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Effect of feed dilution the with date kernels treated in different ways on some blood traits of broiler 不同处理方式下枣仁饲料稀释对肉鸡某些血液性状的影响
Pub Date : 2022-11-02 DOI: 10.54174/utjagr.vo11.n2/10
Muhammad Jodi Shahid, Zahra'a Razzaq Hussain
This experiment was conducted at the poultry field, Agricultural Research and Experiments Station, College of Agriculture and the Marshes, Thi Qar University, from 11/1/2021 to 12/12/2021, to determine the effect of diluting the feed with date kernels treated in different ways on some productive traits of broilers. A total of 270, one day, 40 gm chicks of Rose-308 were used. Chicks were randomly distributed to the five experimental treatments with three replicates (18 birds for each replicate), the diets were provided from the beginning of the second week until the end of the sixth week. The treatments were as follows: T1: Standard control diet (without dilution); T2: control diet diluted by 20% regular date seed pods for 7-42 days; T3: basal diet diluted with 20% date kernels, cooked for 7-42 days; T4: basal diet diluted with 20% vinegar-treated seed for 7-42 days; T5: basal diet diluted by 20% seed germinated for 7-42 days. The results indicated a significant increase on the cellular blood characteristics (Red Blood Cell Count, White Blood Cell Count, Hemoglobin concentration and the Packed Cell volume) in all dilution treatments with date kernel powder, whether treated or untreated. A significant increase on the concentration of albumin, globulin and total protein with a significant decrease in the concentration of glucose, cholesterol and triglycerides in the dilution treatments with date kernel powder compared to the control treatment.
本试验于2021年11月1日至2021年12月12日在Thi Qar大学农业与沼泽学院农业研究与实验站家禽场进行,研究不同处理方式的枣仁稀释饲料对肉鸡部分生产性状的影响。试验共使用玫瑰308鸡270只,每天40 gm。将雏鸡随机分为5个试验处理,每3个重复(每个重复18只),从第2周初至第6周末饲喂饲粮。处理方法如下:T1:标准对照饲粮(不稀释);T2:对照日粮,稀释20%普通枣子荚,7 ~ 42 d;T3:基础日粮中添加20%的枣仁,煮7-42天;T4:用20%醋处理过的种子稀释基础饲粮,7-42天;T5:基础饲粮稀释20%的种子发芽,7-42 d。结果表明,红枣仁粉在所有稀释处理中,无论是处理还是未处理,细胞血液特征(红细胞计数、白细胞计数、血红蛋白浓度和堆积细胞体积)均显著增加。与对照处理相比,枣仁粉稀释处理显著增加了白蛋白、球蛋白和总蛋白的浓度,显著降低了葡萄糖、胆固醇和甘油三酯的浓度。
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引用次数: 1
Study to detect cytotoxicity of The eggplant plant ( Solanum melongena L) 茄类植物细胞毒性检测的研究
Pub Date : 2022-11-02 DOI: 10.54174/utjagr.vo11.n2/04
Ahlam Husain Farhoud Khalawi
The current study included the cytotoxicity test of the active compounds isolated from the peels of the fruits of the eggplant plant, which is found in the local markets of Dhi Qar governorate and used for food and grown within the governorate and not imported during the period (July, August, September, October, November, December. January), the trend of human red blood cells for the purpose of determining the possibility of using them as drug alternatives in the future. The peels of the eggplant plant were extracted with hexane once, then the alkaloid extract was prepared from it again. The alkaloid compounds were detected using the Drankcroff reagent and Mayer's reagent. The positive result of the detection gave an indication of the presence of alkaloid substances that are antioxidants and have a high nutritional value. The concentrations of plant extracts represented by hexane eggplant extract and its alkaloid extract at concentrations (75,50,25,10,5,100) mg/ml were prepared using DMSO solution as a solvent, and a negative control factor containing physiological salt and blood, and a positive control factor (tap water) were prepared. ), and a third control factor consisted of blood solution with DMSO solvent, and the solution was monitored for (3-24) hours to observe the dissolution of blood cells. The cytotoxicity results of plant extracts of Solanum melongena L. showed that the concentrations (75,50,25,10,5,100) mg/ml did not cause any hemolytic and therefore do not cause any cytotoxicity towards red blood cells, and the bottom layer appeared in red color Which represents human blood, and the upper layer represents the extract as well as the physiological solution. If these compounds were toxic, the decomposition of red blood cells would result. Room temperature is 30°C and inside the incubator is 27°C. Incubator made by Dander, Germany and the concentration of the substance.
