超声波酒精提取棘果,提取率为24%。提取物具有明显的浓度依赖性抗菌活性。结果表明,大肠杆菌O157:H7对棘果敏感。本研究结果表明,棘果提取物在临床控制中具有良好的选择价值。棘果提取物的浓度增加了抑制活性,与环丙沙星(各占一半浓度)联用也增加了抑制活性。结果表明,植物提取物与环丙沙星之间存在增效作用,可能使细菌失去对抗生素的耐药性。MIC(6400µg/ ml)、MBC(12800µg/ ml)和棘果提取物,环丙沙星MIC(12.5µg/ ml)和MBC(25µg/ ml)的测定结果。CIP/刺果提取物组合对大肠杆菌O157:H7的mic值为1.562/ 1600 μg/ml。增效组合(CIP/刺果提取物)的最小杀菌浓度(MBC)为3.124/ 3200 μg/ml。结果表明,CIP/棘果提取物1.562/ 1600 μg/ml的分数抑制浓度(FIC)值为(0.375)< 0.5,表明其具有协同作用。
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引用次数: 0
Studying the relationship between a number of carcass traits and their regression on live weight in sheep 研究绵羊若干胴体性状及其对活重的回归关系
Pub Date : 2022-11-06 DOI: 10.54174/utjagr.vo11.n2/21
Haneen Sattar Abbas, Abdullah Hameed Salim
This study was conducted in Suq Al-Shuyoukh district, south of Thi Qar province, for the period from 5/11/2021 to 8/5/2022 on a sheep sample consisting of 49 local Iraqi sheep (Arabi ) from one of the private sector herds.The study included slaughtering sheep in a butcher's shop, recording the traits of the carcass and the weight of the thigh inside the shop, and then taking a sample of the thigh meat to study some of its chemical traits .Chemical tests were conducted on the meat sample in the laboratories of the Faculty of Agriculture and the Marshes of the University of Thi Qar, and the genetic material was extracted and genetic analysis was done in the laboratory of the Marsh Research Center at the University of Thi Qar. We reached the following results: The traits of hot carcass weight, carcass length, carcass diameter, and thigh weight showed a highly significant and positive correlation among them, while the rest of the traits differed in their correlations in terms of morality or in terms of being negative or positive. Through the results of the statistical analysis, it was found that the value of the regression coefficient for the characteristics of hot carcass weight, carcass length, thigh weight, dressing percentage, pH of meat, and muscle fiber diameter on the live weight of the animal was positive (except for the dressing percentage) and highly significant (P≤0.01).
本研究于2021年11月5日至2022年8月5日期间在Thi Qar省南部的Suq Al-Shuyoukh地区进行,样本为49只来自私营部门牧群的当地伊拉克羊(Arabi)。这项研究包括在一家肉店屠宰羊,记录胴体的特征和羊腿在肉店里的重量,然后采集羊腿肉的样本来研究它的一些化学特征。肉样本在Thi Qar大学农业学院和沼泽学院的实验室进行了化学测试。遗传物质被提取出来并在Thi Qar大学Marsh研究中心的实验室进行遗传分析。结果表明:热胴体重、胴体长、胴体直径和腿重三者之间呈极显著正相关,其余性状与道德的相关性或正相关或负相关存在差异。通过统计分析结果发现,热胴体重、胴体长、大腿重、屠宰率、肉pH、肌纤维直径等性状对活重的回归系数除屠宰率外均为正且极显著(P≤0.01)。
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引用次数: 0
Biochemical and Histopathological Effects Induced by Methimazole in Rats Treated by vitamin C 甲巯咪唑对维生素C处理大鼠的生化及组织病理学影响
Pub Date : 2022-11-06 DOI: 10.54174/utjagr.vo11.n2/25
Sabreen Majid Mohammed, Mohammed Hussein Hindi, Ali Husein Ali Al-Safar
In order to investigate some biochemical and histopathological changes effects of Methimazole (ME)in rats twenty-eight white female rats were equally divided into four target groups, (ME) The 1st group, was given sterile saline (0.9%) and served as a control (c). The 2nd group, was given Methimazole at dose 0.04 mg/kg B.W. orally (positive control). The 3rd group, was given Methimazole at dose 0.04 mg/kg B.W. and Vitamin C 200 mg/kg B.W. orally. The 4th group, was given only Vitamin C 200 mg/kg B.W. orally. Results aimed in the present study on the effect of Methimazole at daily intake doses to investigate possible adverse effects and modulate its possible mechanism by measuring the oxidant and antioxidant impact in brain and Ameliorative trail of vit. C. Vit C antioxidant, such as super oxide, increase super oxide elimination. examination revealed that the (ME) histopathological figures showed very small size thyroid gland that displayed atrophy, composed numerous small size follicles with normal follicular cells and C-cells. Study concludes, (vit c) can decreased thyroid damage caused by oxidative effects triggered by (ME) and linked to their antioxidant effects.
