Single-cell sequencing technology has developed rapidly in recent years and has demonstrated its power in many fields, especially in the field of tumor-, immunity-, and stem cell-related researches, where it explains many biological laws that remained uncovered. In terms of cardiovascular disease, in spite of application in studies of atherosclerotic plaque and explaining the cellular heterogeneity in plaque formation, single-cell sequencing technology is relatively limited and is in its infancy. This article reviews the application of single-cell sequencing technology in cardiovascular field in recent years and makes a brief comparison with its application in other fields, hoping to point out the way for future research.
{"title":"Application of single-cell sequencing technology in the study of cardiovascular development and diseases","authors":"Chuxiang Lei, Siliang Chen, Yue-hong Zheng","doi":"10.4103/TS.TS_2_19","DOIUrl":"https://doi.org/10.4103/TS.TS_2_19","url":null,"abstract":"Single-cell sequencing technology has developed rapidly in recent years and has demonstrated its power in many fields, especially in the field of tumor-, immunity-, and stem cell-related researches, where it explains many biological laws that remained uncovered. In terms of cardiovascular disease, in spite of application in studies of atherosclerotic plaque and explaining the cellular heterogeneity in plaque formation, single-cell sequencing technology is relatively limited and is in its infancy. This article reviews the application of single-cell sequencing technology in cardiovascular field in recent years and makes a brief comparison with its application in other fields, hoping to point out the way for future research.","PeriodicalId":102077,"journal":{"name":"Translational Surgery","volume":"352 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131459936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: This study evaluated noninvasive methods for the diagnosis and classification of lower-extremity arterial occlusive disease (LEAOD), specifically, plasma homocysteine (pHcy), ankle–brachial index (ABI), and brachial-ankle pulse wave velocity (baPWV). Materials and Methods: The study involved 102 patients with intermittent claudication treated at Beijing Chao Yang Hospital from 2010 to 2011. The affected arteries were assessed by computed tomography angiography (CTA) and categorized depending on the degree of occlusion as normal, stenotic, or occluded. ABI, pHcy, and baPWV were measured and compared among the groups. Factors that can affect ABI readings were analyzed. Results: Compared with patients in the normal group, the stenotic and occluded patients had significantly higher pHcy and baPWV, and lower ABI levels. The pHcy levels of the stenotic and occluded groups were similar. While statistically significant different ABI and baPWV levels were shown between the stenotic and occluded groups. The t values were 9.43 and 3.76, and the P < 0.001 and 0.001, respectively. Hypertension, diabetes mellitus, and blood cholesterol, C-reaction protein, and pHcy levels can influence the ABI value. Conclusion: ABI, pHcy, and baPWV values correlated with the results of the CTA examination with regard to LEAOD classification. ABI and baPWV may be useful for the categorical diagnosis of the disease. These findings contribute to the early diagnosis of LEAOD using noninvasive methods.
{"title":"Ankle–Brachial Index, plasma homocysteine, and brachial-ankle pulse wave velocity are important indicators in the diagnosis of early stage lower-extremity arterial occlusive disease","authors":"Tan Li, Yang Zhang, Wang-de Zhang","doi":"10.4103/TS.TS_6_19","DOIUrl":"https://doi.org/10.4103/TS.TS_6_19","url":null,"abstract":"Background: This study evaluated noninvasive methods for the diagnosis and classification of lower-extremity arterial occlusive disease (LEAOD), specifically, plasma homocysteine (pHcy), ankle–brachial index (ABI), and brachial-ankle pulse wave velocity (baPWV). Materials and Methods: The study involved 102 patients with intermittent claudication treated at Beijing Chao Yang Hospital from 2010 to 2011. The affected arteries were assessed by computed tomography angiography (CTA) and categorized depending on the degree of occlusion as normal, stenotic, or occluded. ABI, pHcy, and baPWV were measured and compared among the