目前的研究包括从茄子植物果实的果皮中分离出的活性化合物的细胞毒性测试,这些植物在迪卡尔省的当地市场上发现,用于食品并在该省种植,在7月、8月、9月、10月、11月和12月期间没有进口。1月),研究人体红细胞的趋势,以确定将来将其用作药物替代品的可能性。用己烷对茄子皮进行一次提取,再对其进行生物碱提取。生物碱类化合物采用Drankcroff试剂和Mayer试剂进行检测。检测的阳性结果表明存在抗氧化剂和具有高营养价值的生物碱物质。以DMSO溶液为溶剂制备浓度为(75、50、25、10、5100)mg/ml的正己烷茄子提取物及其生物碱提取物为代表的植物提取物浓度,并制备含生理盐和血液的负控制因子和正控制因子(自来水)。第三个控制因素为含DMSO溶剂的血溶液,监测(3-24)小时,观察血细胞溶解情况。龙葵植物提取物的细胞毒性结果显示,浓度(75、50、25、10、5100)mg/ml均不产生溶血作用,因此对红细胞不产生细胞毒性,底部呈红色,代表人血,上层代表提取物和生理溶液。如果这些化合物有毒,就会导致红细胞分解。室温为30℃,培养箱内温度为27℃。培养箱由德国皮德尔公司制造,浓缩物质。
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引用次数: 0
Study of the Level of Gene Expression of the GLUT2 Glucose Vector in the Small Intestine of the Meat Broiler and its Relationship to Productive Traits 肉鸡小肠GLUT2葡萄糖载体基因表达水平及其与生产性状关系的研究
Pub Date : 2022-11-02 DOI: 10.54174/utjagr.vo11.n2/07
Anwar Hammood Obaid Al-Omar
The study was conducted in the field of animal production of the Agricultural Research Station / College of Agriculture and the Marshes / Dhi Qar University for the period from 10/11/2021 to 25/ 2/ 2022. Laboratory tests of the samples were carried out in the Molecular Genetics Laboratory of the College of Dentistry / Dhi Qar University, for the purpose of knowing the gene expression of the SLC2A2 gene encoding the glucose transporter GLUT2 and the abundance of its mRNA transcripts in different regions of the small intestine of broilers when fed at different levels of energy and to study the relationship of that abundance to some productive traits. In this study, 135 non-naturalized of meat broiler (breed 308 Ross) were used. The broiler were randomly distributed to three experimental treatments, with three replicates for each group (15 chicks/repeat), used the adlibitum free feeding system for the duration of the experiment, which lasted (35) days. The study included three levels of energy, where the first treatment was a control diet, which represented 3065 and 3133 kilocalories/kg for the primer and final diet, respectively, as for the second treatment, it contained a high level of energy 3200 kilocalories/kg and 3297 kilocalories/kg for the primer and final diets, respectively, the third treatment contained a low level of energy 2769 kilocalories/kg and 2802 kilocalories/kg for primer and growth diets, respectively. The results of the study showed the following:- The possibility of amplifying the studied gene and knowing its abundance and according to the primers used. The highest level of mRNA transcription of the GLUT2 gene in the small intestine of males was in the jejunum region (9.41) at a representative energy level of 3297 kilocalories/kg, as for the duodenum, the expression of GLUT2 gene was lower than in the jejunum (0.535), the GLUT2 gene expression in males was slightly more than in females in the ileum region (0.578) at a representative energy level of 3297 kcal/kg. Gene expression was positively and significantly associated with live weight, weight gain and feed consumption rates, but it was inversely related to the efficiency of food conversion in the jejunum and duodenal regions. The abundance of the glucose transporter GLUT2 gene increased with the increase in the energy level in the diet. Males gave a higher percentage of mRNA copies in all target organs compared to females. The activity of the gene responsible for the transfer of glucose can be modified through feeding and increasing energy levels and thus its reflection on the performance of birds. As for the productive traits, the differences were significant in the average live body weight and weight gain, as well as feed consumption compared with the control treatment, while the feed conversion efficiency did not show any significant differences.