为了观察甲巯咪唑(ME)对大鼠的一些生化和组织病理学变化的影响,将28只雌性白种大鼠随机分为4个靶组,(ME)第一组给予0.9%无菌生理盐水作为对照(c),第二组给予0.04 mg/kg体重的甲巯咪唑口服(阳性对照)。第三组患者口服甲巯咪唑0.04 mg/kg体重,维生素C 200 mg/kg体重。第四组只给予维生素C 200 mg/kg B.W.口服。结果本研究旨在通过测定甲巯咪唑对脑内氧化和抗氧化的影响以及对维生素d的改善作用,探讨每日摄入甲巯咪唑可能的不良反应和调节其可能的机制。C.维生素C抗氧化剂,如超氧化物,增加超氧化物消除。检查显示(ME)组织病理学图显示甲状腺体积很小,呈萎缩状,由大量小滤泡组成,滤泡细胞和c细胞正常。研究得出结论,维生素c可以减少由ME引起的氧化作用引起的甲状腺损伤,并与它们的抗氧化作用有关。
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引用次数: 0
Effect of spraying with Ferti Mar and GROWX AMINO MIX on some chemical characteristics of Citrus aurantium seedlings Citrus aurantium L. 喷施Ferti Mar和GROWX AMINO MIX对柑桔幼苗化学特性的影响。
Pub Date : 2022-11-03 DOI: 10.54174/utjagr.vo11.n2/15
Mahmoud S. Abdel-Wahed, Mohammed Ghani Mohsen, Ahmed Salamjwar
The experiment was carried out in the Saran-covered nursery of the Department of Horticulture and Landscaping - College of Agriculture and Marshes at Dhi Qar University during the growing season (2021-2022). One-year-old citrus seedlings were obtained as homogeneous in growth as possible from one of the Baghdad governorate nurseries on February 20. 2022 The seedlings were transferred to plastic pots with a capacity of 10 kg (26 cm diameter) filled with an agricultural medium consisting of mixed soil and moss in a ratio of 1:3. The process of servicing the plants was carried out in a similar way, such as pruning, hoeing, fertilizing and irrigation throughout the study period, the study was carried out as a two-factor experiment using the design of sectors Complete Randomization (R.C.B.D)‚ The first factor represented the spraying with Ferti Mar compound at four concentrations (1.5,1,0.5,0) ml per liter-1 and the second factor spraying with the GROWX AMINO MIX compound at four concentrations (0, 1, 2, 3) g per liter-1, and it was sprayed at a rate of three sprays Between one spray and another two weeks, and the results of the study can be summarized as follows: Ferti mar spraying treatments showed a positive effect on most chemical indicators of citrus seedlings. The spraying treatment with a concentration of 1.5 ml L-1 significantly outperformed by registering the highest rate for the character and content of leaves from total chlorophyll, carbohydrates and content of leaves from elements The nutritional value of nitrogen, phosphorous and potassium was 59.89 mg, 100 g-1 fresh weight, 11.260%, 1.83%, 0.24%, and 1.98%, respectively.Spraying treatments with Growx amino mix showed a positive effect on most of the chemical indicators of citrus seedlings. The spraying treatment with a concentration of 3 gm l-1 was superior by recording the highest rate of chlorophyll in leaves, carbohydrate percentage in leaves and leaf content of nutrients nitrogen, phosphorous, potassium, which amounted to 59 .88 mg 100 g-1 mw, 11.361%, 1.80%, 0.24%, 1.89%, respectively.The interaction treatments between the two factors of the study gave a positive significant effect in most of the studied chemical characteristics. The interaction treatment between the concentration of 1.5 ml L-1 of ertimar and 3 gm L-1 Growx amino mix recorded the best significant results for growth indicators.