groups. Factors that can affect ABI readings were analyzed. Results: Compared with patients in the normal group, the stenotic and occluded patients had significantly higher pHcy and baPWV, and lower ABI levels. The pHcy levels of the stenotic and occluded groups were similar. While statistically significant different ABI and baPWV levels were shown between the stenotic and occluded groups. The t values were 9.43 and 3.76, and the P < 0.001 and 0.001, respectively. Hypertension, diabetes mellitus, and blood cholesterol, C-reaction protein, and pHcy levels can influence the ABI value. Conclusion: ABI, pHcy, and baPWV values correlated with the results of the CTA examination with regard to LEAOD classification. ABI and baPWV may be useful for the categorical diagnosis of the disease. These findings contribute to the early diagnosis of LEAOD using noninvasive methods.","PeriodicalId":102077,"journal":{"name":"Translational Surgery","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122844144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D. Gan, Wei Jin, Ying Li, Chang-Peng Han, Zhen-yi Wang
Crohn's disease (CD) is an inflammatory disease that occurs in unexplained gastrointestinal mucosa. Anal fistula is a common complication of CD. Anal fistula accounts for 15% of patients with ileocolonic CD, and 92% of CD patients with colon and rectum involvement have anal fistula. Although perianal CD is an anorectal benign disease, it significantly affects the patients' quality of life. Medical and surgical management are two major treatment approaches for Crohn's anal fistula. Drug treatment includes antibiotics, immunological agents, and biological agents. Nearly 30% of patients with CD anal fistula can be cured by standard medical treatment, and 70% need surgical intervention. The purpose of the current surgical treatment is to alleviate local symptoms and protect the anal sphincter function. The surgical treatment of Crohn's anal fistula depends largely on the type of anal fistula, the relationship with the sphincter, and whether it is in the remission of CD.
{"title":"Surgical Treatment for perianal Crohn's disease","authors":"D. Gan, Wei Jin, Ying Li, Chang-Peng Han, Zhen-yi Wang","doi":"10.4103/TS.TS_19_18","DOIUrl":"https://doi.org/10.4103/TS.TS_19_18","url":null,"abstract":"Crohn's disease (CD) is an inflammatory disease that occurs in unexplained gastrointestinal mucosa. Anal fistula is a common complication of CD. Anal fistula accounts for 15% of patients with ileocolonic CD, and 92% of CD patients with colon and rectum involvement have anal fistula. Although perianal CD is an anorectal benign disease, it significantly affects the patients' quality of life. Medical and surgical management are two major treatment approaches for Crohn's anal fistula. Drug treatment includes antibiotics, immunological agents, and biological agents. Nearly 30% of patients with CD anal fistula can be cured by standard medical treatment, and 70% need surgical intervention. The purpose of the current surgical treatment is to alleviate local symptoms and protect the anal sphincter function. The surgical treatment of Crohn's anal fistula depends largely on the type of anal fistula, the relationship with the sphincter, and whether it is in the remission of CD.","PeriodicalId":102077,"journal":{"name":"Translational Surgery","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114226667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hongbo Zhang, M. Bai, Zhiqi Chen, Zhengliang Li, Shizhong Zhang, Wen Li
Moyamoya disease (MMD), also known as abnormal intracranial vascular network, is a group of chronic progressive stenosis or occlusion of bilateral main branches of Willis ring (siphon segment of internal carotid artery and anterior and middle cerebral arteries, sometimes including the origin of posterior cerebral artery), followed by abnormal collateral small vascular network. The disease is known as MMD because it presents many dense piles of small blood vessels that appear as “puff of smoke” on cerebral angiogram. At present, great progress has been made in the field of epidemiology, pathogenesis, clinical manifestations, diagnosis, and treatment of adult MMD. However, the disease characteristics considerably differ in children. Therefore, this article will review the diagnosis, treatment, and prognosis of MMD in children.