该研究于2021年11月10日至2022年2月25日在迪尔卡尔大学农业研究站/农业学院和沼泽地区的动物生产领域进行。为了解不同能量水平饲粮条件下肉仔鸡小肠不同区域编码葡萄糖转运体GLUT2的SLC2A2基因的基因表达量及其mRNA转录本的丰度,并研究其丰度与某些生产性状的关系,在迪尔卡尔大学牙科学院分子遗传学实验室对样品进行了实验室检测。本试验选用非归化肉鸡(308 Ross) 135只。将肉鸡随机分为3个试验处理,每组3个重复(15只鸡/重复),试验期间采用加力脂免饲制,试验期为35 d。能源的研究包括三个层次,第一个治疗是控制饮食,代表3065和3133千卡/公斤底漆和最后的饮食,分别为第二次治疗,它含有高水平的能量3200千卡/公斤和3297千卡/公斤底漆和最终的饮食,分别第三治疗含有低水平的能量2769千卡/公斤和2802千卡/公斤底漆和增长饮食,分别。研究结果表明:-根据所使用的引物,有可能扩增所研究的基因并知道其丰度。雄性小肠GLUT2基因mRNA转录量最高的部位为空肠(9.41),代表能量水平为3297千卡/公斤;十二指肠GLUT2基因表达量低于空肠(0.535);雄性回肠GLUT2基因表达量略高于雌性(0.578),代表能量水平为3297千卡/公斤。基因表达与活重、增重和采食量呈显著正相关,与空肠和十二指肠的食物转化效率呈负相关。葡萄糖转运体GLUT2基因的丰度随着饮食中能量水平的增加而增加。与雌性相比,雄性在所有靶器官中提供了更高百分比的mRNA拷贝。负责葡萄糖转移的基因的活性可以通过喂养和增加能量水平来改变,从而反映鸟类的性能。在生产性状方面,与对照处理相比,平均活重、增重、耗料量差异显著,饲料转化率无显著差异。
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引用次数: 0
Response of broccoli, Brassica oleracea var. botrytis to the effect of hybrids and organic fertilizer K-FULV GROWER in some chemical an qualitative characteristics 花椰菜、甘蓝变种芽孢菌对杂交种和K-FULV有机肥影响的响应
Pub Date : 2022-11-02 DOI: 10.54174/utjagr.vo11.n2/05
Ihsan . J . Ethbeab, Aseel Muayyad Mahbas Al-Saeedi Ihsan Jali Azbib, Aseel . M. Al-Saeedi
The experiment was carried out in the greenhouses of the research journal of the College of Agriculture and the Marshes - Dhi Qar University  in the Mohiyah area in the city of Nasiriyah during the growth season 2022-2021. The experiment began on 1111 2021 and is dated 110 2 2022. Replicators and two years old. The first factor is the cauliflower cross here, CEZAR and ANAHAR, and the next factor is the Egyptian KFULV GROWT auger taco (11000 1500 1850) km. An experimental unit, and at the end of the experiment, the results of vegetative growth were recorded, and after analyzing the results statistically, the results showed the superiority of the hybrid CEZAR over the hybrid ANAHAR in the Egyptian clash, chlorophyll, phosphorous, potassium, magnesium, and boron. Vitamin C (the rates reached 79.17, 255% and 0.28 00 2.06 49000 001, 1663.79 and 16.74 share 100 upper Uran km from the opening difference concentration 150 are the Arab unit of organic position in the same studied traits, as the results of the statistical analysis showed the presence                                                                                                                                                                                                                     
该试验于2022-2021年生长季节在纳西里耶市Mohiyah地区的迪卡尔大学农业与沼泽学院研究期刊的温室中进行。实验开始于2021年11月11日,日期为2022年11月2日。复制者,两岁。第一个因素是这里的菜花交叉,CEZAR和ANAHAR,下一个因素是埃及KFULV grow螺旋钻塔克(11000 1500 1850)公里。一个实验单元,在实验结束时,记录营养生长的结果,并对结果进行统计分析,结果表明,杂交品种CEZAR在埃及冲突、叶绿素、磷、钾、镁、硼方面优于杂交品种ANAHAR。维生素C(占比分别为79.17、255%和0.28、2.06、49000、001、1663.79和16.74,距开度差浓度共100上Uran km)在同一研究性状中的有机位置为阿拉伯单位150,统计分析结果表明其存在
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引用次数: 1
Effect of omega-3 on some productive and eggs traits of Japanese quail omega-3对日本鹌鹑产蛋性状的影响
Pub Date : 2022-11-02 DOI: 10.54174/utjagr.vo11.n2/06
Muhammad Jodi Shahid, Anfal Saud Abdul-Razzaq Al-Aboudi
The study was conducted to determine the effect of adding omega-3 fatty acid to the quail's diet on some productive and egg quality traits. This experiment was conducted in the poultry field, Department of Animal Production, College of Agriculture and Marshes, University of Thi Qar, from 10/202021 to 02/14/2022. A total of 300 quail birds, 45 days age, were used, which was fed on three diet type, the first diet (control ration) without adding oil, the second ration was added to it with sunflower oil (0.01%), the third diet was added to omega-3 oil at a rate of (0.01%). The results showed that there were significant differences on the rate of egg production, egg weight and mass, feed conversion factor for egg production, egg shape index, yolk index, shell weight, yolk color and shell thickness for the treatment to which omega-3 oil was added in comparison with the rest of the treatments.