该试验于生长季节(2021-2022年)在迪尔卡尔大学农业与沼泽学院园艺与景观学系的萨兰覆盖的苗圃中进行。2月20日,从巴格达省的一个苗圃中获得了一岁的柑橘幼苗,其生长尽可能均匀。幼苗被移栽到容量为10公斤(直径26厘米)的塑料罐中,罐中填充由土壤和苔藓混合组成的农业介质,比例为1:3。维修过程中植物以类似的方式进行,如修剪、除草,施肥和灌溉在整个研究期间,这项研究是进行双因素实验使用的设计部门完成随机化(R.C.B.D),第一个因素代表了喷洒Ferti Mar化合物在四个浓度(1.5、1、0.5,0)毫升每liter-1和第二个因素喷涂GROWX氨基化合物混合在四个浓度(0,1,2,3)g / liter-1,喷施次数为3次,每2周喷一次,结果表明:喷施处理对柑桔幼苗的大部分化学指标均有正向影响。浓度为1.5 ml L-1的喷施处理对叶面总叶绿素含量、碳水化合物含量和元素含量的影响最大,氮、磷、钾的营养价值分别为59.89 mg、100 g-1鲜重、11.260%、1.83%、0.24%和1.98%。喷施Growx胺基混合物对柑橘幼苗的大部分化学指标均有显著的正向影响。喷施浓度为3 gm l-1时,叶片叶绿素率、叶片碳水化合物率和叶片氮、磷、钾养分含量最高,分别为59.88 mg 100 g-1 mw、11.361%、1.80%、0.24%、1.89%。本研究中两个因素的交互处理对所研究的大部分化学特性都有显著的正向影响。以浓度为1.5 ml L-1的埃替玛与3 gm L-1的Growx氨基酸混合物互作处理的生长指标效果最好。
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引用次数: 0
Effect of CAPN1 gene polymorphism on some physical and sensory traits in broiler meat CAPN1基因多态性对肉鸡部分生理和感官性状的影响
Pub Date : 2022-11-03 DOI: 10.54174/utjagr.vo11.n2/12
Ali Ahmed Abdul-Kareem, Abdul-Kadhum Dakhil Raysan
This study was conducted in the poultry field, Animal Production Department, College of Agriculture and Marshes, University of Thi-Qar, from 10/11/2021 to 3/2/2022, the period included field and laboratory stud. A total of 100, one day broilers Ross 308 were used. Laboratory analyzes were carried out in the laboratories of the Marshes Research Center, University of Thi-Qar and the laboratories of the College of Agriculture and Marshes, University of Thi-Qar. Three genotypes CC, CT and TT were identified whose frequency was 0.52, 0.34 and 0.14 respectively and the frequency of the C allele was 0.69 and the frequency of the T allele was 0.31. There were significant differences on Water Holding Capacity (WHC) among the genotypes, the CC genotype was superior to the CT and TT genotypes. As for the thawing and cooking loss, there were no significant differences between the genotypes. A significant differences on pH values among the genotypes, the CC genotype was superior to the CT and TT genotypes. Significant differences in flavor between genotypes, the CC genotype was superior to the CT genotype, and the CT genotype was superior to the TT genotype. Highly significant differences in tenderness, the CC genotype was superior to the TT genotype, while the CT genotype did not differ significantly from the CC and TT genotypes. Highly significant differences between the genotypes in juiciness, the CC genotype was superior to the CT and TT genotypes. No significant differences in color and general acceptable.