{"title":"Progress of diagnosis and surgical treatment in children with moyamoya disease","authors":"Hongbo Zhang, M. Bai, Zhiqi Chen, Zhengliang Li, Shizhong Zhang, Wen Li","doi":"10.4103/TS.TS_17_18","DOIUrl":"https://doi.org/10.4103/TS.TS_17_18","url":null,"abstract":"Moyamoya disease (MMD), also known as abnormal intracranial vascular network, is a group of chronic progressive stenosis or occlusion of bilateral main branches of Willis ring (siphon segment of internal carotid artery and anterior and middle cerebral arteries, sometimes including the origin of posterior cerebral artery), followed by abnormal collateral small vascular network. The disease is known as MMD because it presents many dense piles of small blood vessels that appear as “puff of smoke” on cerebral angiogram. At present, great progress has been made in the field of epidemiology, pathogenesis, clinical manifestations, diagnosis, and treatment of adult MMD. However, the disease characteristics considerably differ in children. Therefore, this article will review the diagnosis, treatment, and prognosis of MMD in children.","PeriodicalId":102077,"journal":{"name":"Translational Surgery","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125139388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xipeng Wang, Bing Yuan, Mitsuteru Yokoyama, Ping Liu
Aim: Hepcidin is a hepatocyte-derived peptide that is thought to be involved in the regulation of intestinal iron absorption. Ferric citrate (FC) is a phosphate binder with additional effects on iron absorption. Better understanding of iron absorption under various levels of hepcidin may improve FC supplementation in individuals with renal anemia. We provide a new method to predict the individual iron absorption ability of the duodenum. Methods: Rats on an ordinary diet were given 10 mg of FC, and the serum concentrations of hepcidin and iron were monitored for 24 h. Rats with hepcidin levels induced by using alternative methods such as bloodletting or intravenous iron loading were also given FC, and serum iron level was measured at 2 h after oral iron intake (2-h oral iron absorption test). Results: Serum iron level increased constantly within 2 h after oral iron intake, and serum hepcidin level peaked 4 h after the iron level peaked. In the oral iron absorption test, the hepcidin levels inversely correlated with increased serum iron levels and hepcidin expression levels of >80 ng/mL completely inhibited the increase in iron absorption. Conclusion: This study suggests that hepcidin expression may be a strong mediator to regulate iron absorption and that performing an oral iron absorption test with hepcidin may help improve oral iron dosing schedules in patients undergoing hemodialysis.
{"title":"Rapid increase in serum iron level after oral iron intake as an indicator of duodenal iron absorption and inverse regulation of iron absorption by hepcidin expression","authors":"Xipeng Wang, Bing Yuan, Mitsuteru Yokoyama, Ping Liu","doi":"10.4103/TS.TS_15_18","DOIUrl":"https://doi.org/10.4103/TS.TS_15_18","url":null,"abstract":"Aim: Hepcidin is a hepatocyte-derived peptide that is thought to be involved in the regulation of intestinal iron absorption. Ferric citrate (FC) is a phosphate binder with additional effects on iron absorption. Better understanding of iron absorption under various levels of hepcidin may improve FC supplementation in individuals with renal anemia. We provide a new method to predict the individual iron absorption ability of the duodenum. Methods: Rats on an ordinary diet were given 10 mg of FC, and the serum concentrations of hepcidin and iron were monitored for 24 h. Rats with hepcidin levels induced by using alternative methods such as bloodletting or intravenous iron loading were also given FC, and serum iron level was measured at 2 h after oral iron intake (2-h oral iron absorption test). Results: Serum iron level increased constantly within 2 h after oral iron intake, and serum hepcidin level peaked 4 h after the iron level peaked. In the oral iron absorption test, the hepcidin levels inversely correlated with increased serum iron levels and hepcidin expression levels of >80 ng/mL completely inhibited the increase in iron absorption. Conclusion: This study suggests that hepcidin expression may be a strong mediator to regulate iron absorption and that performing an oral iron absorption test with hepcidin may help improve oral iron dosing schedules in patients undergoing hemodialysis.","PeriodicalId":102077,"journal":{"name":"Translational Surgery","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132922127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aim: To investigate the effect of electroacupuncture and hyperbaric oxygen therapies on the awakening of coma patients with severe brain trauma. Methods: One hundred and twenty severe brain injury patients were enrolled and randomly divided into four groups: conventional treatment group, electroacupuncture treatment group, adjuvant hyperbaric oxygen treatment group, and adjuvant electroacupuncture plus hyperbaric oxygen treatment group, with 30 patients in each group. The changes in Glasgow Coma Scale (GCS) and Glasgow Outcome Scale were recorded posttreatment. Results: Posttreatment, the GCS of all the four groups was increased. The GCSs of electroacupuncture treatment group, hyperbaric oxygen treatment group, and electroacupuncture plus hyperbaric oxygen treatment group were significantly higher than control group (P < 0.05). There was no significant difference in GCSs between electroacupuncture group and hyperbaric oxygen group (P > 0.05). Further, combining electroacupuncture and hyperbaric oxygen treatment made no significant improvement in GCS over individual treatments (P > 0.05). Conclusion: In addition to conventional treatment, the use of electroacupuncture and hyperbaric oxygen therapies may have a beneficial effect on patients' nervous system and thus aid in the awakening of coma patients.