这项研究是为了确定在鹌鹑的饮食中添加omega-3脂肪酸对一些生产和蛋品质性状的影响。本试验于2021年10月至2022年2月14日在齐喀尔大学农业与沼泽学院动物生产系家禽场进行。试验选用45日龄鹌鹑300只,分为3种饲粮,第一种饲粮(对照日粮)不添加油,第二种日粮中添加0.01%的葵花籽油,第三种日粮中添加0.01%的omega-3油。结果表明:与其他处理相比,添加omega-3油处理的产蛋率、蛋重和蛋质量、产蛋饲料系数、蛋形指数、蛋黄指数、蛋壳重、蛋黄颜色和蛋壳厚度均有显著差异。
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引用次数: 0
The effect of adding different levels of local grass pea on some qualitative traits of Japanese quail eggs 添加不同水平地方豆荚对日本鹌鹑蛋某些品质性状的影响
Pub Date : 2022-11-02 DOI: 10.54174/utjagr.vo11.n2/08
M. J. Shahid, Doaa Talib, Munshid Kinanah, Iraq Qar
This experiment was conducted in the poultry field of the research station at the College of Agriculture and the Marshes of Thi-Qar University, from 1/11/2021 to 1/4/2022, to determine the effect of replacement different levels of grass pea seeds instead of soybean meal in the diet, and their effect on the growth parameters of Japanese quail (Coturnix coturnix). A total of 216 Japanese quail birds of 45 days old were used, distributed randomly to four experimental treatments and three replicates (18 birds/replicate). The treatments were as follows: T1: control transaction; T2: The birds were fed a diet containing 20% soybean meal with 10% local grass pea seeds; T3: The birds were fed a diet containing 10% soybean with 20% local grass pea seeds; T4: The birds were fed a diet containing 30% local grass pea seeds. The results of the study indicated that the addition of 20% grass pea seeds powder led to a significant improvement on the qualitative traits of Japanese quail eggs (yolk and albumin height, relative weight of yolk, albumin and shell, shell thickness, yolk and albumin index and Hough unit), compared to the level of addition of 30% of crushed safflower seeds. Keywords: local grass pea,  eggs qualitative, Japanese quail.