本研究于2021年10月11日至2022年3月2日在扎喀尔大学农业与沼泽学院动物生产系家禽场进行,期间包括田间和实验室种畜。试验选用1日龄罗斯308肉鸡100只。实验室分析在扎喀尔大学沼泽研究中心实验室和扎喀尔大学农业与沼泽学院实验室进行。鉴定出CC、CT和TT 3种基因型,频率分别为0.52、0.34和0.14,其中C等位基因频率为0.69,T等位基因频率为0.31。不同基因型间持水能力差异显著,CC基因型优于CT和TT基因型。在解冻和蒸煮损失方面,基因型间差异不显著。基因型间pH值差异显著,CC基因型优于CT和TT基因型。基因型间风味差异显著,CC基因型优于CT基因型,CT基因型优于TT基因型。在压痛程度上,CC基因型优于TT基因型,而CT基因型与CC和TT基因型差异不显著。多汁性基因型间差异极显著,CC基因型优于CT和TT基因型。颜色无明显差异,一般可接受。
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引用次数: 1
Study of the allelic frequency of HSPA6 gene and its genotypes and their effect on some characteristics of milk production in Holstein cows 荷斯坦奶牛HSPA6基因等位基因频率及其基因型的研究及其对奶牛产奶量的影响
Pub Date : 2022-11-03 DOI: 10.54174/utjagr.vo11.n2/13
Abdullah Hamid Salem, Ghadeer Razak Ahmed
The study was conducted at the Taj Al-Nahrain cattle station in Al-Diwaniyah Governorate, which is 200 km from the center of Dhi Qar Governorate. For the period from 10/15/2021 to 10/3/2022, 49 Holstein cows imported from Germany were used in this experiment, all in the third production cycle. And the laboratory part of the study was conducted in the laboratory of the Marsh Research Center Dhi Qar University for the purpose of separating the genetic material from cow blood samples and conducting genetic analysis on it and extracting the possible genotypes HSPA6 heat shock protein gene. The animals in the station were fed according to the season and the year in terms of the availability of fodder and the percentage of costs involved in raising the cows, as well as on the general state of the productivity of the cows, but in general they are provided with green fodder, which includes jet, barley, corn stalks and a percentage of each of the dry fodder (hay and hay). ) and concentrate (barley, bran and cake) according to what is available from them, as well as limestone and table salt. Data on milk production were collected from the station records, where the milk production was recorded weekly for the morning and evening fenugreek by the station, and the milk components were analyzed in the Abu Dairy Company Gharib / Research and Development Division by Milk Analyzer. 3 ml of blood was collected from the udder vein of each cow by 5 ml medical syringes, suitable for the size of the animal’s vein, and placed in collection tubes containing an anticoagulant substance (K2 EDTA) and transported in a cooler box for safekeeping Freezing at -18°C until extraction time. From the results obtained from this study, we can conclude that the mutation detected in the studied segment of the 6HSPA gene was a type of missense mutation and worked to change the code of the amino acid valine to the code of the amino acid leucine. Three genotypes were shown: GG, GT, and TT. It was found that there was a clear commonality of cows carrying the GG genotype with a rate of 88%, followed by the GT genotype with 8%, while the TT genotype got the lowest percentage and amounted to 4%, and with allelic recurrence Its value is 0.92 for the G allele and 0.08 for the T . allele Groups of cows carrying the genotypes (GG, GT and TT) did not differ significantly among themselves in the characteristics of milk production and its components, but an arithmetic superiority between the three genotypes was observed and referred to in all the studied traits.