{"title":"Investigating the efficacy of electroacupuncture and hyperbaric oxygen therapies on the awakening of coma patients with severe brain trauma","authors":"Xuejian Wang, Yang Chen, Zhifeng Wang","doi":"10.4103/TS.TS_14_18","DOIUrl":"https://doi.org/10.4103/TS.TS_14_18","url":null,"abstract":"Aim: To investigate the effect of electroacupuncture and hyperbaric oxygen therapies on the awakening of coma patients with severe brain trauma. Methods: One hundred and twenty severe brain injury patients were enrolled and randomly divided into four groups: conventional treatment group, electroacupuncture treatment group, adjuvant hyperbaric oxygen treatment group, and adjuvant electroacupuncture plus hyperbaric oxygen treatment group, with 30 patients in each group. The changes in Glasgow Coma Scale (GCS) and Glasgow Outcome Scale were recorded posttreatment. Results: Posttreatment, the GCS of all the four groups was increased. The GCSs of electroacupuncture treatment group, hyperbaric oxygen treatment group, and electroacupuncture plus hyperbaric oxygen treatment group were significantly higher than control group (P < 0.05). There was no significant difference in GCSs between electroacupuncture group and hyperbaric oxygen group (P > 0.05). Further, combining electroacupuncture and hyperbaric oxygen treatment made no significant improvement in GCS over individual treatments (P > 0.05). Conclusion: In addition to conventional treatment, the use of electroacupuncture and hyperbaric oxygen therapies may have a beneficial effect on patients' nervous system and thus aid in the awakening of coma patients.","PeriodicalId":102077,"journal":{"name":"Translational Surgery","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114670715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aim: The study aims to investigate the efficacy of double-tube polycaprolactone (PCL)/β-tricalcium phosphate (β-TCP) bioceramic composites in the treatment of bone defects. Methods: Forty healthy female New Zealand white rabbits, aged 6–10 months and weighing about 3 kg, were randomly divided into observation group (n = 20) and control group (n = 20) according to the random number table. The animals in the observation group were implanted with double-tube PCL/β-TCP bioceramic composites, and the control animals were implanted with single-tube porous β-TCP bioceramic scaffolds. At 12 weeks' postoperatively, the osteogenic effects between two groups were compared by local X-ray film, local bone appearance, histology, osteogenic area per field, and compression strength measured after sacrifice. Results: At 12 weeks after surgery, X-ray, bone appearance, histological staining, and osteogenic area per field showed that the scores in the observation group were significantly better than the control group (P < 0.05). Furthermore, the compression strength of the regenerated bone in the observation group was significantly higher than that of the control group (P < 0.05). Conclusion: The double-tube PCL/β-TCP bioceramic composite has promising therapeutic effects, osteogenic effect and mechanical properties in the treatment of bone defects, and thus may be of clinical significance.