本试验于2021年11月1日至2022年4月1日在喀尔喀尔大学农业与沼泽学院研究站家禽场进行,研究饲粮中以不同水平的豆粕替代豆粕对日本鹌鹑(Coturnix Coturnix)生长参数的影响。选用45日龄日本鹌鹑216只,随机分为4个试验处理和3个重复(每个重复18只)。处理方式如下:T1:控制交易;T2:饲粮中添加20%豆粕和10%当地草豆种子;T3:饲粮中添加10%大豆和20%当地草豆种子;T4:饲粮中含有30%的当地草豆种子。结果表明,与添加30%的红花碎籽相比,添加20%的草豆籽粉显著改善了日本鹌鹑蛋的品质性状(蛋黄和白蛋白高度、蛋黄、白蛋白和壳的相对重量、壳厚度、蛋黄和白蛋白指数和霍夫单位)。关键词:地方豆荚,蛋质,鹌鹑。
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引用次数: 0
Study of the physical and chemical characteristics of the Euphrates River in the Al-Fadhliah district in Nasiriya governorate 在纳西里亚省Al-Fadhliah地区幼发拉底河的物理和化学特征的研究
Pub Date : 2022-11-02 DOI: 10.54174/utjagr.vo11.n2/09
Zaman M. Abdalwahad, Dr. Kamel K. Fahd
The current study dealt with the study of physical and chemical characteristics  to assess the environmental condition of the Euphrates River for the period from the 5th of September 2021 to the 5th of February 2022. The length of the Euphrates River is 2780 km from its branching point, the height of its source is 4500 m and the amount of water flowing in it is 818 square meters per second. Its source from (Turkey, Syria, Iraq),  Gazi(2004). It pours into the Arabian Gulf, passing through several districts and sub-districts belonging to the Dhi Qar Governorate, and the Euphrates River is surrounded by vast agricultural lands planted with field crops and palms. Three stations were selected for the study. The first station is located 167 km north of the Fadhliah district in the water supply site for the region, the second station is located eight kilometers from the first station after crossing the river to the Fadhliah district. The third station is located 13 kilometers from the second station. The abiotic environmental characteristics such as air and water temperature, light transmittance, salinity, dissolved oxygen, carbon dioxide, pH, alkalinity, total hardness, and biological oxygen requirement were measured regularly for a period of six months. The water temperature ranged (from 20-12.5) in the first and third stations, respectively. The light transmittance ranged between (23-32) cm. The lowest values of salinity (1.5 parts per thousand) were recorded during November in the first station. The highest was (5.72) parts per thousand in the third station in January. The values of dissolved oxygen showed a decrease in the hot months (September and October), and the lowest values were (7.4) mg/liter during November in the second station. The pH ranged between (7.9-8.5), and the highest concentrations of the hardness of water were recorded in January (450 mg/L).  
目前的研究涉及研究幼发拉底河的物理和化学特征,以评估2021年9月5日至2022年2月5日期间的环境状况。幼发拉底河从它的分支点开始的长度为2780公里,源头高度为4500米,其流量为每秒818平方米。其来源(土耳其,叙利亚,伊拉克),加齐(2004年)。它流入阿拉伯湾,经过几个属于迪卡尔省的区和分区,幼发拉底河被种植着大田作物和棕榈树的大片农田包围。选择了三个站点进行研究。第一个站位于Fadhliah区以北167公里处的供水站点,第二个站位于距第一个站8公里后过河至Fadhliah区。第三站距离第二站13公里。在六个月的时间里,定期测量空气和水的温度、透光率、盐度、溶解氧、二氧化碳、pH、碱度、总硬度和生物需氧量等非生物环境特征。第一站和第三站水温分别为20 ~ 12.5℃。透光率在(23-32)cm之间。第一个监测站在11月录得最低盐度(千分之1.5)。最高的是1月份的第三站(5.72)。溶解氧值在炎热月份(9月和10月)呈下降趋势,11月最低,为(7.4)mg/l。pH值在7.9 ~ 8.5之间,水体硬度最高值出现在1月份(450 mg/L)。
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引用次数: 1
LD50 and affective dose of Eruca sativa mill (gergeer) ethanolic extract 芥菜乙醇提取物的LD50及有效剂量
Pub Date : 2022-09-29 DOI: 10.54174/utjagr.vo11.n2/02
Rana Abdulla Salih, Zuhair Abdulkareem. Dawah
Cardiotoxicity is defined as drug-induced damage to the heart muscle, which can result in cardiac failure or cardiac arrest. Cardiotoxicity is a common side effect of traditional anticancer drugs such as doxorubicin, taxanes, 5-fluorouracil, and cyclophosphamide, as well as newer agents such as biological antibodies such as Nivo. Cardiotoxicity is also caused by the long-term use of neurologic/psychiatric medications. This study was designed to determine the effect of eruca sativa mill (Gergeer) leaves extract on cardiotoxicity induced by doxorubicin in rabbits. The fifty-four rabbits used in this study were obtained from a local rabbit breeder. Animals ranged between 1-1.3 years old, and their weight was approximately 1-2kg without specifying gender experiment Acute toxicity study, The pilot study to determine the LD50 dose of Eruca sativa mill (gergeer) by "up and down" method after acute exposure in rabbits, The effective dose of Eruca sativa mill (gergeer) was determined by using a simple line equation. The effective dose of Eruca sativa. troponin I determine the level of  proteins in the blood increase in heart attack,.The Troponin-I level of the control positive group revealed a significant increase as compared with G3, G4, G5, and the control negative group.The cTn-I level of the G3 significant increase is compared with treated G4,G5 group and control negative group with a significant decrease  is compared with G1, G2 group and control positive group ; while treated G4 ,G5 group significant decrease  is compared with treated G1,G2,G3 group and control positive, Log number dose-response curve and maximum effective dose the simple line equation revealed that the effective Eruca sativa dose of Troponin I level was (3.01 , 2.99 ,1.97 ,1.015, 1.030) (ng/ml) respectively, whereas the effective Eruca  sativa dose of Troponin I was 316 mg/kg. conclusion , The effective dose-response results asymptotic to 10 % of LD50, induced cardiac toxicity with DOX appear biochemically, cardiographycally and histopathologically, The Eruca sativa mill (gergeer) extract was effective protective for induced cardiac toxicity.