这项研究是在Al-Diwaniyah省的Taj Al-Nahrain养牛场进行的,该养牛场距离迪卡尔省中心200公里。试验时间为2021年10月15日至2022年10月3日,选用德国进口荷斯坦奶牛49头,均处于第三生产周期。本研究的实验室部分在迪卡尔大学马什研究中心实验室进行,目的是从牛血液样本中分离遗传物质并对其进行遗传分析,提取可能的基因型HSPA6热休克蛋白基因。该站的动物是根据季节和年份,根据饲料的可得性和饲养奶牛的成本百分比,以及奶牛的一般生产力状况来喂养的,但一般来说,它们提供的是绿色饲料,其中包括喷气、大麦、玉米秸秆和每种干饲料的百分比(干草和干草)。并根据可获得的原料浓缩(大麦、麸皮和蛋糕),以及石灰石和食盐。从站点记录中收集产奶量数据,站点每周记录早晨和晚上葫芦巴的产奶量,并在Abu乳业公司Gharib /研发部门使用牛奶分析仪分析牛奶成分。用适合动物静脉大小的5 ml医用注射器从每头奶牛的乳房静脉中采集3 ml血液。放置在含有抗凝物质(K2 EDTA)的收集管中,并放入冷却盒中保存,在-18°C冷冻,直到提取时间。根据本研究的结果,我们可以得出结论,在6HSPA基因所研究的片段中检测到的突变是一种错义突变,其作用是将氨基酸缬氨酸的编码改变为氨基酸亮氨酸的编码。显示三种基因型:GG、GT和TT。结果发现,奶牛携带GG基因型的共性明显,为88%,其次是GT基因型,为8%,TT基因型的共性最低,为4%,且具有等位基因复发率,G等位基因复发率为0.92,T等位基因复发率为0.08。携带GG、GT和TT基因型奶牛的等位基因群之间在产奶量及其组成特征上差异不显著,但3个基因型之间在所有研究性状上均存在算术优势。
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引用次数: 0
Study of the growth of common carp Cyprinus carpio in muddy ponds using a local diet 用当地饲料饲养泥塘鲤鱼生长的研究
Pub Date : 2022-11-03 DOI: 10.54174/utjagr.vo11.n2/11
Zahraa Saeed Jabbar, Dr. Kamil Kadhim, Fahad
The current study examined the culture of the common carp Cyprinus carpio in three lakes supplied with water from Shatt Al-Badaa, a section of the Euphrates River, located in the Al-Rifai district, the village of Al-Nazim, between latitude 46.0703 in the south and a length of 31.5540N. 2021 and up to (23/2/2022) Three standardized treatments were used in the culture density of 1000 fish/m3 divided into three lakes equally and with an average weight of 20 ± g. A standardized local diet was used to feed the fish. The temperature during the experiment ranged between 14-28 (C) values. pH (7-8.5), dissolved oxygen concentration (6.7-9.0) mg/L. Salinity ranged between 0.2-0.3) g/liter. The results of the statistical analysis of the protein efficiency ratio outperformed the fish of the third treatment (62.210). The results of the statistical analysis indicated that the percentage of protein in the muscles of fish after the experiment was higher, reaching 5.76%, while the lowest percentage in the muscles of fish before the experiment was 3.61. The percentage of protein decreased at the beginning of the experiment. We notice an increase in the percentage of protein deposited in the fish body at the end of the experiment. The results of the experiment when analyzed on the basis of dry weight showed a difference in the percentage of fat in the chemical analysis of fish muscles before the experiment, 0.0048%, while the percentage of fat in the chemical analysis of fish muscles after the experiment was 39%. This may be due to the large size of the fish during the experiment period, in addition to its adoption in Feeding on the natural food found in the ponds. The current study showed an increase in the percentage of ash in fish muscles before the experiment on the basis of dry weight, which amounted to 5.49 %, and we note a decrease in the percentage of ash in fish muscles before the experiment, on the basis of dry weight, of 4.25%. The results of the study showed that carbohydrates increased at the end of the experiment (from 0.00069 to 0.485). The results of our current study showed that the survival rate of fish was not affected by the survival rate in the months of the beginning of the experiment, October and November, except in the last months, December, January and February