{"title":"The efficacy of double-tube polycaprolactone/β-tricalcium phosphate bioceramic composite in the treatment of bone defects","authors":"G. Guo, Gentao Fan, Xiao‐zhou Liu, Xin Shi, Guangxin Zhou, Xing Zhou","doi":"10.4103/TS.TS_16_18","DOIUrl":"https://doi.org/10.4103/TS.TS_16_18","url":null,"abstract":"Aim: The study aims to investigate the efficacy of double-tube polycaprolactone (PCL)/β-tricalcium phosphate (β-TCP) bioceramic composites in the treatment of bone defects. Methods: Forty healthy female New Zealand white rabbits, aged 6–10 months and weighing about 3 kg, were randomly divided into observation group (n = 20) and control group (n = 20) according to the random number table. The animals in the observation group were implanted with double-tube PCL/β-TCP bioceramic composites, and the control animals were implanted with single-tube porous β-TCP bioceramic scaffolds. At 12 weeks' postoperatively, the osteogenic effects between two groups were compared by local X-ray film, local bone appearance, histology, osteogenic area per field, and compression strength measured after sacrifice. Results: At 12 weeks after surgery, X-ray, bone appearance, histological staining, and osteogenic area per field showed that the scores in the observation group were significantly better than the control group (P < 0.05). Furthermore, the compression strength of the regenerated bone in the observation group was significantly higher than that of the control group (P < 0.05). Conclusion: The double-tube PCL/β-TCP bioceramic composite has promising therapeutic effects, osteogenic effect and mechanical properties in the treatment of bone defects, and thus may be of clinical significance.","PeriodicalId":102077,"journal":{"name":"Translational Surgery","volume":"33 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115141536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aim: The findings on ABO blood groups and risk of brain tumors are inconsistent and contradictory. The aim of this study was to investigate this association through a case–control study in the northeast of China. Methods: Between 2001 and 2016, a total of 2077 cases with glioma (n = 612), meningioma (n = 816), and pituitary adenoma (n = 649), and 2716 healthy controls were retrospectively included in this study. Data were statistically analyzed by comparing to the participants with blood type O; after adjusting for age, gender, smoking, and alcohol status. Results: A comparatively higher incidence of glioma was observed with blood type B and AB cases (adjusting odds ratio [OR] = 1.389 [1.087–1.777] and 95% confidence interval [CI] = 2.260 [1.572–3.250]). A comparatively lesser incidence of pituitary adenoma was observed with blood type B and AB cases (adjusting OR = 0.561 [0.445–0.706] and 95% CI = 0.358 [0.307–0.619]). Moreover, a comparatively lesser incidence of meningioma was observed with blood type B cases (adjusting OR and 95% CI of 0.577 [0.463–0.717]). Conclusion: Blood type B and AB could be associated with a higher incidence of glioma and lesser incidence of pituitary adenoma, with a decreased risk of meningioma in blood type B individuals. Future studies are needed to reconfirm our finding and explore the underlying mechanisms linking brain tumors to ABO blood types.
{"title":"ABO blood group system and risk of brain tumors: A retrospective case–control study in Northeast of China","authors":"Liang Chang, Shi-hong Zhao, Zhou Dan, Guofu Li, Dongzhi Zhang, Chunlei Tan, Chuanlu Jiang","doi":"10.4103/TS.TS_24_17","DOIUrl":"https://doi.org/10.4103/TS.TS_24_17","url":null,"abstract":"Aim: The findings on ABO blood groups and risk of brain tumors are inconsistent and contradictory. The aim of this study was to investigate this association through a case–control study in the northeast of China. Methods: Between 2001 and 2016, a total of 2077 cases with glioma (n = 612), meningioma (n = 816), and pituitary adenoma (n = 649), and 2716 healthy controls were retrospectively included in this study. Data were statistically analyzed by comparing to the participants with blood type O; after adjusting for age, gender, smoking, and alcohol status. Results: A comparatively higher incidence of glioma was observed with blood type B and AB cases (adjusting odds ratio [OR] = 1.389 [1.087–1.777] and 95% confidence interval [CI] = 2.260 [1.572–3.250]). A comparatively lesser incidence of pituitary adenoma was observed with blood type B and AB cases (adjusting OR = 0.561 [0.445–0.706] and 95% CI = 0.358 [0.307–0.619]). Moreover, a comparatively lesser incidence of meningioma was observed with blood type B cases (adjusting OR and 95% CI of 0.577 [0.463–0.717]). Conclusion: Blood type B and AB could be associated with a higher incidence of glioma and lesser incidence of pituitary adenoma, with a decreased risk of meningioma in blood type B individuals. Future studies are needed to reconfirm our finding and explore the underlying mechanisms linking brain tumors to ABO blood types.","PeriodicalId":102077,"journal":{"name":"Translational Surgery","volume":"55 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123103935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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