心脏毒性被定义为药物引起的心肌损伤,可导致心力衰竭或心脏骤停。心脏毒性是传统抗癌药物(如阿霉素、紫杉烷、5-氟尿嘧啶和环磷酰胺)以及较新的药物(如Nivo等生物抗体)的常见副作用。心脏毒性也可由长期使用神经/精神药物引起。本研究旨在探讨芥子叶提取物对阿霉素致兔心脏毒性的影响。本研究中使用的54只兔子是从当地的兔子饲养员那里获得的。实验动物年龄在1 ~ 1.3岁,体重约为1 ~ 2kg,性别不确定。实验急性毒性研究,采用“上下法”测定兔急性暴露后苦参的LD50剂量的初步研究,苦参的有效剂量采用简单直线方程确定。芥蓝的有效剂量。肌钙蛋白I决定了心脏病发作时血液中蛋白质的水平。对照阳性组与G3、G4、G5及对照阴性组相比,肌钙蛋白- 1水平明显升高。G3组ctn - 1水平较治疗后的G4、G5组和对照阴性组显著升高,较治疗后的G1、G2组和对照阳性组显著降低;而处理G4、G5组较处理G1、G2、G3组和对照阳性显著降低,对数剂量-反应曲线和最大有效剂量单线方程显示,苜蓿有效剂量肌钙蛋白I水平分别为(3.01、2.99、1.97、1.015、1.030)(ng/ml),而苜蓿有效剂量肌钙蛋白I为316 mg/kg。结论:有效剂量-反应结果渐近至LD50的10%,DOX诱导的心脏毒性表现为生化、心脏学和组织病理学,芥蓝提取物对诱导的心脏毒性具有有效的保护作用。
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引用次数: 1
Evaluation the Protective Effect of Capparis spinosa Fruits Hydroalcoholic Extract Against Nephrotoxicity Induced by Cisplatin in Rats 刺荆果水酒精提取物对顺铂所致大鼠肾毒性的保护作用评价
Pub Date : 2022-09-29 DOI: 10.54174/utjagr.vo11.n2/03
Ali H. Saliem, Sarmad Mohammed Hashim
The present study was carried out to evaluate the kidney abnormality changes, and nephrotoxicity  after using the cisplatine and investigate the protective effect of capparis spinosa fruit extract  against these effects and evaluate the kidney function test urea, cratenine, total protein tests and  histopathological changes of kidney . Twenty one (21) male rats were randomly divided into three groups (7 rats/ each). First group was received distilled water orally for 10 days and served as control group. Second group was received cisplatin only (10 mg/kg, i.p.) as a single dose at day 8 and served as a cisplatin treated group. Third group, was received capparis spinosa fruit extract (100 mg/kg B.W) for 10 days and 10 mg/kg cisplatin as a single dose at day 8 . The results showed that the animals that treated with cisplatine alone were severed from histopathological changes and significantly increasing in the level of (Urea, Cr and Total protein ). While the third group (that treated with hydroalcoholic extract of capparis spiosa fruit ethanol 70% ) showed decreasing in the histopathplogical changes in  kidney , also significantly decrease in the level of (Urea, Cr and Total protein ). In this study csplatine induced histopathplogical changes, alteration  and increasing in the level of (Urea, Cr and Total protein ),  while the ( hydroalcoholic extract of capparis spiosa fruit ethanol 70% ) was have ability to maintain  kidney function test .   