目前的研究在三个湖泊中考察了鲤鱼鲤鱼的养殖情况。这些湖泊的水源来自幼发拉底河的一段Shatt Al-Badaa,位于Al-Nazim村的Al-Rifai区,南纬46.0703,北纬31.5540。2021年至2022年(23/2/2022)养殖密度为1000尾/m3,平均体重为20±g,采用3种标准化处理,养殖密度为1000尾/m3,采用标准化当地饲料饲喂。实验期间的温度范围在14-28 (C)之间。pH(7-8.5),溶解氧浓度(6.7-9.0)mg/L。盐度在0.2-0.3 g/l之间。蛋白质效率比统计分析结果优于第三处理鱼(62.210)。统计分析结果表明,试验后鱼的肌肉中蛋白质含量较高,达到5.76%,而试验前鱼的肌肉中蛋白质含量最低,为3.61。蛋白质含量在实验开始时呈下降趋势。我们注意到在实验结束时,鱼体内沉积的蛋白质百分比有所增加。以干重为基础进行分析的实验结果显示,实验前鱼肌肉化学分析中的脂肪百分比相差0.0048%,而实验后鱼肌肉化学分析中的脂肪百分比相差39%。这可能是由于实验期间鱼的体型较大,以及它们以池塘中发现的天然食物为食。目前的研究表明,实验前鱼类肌肉中灰分的比例在干重基础上增加了5.49%,我们注意到实验前鱼类肌肉中灰分的比例在干重基础上减少了4.25%。研究结果表明,碳水化合物在实验结束时有所增加(从0.00069增加到0.485)。我们目前的研究结果表明,在实验开始的几个月,10月和11月,除了最后几个月,12月,1月和2月,鱼的存活率不受存活率的影响
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引用次数: 0
Studying the effect of the genotypes of the GH gene on blood parameters and some structural characteristics of Iraqi buffalo milk 研究生长激素基因型对伊拉克水牛奶血液参数和某些结构特征的影响
Pub Date : 2022-11-03 DOI: 10.54174/utjagr.vo11.n2/17
Mazen Majed Malbas Al-Hasnawi, Ali AL-Galiby
The samples of this study were collected from the private fields of buffalo breeders in the Al-Tar district of Dhi Qar Governorate / Karma Bani Said district, where 50 samples of milking female buffaloes were used in the experiment, with ages ranging from (2-3 years), this study was conducted in the laboratories of the College Agriculture and Marshes Department of Animal Production for the period from 15/11/2021 to 30/5/2022 with the aim of diagnosing the genetic morphology of the growth hormone gene in Iraqi buffaloes and its relationship to blood parameters and some characteristics of milk composition. Where the blood parameters measurements included the number of red blood cells, the number of white blood cells, the percentage of hemoglobin in the blood and the volume of the aggregated cells. As for the measurement of milk components, it included protein percentage, lactose sugar percentage, fat percentage, percentage of non-fat solids, milk density, freezing point, ash percentage and water percentage. Then DNA was extracted from animal blood samples in the Molecular Genetics Laboratory of For the Marshes Research Center / Dhi Qar University, the primer of the growth hormone gene was amplified and then the genotypes in the growth hormone gene were determined using (PCR) technique by electrophoresis with a relay device, and then the sequence of bases was analyzed using the sanger sequencing technique. And then study the relationship between the genetic structures and the traits mentioned above. The results of the polymerase chain reaction (PCR) analysis of the growth hormone gene showed the appearance of a bundle with a size of 1196 base pairs, and the appearance of two alleles, G and T, where their frequency was (0.64 and 0.36), respectively, and by three genotypes: GG, TT and GT, where the frequency of these structures reached genetic (0.60, 0.32 and 0.08), respectively. Whereas, the value of chi-square was 17.1, which indicates the mismatch of the observed and expected animals, which indicates the imbalance of the population, which was observed in the study from the genetic frequencies of the unbalanced population. A single silent mutation was also found at position 118 of the 1196-base-pair-long studied region in the non-coding region 2Intron and at site 611 of the complete gene. The results of the polymerase chain reaction (PCR) analysis of the growth hormone gene showed a bundle with a size of 1196 base pairs, and the appearance of two alleles, G and T, with three genotypes: GG, TT and GT. The appearance of a single silent mutation at position 118 of the 1196-base-pair-long studied region in the 2Intron noncoding region and at site 611 of the complete gene. The frequency of the G allele was 0.64, while the frequency of the T allele was 0.36, and the frequency of the genotypes GG, TT and GT was (0.60, 0.32 and 0.08), respectively. Whereas, the value of chi-square was 17.1, which indicates the mismatch of the observed and expected animal
本研究的样本是从迪加尔省Al-Tar区/ Karma Bani Said区水牛饲养者的私人田地中收集的,其中50只哺乳雌性水牛样本用于实验,年龄从(2-3岁);本研究于2021年11月15日至2022年5月30日在大学农业与沼泽动物生产系实验室进行,目的是诊断伊拉克水牛生长激素基因的遗传形态及其与血液参数和乳成分的一些特征的关系。其中血液参数测量包括红细胞的数量,白细胞的数量,血液中血红蛋白的百分比和聚集细胞的体积。牛奶成分的测定包括蛋白质百分比、乳糖百分比、脂肪百分比、非脂肪固形物百分比、牛奶密度、冰点、灰分百分比和水分百分比。然后在吉喀尔大学马什研究中心分子遗传学实验室从动物血液样本中提取DNA,扩增生长激素基因引物,用接力装置电泳(PCR)技术确定生长激素基因的基因型,再用sanger测序技术分析碱基序列。然后研究遗传结构与上述性状之间的关系。对生长激素基因进行聚合酶链反应(PCR)分析,结果显示出现了1196个碱基对大小的基因束,G和T两个等位基因出现频率分别为0.64和0.36,GG、TT和GT三个基因型出现频率分别为0.60、0.32和0.08。而卡方值为17.1,表明观察到的动物与期望的动物不匹配,表明种群的不平衡,这是本研究从不平衡种群的遗传频率观察到的。在非编码区2内含子1196个碱基对长的研究区域的118位和完整基因的611位也发现了一个沉默突变。该生长激素基因的聚合酶链反应(PCR)分析结果显示,该生长激素基因束大小为1196个碱基对,存在两个等位基因G和T,分别为GG、TT和GT三种基因型。在2内含子非编码区1196个碱基对的研究区118位和完整基因的611位出现了一个沉默突变。G等位基因频率为0.64,T等位基因频率为0.36,GG、TT和GT基因型频率分别为0.60、0.32和0.08。而卡方值为17.1,表明观察到的动物与期望的动物不匹配,表明种群的不平衡,这是本研究从不平衡种群的遗传频率观察到的。通过得到的结果发现,红细胞、血红蛋白百分比和压实细胞的体积不受基因型的影响,其中GT基因型记录的红细胞最高,为(7.11±0.69)* 106个细胞/ml。而GG基因型的血红蛋白和堆积血细胞体积分别为(13.022±2.076)g/dL和(40.07±6.23)%。白细胞受基因型影响显著(P < 0.05),其中GT基因型优于GG和TT两种基因型,其值为(9.225±0.317)* 103个细胞/ml。不同生长激素基因型对乳成分的影响较小,其中GT基因型的冷冻度和乳密度最高,分别为(0.430±0.025)m5和(27.400±2.540)g/cm3。GG基因型对乳糖、蛋白质和非脂肪固形物的贡献率最高,分别为5.180±1.302、3.398±0.141和18.138±2.322。脂肪、灰分和水分含量以TT基因型最高,分别为7.608±2.027、2.596±0.284和81.868±1.531 %。
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引用次数: 0
Some genetic and non-genetic factors affecting a number of carcass characteristics in sheep 一些影响绵羊胴体性状的遗传和非遗传因素
Pub Date : 2022-11-03 DOI: 10.54174/utjagr.vo11.n2/16
Abdullah Hameed Salim, Zaman Ali Munshid Al-Zayadi
This study was conducted in one of the animal islands stores (private sector) in Suq Al-Shuyoukh district, south of Dhi Qar Governorate, for the period from 5/11/2021 to 8/5/2022 on a sample of 49 local Iraqi sheep (Orabi). The experiment included Slaughtering sheep in the butcher’s shop and studying some characteristics of the carcass and taking a sample of meat for the purpose of calculating some of the chemical properties of the meat samples. It was found that the age of the animal had a significant and clear effect on most of the studied traits, while the animal’s sex had no significant effect in all the studied traits. A significant effect was observed in the color of the wool In each of the characteristics of the percentage of dressing, water carrying capacity and the index of fiber breakage in meat. The results showed the selection of red and black sheep for the characteristics of water carrying capacity and the evidence of fiber breakage, and the selection of sheep of black and white colors for the characteristic of the clearing ratio.