本研究旨在评价顺铂对大鼠肾脏异常变化及肾毒性的影响,探讨刺荆果提取物对这些影响的保护作用,并对肾脏功能、尿素、克拉拉汀、总蛋白及肾脏组织病理学变化进行评价。雄性大鼠21只,随机分为3组,每组7只。第一组患者口服蒸馏水10 d,作为对照组。第二组在第8天仅接受顺铂单剂量治疗(10mg /kg, i.p.),作为顺铂治疗组。第三组,连续10 d给予刺荆果提取物(100 mg/kg B.W),第8 d给予顺铂10 mg/kg单剂量。结果表明,单用顺铂处理的大鼠组织病理改变基本消失,尿素、Cr和总蛋白水平明显升高。而第三组(荆刺果乙醇70%水酒精提取物处理组)肾脏组织病理变化明显降低,尿素、铬和总蛋白水平也显著降低。在本研究中,csplatine诱导大鼠肾组织病理改变,尿素、Cr和总蛋白水平改变和升高,而荆芥果乙醇70%水酒精提取物具有维持肾功能的能力。
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引用次数: 0
Oxidative stress induced cardiomyocytes injury by Tamoxifen and abrogates by zinc supplement in adult female rabbits 他莫昔芬对氧化应激诱导的成年母兔心肌细胞损伤及补锌对氧化应激的消除作用
Pub Date : 2022-09-29 DOI: 10.54174/utjagr.vo11.n2/01
Mohammed Ali, S. M., Ahmed E. Abood
Tamoxifen (TAM) is used in breast cancer chemotherapy since its approval by the Food and  Drug Administration in 1977. However, TAM therapy is accompanied with cardiomyocytes apoptosis. Oxidative stress and inflammation are the major implicated mechanisms contributing to TAM apoptosis. In this study, we explored whether zinc (Zn) supplementation could prevent TAM-induced cardiomyocytes injury in adult female rabbits. Rabbits were received zinc orally (0.3 mg/ kg body weight / day for 42 days) in zinc group, Tamoxifen group were received orally (0.25 mg/ kg body weight for 42 days), and combination (Zn+Tam) group were received equal dose from zinc and tamoxifen. To study the effect of the tamoxifen (selective estrogen receptor modulator) on the cardiac muscle. Results showed of histopathological study of heart taken from zinc treated group not showed any important abnormalities in histological appearance, tamoxifen group in heart tissue showed a massive necrotic tissue  and a huge inflammatory cell infiltration to damaged area and combination group lesion showed active inflammatory process which included blood vessels congestion, edema formation,  inflammatory cell aggregation especially macrophage and Myocyte vacuolization. As a consequence, the finding of the research Tam induction over production of reactive oxygen species (ROS) within cardiac cell and the imbalance between the ROS generating system and the antioxidant system can lead to a potentially harmful to tissues and cells.
他莫昔芬(TAM)自1977年获得美国食品和药物管理局批准以来,一直用于乳腺癌化疗。然而,TAM治疗伴随着心肌细胞凋亡。氧化应激和炎症是TAM细胞凋亡的主要机制。在这项研究中,我们探讨了锌(Zn)的补充是否可以预防tam诱导的成年雌性兔心肌细胞损伤。锌组家兔口服锌(0.3 mg/ kg体重/天,连用42 d),他莫昔芬组家兔口服锌(0.25 mg/ kg体重,连用42 d),锌+他莫昔芬联合(Zn+Tam)组家兔口服锌和他莫昔芬等剂量。目的:研究选择性雌激素受体调节剂他莫昔芬对心肌的影响。结果锌处理组心脏组织病理检查未见明显组织学异常,他莫昔芬组心脏组织出现大量坏死组织,损伤部位有大量炎性细胞浸润,联合用药组病变部位炎症反应活跃,包括血管充血、水肿形成,炎性细胞聚集,尤其是巨噬细胞和肌细胞空泡化。因此,研究发现心脏细胞内活性氧(ROS)的过度产生以及ROS生成系统与抗氧化系统之间的不平衡可能导致对组织和细胞的潜在危害。
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University of Thi-Qar Journal of agricultural research
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