本研究于2021年11月5日至2022年8月5日期间在迪加尔省南部Suq Al-Shuyoukh区的一个动物岛商店(私营部门)进行,样本为49只伊拉克当地绵羊(Orabi)。该实验包括在肉店屠宰羊,研究胴体的一些特征,并取肉样本以计算肉样本的一些化学性质。实验发现,动物的年龄对研究的大部分性状都有显著而明确的影响,而动物的性别对研究的所有性状都没有显著影响。羊毛的颜色对肉质的整理率、携水能力和纤维断裂指数等指标均有显著影响。结果表明,以红、黑两色羊的携水能力和纤维断裂证据为特征,以黑、白两色羊的清除率为特征。
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引用次数: 0
Effect of glutathione and ascorbic acid on some physical characteristics of seedlings of grape plant Halawani cultivar Vitis vinifera L. 谷胱甘肽和抗坏血酸对葡萄幼苗某些物理特性的影响。
Pub Date : 2022-11-03 DOI: 10.54174/utjagr.vo11.n2/14
Mahmood S. Abdel-Wahed, Lina A. Hameed, Ahmed Salamjwar
This experiment was conducted in the saran-covered plastic house of the College of Agriculture and Marshes, Thi-Qar University, on 20.02.2022, to study the effect of spraying with glutathione and ascorbic acid on the physical and chemical properties of the grape plant Vitis vinifera L at one year age. The effect of glutathione compound at four concentrations (0, 75, 150, 225) mg.l-1 and ascorbic acid at four concentrations also (0, 50, 100, 150) mg.l-1, and the interaction between them was studied and implemented as a factorial experiment with two-factors (4×4×3) by following the randomized complete block design (RCBD) with three replications (48 seedlings per replicate). The averages were tested using the Revised least significant difference R.L.S.D at the probability level of 5%. The most important results obtained indicate that the effect of spraying with glutathione significantly on the studied vegetative characteristics, where the spraying treatment (G3) with a concentration of (225 mg l-1) was significantly superior to the control treatment (G0) in(Stem diameter, number of branches, dry weight, wet weight), which gave the highest averages, respectively ((24,901 mm, 7.942 branches. plant-1, 1.596 g, 0.403 g, 20.903), respectively. As for ascorbic acid, its effect was significant. In the studied vegetative traits, the spraying treatment (3C) with a concentration of (150 mg.L-1) was superior in (plant height (cm), number of leaves (leaf. plant-1), leaf area (cm2), which gave the highest averages, respectively ( 25.110 mm, 25.110 mm, 7.509 branches. plant-1,1.649 g, 0.392, 20.767).As for the two interactions (glutathione + ascorbic acid) at a concentration of (225 mg.l-1 + 150 mg.l-1), the treatments showed clear statistical differences, as the treatments excelled in the vegetative and chemical characteristics (stem diameter, number of branches, wet weight, weight). Dry, chlorophyll content of leaves) was a significant effect on the control treatment
本试验于2022年2月20日在吉喀尔大学农业与沼泽学院的塑料棚进行,研究喷施谷胱甘肽和抗坏血酸对一年生葡萄植株Vitis vinifera L理化特性的影响。谷胱甘肽化合物在4种浓度(0、75、150、225)mg下的作用。L-1和抗坏血酸四种浓度(0、50、100、150)mg。采用随机完全区组设计(RCBD), 3个重复(每个重复48株幼苗),采用双因素析因试验(4×4×3)对两者的相互作用进行研究。在5%的概率水平上,使用修正的最不显著差异R.L.S.D对平均值进行检验。结果表明,喷施谷胱甘肽对植株的营养特性有显著影响,喷施浓度为(225 mg l-1)的G3处理(茎粗、枝数、干重、湿重)均显著优于对照处理(G0),均数最高,分别为(24,901 mm, 7.942枝;Plant-1,分别为1.596 g、0.403 g、20.903)。对于抗坏血酸,其作用显著。在所研究的营养性状中,浓度为(150 mg.L-1)的喷施处理(3C)在株高(cm)、叶片数(叶数)、叶片数量(叶数)和叶片数量(叶数)方面均有显著优势。株1)、叶面积(cm2),平均枝数最高,分别为25.110 mm、25.110 mm、7.509枝。Plant-1,1.649 g, 0.392, 20.767)。至于两种相互作用(谷胱甘肽+抗坏血酸)浓度为(225mg。L-1 + 150 mg.l-1),各处理在茎粗、枝数、湿重、重等营养和化学性状上均表现出明显的统计学差异。叶片干、叶绿素含量对对照处理有显著影响
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引用次数: 1
期刊
University of Thi-Qar Journal of agricultural research
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